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- Guillan, A., et al. Show all 5 Authors
- Biotechnology letters 2000 v.22 no.15 pp. 1247-1250
- Saccharomyces cerevisiae; calcium alginate; enzyme stability; gels; genes; plasmids; protein synthesis; recombinant proteins
- ... Immobilisation of cells in a calcium alginate gel improved plasmid stability (up to 50%) and enzymatic expression (up to 57%) of a recombinant Saccharomyces cerevisiae over-expressing the homologous gene EXG1. The rate of segregational loss in the free cells was 14-fold higher than that of the immobilised cells. Recombinant protein synthesis requires reduced cofactors, which affect the redox balan ...