Jump to Main Content
PubAg
Main content area
Search
Search Results
- Author:
- Orion, D.; Wergin, W.P.; Chitwood, D.J.; Erbe, E.F.
- Source:
- Journal of nematology 1994 v.26 no.4 pp. 402
- ISSN:
- 0022-300X
- Subject:
- Meloidogyne incognita; plant parasitic nematodes; ova; embryogenesis; ultrastructure
- Abstract:
- ... The root-knot nematode Meloidogyne incognita was cultured monoxenically on excised tomato roots. Galls and egg masses were observed daily using a light microscope. Two phases were distinguished in the gelatinous matrix of the egg mass: a translucent, amorphous material on the surface of the egg mass and a denser, layered phase in which nematode eggs were deposited. Egg masses were also cryofixed, ...
- Handle:
- 10113/7152
- PubMed:
- 19279909
- PubMed Central:
- PMC2619531
-
- Author:
- Asemota, O.; Breda, C.; Sallaud, C.; El Turk, J.; Kozak, I. de.; Buffard, D.; Esnault, R.; Kondorosi, A.
- Source:
- Plant molecular biology 1994 v.26 no.4 pp. 1201-1205
- ISSN:
- 0167-4412
- Subject:
- Medicago sativa; complementary DNA; ribosomes; proteins; nucleotide sequences; amino acid sequences; gene expression; messenger RNA
- Abstract:
- ... A cDNA encoding a putative cytoplasmic ribosomal protein LS from alfalfa (MsRL5), the first sequence from higher plants, has been characterized. The derived amino acid sequence of 181 residues contains the L5 signature, is 72.2% identical to yeast ribosomal L5 and shares high identity with other RL5 peptides from eukaryotic origin. The sequence does not contain any signal or transit peptide and th ...
- DOI:
- 10.1007/BF00040700
- PubMed:
- 7811977
-
http://dx.doi.org/10.1007/BF00040700
- Author:
- Fatimah, C.T.N.I.; Mutalib, A.R.; Shah Majid, M.
- Source:
- Tropical animal health and production 1994 v.26 no.4 pp. 247-250
- ISSN:
- 0049-4747
- Subject:
- Staphylococcus; sheep; keratoconjunctivitis; Bacillus (bacteria); Mycoplasma; Moraxella subgenus Branhamella; Streptococcus; conjunctiva; mixed infection; bacteria; Malaysia
- DOI:
- 10.1007/BF02240395
- PubMed:
- 7900224
-
http://dx.doi.org/10.1007/BF02240395
- Author:
- Payne, R.C.; Sukanto, I.P.; Partoutomo, S.; Jones, T.W.; Luckins, A.G.; Boid, R.
- Source:
- Tropical animal health and production 1994 v.26 no.4 pp. 219-226
- ISSN:
- 0049-4747
- Subject:
- calves; Trypanosoma evansi; antiprotozoal agents; subcutaneous injection; intramuscular injection; dose response; relapse; Holstein
- DOI:
- 10.1007/BF02240385
- PubMed:
- 7900218
-
http://dx.doi.org/10.1007/BF02240385
- Author:
- Roy, P.; Nachimuthu, K.; Venugopalan, A.T.; Dorairajan, N.; Purushothaman, V.; Koteeswaran, A.
- Source:
- Tropical animal health and production 1994 v.26 no.4 pp. 251-252
- ISSN:
- 0049-4747
- Subject:
- chickens; infectious bursal disease; Infectious bursal disease virus; sampling; filters; blood serum; antibody formation; accuracy
- DOI:
- 10.1007/BF02240397
- PubMed:
- 7900225
-
http://dx.doi.org/10.1007/BF02240397
- Author:
- Sangwan, A.K.; Chaudhri, S.S.; Sangwan, N.; Gupta, R.P.
- Source:
- Tropical animal health and production 1994 v.26 no.4 pp. 241-246
- ISSN:
- 0049-4747
- Subject:
- Hyalomma anatolicum anatolicum; Theileria; salivary glands; seasonal variation; ambient temperature; relative humidity; winter; summer; wet season; India
- DOI:
- 10.1007/BF02240394
- PubMed:
- 7900223
-
http://dx.doi.org/10.1007/BF02240394
- Author:
- Sanchez de la Hoz, P.; Castagnaro, A.; Carbonero, P.
- Source:
- Plant molecular biology 1994 v.26 no.4 pp. 1231-1236
- ISSN:
- 0167-4412
- Subject:
- messenger RNA; loci; complementary DNA; Hordeum vulgare; trypsin inhibitors; amino acid sequences; gene expression; endosperm; Triticum aestivum; nucleotide sequences; alpha-amylase
- Abstract:
- ... Amino acid sequences for three members (CMx1, CMx2, and CMx3) of a new subfamily of trypsin/alpha-amylase inhibitors in wheat have been deduced from the nucleotide sequences of the corresponding cDNAs. A cDNA clone encoding CMx1 was selected from a wheat developing endosperm library using a probe that encoded barley trypsin inhibitor BTI-CMe at low stringency. Sequences corresponding to CMx2 and C ...
- DOI:
- 10.1007/BF00040705
- PubMed:
- 7811982
-
http://dx.doi.org/10.1007/BF00040705
- Author:
- Marcinko-Kuehn, M.; Yang, X.; Debets, F.; Jacobson, D.J.; Griffiths, A.J.F.
- Source:
- Current genetics 1994 v.26 no.4 pp. 336-343
- ISSN:
- 0172-8083
- Subject:
- restriction mapping; Neurospora; mitochondrial DNA; DNA probes; plasmids; crossing; cytoplasmic inheritance; nucleic acid hybridization; Louisiana
- Abstract:
- ... Two Louisiana strains of Neurospora tetrasperma contain a linear plasmid (LA-kalDNA) with a restriction map identical to the Hawaiian Neurospora intermedia senescence plasmid, kalDNA, but with termini 100 nucleotide pairs shorter. One of these strains also bore a circular plasmid similar to the Hawaiian circular plasmid Hanalei-2. One species probably acquired both plasmids from the other by horiz ...
- DOI:
- 10.1007/BF00310498
- PubMed:
- 7882428
-
http://dx.doi.org/10.1007/BF00310498
- Author:
- Perysinakis, A.; Kinghorn, J.R.; Drainas, C.
- Source:
- Current genetics 1994 v.26 no.4 pp. 315-320
- ISSN:
- 0172-8083
- Subject:
- Saccharomycetales; nitrogen metabolism; alleles; loci; glutamate dehydrogenase; nutrient availability; NADP (coenzyme); ammonium compounds; mutants; enzyme activity; amino acid metabolism; glutamic acid
- Abstract:
- ... The initial velocity, pH and temperature optima, and Km values of Schizosaccharomyces pombe NADP-glutamate dehydrogenase (NADP-GDH: EC 1.4.1.4) have been determined. NADP-GDH was found to be specific for the substrates used in the reaction mixtures. NADP-GDH activity showed a sigmoidal response to changes in alpha-ketoglutarate concentrations, following Hill kinetics with a coefficient n(H) = 2. A ...
- DOI:
- 10.1007/BF00310495
- PubMed:
- 7882425
-
http://dx.doi.org/10.1007/BF00310495
- Author:
- Loeb, M.J.
- Source:
- Archives of insect biochemistry and physiology 1994 v.26 no.4 pp. 263-277
- ISSN:
- 0739-4462
- Subject:
- Heliothis virescens; testes; fat body; growth factors; ecdysterone; male genitalia; sexual development
- Abstract:
- ... Testis sheaths and fat body from developing male pupae of Heliothis virescens synthesize soluble growth factor-like products when exposed to 20-hydroxyecdysone (20HE). These factors promote growth and development of the genital tract. Saline extracts of modified Grace's medium and 20HE-treated testis sheaths and fat body were subjected to heat, freeze-thaw, organic solvents, and low pressure size ...
- DOI:
- 10.1002/arch.940260403
-
http://dx.doi.org/10.1002/arch.940260403
- Author:
- Rhounim, L.; Gregoire, A.; Salama, S.; Faugeron, G.
- Source:
- Current genetics 1994 v.26 no.4 pp. 344-351
- ISSN:
- 0172-8083
- Subject:
- Pezizales; genetic transformation; gene transfer; reporter genes; phosphotransferases (phosphomutases); crossing; gene expression; DNA methylation; cytosine; repetitive sequences; gene silencing
- Abstract:
- ... A large proportion of Ascobolus immersus transformants are highly unstable in crosses: the phenotype conferred by the transgene is not transmitted to the progeny, irrespective of the endogenous or foreign origin of the transgene. They all have integrated multiple transgene copies, clustered at a single chromosomal site or at tightly-linked sites. Clustered non-homologous integrations are always re ...
- DOI:
- 10.1007/BF00310499
- PubMed:
- 7882429
-
http://dx.doi.org/10.1007/BF00310499
- Author:
- Aleksenko, A.Y.
- Source:
- Current genetics 1994 v.26 no.4 pp. 352-358
- ISSN:
- 0172-8083
- Subject:
- genetic transformation; Aspergillus nidulans; plasmid vectors; homologous recombination
- Abstract:
- ... Transforming DNAs form cointegrates in Aspergillus nidulans by homologous and non-homologous recombination as well as by end-to-end ligation of linear fragments. This process has been studied by means of a model in which the linkage of a marker gene to the origin of autonomous replication AMA1 was selected for. Recombinant plasmids were rescued into Escherichia coli and subjected to restriction ma ...
- DOI:
- 10.1007/BF00310500
- PubMed:
- 7882430
-
http://dx.doi.org/10.1007/BF00310500
- Author:
- Touhara, K.; Wojtasek, H.; Prestwich, G.D.
- Source:
- Archives of insect biochemistry and physiology 1994 v.26 no.4 pp. 287-297
- ISSN:
- 0739-4462
- Subject:
- Manduca sexta; chemical reactions; farnesol; binding proteins; hemolymph; binding sites; juvenile hormones
- Abstract:
- ... The 32 kD juvenile hormone binding protein (JHBP) and two 80 kD proteins in larval Manduca sexta hemolymph were labeled with [3H]FDK, a photoaffinity analog of methyl farnesoate (MF). The labeling could be completely displaced by a 30-fold excess of either MF or JH II, demonstrating that [3H]FDK binds specifically to the JH binding sites of the 32 kD JHBP and the 80 kD proteins. In addition, a hig ...
- DOI:
- 10.1002/arch.940260405
-
http://dx.doi.org/10.1002/arch.940260405
- Author:
- Ferreira, C.; Capella, A.N.; Sitnik, R.; Terra, W.R.
- Source:
- Archives of insect biochemistry and physiology 1994 v.26 no.4 pp. 299-313
- ISSN:
- 0739-4462
- Subject:
- glycosidases; proteinases; midgut; microvilli; Spodoptera frugiperda; digestion
- Abstract:
- ... In the midgut of Spodoptera frugiperda larvae, subcellular fractionation data suggest that aminopeptidase and part of amylase, carboxypeptidase A, dipeptidase, and trypsin are bound to the microvillar membranes; that major amounts of soluble dipeptidase, cellobiase, and maltase are trapped in the cell glycocalyx; and finally that soluble carboxypeptidase, amylase, and trypsin occur in intracellula ...
- DOI:
- 10.1002/arch.940260406
-
http://dx.doi.org/10.1002/arch.940260406
- Author:
- Hamelin, R.C.; Doudrick, R.L.; Nance, W.L.
- Source:
- Current genetics 1994 v.26 no.4 pp. 359-363
- ISSN:
- 0172-8083
- Subject:
- Cronartium fusiforme; genetic markers; geographical variation; genetic variation; rust diseases; Pinus taeda; random amplified polymorphic DNA technique; Southeastern United States
- Abstract:
- ... Twelve random amplified polymorphic DNA (RAPD) markers were used to study genetic variability in three populations of Cronartium quercuum f. sp. fusiforme, the causal agent of fusiform rust on pines. Most of the variability (94%) was found within populations while 6% of the total variability was attributable to frequency differences between populations. The frequency of three markers was significa ...
- DOI:
- 10.1007/BF00310501
- PubMed:
- 7882431
-
http://dx.doi.org/10.1007/BF00310501
- Author:
- Gosalbo, L.; Fabrias, G.; Camps, F.
- Source:
- Archives of insect biochemistry and physiology 1994 v.26 no.4 pp. 279-286
- ISSN:
- 0739-4462
- Subject:
- Spodoptera littoralis; stearoyl-CoA desaturase; enzyme activity; cyclopropenoid fatty acids; sex pheromones; biosynthesis; unsaturated fatty acids
- Abstract:
- ... The effect of 10,11-methylenetetradec-10-enoic acid on the sex pheromone biosynthetic pathway of Spodoptera littoralis is reported. This new cyclopropene fatty acid inhibited the biosynthesis of the main pheromone component from labeled myristic acid. The study of each Z desaturation step revealed that the Z9-desaturase of E11-14:Acid was inhibited, whereas the Z11-desaturase of 16:Acid was not af ...
- DOI:
- 10.1002/arch.940260404
-
http://dx.doi.org/10.1002/arch.940260404
- Author:
- Esposito, M.S.; Ramirez, R.M.; Bruschi, C.V.
- Source:
- Current genetics 1994 v.26 no.4 pp. 302-307
- ISSN:
- 0172-8083
- Subject:
- mitosis; nitrogen metabolism; mitotic recombination; diploidy; mutation; Saccharomyces cerevisiae; heterozygosity; dicarboxylic acids; recessive genes
- Abstract:
- ... We have employed the analysis of spontaneous forward mutations that confer the ability to utilize L-alpha-aminoadipate as a nitrogen source (alpha-Aa+) to discern the events that contribute to mitotic segregation of spontaneous recessive mutations by diploid cells. alpha-Aa- diploid cells yield alpha-Aa+ mutants at a rate of 7.8 +/- 3.6 X 10(-9). As in haploid strains, approximately 97% (30/31) of ...
- DOI:
- 10.1007/BF00310493
- PubMed:
- 7882423
-
http://dx.doi.org/10.1007/BF00310493
- Author:
- Chong, D.K.X.; Yang, R.C.; Yeh, F.C.
- Source:
- Current genetics 1994 v.26 no.4 pp. 374-376
- ISSN:
- 0172-8083
- Subject:
- Populus tremuloides; genetic markers; genetic variation; geographical variation; loci; gene frequency; random amplified polymorphic DNA technique; Alberta
- Abstract:
- ... In the present study, a total of 142 trees sampled from five populations of trembling aspen (Populus tremuloides Michx.) in Alberta was analyzed by the polymerase chain reaction (PCR) with five random oligonucleotide primers. Null-allele frequencies of 28 putative RAPD loci were estimated using the given departure from Hardy-Weinberg equilibrium (F(IS)) previously estimated with isozyme markers fo ...
- DOI:
- 10.1007/BF00310504
- PubMed:
- 7882434
-
http://dx.doi.org/10.1007/BF00310504
- Author:
- Escriche, B.; Martinez-Ramirez, A.C.; Real, M.D.; Silva, F.J.; Ferre, J.
- Source:
- Archives of insect biochemistry and physiology 1994 v.26 no.4 pp. 315-327
- ISSN:
- 0739-4462
- Subject:
- Phthorimaea operculella; Bacillus thuringiensis; bacterial toxins; endotoxins; receptors; cell membranes; midgut; bacterial insecticides; binding sites
- Abstract:
- ... The potato tuber moth is susceptible to at least three insecticidal crystal proteins (ICPs) from Bacillus thuringiensis: CryIA(b), CryIB, and CryIC. To design useful combinations of toxin genes either in transgenic plants or in new genetically modified B. thuringiensis strains, it is necessary to determine the binding characteristics of the different ICPs so as not to combine a pair sharing the sa ...
- DOI:
- 10.1002/arch.940260407
-
http://dx.doi.org/10.1002/arch.940260407
- Author:
- Baines, D.; Downer, R.G.H.
- Source:
- Archives of insect biochemistry and physiology 1994 v.26 no.4 pp. 249-261
- ISSN:
- 0739-4462
- Subject:
- Periplaneta americana; hemocytes; octopamine; serotonin; inositol phosphates; phagocytosis; receptors
- Abstract:
- ... Octopamine and 5-hydroxytryptamine (5-HT) were previously/shown to affect phagocytosis in cockroach hemocytes through unidentified receptor-mediated events. In the present study, we examined the ability of 5-HT and octopamine to enhance inositol trisphosphate (IP3) production using hemocyte membranes of the American cockroach, Periplaneta americana. Octopamine enhanced IP3 production with a maxima ...
- DOI:
- 10.1002/arch.940260402
- PubMed:
- 8068962
-
http://dx.doi.org/10.1002/arch.940260402
- Author:
- Castillo, A.; Cifuentes, V.
- Source:
- Current genetics 1994 v.26 no.4 pp. 364-368
- ISSN:
- 0172-8083
- Subject:
- RNA; Deuteromycotina; cytotoxicity
- Abstract:
- ... Four double-stranded RNA (dsRNA) molecules were isolated from Phaffia rhodozyma UCD 67-385. Their molecular sizes were approximately 4.3, 3.1, 0.9 and 0.75 kilobase pairs (kbp) as determined by agarose-gel electrophoresis and they were designated as L, M, S1 and S2, respectively. By differential centrifugation in sucrose gradients, these dsRNAs copurified with isometric virus-like particles 36 nm ...
- DOI:
- 10.1007/BF00310502
- PubMed:
- 7882432
-
http://dx.doi.org/10.1007/BF00310502
- Author:
- Hirata, D.; Yano, K.; Miyahara, K.; Mijakawa, T.
- Source:
- Current genetics 1994 v.26 no.4 pp. 285-294
- ISSN:
- 0172-8083
- Subject:
- transport proteins; structural genes; drugs; Saccharomyces cerevisiae; mutagenicity; amino acid sequences; nucleotide sequences; plasma membrane; glycoproteins; drug resistance
- Abstract:
- ... A multidrug resistance gene, YDR1, of Saccharomyces cerevisiae, which encodes a 170-kDa protein of a member of the ABC superfamily, was identified. Disruption of YDR1 resulted in hypersensitivity to cycloheximide, cerulenin, compactin, staurosporine and fluphenazine, indicating that YDR1 is an important determinant of cross resistance to apparently-unrelated drugs. The Ydr1 protein bears the highe ...
- DOI:
- 10.1007/BF00310491
- PubMed:
- 7882421
-
http://dx.doi.org/10.1007/BF00310491
- Author:
- Hohmann, S.; Dijck, P. van.; Luyten, K.; Thevelein, J.M.
- Source:
- Current genetics 1994 v.26 no.4 pp. 295-301
- ISSN:
- 0172-8083
- Subject:
- hexosyltransferases; carbohydrate metabolism; fermentation; alleles; structural genes; glutamine; transfer RNA; Saccharomyces cerevisiae; trehalose; nucleotide sequences; fructose
- Abstract:
- ... Byp1-3 is an amber nonsense allele of the Saccharomyces cerevisiae GGS1/TPS1 gene which encodes the small subunit of the trehalose synthase complex. Mutations in this gene confer an inability to grow on glucose or fructose but the phenotype of byp1-3 mutants is leaky in a strain-dependent manner. Overexpression of the isolated byp1-3 allele suppressed the growth defect of a ggS1/tps1 delta mutant. ...
- DOI:
- 10.1007/BF00310492
-
http://dx.doi.org/10.1007/BF00310492
- Author:
- Ragnini, A.; Teply, R.; Waldherr, M.; Voskova, A.; Schweyen, R.J.
- Source:
- Current genetics 1994 v.26 no.4 pp. 308-314
- ISSN:
- 0172-8083
- Subject:
- metabolism; structural genes; guanosine triphosphate; G-proteins; Saccharomyces cerevisiae; amino acid sequences; mitochondria; nucleotide sequences
- Abstract:
- ... MRS6 is a newly-identified gene in the yeast Saccharomyces cerevisiae. Its product Mrs6p shows significant homology to the mammalian GDP dissociation inhibitor (GDI) of Rab/Ypt-type small G proteins and to the human choroideraemia protein (CHM), the component A of Rab-specific GGTase II. The interaction of Mrs6p with G proteins is indicated by our observation that the MRS6 gene suppresses the effe ...
- DOI:
- 10.1007/BF00310494
- PubMed:
- 7882424
-
http://dx.doi.org/10.1007/BF00310494
- Author:
- Gunge, N.; Takahashi, S.; Fukuda, K.; Ohnishi, T.; Meinhardt, F.
- Source:
- Current genetics 1994 v.26 no.4 pp. 369-373
- ISSN:
- 0172-8083
- Subject:
- Saccharomyces cerevisiae; Kluyveromyces marxianus var. lactis; Debaryomyces hansenii; plasmids; ultraviolet radiation; DNA repair
- Abstract:
- ... The Kluyveromyces linear plasmids pGKL1 and pGKL2, encoding killer activity, were efficiently cured by UV irradiation. This event was investigated in more detail by the use of the terminal protein (TP)-associated cytoplasmic linear plasmids, pJKL1 and pRKL2, with a selectable marker LEU2. This observation was compared with the UV effect on the nuclear plasmids pLS1 (telomere-associated linear form ...
- DOI:
- 10.1007/BF00310503
- PubMed:
- 7882433
-
http://dx.doi.org/10.1007/BF00310503