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- Jacinto, T.; McGurl, B.; Franceschi, V.; Felano-Freier, J.; Ryan, C.A.
- Planta 1997 v.203 no.4 pp. 406-412
- Solanum lycopersicum var. lycopersicum; messenger RNA; promoter regions; stems; immunohistochemistry; transgenic plants; jasmonic acid; methyl jasmonate; gene expression; plant veins; mechanical damage; petioles; enzyme activity; beta-glucuronidase; plant proteins
- ... Tomato (Lycopersicon esculentum Mill. cv. Better Boy) plants were transformed with a fused gene containing a 2.2-kb promoter fragment of the tomato prosystemin gene and the coding region of the beta-glucuronidase (GUS) reporter gene. The transgenic plants exhibited a low constitutive level of prosystemin-beta-glucuronidase gene expression, assayed by histochemical staining and GUS enzyme activity, ...
- Blume, B.; Grierson, D.
- plant journal 1997 v.12 no.4 pp. 731-746
- ethylene; pericarp; seedlings; Nicotiana plumbaginifolia; promoter regions; Passalora fulva; Tobacco mosaic virus; jasmonic acid; gene expression; mechanical damage; petioles; multigene family; oxygenases; infection; messenger RNA; abscission; enzyme activity; histochemistry; Solanum lycopersicum var. lycopersicum; transgenic plants; Erysiphe; flowers; ripening; reporter genes; beta-glucuronidase
- ... The enzyme ACC oxidase, catalysing the last step in the biosynthesis of the plant hormone ethylene, is encoded by a small multigene family in tomato, comprising three members, LEACO1, LEACO2 and LEACO3. LEACO1 is the major gene expressed during ripening, leaf senescence, and wounding (Barry et al., 1996). To investigate the transcriptional regulation of ACC oxidase gene expression, chimeric fusion ...
- Holbrook, L.; Tung, P.; Ward, K.; Reid, D.M.; Abrams, S.; Lamb, N.; Quail, J.W.; Moloney, M.M.
- Plant physiology 1997 v.114 no.2 pp. 419-428
- Helianthus annuus; Brassica napus; measurement; chemical degradation; dose response; jasmonic acid; gene expression; Triticum aestivum; Nicotiana tabacum; esters; stereochemistry; enzyme activity; chemical structure; seed germination; transgenic plants; cotyledons; proteinase inhibitors; reporter genes; chemical constituents of plants; chlorophyll; beta-glucuronidase
- ... The importance of the two chiral centers at C-3 and C-7 in the molecular structure of jasmonic acid in plant responses was investigated. We separated methyl jasmonate (MeJA) into (3R)- and (3S)-isomers with a fixed stereochemistry at C-3, but epimerization at C-7 is possible. The four isomers of the nonepimerizable analog 7-methyl MeJA were synthesized. These six esters and their corresponding aci ...