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... 1,8-cineole is a volatile growth inhibitor produced bySalvia species. We examined the effect of this allelopathic compound on the growth of other plants usingBrassica campestris as the test plant. Cineole inhibited germination and growth ofB. campestris in a dosedependent manner. WhenB. campestris was grown for 5 days with various concentrations of cineole, the length of the roots was found to be ...
... The occurrence of very-low-fluence responses (VLFR), low-fluence responses (LFR) and high-irradiance responses (HIR) of phytochrome was investigated for the expression of the gene of β-glucuronidase (gusA) under the control of the tobacco Lhcb1*2 promoter, in etiolated transgenic tobacco seedlings. The activity of β-glucuronidase (GUS) showed biphasic responses to the calculated proportion of Pf ...
... We report here the synthesis and biological activity of a new persistent abscisic acid (ABA) analog, 8'-methylene ABA. This ABA analog has one additional carbon atom attached through a double bond to the 8'-carbon of the ABA molecule. (+)-8'-Methylene ABA is more active than the natural hormone (+)-ABA in inhibiting germination of cress seed and excised wheat embryos, in reducing growth of suspens ...
... The addition of abscisic acid (ABA) to mature non-dormant seeds inhibits their germination. This effect of ABA might be related to its natural function as an endogenous inhibitor of precocious germination during seed formation. In this work, we studied how ABA affects the germination of mature seeds and the growth of nascent seedlings of Arabidopsis thaliana (L.) Heynh. Our findings were as follow ...
... Described here are the first quantitative measurements of absolute amounts of mRNAs transcribed from individual members of a phytochrome gene (PHY) family. The abundances of PHY mRNAs were determined for dry seed and for selected organs of greenhouse-grown tomato (Solanum lycopersicum L.) seedlings and mature plants. With a PhosphorImager, absolute amounts of PHYA, PHYB1, PHYB2, PHYE and PHYF tran ...
... We hypothesized that photoinhibition of shade-developed leaves of deciduous hardwood saplings would limit their ability to acclimate photosynthetically to increased irradiance, and we predicted that shade-tolerant sugar maple (Acer saccharum Marsh.) would be more susceptible to photoinhibition than intermediately shade-tolerant red oak (Quercus rubra L.). After four weeks in a canopy gap, photosyn ...
protein synthesis; cell growth; hypocotyls; seedlings; Vigna radiata; amino acid sequences; epicotyls; protein content; glycoproteins
Abstract:
... Physiological changes were examined in the amount of a 50-kDa glycoprotein (gp50) that was recovered in a nuclear fraction from hypocotyls of mung bean (Vigna radiata) seedlings. Immunoblot analysis indicated that the glycoprotein was present in hypocotyls and epicotyls from 4- and 5-day-old seedlings but not in hypocotyls from 2-day-old seedlings. The glycoprotein was not detected in leaves or ro ...
net assimilation rate; Pinus ponderosa; acid deposition; ozone; gas exchange; tree age; seedlings; carbon dioxide; photosynthesis; stomatal conductance; water use efficiency; branches; pH; leaves; seasonal variation; genotype; California
... We determined that ribosomes of seedling roots of maize (Zea mays L.) contain the acidic phosphoproteins (P-proteins) known to form a flexible lateral stalk structure of the 60S subunit of eukaryotic ribosomes. The P-protein stalk, composed of P0, P1, and P2, interacts with elongation factors, mRNA, and tRNA during translation. Acidic proteins of 13 to 15.5 kD were released as a complex from ribos ...
Acacia tortilis; age determination; browsing; cambium; ecosystems; growth rings; regression analysis; seedlings; stems; Kenya
Abstract:
... Age determination on the basis of ring count holds great potential for the study of dry woodland ecosystem dynamics. Discs from stems of Acacia tortilis from Marsabit District, northern Kenya, were collected for dendrochronological analysis. Problems in the identification of growth rings are summarized. Regression analysis is used to develop age-ring count and ring count-size relationships. The ti ...
Picea rubens; Tomato mosaic virus; geographical distribution; seedlings; altitude; roots; disease prevalence; seasonal variation; sampling; enzyme-linked immunosorbent assay; New York; Vermont; New Hampshire; Maine
Abstract:
... To test the hypothesis that tomato mosaic Tobamovirus (ToMV) is transmitted by an airborne mechanism, virus-free red spruce (Picea rubens Sarg.) seedlings were planted in four raised, plywood boxes lined with plastic and filled with a Promix-sand (3:1, v/v) mixture. In July 1993, two boxes were placed at 1015 m (site 1) and two at 960 m (site 2) elevation on Whiteface Mountain, N.Y. ToMV was detec ...
Oryza sativa; seedlings; anaerobic conditions; roots; coleoptiles; cyclic GMP; cyclic AMP; quantitative analysis; chemical constituents of plants; stress response
Abstract:
... The imposition of anaerobic conditions resulted in a rapid (2 min) and transient increase in cGMP content in root and coleoptile of rice seedlings. The level of cAMP was strongly decreased by anoxia. These data are discussed in relation to the anaerobic response. ...
... Using dichromatic radiation, we show that the actions of phytochromes A and B (phyA and phyB) in Arabidopsis thaliana are antagonistic in mediating red and far-red radiation effects on seedling de-etiolation and yet act in a complementary manner to regulate de-etiolation, irrespective of spectral composition. At low phytochrome photoequilibria inhibition of hypocotyl extension was strong, because ...
... The apoplastic alpha-amylase (EC 3.2.1.1) in pea (Pisum sativum L. cv. Laxton's Progress No.9) seedlings was enhanced by a 4- to 6-fold with heat stress. Increased levels of the enzyme persisted for 48 h at least after the stress and subsequent heat treatments enhanced the activity further. These results indicate that the function of apoplastic alpha-amylase in vegetative tissues may be related to ...
... Many plant genes are responsive to sugars but the mechanisms used by plants to sense sugars are unknown. A genetic approach has been used in Arabidopsis to identify genes involved in perception and transduction of sugar signals. For this purpose, an in vivo reporter system was established consisting of the light- and sugar-regulated plastocyanin promoter, fused to the luciferase coding sequence (P ...
... To simulate the damage caused by herbivores and fungi and study their effects on 1-year-old nursery-grown sessile oak (Quercus petraea (Matt.) Liebl.) seedlings, (1) the portion of stem produced during the first growth flush, referred to as the growth unit (GU), was artificially defoliated, decapitated, or both or (2) the apical part of the GU was removed in the period of apparent rest. In compari ...
Avena sativa; seedlings; coleoptiles; phototropism; blue light; plant proteins; phosphorylation; protein synthesis; quantitative analysis; plasma membrane; etiolation
Abstract:
... The possible correlation between blue light-dependent phosphorylation of a 116-kD protein and phototropic responses of etiolated oat (Avena sativa L.) seedlings was tested by a micromethodfor protein phosphorylation. Quantitation of the basipetal distribution of this protein showed that the in vitro 32P phosphorylation values declined exponentially from tip to node, with more than 50% of the total ...
... Forest trees are major components of the terrestrial biome and their response to rising atmospheric CO2 plays a prominent role in the global carbon cycle. In this study, loblolly pine seedlings were planted in the field in recently disturbed soil of high fertility, and CO2 partial pressures were maintained at ambient CO2 (Amb) and elevated CO2 (Amb + 30 Pa) for 4 years. The objective of the study ...
... Xyloglucan, the primary hemicellulosic cell wall polysaccharide in dicotyledons, undergoes substantial modification during auxin-stimulated cell expansion. To identify candidates for mediating xyloglucan turnover, the expression and auxin regulation of tomato Cel7 and LeEXT, genes encoding an endo-1,4-beta-glucanase (EGase) and a xyloglucan endotransglycosylase (XET), respectively, were examined. ...
... A mutant of Arabidopsis that accumulates a high level of red pigments within the seed coat has been isolated from a population of T-DNA-transformed plants. Genetic analysis revealed that the mutation is recessive and affects maternal seed tissues only. Due to the color of the immature seeds, this mutation was named banyuls (ban). Pigments accumulated continuously from early seed development to the ...
... Twenty-five field trials conducted over a 3-year period in five San Joaquin Valley counties included the following treatments: nontreated cotton seed; seed treated with myclobutanil for the control of Rhizoctonia solani-induced damping-off; seed treated with metalaxyl for the control of Pythium-induced damping-off; and seed treated with a combination of the two fungicides. The following parameters ...
... The aim of this study was to identify the endophytic bacteria recovered from the foliage, tap roots and nodules of red clover plants (Trifolium pratense L.); and to assess the effects of the nodule bacteria, alone and in combination with Rhizobium spp., on the growth and development of red clover seedlings. Thirty-one bacteria species from 14 different genera were recovered from within the foliage ...
height; plant characteristics; Pisum sativum; seedlings; shoots; brassinosteroids; biosynthesis; quantitative analysis; chemical reactions; biochemical pathways; internodes; dose response; length; dwarfing; mutants; genetic variation; chemical constituents of plants; genotype; width
Abstract:
... Endogenous brassinosteroids (BRs) in the dwarf mutants Ika and Ikb of garden pea (Pisum sativum L.) and comparable wild-type plants were quantified by gas chromatography-selected ion monitoring using deuterated internal standards. In young shoots of the Ikb mutant, the levels of brassinolide, castasterone, and 6-deoxocastasterone were 23-, 22-, and 9-fold lower, respectively, than those of wild-ty ...
... Aboveground biomass allocation, and height and branch growth were studied in saplings of the shade-tolerant conifer, Abies amabilis Dougl. ex Forbes growing in large openings and in the understory of an old-growth forest in western Oregon. The presence of annual overwintering budscale scars was used to infer extension growth histories; annual growth rings in branches and stems were used in combina ...
Arabidopsis thaliana; seedlings; hypocotyls; ion transport; cytoplasm; calcium; dose response; blue light; cold stress; luminescence; plasma membrane
Abstract:
... The activation of an anion channel in the plasma membrane of Arabidopsis thaliana hypocotyls by blue light (BL) is believed to be a signal-transducing event leading to growth inhibition. Here we report that the open probability of this particular anion channel depends on cytoplasmic Ca2+ ([Ca2+]cyt) within the concentration range of 1 to 10 micrometers, raising the possibility that BL activates th ...
Arabidopsis thaliana; drought; salinity; water stress; cytosol; mannitol; seedlings; ion transport; chelating agents; lanthanum; gene expression; genes; ligases; verapamil; vacuoles; messenger RNA; inorganic ions; calcium; chemical constituents of plants; gadolinium; ethylene glycol tetraacetic acid
Abstract:
... Changes in cytosolic free calcium concentration ([Ca(2+)]cyt) in response to mannitol (drought) and salt treatments were detected in vivo in intact whole Arabidopsis seedlings. Transient elevations of [Ca(2+)]cyt to around 1.5 micromolars were observed, and these were substantially inhibited by pretreatment with the calcium-channel blocker lanthanum and to a lesser extent, the calcium-chelator EGT ...
... Agricultural chemical companies have invested in the discovery and development of biological pesticides to complement synthetic pesticides for the control of insects, diseases, and weeds on agronomic and horticultural crops. For plant disease control, companies envisage biological fungicides entering markets where they have the best chance of performing and which are most receptive to using biolog ...
Pinus ponderosa; seedlings; water stress; drought; drought tolerance; carbon; isotopes; gas exchange; net assimilation rate; photosynthesis; stomata; leaf conductance; water use efficiency; xylem water potential; chemical constituents of plants; stress response; South Dakota; Montana
Abstract:
... Effects of water supply on gas exchange, carbon isotopic composition, and relative growth rate were compared among seedlings from three populations of ponderosa pine (Pinus ponderosa Dougl. ex Laws.) grown in a controlled environment chamber. The three populations were chosen to represent high, moderate and low drought tolerance. There was no indication that drought tolerance was related to high w ...
Pinus ponderosa; seedlings; stems; conifer needles; ozone; phytotoxicity; roots; carbohydrates; quantitative analysis; biomass; chemical constituents of plants; stress response
Abstract:
... Ozone exposure decreases belowground carbon allocation and root growth of plants; however, the extent to which these effects persist and the cumulative impact of ozone stress on plant growth are poorly understood. To evaluate the potential for plant compensation, we followed the progression of ozone effects, with particular emphasis on the development of new roots. Ponderosa pine (Pinus ponderosa ...
... The potential role of antioxidant enzymes in protecting maize (Zea mays L.) seedlings from chilling injury was examined by analyzing enzyme activities and isozyme profiles of chilling-susceptible (CO 316) and chilling-tolerant (CO 328) inbreds. Leaf superoxide dismutase (SOD) activity in CO 316 was nearly one-half that of CO 328, in which the high activity was maintained during the chilling and po ...
... We studied the spatial distribution of fruits and plants, mortality and growth rates ofScaphium macropodum (Sterculiaceae) in four 1-ha plots in a tropical rain forest in West Kalimantan, Indonesia. The species is a large deciduous tree and produces wind-dispersed fruits on defoliated twigs. The density of dispersed fruits on the ground decreased with increasing distance from a parent tree. The ar ...
... Autolytic activities of coleoptile cell walls were measured in developing maize seedlings. The major neutral sugar components of the cell wall polysaccharides were arabinose, xylose and glucose. The quantities of all these components per coleoptile increased for 5 d after germination, suggesting that levels are augmented by biosynthetic processes during coleoptile growth. However, cell wall prepar ...
... Carob seedlings were grown hydroponically for 9 weeks under 360 and 800 microliters l-1 CO2. One of two nitrogen sources, nitrate or ammonium, was added to the nutrient medium at concentrations of 3 mol m-2. Root systems of the developing plants supplied with nitrate compared to those supplied with ammonium were characterized by: (a) more biomass on the lower part of the root; (b) fewer lateral ro ...
woodlands; natural regeneration; canopy; seedlings; stand density; understory; Quercus; Fraxinus excelsior; Betula pendula; England
Abstract:
... A survey of 78 sites in southern England with approved management plans for restocking by natural regeneration was made during the summers of 1993 and 1994. The following features were recorded: species, stocking, canopy cover and seed-bearing potential of trees present in the overstorey; species and canopy cover of the understorey; ground cover; species, browsing damage, number and heights of tre ...
restriction fragment length polymorphism; Picea engelmannii; hybrids; mitochondrial DNA; seedlings; chloroplast DNA; drought tolerance; gas exchange; chemotaxonomy; ecology; Picea glauca; frost resistance; artificial regeneration; genetic variation; plant physiology; Picea sitchensis; introgression; British Columbia
Abstract:
... The Nass Skeena Transition is an area located in British Columbia (BC), Canada, where a large introgression zone occurs between Sitka spruce (Picea sitchensis (Bong.) Carr.) and interior spruce (white spruce, Picea glauca (Moench) Voss x Engelmann spruce, Picea engelmannii Parry ex. Engelm.). When these species hybridize in this transition area, forestry programs face special problems in identifyi ...
... Salicylic acid (SA) plays an important signaling role in the resistance of many plants to pathogen invasion. Increases in endogenous SA levels have been associated with the hypersensitive response as well as systemic acquired resistance (SAR). SA also induces the expression of a subset of the pathogenesis-related (PR) genes. However, relatively little is known about the events occurring subsequent ...
Triticum aestivum; chloroplasts; dark adaptation; messenger RNA; mutation; operon; promoter regions; protein synthesis; seedlings; transcription (genetics); wheat
Abstract:
... Dynamical aspects of three chloroplast promoters responding to change in light condition were examined in mature chloroplasts of wheat (Triticum aestivum) by in vitro transcription. The wheat psbD/C operon has four distinct promoters, two of which named as D/C-3 and D/C-4 promoters dominantly function in mature chloroplasts to produce the mRNAs encoding D2/CP43 and CP43 alone, respectively. Activi ...
... As part of an ongoing investigation into the organization andregulation of the flavonoid biosynthetic pathway, two Arabidopsis thaliana expressed sequence tag (EST) clones (153O10T7 and YAY780) with high homology to leucoanthocyanidin dioxygenase (LDOX) or flavonol synthase (FLS) were identified. EST YAY780 was sequenced and found to encode a protein 49 to 78% identical to all LDOX sequences in th ...
... Phytochrome A (phyA) is a red/far-red (FR) light photoreceptor responsible for initiating numerous light-mediated plant growth and developmental responses, especially in FR light-enriched environments. We previously showed that the first 70 amino acids of the polypeptide contain at least two regions with potentially opposite functions (E.T. Jordan, J.R. Cherry, J.M. Walker, R.D. Vierstra [1996] Pl ...
... Uricase II is a purine metabolic enzyme highly induced in root nodules during the symbiosis established between legumes and bacteria of the genera Rhizobium and Bradyrhizobium. Here we describe the characterization of bean (Phaseolus vulgaris) nodule uricase II cDNA and show that uricase II is encoded by a single gene in the bean genome. This gene is also expressed in cotyledons, roots, and hypoco ...
... Chloropicrin has been tested as a soil fumigant in forest nurseries throughout the world. From 1944 to the late 1960's, tests were conducted in Australia, Canada, Netherlands, New Zealand, the United Kingdom and the United States. Since 1985, studies have been conducted in Georgia, Mississippi, South Carolina, Texas, Virginia, Washington and Wisconsin. In Wisconsin, chloropicrin reduced soilborne ...
... Cinnamate-4-hydroxylase (C4H) is the first Cyt P450-dependent monooxygenase of the phenylpropanoid pathway. To study the expression of this gene in Arabidopsis thaliana, a C4H cDNA clone from the Arabidopsis expressed sequence tag database was identified and used to isolate its corresponding genomic clone. The entire C4H coding sequence plus 2.9 kb of its promoter were isolated on a 5.4-kb HindIII ...
backcrossing; genetic resistance; seedlings; air temperature; frost resistance; latitude; provenance; Pinus sylvestris; seasonal variation; Finland
Abstract:
... The variation of timing of terminal bud set and frost hardening in first-year seedlings of Scots pine (Pinus sylvestris L.) was studied. Both bud set and frost hardiness showed clinal variation with respect to latitude over a steep environmental gradient (60-67 degrees N) in Finland. Northern populations set buds and developed frost hardiness earlier than southern populations. Backcrosses ((north ...
... Seedling regeneration and morphology of Oregon grape (Berberis nervosa Pursh) and salal (Gaultheria shallon Pursh) were studied in thinned and unthinned Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) stands in the central Coast Range, Oregon. Above- and below-ground growth of both species were significantly and negatively correlated with stand density. Oregon grape appears to have less potenti ...