Publication Year: 1997 / Source: 1997 v.94 no.12 / Subject: mutants

Author:: Gloaguen, I.; Costa, P.; Demartis, A.; Lazzaro, D.; Di Marco, A.; Graziani, R.; Paonessa, G.; Chen, F.; Rosenblum, C.I.; Ploeg, L.H.T. van der.
Source:: Proceedings of the National Academy of Sciences of the United States of America 1997 v.94 no.12 pp. 6456-6461
ISSN:: 0027-8424
Subject:: polypeptides; messenger RNA; hypothalamus; hormone receptors; blood glucose; mice; gene expression; body fat; overeating; obesity; mutants; insulin; diabetes; growth factors; hyperinsulinemia
Abstract:: ... Receptor subunits for the neurocytokine ciliary neurotrophic factor (CNTF) share sequence similarity with the receptor for leptin, an adipocyte-derived cytokine involved in body weight homeostasis. We report here that CNTF and leptin activate a similar pattern of STAT factors in neuronal cells, and that mRNAs for CNTF receptor subunits, similarly to the mRNA of leptin receptor, are localized in mo ...
DOI:: 10.1073/pnas.94.12.6456
PubMed:: 9177239
PubMed Central:: PMC21071
: http://dx.doi.org/10.1073/pnas.94.12.6456

Author:: Bonning, B.C.; Ward, V.K.; Van Meer, M.M.M.; Booth, T.F.; Hammock, B.D.
Source:: Proceedings of the National Academy of Sciences of the United States of America 1997 v.94 no.12 pp. 6007-6012
ISSN:: 0027-8424
Subject:: viral insecticides; Manduca sexta; insecticidal properties; Baculoviridae; lysosomes; mutagenesis; recombinant proteins; Lactuca sativa; carboxylic ester hydrolases; bioassays; mutants; cytochemistry; enzyme activity; infection
Abstract:: ... Juvenile hormone esterase (JHE; EC 3.1.1.1), which is intrinsically involved in regulation of development of some insect larvae, is rapidly removed from the hemolymph by the pericardial cells. Lys-29 and Lys-524, which are implicated in the degradation of JHE, were mutated to Arg. Neither the half-life of the modified JHE in the hemolymph nor the catalytic parameters were changed significantly, bu ...
DOI:: 10.1073/pnas.94.12.6007
PubMed:: 9177159
PubMed Central:: PMC20991
: http://dx.doi.org/10.1073/pnas.94.12.6007

Author:: Rogina, B.; Benzer, S.; Helfand, S.L.
Source:: Proceedings of the National Academy of Sciences of the United States of America 1997 v.94 no.12 pp. 6303-6306
ISSN:: 0027-8424
Subject:: temporal variation; recombinant DNA; longevity; mutation; beta-galactosidase; gene expression; histochemistry; mutants; reporter genes; Drosophila melanogaster; genetic markers
Abstract:: ... Mutations of the drop-dead gene in Drosophila melanogaster lead to striking early death of the adult animal. At different times, after emergence from the pupa, individual flies begin to stagger and, shortly thereafter, die. Anatomical examination reveals gross neuropathological lesions in the brain. The life span of flies mutant for the drop-dead gene is four to five times shorter than for normal ...
DOI:: 10.1073/pnas.94.12.6303
PubMed:: 9177212
PubMed Central:: PMC21044
: http://dx.doi.org/10.1073/pnas.94.12.6303

Author:: Giebel, B.; Campos-Ortega, J.A.
Source:: Proceedings of the National Academy of Sciences of the United States of America 1997 v.94 no.12 pp. 6250-6254
ISSN:: 0027-8424
Subject:: transcription factors; sense organs; phenotype; regulator genes; transgenic animals; setae (animal); mutants; DNA-binding proteins; embryogenesis; Drosophila melanogaster
Abstract:: ... The Enhancer of split [E(spl)] gene complex of Drosophila comprises seven related genes encoding a special type of basic helix-loop-helix proteins, the function of which is to suppress the neural developmental fate. One of these proteins is E(spl) itself. To gain insight into the structural requirements for E(spl) function, we have expressed a large number of deletion variants in transgenic flies. ...
DOI:: 10.1073/pnas.94.12.6250
PubMed:: 9177203
PubMed Central:: PMC21035
: http://dx.doi.org/10.1073/pnas.94.12.6250

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