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- Author:
- Smith, H.; Xu, Y.; Quail, P.H.
- Source:
- Plant physiology 1997 v.114 no.2 pp. 637-641
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; seedlings; hypocotyls; phytochrome; dose response; red light; far-red light; genetic variation; genotype; etiolation
- Abstract:
- ... Using dichromatic radiation, we show that the actions of phytochromes A and B (phyA and phyB) in Arabidopsis thaliana are antagonistic in mediating red and far-red radiation effects on seedling de-etiolation and yet act in a complementary manner to regulate de-etiolation, irrespective of spectral composition. At low phytochrome photoequilibria inhibition of hypocotyl extension was strong, because ...
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- Author:
- Lilley, R.M.; Portis, A.R. Jr.
- Source:
- Plant physiology 1997 v.114 no.2 pp. 605-613
- ISSN:
- 0032-0889
- Subject:
- ribulose-bisphosphate carboxylase; dose response; polymerization; Spinacia oleracea; adenosine triphosphate; magnesium; biochemical pathways; anions; potassium; hydrolysis; actin; enzyme activity; adenosinetriphosphatase
- Abstract:
- ... The ATPase activity and fluorescence of ribulose-1,5-bisphosphate carboxylase oxygenase (Rubisco) activase were determined over a range of MgCl2, KCl, and activase concentrations. Both salts promoted ADP release from ATP and intrinsic fluorescence enhancement by adenosine 5'-[gamma-thio] triphosphate, but Mg2+ was about 10 times more effective than K+. ATPase and fluorescence enhancement both incr ...
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- Author:
- Gary, G.R.; Chauvin, L.P.; Sarhan, F.; Huner, N.P.A.
- Source:
- Plant physiology 1997 v.114 no.2 pp. 467-474
- ISSN:
- 0032-0889
- Subject:
- messenger RNA; dose response; redox reactions; net assimilation rate; light intensity; acclimation; Secale cereale; gene expression; transcription (genetics); frost resistance; photosynthesis; genes; temperature; plant morphology; chloroplasts
- Abstract:
- ... By comparing growth under five different temperature and irradiance regimes (20 degrees C and 800, 250, and 50 micromoles m-2 s-1 and 5 degrees C and 250 and 50 micromoles m-2 s-1), we have examined the effects of light, temperature, and the relative reduction state of photosystem II on plant morphology, freezing tolerance (lethal temperature at which freezing injury occurs [LT50]), transcript lev ...
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- Author:
- Bohland, C.; Balkenhohl, T.; Loers, G.; Feussner, I.; Grambow, H.J.
- Source:
- Plant physiology 1997 v.114 no.2 pp. 679-685
- ISSN:
- 0032-0889
- Subject:
- protein synthesis; dose response; chitosan; seedlings; jasmonic acid; linoleate 13S-lipoxygenase; Puccinia graminis; Triticum aestivum; leaves; plant pathogenic fungi; oligosaccharides; chitin; enzyme activity; isozymes; protein composition
- Abstract:
- ... A glycopeptide elicitor prepared from germ tubes of the rust fungus Puccinia graminis Pers. f. sp. tritici Erikss. & Henn (Pgt),as well as chitin oligosaccharides, chitosan, and methyl jasmonate (MJ) stimulated lipoxygenase (LOX) activity (E.C. 1.13.11.12) in wheat (Triticum aestivum) leaves. Immunoblot analysis using anti-LOX antibodies revealed the induction of 92- and 103-kD LOX species after P ...
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- Author:
- Trost, P.; Foscarini, S.; Preger, V.; Bonora, P.; Vitale, L.; Pupillo, P.
- Source:
- Plant physiology 1997 v.114 no.2 pp. 737-746
- ISSN:
- 0032-0889
- Subject:
- dose response; hypocotyls; NADP (coenzyme); oxidoreductases; Cucurbita pepo; enzyme inhibitors; binding sites; etiolation; plasma membrane; enzyme activity; protein composition
- Abstract:
- ... Quinone oxidoreductase activities dependent on pyridine nucleotides are associated with the plasma membrane (PM) in zucchini (Cucurbita pepo L.) hypocotyls. In the presence of NADPH, lipophilic ubiquinone homologs with up to three isoprenoid units were reduced by intact PM vesicles with a Km of 2 to 7 micromolars. Affinities for both NADPH and NADH were similar (Km of 62 and 51 micromolars, respec ...
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- Author:
- Holbrook, L.; Tung, P.; Ward, K.; Reid, D.M.; Abrams, S.; Lamb, N.; Quail, J.W.; Moloney, M.M.
- Source:
- Plant physiology 1997 v.114 no.2 pp. 419-428
- ISSN:
- 0032-0889
- Subject:
- Helianthus annuus; Brassica napus; measurement; chemical degradation; dose response; jasmonic acid; gene expression; Triticum aestivum; Nicotiana tabacum; esters; stereochemistry; enzyme activity; chemical structure; seed germination; transgenic plants; cotyledons; proteinase inhibitors; reporter genes; chemical constituents of plants; chlorophyll; beta-glucuronidase
- Abstract:
- ... The importance of the two chiral centers at C-3 and C-7 in the molecular structure of jasmonic acid in plant responses was investigated. We separated methyl jasmonate (MeJA) into (3R)- and (3S)-isomers with a fixed stereochemistry at C-3, but epimerization at C-7 is possible. The four isomers of the nonepimerizable analog 7-methyl MeJA were synthesized. These six esters and their corresponding aci ...
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- Author:
- Sivasankar, S.; Rothstein, S.; Oaks, A.
- Source:
- Plant physiology 1997 v.114 no.2 pp. 583-589
- ISSN:
- 0032-0889
- Subject:
- Zea mays; seedlings; roots; shoots; nitrate reduction; nitrogen; carbon; metabolites; asparagine; glutamine; sucrose; dose response; nutrient uptake; nitrate reductase; enzyme activity; nitrite reductase; aspartate-ammonia ligase; genes; gene expression; transcription (genetics)
- Abstract:
- ... The accumulation and reduction of nitrate in the presence of the nitrogen metabolites asparagine (Asn) and glutamine (Gln) and the carbon metabolite sucrose (Suc) were examined in maize (Zea mays L.) seedlings in an attempt to separate their effects on the nitrate uptake system and the nitrate reduction system. After 8 h of exposure to nitrate in the presence of 1 mM Asn, tissue nitrate accumulati ...
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- Author:
- Gorshkova, T.A.; Chemikosova, S.B.; Lozovaya, V.V.; Carpita, N.C.
- Source:
- Plant physiology 1997 v.114 no.2 pp. 723-729
- ISSN:
- 0032-0889
- Subject:
- plant development; galactans; biosynthesis; cell growth; radiolabeling; galactose; Linum usitatissimum; developmental stages; xylose; arabinose; dose response; phloem; cell wall components; cell differentiation; metabolites
- Abstract:
- ... We investigated the synthesis and turnover of cell wall polysaccharides of the flax (Linum usitatissimum L.) plant during development of the phloem fibers. One-month-old flax plants were exposed to a 40-min pulse with 14CO2 followedby 8-h, 24-h, and 1-month periods of chase with ambient CO2, and radioactivity in cell wall sugars was determined in various plant parts. The relative radioactivity of ...
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