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... The intercellular washing fluid (IWF) from leaves of sugar beet (Beta vulgaris L.) contains a number of proteins exhibiting in vitro antifungal activity against the devastating leaf pathogen Cercospora beticola (Sacc.). Among these, a potent antifungal peptide, designated IWF4, was identified. The 30-amino-acid residue sequence of IWF4 is rich in cysteines (6) and glycines (7) and has a highly bas ...
... Rapid reactions comprising efflux of K+ and Cl-, phosphorylation of a 63-kDa protein (pp63), extracellular alkalinization and synthesis of H2O2 are equally induced in cells of Picea abies (L.) Karst. by chitotetraose, colloidal chitin and cell wall elicitors from the ectomycorrhizal fungus Hebeloma crustuliniforme (Bull. ex Fries.) Quel. an ectomycorrhizal partner of spruce. Cleavage of fungal cel ...
... A well-defined X-ray fiber pattern of chitosan acetate was obtained by immersing a tendon chitosan, prepared from a crab tendon chitin by a solid-state N-deacetylation, in an aqueous acetic acid-isopropanol solution at 110°C. This pattern was very similar to that of chitosan salts with some inorganic acids, such as HF, HCl, and H₂SO₄, in which chitosan chains form an 8/5 helix, indicating that chi ...
... Six common edible fungi from four orders of Basidiomycetes, namely Agaricus bisporus (Agaricales), Auricularia auricula and Auricularia polytricha (Auriculariales), Tremella fuciformis (Tremellates), Ganoderma lucidum and Poria cocos (Aphyllophorales), were analysed for their total dietary fibre (TDF) content using the Association of Official Analytical Chemists (AOAC) method and for dietary fibre ...
... A glycopeptide elicitor prepared from germ tubes of the rust fungus Puccinia graminis Pers. f. sp. tritici Erikss. & Henn (Pgt),as well as chitin oligosaccharides, chitosan, and methyl jasmonate (MJ) stimulated lipoxygenase (LOX) activity (E.C. 1.13.11.12) in wheat (Triticum aestivum) leaves. Immunoblot analysis using anti-LOX antibodies revealed the induction of 92- and 103-kD LOX species after P ...
... The induction of plant defense-related responses by chitin oligomers and the Rhizobium meliloti lipo-chito-oligosaccharide nodulation signals (Nod factors) in Medicago cell cultures and roots was investigated by following the expression of genes encoding enzymes of the isoflavonoid biosynthetic pathway, such as chalcone synthase, chalcone reductase, isoflavone reductase, as well as genes encoding ...
... The effect of hexaflumuron, a chitin synthesis inhibitor, on the mating success of three species of vector mosquitoes, namely Culex quinquefasciatus, Aedes aegypti and Anopheles stephensi, was investigated. The three species were exposed to concentrations giving 5% and 50% inhibition of emergence (EI₅ and EI₅₀, respectively) at the fourth instar and to 0.005 and 0.05 mg /1 hexaflumuron at the pupa ...
... When a series of chitin oligosaccharides was added into a rice suspension culture, N-acetylchito- hexaose, N-acetylchitopentaose, and N-acetylchitotetraose caused an increase in extracellular chitinase activity, mainly due to induction of a class III chitinase. In the case of N-acetylchitohexaose, a substantial increase in the chitinase activity was observed at a concentration higher than 0.01 μg/ ...
... N-acetylchitooligosaccharides (fragments of chitin) elicit defense responses, including phytoalexin production, in suspension-cultured rice cells. They induced rapid and transient membrane depolarization accompanied by a transient increase in net Cl- -efflux. The membrane depolarization was not affected by anaerobiosis or azide, suggesting that the major part of the depolarization was mediated by ...
... The ¹⁵N natural abundance and N concentrations of fruit bodies from 70 species (23 genera) of ectomycorrhizal fungi found in boreal forests are presented. Large intraspecific and intrageneric differences were found, e.g. 8.3‰¹⁵N in the species Dermocybe crocea and 12.6‰ in the genus Cortinarius. In addition, significant differences in both δ¹⁵N and %N were found between different parts of fruit bo ...
shrimp; postharvest treatment; fish processing; waste utilization; chitin; fish products; protein hydrolysates; food quality; microbial contamination; shelf life; Indonesia
Enterobacter; Salvia hispanica; ammonium sulfate; biotechnology; calcium; carboxymethylcellulose; chitin; chitinase; chitosan; culture filtrates; fractionation; gel chromatography; isoelectric point; molecular weight; pH; polyacrylamide gel electrophoresis; temperature
Abstract:
... Entevobacter sp. G-1 which produces chitinolytic and chitosanolytic enzymes, was previously isolated in our laboratory. One major chitinase, designated ChiA, was purified 42.9-fold from a culture filtrate of Entevobacter sp. G-L To purify the chitinase, ammonium sulfate fractionation, DEAE-Sephadex A-50 column chromatography, and gel filtration on Sephadex G-100 column chromatography were used. Th ...
Arabidopsis thaliana; Vigna unguiculata; rhizosphere; chemical constituents of plants; calcium; Ensifer meliloti; root hairs; Rhizobium; nodulation; oligosaccharides; chitin; inorganic ions
Abstract:
... Rhizobia excrete variously substituted lipo-oligosaccharide Nod factors into the legume rhizosphere. Homologous legumes respond to these signals through deformation of the root hairs and the development of nodulation foci in the root cortex. Cellular events in root hairs from the susceptible zone of nearly mature root hairs were studied in root segments loaded with the calcium indicators Fura-2 or ...
proximate composition; broiler chickens; duodenum; raw foods; cecum; liveweight gain; amino acids; jejunum; length; digestible protein; ileum; feed conversion; corn; soybean meal; fish meal; autoclaving; chitin; diet; dosage; protein intake; protein content
Abstract:
... 1. The nutritive value of squilla meal, which contained 339 g crude protein, 18.6 g ether extract, 72.8 g calcium, 17.2 g phosphorus and 7.13 MJ of apparent metabolisable energy/kg, was examined in 5 experiments with broilers. 2. Squilla meal contained less lysine (3.45 vs 6.74 g/16g nitrogen) and methionine (1.02 vs 1.86/16 g nitrogen) than fish meal, had a lower protein digestibility coefficient ...
... Measurements of the chitin content of the rooting horizons of a typical mor‐humus heathland soil, indicate that chitin can contain in excess of 20% of the total nitrogen in the litter (L) horizon and 30% in the fermentation (F) horizon. Much of this chitin‐nitrogen is thought to be contained in the mycelial walls of soil fungi. Experiments were therefore designed to test the hypothesis that such s ...