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You searched for: Publication year rev 8003-1997 Remove constraint Publication year rev: 8003-1997 Subject beta-glucuronidase Remove constraint Subject: beta-glucuronidase
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1. A 146 bp fragment of the tobacco Lhcb1*2 promoter confers very-low-fluence, low-fluence and high-irradiance responses of phytochrome to a minimal CaMV 35S promoter

Author:
Pablo D. Cerdán; Roberto J. Staneloni; Jorge J. Casal; Rodolfo A. Sánchez
Source:
Plant molecular biology 1997 v.33 no.2 pp. 245-255
ISSN:
0167-4412
Subject:
Cauliflower mosaic virus; Nicotiana tabacum; beta-glucuronidase; far-red light; gene expression; genes; light intensity; phytochrome; promoter regions; red light; seedlings; tobacco; transgenic plants
Abstract:
... The occurrence of very-low-fluence responses (VLFR), low-fluence responses (LFR) and high-irradiance responses (HIR) of phytochrome was investigated for the expression of the gene of β-glucuronidase (gusA) under the control of the tobacco Lhcb1*2 promoter, in etiolated transgenic tobacco seedlings. The activity of β-glucuronidase (GUS) showed biphasic responses to the calculated proportion of Pf ...
DOI:
10.1023/A:1005784407282

2. Agrobacterium tumefaciens-mediated barley transformation

Author:
Tingay, S.; McElroy, D.; Kalla, R.; Fieg, S.; Wang, M.B.; Thornton, S.; Brettell, R.
Source:
plant journal 1997 v.11 no.6 pp. 1369-1376
ISSN:
0960-7412
Subject:
genetic transformation; phosphinothricin acetyltransferase; Hordeum vulgare; transgenic plants; embryo (plant); Agrobacterium radiobacter; plasmid vectors; explants; gene expression; glufosinate; progeny; reporter genes; herbicide resistance; enzyme activity; bilanafos; beta-glucuronidase
Abstract:
... Genetically transformed barley was produced by cocultivating immature embryo explants with Agrobacterium tumefaciens carrying a binary vector coding for chimaeric bacterial genes, bar and gus, and selecting for bialaphos-resistant cultures from which plants were regenerated. Integration of both genes was confirmed by gel blot hybridization analysis of DNA from the transformed plants and their prog ...
DOI:
10.1046/j.1365-313X.1997.11061369.x

3. Agrobacterium-mediated transformation of the terpenoid indole alkaloid-producing plant species Tabernaemontana pandacaqui

Author:
Lopes-Cardoso, M.I.; Meijer, A.H.; Hoge, J.H.C.
Source:
Plant cell reports 1997 v.17 no.2 pp. 150-154
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; terpenoids; Catharanthus roseus; biosynthesis; transgenic plants; Agrobacterium radiobacter; gene expression; indole alkaloids; reporter genes; Tabernaemontana pandacaqui; catalytic activity; beta-glucuronidase
Abstract:
... Plants of the Apocynaceae family produce a wide range of terpenoid indole alkaloids (TIAs) which have important pharmaceutical applications. Studies of the molecular mechanisms controlling TIA biosynthesis may eventually provide possibilities to improve product yield by genetic modification of plants or cell cultures. However, these studies suffer from the lack of transformation/regeneration proto ...
DOI:
10.1007/s002990050369

4. Analysis of SCARECROW expression using a rapid system for assessing transgene expression in Arabidopsis roots

Author:
Malamy, J.E.; Benfey, P.N.
Source:
plant journal 1997 v.12 no.4 pp. 957-963
ISSN:
0960-7412
Subject:
Arabidopsis thaliana; gene expression; roots; transgenic plants; callus; mutants; genetic transformation; reporter genes; beta-glucuronidase; promoter regions; histochemistry
Abstract:
... Although the introduction of foreign genes into Arabidopsis has become routine, the production of transgenic Arabidopsis plants still requires several months. A transgene expression system (TES) has been developed that allows characterization of gene expression patterns and the effects of foreign genes in the Arabidopsis root in 2-4 weeks. The method is based on regeneration of stably transformed ...
DOI:
10.1046/j.1365-313X.1997.12040957.x
PubMed:
9375406

5. Analysis of phosphoenolpyruvate carboxylase gene structure and expression in alfalfa nodules

Author:
Pathirana, M.S.; Samac, D.A.; Roeven, R.; Yoshioka, H.; Vance, C.P.; Gantt, J.S.
Source:
plant journal 1997 v.12 no.2 pp. 293-304
ISSN:
0960-7412
Subject:
plant vascular system; promoter regions; meristems; gene expression; petioles; introns; messenger RNA; complementary DNA; phosphoenolpyruvate carboxylase; root nodules; transcription (genetics); exons; histochemistry; transgenic plants; plasmid vectors; Medicago sativa; reporter genes; beta-glucuronidase
Abstract:
... Phosphoenolpyruvate carboxylase (PEPC) plays a crucial role in the assimilation of CO2 during symbiotic N2 fixation in legume root nodules. In this study, an alfalfa PEPC gene (PEPC-7), whose transcripts are found at elevated levels in nodules relative to either leaves or roots, has been isolated and characterized. The intron/exon structure of this gene is identical to that of most other plant PEP ...
DOI:
10.1046/j.1365-313X.1997.12020293.x

6. The Arabidopsis MALE STERILITY 2 protein shares similarity with reductases in elongation/condensation complexes

Author:
Aarts, M.G.M.; Hodge, R.; Kalantidis, K.; Florack, D.; Wilson, Z.A.; Mulligan, B.J.; Stiekema, W.J.; Scott, R.; Pereira, A.
Source:
plant journal 1997 v.12 no.3 pp. 615-623
ISSN:
0960-7412
Subject:
fatty acids; flowering; pollen; promoter regions; plant proteins; cell walls; gene expression; Nicotiana tabacum; cell wall components; Arabidopsis thaliana; fatty alcohols; spores; amino acid sequences; histochemistry; transgenic plants; oxidoreductases; reporter genes; beta-glucuronidase
Abstract:
... The Arabidopsis thaliana MALE STERILITY 2 (MS2) gene product is involved in male gametogenesis. The first abnormalities in pollen development of ms2 mutants are seen at the stage in microsporogenesis when microspores are released from tetrads. Expression of the MS2 gene is observed in tapetum of wild-type flowers at, and shortly after, the release of microspores from tetrads. The MS2 promoter cont ...
DOI:
10.1046/j.1365-313X.1997.00615.x
PubMed:
9351246

7. AtDMC1, the Arabidopsis homologue of the yeast DMC1 gene: characterization, transposon-induced allelic variation and meiosis-associated expression

Author:
Klimyuk, V.I.; Jones, J.D.G.
Source:
plant journal 1997 v.11 no.1 pp. 1-14
ISSN:
0960-7412
Subject:
alleles; pollen; promoter regions; transposons; gene expression; introns; Arabidopsis thaliana; ovules; messenger RNA; spores; complementary DNA; amino acid sequences; meiosis; exons; histochemistry; flowers; genetic variation; beta-glucuronidase
Abstract:
... Based on homologies between the yeast DMC1 and the lily LIM15 meiosis-specific genes, degenerate PCR primers were designed that amplified the Arabidopsis DMC1 gene (AtDMC1). AtDMC1 genomic DNA (8 kb) was sequenced, and the transcript was characterized by reverse transcriptase-polymerase chain reaction (RT-PCR) and by 5' and 3' RACE (rapid amplification of cDNA ends). The AtDMC1 gene contains 15 ex ...
DOI:
10.1046/j.1365-313X.1997.11010001.x
PubMed:
9025299

8. Biolistic transformation of haploid isolated microspores of barley (Hordeum vulgare L.)

Author:
Yao, Q.A.; Simion, E.; William, M.; Krochko, J.; Kasha, K.J.
Source:
Genome = 1997 v.40 no.4 pp. 570-581
ISSN:
0831-2796
Subject:
genetic transformation; recombinant DNA; germ cells; biolistics; Hordeum vulgare; phosphinothricin acetyltransferase; transgenic plants; reporter genes; herbicide resistance; inheritance (genetics); pollen; bilanafos; beta-glucuronidase
Abstract:
... Transgenic barley plants were produced by the direct delivery of plasmid DNA into isolated microspores of barley cv. Igri using high velocity microprojectiles. The plasmid pAHC25 contained the uidA and bar genes, each under the control of a maize Ubi1 promoter. Bombarded microspores were cultured and selected on solid medium containing varying concentrations (2-5 mg/L) of the Basta herbicide activ ...
DOI:
10.1139/g97-075
PubMed:
18464848

9. Cell-specific expression of the promoters of two nonlegume hemoglobin genes in a transgenic legume, Lotus corniculatus

Author:
Andersson, C.R.; Llewellyn, D.J.; Peacock, W.J.; Dennis, E.S.
Source:
Plant physiology 1997 v.113 no.1 pp. 45-57
ISSN:
0032-0889
Subject:
developmental stages; promoter regions; Parasponia; nitrogen fixation; gene expression; Casuarina glauca; Lotus corniculatus; leghemoglobin; root nodules; amino acid sequences; transcription (genetics); Rhizobium; enzyme activity; histochemistry; Trema; gene transfer; symbiosis; transgenic plants; hemoglobin; reporter genes; beta-glucuronidase
Abstract:
... The promoters of the hemoglobin genes from the nitrogen-fixing tree Parasponia andersonii and the related nonnitrogen-fixing Trema tomentosa both confer beta-glucuronidase reporter gene expression to the central zone of the nodules of a transgenic legume, Lotus corniculatus. beta-Glucuronidase expression was high in the uninfected interstitial cells and parenchyma of the surrounding boundary layer ...
PubMed:
9008386
PubMed Central:
PMC158114

10. Cell-to-cell trafficking of cucumber mosaic virus movement protein:green fluorescent protein fusion produced by biolistic gene bombardment in tobacco

Author:
Itaya, A.; Hickman, H.; Bao, Y.; Nelsen, R.; Ding, B.
Source:
plant journal 1997 v.12 no.5 pp. 1223-1230
ISSN:
0960-7412
Subject:
Nicotiana tabacum; Cucumber mosaic virus; viral proteins; biolistics; transgenic plants; leaves; reporter genes; plasmodesmata; beta-glucuronidase; animal proteins; recombinant proteins; Scyphozoa; translocation (plant physiology)
Abstract:
... Previous micro-injection studies showed that some recombinant viral movement proteins and plant proteins produced in and purified from Escherichia coli could traffic from cell to cell. However, the relevance of these findings obtained by micro-injecting proteins produced in E. coli to the real functions of these proteins when produced in planta has been questioned. In this study, specific gene con ...
DOI:
10.1046/j.1365-313X.1997.12051223.x

11. Changes in targeting efficiencies of proteins to plant microbodies caused by amino acid substitutions in the carboxy-terminal tripeptide

Author:
Hayashi, M.; Aoki, M.; Kondo, M.; Nishimura, M.
Source:
Plant & cell physiology 1997 v.38 no.6 pp. 759-768
ISSN:
0032-0781
Subject:
Arabidopsis thaliana; cotyledons; leaves; cytosol; peptides; protein transport; glyoxysomes; peroxisomes; beta-glucuronidase; transgenic plants; binding sites
Abstract:
... It has been demonstrated that the carboxyl terminus of microbody enzymes functions as a targeting signal to microbodies in higher plants. We have examined an ability of 24 carboxy-terminal amino acid sequences to facilitate the transport of a cytosolic passenger protein, beta-glucuronidase, into microbodies in green cotyledonary cells of transgenic Arabidopsis. Immunoelectron microscopic analysis ...
DOI:
10.1093/oxfordjournals.pcp.a029233
PubMed:
9249991

12. Characterization of proteins that interact with the GTP-bound form of the regulatory GTPase Ran in Arabidopsis

Author:
Haizel, T.; Merkle, T.; Pay, A.; Fejes, E.; Nagy, F.
Source:
plant journal 1997 v.11 no.1 pp. 93-103
ISSN:
0960-7412
Subject:
Arabidopsis thaliana; guanosinetriphosphatase; phenotype; complementary DNA; pyrophosphatases; transgenic plants; meristems; guanosine triphosphate; introns; binding proteins; gene expression; amino acid sequences; exons; molecular weight; cell cycle; beta-glucuronidase; plant proteins
Abstract:
... Ran, a small soluble GTP-binding protein, has been shown to be essential for the nuclear translocation of proteins and it is also thought to be involved in regulating cell cycle progression in mammalian and yeast cells. Genes encoding Ran-like proteins have been isolated from different higher plant species. Overexpression of plant Ran cDNAs, similarly to their mammalian/yeast homologues, suppresse ...
DOI:
10.1046/j.1365-313X.1997.11010093.x
PubMed:
9025305

13. Characterization of the targeted nuclear accumulation of GFP within the cells of transgenic plants

Author:
Grebenok, R.J.; Lambert, G.M.; Galbraith, D.W.
Source:
plant journal 1997 v.12 no.3 pp. 685-696
ISSN:
0960-7412
Subject:
Nicotiana tabacum; transgenic plants; cytoplasm; active transport; molecular weight; amino acid sequences; plant proteins; cytochemistry; spatial distribution; leaves; beta-glucuronidase; enzyme activity; animal proteins; Scyphozoa; green fluorescent protein; Aequorea victoria
Abstract:
... The soluble proteins of the nucleoplasm are synthesized on cytoplasmic ribosomes. Proteins larger than about 40 kDa are post-translationally targeted to the nucleus via energy-dependent processes, passing through the nuclear pore complex into the nucleoplasm. Targeting involves nuclear localization signals (NLSs) found within the primary sequences of the imported proteins. In higher plants, inform ...
DOI:
10.1046/j.1365-313X.1997.00685.x

14. Cinnamyl alcohol dehydrogenase: identification of new sites of promoter activity in transgenic poplar

Author:
Hawkins, S.; Samaj, J.; Lauvergeat, V.; Boudet, A.; Grima-Pettenati, J.
Source:
Plant physiology 1997 v.113 no.2 pp. 321-325
ISSN:
0032-0889
Subject:
alcohol dehydrogenase; gene transfer; promoter regions; hybrids; stems; Populus alba; transgenic plants; Eucalyptus gunnii; cinnamic acid; gene expression; genes; Populus tremula; enzyme activity; histochemistry; beta-glucuronidase
Abstract:
... Stem sections from poplar that were stably transformed with a eucalypt cinnamyl alcohol dehydrogenase promoter-beta-glucuronidase construct were prepared by using either a technique routinely used in herbaceous species or a technique designed to take into account the particular anatomy of woody plants. Although both preparation techniques confirmed the pattern of expression previously observed (C. ...

15. Cloning of the pumpkin ascorbate oxidase gene and analysis of a cis-acting region involved in induction by auxin

Author:
Kisu, Y.; Harada, Y.; Goto, M.; Esaka, M.
Source:
Plant & cell physiology 1997 v.38 no.5 pp. 631-637
ISSN:
0032-0781
Subject:
genetic code; beta-glucuronidase; ascorbate oxidase; DNA; Cucurbita; amino acid sequences; gene expression; reporter genes; enzyme activity; exons; promoter regions; introns
Abstract:
... A genomic clone encoding ascorbate oxidase was isolated from pumpkin (Cucurbita sp.). This gene is consisted of four exons and three introns. Analyses of the promoter fusion to beta-glucuronidase reporter gene by transient expression assay in pumpkin fruit tissues suggested the existence of a cis-acting region responsible for auxin regulation. ...
DOI:
10.1093/oxfordjournals.pcp.a029214
PubMed:
9210335

16. Colonization of tomato root by a non‐pathogenic strain of Fusarium oxysporum

Author:
CHANTAL OLIVAIN; CLAUDE ALABOUVETTE
Source:
new phytologist 1997 v.137 no.3 pp. 481-494
ISSN:
0028-646X
Subject:
cortex; cell walls; Fusarium oxysporum; defense mechanisms; hyphae; pathogenicity; reporter genes; roots; beta-glucuronidase; infection; Solanum lycopersicum var. lycopersicum
Abstract:
... A strain of non‐pathogenic Fusarium oxysporum Schlecht. emend, Snyd. & Hans. has been selected for its capacity to reduce the incidence of Fusarium wilt of tomato. Among the possible modes of action of this strain, competition with the pathogen for the colonization of the root surface and tissues has been proposed. In order to study the pattern of root colonization, young Lycopersicon etculentum M ...
DOI:
10.1046/j.1469-8137.1997.00855.x

17. Compromising early salicylic acid accumulation delays the hypersensitive response and increases viral dispersal during lesion establishment in TMV-infected tobacco

Author:
Mur, L.A.J.; Bi, Y.M.; Darby, R.M.; Firek, S.; Draper, J.
Source:
plant journal 1997 v.12 no.5 pp. 1113-1126
ISSN:
0960-7412
Subject:
Nicotiana tabacum; Tobacco mosaic virus; salicylic acid; defense mechanisms; gene expression; oxygenases; transgenic plants; necrosis; virulence; messenger RNA; beta-glucuronidase; reporter genes; pathogenesis; translocation (plant physiology); promoter regions
Abstract:
... To investigate the role of salicylic acid (SA) in the hypersensitive response (HR) its accumulation was compromised during different phases of lesion development by differential expression of a salicylate hydroxylase gene (SH-L). Constitutive suppression of SA accumulation was achieved by expression of a gene fusion between the CaMV35S promoter (35S) and SH-L. Using the H2O2-responsive AoPR1 promo ...
DOI:
10.1046/j.1365-313X.1997.12051113.x
PubMed:
9418052

18. Cooperative of two distinct cis-acting elements is necessary for the S phase-specific activation of the wheat histone H3 promoter

Author:
Ohtsubo, N.; Nakayama, T.; Kaya, H.; Terada, R.; Shimamoto, K.; Meshi, T.; Iwabuchi, M.
Source:
plant journal 1997 v.11 no.6 pp. 1219-1225
ISSN:
0960-7412
Subject:
promoter regions; messenger RNA; histones; Oryza sativa; transgenic plants; plasmid vectors; mutation; gene expression; Triticum aestivum; interphase; beta-glucuronidase
Abstract:
... We have previously shown that the proximal promoter region (-185 to +57) of the wheat histone H3 gene (TH012) is sufficient for regulating S phase-expression of a reporter GUS gene. To define the cis-acting element(s) responsible for S phase-specific expression, GUS fusion genes under the control of wild-type or variously mutated H3 promoters were stably introduced into cultured rice Oc cells and ...
DOI:
10.1046/j.1365-313X.1997.11061219.x

19. Cutinase A of Botrytis cinerea is expressed, but not essential, during penetration of gerbera and tomato

Author:
Kan, J.A.L. van; Klooster, J.W. van't.; Wagemakers, C.A.M.; Dees, D.C.T.; Vlugt-Bergmans, C.J.B. van der.
Source:
Molecular plant-microbe interactions 1997 v.10 no.1 pp. 30-38
ISSN:
0894-0282
Subject:
genetic transformation; promoter regions; recombinant DNA; phenotype; tubulin; Botrytis cinerea; Gerbera; fungal diseases of plants; DNA probes; flowers; gene expression; pathogenicity; histochemistry; mutants; reporter genes; esterases; beta-glucuronidase; infection
Abstract:
... The plant pathogen Botrytis cinerea can infect undamaged plant tissue directly by penetration of the cuticle. This penetration has been suggested to be enzyme-mediated, and an important role for cutinase in the infection process has been proposed. In this study the expression of the cutinase encoding gene cutA of B. cinerea was analyzed using a cutA promoter-GUS reporter gene fusion. Transformants ...
DOI:
10.1094/MPMI.1997.10.1.30
PubMed:
9002270

20. Detectable beta-glucuronidase activity in transgenic cranberry is affected by endogenous inhibitors and plant development

Author:
Serres, R.; McCown, B.; Zeldin, E.
Source:
Plant cell reports 1997 v.16 no.9 pp. 641-646
ISSN:
0721-7714
Subject:
Vaccinium macrocarpon; Nicotiana tabacum; leaves; genetic transformation; transgenic plants; gene transfer; beta-glucuronidase; enzyme activity; enzyme inhibitors; polyvidone; flavonols; anthocyanidins; plant extracts; histochemistry; reporter genes; gene expression; proanthocyanidins
Abstract:
... Extracts of cranberry, rich in flavonols and proanthocyanins, inactivated beta-glucuronidase (GUS) in assays using either purified bacterial GUS or preparations of transgenic tobacco (Nicotiana tabacum L.) or transgenic cranberry (Vaccinium macrocarpon Ait.) expressing the gusA gene. Histochemical GUS assays produced random and generally unpredictable staining. The addition of polyvinylpolypyrroli ...
DOI:
10.1007/BF01275507