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You searched for: Publication Year 2019 Remove constraint Publication Year: 2019 Subject cell lines Remove constraint Subject: cell lines
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1. (-)-Epigallocatechin-3-gallate and hydroxytyrosol improved antioxidative and anti-inflammatory responses in bovine mammary epithelial cells

Author:
L. Basiricò; P. Morera; D. Dipasquale; R. Bernini; L. Santi; A. Romani; N. Lacetera; U. Bernabucci
Source:
Animal 2019 v.13 no.12 pp. 2847-2856
ISSN:
1751-732X
Subject:
anti-inflammatory activity; antioxidant activity; biomarkers; cell lines; dairy cattle; epigallocatechin gallate; epithelial cells; epithelium; feed supplements; gene expression; glutathione; green tea; hydrogen peroxide; inflammation; interleukin-10; interleukin-6; ligases; lipopolysaccharides; malondialdehyde; mammary glands; messenger RNA; olive oil; oxidative stress; parturition; phenol
Abstract:
... (-)-Epigallocatechin-3-gallate (EGCG), the major phenolic compound of green tea, and hydroxytyrosol (HTyr), a phenol found in olive oil, have received attention due to their wide-ranging health benefits. To date, there are no studies that report their effect in bovine mammary gland. Therefore, the aim of this study was to evaluate the anti-oxidative and anti-inflammatory effects of EGCG and HTyr i ...
DOI:
10.1017/S1751731119001356

2. (Cyto)genomic and epigenetic characterization of BICR 10 cell line and three new established primary human head and neck squamous cell carcinoma cultures

Author:
Ilda P. Ribeiro; Joana M. Rodrigues; Alexandra Mascarenhas; Vanessa Marques; Francisco Caramelo; Maria J. Julião; Thomas Liehr; Joana B. Melo; Isabel M. Carreira
Source:
Genes & genomics 2019 v.41 no.10 pp. 1207-1221
ISSN:
1976-9571
Subject:
GATA transcription factors; cell culture; cell lines; chromosome translocation; chromosomes; comparative genomic hybridization; epigenetics; fluorescence in situ hybridization; genes; genomics; head and neck neoplasms; humans; karyotyping; methylation; models; neoplasm cells; squamous cell carcinoma; therapeutics
Abstract:
... BACKGROUND: Head and neck squamous cell carcinoma cell lines are useful preclinical models to understand the molecular processes underlying the development of such tumors, and to establish targeted therapies. OBJECTIVE: We performed a comprehensive (cyto)genomic and epigenetic characterization of three new established primary human head and neck squamous cell carcinoma cultures and an established, ...
DOI:
10.1007/s13258-019-00850-6

3. (R)‐Tonkafuranone and related compounds: Improved synthesis, stereochemical purity in nature, and bioactivities of the pure enantiomers

Author:
Matthias Hoffmann; Jean‐Pierre Mufusama Koy Sita; Carolin Kleider; Sandra Gunesch; Bruno Rothmund; Leane Lehmann; Gerhard Bringmann; Michael Decker
Source:
Flavour and fragrance journal 2019 v.34 no.5 pp. 329-338
ISSN:
0882-5734
Subject:
Bursera; Lewis bases; cell lines; condensation reactions; cytotoxicity; enantiomers; flavor compounds; high performance liquid chromatography; lactones; mice; moieties; mutagens; patulin; purification methods; stereochemistry
Abstract:
... Dehydromenthofurolactone (tonkafuranone) and other bicyclic lactones are common flavour compounds and can be found in a variety of plants. Herein, we present an improved synthesis of the natural product and a series of close analogues, in combination with efficient purification methods for simple preparation from cheap and easily accessible chemicals. A two‐step, one‐pot, reaction procedure, consi ...
DOI:
10.1002/ffj.3513

4. (−)-Oleocanthal Combined with Lapatinib Treatment Synergized against HER-2 Positive Breast Cancer In Vitro and In Vivo

Author:
Abu Bakar Siddique; Hassan Y. Ebrahim; Mohamed R. Akl; Nehad M. Ayoub; Amira A. Goda; Mohamed M. Mohyeldin; Suresh K. Nagumalli; Wael M. Hananeh; Yong-Yu Liu; Sharon A. Meyer; Khalid A. El Sayed
Source:
Nutrients 2019 v.11 no.2 pp. -
ISSN:
2072-6643
Subject:
Western blotting; antibodies; antineoplastic activity; breast neoplasms; breasts; cell lines; enzyme inhibitors; epidermal growth factor receptors; epithelium; erbB-2 receptor; extra-virgin olive oil; mice; models; neoplasm cells; non-specific protein-tyrosine kinase; non-specific serine/threonine protein kinase; therapeutics; tyrosine
Abstract:
... Dysregulation of epidermal growth factor receptor (EGFR)/human epidermal growth factor-2 (HER2) family is a hallmark of aggressive breast cancer. Small-molecule tyrosine kinase inhibitors are among the most effective cancer targeted treatments. (−)-Oleocanthal (OC) is a naturally occurring phenolic secoiridoid lead from extra-virgin olive oil with documented anti-cancer activities via target ...
DOI:
10.3390/nu11020412
PubMed:
30781364
PubMed Central:
PMC6412724

5. 110ᵗʰ Anniversary: Fast and Easy-to-Use Method for Coating Tissue Culture Polystyrene Surfaces with Nonfouling Copolymers To Prevent Cell Adhesion

Author:
Nicolò Manfredini; Ernesto Scibona; Massimo Morbidelli; Davide Moscatelli; Mattia Sponchioni
Source:
Industrial & engineering chemistry process design and development 2019 v.58 no.49 pp. 22290-22298
ISSN:
1520-5045
Subject:
adhesion; adsorption; cell adhesion; cell lines; coatings; composite polymers; hydrophilicity; moieties; polystyrenes; prices; tissue culture
Abstract:
... Synthetic substrates able to prevent the adhesion of cells, commonly referred to as nonfouling surfaces, are useful in a wide range of applications, including the culture of multicellular spheroids and the engineering of complex cell sheets. Nonfouling properties are commonly conferred to surfaces through superhydrophilic polymer-based coatings. At the same time, this type of treatment requires di ...
DOI:
10.1021/acs.iecr.9b05104

6. 12-Epi-9-deacetoxyxenicin, new cytotoxic diterpenoid from a Bornean soft coral, Xenia sp.

Author:
Chin-Soon Phan; Takashi Kamada; Takahiro Ishii; Toshiyuki Hamada; Charles Santhanaraju Vairappan
Source:
Natural product research 2019 v.33 no.6 pp. 808-813
ISSN:
1478-6427
Subject:
Lagenidium; cell lines; corals; cytotoxicity; diterpenoids; hyphae; secondary metabolites; sesquiterpenoids; spectral analysis; Borneo
Abstract:
... One new compound, 12-epi-9-deacetoxyxenicin (1) along with a hydroperoxide product, 12-epi-9-deacetoxy-8-hydroperoxyxenicin (2) and two known sesquiterpenoids (3–4) were isolated from a population of Bornean soft coral Xenia sp. The structures of these secondary metabolites were elucidated based on their spectroscopic data. Compounds 1 and 2 showed cytotoxic activity against ATL cell line, S1T. In ...
DOI:
10.1080/14786419.2017.1410812

7. 128 Next-generation RNA sequencing of horse adipose and endometrial mesenchymal stem cells from the same donors unveils striking differences in their transcriptomic pattern

Author:
F. Navarrete; E. Mellisho; Y. Wang; J. Cabezas; L. L. Rodriguez-Alvarez; A. Navarro; F. Saravia; F. O. Castro
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 189-190
ISSN:
1031-3613
Subject:
adhesion; angiogenesis; biogenesis; cell growth; cell lines; cell proliferation; endometrium; gene expression regulation; gene ontology; gene overexpression; genes; heat; high-throughput nucleotide sequencing; horses; immune response; immune system; interleukin-8; mesenchymal stromal cells; messenger RNA; osteoblasts; phenotype; platelet-derived growth factor; principal component analysis; stem cells; transcriptomics; Chile
Abstract:
... Earlier we successfully isolated and characterised endometrial (eMSC) and adipose (aMSC) mesenchymal stem cells from the same donors. Mesenchymal stem cells share biological traits but display different surface marker phenotype and migration ability. Here we extended our research to their mRNA signature using next-generation sequencing. The RNA from cells (3 biological replicates from each cell ty ...
DOI:
10.1071/RDv31n1Ab128

8. 182 Establishment of expanded potential embryonic stem cell lines from porcine embryos

Author:
M. Nowak-Imialek; X. Gao; P. Liu; H. Niemann
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 215-216
ISSN:
1031-3613
Subject:
GATA transcription factors; cell culture; cell lines; embryonic germ layers; embryonic stem cells; fetus; flow cytometry; genetic engineering; germ cells; karyotyping; medicine; mice; parthenogenesis; reverse transcriptase polymerase chain reaction; sows; tissues; transgenes; trophoblast; tubulin
Abstract:
... The domestic pig is an excellent large animal in biomedical medicine and holds great potential for testing the clinical safety and efficacy of stem cell therapies. Previously, numerous studies reported the derivation of porcine embryonic stem cell (ESC)-like lines, but none of these lines fulfilled the stringent criteria for true pluripotent germline competent ESC. Here, we report the first establ ...
DOI:
10.1071/RDv31n1Ab182

9. 183 Generation of porcine embryonic stem cell lines derived from nuclear transfer embryos reconstructed with induced pluripotent stem cells

Author:
S. Haraguchi; T. Q. Dang-Nguyen; D. Wells; D. Fuchimoto; T. Fukuda; T. Tokunaga
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 216
ISSN:
1031-3613
Subject:
Lentivirus; RNA; X chromosome; alkaline phosphatase; blastocyst; blastomeres; cell lines; embryonic stem cells; enzyme activity; fibroblasts; fluorescence; genes; genetic vectors; green fluorescent protein; immunosuppression; induced pluripotent stem cells; metaphase; methylation; mice; oocytes; polyvinylpyrrolidone; severe combined immunodeficiency; swine; transfection; zygote
Abstract:
... To establish a porcine embryonic stem (ES) cell line that not only maintains self-renewing capacity but also exhibits pluripotency [Haraguchi et al. 2012 J. Reprod. Dev. 58, 707-716], 6 synthetic porcine RNAs (Oct4, Sox2, Klf4, c-Myc, Nanog, and Lin28) were chemically transfected into outgrowth cultured cells derived from the inner cell mass of in vitro-produced porcine embryos. Subsequently, cell ...
DOI:
10.1071/RDv31n1Ab183

10. 190 Effects of endocrine-disrupting chemical on calcium signalling in cardiomyocyte differentiation from mouse embryonic stem cells

Author:
J.-H. Lee; Y.-M. Yoo; E.-M. Jung; C. Ahn; D. N. Tran; S. Y. Park; B. Lee; B.-H. Jeon; T. H. T. Nguyen; E.-B. Jeung
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 219-220
ISSN:
1031-3613
Subject:
Murine leukemia virus; RNA-directed DNA polymerase; amino acids; analysis of variance; antagonists; beta-mercaptoethanol; bisphenol A; blood pressure; calcium channels; calcium signaling; carbon dioxide; cardiomyocytes; cell lines; complementary DNA; culture media; embryogenesis; embryonic stem cells; endocrine-disrupting chemicals; females; fetal bovine serum; fibroblasts; gene expression; genes; leukemia inhibitory factor; males; messenger RNA; mice; mitomycin; multiple comparison test; penicillins; progesterone; progesterone receptors; quantitative polymerase chain reaction; steroid hormones; streptomycin; tissue culture; transient receptor potential vanilloid channels
Abstract:
... Endocrine-disrupting chemicals (EDC) are substances similar to steroid hormones that can disturb normal physiological functions of male and female reproductive organs. Endocrine-disrupting chemicals tend to bind to steroid hormone receptors. Sex steroid hormones modulate calcium signalling in the cardiac muscle in early embryo development. Among the steroid hormones, progesterone (P4) has been rep ...
DOI:
10.1071/RDv31n1Ab190

11. 2,3,5,4′-Tetrahydroxystilbene-2-O-β-d-glucoside attenuates MPP+/MPTP-induced neurotoxicity in vitro and in vivo by restoring the BDNF-TrkB and FGF2-Akt signaling axis and inhibition of apoptosis

Author:
Yun Yu; Xiu-Yuan Lang; Xi-Xi Li; Run-Ze Gu; Qing-Shan Liu; Rongfeng Lan; Xiao-Yan Qin
Source:
Food & function 2019 v.10 no.9 pp. 6009-6019
ISSN:
2042-650X
Subject:
Alzheimer disease; Parkinson disease; Reynoutria multiflora; animal behavior; animal models; apoptosis; atrophy; cell lines; cell viability; dopamine; immunohistochemistry; in vitro studies; ingredients; mice; neurites; neuroprotective effect; neurotoxicity; neurotrophins; signal transduction; tail suspension test
Abstract:
... The major bioactive ingredient THSG of Polygonum multiflorum is well established for its anti-oxidation, anti-aging and anti-inflammation properties. Increasing evidence supports the capacity of THSG to ameliorate the biochemistry of neurotrophins and their downstream signaling axis in mouse models to attenuate neurodegenerative diseases such as Alzheimer's and Parkinson's disease. In this study, ...
DOI:
10.1039/c9fo01309a

12. 20 Generation of myostatin knockout horse embryos using clustered regularly interspaced short palindromic repeats/CRISPR-associated gene 9 and somatic cell nuclear transfer

Author:
G. Vichera; D. Viale; R. Olivera; V. Arnold; A. Grundnig; J. Baston; S. Miriuka; L. Moro
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 136
ISSN:
1031-3613
Subject:
RNA; animal production; blastocyst; cell lines; clones; dose response; enzymes; exons; fibroblasts; fluorescence; genotyping; heterozygosity; homozygosity; horses; loci; muscles; mutation; myostatin; phenotype; plasmids; polymerase chain reaction; puromycin; selection methods; somatic cells; technology; transfection
Abstract:
... Historically, livestock improvement by selective breeding was the principal selection force in animal production and welfare, but the desired phenotype may involve more than 1 generation. Nowadays, new technologies such as CRISPR/Cas9 could overpass these limits and improve animal quality by insertion or modification of the desired genotype. In this work, we aim to knock out the myostatin (MSTN) g ...
DOI:
10.1071/RDv31n1Ab20

13. 201 Testing of single guide RNAs, optimization of transfection, and selection systems for the generation of SRY knockout foetal fibroblast cells

Author:
G. Vans Landschoot; R. J. Bevacqua; R. Fernandez y Martin; F. A. Pereyra-Bonnet; D. F. Salamone
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 225-226
ISSN:
1031-3613
Subject:
RNA; Y chromosome; animal production; assisted reproductive technologies; cattle; cell lines; cutting; electrodes; electroporation; embryo (animal); endangered species; females; fibroblasts; fluorescence microscopy; gene editing; genes; green fluorescent protein; males; plasmids; somatic cells; statistical analysis; transfection; Massachusetts
Abstract:
... Obtaining male and female cells from a male could have important implications for endangered mammalian species and domestic animal production. Achieving this could constitute a proof of concept of the use of assisted reproduction technologies for the conservation of endangered species. In particular, SRY is the principal male sex determinant gene and is found on the short arm of the Y chromosome. ...
DOI:
10.1071/RDv31n1Ab201

14. 206 Towards the generation of transchromosomic goats for the production of fully human immunoglobulin

Author:
Z. Fan; M. Regouski; M. Brandsrud; H. Wu; Y. Liu; Z. Wang; E. Sullivan; I. A. Polejaeva
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 228
ISSN:
1031-3613
Subject:
RNA; adults; cattle; cell lines; chromosomes; fetus; fibroblasts; gene editing; gene targeting; genes; gestation period; goats; humans; immunoglobulins; polyclonal antibodies; pregnancy; somatic cells; ultrasonography
Abstract:
... We have previously reported that inactivation of endogenous immunoglobulin (Ig) genes can enhance the production of human polyclonal antibodies (hpAb) in transchromosomic (Tc) cattle containing a human artificial chromosome (HAC) comprising the entire human Ig gene repertoire. Goats offer the advantages of having a shorter gestation period and growing to adult size much faster than larger ungulate ...
DOI:
10.1071/RDv31n1Ab206

15. 3,4-dimethoxybenzyl isothiocyanate enhances doxorubicin efficacy in LoVoDX doxorubicin-resistant colon cancer and attenuates its toxicity in vivo

Author:
Mateusz Psurski; Beata Filip-Psurska; Monika Cuprych; Joanna Wietrzyk; Józef Oleksyszyn
Source:
Life sciences 2019 v.231 pp. 116530
ISSN:
0024-3205
Subject:
adenocarcinoma; adverse effects; apoptosis; biomarkers; cell lines; colon; colorectal neoplasms; doxorubicin; growth retardation; humans; in vitro studies; inhibitory concentration 50; isothiocyanates; models; oxidative stress; reactive oxygen species; therapeutics; toxicity
Abstract:
... The aim of the study was to evaluate the potential of naturally occurring isothiocyanates and doxorubicin in combined treatment of doxorubicin-resistant colon cancer. Doxorubicin is a cytostatic commonly used to treat many different types of cancer but its usage is often abrogated by severe side-effects and drug-induced resistance. The antiproliferative potential of the combined treatment was anal ...
DOI:
10.1016/j.lfs.2019.06.005

16. 3-Deoxyglucosone interferes with insulin signaling and attenuates insulin action on glucose-induced GLP-1 secretion in the enteroendocrine L cell line STC-1

Author:
Xiudao Song; Liang Zhou; Heng Xu; Fei Wang; Guoqiang Liang; Lurong Zhang; Fei Huang; Guorong Jiang
Source:
Molecular biology reports 2019 v.46 no.5 pp. 4799-4808
ISSN:
0301-4851
Subject:
Western blotting; cell lines; glucagon-like peptide 1; glucose; glucose transporters; homeostasis; insulin; insulin receptors; molecular biology; phosphatidylinositol 3-kinase; phosphorylation; protein synthesis
Abstract:
... Maintenance of glucose homeostasis is reciprocally regulated by insulin and glucagon-like peptide-1 (GLP-1). We previously reported that GLP-1 secretion in response to an oral glucose load was impaired following an administration of 3-deoxyglucosone (3DG), an independent factor associated with the development of pre-diabetes. Here we investigated the effects of 3DG on insulin signaling and insulin ...
DOI:
10.1007/s11033-019-04926-0

17. 3D porous chitosan-alginate scaffold stiffness promotes differential responses in prostate cancer cell lines

Author:
Kailei Xu; Kavya Ganapathy; Thomas Andl; Zi Wang; John A. Copland; Ratna Chakrabarti; Stephen J. Florczyk
Source:
Biomaterials 2019 v.217 pp. 119311
ISSN:
0142-9612
Subject:
biomarkers; bones; cell growth; cell lines; coculture; death; fibroblasts; men; metastasis; mineralization; neoplasm cells; patients; phenotype; prostatic neoplasms; screening; stem cells; tissue engineering
Abstract:
... Prostate cancer (PCa) is a leading cause of death for men worldwide. Most PCa patients die from metastasis and bone is the most common metastatic site. Three dimensional (3D) porous chitosan-alginate (CA) scaffolds were developed for bone tissue engineering and demonstrated for culture of cancer cells and enrichment of cancer stem cells. However, only a single scaffold composition was studied. Thr ...
DOI:
10.1016/j.biomaterials.2019.119311

18. 3′ Uridylation Confers miRNAs with Non-canonical Target Repertoires

Author:
Acong Yang; Xavier Bofill-De Ros; Tie-Juan Shao; Minjie Jiang; Katherine Li; Patricia Villanueva; Lisheng Dai; Shuo Gu
Source:
Molecular cell 2019 v.75 no.3 pp. 511-522.e4
ISSN:
1097-2765
Subject:
adenosine; cell lines; enzymes; humans; microRNA; models; uridine
Abstract:
... Many microRNAs (miRNAs) exist alongside abundant miRNA isoforms (isomiRs), most of which arise from post-maturation sequence modifications such as 3′ uridylation. However, the ways in which these sequence modifications affect miRNA function remain poorly understood. Here, using human miR-27a in cell lines as a model, we discovered that a nonfunctional target site unable to base-pair extensively wi ...
DOI:
10.1016/j.molcel.2019.05.014
PubMed:
31178353
PubMed Central:
PMC6688926

19. The 3′UTR of the α6 integrin message regulates localization of α6β4 integrin heterodimers

Author:
Alexandra Woychek; Kristina Kligys; Susan B. Hopkinson; Jonathan C.R. Jones
Source:
Biochemical and biophysical research communications 2019 v.513 no.1 pp. 8-14
ISSN:
0006-291X
Subject:
3' untranslated regions; Adenoviridae; adhesion; cell lines; epithelial cells; extracellular matrix; humans; integrins; keratinocytes; lungs
Abstract:
... The α6β4 integrin heterodimer is an essential component of hemidesmosomes (HDs) and HD-related structures, which adhere epithelial cells to the underlying extracellular matrix. In this study, we focused on the importance of the α6 integrin 3′ untranslated region (UTR) in α6β4 integrin localization. To do so, A549 cells (a type II lung alveolar cell line) and immortalized human epidermal keratinocy ...
DOI:
10.1016/j.bbrc.2019.03.116

20. The 5-HT1A receptor: Signaling to behavior

Author:
Paul R. Albert; Faranak Vahid-Ansari
Source:
Biochimie 2019 v.161 pp. 34-45
ISSN:
0300-9084
Subject:
adenylate cyclase; adults; antidepressants; brain; calcium channels; calcium-calmodulin-dependent protein kinase; cell lines; cell viability; inositols; mitogen-activated protein kinase; neurons; phosphatidylinositol 3-kinase; phospholipase C; potassium channels; protein kinase C; receptor protein-tyrosine kinase; receptors; serotonin; synaptogenesis
Abstract:
... The 5-HT1A receptor is highly expressed both in 5-HT neurons as a presynaptic inhibitory autoreceptor, and in many brain regions innervated by 5-HT as a post-synaptic heteroreceptor. This review examines the signaling of 5-HT1A receptors to regulate 5-HT activity and behavior. Initial findings in heterologous cell systems, neuronal cell lines, neurons, and in vivo show that the 5-HT1A receptor is ...
DOI:
10.1016/j.biochi.2018.10.015

21. 68 Flux analysis of aerobic glycolysis in bovine blastocysts and CT1 cells

Author:
J. Chung; R. Clifford; G. Sriram; C. Keefer
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 159
ISSN:
1031-3613
Subject:
air; alanine; biomass; blastocyst; carbon; carbon dioxide; cattle; cell lines; citrates; energy; fatty acids; fructose; gas chromatography-mass spectrometry; glucose; glycolysis; hexoses; lactic acid; metabolites; models; oxygen; pentose phosphate cycle; pregnancy; pyruvic acid; stable isotopes
Abstract:
... Embryo quality and maternal recognition are crucial for successful initiation of bovine pregnancy. Previous studies have proposed that better quality embryos use aerobic glycolysis to meet a high demand for biomass components. While hexoses are the principal carbon sources that provide energy to glycolysis, little is known about partitioning of hexoses into metabolic pathways or alteration of part ...
DOI:
10.1071/RDv31n1Ab68

22. 79 CRISPR gene editing in bovine zygotes — mutation confirmation by integration of protein expression and DNA sequencing analyses

Author:
B. Daigneault; M. Vilarino; S. Rajput; T. Frum; G. Smith; P. Ross
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 165
ISSN:
1031-3613
Subject:
DNA; agarose; cattle; cell lines; computer software; cost effectiveness; embryo (animal); gels; gene editing; genotype; genotyping; glass; image analysis; immunohistochemistry; in vitro fertilization; mutation; oocytes; parthenogenesis; polymerase chain reaction; protein synthesis; proteomics; storage temperature; zygote
Abstract:
... Confirmation of gene editing in livestock following CRISPR/Cas9 zygotic microinjection is often limited to either protein expression or gene sequencing analyses of unfixed embryos, but not both, due to the challenges of extracting DNA from fixed samples suitable for genotyping. Bovine embryos have been derived from gene-edited cell lines to screen for mutations followed by nuclear transfer, but th ...
DOI:
10.1071/RDv31n1Ab79

23. 82 Disruption of p53 in bovine somatic cells affected cloned embryonic development

Author:
K.-M. Kim; S.-J. Lee; S.-Y. Yum; H.-S. Kim; H.-J. Kim; J.-H. Lee; S.-H. Koo; W.-W. Lee; W.-S. Lee; G. Jang
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 166
ISSN:
1031-3613
Subject:
DNA; RNA; analysis of variance; blastocyst; carbon dioxide; cattle; cell lines; clones; computer software; embryogenesis; induced pluripotent stem cells; mutation; neoplasms; oocytes; oxygen; somatic cells; tumor suppressor genes
Abstract:
... The p53 gene is a tumour suppressor gene with many roles in cellular reprogramming, senescence, or cancers. Knockdown of p53 in somatic cells improves generation of induced pluripotent stem cells and developmental competence of cloned embryos. In this study, we hypothesised that disruption of p53 in bovine somatic cells using CRISPR-Cas9 may improve reprogramming and result in increased blastocyst ...
DOI:
10.1071/RDv31n1Ab82

24. 8:2 fluorotelomer alcohol inhibited proliferation and disturbed the expression of pro-inflammatory cytokines and antigen-presenting genes in murine macrophages

Author:
Baida Kong; Xia Wang; Bingnan He; Lai Wei; Jianbo Zhu; Yuanxiang Jin; Zhengwei Fu
Source:
Chemosphere 2019 v.219 pp. 1052-1060
ISSN:
0045-6535
Subject:
alcohols; cell cycle checkpoints; cell lines; cell proliferation; cell viability; gene expression; gene expression regulation; genes; hepatotoxicity; immunotoxicity; industry; interleukin-6; macrophages; messenger RNA; mice; nephrotoxicity; organofluorine compounds; polymers; reactive oxygen species; risk; surfactants; tumor necrosis factor-alpha
Abstract:
... Fluorotelomer alcohols (FTOHs, F(CF₂)ₙCH₂CH₂OH) are members of per- and polyfluoroalkyl substances (PFASs) and are increasingly used in surfactant and polymer industries. FTOHs pose hepatotoxicity, nephrotoxicity and endocrine-disrupting risks. Nevertheless, there is limited research on the immunotoxic effects of FTOHs. In this study, we examined the immunotoxicity of 8:2 FTOH (n = 8) on murine ma ...
DOI:
10.1016/j.chemosphere.2018.12.091

25. N-Eicosapentaenoyl Dopamine, A Conjugate of Dopamine and Eicosapentaenoic Acid (EPA), Exerts Anti-inflammatory Properties in Mouse and Human Macrophages

Author:
Giuseppina Augimeri; Pierluigi Plastina; Giulia Gionfriddo; Daniela Rovito; Cinzia Giordano; Alessia Fazio; Ines Barone; Stefania Catalano; Sebastiano Andò; Daniela Bonofiglio; Jocelijn Meijerink; Renger Witkamp
Source:
Nutrients 2019 v.11 no.9 pp. -
ISSN:
2072-6643
Subject:
amides; amino acids; anti-inflammatory activity; apoptosis; autophagy; cannabinoids; cell lines; chemokine CCL2; chemokine CCL20; docosahexaenoic acid; dopamine; eicosapentaenoic acid; humans; immunomodulators; interleukin-1beta; interleukin-6; lipopolysaccharides; macrophages; metabolites; mice; neoplasms; nitric oxide; omega-3 fatty acids; prostaglandin synthase; risk; transcription (genetics)
Abstract:
... A large body of evidence suggests that dietary n-3 polyunsaturated fatty acids (PUFAs), including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), contribute to a reduced inflammatory tone thereby lowering the risk for several chronic and degenerative diseases. Different mechanisms have been proposed to explain these anti-inflammatory effects, including those involving endocannabinoids ...
DOI:
10.3390/nu11092247
PubMed:
31540502
PubMed Central:
PMC6769480

26. NOP53 as A Candidate Modifier Locus for Familial Non-Medullary Thyroid Cancer

Author:
Aida Orois; Sudheer K. Gara; Mireia Mora; Irene Halperin; Sandra Martínez; Rocio Alfayate; Electron Kebebew; Josep Oriola
Source:
Genes 2019 v.10 no.11 pp. -
ISSN:
2073-4425
Subject:
DNA; cell lines; genes; germ cells; immunohistochemistry; loci; neoplasm cells; protein synthesis; sequence analysis; spouses; thyroid neoplasms
Abstract:
... Nonsyndromic familial non-medullary thyroid cancer (FNMTC) represents 3–9% of thyroid cancers, but the susceptibility gene(s) remain unknown. We designed this multicenter study to analyze families with nonsyndromic FNMTC and identify candidate susceptibility genes. We performed exome sequencing of DNA from four affected individuals from one kindred, with five cases of nonsyndromic FNMTC. Sin ...
DOI:
10.3390/genes10110899
PubMed:
31703244
PubMed Central:
PMC6896177

27. ABCA1 Is Coordinated with ABCB1 in the Arsenic-Resistance of Human Cells

Author:
Tong Zhou; Wanqiang Niu; Zhen Yuan; Shuli Guo; Yang Song; Chunhong Di; Xiaoling Xu; Xiaohua Tan; Lei Yang
Source:
Applied biochemistry and biotechnology 2019 v.187 no.1 pp. 365-377
ISSN:
0273-2289
Subject:
P-glycoproteins; RNA interference; arsenic; cell lines; drug resistance; gene targeting; humans; lipids; plasma membrane; poisoning; resistance genes; survival rate; toxic substances
Abstract:
... Arsenic is one of the most widespread global environmental toxicants associated with endemic poisoning. ATP-binding cassette (ABC) proteins are transmembrane channels that transport and dispose of lipids and metabolic products across the plasma membrane. The majority of ABC family members (including ABCB1 and ABCC1) are reported to play a role in the development of arsenic and drug resistance in m ...
DOI:
10.1007/s12010-018-2800-9

28. ABCG2 aptamer selectively delivers doxorubicin to drug-resistant breast cancer cells

Author:
Shirin Hashemitabar; Rezvan Yazdian-Robati; Maryam Hashemi; Mohammad Ramezani; Khalil Abnous; Fatemeh Kalalinia
Source:
Journal of biosciences 2019 v.44 no.2 pp. 39
ISSN:
0250-5991
Subject:
adverse effects; breast neoplasms; cell lines; cytotoxicity; doxorubicin; drug resistance; drug therapy; flow cytometry; fluorometry; inhibitory concentration 50; neoplasm cells; oligonucleotides
Abstract:
... Chemotherapy is the most widely used treatment for cancer therapy, but its efficacy is limited by the side effects of non-specific cytotoxic drugs. Ligand-based targeting drug-delivery system is a solution to circumvent this issue. In this study, an ABCG2 aptamer–doxorubicin complex was prepared, and its efficacy in targeted drug delivery to mitoxantrone-resistance breast cancer cell line (MCF7/MX ...
DOI:
10.1007/s12038-019-9854-x

29. AFM study: Cell cycle and probe geometry influences nanomechanical characterization of Panc1 cells

Author:
Tanmay Kulkarni; Alex Tam; Debabrata Mukhopadhyay; Santanu Bhattacharya
Source:
Biochimica et biophysica acta 2019 v.1863 no.5 pp. 802-812
ISSN:
0304-4165
Subject:
atomic force microscopy; cell culture; cell lines; geometry; interphase; neoplasm cells; roughness
Abstract:
... Atomic force microscope (AFM) is emerging as an immensely promising tool to study the cellular morphology with a nanometer scale resolution and to analyze nanomechanical properties (NPs) at various physiological conditions. Advancement of AFM technology enables studying living cells and differentiating cancer cell from normal cells based on topography and NPs. Though the trend overlaps from differ ...
DOI:
10.1016/j.bbagen.2019.02.006

30. ALC1 knockdown enhances cisplatin cytotoxicity of esophageal squamous cell carcinoma cells by inhibition of glycolysis through PI3K/Akt pathway

Author:
Fangfang Li; Zhen Zhang; Peng Wang; Penghao Wen; Quanxiao Xu; Yunlong Wang; Ping Pan; Lei Ma
Source:
Life sciences 2019 v.232 pp. 116679
ISSN:
0024-3205
Subject:
2-deoxyglucose; Western blotting; apoptosis; caspase-3; cell lines; cell viability; cisplatin; cytotoxicity; epithelium; esophageal neoplasms; flow cytometry; gene overexpression; glucose; glycolysis; humans; lactic acid; liver neoplasms; messenger RNA; neoplasm cells; oncogenes; pathogenesis; phosphatidylinositol 3-kinase; protein content; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; squamous cell carcinoma
Abstract:
... Amplified in liver cancer 1 gene (ALC1), a recently identified oncogene, was reported to be overexpressed in esophageal cancer cell lines and identified as a target oncogene in esophageal cancer pathogenesis. However, little literature is available to illustrate its significance in cisplatin resistance of esophageal squamous cell carcinoma (ESCC) cells. The aim of the current study was to investig ...
DOI:
10.1016/j.lfs.2019.116679

31. ALK4-SMAD2/3-SMAD4 signaling mediates the activin A-induced suppression of PTX3 in human granulosa-lutein cells

Author:
Chang Liu; Hsun-Ming Chang; Yuyin Yi; Ying Fang; Feiyan Zhao; Peter C.K. Leung; Xiaokui Yang
Source:
Molecular and cellular endocrinology 2019 v.493 pp. 110485
ISSN:
0303-7207
Subject:
cell lines; follicular development; granulosa cells; humans; messenger RNA; models; oocytes; ovulation; phosphorylation; protein content; signal transduction; transforming growth factor beta
Abstract:
... As one of the members of the transforming growth factor-β (TGF-β) superfamily, activin A plays an important role in regulating follicular development and oocyte maturation. Pentraxin 3 (PTX3) is the key component that promotes the process of cumulus expansion during mammalian ovulation. At present, the regulation of PTX3 expression in human granulosa cells remains largely unknown. This study aimed ...
DOI:
10.1016/j.mce.2019.110485

32. ALOX12 inhibition sensitizes breast cancer to chemotherapy via AMPK activation and inhibition of lipid synthesis

Author:
Zhen Huang; Longjie Xia; Xiao Zhou; Changyuan Wei; Qinguo Mo
Source:
Biochemical and biophysical research communications 2019 v.514 no.1 pp. 24-30
ISSN:
0006-291X
Subject:
AMP-activated protein kinase; acetyl coenzyme A; apoptosis; arachidonic acid; breast neoplasms; breasts; cell lines; drug therapy; enzyme activity; gain-of-function mutation; messenger RNA; metabolites; neoplasm cells; patients; protein content; tissues
Abstract:
... Arachidonate lipoxygenase12 (Alox12) and its metabolites 12S-hydroxyeicosatetraenoic acid (12S-HETE) have been implicated in influencing tumor transformation and progression. In this study, we have systematically evaluated the expression, function and the downstream effectors of Alox12 in breast cancer using loss- and gain-of-function approaches. We demonstrated that both mRNA and protein levels o ...
DOI:
10.1016/j.bbrc.2019.04.101

33. ALPL regulates the aggressive potential of high grade serous ovarian cancer cells via a non-canonical WNT pathway

Author:
Mu Luo; Lin Zhou; Shi-jie Zhan; Li-juan Cheng; Ruo-nan Li; Hui wang; Bin Liu; Hua Linghu
Source:
Biochemical and biophysical research communications 2019 v.513 no.2 pp. 528-533
ISSN:
0006-291X
Subject:
animal ovaries; cell lines; cell movement; gene ontology; gene overexpression; genes; genetic disorders; histology; ligands; neoplasm cells; ovarian neoplasms; patients; small interfering RNA
Abstract:
... The ALPL gene is linked to hypophosphatasia, a rare genetic disease. Owing to the inverse relationships between ALPL expression and both the International Federation of Gynecology and Obstetrics (FIGO) stages and histological grades assigned to patients with serous ovarian cancer (SOC), this study was designed to explore the role and possible mechanisms of ALPL in cell motility of high grade SOC ( ...
DOI:
10.1016/j.bbrc.2019.04.016

34. ANXA2Tyr23 and FLNASer2152 phosphorylation associate with poor prognosis in hepatic carcinoma revealed by quantitative phosphoproteomics analysis

Author:
Xiaohua Xing; Hui Yuan; Ying Sun; Kun Ke; Xiuqing Dong; Hui Chen; Xiaolong Liu; Bixing Zhao; Aimin Huang
Source:
Journal of proteomics 2019 v.200 pp. 111-122
ISSN:
1874-3919
Subject:
biomarkers; carcinoma; cell lines; hepatoma; immunologic factors; metastasis; nutritional status; patients; phosphoproteins; phosphorylation; prediction; prognosis; proteomics; signal transduction
Abstract:
... Hepatoma is one of the most common malignant tumors, and most patients have very poor prognosis. Early prediction and intervention of the hepatoma recurrence/metastasis are the most effective way to improve the patients' clinical outcomes. Here, we used isobaric tags for relative and absolute quantitation (iTRAQ) based quantitative phospho-proteomics approach to identify biomarkers associated with ...
DOI:
10.1016/j.jprot.2019.03.017

35. ARHGAP17 suppresses tumor progression and up-regulates P21 and P27 expression via inhibiting PI3K/AKT signaling pathway in cervical cancer

Author:
Qisang Guo; Ying Xiong; Yu Song; Keqin Hua; Shujun Gao
Source:
Gene 2019 v.692 pp. 9-16
ISSN:
0378-1119
Subject:
Western blotting; cell lines; cell proliferation; epithelium; guanosinetriphosphatase; humans; immunohistochemistry; models; neoplasm cells; phenotype; phosphorylation; proteins; reverse transcriptase polymerase chain reaction; signal transduction; tight junctions; uterine cervical neoplasms
Abstract:
... ARHGAP17 has long been thought to be involved in the maintenance of tight junction and epithelial barrier. Recently, a few Rho GTPase activating proteins (RhoGAPs) have been identified as tumor suppressors in some human cancers. The present study aimed to explore ARHGAP17 expression in cervical cancer and the possible function in tumor progression. ARHGAP17 expression in cervical cancer cell lines ...
DOI:
10.1016/j.gene.2019.01.004

36. Acacetin‐induced cell apoptosis in head and neck squamous cell carcinoma cells: Evidence for the role of muscarinic M3 receptor

Author:
Fenglin Sun; Duo Li; Cong Wang; Caixia Peng; Hong Zheng; Xiaofeng Wang
Source:
Phytotherapy research 2019 v.33 no.5 pp. 1551-1561
ISSN:
0951-418X
Subject:
antineoplastic activity; apoptosis; calcium; calcium signaling; caspase-3; caspase-9; cell lines; cell viability; cytochrome c; electron microscopy; flavones; flow cytometry; mitochondria; neoplasm cells; small interfering RNA; squamous cell carcinoma
Abstract:
... Aacacetin, a plant flavone has shown antitumor efficacy recently. However, its associated mechanisms are poorly known. We hypothesized that the muscarinic M3 receptor (M₃R), which is highly expressed in some cancer tissue, is related to the antitumor effect of acacetin in head and neck squamous cell carcinoma (HNSCC) cells. Our results showed that 12.5‐ to 200‐μM acacetin inhibited cell viability ...
DOI:
10.1002/ptr.6343

37. Acalypha wilkesiana flowers: Phenolic profiling, cytotoxic activity of their biosynthesized silver nanoparticles and molecular docking study for its constituents as Topoisomerase-I inhibitors

Author:
Mohamed A. El Raey; Ali M. El-Hagrassi; Abeer F. Osman; Khaled M. Darwish; Mahmoud Emam
Source:
Biocatalysis and agricultural biotechnology 2019 v.20 pp. 101243
ISSN:
1878-8181
Subject:
Acalypha wilkesiana; DNA topoisomerase; Fourier transform infrared spectroscopy; active sites; biosynthesis; breast neoplasms; cell lines; computer simulation; cytotoxicity; flowers; inhibitory concentration 50; nanoparticles; nanosilver; neoplasm cells; prostatic neoplasms; silver; surface plasmon resonance; transmission electron microscopy; ultraviolet-visible spectroscopy; zeta potential
Abstract:
... The bio potential behavior of green silver nanoparticles (AgNPs) using Acalypha wilkesiana flowers extract weas evaluated. The AgNPs formed were confirmed using surface plasmon resonance band showed in UV–vis spectrophotometer that characterized with spherical shape in size range from 5 to 45 nm depending on Transmission electron microscopy (TEM) and with good stability (−50.75 mV) depending on Ze ...
DOI:
10.1016/j.bcab.2019.101243

38. Achyranthes aspera Linn. alleviates cerebral ischemia-reperfusion-induced neurocognitive, biochemical, morphological and histological alterations in Wistar rats

Author:
Gollapalle Lakshminarayanashastry Viswanatha; Marikunte V. Venkataranganna; Nunna Bheema Lingeswara Prasad; Hanumanthappa Shylaja
Source:
Journal of ethnopharmacology 2019 v.228 pp. 58-69
ISSN:
0378-8741
Subject:
2,2-diphenyl-1-picrylhydrazyl; Achyranthes aspera; aerial parts; antioxidants; apigenin; brain; brain damage; carboxymethylcellulose; catalase; cell lines; cognition; edema; enzyme activity; epilepsy; gallic acid; glucose; glutathione; histology; histopathology; infarction; ingredients; inhibitory concentration 50; interleukin-10; interleukin-6; ischemia; laboratory animals; leukocytes; lipid peroxidation; liquid chromatography; males; methanol; nitrites; oral administration; oxygen radical absorbance capacity; quercetin; rats; reference standards; roots; stroke; superoxide dismutase; tandem mass spectrometry; traditional medicine; tumor necrosis factor-alpha
Abstract:
... In the traditional system of Indian medicine, the whole plant and roots of Achyranthes aspera L have been extensively used to treat neurological conditions such as epilepsy and stroke by the various ethnic communities of India.The present study was aimed to evaluate the cerebroprotective potential of methanol extract of A. aspera aerial parts (MeAA).Initially the MeAA was evaluated for total pheno ...
DOI:
10.1016/j.jep.2018.09.018

39. Actinidia arguta (Hardy Kiwi) Root Extract Exerts Anti-cancer Effects via Mcl-1-Mediated Apoptosis in Cholangiocarcinoma

Author:
Xiangxuan Zhao; Feng Wen; Wei Wang; Zaiming Lu; Qiyong Guo
Source:
Nutrition and cancer 2019 v.71 no.2 pp. 246-256
ISSN:
1532-7914
Subject:
Actinidia arguta; NAD ADP-ribosyltransferase; Western blotting; antineoplastic activity; apoptosis; caspase-3; caspase-8; cell lines; cell viability; ethanol; growth retardation; liver; mice; neoplasms; patients; tissues
Abstract:
... Cholangiocarcinoma (CCA) is a highly aggressive and chemoresistant liver malignancy. Thus, identification of strategies to overcome insensitivity to apoptosis and growth inhibition is a growing focus of research in this malignancy. This study evaluated the potential anti-cancer effects of an ethanol extract from the Actinidia arguta (Hardy Kiwi) root (RAE) on CCA. Our data demonstrated that RAE de ...
DOI:
10.1080/01635581.2018.1557218

40. Activating Mutations in PTPN11 and KRAS in Canine Histiocytic Sarcomas

Author:
Marilia Takada; Lauren A. Smyth; Tuddow Thaiwong; Marlee Richter; Sarah M. Corner; Peter Z. Schall; Matti Kiupel; Vilma Yuzbasiyan-Gurkan
Source:
Genes 2019 v.10 no.7 pp. -
ISSN:
2073-4425
Subject:
Golden Retriever; cell lines; dogs; gain-of-function mutation; genes; humans; lymphoma; missense mutation; mitogen-activated protein kinase; sarcoma
Abstract:
... While the genetic contributions to the predisposition of Bernese mountain dogs (BMDs) to histiocytic sarcoma (HS) remains unclear, some insights into key genetic drivers have been gained. Our group recently reported a mutation in the PTPN11 gene (E76K). We have now identified a second missense mutation in PTPN11 (G503V), and a mutation in KRAS (Q61H) present in HS cell lines. These mutations are a ...
DOI:
10.3390/genes10070505
PubMed:
31277422
PubMed Central:
PMC6678586

41. Activation of HO-1 protects placental cells function in oxidative stress via regulating ZO-1/occludin

Author:
Jing Li; Jun Zhou; Baogang Tian; Yijing Chu; Ning Zhang; Xiaoyu Hu; Xin Wan; Yuanhua Ye
Source:
Biochemical and biophysical research communications 2019 v.511 no.4 pp. 903-909
ISSN:
0006-291X
Subject:
cell lines; heme oxygenase (biliverdin-producing); human umbilical vein endothelial cells; humans; hydrogen peroxide; models; occludins; oxidative stress; pathogenesis; pre-eclampsia; small interfering RNA; tight junctions; trophoblast; viruses
Abstract:
... We previously confirmed that Nuclear factor erythroid 2-related factor-2 (Nrf2) and heme oxygenase (HO-1) play synergistic roles in the pathogenesis of preeclampsia. To further explore the function of HO-1 in the pathogenesis of preeclampsia, we established oxidative stress models respectively with human first-trimester trophoblast/simian virus (HTR8/SVneo) and human umbilical vein endothelial cel ...
DOI:
10.1016/j.bbrc.2019.02.144

42. Activation of Notch1 signaling by HTLV-1 Tax promotes proliferation of adult T-cell leukemia cells

Author:
Wenzhao Cheng; Tingjin Zheng; Yong Wang; Kun Cai; Wencai Wu; Tiejun Zhao; Ruian Xu
Source:
Biochemical and biophysical research communications 2019 v.512 no.3 pp. 598-603
ISSN:
0006-291X
Subject:
Primate T-lymphotropic virus 1; T-lymphocytes; adults; cell lines; cell proliferation; half life; leukemia; neoplasm cells
Abstract:
... Human T-cell leukemia virus 1 (HTLV-1), an oncogenic retrovirus, and Notch1 signaling, implicated in tumor formation and progression, are both associated with the development of adult T-cell leukemia (ATL). Here we explored the possibility of a mechanistic link between the two. We observed that the expression of Notch intracellular domain (NICD) was elevated in HTLV-1 infected cell lines. Knocking ...
DOI:
10.1016/j.bbrc.2019.03.094

43. Activation of Nrf2 by costunolide provides neuroprotective effect in PC12 cells

Author:
Shoujiao Peng; Yanan Hou; Juan Yao; Jianguo Fang
Source:
Food & function 2019 v.10 no.7 pp. 4143-4152
ISSN:
2042-650X
Subject:
Inula helenium; antioxidant genes; cell lines; gene expression regulation; homeostasis; lactones; neurodegenerative diseases; neuroprotective effect; oxidative stress; oxidative toxicity; pathogenesis; rats; reactive oxygen species; sesquiterpenoids; signal transduction; thiols; transcription factors
Abstract:
... Costunolide (COS) is a natural sesquiterpene lactone originally isolated from Inula helenium (Compositae). Although COS is known for its multiple pharmacological activities, neuroprotection of COS has not been fully elucidated. Increasing evidence demonstrates that oxidative stress is strongly associated with the progression and pathogenesis of neurodegenerative diseases. As NF-E2 related factor 2 ...
DOI:
10.1039/c8fo02249f

44. Activation of vitamin D receptor attenuates high glucose-induced cellular injury partially dependent on CYP2J5 in murine renal tubule epithelial cell

Author:
Yan Liu; Liu Li; Bin Yi; Zhao-Xin Hu; Ai-Mei Li; Cheng Yang; Li Zheng; Hao Zhang
Source:
Life sciences 2019 v.234 pp. 116755
ISSN:
0024-3205
Subject:
animal disease models; apoptosis; arachidonic acid; calcitriol receptors; cell lines; cytochrome P-450; diabetic nephropathy; epithelial cells; glucose; lactate dehydrogenase; metabolites; mice; renal tubules; therapeutics; vitamin D
Abstract:
... Vitamin D and its receptor, vitamin D receptor (VDR), have renoprotection effect against diabetic nephropathy (DN). But the exact mechanism has not been fully elucidated. Epoxyeicosatrienoic acids (EETs) are cytochrome P450 (CYP) epoxygenase-derived metabolites of arachidonic acid, protecting against diabetes and DN. Herein, we hypothesized that activation of VDR attenuated high glucose-induced ce ...
DOI:
10.1016/j.lfs.2019.116755

45. Activity of N-acylneuraminate-9-phosphatase (NANP) is not essential for de novo sialic acid biosynthesis

Author:
Anke P. Willems; Lingbo Sun; Morten Alder Schulz; Weihua Tian; Angel Ashikov; Monique van Scherpenzeel; Esther Hermans; Henrik Clausen; Zhang Yang; Dirk J. Lefeber
Source:
Biochimica et biophysica acta 2019 v.1863 no.10 pp. 1471-1479
ISSN:
0304-4165
Subject:
biosynthesis; cell lines; cell movement; enzyme activity; genes; genetic disorders; glycolipids; glycoproteins; humans; lectins; liquid chromatography; mass spectrometry; metabolic flux analysis; metabolites; phenotype; polysaccharides; sialic acid; transferases
Abstract:
... Sialylation of glycoproteins and glycolipids is important for biological processes such as cellular communication, cell migration and protein function. Biosynthesis of CMP-sialic acid, the essential substrate, comprises five enzymatic steps, involving ManNAc and sialic acid and their phosphorylated forms as intermediates. Genetic diseases in this pathway result in different and tissue-restricted p ...
DOI:
10.1016/j.bbagen.2019.05.011

46. Acute immune stress improves cell resistance to chemical poison damage in SP600125-induced polyploidy of fish cells in vitro

Author:
Yanxiu Mo; Yunpeng Fan; Wen Fu; Wenting Xu; Shujuan Chen; Yuanhui Wen; Shaojun Liu; Liangyue Peng; Yamei Xiao
Source:
Fish & shellfish immunology 2019 v.84 pp. 656-663
ISSN:
1050-4648
Subject:
breeding; carp; cell adhesion; cell lines; cell-mediated immunity; diploidy; gene expression regulation; homeostasis; inflammation; integrins; interleukin-1beta; mitochondria; shellfish; signal transduction; tetraploidy; toxicity; transcriptome; unigenes
Abstract:
... Previous research has indicated that the small compound, SP600125, could induce polyploidy of fish cells, and has established a stable tetraploid cell line from diploid fish cells. In order to explore how fish cells maintain homeostasis under SP600125-stress in vitro, this study investigates impacts of SP600125-stress on intracellular pathways, as well as on regulation of the cellular homeostasis ...
DOI:
10.1016/j.fsi.2018.10.063

47. Acute toxicity and antiproliferative and procoagulant activities of fractions derived from Thymus satureioides of the Moroccan High Atlas

Author:
T. Khouya; M. Ramchoun; A. Hmidani; B. El moualij; S. Amrani; H. Harnafi; M. Benlyas; Y.Filali Zegzouti; E.H. Nazih; K. Ouguerram; C. Alem
Source:
South African journal of botany 2019 v.121 pp. 568-576
ISSN:
0254-6299
Subject:
2,2-diphenyl-1-picrylhydrazyl; Croton; acute toxicity; anti-inflammatory activity; antioxidant activity; blood coagulation; carrageenan; cell lines; cell membranes; cell viability; coagulation; culture media; edema; erythrocytes; high performance liquid chromatography; hydrophobicity; indigenous species; inflammation; leaves; metabolites; methanol; mortality; plants (botany); polyphenols; prothrombin; rats; rosmarinic acid; thromboplastin
Abstract:
... This study aimed to determine the effects of crude extract (CE) and fractions derived from the Moroccan endemic plant Thymus satureioides (T. satureioides) on inflammation, coagulation and viability of a cell cancer line. The crude extract and fractions from the leaves of T. satureioides were analysed using HPLC and examined for their antioxidant capacity using mineral and biological substrates. T ...
DOI:
10.1016/j.sajb.2019.01.005

48. Adaptation of Vero cells to suspension growth for rabies virus production in different serum free media

Author:
Samia Rourou; Meriem Ben Zakkour; Héla Kallel
Source:
Vaccine 2019 v.37 no.47 pp. 6987-6995
ISSN:
0264-410X
Subject:
Rabies lyssavirus; blood serum; humans; rabies; bioreactors; vaccines; manufacturing; magnesium; dissociation; culture flasks; viral load; vaccine development; cell lines
Abstract:
... Vero cells are nowadays widely used in the production of human vaccines. They are considered as one of the most productive and flexible continuous cell lines available for vaccine manufacturing. However, these cells are anchorage dependent, which greatly complicates upstream processing and process scale-up. Moreover, there is a recognized need to reduce the costs of vaccine manufacturing to develo ...
DOI:
10.1016/j.vaccine.2019.05.092

49. Addressing soluble target interference in the development of a functional assay for the detection of neutralizing antibodies against a BCMA-CD3 bispecific antibody

Author:
Ying Wang; Michael Luong; Crisanto Guadiz; Minlei Zhang; Boris Gorovits
Source:
Journal of immunological methods 2019 pp. -
ISSN:
0022-1759
Subject:
B-lymphocytes; T-lymphocytes; antigens; blood serum; cell lines; drugs; enzyme activity; false positive results; immunogenicity; luciferase; mechanism of action; myeloma; neutralizing antibodies; patients
Abstract:
... Proper evaluation of immunogenicity during clinical development of biotherapeutics is a major challenge to bioanalytical scientists, in part due to matrix interference in anti-drug antibody (ADA) and neutralizing antibody (NAB) assays. If not addressed, matrix interference could confound the immunogenicity assessment of a given biotherapeutic in clinical development. To support clinical developmen ...
DOI:
10.1016/j.jim.2019.112642

50. Adhesion mechanisms mediated by probiotics and prebiotics and their potential impact on human health

Author:
Andrea Monteagudo-Mera; Robert A. Rastall; Glenn R. Gibson; Dimitris Charalampopoulos; Afroditi Chatzifragkou
Source:
Applied microbiology and biotechnology 2019 v.103 no.16 pp. 6463-6472
ISSN:
0175-7598
Subject:
absorption barrier; adhesion; anti-infective agents; antibiosis; bacterial adhesion; binding sites; biochemical pathways; cell lines; competitive exclusion; enteropathogens; epithelium; human health; immunomodulators; in vitro studies; intestines; mouth; prebiotics; probiotics; protective effect; receptors; toxins
Abstract:
... Adhesion ability to the host is a classical selection criterion for potential probiotic bacteria that could result in a transient colonisation that would help to promote immunomodulatory effects, as well as stimulate gut barrier and metabolic functions. In addition, probiotic bacteria have a potential protective role against enteropathogens through different mechanisms including production of anti ...
DOI:
10.1007/s00253-019-09978-7
PubMed:
31267231
PubMed Central:
PMC6667406