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bacterial antigens
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- Author:
- A. G. Korolyova-Ushakova; E. V. Baranova; S. G. Ignatov; P. V. Soloviev; N. N. Kondakov; T. M. Mel’nikova; P. I. Abronina; N. M. Podval’nyi; L. O. Kononov; S. F. Biketov
- Source:
- Applied biochemistry and microbiology 2019 v.55 no.6 pp. 696-703
- ISSN:
- 0003-6838
- Subject:
- Mycobacterium tuberculosis; bacterial antigens; blood serum; bovine serum albumin; epitopes; glycolipids; serodiagnosis; sugars; tuberculosis
- Abstract:
- ... Seven artificial mycobacterial antigens have been synthesized via the conjugation of synthetic carbohydrate epitopes of M. leprae phenolic glycolipid (PGL-1) and M. tuberculosis lipoarabinomannan (LAM) with bovine serum albumin (BSA). Each antigen has a different number of carbohydrate epitopes with a different structure and input sequence of the sugar residues. According to the serum reactivity, ...
- DOI:
- 10.1134/S0003683819060097
- https://doi.org/10.1134/S0003683819060097
- Author:
- Gowrisankar Rajam; George Carlone; Ellie Kim; Jin Choi; Simon Paulos; SoHee Park; Amilia Jeyachandran; Yamini Gorantla; Emily Wong; Amit Sabnis; Peter Browning; Rita Desai; Conrad P. Quinn; Jarad Schiffer
- Source:
- Biologicals 2019 v.57 pp. 9-20
- ISSN:
- 1045-1056
- Subject:
- Bordetella pertussis; adenylate cyclase; antibody specificity; bacterial antibodies; bacterial antigens; blood serum; enzyme-linked immunosorbent assay; hemagglutinins; microparticles; pertussis toxin; public health; quantitative analysis; reference standards; regression analysis; vaccination; vaccines
- Abstract:
- ... Despite wide spread vaccination, the public health burden of pertussis remains substantial. Current acellular pertussis vaccines comprise upto five Bordetella pertussis (Bp) antigens. Performing an ELISA to quantify antibody for each antigen is laborious and challenging to apply to pediatric samples where serum volume may be limited. We developed a microsphere based multiplex antibody capture assa ...
- DOI:
- 10.1016/j.biologicals.2018.11.001
- https://doi.org/10.1016/j.biologicals.2018.11.001
- Author:
- Muktiningsih Nurjayadi; Fera Kurnia Dewi; Irma Ratna Kartika; Umar Hasan; Ida Setianingsih; Nur asiah; Delia Ayu Wiguna; Anis Marcella; Fernita Puspasari; Asri Sulfianti; Kurnia Agustini; Wibowo Mangun Wardoyo; Hesham Ali El-Enshasy
- Source:
- Biocatalysis and agricultural biotechnology 2019 v.19 pp. 101157
- ISSN:
- 1878-8181
- Subject:
- antibodies; bacteria; bacterial antigens; blood; blood sampling; death; developing countries; drugs; immunoblotting; models; prototypes; recombinant proteins; typhoid fever
- Abstract:
- ... Typhoid fever is a world health problem, with 200,000 recorded death annually in developing countries. The discovery of new drug discovery and detection methods for typhoid is still continuing. In previous research, the 31 kilo Dalton (kDa) recombinant protein Fim-C-S typhi was successfully expressed. It was also reported that the recombinant protein Fim-C-S. typhi could induce the occurrence of a ...
- DOI:
- 10.1016/j.bcab.2019.101157
- https://doi.org/10.1016/j.bcab.2019.101157
- Author:
- Aadish Balouria; Sidhartha Deshmukh; H. S. Banga; Ajaz Ahmad; R. S. Brar; Sandeep Sodhi
- Source:
- Avian pathology 2019 v.48 no.2 pp. 168-177
- ISSN:
- 1465-3338
- Subject:
- Avibacterium paragallinarum; Coturnix japonica; antiserum; bacterial antigens; chickens; farmers; immunoelectrophoresis; immunohistochemistry; microorganisms; nasal cavity; pathogens; polyclonal antibodies; respiratory mucosa; swollen head syndrome; vaccines; virulence
- Abstract:
- ... Infectious coryza (IC) is often a curse for poultry farmers when it occurs concurrently with several pathogens causing swollen head syndrome. The disease is caused by Avibacterium paragallinarum, which inflicts initial damage to the nasal and respiratory epithelium. This facilitates the progression of disease pathology across the nasal cavity, thereby providing a platform for multiplication of opp ...
- DOI:
- 10.1080/03079457.2018.1562153
- https://doi.org/10.1080/03079457.2018.1562153
- Author:
- Ali Ehsani; Seyedeh S Naghibi; Majid Aminzare; Kobra Keykhosravi; Mohammad Hashemi
- Source:
- Journal of the science of food and agriculture 2019 v.99 no.5 pp. 2356-2364
- ISSN:
- 0022-5142
- Subject:
- Oncorhynchus mykiss; Pseudomonas fluorescens; Shewanella putrefaciens; antibodies; bacterial antigens; breast muscle; chicken meat; chitosan; coatings; cold storage; egg yolk; eggs; fish; fish fillets; immunoglobulin Y; microbial growth; natural additives; plate count; psychrophilic bacteria; raw fish; refrigeration; sensory properties; shelf life; storage time
- Abstract:
- ... BACKGROUND: This study investigated the effect of chitosan coating enriched with extracted egg yolk antibodies on microbial and sensory quality of rainbow trout fillet during refrigeration (4 ± 1 °C). Firstly, bacterial antigen suspensions (total psychrophilic bacteria, Pseudomonas fluorescens and Shewanella putrefaciens) were injected into the breast muscles of chickens. Eggs of immunized chicken ...
- DOI:
- 10.1002/jsfa.9442
- https://doi.org/10.1002/jsfa.9442
- Author:
- Saurabh Dubey; Biswajit Maiti; Sung‐Hyun Kim; Sangeetha Madambithara Sivadasan; Dhamotharan Kannimuthu; Pramod Kumar Pandey; Shivani Kallappa Girisha; Stephen Mutoloki; Shih‐Chu Chen; Øystein Evensen; Indrani Karunasagar; Hetron Mweemba Munang´andu
- Source:
- Journal of fish diseases 2019 v.42 no.6 pp. 835-850
- ISSN:
- 0140-7775
- Subject:
- Edwardsiella hoshinae; Edwardsiella ictaluri; Edwardsiella piscicida; Edwardsiella tarda; bacterial antigens; fish; fish diseases; genotyping; monophyly; phenotype; ribosomal RNA; vaccine development; vaccines; India; South Korea; Taiwan
- Abstract:
- ... The genus Edwardsiella is one of the major causes of fish diseases globally. Herein, we examined 37 isolates from ten different fish species from India, South Korea and Taiwan to gain insight into their phenotypic and genotypic properties, of which 30 were characterized as E. tarda with phenotypic homology estimated at 85.71% based on API‐20E biochemical tests. Genotyping using 16S rRNA put all is ...
- DOI:
- 10.1111/jfd.12984
- https://doi.org/10.1111/jfd.12984
- Author:
- Amy Flaxman; Yuko Yamaguchi; Pauline M. van Diemen; Christine Rollier; Elizabeth Allen; Elizaveta Elshina; David H. Wyllie
- Source:
- Vaccine 2019 v.37 no.3 pp. 494-501
- ISSN:
- 0264-410X
- Subject:
- Adenoviridae; Staphylococcus aureus; antibodies; antibody formation; bacterial antigens; blood serum; breeding; childhood; gastrointestinal system; immunoglobulin G; mice; microbial load; microbiome; microorganisms; monitoring; pathogens; vaccination; vaccines
- Abstract:
- ... S. aureus is a pathogen to which individuals are exposed shortly after birth, with immune responses to S. aureus increasing during childhood. There is marked heterogeneity between the anti- S. aureus immune responses of different humans, the basis of which is not fully understood.To investigate development of anti-S. aureus immune responses, we studied S. aureus colonised mice under controlled con ...
- DOI:
- 10.1016/j.vaccine.2018.11.063
- https://doi.org/10.1016/j.vaccine.2018.11.063
- Author:
- Hongsen Xu; Jing Xing; Xiaoqian Tang; Xiuzhen Sheng; Wenbin Zhan
- Source:
- Fish & shellfish immunology 2019 v.86 pp. 618-626
- ISSN:
- 1050-4648
- Subject:
- Paralichthys olivaceus; Vibrio anguillarum; antibodies; bacterial antigens; cell-mediated immunity; flounder; genes; immunoglobulin M; intramuscular injection; lymphocytes; models; muscles; outer membrane proteins; protective effect; recombinant vaccines; subunit vaccines; transcription (genetics); translation (genetics); vaccination
- Abstract:
- ... Outer membrane protein K (OmpK) is an immunogenic protein that could act as subunit vaccine candidate for Vibrio anguillarum. In this study, a DNA vaccine encoding the OmpK gene of V. anguillarum was constructed and confirmed to express OmpK in vitro and in vivo. To evaluate the potential of pcDNA3.1-OmpK (pOmpK) as vaccine candidate, the humoral and cellular immune responses, and protective effec ...
- DOI:
- 10.1016/j.fsi.2018.11.073
- https://doi.org/10.1016/j.fsi.2018.11.073
9. Investigating intestinal permeability and gut microbiota roles in acute coronary syndrome patients
- Author:
- Tarik Alhmoud; Anand Kumar; Chien-Chi Lo; Rana Al-Sadi; Stacey Clegg; Ihab Alomari; Tarek Zmeili; Cheryl Diane Gleasne; Kim Mcmurry; Armand Earl Ko Dichosa; Momchilo Vuyisich; Patrick Sam Guy Chain; Shiraz Mishra; Thomas Ma
- Source:
- Human microbiome journal 2019 v.13 pp. 100059
- ISSN:
- 2452-2317
- Subject:
- absorption barrier; bacterial antigens; biosynthesis; blood serum; case-control studies; dysbiosis; excretion; gamma-Proteobacteria; humans; intestinal microorganisms; intestines; lipopolysaccharides; metabolic syndrome; metabolites; microbiome; morbidity; mortality; obesity; patients; permeability; risk factors
- Abstract:
- ... Acute Coronary Syndrome (ACS) is a leading cause of morbidity and mortality. Perturbed gut-microbiota (dysbiosis) and increased intestinal permeability (leaky-gut) with translocation of bacterial antigens, play critical role in obesity and metabolic syndrome, which are also major ACS risk factors. Additionally, Trimethylamine-N-Oxide (TMAO), a metabolite produced by phylum Proteobacteria in gut is ...
- DOI:
- 10.1016/j.humic.2019.100059
- https://doi.org/10.1016/j.humic.2019.100059
- Author:
- Ana Vitlic; Sohaib Sadiq; Hafiz I. Ahmed; Elisa C. Ale; Ana G. Binetti; Andrew Collett; Paul N. Humpreys; Andrew P. Laws
- Source:
- Carbohydrate research 2019 v.476 pp. 44-52
- ISSN:
- 0008-6215
- Subject:
- Crohn disease; Lactobacillus fermentum; Toll-like receptor 2; aqueous solutions; bacterial antigens; beta-glucans; cell proliferation; colitis; exopolysaccharides; gel chromatography; molecular weight; mononuclear leukocytes; nuclear magnetic resonance spectroscopy; tumor necrosis factor-alpha
- Abstract:
- ... When grown in a semi-defined medium, L. fermentum Lf2 synthesizes significant quantities (∼2 g/L) of two exopolysaccharides (EPS). The two EPS were separated by preparative size exclusion chromatography to give a high molecular mass β-glucan (1.23 × 106 Da) and a medium molecular mass heteroglycan (8.8 × 104 Da). The structure of the high molecular mass β-glucan was determined using a combination ...
- DOI:
- 10.1016/j.carres.2019.03.003
- https://doi.org/10.1016/j.carres.2019.03.003
- Author:
- Mengjuan Wu; Zhui Tu; Fengchun Huang; Qinghua He; Jinheng Fu; Yanping Li
- Source:
- Biochemical and biophysical research communications 2019 v.512 no.3 pp. 531-536
- ISSN:
- 0006-291X
- Subject:
- DNA libraries; Vibrio fluvialis; animal pathogens; bacteria; bacterial antigens; bacteriophages; developing countries; diarrhea; enteritis; enzyme-linked immunosorbent assay; humans; lipopolysaccharides; plate count; rapid methods; seawater; serotypes
- Abstract:
- ... Vibrio fluvialis is considered as a human pathogen in developing countries. This bacterium is widely distributed in seawater and harbors that contains traces of salt. V. fluvialis can cause human enteritis and diarrhea, which has broken out at a global scale. Lipopolysaccharide (LPS) is a key bacterial antigen used to classify V. fluvialis serogroups. In this research, phage display technology was ...
- DOI:
- 10.1016/j.bbrc.2019.03.104
- https://doi.org/10.1016/j.bbrc.2019.03.104
12. Partial protection induced by Salmonella based Brucella vaccine candidate in pregnant guinea pigs
- Author:
- Jonathan Lalsiamthara; Amal Senevirathne; John Hwa Lee
- Source:
- Vaccine 2019 v.37 no.7 pp. 899-902
- ISSN:
- 0264-410X
- Subject:
- Brucella; Salmonella; abortion (animals); animal models; bacterial antigens; guinea pigs; histopathology; humoral immunity; immunoglobulin G; induced abortion; live vaccines; liver; pregnancy; sows; spleen; vaccination; virulence
- Abstract:
- ... Residual virulence is a major drawback in current Brucella vaccines. Live vaccines induce abortions in pregnant animals. Hence, a novel anti-Brucella vaccine was developed utilizing rough Salmonella delivering four Brucella antigens. Safety implications during pregnancy, humoral immune responses, and protective efficacy against wild type Brucella was investigated in guinea pig model. The vaccine d ...
- DOI:
- 10.1016/j.vaccine.2019.01.020
- https://doi.org/10.1016/j.vaccine.2019.01.020
- Author:
- Tjerko Kamminga; Simen-Jan Slagman; Vitor A.P. Martins dos Santos; Jetta J.E. Bijlsma; Peter J. Schaap
- Source:
- Trends in biotechnology 2019 pp. -
- ISSN:
- 0167-7799
- Subject:
- bacterial antigens; bacterial vaccines; biochemical pathways; bioengineering; models; process design; screening
- Abstract:
- ... Design of a reliable process for bacterial antigen production requires understanding of and control over critical process parameters. Current methods for process design use extensive screening experiments for determining ranges of critical process parameters yet fail to give clear insights into how they influence antigen potency. To address this gap, we propose to apply constraint-based genome-sca ...
- DOI:
- 10.1016/j.tibtech.2019.03.005
- https://doi.org/10.1016/j.tibtech.2019.03.005
- Author:
- Moonika H. Marana; Dagoberto Sepúlveda; Defang Chen; Azmi Al-Jubury; Rzgar M. Jaafar; Per W. Kania; Niels Henrik Henriksen; Bjørn Krossøy; Inger Dalsgaard; Niels Lorenzen; Kurt Buchmann
- Source:
- Fish & shellfish immunology 2019 v.88 pp. 344-351
- ISSN:
- 1050-4648
- Subject:
- Aeromonas salmonicida subsp. salmonicida; Oncorhynchus mykiss; Vibrio anguillarum; Yersinia ruckeri; antibiotics; antibody formation; bacterial antigens; disease outbreaks; fish cages; fish culture; freshwater; furunculosis; gene expression; interleukin-1beta; interleukin-6; interleukin-8; mariculture; pathogens; trout; vaccination; vaccines; vibriosis; yersiniosis; Denmark
- Abstract:
- ... Mariculture in Denmark is based on production of rainbow trout grown two years in fresh water followed by one growth season in sea cages. Although the majority of rainbow trout are vaccinated against the most serious bacterial pathogens – Aeromonas salmonicida subsp. salmonicida, Vibrio anguillarum and Yersinia ruckeri, by the use of commercially available vaccines, disease outbreaks requiring tre ...
- DOI:
- 10.1016/j.fsi.2019.03.001
- https://doi.org/10.1016/j.fsi.2019.03.001
- Author:
- Rod J. Dillon
- Source:
- Comptes rendus 2019 v.342 no.7-8 pp. 272-273
- ISSN:
- 1631-0691
- Subject:
- Acetobacteraceae; Culicidae; Leishmania mexicana; Lutzomyia longipalpis; aircraft; airports; antileishmanials; bacteria; bacterial antigens; bacterial colonization; bed nets; blood; boys; children; digestive system; drug development; eggs; entomologists; entomopathogens; experts; females; funding; hosts; humans; imagos; immune system; inflammasomes; insect control; insect vectors; intestinal microorganisms; issues and policy; languages; larvae; leishmaniasis; males; microbial ecology; models; mosquito nets; niches; parasites; plants (botany); pupae; social networks; travel; tropical diseases; yeasts; United Kingdom
- Abstract:
- ... It has been hundred years since Phlebotomine sand flies were first identified as transmitters of the medically important parasites called Leishmania. The key players, investigated by scientists during the first 60 years, were the insect, the parasite, and the mammalian host. Forty years ago, plants were included as potentially influential players in the transmission process. During the past ten ye ...
- DOI:
- 10.1016/j.crvi.2019.09.025
- https://doi.org/10.1016/j.crvi.2019.09.025