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- Author:
- Mu Luo; Lin Zhou; Shi-jie Zhan; Li-juan Cheng; Ruo-nan Li; Hui wang; Bin Liu; Hua Linghu
- Source:
- Biochemical and biophysical research communications 2019 v.513 no.2 pp. 528-533
- ISSN:
- 0006-291X
- Subject:
- animal ovaries; cell lines; cell movement; gene ontology; gene overexpression; genes; genetic disorders; histology; ligands; neoplasm cells; ovarian neoplasms; patients; small interfering RNA
- Abstract:
- ... The ALPL gene is linked to hypophosphatasia, a rare genetic disease. Owing to the inverse relationships between ALPL expression and both the International Federation of Gynecology and Obstetrics (FIGO) stages and histological grades assigned to patients with serous ovarian cancer (SOC), this study was designed to explore the role and possible mechanisms of ALPL in cell motility of high grade SOC ( ...
- DOI:
- 10.1016/j.bbrc.2019.04.016
- https://doi.org/10.1016/j.bbrc.2019.04.016
- Author:
- Kohei Kaneyoshi; Keiji Uchiyama; Masayoshi Onitsuka; Noriko Yamano; Yuichi Koga; Takeshi Omasa
- Source:
- Journal of bioscience and bioengineering 2019 v.127 no.1 pp. 107-113
- ISSN:
- 1389-1723
- Subject:
- Chinese hamsters; animal ovaries; antibodies; antibody formation; biopharmaceuticals; cell culture; cell lines; endoplasmic reticulum; engineering; gel chromatography; immunoglobulin G; microscopy; secretion
- Abstract:
- ... The production of biopharmaceutical immunoglobulin G (IgG) using cultured mammalian cells, especially Chinese hamster ovary (CHO) cells is well established and has been markedly improved through the modification of cells and cell culture engineering technologies. The establishment of high-production cell lines remains a challenge. The intracellular secretion of IgG has been investigated to identif ...
- DOI:
- 10.1016/j.jbiosc.2018.06.018
- https://doi.org/10.1016/j.jbiosc.2018.06.018
- Author:
- Christian Seca; Alessandra Ferraresi; Suratchanee Phadngam; Chiara Vidoni; Ciro Isidoro
- Source:
- Journal of materials chemistry B 2019 v.7 no.35 pp. 5376-5391
- ISSN:
- 2050-7518
- Subject:
- adverse effects; animal ovaries; autophagy; cell lines; chemistry; fluorescence; genes; genetic background; mitochondria; nanoparticles; neoplasm cells; ovarian neoplasms; polystyrenes; proteins; risk; stress response; therapeutics; toxicity
- Abstract:
- ... The use of nanoparticles (NPs) for diagnostic and therapeutic purposes involves the risk of side effects due to the presence of reactive groups on their surface. We studied the cellular stress response to spheroid fluorescent polystyrene nanoparticles (PS-NPs) functionalized with Amino groups in two ovarian cancer cell lines differing in the expression, among others, of relevant proteins involved ...
- DOI:
- 10.1039/c9tb00935c
- https://doi.org/10.1039/c9tb00935c
- Author:
- Sara Pereira; Daniel Ley; Mikkel Schubert; Lise Marie Grav; Helene Faustrup Kildegaard; Mikael Rørdam Andersen
- Source:
- Journal of biotechnology 2019 v.306 pp. 24-31
- ISSN:
- 0168-1656
- Subject:
- Chinese hamsters; animal ovaries; branched chain amino acids; byproducts; cell growth; cell lines; essential amino acids; genes; isoleucine; leucine; metabolites; product quality; protein synthesis; recombinant proteins; toxic substances; transferases; valine
- Abstract:
- ... In recombinant protein expression using Chinese hamster ovary (CHO) cells, chemically defined media contain essential amino acids such as branched chain amino acids (BCAAs) leucine, isoleucine and valine. Availability of amino acids is critical as these are building blocks for protein synthesis. However, breakdown of amino acids can lead to build up of toxic intermediates and metabolites that decr ...
- DOI:
- 10.1016/j.jbiotec.2019.08.017
- https://doi.org/10.1016/j.jbiotec.2019.08.017
- Author:
- Haiqin SongThe two authors contributed equally to this work.; Feifei Quan; Zhiqiang Yu; Minhua Zheng; Yan Ma; Haihua Xiao; Fang Ding
- Source:
- Journal of materials chemistry B 2019 v.7 no.3 pp. 433-442
- ISSN:
- 2050-7518
- Subject:
- adverse effects; animal ovaries; antineoplastic activity; cell lines; chemistry; cisplatin; cytotoxicity; drug resistance; endocytosis; in vivo studies; iron oxides; magnetic fields; nanocarriers; nanoparticles; neoplasm cells; ovarian neoplasms; platinum; toxicity testing; zeta potential
- Abstract:
- ... Platinum (Pt)-based drugs including cisplatin, carboplatin and oxaliplatin have been widely used as first-line anticancer regimens due to their excellent anticancer efficacy. However, the clinical application of these drugs is greatly limited due to their side effects and drug resistance. In this study, an antitumor drug delivery system based on carboplatin prodrug loading Fe₃O₄ nanoparticles (NPs ...
- DOI:
- 10.1039/c8tb02574f
- https://doi.org/10.1039/c8tb02574f
- Author:
- Danzi Song; Jiwei Cui; Yi Ju; Matthew Faria; Huanli Sun; Christopher B. Howard; Kristofer J. Thurecht; Frank Caruso
- Source:
- ACS applied materials & interfaces 2019 v.11 no.32 pp. 28720-28731
- ISSN:
- 1944-8252
- Subject:
- Chinese hamsters; animal ovaries; antibodies; breast neoplasms; cell lines; dosimetry; epidermal growth factor receptors; humans; macrophages; mice; mixing; neoplasm cells; polyethylene glycol; surface area
- Abstract:
- ... In the present study, a capsule system that consists of a stealth carrier based on poly(ethylene glycol) (PEG) and functionalized with bispecific antibodies (BsAbs) is introduced to examine the influence of the capsule shape and size on cellular targeting. Hollow spherical and rod-shaped PEG capsules with tunable aspect ratios (ARs) of 1, 7, and 18 were synthesized and subsequently functionalized ...
- DOI:
- 10.1021/acsami.9b10304
- https://doi.org/10.1021/acsami.9b10304
- Author:
- Rossina Domínguez-Ríos; Dante R. Sánchez-Ramírez; Kassandra Ruiz-Saray; Paola E. Oceguera-Basurto; Mario Almada; Josué Juárez; Abraham Zepeda-Moreno; Alicia del Toro-Arreola; Antonio Topete; Adrián Daneri-Navarro
- Source:
- Colloids and surfaces 2019 v.178 pp. 199-207
- ISSN:
- 0927-7765
- Subject:
- animal ovaries; breast neoplasms; cell lines; cisplatin; clinical trials; cytotoxicity; drug therapy; epithelium; fluorescein; fluorescence; genetic markers; monoclonal antibodies; multiple drug resistance; nanoparticles; neoplasm cells; ovarian neoplasms; patients; receptors; resistance mechanisms; taxanes; zeta potential
- Abstract:
- ... The conventional treatment (cytoreduction combined with cisplatin/carboplatin and taxane drugs) of ovarian cancer has a high rate of failure and recurrence despite a favorable initial response. This lack of success is usually attributed to the development of multidrug resistance mechanisms by cancer cells and avoidance of the anti-growth effects of monoclonal targeted therapeutic antibodies. The d ...
- DOI:
- 10.1016/j.colsurfb.2019.03.011
- https://doi.org/10.1016/j.colsurfb.2019.03.011
8. Comparing two automated high throughput viable-cell counting systems for cell culture applications
- Author:
- Séverine Fagète; Cédric Steimer; Pierre-Alain Girod
- Source:
- Journal of biotechnology 2019 v.305 pp. 23-26
- ISSN:
- 0168-1656
- Subject:
- Chinese hamsters; animal ovaries; automation; biopharmaceuticals; business enterprises; cell culture; cell lines; cell viability; counters; labor; monitoring
- Abstract:
- ... Cell counting and viability assessment is an integral part of mammalian cell line development. While manual counting with a hemocytometer is still the gold standard method, its subjectivity and high labor intensity has resulted in its reduced use in favor of automated systems. In addition, some of these automated systems offer multiwell plate based high throughput cell count, which is an asset for ...
- DOI:
- 10.1016/j.jbiotec.2019.08.014
- https://doi.org/10.1016/j.jbiotec.2019.08.014
- Author:
- Hamideh Parhiz; Stephanie A. Ketcham; Guozhang Zou; Bidesh Ghosh; Erica J. Fratz-Berilla; Muhammad Ashraf; Tongzhong Ju; Chikkathur N. Madhavarao
- Source:
- Applied microbiology and biotechnology 2019 v.103 no.15 pp. 6081-6095
- ISSN:
- 0175-7598
- Subject:
- Chinese hamsters; animal ovaries; beta-glucuronidase; bioreactors; butyrates; capillary electrophoresis; cell lines; drugs; enzyme replacement therapy; matrix-assisted laser desorption-ionization mass spectrometry; molecular models; pH; protein synthesis; purification methods; temperature
- Abstract:
- ... β-Glucuronidase is a lysosomal enzyme and a molecular model of a class of therapeutics approved as enzyme replacement therapies for lysosomal storage diseases. Understanding the effect of bioreactor process variables on the production and quality of the biologics is critical for maintaining quality and efficacy of the biotherapeutics. Here, we have investigated the effect of three process variable ...
- DOI:
- 10.1007/s00253-019-09889-7
- https://doi.org/10.1007/s00253-019-09889-7
- Author:
- Najmeh Jooyan; Bahram Goliaei; Bahareh Bigdeli; Reza Faraji-Dana; Ali Zamani; Milad Entezami; Seyed Mohammad Javad Mortazavi
- Source:
- Environmental research 2019 v.174 pp. 176-187
- ISSN:
- 0013-9351
- Subject:
- DNA damage; absorption; adverse effects; animal ovaries; cell death; cell lines; culture media; direct contact; electromagnetic field; energy; hamsters; ionizing radiation; membrane permeability; mitosis; mobile telephones; olives; radio waves; reactive oxygen species
- Abstract:
- ... The rapid rise in global concerns about the adverse health effects of exposure to radiofrequency radiation (RFR) generated by common devices such as mobile phones has prompted scientists to further investigate the biological effects of these environmental exposures. Non-targeted effects (NTEs) are responses which do not need a direct exposure to be expressed and are particularly significant at low ...
- DOI:
- 10.1016/j.envres.2019.03.063
- https://doi.org/10.1016/j.envres.2019.03.063
- Author:
- Bhuvana S. Doddapaneni; Adel M. AL-Fatease; Deepa A. Rao; Adam W.G. Alani
- Source:
- Journal of controlled release 2019 v.307 pp. 272-281
- ISSN:
- 0168-3659
- Subject:
- anaerobic conditions; angiogenesis; animal models; animal ovaries; apoptosis; cell lines; composite polymers; drug resistance; drugs; evaporation; hypoxia; immunohistochemistry; in vitro studies; mammals; micelles; mutation; ovarian neoplasms; phenotype; polyethylene glycol; rapamycin; remission; signal transduction; solubility; solvents; therapeutics
- Abstract:
- ... Mutations in the tumor protein (TP53) and the mammalian target of rapamycin (mTOR) pathway have been elucidated as driver mutations in ovarian carcinomas that transform into an invasive phenotype under hypoxic conditions. Chetomin (CHE) targets the hypoxic pathway while Everolimus (EVR) acts on the mTOR pathway. Poor aqueous solubilities of both compounds limit their clinical applications. Diblock ...
- DOI:
- 10.1016/j.jconrel.2019.06.036
- https://doi.org/10.1016/j.jconrel.2019.06.036
- Author:
- Jidapa Yamkasem; Puntanat Tattiyapong; Attapon Kamlangdee; Win Surachetpong
- Source:
- Journal of fish diseases 2019 v.42 no.9 pp. 1293-1300
- ISSN:
- 0140-7775
- Subject:
- RNA; Tilapia lake virus; animal ovaries; biosecurity; breeding stock; cell lines; fish diseases; fish fry; genomics; hatcheries; in situ hybridization; liver; progeny; testes; virus transmission; viruses
- Abstract:
- ... Tilapia lake virus disease (TiLVD) is an emerging viral disease in tilapia with worldwide distribution. Although the horizontal transmission of TiLV has been demonstrated through the cohabitation of infected fish with susceptible fish, no direct experiment showed the potential of vertical transmission from broodstock to progeny. In this study, natural outbreaks of TiLV in broodstock and fry in two ...
- DOI:
- 10.1111/jfd.13050
- https://doi.org/10.1111/jfd.13050
- Author:
- Aydan Dag; Pinar Sinem Omurtag Ozgen; Sezen Atasoy
- Source:
- Biomacromolecules 2019 v.20 no.8 pp. 2962-2972
- ISSN:
- 1526-4602
- Subject:
- Chinese hamsters; adverse effects; animal ovaries; antineoplastic agents; breast neoplasms; cell lines; composite polymers; cytotoxicity; drug therapy; glycosylation; hydrophobicity; kidney neoplasms; light scattering; moieties; nanocarriers; nanoparticles; neoplasm cells; platinum; polymerization; prostatic neoplasms; toxicity testing; transmission electron microscopy
- Abstract:
- ... An important requirement to decrease the side effects of chemotherapy drugs is to develop nanocarriers with precise biological functions. In this work, a set of glyconanoparticles was prepared via self-assembly of amphiphilic glycoblock copolymers for the targeted delivery of a hydrophobic chemotherapy drug. Well-defined glycoblock copolymers that consist of 1,1-di-tert-butyl 3-(2-(metyloyloxy)eth ...
- DOI:
- 10.1021/acs.biomac.9b00528
- https://doi.org/10.1021/acs.biomac.9b00528
- Author:
- Kaiming Chen; Dong Li; Hongwen Li; Bing Li; Ruiqin Wang; Lingxiao Jiang; Lei Huang; Xiaoqing Xu; Jie Li; Fei Teng; Cizhong Jiang; Hua Gu; Jianmin Fang
- Source:
- Biotechnology letters 2019 v.41 no.6-7 pp. 719-732
- ISSN:
- 0141-5492
- Subject:
- Chinese hamsters; RNA; animal ovaries; cell lines; gene expression regulation; genes; protein synthesis; protein transport; recombinant proteins; sequence analysis; transcription (genetics); transcriptome
- Abstract:
- ... OBJECTIVES: To identify genes that affected protein expression in Chinese hamster ovary (CHO) cells was significant, and we identified the changes in the transcriptome and the functional gene sets that would contribute to increase expression of recombinant protein. RESULTS: Here two sub-clones from a methotrexate-treated parental recombinant CHO cell line were selected. The two sub-clones, with di ...
- DOI:
- 10.1007/s10529-019-02681-7
- https://doi.org/10.1007/s10529-019-02681-7
- Author:
- Che Lin Kim; Gyun Min Lee
- Source:
- Metabolic engineering 2019 v.52 pp. 57-67
- ISSN:
- 1096-7176
- Subject:
- Chinese hamsters; animal ovaries; autocrine signaling; bone morphogenetic proteins; cell lines; clones; gene editing; gene expression; genes; growth retardation; humans; messenger RNA; methotrexate; signal transduction
- Abstract:
- ... A Chinese hamster ovary (CHO) cell line producing recombinant human bone morphogenetic protein-4 (rhBMP-4) (CHO-BMP-4), which expresses essential components of BMP signal transduction, underwent autocrine BMP-4 signaling. RNA seq analysis on CHO host cells (DG44) treated with rhBMP-4 (20 µg/mL) suggested that rhBMP-4 induced signaling in CHO cells could be a critical factor in limiting rhBMP-4 pro ...
- DOI:
- 10.1016/j.ymben.2018.11.003
- https://doi.org/10.1016/j.ymben.2018.11.003
- Author:
- Katarina Michalcova; Shubhadeep Roychoudhury; Marek Halenar; Eva Tvrda; Eva Kovacikova; Jaromir Vasicek; Peter Chrenek; Simona Baldovska; Luboslav Sanislo; Vladimir Kren; Adriana Kolesarova
- Source:
- Journal of environmental science and health 2019 v.54 no.9 pp. 752-757
- ISSN:
- 1532-4109
- Subject:
- animal ovaries; apoptosis; cell lines; cell viability; chemiluminescence; dietary supplements; enzyme-linked immunosorbent assay; flow cytometry; isoquercitrin; neoplasm cells; ovarian neoplasms; oxidative stress; reactive oxygen species; transforming growth factor beta 1
- Abstract:
- ... Isoquercitrin is a dietary bioflavonoid used as a food supplement. We studied the mechanism underlying its effect in human ovarian cancer cells using OVCAR-3 cell line. Viability, survival, apoptosis, release of human transforming growth factor-β1 (TGF-β1) and TGF-β1 receptor, and intracellular reactive oxygen species (ROS) generation by OVCAR-3 cells were examined after isoquercitrin treatment at ...
- DOI:
- 10.1080/03601234.2019.1633214
- https://doi.org/10.1080/03601234.2019.1633214
- Author:
- Junqing He; Junyan Wang; Nan Zhang; Luyao Shen; Linlin Wang; Xiao Xiao; Yan Wang; Tao Bing; Xiangjun Liu; Songqing Li; Dihua Shangguan
- Source:
- Talanta 2019 v.194 pp. 437-445
- ISSN:
- 0039-9140
- Subject:
- animal ovaries; blood serum; cell lines; dissociation; drug resistance; glycoproteins; humans; ions; ligands; neoplasm cells; nucleases; nucleotide aptamers; ovarian neoplasms; prognosis; survival rate; temperature; therapeutics
- Abstract:
- ... Ovarian cancer is regarded as the most lethal gynecologic malignancy with poor prognosis and high mortality rate. Drug-resistance was thought to be the main obstacle to improving overall survival rate of ovarian cancer. New ligands for drug-resistant ovarian cancer cells have potential for the development of diagnosis and therapy of ovarian cancer. In present work, we reported two aptamers, HF3–58 ...
- DOI:
- 10.1016/j.talanta.2018.10.028
- https://doi.org/10.1016/j.talanta.2018.10.028
- Author:
- Orla Coleman; Srinivas Suda; Justine Meiller; Michael Henry; Markus Riedl; Niall Barron; Martin Clynes; Paula Meleady
- Source:
- Journal of proteomics 2019 v.195 pp. 23-32
- ISSN:
- 1874-3919
- Subject:
- animal ovaries; biogenesis; biopharmaceuticals; cell growth; cell lines; engineering; hamsters; immunoglobulin G; liquid chromatography; microRNA; phenotype; protein synthesis; proteome; proteomics; recombinant proteins; ribosomes; tandem mass spectrometry
- Abstract:
- ... Cell line engineering using microRNAs represents a desirable route for improving the efficiency of recombinant protein production by CHO cells. In this study we generated stable CHO DP12 cells expressing a miR-7 sponge transcript which sequesters miR-7 from its endogenous targets. Depletion of miR-7 results in a 65% increase in cell growth and >3-fold increase in yield of secreted IgG protein. Qua ...
- DOI:
- 10.1016/j.jprot.2019.01.003
- https://doi.org/10.1016/j.jprot.2019.01.003
- Author:
- Michael Kleemann; Helga Schneider; Kristian Unger; Jeremias Bereuther; Simon Fischer; Philip Sander; E. Marion Schneider; Pamela Fischer-Posovszky; Christian U. Riedel; René Handrick; Kerstin Otte
- Source:
- Cellular and molecular life sciences 2019 v.76 no.3 pp. 539-559
- ISSN:
- 1420-682X
- Subject:
- DNA; animal ovaries; apoptosis; caspases; cell lines; gene expression regulation; luciferase; membrane potential; messenger RNA; microRNA; mitochondrial membrane; neoplasm cells; ovarian neoplasms; phosphorylation; protein synthesis; protein-serine-threonine kinases; proto-oncogenes; serine; signal transduction; threonine; transcription (genetics); transfection
- Abstract:
- ... Apoptosis is a form of directed programmed cell death with a tightly regulated signalling cascade for the destruction of single cells. MicroRNAs (miRNAs) play an important role as fine tuners in the regulation of apoptotic processes. MiR-493-3p mimic transfection leads to the induction of apoptosis causing the breakdown of mitochondrial membrane potential and the activation of Caspases resulting i ...
- DOI:
- 10.1007/s00018-018-2958-x
- https://doi.org/10.1007/s00018-018-2958-x
- Author:
- Dongya Zhang; Hongjun Guo; Wei Feng; Haifeng Qiu
- Source:
- Life sciences 2019 v.232 pp. 116648
- ISSN:
- 0024-3205
- Subject:
- Western blotting; animal ovaries; apoptosis; bioinformatics; carcinogenesis; cell lines; cell proliferation; flow cytometry; gene expression regulation; gene overexpression; laminin; luciferase; mitogen-activated protein kinase; neoplasm cells; neoplasm progression; oncogenes; ovarian neoplasms; quantitative polymerase chain reaction; staining; tissues
- Abstract:
- ... Laminin γ2 (LAMC2) is over-expressed in ovarian cancer, and its high expression facilitates cell invasion. Nevertheless, the effects of LAMC2 on other ovarian cancer cell functions and its underlying mechanism remain largely unclear. Bioinformatics analysis shows that LAMC2 is a predicted target of miR-125a-5p and miR-193a-3p. Therefore, the present study aimed to investigate the effects of LAMC2 ...
- DOI:
- 10.1016/j.lfs.2019.116648
- https://doi.org/10.1016/j.lfs.2019.116648