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- Author:
- Mu Luo; Lin Zhou; Shi-jie Zhan; Li-juan Cheng; Ruo-nan Li; Hui wang; Bin Liu; Hua Linghu
- Source:
- Biochemical and biophysical research communications 2019 v.513 no.2 pp. 528-533
- ISSN:
- 0006-291X
- Subject:
- animal ovaries; cell lines; cell movement; gene ontology; gene overexpression; genes; genetic disorders; histology; ligands; neoplasm cells; ovarian neoplasms; patients; small interfering RNA
- Abstract:
- ... The ALPL gene is linked to hypophosphatasia, a rare genetic disease. Owing to the inverse relationships between ALPL expression and both the International Federation of Gynecology and Obstetrics (FIGO) stages and histological grades assigned to patients with serous ovarian cancer (SOC), this study was designed to explore the role and possible mechanisms of ALPL in cell motility of high grade SOC ( ...
- DOI:
- 10.1016/j.bbrc.2019.04.016
- https://doi.org/10.1016/j.bbrc.2019.04.016
- Author:
- Kohei Kaneyoshi; Keiji Uchiyama; Masayoshi Onitsuka; Noriko Yamano; Yuichi Koga; Takeshi Omasa
- Source:
- Journal of bioscience and bioengineering 2019 v.127 no.1 pp. 107-113
- ISSN:
- 1389-1723
- Subject:
- Chinese hamsters; animal ovaries; antibodies; antibody formation; biopharmaceuticals; cell culture; cell lines; endoplasmic reticulum; engineering; gel chromatography; immunoglobulin G; microscopy; secretion
- Abstract:
- ... The production of biopharmaceutical immunoglobulin G (IgG) using cultured mammalian cells, especially Chinese hamster ovary (CHO) cells is well established and has been markedly improved through the modification of cells and cell culture engineering technologies. The establishment of high-production cell lines remains a challenge. The intracellular secretion of IgG has been investigated to identif ...
- DOI:
- 10.1016/j.jbiosc.2018.06.018
- https://doi.org/10.1016/j.jbiosc.2018.06.018
- Author:
- Cyrielle Calmels; Solène Arnoult; Bassem Ben Yahia; Laetitia Malphettes; Mikael Rørdam Andersen
- Source:
- Metabolic Engineering Communications 2019 pp. -
- ISSN:
- 2214-0301
- Subject:
- Chinese hamsters; animal ovaries; biopharmaceuticals; cell lines; engineering; glycolysis; lipid metabolism; models; monoclonal antibodies; oxidative phosphorylation; pentose phosphate cycle; phenotype; tricarboxylic acid cycle
- Abstract:
- ... Biopharmaceutical industrial processes are based on high yielding stable recombinant Chinese Hamster Ovary (CHO) cells that express monoclonal antibodies. However, the process and feeding regimes need to be adapted for each new cell line, as they all have a slightly different metabolism and product performance. A main limitation for accelerating process development is that the metabolic pathways u ...
- DOI:
- 10.1016/j.mec.2019.e00097
- PubMed:
- 31720213
- PubMed Central:
- PMC6838488
- https://doi.org/10.1016/j.mec.2019.e00097
- Author:
- Christian Seca; Alessandra Ferraresi; Suratchanee Phadngam; Chiara Vidoni; Ciro Isidoro
- Source:
- Journal of materials chemistry B 2019 v.7 no.35 pp. 5376-5391
- ISSN:
- 2050-7518
- Subject:
- adverse effects; animal ovaries; autophagy; cell lines; chemistry; fluorescence; genes; genetic background; mitochondria; nanoparticles; neoplasm cells; ovarian neoplasms; polystyrenes; proteins; risk; stress response; therapeutics; toxicity
- Abstract:
- ... The use of nanoparticles (NPs) for diagnostic and therapeutic purposes involves the risk of side effects due to the presence of reactive groups on their surface. We studied the cellular stress response to spheroid fluorescent polystyrene nanoparticles (PS-NPs) functionalized with Amino groups in two ovarian cancer cell lines differing in the expression, among others, of relevant proteins involved ...
- DOI:
- 10.1039/c9tb00935c
- https://doi.org/10.1039/c9tb00935c
- Author:
- Sara Pereira; Daniel Ley; Mikkel Schubert; Lise Marie Grav; Helene Faustrup Kildegaard; Mikael Rørdam Andersen
- Source:
- Journal of biotechnology 2019 v.306 pp. 24-31
- ISSN:
- 0168-1656
- Subject:
- Chinese hamsters; animal ovaries; branched chain amino acids; byproducts; cell growth; cell lines; essential amino acids; genes; isoleucine; leucine; metabolites; product quality; protein synthesis; recombinant proteins; toxic substances; transferases; valine
- Abstract:
- ... In recombinant protein expression using Chinese hamster ovary (CHO) cells, chemically defined media contain essential amino acids such as branched chain amino acids (BCAAs) leucine, isoleucine and valine. Availability of amino acids is critical as these are building blocks for protein synthesis. However, breakdown of amino acids can lead to build up of toxic intermediates and metabolites that decr ...
- DOI:
- 10.1016/j.jbiotec.2019.08.017
- https://doi.org/10.1016/j.jbiotec.2019.08.017
- Author:
- Haiqin SongThe two authors contributed equally to this work.; Feifei Quan; Zhiqiang Yu; Minhua Zheng; Yan Ma; Haihua Xiao; Fang Ding
- Source:
- Journal of materials chemistry B 2019 v.7 no.3 pp. 433-442
- ISSN:
- 2050-7518
- Subject:
- adverse effects; animal ovaries; antineoplastic activity; cell lines; chemistry; cisplatin; cytotoxicity; drug resistance; endocytosis; in vivo studies; iron oxides; magnetic fields; nanocarriers; nanoparticles; neoplasm cells; ovarian neoplasms; platinum; toxicity testing; zeta potential
- Abstract:
- ... Platinum (Pt)-based drugs including cisplatin, carboplatin and oxaliplatin have been widely used as first-line anticancer regimens due to their excellent anticancer efficacy. However, the clinical application of these drugs is greatly limited due to their side effects and drug resistance. In this study, an antitumor drug delivery system based on carboplatin prodrug loading Fe₃O₄ nanoparticles (NPs ...
- DOI:
- 10.1039/c8tb02574f
- https://doi.org/10.1039/c8tb02574f
- Author:
- Danzi Song; Jiwei Cui; Yi Ju; Matthew Faria; Huanli Sun; Christopher B. Howard; Kristofer J. Thurecht; Frank Caruso
- Source:
- ACS applied materials & interfaces 2019 v.11 no.32 pp. 28720-28731
- ISSN:
- 1944-8252
- Subject:
- Chinese hamsters; animal ovaries; antibodies; breast neoplasms; cell lines; dosimetry; epidermal growth factor receptors; humans; macrophages; mice; mixing; neoplasm cells; polyethylene glycol; surface area
- Abstract:
- ... In the present study, a capsule system that consists of a stealth carrier based on poly(ethylene glycol) (PEG) and functionalized with bispecific antibodies (BsAbs) is introduced to examine the influence of the capsule shape and size on cellular targeting. Hollow spherical and rod-shaped PEG capsules with tunable aspect ratios (ARs) of 1, 7, and 18 were synthesized and subsequently functionalized ...
- DOI:
- 10.1021/acsami.9b10304
- https://doi.org/10.1021/acsami.9b10304
- Author:
- Rossina Domínguez-Ríos; Dante R. Sánchez-Ramírez; Kassandra Ruiz-Saray; Paola E. Oceguera-Basurto; Mario Almada; Josué Juárez; Abraham Zepeda-Moreno; Alicia del Toro-Arreola; Antonio Topete; Adrián Daneri-Navarro
- Source:
- Colloids and surfaces 2019 v.178 pp. 199-207
- ISSN:
- 0927-7765
- Subject:
- animal ovaries; breast neoplasms; cell lines; cisplatin; clinical trials; cytotoxicity; drug therapy; epithelium; fluorescein; fluorescence; genetic markers; monoclonal antibodies; multiple drug resistance; nanoparticles; neoplasm cells; ovarian neoplasms; patients; receptors; resistance mechanisms; taxanes; zeta potential
- Abstract:
- ... The conventional treatment (cytoreduction combined with cisplatin/carboplatin and taxane drugs) of ovarian cancer has a high rate of failure and recurrence despite a favorable initial response. This lack of success is usually attributed to the development of multidrug resistance mechanisms by cancer cells and avoidance of the anti-growth effects of monoclonal targeted therapeutic antibodies. The d ...
- DOI:
- 10.1016/j.colsurfb.2019.03.011
- https://doi.org/10.1016/j.colsurfb.2019.03.011
9. Comparing two automated high throughput viable-cell counting systems for cell culture applications
- Author:
- Séverine Fagète; Cédric Steimer; Pierre-Alain Girod
- Source:
- Journal of biotechnology 2019 v.305 pp. 23-26
- ISSN:
- 0168-1656
- Subject:
- Chinese hamsters; animal ovaries; automation; biopharmaceuticals; business enterprises; cell culture; cell lines; cell viability; counters; labor; monitoring
- Abstract:
- ... Cell counting and viability assessment is an integral part of mammalian cell line development. While manual counting with a hemocytometer is still the gold standard method, its subjectivity and high labor intensity has resulted in its reduced use in favor of automated systems. In addition, some of these automated systems offer multiwell plate based high throughput cell count, which is an asset for ...
- DOI:
- 10.1016/j.jbiotec.2019.08.014
- https://doi.org/10.1016/j.jbiotec.2019.08.014
- Author:
- Hamideh Parhiz; Stephanie A. Ketcham; Guozhang Zou; Bidesh Ghosh; Erica J. Fratz-Berilla; Muhammad Ashraf; Tongzhong Ju; Chikkathur N. Madhavarao
- Source:
- Applied microbiology and biotechnology 2019 v.103 no.15 pp. 6081-6095
- ISSN:
- 0175-7598
- Subject:
- Chinese hamsters; animal ovaries; beta-glucuronidase; bioreactors; butyrates; capillary electrophoresis; cell lines; drugs; enzyme replacement therapy; matrix-assisted laser desorption-ionization mass spectrometry; molecular models; pH; protein synthesis; purification methods; temperature
- Abstract:
- ... β-Glucuronidase is a lysosomal enzyme and a molecular model of a class of therapeutics approved as enzyme replacement therapies for lysosomal storage diseases. Understanding the effect of bioreactor process variables on the production and quality of the biologics is critical for maintaining quality and efficacy of the biotherapeutics. Here, we have investigated the effect of three process variable ...
- DOI:
- 10.1007/s00253-019-09889-7
- https://doi.org/10.1007/s00253-019-09889-7
- Author:
- Najmeh Jooyan; Bahram Goliaei; Bahareh Bigdeli; Reza Faraji-Dana; Ali Zamani; Milad Entezami; Seyed Mohammad Javad Mortazavi
- Source:
- Environmental research 2019 v.174 pp. 176-187
- ISSN:
- 0013-9351
- Subject:
- DNA damage; absorption; adverse effects; animal ovaries; cell death; cell lines; culture media; direct contact; electromagnetic field; energy; hamsters; ionizing radiation; membrane permeability; mitosis; mobile telephones; olives; radio waves; reactive oxygen species
- Abstract:
- ... The rapid rise in global concerns about the adverse health effects of exposure to radiofrequency radiation (RFR) generated by common devices such as mobile phones has prompted scientists to further investigate the biological effects of these environmental exposures. Non-targeted effects (NTEs) are responses which do not need a direct exposure to be expressed and are particularly significant at low ...
- DOI:
- 10.1016/j.envres.2019.03.063
- https://doi.org/10.1016/j.envres.2019.03.063
- Author:
- Bhuvana S. Doddapaneni; Adel M. AL-Fatease; Deepa A. Rao; Adam W.G. Alani
- Source:
- Journal of controlled release 2019 v.307 pp. 272-281
- ISSN:
- 0168-3659
- Subject:
- anaerobic conditions; angiogenesis; animal models; animal ovaries; apoptosis; cell lines; composite polymers; drug resistance; drugs; evaporation; hypoxia; immunohistochemistry; in vitro studies; mammals; micelles; mutation; ovarian neoplasms; phenotype; polyethylene glycol; rapamycin; remission; signal transduction; solubility; solvents; therapeutics
- Abstract:
- ... Mutations in the tumor protein (TP53) and the mammalian target of rapamycin (mTOR) pathway have been elucidated as driver mutations in ovarian carcinomas that transform into an invasive phenotype under hypoxic conditions. Chetomin (CHE) targets the hypoxic pathway while Everolimus (EVR) acts on the mTOR pathway. Poor aqueous solubilities of both compounds limit their clinical applications. Diblock ...
- DOI:
- 10.1016/j.jconrel.2019.06.036
- https://doi.org/10.1016/j.jconrel.2019.06.036
- Author:
- Jidapa Yamkasem; Puntanat Tattiyapong; Attapon Kamlangdee; Win Surachetpong
- Source:
- Journal of fish diseases 2019 v.42 no.9 pp. 1293-1300
- ISSN:
- 0140-7775
- Subject:
- RNA; Tilapia lake virus; animal ovaries; biosecurity; breeding stock; cell lines; fish diseases; fish fry; genomics; hatcheries; in situ hybridization; liver; progeny; testes; virus transmission; viruses
- Abstract:
- ... Tilapia lake virus disease (TiLVD) is an emerging viral disease in tilapia with worldwide distribution. Although the horizontal transmission of TiLV has been demonstrated through the cohabitation of infected fish with susceptible fish, no direct experiment showed the potential of vertical transmission from broodstock to progeny. In this study, natural outbreaks of TiLV in broodstock and fry in two ...
- DOI:
- 10.1111/jfd.13050
- https://doi.org/10.1111/jfd.13050
- Author:
- Kasra X. Ramyar; Xin Xu; Natalie M. White; Andrew Keightley; Brian V. Geisbrecht
- Source:
- Journal of immunological methods 2019 pp. -
- ISSN:
- 0022-1759
- Subject:
- Chinese hamsters; Tobacco etch virus; animal ovaries; cell lines; complement; culture media; gel chromatography; homeostasis; humans; innate immunity; ligands; proteinases; proteolysis; surface plasmon resonance; surface proteins
- Abstract:
- ... The complement system consists of a series of soluble and cell-surface proteins that serve numerous roles in innate immunity, development, and homeostasis. Despite its many functions, the central event in the complement system is the proteolytic activation of the 185 kDa complement component 3 (C3) into its opsonin and anaphylatoxin fragments known as C3b (175 kDa) and C3a (10 kDa), respectively. ...
- DOI:
- 10.1016/j.jim.2019.07.005
- PubMed:
- 31319063
- PubMed Central:
- PMC6707835
- https://doi.org/10.1016/j.jim.2019.07.005
- Author:
- Linda Schwaigerlehner; Patrick Mayrhofer; Matthias Diem; Willibald Steinfellner; Emma Fenech; Chris Oostenbrink; Renate Kunert
- Source:
- Applied microbiology and biotechnology 2019 v.103 no.18 pp. 7505-7518
- ISSN:
- 0175-7598
- Subject:
- Chinese hamsters; amino acid sequences; animal ovaries; cell lines; germ cells; hydrophobicity; loci; models; monoclonal antibodies; protein engineering; somatic mutation; thermal stability
- Abstract:
- ... The production potential of recombinant monoclonal antibody (mAb) expressing cell lines depends, among other factors, on the intrinsic antibody structure determined by the amino acid sequence. In this study, we investigated the influence of somatic mutations in the V(D)J sequence of four individual, mature model mAbs on the expression potential. Therefore, we defined four couples, each consisting ...
- DOI:
- 10.1007/s00253-019-09998-3
- PubMed:
- 31350616
- PubMed Central:
- PMC6719414
- https://doi.org/10.1007/s00253-019-09998-3
- Author:
- Ga-Bin Park; Jee-Yeong Jeong; Daejin Kim
- Source:
- Marine drugs 2019 v.17 no.7 pp. -
- ISSN:
- 1660-3397
- Subject:
- animal ovaries; apoptosis; autophagy; cell lines; death; drug resistance; gene silencing; gliotoxins; marine fungi; membrane potential; mitochondrial membrane; neoplasm cells; ovarian neoplasms; paclitaxel; protein kinases; proteins; signal transduction; transcription (genetics)
- Abstract:
- ... Death-associated protein kinase 1 (DAPK1) expression induced by diverse death stimuli mediates apoptotic activity in various cancers, including ovarian cancer. In addition, mutual interaction between the tumor suppressor p53 and DAPK1 influences survival and death in several cancer cell lines. However, the exact role and connection of DAPK1 and p53 family proteins (p53, p63, and p73) in drug-resis ...
- DOI:
- 10.3390/md17070412
- PubMed:
- 31336860
- PubMed Central:
- PMC6669733
- https://doi.org/10.3390/md17070412
- Author:
- Aydan Dag; Pinar Sinem Omurtag Ozgen; Sezen Atasoy
- Source:
- Biomacromolecules 2019 v.20 no.8 pp. 2962-2972
- ISSN:
- 1526-4602
- Subject:
- Chinese hamsters; adverse effects; animal ovaries; antineoplastic agents; breast neoplasms; cell lines; composite polymers; cytotoxicity; drug therapy; glycosylation; hydrophobicity; kidney neoplasms; light scattering; moieties; nanocarriers; nanoparticles; neoplasm cells; platinum; polymerization; prostatic neoplasms; toxicity testing; transmission electron microscopy
- Abstract:
- ... An important requirement to decrease the side effects of chemotherapy drugs is to develop nanocarriers with precise biological functions. In this work, a set of glyconanoparticles was prepared via self-assembly of amphiphilic glycoblock copolymers for the targeted delivery of a hydrophobic chemotherapy drug. Well-defined glycoblock copolymers that consist of 1,1-di-tert-butyl 3-(2-(metyloyloxy)eth ...
- DOI:
- 10.1021/acs.biomac.9b00528
- https://doi.org/10.1021/acs.biomac.9b00528
- Author:
- Narumol Khomkhum; Somphob Leetachewa; Aulia Rahmi Pawestri; Saengduen Moonsom
- Source:
- Parasites & vectors 2019 v.12 no.1 pp. 101
- ISSN:
- 1756-3305
- Subject:
- Chinese hamsters; Entamoeba histolytica; adhesion; animal models; animal ovaries; antibodies; cell adhesion; cell lines; colon; cytoskeletal proteins; dysentery; epithelium; humans; immune response; immune system; immunization; immunoglobulin G; liver abscess; membrane proteins; mice; parasites; plasma membrane; protein composition; surface proteins; trophozoites; virulence
- Abstract:
- ... BACKGROUND: Despite similarities in morphology, gene and protein profiles, Entamoeba histolytica and E. moshkovskii show profound differences in pathogenicity. Entamoeba histolytica infection might result in amoebic dysentery and liver abscess, while E. moshkovskii causes only mild diarrhea. Extensive studies focus on roles of host immune responses to the pathogenic E. histolytica; however, eviden ...
- DOI:
- 10.1186/s13071-019-3363-5
- PubMed:
- 30867040
- PubMed Central:
- PMC6416977
- https://doi.org/10.1186/s13071-019-3363-5
- Author:
- Kaiming Chen; Dong Li; Hongwen Li; Bing Li; Ruiqin Wang; Lingxiao Jiang; Lei Huang; Xiaoqing Xu; Jie Li; Fei Teng; Cizhong Jiang; Hua Gu; Jianmin Fang
- Source:
- Biotechnology letters 2019 v.41 no.6-7 pp. 719-732
- ISSN:
- 0141-5492
- Subject:
- Chinese hamsters; RNA; animal ovaries; cell lines; gene expression regulation; genes; protein synthesis; protein transport; recombinant proteins; sequence analysis; transcription (genetics); transcriptome
- Abstract:
- ... OBJECTIVES: To identify genes that affected protein expression in Chinese hamster ovary (CHO) cells was significant, and we identified the changes in the transcriptome and the functional gene sets that would contribute to increase expression of recombinant protein. RESULTS: Here two sub-clones from a methotrexate-treated parental recombinant CHO cell line were selected. The two sub-clones, with di ...
- DOI:
- 10.1007/s10529-019-02681-7
- https://doi.org/10.1007/s10529-019-02681-7
- Author:
- Che Lin Kim; Gyun Min Lee
- Source:
- Metabolic engineering 2019 v.52 pp. 57-67
- ISSN:
- 1096-7176
- Subject:
- Chinese hamsters; animal ovaries; autocrine signaling; bone morphogenetic proteins; cell lines; clones; gene editing; gene expression; genes; growth retardation; humans; messenger RNA; methotrexate; signal transduction
- Abstract:
- ... A Chinese hamster ovary (CHO) cell line producing recombinant human bone morphogenetic protein-4 (rhBMP-4) (CHO-BMP-4), which expresses essential components of BMP signal transduction, underwent autocrine BMP-4 signaling. RNA seq analysis on CHO host cells (DG44) treated with rhBMP-4 (20 µg/mL) suggested that rhBMP-4 induced signaling in CHO cells could be a critical factor in limiting rhBMP-4 pro ...
- DOI:
- 10.1016/j.ymben.2018.11.003
- https://doi.org/10.1016/j.ymben.2018.11.003
- Author:
- Katarina Michalcova; Shubhadeep Roychoudhury; Marek Halenar; Eva Tvrda; Eva Kovacikova; Jaromir Vasicek; Peter Chrenek; Simona Baldovska; Luboslav Sanislo; Vladimir Kren; Adriana Kolesarova
- Source:
- Journal of environmental science and health 2019 v.54 no.9 pp. 752-757
- ISSN:
- 1532-4109
- Subject:
- animal ovaries; apoptosis; cell lines; cell viability; chemiluminescence; dietary supplements; enzyme-linked immunosorbent assay; flow cytometry; isoquercitrin; neoplasm cells; ovarian neoplasms; oxidative stress; reactive oxygen species; transforming growth factor beta 1
- Abstract:
- ... Isoquercitrin is a dietary bioflavonoid used as a food supplement. We studied the mechanism underlying its effect in human ovarian cancer cells using OVCAR-3 cell line. Viability, survival, apoptosis, release of human transforming growth factor-β1 (TGF-β1) and TGF-β1 receptor, and intracellular reactive oxygen species (ROS) generation by OVCAR-3 cells were examined after isoquercitrin treatment at ...
- DOI:
- 10.1080/03601234.2019.1633214
- https://doi.org/10.1080/03601234.2019.1633214
- Author:
- Junqing He; Junyan Wang; Nan Zhang; Luyao Shen; Linlin Wang; Xiao Xiao; Yan Wang; Tao Bing; Xiangjun Liu; Songqing Li; Dihua Shangguan
- Source:
- Talanta 2019 v.194 pp. 437-445
- ISSN:
- 0039-9140
- Subject:
- animal ovaries; blood serum; cell lines; dissociation; drug resistance; glycoproteins; humans; ions; ligands; neoplasm cells; nucleases; nucleotide aptamers; ovarian neoplasms; prognosis; survival rate; temperature; therapeutics
- Abstract:
- ... Ovarian cancer is regarded as the most lethal gynecologic malignancy with poor prognosis and high mortality rate. Drug-resistance was thought to be the main obstacle to improving overall survival rate of ovarian cancer. New ligands for drug-resistant ovarian cancer cells have potential for the development of diagnosis and therapy of ovarian cancer. In present work, we reported two aptamers, HF3–58 ...
- DOI:
- 10.1016/j.talanta.2018.10.028
- https://doi.org/10.1016/j.talanta.2018.10.028
- Author:
- Orla Coleman; Srinivas Suda; Justine Meiller; Michael Henry; Markus Riedl; Niall Barron; Martin Clynes; Paula Meleady
- Source:
- Journal of proteomics 2019 v.195 pp. 23-32
- ISSN:
- 1874-3919
- Subject:
- animal ovaries; biogenesis; biopharmaceuticals; cell growth; cell lines; engineering; hamsters; immunoglobulin G; liquid chromatography; microRNA; phenotype; protein synthesis; proteome; proteomics; recombinant proteins; ribosomes; tandem mass spectrometry
- Abstract:
- ... Cell line engineering using microRNAs represents a desirable route for improving the efficiency of recombinant protein production by CHO cells. In this study we generated stable CHO DP12 cells expressing a miR-7 sponge transcript which sequesters miR-7 from its endogenous targets. Depletion of miR-7 results in a 65% increase in cell growth and >3-fold increase in yield of secreted IgG protein. Qua ...
- DOI:
- 10.1016/j.jprot.2019.01.003
- https://doi.org/10.1016/j.jprot.2019.01.003
- Author:
- Michael Kleemann; Helga Schneider; Kristian Unger; Jeremias Bereuther; Simon Fischer; Philip Sander; E. Marion Schneider; Pamela Fischer-Posovszky; Christian U. Riedel; René Handrick; Kerstin Otte
- Source:
- Cellular and molecular life sciences 2019 v.76 no.3 pp. 539-559
- ISSN:
- 1420-682X
- Subject:
- DNA; animal ovaries; apoptosis; caspases; cell lines; gene expression regulation; luciferase; membrane potential; messenger RNA; microRNA; mitochondrial membrane; neoplasm cells; ovarian neoplasms; phosphorylation; protein synthesis; protein-serine-threonine kinases; proto-oncogenes; serine; signal transduction; threonine; transcription (genetics); transfection
- Abstract:
- ... Apoptosis is a form of directed programmed cell death with a tightly regulated signalling cascade for the destruction of single cells. MicroRNAs (miRNAs) play an important role as fine tuners in the regulation of apoptotic processes. MiR-493-3p mimic transfection leads to the induction of apoptosis causing the breakdown of mitochondrial membrane potential and the activation of Caspases resulting i ...
- DOI:
- 10.1007/s00018-018-2958-x
- https://doi.org/10.1007/s00018-018-2958-x
- Author:
- Jinsha Koroth; Snehal Nirgude; Shweta Tiwari; Vidya Gopalakrishnan; Raghunandan Mahadeva; Sujeet Kumar; Subhas S. Karki; Bibha Choudhary
- Source:
- BMC complementary and alternative medicine 2019 v.19 no.1 pp. 273
- ISSN:
- 1472-6882
- Subject:
- Western blotting; adverse effects; alternative medicine; animal ovaries; antineoplastic activity; apoptosis; bioavailability; breast neoplasms; cell lines; cell movement; curcumin; cytotoxicity; flow cytometry; interstitial collagenase; liquids; metastasis; neoplasm cells; ovarian neoplasms; stem cells; toxicity testing
- Abstract:
- ... BACKGROUND: Curcumin is known for its multitude of medicinal properties, including anti-cancer and migrastatic activity. Efforts to overcome poor bioavailability, stability, and side effects associated with the higher dose of curcumin has led to the development of newer derivatives of curcumin. Thus, the focus of this study is to screen novel curcumin derivatives, namely ST03 and ST08, which have ...
- DOI:
- 10.1186/s12906-019-2685-3
- PubMed:
- 31638975
- PubMed Central:
- PMC6802352
- https://doi.org/10.1186/s12906-019-2685-3
- Author:
- Dongya Zhang; Hongjun Guo; Wei Feng; Haifeng Qiu
- Source:
- Life sciences 2019 v.232 pp. 116648
- ISSN:
- 0024-3205
- Subject:
- Western blotting; animal ovaries; apoptosis; bioinformatics; carcinogenesis; cell lines; cell proliferation; flow cytometry; gene expression regulation; gene overexpression; laminin; luciferase; mitogen-activated protein kinase; neoplasm cells; neoplasm progression; oncogenes; ovarian neoplasms; quantitative polymerase chain reaction; staining; tissues
- Abstract:
- ... Laminin γ2 (LAMC2) is over-expressed in ovarian cancer, and its high expression facilitates cell invasion. Nevertheless, the effects of LAMC2 on other ovarian cancer cell functions and its underlying mechanism remain largely unclear. Bioinformatics analysis shows that LAMC2 is a predicted target of miR-125a-5p and miR-193a-3p. Therefore, the present study aimed to investigate the effects of LAMC2 ...
- DOI:
- 10.1016/j.lfs.2019.116648
- https://doi.org/10.1016/j.lfs.2019.116648
- Author:
- Changzhi Xu; Qianqian Han; Qin Zhou; Lanlan Zhang; Pengfei Wu; Yafang Lu; Yan Si; Tengfei Ma; Bingbing Ma; Buchang Zhang
- Source:
- Applied microbiology and biotechnology 2019 v.103 no.17 pp. 7085-7095
- ISSN:
- 0175-7598
- Subject:
- Chinese hamsters; animal ovaries; antibodies; antibody formation; apoptosis; bioinformatics; cell cycle; cell lines; cell viability; engineering; enzyme inhibition; enzymes; gene overexpression; microRNA; therapeutics; transfection
- Abstract:
- ... MicroRNAs (miRNAs) function as important regulators of major cellular processes, such as cell cycle, proliferation, development, and apoptosis. Recently, miRNA engineering of Chinese hamster ovary (CHO) cells has emerged as a promising strategy for enhancing therapeutic antibody production. Previously, we have reported that inhibition of deubiquitinase cylindromatosis (CYLD) remarkably enhanced th ...
- DOI:
- 10.1007/s00253-019-10000-3
- https://doi.org/10.1007/s00253-019-10000-3
- Author:
- Yuan Wang; Xia Lei; Chengying Gao; Yanxia Xue; Xiaolin Li; Haiying Wang; Yan Feng
- Source:
- Journal of biosciences 2019 v.44 no.6 pp. 126
- ISSN:
- 0250-5991
- Subject:
- Western blotting; animal ovaries; apoptosis; cell growth; cell lines; cell proliferation; epithelial cells; fallopian tubes; flow cytometry; immunohistochemistry; interphase; luciferase; microRNA; neoplasm cells; ovarian neoplasms; protein content; tissues; tumor suppressor genes; women
- Abstract:
- ... MicroRNAs have been reported to play a crucial role in ovarian cancer (OC) as the most lethal malignancy of the women. Here, we found miR-506-3p was significantly down-regulated in OC tissues compared with corresponding adjacent non-tumor tissues. Ectopic miR-506-3p expression inhibited OC cell growth and proliferation using MTT and colony formation assay. Additionally, flow cytometry analysis sho ...
- DOI:
- 10.1007/s12038-019-9952-9
- https://doi.org/10.1007/s12038-019-9952-9
- Author:
- Zhenghui Tang; Yangxin Fang; Ran Du
- Source:
- Biochemical and biophysical research communications 2019 v.512 no.2 pp. 331-337
- ISSN:
- 0006-291X
- Subject:
- animal ovaries; cell lines; cell proliferation; cyclins; interphase; luciferase; microRNA; ovarian neoplasms; patients; phase transition; therapeutics
- Abstract:
- ... Deregulated expression of microRNAs plays oncogenic or anti-oncogenic roles in various cancers. However, expression of miR-107 was not consistent among several types of cancer, and the effect of miR-107 in ovarian cancer remains unclear. In this study, we found that expression miR-107 was significantly decreased in ovarian cancer patients and in cell lines. Ectopic expression of miR-107 suppressed ...
- DOI:
- 10.1016/j.bbrc.2019.03.009
- https://doi.org/10.1016/j.bbrc.2019.03.009
- Author:
- Yan Jia; Ruixin Lin; Hongjuan Jin; Lihui Si; Wenwen Jian; Qing Yu; Shuli Yang
- Source:
- Biochimie 2019 v.160 pp. 193-199
- ISSN:
- 0300-9084
- Subject:
- adverse effects; animal ovaries; apoptosis; autophagy; cell lines; cell proliferation; drug resistance; drug therapy; humans; luciferase; microRNA; mortality; neoplasm cells; ovarian neoplasms; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; women
- Abstract:
- ... Ovarian cancer is one the prevalent cancers in women and is responsible for 5% of all the cancer related mortalities in women. Owing to late diagnosis, frequent relapses, side effects of chemotherapy, development of drug resistance, there is pressing need to screen out novel and effective treatment options. Accumulating evidences suggest that miRNAs may prove essential therapeutic targets for the ...
- DOI:
- 10.1016/j.biochi.2019.03.011
- https://doi.org/10.1016/j.biochi.2019.03.011
- Author:
- Mohsen Karbalaei Zadeh Babaki; Mahboubeh Taghiabadi; Saman Soleimanpour; Masoud Saleh Moghadam; Arman Mosavat; Abbas Ali Amini; Ali Mohammadi; Seyed Abdolrahim Rezaee
- Source:
- Microbial pathogenesis 2019 v.129 pp. 68-73
- ISSN:
- 0882-4010
- Subject:
- Chinese hamsters; Mycobacterium tuberculosis; Western blotting; adjuvants; agarose; animal ovaries; antigen presentation; antigen-presenting cells; cell lines; fluorescent antibody technique; genes; humans; immune response; immunodominant epitopes; immunoglobulin G; polymerase chain reaction; recombinant fusion proteins; sequence analysis; subunit vaccines; tuberculosis
- Abstract:
- ... Introducing an effective vaccine for tuberculosis (TB) is an urgent need. Mycobacterium tuberculosis (Mtb) Ag85 complex is suggested for making protective immunodominant antigens for design and development of novel TB vaccine. In the present study, a pDR2EF1-Fcγ1 vector has been used to make Ag85B:hFcγ1 recombinant fusion protein. Briefly, specific XbaI and NotI site incorporated primers were used ...
- DOI:
- 10.1016/j.micpath.2019.01.045
- https://doi.org/10.1016/j.micpath.2019.01.045
- Author:
- Kevin J. Pollard; Jill M. Daniel
- Source:
- Molecular and cellular endocrinology 2019 v.490 pp. 68-79
- ISSN:
- 0303-7207
- Subject:
- animal ovaries; cell communication; cell lines; cognition; estrogen receptors; estrogens; insulin-like growth factor I; ligands; mitogen-activated protein kinase; phosphatidylinositol 3-kinase; transcription (genetics); women
- Abstract:
- ... Non-canonical mechanisms of estrogen receptor activation may continue to support women's cognitive health long after cessation of ovarian function. These mechanisms of estrogen receptor activation may include ligand-dependent actions via locally synthesized neuroestrogens and ligand-independent actions via growth factor-dependent activation of intracellular kinase cascades. We tested the hypothesi ...
- DOI:
- 10.1016/j.mce.2019.04.007
- PubMed:
- 30986444
- PubMed Central:
- PMC6520186
- https://doi.org/10.1016/j.mce.2019.04.007
- Author:
- Shangzhong Li; Seong Won Cha; Kelly Heffner; Deniz Baycin Hizal; Michael A. Bowen; Raghothama Chaerkady; Robert N. Cole; Vijay Tejwani; Prashant Kaushik; Michael Henry; Paula Meleady; Susan T. Sharfstein; Michael J. Betenbaugh; Vineet Bafna; Nathan E. Lewis
- Source:
- Journal of proteome research 2019 v.18 no.6 pp. 2433-2445
- ISSN:
- 1535-3907
- Subject:
- Chinese hamsters; Retroviridae; animal ovaries; cell lines; engineering; genes; mutation; phenotype; prediction; proteins; proteome; proteomics; sequence analysis; translation (genetics)
- Abstract:
- ... A high-quality genome annotation greatly facilitates successful cell line engineering. Standard draft genome annotation pipelines are based largely on de novo gene prediction, homology, and RNA-Seq data. However, draft annotations can suffer from incorrect predictions of translated sequence, inaccurate splice isoforms, and missing genes. Here, we generated a draft annotation for the newly assemble ...
- DOI:
- 10.1021/acs.jproteome.8b00935
- PubMed:
- 31020842
- PubMed Central:
- PMC6571120
- https://doi.org/10.1021/acs.jproteome.8b00935
- Author:
- Adeline Poulain; Alaka Mullick; Bernard Massie; Yves Durocher
- Source:
- Journal of biotechnology 2019 v.296 pp. 32-41
- ISSN:
- 0168-1656
- Subject:
- Chinese hamsters; animal ovaries; cell death; cell lines; clones; gene expression; gene expression regulation; genes; monoclonal antibodies; protein synthesis; recombinant proteins; transfection
- Abstract:
- ... Chinese hamster ovary (CHO) cells are the most widely used mammalian host for industrial-scale production of monoclonal antibodies (mAbs) and other protein biologics. Isolation of rare high-producing CHO cell lines from heterogeneous populations of stable transfectants is a daunting task and delays the process of manufacturing of novel biologics. A variety of factors that contribute to the low fre ...
- DOI:
- 10.1016/j.jbiotec.2019.03.009
- https://doi.org/10.1016/j.jbiotec.2019.03.009
- Author:
- Masayoshi Onitsuka; Yukinori Kadoya; Takeshi Omasa
- Source:
- Journal of bioscience and bioengineering 2019 v.127 no.6 pp. 752-757
- ISSN:
- 1389-1723
- Subject:
- Chinese hamsters; animal ovaries; antibodies; bioreactors; byproducts; cell culture; cell lines; cell viability; gel chromatography; immunogenicity; immunoglobulin G; manufacturing; quality control; secretion; therapeutics
- Abstract:
- ... Aggregation of therapeutic antibodies is one of the most important issues to be resolved in manufacturing processes because of reduced efficacy and immunogenicity. Despite aggregation studies in vitro, little is known about the aggregation mechanism in cell culture processes. In this study, we investigated the process of aggregate formation of IgG1 antibodies during the culture of Chinese hamster ...
- DOI:
- 10.1016/j.jbiosc.2018.11.015
- https://doi.org/10.1016/j.jbiosc.2018.11.015
- Author:
- Aneta Rogalska; Arkadiusz Gajek; Agnieszka Marczak
- Source:
- Phytomedicine 2019 v.61 pp. 152847
- ISSN:
- 0944-7113
- Subject:
- P-glycoproteins; acridine orange; adenocarcinoma; animal ovaries; antibodies; apoptosis; autophagy; caspase-3; cell lines; cell viability; confocal microscopy; death; dose response; drug resistance; drug therapy; drugs; enzyme-linked immunosorbent assay; fluorescence; fluorescent antibody technique; humans; microtubules; neoplasm cells; ovarian neoplasms; paclitaxel; staining; toxicity; tubulin
- Abstract:
- ... Epothilones are microtubule-targeting agents that induce death in a variety of cancer cell types. Here, we focus on the cellular and molecular mechanisms underlying epothilone A (Epo A) and epothilone B (Epo B)-induced autophagy and apoptosis in ovarian cancer cells, compared to the actions of the widely used clinical chemotherapy drug paclitaxel (PTX).Autophagy was examined in two cell lines, SKO ...
- DOI:
- 10.1016/j.phymed.2019.152847
- https://doi.org/10.1016/j.phymed.2019.152847
- Author:
- Peng-Cheng LIN; Lin-Lin JI; Xiang-Jian ZHONG; Jin-Jie LI; Xin WANG; Xiao-Ya SHANG; Sheng LIN
- Source:
- Chinese journal of natural medicines 2019 v.17 no.1 pp. 22-26
- ISSN:
- 1875-5364
- Subject:
- Cirsium arvense; Oriental traditional medicine; animal ovaries; antineoplastic activity; cell lines; chemical structure; colorectal neoplasms; cytotoxicity; food plants; humans; inhibitory concentration 50; ovarian neoplasms; secretion; spectral analysis; triterpenoids; tumor necrosis factor-alpha
- Abstract:
- ... Guided by TNF-α secretion inhibitory activity assay, four taraxastane-type triterpenoids, including two new ones, 22-oxo-20-taraxasten-3β, 30-diol (1) and 22α-hydroxy-20-taraxasten-30β, 30-triol (2), have been obtained from an active fraction of the petroleum ether-soluble extract of the the medicinal and edible plant Cirsium setosum. Their structures were elucidated by spectroscopic data and CD d ...
- DOI:
- 10.1016/S1875-5364(19)30005-6
- https://doi.org/10.1016/S1875-5364(19)30005-6
- Author:
- Inês Santos; Cristiano Ramos; Cindy Mendes; Catarina O. Sequeira; Catarina S. Tomé; Dalila G.H. Fernandes; Pedro Mota; Rita F. Pires; Donato Urso; Ana Hipólito; Alexandra M.M. Antunes; João B. Vicente; Sofia A. Pereira; Vasco D. B. Bonifácio; Sofia C. Nunes; Jacinta Serpa
- Source:
- Nutrients 2019 v.11 no.10 pp. -
- ISSN:
- 2072-6643
- Subject:
- animal ovaries; antiporters; cell lines; chrysin; cystathionine; cystathionine beta-synthase; cysteine; cystine; cytotoxicity; death; dendrimers; drugs; glutamic acid; glutathione; hydrogen sulfide; metabolism; nanoparticles; neoplasm cells; ovarian neoplasms; oxidative stress; prognosis; reactive oxygen species; selenium
- Abstract:
- ... Ovarian cancer is the main cause of death from gynecological cancer, with its poor prognosis mainly related to late diagnosis and chemoresistance (acquired or intrinsic) to conventional alkylating and reactive oxygen species (ROS)-generating drugs. We and others reported that the availability of cysteine and glutathione (GSH) impacts the mechanisms of resistance to carboplatin in ovarian cancer. D ...
- DOI:
- 10.3390/nu11102523
- PubMed:
- 31635026
- PubMed Central:
- PMC6836284
- https://doi.org/10.3390/nu11102523
- Author:
- Jing Jin; Wei Xiang; Shuang Wu; Min Wang; Meifang Xiao; Ali Deng
- Source:
- Biochemical and biophysical research communications 2019 v.510 no.4 pp. 580-586
- ISSN:
- 0006-291X
- Subject:
- animal models; animal ovaries; antiviral agents; cell culture; cell lines; cisplatin; gene expression regulation; neoplasm cells; ovarian neoplasms; patients; phosphorylation; signal transduction; tissues
- Abstract:
- ... The essential roles of eukaryotic translation initiation factor 4E (eIF4E) have been shown in various cancers, including ovarian cancer. In this work, we demonstrate that eIF4E inhibition in ovarian cancer can be achieved by ribavirin, a FDA-approved antiviral drug. We show that ribavirin at clinically relevant doses significantly inhibits growth and survival in multiple ovarian cancer cell lines, ...
- DOI:
- 10.1016/j.bbrc.2019.01.117
- https://doi.org/10.1016/j.bbrc.2019.01.117
- Author:
- Piyapat Pin-on; Chatchawit Aporntewan; Jirattha Siriluksana; Narumol Bhummaphan; Pithi Chanvorachote; Apiwat Mutirangura
- Source:
- Gene 2019 v.699 pp. 54-61
- ISSN:
- 0378-1119
- Subject:
- DNA; adrenal glands; animal ovaries; bladder; blood cells; brain; breasts; cell lines; cell proliferation; cervix; colon; epigenetics; essential genes; gene expression; gene expression regulation; head; humans; kidneys; liver; lung neoplasms; lungs; microsatellite repeats; muscles; neck; neoplasm cells; phenotype; proteins; regulatory sequences; tandem repeat sequences; transcription (genetics); transfection; vulva
- Abstract:
- ... Epigenetic regulatory changes alter the gene regulation function of DNA repeat elements in cancer and consequently promote malignant phenotypes. Some short tandem repeat sequences, distributed throughout the human genome, can play a role as cis-regulatory elements of the genes. Distributions of tandem long (≥10) and short (<10) A-T repeats in the genome are different depending on gene functions. L ...
- DOI:
- 10.1016/j.gene.2019.03.010
- https://doi.org/10.1016/j.gene.2019.03.010
- Author:
- Elizabeth C. Rodrigues; Patricia Mörking; Jaqueline O. Rosa; Bruno A. A. Romagnoli; Beatriz G. Guimarães; Priscila M. Hiraiwa; Amanda Klinke; Alessandra M. de Aguiar; Crisciele Kuligovski; Samuel Goldenberg; Augusto S. P. Ramos
- Source:
- Parasitology 2019 v.146 no.11 pp. 1379-1386
- ISSN:
- 1469-8161
- Subject:
- Chagas disease; Chinese hamsters; Golgi apparatus; Trypanosoma cruzi; amastigotes; animal ovaries; biosynthesis; cell lines; endoplasmic reticulum; etiological agents; galactose; genes; glucose transporters; glycoconjugates; glycosylation; heterologous gene expression; lipids; messenger RNA; metabolites; models; mutants; pathogenicity
- Abstract:
- ... Survival and infectivity of trypanosomatids rely on cell-surface and secreted glycoconjugates, many of which contain a variable number of galactose residues. Incorporation of galactose to proteins and lipids occurs along the secretory pathway from UDP-galactose (UDP-Gal). Before being used in glycosylation reactions, however, this activated sugar donor must first be transported across the endoplas ...
- DOI:
- 10.1017/S0031182019000738
- https://doi.org/10.1017/S0031182019000738
- Author:
- Qixuan Guo; Ling Wang; Linfei Zhu; Xinxin Lu; Yongxi Song; Jingxu Sun; Zhonghua Wu; Jinxin Shi; Zhenning Wang; Xin Zhou
- Source:
- Gene 2019 v.713 pp. 143969
- ISSN:
- 0378-1119
- Subject:
- Western blotting; animal ovaries; beta catenin; carcinogenesis; cell lines; metastasis; microarray technology; non-coding RNA; ovarian neoplasms; patients; prognosis; quantitative polymerase chain reaction; tissue repair; tissues
- Abstract:
- ... Ovarian cancer (OvCa) is one of the most lethal gynecologic malignancies worldwide. Pelvic and abdominal metastasis is a leading cause for the poor prognosis of OvCa patients. The relationship between long non-coding RNAs (lncRNAs) and OvCa remains unclear. Identifying key lncRNAs related with OvCa metastasis is crucial for research on the mechanism of OvCa metastasis. This study was designed to i ...
- DOI:
- 10.1016/j.gene.2019.143969
- https://doi.org/10.1016/j.gene.2019.143969
- Author:
- Kieran R. Campbell; Adi Steif; Emma Laks; Hans Zahn; Daniel Lai; Andrew McPherson; Hossein Farahani; Farhia Kabeer; Ciara O’Flanagan; Justina Biele; Jazmine Brimhall; Beixi Wang; Pascale Walters; IMAXT Consortium; Alexandre Bouchard-Côté; Samuel Aparicio; Sohrab P. Shah
- Source:
- Genome biology 2019 v.20 no.1 pp. 54
- ISSN:
- 1474-760X
- Subject:
- DNA; RNA; animal ovaries; breast neoplasms; cell lines; clones; gene expression; humans; neoplasm cells; ovarian neoplasms
- Abstract:
- ... Measuring gene expression of tumor clones at single-cell resolution links functional consequences to somatic alterations. Without scalable methods to simultaneously assay DNA and RNA from the same single cell, parallel single-cell DNA and RNA measurements from independent cell populations must be mapped for genome-transcriptome association. We present clonealign, which assigns gene expression stat ...
- DOI:
- 10.1186/s13059-019-1645-z
- PubMed:
- 30866997
- PubMed Central:
- PMC6417140
- https://doi.org/10.1186/s13059-019-1645-z
- Author:
- Hussain Dahodwala; Kelvin H Lee
- Source:
- Current opinion in biotechnology 2019 v.60 pp. 128-137
- ISSN:
- 0958-1669
- Subject:
- Chinese hamsters; animal ovaries; cell lines; genetic engineering; genome; genomics; plasticity; prediction; product quality; recombinant proteins
- Abstract:
- ... Chinese hamster ovary (CHO) cell-based bioproduction of recombinant proteins can now routinely achieve >5 g/L titers in fed-batches. This progress is partly due to the rapid adaptability of CHO cells to various genetic manipulations and changing process conditions. An inherently plastic genome allows for this adaptability; however, it also gives CHO cells the propensity for genomic rearrangements. ...
- DOI:
- 10.1016/j.copbio.2019.01.011
- https://doi.org/10.1016/j.copbio.2019.01.011
- Author:
- Sha Sha; Seongkyu Yoon
- Source:
- Process biochemistry 2019 v.81 pp. 165-174
- ISSN:
- 1359-5113
- Subject:
- Chinese hamsters; animal ovaries; cell culture; cell lines; feeds; galactose; glucose; glycosylation; monoclonal antibodies; uridine diphosphate
- Abstract:
- ... Monoclonal antibodies (mAbs) produced by Chinese hamster ovary (CHO) cells commonly generate bi-antennary N-linked glycan that is attached with none to one galactose at each antenna. This process is called galactosylation and is dependent on cell lines and cell culture processes. Intracellular uridine diphosphate galactose (UDP-Gal) is the galactosylation precursor and its abundance is modulated b ...
- DOI:
- 10.1016/j.procbio.2019.03.020
- https://doi.org/10.1016/j.procbio.2019.03.020
- Author:
- Mingxia Lu; Claire E. Henry; Haiwang Lai; Yee Yee Khine; Caroline E. Ford; Martina H. Stenzel
- Source:
- Biomaterials science 2019 v.7 no.4 pp. 1652-1660
- ISSN:
- 2047-4849
- Subject:
- adhesion; animal ovaries; cell adhesion; cell lines; drug therapy; drugs; fructose; metastasis; micelles; models; nanoparticles; neoplasm cells; omentum; ovarian neoplasms; p-cymene; patients; relapse; ruthenium; toxicity; women
- Abstract:
- ... Introduction: Ovarian cancer is often diagnosed at a late stage, when disease has spread to extra-pelvic regions such as the omentum. There are limited treatment options available for women with extensive disease and tumours often relapse after current chemotherapy regimens. Therefore, novel drugs should be investigated for the treatment of ovarian cancer. A 3D organotypic model of ovarian cancer ...
- DOI:
- 10.1039/c8bm01326h
- https://doi.org/10.1039/c8bm01326h
- Author:
- Amirhossein Bahreyni; Mona Alibolandi; Mohammad Ramezani; Atefeh Sarafan Sadeghi; Khalil Abnous; Seyed Mohammad Taghdisi
- Source:
- Colloids and surfaces 2019 v.175 pp. 231-238
- ISSN:
- 0927-7765
- Subject:
- Chinese hamsters; adverse effects; animal ovaries; breast neoplasms; cell lines; cell viability; chemical bonding; colloids; colorectal neoplasms; drug therapy; epirubicin; humans; mice; nanoparticles; neoplasm cells; oligonucleotides; polymers; synergism; toxicity
- Abstract:
- ... Conventional chemotherapy suffers from several drawbacks, including toxic side effects together with the development of resistance to the chemical agents. Therefore, exploring alternative therapeutic approaches as well as developing targeted delivery systems are in demand. Oligonucleotide-based therapy has emerged as a promising and alternative procedure for treating malignancies involving gene-re ...
- DOI:
- 10.1016/j.colsurfb.2018.12.006
- https://doi.org/10.1016/j.colsurfb.2018.12.006
- Author:
- Qiong Liu; Ying Zhao; Hui Xing; Lin Li; Rongxia Li; Jie Dai; Quan Li; Shanshan Fang
- Source:
- Gene 2019 v.689 pp. 124-130
- ISSN:
- 0378-1119
- Subject:
- animal ovaries; apoptosis; bioactive properties; cell lines; disease course; drug therapy; gene expression regulation; gene overexpression; genes; messenger RNA; neoplasm cells; ovarian neoplasms; protein content; recombinant proteins; small interfering RNA; tissues
- Abstract:
- ... Aberrant activation of the Wnt/β-catenin has been shown to promote progression in various cancers, including ovarian cancer. However, the molecular mechanisms involved in Wnt/β-catenin activation are not well elucidated. In the work, we identify that R-spondin 1 is an upstream regulator in Wnt/β-catenin pathway to promote growth, survival and migration in ovarian cancer cells. We observe the upreg ...
- DOI:
- 10.1016/j.gene.2018.11.098
- https://doi.org/10.1016/j.gene.2018.11.098