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You searched for: Publication Year 2019 Remove constraint Publication Year: 2019 Subject term biotin Remove constraint Subject term: biotin
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1. 3D printing with peptide–polymer conjugates for single-step fabrication of spatially functionalized scaffolds

Author:
Paula Camacho; Hafiz Busari; Kelly B. Seims; Peter Schwarzenberg; Hannah L. Dailey; Lesley W. Chow
Source:
Biomaterials science 2019 v.7 no.10 pp. 4237-4247
ISSN:
2047-4849
Subject:
adhesion; azides; biocompatible materials; biodegradability; biotin; cell adhesion; fibroblasts; fluorescent dyes; peptides; polymers; printers; three-dimensional printing
Abstract:
... Biodegradable polymer-based scaffolds are widely used to provide support during early stages of regeneration and can be functionalized with various chemical groups or bioactive cues to promote desired cellular behavior. However, these scaffolds are often modified post-fabrication, which can lead to undesired changes and homogeneously distributed chemistries that fail to mimic the spatial biochemic ...
DOI:
10.1039/c9bm00887j

2. 55 Peri-conceptional undernourishment perturbs offspring sperm methylome

Author:
P. Toschi; E. Capra; D. Anzalone; F. Turri; F. Pizzi; B. Lazzari; A. Stella; P. Ajmone-Marsan; P. Loi
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 153
ISSN:
1031-3613
Subject:
DNA methylation; adults; animal models; biotin; bisulfites; blastocyst; body weight; chondroitin sulfate; chromatin; conception; diet; embryogenesis; epigenetics; flow cytometry; folic acid; gene ontology; genotype; glucagon; inheritance (genetics); ion transport; lambs; malnutrition; maternal exposure; maternal nutrition; metabolism; mothers; nutrient availability; phenotype; potassium; progeny; rams; semen; sequence analysis; sperm motility; spermatozoa
Abstract:
... Whereas an organism’s genotype is relatively static throughout life, the epigenome is highly dynamic and can adapt, or be altered, in response to the external environment such as diet. Intrauterine exposure to nutrient availability can alter the establishment of epigenetic marks, not only in the exposed individuals, but also in their offspring. Inheritance of such an environmentally acquired pheno ...
DOI:
10.1071/RDv31n1Ab55

3. Application of a novel FAM-conjugated activity-based probe to determine cathepsin G activity intracellularly

Author:
Roman Schroeder; Renata Grzywa; Christian Rainer Wirtz; Marcin Sienczyk; Timo Burster
Source:
Analytical biochemistry 2019 pp. -
ISSN:
0003-2697
Subject:
CD4-positive T-lymphocytes; CD8-positive T-lymphocytes; active sites; amino acid sequences; avidin; biotin; cathepsin G; chemical bonding; enzyme activity; flow cytometry; innate immunity; moieties; natural killer cells; phosphonates; serine; signal-to-noise ratio
Abstract:
... Cathepsin G (CatG) is responsible for several distinct immune processes of adaptive and innate immunity depending on extra- or intracellular occurrence of CatG. Recently, we established a method to detect CatG activity at the cell surface of natural killer cells by using the activity-based probe MARS116-Bt in flow cytometry. MARS116-Bt consists of biotin, spacer, amino acid sequence, and a phospho ...
DOI:
10.1016/j.ab.2019.113488

4. Beneficial Effects of n-3 Polyunsaturated Fatty Acids on Offspring’s Pancreas of Gestational Diabetes Rats

Author:
Jinlong Gao; Tao Huang; Jiaomei Li; Xiaofei Guo; Hailong Xiao; Jiaojiao Gu; Jun Tang; Wenwen Cai; Duo Li
Source:
Journal of agricultural and food chemistry 2019 v.67 no.48 pp. 13269-13281
ISSN:
1520-5118
Subject:
acetates; biochemical pathways; biotin; ceramides; gestational diabetes; inflammation; long term effects; metabolites; metabolomics; omega-3 fatty acids; oxidative stress; pancreas; polyunsaturated fatty acids; progeny; rats; telomeres; tocotrienols; valine
Abstract:
... We studied the long-term influence of gestational diabetes mellitus (GDM) on the pancreas of offspring and the effect of omega-3 polyunsaturated fatty acids (n-3 PUFAs) on offspring’s pancreas. GDM offspring were divided into three groups: GDM offspring, n-3 PUFA-adequate-GDM offspring, and n-3 PUFA-deficient GDM offspring. All healthy and GDM offspring were fed up to 11 months old. The pancreas o ...
DOI:
10.1021/acs.jafc.9b05739

5. Bioluminescent aptamer-based solid-phase microassay to detect lung tumor cells in plasma

Author:
Eugenia E. Bashmakova; Vasilisa V. Krasitskaya; Galina S. Zamay; Tatiana N. Zamay; Ludmila A. Frank
Source:
Talanta 2019 v.199 pp. 674-678
ISSN:
0039-9140
Subject:
bioluminescence; biotin; blood; blood sampling; luminescent proteins; lung neoplasms; magnetism; neoplasm cells; nucleotide aptamers; patients
Abstract:
... Two high-affinity DNA aptamers for lung tumor cells were applied as biospecific elements in bioluminescent assay of patient blood. The oligonucleotide complementary to the 5′ end of both aptamers carrying either biotin or Ca2+-regulated photoprotein obelin was used to form a sandwich-type analytical complex on the surfaces of magnetic streptavidin-activated microspherical particles. Clinical blood ...
DOI:
10.1016/j.talanta.2019.03.030

6. Biotin Is Required for the Zinc Homeostasis in the Skin

Author:
Youichi Ogawa; Manao Kinoshita; Takuya Sato; Shinji Shimada; Tatsuyoshi Kawamura
Source:
Nutrients 2019 v.11 no.4 pp. -
ISSN:
2072-6643
Subject:
Langerhans cells; acrodermatitis; adenosine monophosphate; adenosine triphosphate; biotin; blood serum; contact dermatitis; diet; homeostasis; keratinocytes; mice; nutrient deficiencies; patients; zinc
Abstract:
... Patients with biotin deficiency present symptoms that are similar to those in patients with acrodermatitis enteropathica (inherent zinc deficiency). However, the association between biotin and zinc deficiency remains unknown. We have previously shown that epidermal keratinocytes of mice fed zinc-deficient (ZD) diets secreted more adenosine triphosphate (ATP) than those of mice fed zinc-adequate (Z ...
DOI:
10.3390/nu11040919
PubMed:
31022908
PubMed Central:
PMC6520690

7. Biotin and arginine modified hydroxypropyl-β-cyclodextrin nanoparticles as novel drug delivery systems for paclitaxel

Author:
Caixia Yan; Na Liang; Qiang Li; Pengfei Yan; Shaoping Sun
Source:
Carbohydrate polymers 2019 v.216 pp. 129-139
ISSN:
0144-8617
Subject:
X-ray diffraction; antineoplastic activity; arginine; biotin; drug delivery systems; emulsions; encapsulation; endocytosis; evaporation; in vivo studies; moieties; nanoparticles; paclitaxel; solvents; transmission electron microscopy; zeta potential
Abstract:
... A novel biotin and arginine modified hydroxypropyl-β-cyclodextrin (biotin-Arg(pbf)-HP-β-CD) was successfully synthesized. The hydroxyl groups of HP-β-CD on the primary faces were coupled with carboxyl groups of biotin using arginine as the functional spacer. Using biotin-Arg(pbf)-HP-β-CD as the carrier, paclitaxel (PTX)-loaded nanoparticles were developed by modified emulsion solvent evaporation m ...
DOI:
10.1016/j.carbpol.2019.04.024

8. Biotin improves relaxation of rat aortic rings in combination with antihypertensive drugs

Author:
Asdrubal Aguilera-Méndez; Ricardo Espino-García; Zaira Jatziri Toledo-López; Zurisaddai Hernández-Gallegos; Santiago Villafaña-Rauda; Renato Nieto-Aguilar; Deyanira Serrato-Ochoa; Gema Cecilia Manuel-Jacobo
Source:
PharmaNutrition 2019 v.8 pp. 100147
ISSN:
2213-4344
Subject:
antihypertensive agents; antihypertensive effect; biotin; calcium; endothelium; hyperglycemia; hypertension; hypertriglyceridemia; metabolic syndrome; models; phenylephrine; rats; therapeutics; vasoconstriction; vasodilator agents
Abstract:
... Biotin at pharmacological concentrations has beneficial effects on hypertriglyceridaemia, hyperglycaemia and hypertension. We recently reported in a model of metabolic syndrome that biotin has an antihypertensive effect and decreases arterial contraction. In this study, we investigated the effect of biotin (10 nM) on arterial contraction in rat aortic rings with or without an endothelium induced b ...
DOI:
10.1016/j.phanu.2019.100147

9. Biotin interference in immunoassays based on biotin-strept(avidin) chemistry: An emerging threat

Author:
John H.T. Luong; Keith B. Male; Jeremy D. Glennon
Source:
Biotechnology advances 2019 v.37 no.5 pp. 634-641
ISSN:
0734-9750
Subject:
antibodies; antigens; avidin; biomarkers; biosensors; biotin; blood; blood sampling; cardiac output; detection limit; drugs; enzymes; foods; half life; hormones; humans; immunoassays; metabolic diseases; milk; mitochondria; neoplasms; patients; sclerosis
Abstract:
... Biotinylated antibodies/antigens are currently used in many immunoassay formats in clinical settings for diversified analytes and biomarkers to offer high detection selectivity and sensitivity. Biotin cannot be synthesized by mammals and must be taken as an essential supplement. Normal intake of biotin from various foods and milk causes no effect on the streptavidin/biotin-based immunoassays. Howe ...
DOI:
10.1016/j.biotechadv.2019.03.007

10. Biotin-modified bovine serum albumin nanoparticles as a potential drug delivery system for paclitaxel

Author:
Danfeng Wang; Na Liang; Yoshiaki Kawashima; Fude Cui; Pengfei Yan; Shaoping Sun
Source:
Journal of materials science 2019 v.54 no.11 pp. 8613-8626
ISSN:
0022-2461
Subject:
Fourier transform infrared spectroscopy; X-ray diffraction; biotin; bovine serum albumin; cytotoxicity; disulfide bonds; encapsulation; endocytosis; flow cytometry; glutathione; nanocarriers; nanoparticles; pH; paclitaxel; solvents; temperature; toxicity testing; transmission electron microscopy; zeta potential
Abstract:
... This study proposed a novel injectable nanocarrier for paclitaxel (PTX) based on biotin-modified bovine serum albumin (BSA). First, the biotin-modified BSA (biotin-BSA) was successfully synthesized and characterized by FT-IR analysis. Then, the PTX-loaded biotin-BSA nanoparticles (NPs) were prepared by disulfide bond reducing method and stabilized through the formation of intermolecular disulfide ...
DOI:
10.1007/s10853-019-03486-9

11. CD8 T cell-derived perforin aggravates secondary spinal cord injury through destroying the blood-spinal cord barrier

Author:
Zhaoxiang Liu; Hua Zhang; Hong Xia; Baocheng Wang; Renwen Zhang; Qun Zeng; Lingzhi Guo; Kui Shen; BaTa Wang; Yanheng Zhong; Zhizhong Li; Guodong Sun
Source:
Biochemical and biophysical research communications 2019 v.512 no.2 pp. 367-372
ISSN:
0006-291X
Subject:
CD8-positive T-lymphocytes; animal injuries; animal models; biotin; cerebrospinal fluid; cytokines; enzyme-linked immunosorbent assay; flow cytometry; humans; infectious diseases; inflammation; mice; permeability; spinal cord; staining
Abstract:
... Perforin plays an important role in autoimmune and infectious diseases, but its function in immune inflammatory responses after spinal cord injury (SCI) has received insufficient attention. The goal of this study is to determine the influence of perforin after spinal cord injury (SCI) on secondary inflammation. Compared recovery from SCI in perforin knockout (Prf1−/−) and wild-type(WT)mice, WT mic ...
DOI:
10.1016/j.bbrc.2019.03.002

12. Candidatus Krumholzibacterium zodletonense gen. nov., sp nov, the first representative of the candidate phylum Krumholzibacteriota phyl. nov. recovered from an anoxic sulfidic spring using genome resolved metagenomics

Author:
Noha H. Youssef; Ibrahim F. Farag; C. Ryan Hahn; Hasitha Premathilake; Emily Fry; Matthew Hart; Krystal Huffaker; Edward Bird; Jimmre Hambright; Wouter D. Hoff; Mostafa S. Elshahed
Source:
Systematic and applied microbiology 2019 v.42 no.1 pp. 85-93
ISSN:
0723-2020
Subject:
biotin; dipeptides; fimbriae; freshwater; genome; genome assembly; geographical distribution; habitats; lifestyle; lipoic acid; metagenomics; new family; new genus; new order; new species; phylogeny; proline; pyridoxine; sugars; sulfur springs; tripeptides; vitamin B12; Oklahoma
Abstract:
... The accumulation of genomes of uncultured organisms has highlighted the need for devising a taxonomic and nomenclature scheme to validate names and prevent redundancies. We here report on the recovery and analysis of four phylogenetically related genomes recovered from an anoxic sulfide and sulfur-rich spring (Zodletone spring) in southwestern Oklahoma. Phylogenetic analysis based on 120 single co ...
DOI:
10.1016/j.syapm.2018.11.002

13. Characterization of a Hypovirus-Regulated Septin Cdc11 Ortholog, CpSep1, from the Chestnut Blight Fungus Cryphonectria parasitica

Author:
Myeongjin Jo; Kum-Kang So; Yo-Han Ko; Jeesun Chun; Jung-Mi Kim; Dae-Hyuk Kim
Source:
Molecular plant-microbe interactions 2019 v.32 no.3 pp. 286-295
ISSN:
0894-0282
Subject:
Aspergillus nidulans; Castanea; Cryphonectria hypovirus 1; Cryphonectria parasitica; Saccharomyces cerevisiae; apples; bark; biotin; conidia; culture media; fungi; gene expression regulation; genes; germination; growth retardation; hyphae; mutants; mycelium; pigmentation; sorbitol; stems; tannins; virulence
Abstract:
... We identified a protein spot showing downregulation in the presence of Cryphonectria hypovirus 1 and tannic acid supplementation as a septin subunit with the highest homology to the Aspergillus nidulans aspA gene, an ortholog of the Saccharomyces cerevisiae Cdc11 gene. To analyze the functional role of this septin component (CpSep1), we constructed its null mutant and obtained a total of eight CpS ...
DOI:
10.1094/MPMI-07-18-0194-R

14. Characterization of the kinetics and activation thermodynamics of intra- and inter-organism hybrid tetramers of pyruvate carboxylase

Author:
Abdussalam Adina-Zada; Sarawut Jitrapakdee; Paul V. Attwood
Source:
Archives of biochemistry and biophysics 2019 v.665 pp. 87-95
ISSN:
0003-9861
Subject:
Aspergillus nidulans; Rhizobium etli; avidin; bacteria; biotin; carboxylation; catalytic activity; chemical bonding; decarboxylation; fungi; hybrids; models; mutants; pyruvate carboxylase; pyruvic acid; thermodynamics
Abstract:
... In sedimentation velocity experiments, we have been able to detect hybrid Rhizobium etli pyruvate carboxylase tetramers formed between subunits that contain covalently bound biotin and mutant subunits that do not. This was performed by forming complexes of the tetramers with the biotin-binding protein avidin. In addition, we have shown that it is possible to form hybrid tetramers of pyruvate carbo ...
DOI:
10.1016/j.abb.2019.02.018

15. Chemical Synthesis of Atomically Tailored SUMO E2 Conjugating Enzymes for the Formation of Covalently Linked SUMO–E2–E3 Ligase Ternary Complexes

Author:
Yinfeng Zhang; Tsuyoshi Hirota; Keiko Kuwata; Shunsuke Oishi; Subramanian G. Gramani; Jeffrey W. Bode
Source:
Journal of the American Chemical Society 2019 v.141 no.37 pp. 14742-14751
ISSN:
1520-5126
Subject:
biotin; catalytic activity; chemical bonding; moieties; serine; synthesis; synthetic proteins; threonine; ubiquitin; ubiquitin-protein ligase; ultraviolet radiation
Abstract:
... E2 conjugating enzymes are the key catalytic actors in the transfer of ubiquitin, SUMO, and other ubiquitin-like modifiers to their substrate proteins. Their high rates of transfer and promiscuous binding complicate studies of their interactions and binding partners. To access specific, covalently linked conjugates of the SUMO E2 conjugating enzyme Ubc9, we prepared synthetic variants bearing site ...
DOI:
10.1021/jacs.9b06820

16. A Collagen Supplement Improves Skin Hydration, Elasticity, Roughness, and Density: Results of a Randomized, Placebo-Controlled, Blind Study

Author:
Liane Bolke; Gerrit Schlippe; Joachim Gerß; Werner Voss
Source:
Nutrients 2019 v.11 no.10 pp. -
ISSN:
2072-6643
Subject:
acerolas; ascorbic acid; biotin; collagen; dietary supplements; drinking; fruit extracts; health services; peptides; placebos; roughness; silicon; skin (animal); ultrasonography; vitamin E; women; zinc; Germany
Abstract:
... The purpose of this randomized, placebo-controlled, blind study was to investigate the effects of the drinkable nutraceutical ELASTEN® (QUIRIS Healthcare, Gütersloh, Germany) on skin aging and skin health. Drinking ampoules provides a blend of 2.5 g of collagen peptides, acerola fruit extract, vitamin C, zinc, biotin, and a native vitamin E complex. This controlled interventional trial wa ...
DOI:
10.3390/nu11102494
PubMed:
31627309
PubMed Central:
PMC6835901

17. Colloidal SERS Substrate for the Ultrasensitive Detection of Biotinylated Antibodies Based on Near-Field Gradient within the Gap of Au Nanoparticle Dimers

Author:
Pablo A. Mercadal; Ezequiel R. Encina; Eduardo A. Coronado
Source:
Journal of physical chemistry 2019 v.123 no.38 pp. 23577-23585
ISSN:
1932-7455
Subject:
Raman spectroscopy; antibodies; biotin; chemical bonding; chemical species; food science; gliadin; gold; immunoglobulin G; nanogold; nanorods; streptavidin
Abstract:
... Surface-enhanced Raman spectroscopy (SERS) has demonstrated to be a powerful technique for the ultrasensitive detection of different types of analytes and particularly biomolecules, with the rational design of SERS substrates being one of the most relevant issues for the development of effective detection protocols. In this work, a colloidal SERS substrate consisting of a pair of Au nanorods linke ...
DOI:
10.1021/acs.jpcc.9b02974

18. Competitive light-initiated chemiluminescent assay: using 5-α-dihydrotestosterone-BSA as competitive antigen for quantitation of total testosterone in human sera

Author:
Yaqiong Cui; Tiantian She; Hong Zhao; Jiuzhi Li; Liuxu Li; Weizhen Gao; Huiqiang Li
Source:
Analytical and bioanalytical chemistry 2019 v.411 no.3 pp. 745-754
ISSN:
1618-2642
Subject:
antibodies; antigens; biotin; blood serum; bovine serum albumin; chemiluminescence; cross reaction; dihydrotestosterone; energy transfer; humans; photosensitizing agents; streptavidin; testosterone
Abstract:
... This paper described a homogeneous method, light-initiated chemiluminescent assay (LICA), for quantitation of total testosterone in human sera. The assay was bead based and built on a competitive-binding reaction format, in which 5-α-dihydrotestosterone (5-α-DHT) competed with the testosterone in serum samples in binding with biotinylated anti-testosterone antibody. The more testosterone in the se ...
DOI:
10.1007/s00216-018-1496-6

19. DNA ligase photocrosslinked cryogenic column based biotinylation kit for viral hybridization and detection

Author:
Semra Özgün Köse; Cem Öziç; Filiz Yılmaz; Arzu Ersöz; Rıdvan Say
Source:
Process biochemistry 2019 v.84 pp. 213-219
ISSN:
1359-5113
Subject:
Crimean-Congo hemorrhagic fever; adenosine triphosphate; biotin; biotinylation; crosslinking; cryogels; fluorescence; haptens; ligands; ligases; nanoparticles; nucleic acid hybridization; photosensitivity; polymerase chain reaction; quantum dots; ruthenium; single-stranded DNA
Abstract:
... This study describes the development of cryogel columns prepared by ATP having ruthenium hapten cross-linker based photosensitive approach “ANADOLUCA” for the biotinylation of Crimean Congo Hemorrhagic Fever (CCHF) primers through 5′ end as a first. In this process, which does not require ATP ligand, CCHF recognition has been performed using stable and quantum dots (QDs) based avidity nanoparticle ...
DOI:
10.1016/j.procbio.2019.06.011

20. A DNAzyme assay coupled with effective magnetic separation and rolling circle amplification for detection of lead cations with a smartphone camera

Author:
Wei Lu; Caiqin Lin; Jun Yang; Xiaoqi Wang; Bo Yao; Min Wang
Source:
Analytical and bioanalytical chemistry 2019 v.411 no.21 pp. 5383-5391
ISSN:
1618-2642
Subject:
biotin; cameras; cations; ecotoxicology; fluorescence; health services; humans; instrumentation; lakes; lead; magnetic separation; magnetism; mobile telephones; monitoring
Abstract:
... Rapid, portable, and efficient detection of lead cations (Pb²⁺) is of great significance for monitoring and evaluating environmental toxicity and human healthcare. In this work, we developed a simple and low-cost homogenous fluorescence DNAzyme assay for Pb²⁺ determination based on Pb²⁺-dependent cleaving and rolling circle amplification (RCA). DNAzyme and its substrate (5′-biotin) formed double-s ...
DOI:
10.1007/s00216-019-01879-y

21. Development of a Sensitive Chemiluminescence Immunoassay for the Quantification of Folic Acid in Human Serum

Author:
Xiang Chen; Qiyang Zhou; Ting Zhang; ChunXin Wang; Zheng Yu; Hadji Ahamada; Zhonghu Bai; Xuan Huang
Source:
Journal of Analytical Methods in Chemistry (Online) 2019 v.2019 pp. -
ISSN:
2090-8873
Subject:
aminopterin; anemia; behavior disorders; biotin; blood serum; cardiovascular diseases; chemiluminescence immunoassays; cross reaction; diarrhea; equations; folate-binding proteins; folic acid; humans; magnetism; methotrexate; milk; models; neural tube defects; peroxidase; phosphine; pregnant women; streptavidin; thermal stability
Abstract:
... Folic acid (FA) is an important vitamin for human growth, especially for pregnant women. FA deficiency is associated with megaloblastic anemia, neural tube defects, cardiovascular diseases, irritability, diarrhea, and psychiatric disorders. Normally, FA molecules bind to folate-binding protein (FBP) in the serum as complex. Before quantify the FA concentration, a releasing procedure should be cond ...
DOI:
10.1155/2019/5402903
PubMed:
31240147
PubMed Central:
PMC6556278

22. Development of a body fluid identification multiplex via DNA methylation analysis

Author:
Quentin T. Gauthier; Sohee Cho; Justin H. Carmel; Bruce R. McCord
Source:
Electrophoresis 2019 v.40 no.18-19 pp. 2565-2574
ISSN:
0173-0835
Subject:
DNA; DNA methylation; biotin; bisulfites; blood; cluster analysis; cytosine; electrophoresis; epigenetics; epithelium; forensic sciences; guanine; loci; models; polymerase chain reaction; saliva; semen; sequence analysis; uracil
Abstract:
... The goal of this study is to develop an epigenetic multiplex for body fluid identification based on tissue specific DNA methylation. A series of genetic loci capable of discerning the origin of DNA as coming from saliva, blood, vaginal epithelia, or semen were used for this application. The markers – BCAS4, CG06379435, VE_8, and ZC3H12D – were amplified together and then sequenced via pyrosequenci ...
DOI:
10.1002/elps.201900118

23. Development of a nucleic acid lateral flow strip for rapid, visual detection of Nosema bombycis in silkworm eggs

Author:
Zhangshuai He; Qi Ni; Yue Song; Rui Wang; Yunlin Tang; Yujiao Wu; Lulu Liu; Jialing Bao; Jie Chen; Mengxian Long; Junhong Wei; Chunfeng Li; Tian Li; Zeyang Zhou; Guoqing Pan
Source:
Journal of invertebrate pathology 2019 v.164 pp. 59-65
ISSN:
0022-2011
Subject:
DNA primers; Nosema bombycis; agar gel electrophoresis; biotin; detection limit; egg production; eggs; females; financial economics; gold; moths; pathogens; polymerase chain reaction; quarantine; ribosomal DNA; sericulture; silkworms
Abstract:
... Nosema bombycis, the pathogen of silkworm pébrine, causes enormous economic losses to sericulture. As such, quarantine of commercial silkworm eggs represents an important safeguard to the silkworm industry. Here, we established a user-friendly detection system based on a nucleic acid lateral flow strip (NAFLS) that combines polymerase chain reaction (PCR) and a colloidal gold strip. PCR primers we ...
DOI:
10.1016/j.jip.2019.04.004

24. Development of a reverse transcription recombinase polymerase amplification combined with lateral‐flow dipstick assay for avian influenza H9N2 HA gene detection

Author:
Zeng Wang; Pan‐Pan Yang; Yu‐Han Zhang; Kai‐Yue Tian; Chuan‐Zhou Bian; Jun Zhao
Source:
Transboundary and emerging diseases 2019 v.66 no.1 pp. 546-551
ISSN:
1865-1674
Subject:
Influenza A virus; RNA; avian influenza; biotin; chickens; cloaca; emerging diseases; fluorescein; genes; hemagglutinins; isothiocyanates; mortality; pathogens; reverse transcriptase polymerase chain reaction; reverse transcription; secondary infection; temperature; viruses; wild birds
Abstract:
... H9N2 avian influenza viruses (AIVs) have been detected from wild birds and domestic poultry worldwide. Serious diseases combined with secondary infection have caused high mortality and great economic losses to poultry industry. Therefore, simple, rapid, sensitive and accurate methods suitable for field detection of H9N2 AIVs are crucial to efficiently control virus infection and spread in time. In ...
DOI:
10.1111/tbed.13063

25. Dietary Intake of Nutrients Involved in One-Carbon Metabolism and Risk of Gastric Cancer: A Prospective Study

Author:
Pierre-Antoine Dugué; Julie K. Bassett; Maree T. Brinkman; Melissa C. Southey; Jihoon E. Joo; Ee Ming Wong; Roger L. Milne; Dallas R. English; Graham G. Giles; Alex Boussioutas; Hazel Mitchell; Allison M. Hodge
Source:
Nutrition and cancer 2019 v.71 no.4 pp. 605-614
ISSN:
1532-7914
Subject:
DNA repair; DNA replication; Helicobacter pylori; betaine; biotin; carcinogenesis; choline; confidence interval; folic acid; food frequency questionnaires; food intake; gene expression regulation; metabolism; methionine; niacin; nutrients; nutrition risk assessment; odds ratio; pantothenic acid; prospective studies; pyridoxine; regression analysis; riboflavin; stomach neoplasms; thiamin; vitamin B12
Abstract:
... Dietary intakes of B vitamins and other components involved in one-carbon metabolism, which is necessary for DNA replication, DNA repair, and regulation of gene expression, may be associated with carcinogenesis. We investigated associations between intakes of 11 nutrients (thiamine, riboflavin, niacin, pantothenic acid, vitamin B6, biotin, folate, vitamin B12, methionine, choline, and betaine) and ...
DOI:
10.1080/01635581.2019.1577982

26. Dietary biotin requirement of fingerling Channa punctatus (Bloch)

Author:
Seemab Zehra; Mukhtar A. Khan
Source:
Journal of applied aquaculture 2019 v.31 no.3 pp. 236-253
ISSN:
1545-0805
Subject:
Channa punctata; biotin; casein; crude protein; energy; feed conversion; fingerlings; liver; protein deposition; pyridoxine; satiety; specific growth rate; weight gain
Abstract:
... Dietary biotin requirement of fingerling Channa punctatus (4.52 ± 0.46 g) was estimated by conducting a 16-week growth trial. Fish were fed casein gelatin-based purified diets (450 g/kg crude protein, 18.39 kJ/g gross energy) with seven levels of dietary biotin (0, 0.04, 0.09, 0.47, 1.02, 1.43, and 1.96 mg/kg diet) to triplicate groups near to satiation. Significantly higher absolute weight gain ( ...
DOI:
10.1080/10454438.2018.1545722

27. Different versions of the German nutrient database affect energy and nutrient intake among adolescents

Author:
Marjolein Haftenberger; Franziska Lehmann; Anna-Kristin Brettschneider; Almut Richter; Clarissa Lage Barbosa; Gert B.M. Mensink
Source:
Subtropical plant science 2019 v.82 pp. 103251
ISSN:
0485-2044
Subject:
adolescents; biotin; calcium; children; cholecalciferol; computers; copper; diet history; dietary surveys; energy intake; food composition; interviews; iron; magnesium; manganese; normal values; nutrient databanks; nutrient intake; nutrients
Abstract:
... Updates of food composition data complicate comparisons of energy and nutrient intake between consecutive dietary surveys. The effect of an update of the German Nutrient Database (BLS) from version BLS2.3 to BLS3.02 on calculated energy and nutrient intake was evaluated among 1272 adolescents aged 12 to 17 years from the first German children and adolescents nutrition survey EsKiMo. Food consumpti ...
DOI:
10.1016/j.jfca.2019.103251

28. Displacement Affinity Release of Antibodies from Injectable Hydrogels

Author:
Vincent Huynh; Ryan Gavin Wylie
Source:
ACS applied materials & interfaces 2019 v.11 no.34 pp. 30648-30660
ISSN:
1944-8252
Subject:
antibodies; binding capacity; binding sites; bioactive properties; biotin; cytokines; encapsulation; hydrogels; immunoglobulin G; interferometry; ligands; neoplasm cells; neoplasms; pellets; polycarboxybetaines; surface plasmon resonance; therapeutics
Abstract:
... Current methods to tune release rates of therapeutic antibodies (Abs) for local delivery are complex and routinely require bioconjugations that may reduce Ab bioactivity. To rapidly tune release profiles of bioactive Abs, we developed a biophysical interaction system within a neutravidin modified poly(carboxybetaine) hydrogel (pCB-NT) that tunes release rates of desthiobiotinylated Abs (D-Abs) usi ...
DOI:
10.1021/acsami.9b12572

29. Dissecting the Role of the Gut Microbiota and Diet on Visceral Fat Mass Accumulation (OR31-07-19)

Author:
Caroline Le Roy; Ruth Bowyer; Claire Steves; Tim Spector; Bell Jordana
Source:
Current developments in nutrition 2019 v.3 no.Supplement_1 pp. -
ISSN:
2475-2991
Subject:
adiposity; algorithms; biotin; dual-energy X-ray absorptiometry; females; food frequency questionnaires; food intake; intestinal microorganisms; magnesium; metabolic diseases; nutrient intake; nutrients; prediction; risk factors; sequence analysis; trans fatty acids; twins; visceral fat; vitamin E
Abstract:
... Accumulation of visceral fat mass (VFM) is a major risk factor for cardiovascular and metabolic disease. Both gut microbiota and diet have been shown to impact host adiposity in an interdependent manner, but the exact nature of their joint contributions has not been characterised. Here, we aimed to estimate and separate the effect of gut microbiota composition from that of nutrient intake on host ...
DOI:
10.1093/cdn/nzz037.OR31-07-19

30. Drug-Initiated Synthesis of Heterotelechelic Polymer Prodrug Nanoparticles for in Vivo Imaging and Cancer Cell Targeting

Author:
Daniele Vinciguerra; Anna Degrassi; Laura Mancini; Simona Mura; Julie Mougin; Patrick Couvreur; Julien Nicolas
Source:
Biomacromolecules 2019 v.20 no.7 pp. 2464-2476
ISSN:
1526-4602
Subject:
antineoplastic activity; antineoplastic agents; biotin; body weight changes; cytotoxicity; dyes; humans; hydrophilicity; image analysis; liver; lung neoplasms; mice; nanoparticles; near-infrared spectroscopy; neoplasm cells; particle size distribution; polymers
Abstract:
... “Drug-initiated” nitroxide-mediated synthesis of two well-defined, heterotelechelic polymer prodrugs (Mₙ = 1960–5260 g·mol–¹, Đ = 1.31–1.37) was performed by using the newly developed nitroxide exchange reaction. These polymers comprised, at the chain end, gemcitabine (Gem) as anticancer drug and either cyanine 7.5 (Cy7.5) as a near-infrared (NIR) dye suitable for in vivo imaging or biotin (Biot) ...
DOI:
10.1021/acs.biomac.9b00148

31. Dual-Reactivity trans-Cyclooctenol Probes for Sulfenylation in Live Cells Enable Temporal Control via Bioorthogonal Quenching

Author:
Samuel L. Scinto; Oshini Ekanayake; Uthpala Seneviratne; Jessica E. Pigga; Samantha J. Boyd; Michael T. Taylor; Jun Liu; Christopher W. am Ende; Sharon Rozovsky; Joseph M. Fox
Source:
Journal of the American Chemical Society 2019 v.141 no.28 pp. 10932-10937
ISSN:
1520-5126
Subject:
Lewis acids; biotin; models; peptides; post-translational modification; proteins; proteomics; reactive oxygen species; signal transduction; sulfenic acids
Abstract:
... Sulfenylation (RSH → RSOH) is a post-translational protein modification associated with cellular mechanisms for signal transduction and the regulation of reactive oxygen species. Protein sulfenic acids are challenging to identify and study due to their electrophilic and transient nature. Described here are sulfenic acid modifying trans-cycloocten-5-ol (SAM-TCO) probes for labeling sulfenic acid fu ...
DOI:
10.1021/jacs.9b01164
PubMed:
31246462
PubMed Central:
PMC6756850

32. The Effect of a Combination of an Arginine Silicate Complex and Magnesium Biotinate on Hair and Nail Growth in Rats (P06-026-19)

Author:
James Komorowski; Sara Perez Ojalvo; Sarah Sylla; Demet Cicek; Betul Demir; Cemal Orhan; Mehmet Tuzcu; Nurhan Sahin; Kazim Sahin
Source:
Current developments in nutrition 2019 v.3 no.Supplement_1 pp. -
ISSN:
2475-2991
Subject:
alopecia; arginine; bioavailability; biotin; blood; blood sampling; brittleness; diet; egg albumen; females; hair follicles; laboratory animals; magnesium; minerals; nutrients; rats; silicates; silicon; vascular endothelial growth factors
Abstract:
... Aging, as well as inadequate intake of vitamins and minerals, can negatively affect the growth, strength, and appearance of hair and nails. For instance, biotin deficiency leads to brittle nails and hair loss. Moreover, certain nutrients, such as arginine and silicon, promote the biological conditions needed for optimal hair and nail growth. JDS-ASB is a novel combination of inositol-stabilized ar ...
DOI:
10.1093/cdn/nzz031.P06-026-19

33. Effect of biotin supplementation on milk yield of Girolando cows reared in a tropical climate

Author:
P. J. B. Queiroz; D. C. Silva; P. A. C. Borges; A. C. B. R. Pedroso; A. P. A. Vinhal; R. E. Rabelo; L. A. F. Silva
Source:
Tropical animal health and production 2019 v.51 no.6 pp. 1661-1665
ISSN:
0049-4747
Subject:
Girolando; biotin; cows; dairy farming; feed supplements; lactation; milk yield; rearing; reproductive efficiency; tropics; vitamin supplements; Brazil
Abstract:
... The objective of this study was to evaluate the effect of biotin supplementation on milk yield and the reproductive efficiency in Girolando cows. The study was conducted on a dairy farm located in central Brazil, between April 2012 and December 2016. Thirty-six Girolando cows in their first lactation were used. The cows were distributed in two treatment groups, each with equivalent weight distribu ...
DOI:
10.1007/s11250-019-01862-w

34. Effect of maternal biotin, folic acid, and vitamin B12 supplementation before parturition on colostral and Holstein calf plasma concentrations in those vitamins

Author:
Mélissa Duplessis; Christiane L. Girard
Source:
Animal feed science and technology 2019 pp. -
ISSN:
0377-8401
Subject:
Holstein; biotin; blood; calves; calving; colostrum; confidence interval; cows; feed supplements; folic acid; immunoglobulin G; maternal nutrition; milking; neonates; prepartum period; progeny; rumen; vitamin B12; vitamin supplements
Abstract:
... The newborn calf relies on placental and colostral transfers of B vitamins to cover its requirements. This study was conducted to evaluate biotin, folate and vitamin B12 concentrations in colostrum of cows receiving those vitamin supplements and in plasma of their calves fed with their colostrum. According to a 2 × 2 factorial arrangement, non-lactating pregnant cows (n = 34) were assigned to 2 le ...
DOI:
10.1016/j.anifeedsci.2019.114241

35. Effect of nutrients on the growth of a new alpine strain of Haematococcus (Chlorophyceae) from New Zealand

Author:
Nilanjana Mazumdar; Phil M. Novis; Gabriel Visnovsky; Peter A. Gostomski
Source:
Phycological research 2019 v.67 no.1 pp. 21-27
ISSN:
1322-0829
Subject:
Haematococcus; aquaculture; astaxanthin; biomass production; biotin; carbon; cobalt; copper; culture flasks; industry; iron; manganese; microalgae; molybdenum; nitrogen; nutrients; sodium nitrate; thiamin; vitamin B12; zinc; New Zealand
Abstract:
... The microalga Haematococcus lacustris is a source of astaxanthin used widely in aquaculture, pharmaceutical, and cosmetic industries. A new strain of Haematococcus (LCR‐26C‐1f) isolated from the New Zealand alpine zone was evaluated in this study. The influence of vitamins, micronutrients, various carbon and nitrogen sources were investigated to maximize biomass production in batch cultures using ...
DOI:
10.1111/pre.12344

36. Electrochemical aptasensor for sulfadimethoxine detection based on the triggered cleavage activity of nuclease P1 by aptamer-target complex

Author:
Zuhai Bai; Yan Chen; Fei Li; Yunlei Zhou; Huanshun Yin; Shiyun Ai
Source:
Talanta 2019 v.204 pp. 409-414
ISSN:
0039-9140
Subject:
DNA; antibodies; aptasensors; biotin; detection limit; electrochemistry; electrodes; electrostatic interactions; enzyme activity; enzymes; gold; milk; nucleic acid hybridization; oligonucleotides; sulfadimethoxine; veterinary drugs
Abstract:
... Herein, a simple and selective electrochemical method was developed for sulfadimethoxine detection based on the triggered cleavage activity of nuclease P1 by the formation of aptamer and sulfadimethoxine conjugate. After probe DNA was immobilized on gold electrode surface, aptamer DNA labeled with biotin at its 5′-terminal was then captured on electrode surface through the hybridization reaction b ...
DOI:
10.1016/j.talanta.2019.06.035

37. Engineering Corynebacterium glutamicum triggers glutamic acid accumulation in biotin-rich corn stover hydrolysate

Author:
Jingbai Wen; Jie Bao
Source:
Biotechnology for biofuels 2019 v.12 no.1 pp. 86
ISSN:
1754-6834
Subject:
Corynebacterium glutamicum; biomass; biotin; corn stover; feedstocks; fermentation; glucose; glutamic acid; hydrolysates; lignocellulose; metabolic engineering; oxoglutarate dehydrogenase (succinyl-transferring); penicillins; secretion
Abstract:
... BACKGROUND: Lignocellulose biomass contains high amount of biotin and resulted in an excessive biotin condition for cellulosic glutamic acid accumulation by Corynebacterium glutamicum. Penicillin or ethambutol triggers cellulosic glutamic acid accumulation, but they are not suitable for practical use due to the fermentation instability and environmental concerns. Efficient glutamic acid production ...
DOI:
10.1186/s13068-019-1428-5
PubMed:
31011369
PubMed Central:
PMC6463653

38. Engineering of vitamin prototrophy in Clostridium ljungdahlii and Clostridium autoethanogenum

Author:
Florence J. Annan; Bakir Al-Sinawi; Christopher M. Humphreys; Rupert Norman; Klaus Winzer; Michael Köpke; Sean D. Simpson; Nigel P. Minton; Anne M. Henstra
Source:
Applied microbiology and biotechnology 2019 v.103 no.11 pp. 4633-4648
ISSN:
0175-7598
Subject:
Clostridium acetobutylicum; Clostridium autoethanogenum; Clostridium ljungdahlii; Desulfotomaculum nigrificans; acetogens; auxotrophs; beta-alanine; biochemical pathways; biosynthesis; biotin; carbon; carbon monoxide; chromosomes; engineering; fastidious microorganisms; multigene family; nucleotide sequences; nutrient requirements; operon; pantolactone; pantothenic acid; plasmids; recombinant DNA; thiamin; yeast extract
Abstract:
... Clostridium autoethanogenum and Clostridium ljungdahlii are physiologically and genetically very similar strict anaerobic acetogens capable of growth on carbon monoxide as sole carbon source. While exact nutritional requirements have not been reported, we observed that for growth, the addition of vitamins to media already containing yeast extract was required, an indication that these are fastidio ...
DOI:
10.1007/s00253-019-09763-6
PubMed:
30972463
PubMed Central:
PMC6505512

39. Entropy/Enthalpy Compensation in Anion Binding: Biotin[6]uril and Biotin-l-sulfoxide[6]uril Reveal Strong Solvent Dependency

Author:
Nicolaj N. Andersen; Kristina Eriksen; Micke Lisbjerg; Mille E. Ottesen; Birgitte O. Milhøj; Stephan P. A. Sauer; Michael Pittelkow
Source:
Journal of organic chemistry 2019 v.84 no.5 pp. 2577-2584
ISSN:
1520-6904
Subject:
acetonitrile; anions; binding properties; biotin; calorimetry; chemical reactions; chemical structure; computational methodology; diastereoselectivity; enthalpy; entropy; macrocyclic compounds; nuclear magnetic resonance spectroscopy; organic chemistry; pH; receptors; solvents; synthesis; titration
Abstract:
... Binding of anions using macrocyclic structures with a nonpolar interior using the CH···anion interaction as the recognition motif has gained popularity in the past few years, and such receptors often rely on a subtle interplay between enthalpic and entropic factors. For these types of receptors solvation of both the anion and the binding pocket of the macrocyclic host play important roles in the o ...
DOI:
10.1021/acs.joc.8b02797

40. Erroneous thyroid diagnosis due to over-the-counter biotin

Author:
Stephanie Charles; Nidhi Agrawal; Manfred Blum
Source:
Nutrition 2019 v.57 pp. 257-258
ISSN:
0899-9007
Subject:
biotin; blood serum; chemical species; dietary supplements; immunoassays; patients; physicians; streptavidin; thyroid diseases; thyroid function tests; thyrotropin; thyroxine
Abstract:
... Objectives: Biotin is a component of the vitamin B complex used in standard immunoassays to detect serum levels of various hormones and non-hormones, including thyrotropin (TSH) and thyroxine. These assays involve a strong bond between streptavidin and biotin, which serves as an anchor for measured analytes. Large doses of exogenous biotin for the treatment of certain medical conditions have resul ...
DOI:
10.1016/j.nut.2018.05.005

41. Exploring a Tumor-Intrinsic PD-L1 Signal with Proximity-Dependent Biotin Identification in Lung Cancer Cells

Author:
Tiepeng Li; Fang Zhang; Peng Qin; Yao Wang; Axiang Wang; Lingdi Zhao; Benling Xu; Quanli Gao
Source:
Biochemistry 2019 v.58 no.18 pp. 2293-2296
ISSN:
1520-4995
Subject:
adenocarcinoma; antibodies; biotin; carcinogenesis; cell lines; gene overexpression; ligases; lung neoplasms; lungs; mass spectrometry; mutants; neoplasm cells; patients; signal transduction; streptavidin; therapeutics
Abstract:
... Blocking the PD-L1/PD-1 interaction with an antibody produces a durable response in patients with diverse advanced cancers. However, it remains elusive on whether the engagement of PD-L1 to PD-1 leads to tumor-intrinsic signaling. In this study, we aim to explore novel protein substrates participating in transducing this tumor-intrinsic PD-L1 signaling. To this end, we performed a BioID (proximity ...
DOI:
10.1021/acs.biochem.9b00113

42. Expression of microRNA‐like RNA‐2 (Fgmil‐2) and bioH1 from a single transcript in Fusarium graminearum are inversely correlated to regulate biotin synthesis during vegetative growth and host infection

Author:
Mao‐Wei Guo; Peng Yang; Jing‐Bo Zhang; Gang Liu; Qing‐Song Yuan; Wei‐Jie He; Jun‐Na Nian; Shu‐Yuan Yi; Tao Huang; Yu‐Cai Liao
Source:
Molecular plant pathology 2019 v.20 no.11 pp. 1574-1581
ISSN:
1464-6722
Subject:
Fusarium graminearum; Triticum; biogenesis; biosynthesis; biotin; complementary DNA; conidia; enzymes; fungi; gene expression regulation; genes; messenger RNA; mycelium; mycotoxins; rapid amplification of cDNA ends; sequence analysis; vegetative growth; virulence; wheat
Abstract:
... MicroRNA‐like RNAs (milRNAs) post‐transcriptionally down‐regulate target genes. We investigated Fusarium graminearum (Fg) milRNA expression during fungal vegetative growth and infection of wheat. Small RNA sequencing identified 36 milRNAs from Fg, one of which, Fgmil‐2, had >100 transcripts per million in conidia, mycelia and infected wheat, with the highest expression in conidia and the lowest ex ...
DOI:
10.1111/mpp.12859
PubMed:
31385410
PubMed Central:
PMC6804420

43. FabG: from a core to circumstantial catalyst

Author:
Anirudh P. Shanbhag
Source:
Biotechnology letters 2019 v.41 no.6-7 pp. 675-688
ISSN:
0141-5492
Subject:
active sites; acyl carrier protein; alcohols; alkanes; antibiotics; biochemical pathways; biotin; catalysts; enzymes; eukaryotic cells; fatty acids; gene duplication; gene pool; industry; lipoic acid; prokaryotic cells; protein-protein interactions; secondary metabolites
Abstract:
... Core biochemical pathways such as Fatty-acid synthesis II (FAS II) is ascribed to the synthesis of fatty-acids, biotin and lipoic acid in prokaryotes. It has two dehydrogenases namely, FabG and FabI which interact with the fatty-acid chain bound to Acyl-carrier protein (ACP), a well-studied enzyme which binds to substrates of varying lengths. This protein–protein interaction ‘broadens’ the active ...
DOI:
10.1007/s10529-019-02678-2

44. Fabrication of Furan-Functionalized Quinazoline Hybrids: Their Antibacterial Evaluation, Quantitative Proteomics, and Induced Phytopathogen Morphological Variation Studies

Author:
Qing-Su Long; Li-Wei Liu; Yong-Liang Zhao; Pei-Yi Wang; Biao Chen; Zhong Li; Song Yang
Source:
Journal of agricultural and food chemistry 2019 v.67 no.40 pp. 11005-11017
ISSN:
1520-5118
Subject:
Xanthomonas axonopodis pv. citri; Xanthomonas oryzae pv. oryzae; agrochemicals; antibacterial properties; antibiotics; bacteria; bacterial diseases of plants; bacterial infections; bioassays; biochemical pathways; biotin; blight; fluorescence emission spectroscopy; glucose; in vivo studies; ingredients; median effective concentration; monitoring; plant pathogens; protein synthesis; proteomics; scanning electron microscopy
Abstract:
... The limited number of agrochemicals targeting plant bacterial diseases has driven us to develop highly efficient, low-cost, and versatile antibacterial alternatives. Herein, a novel type of simple furan-functionalized quinazolin-4-amines was systematically fabricated and screened for their antibacterial activity. Bioassay results revealed that compounds C₁ and E₄ could substantially block the grow ...
DOI:
10.1021/acs.jafc.9b03419

45. Formulation and manufacturing of lymphatic targeting liposomes using microfluidics

Author:
Swapnil Khadke; Carla B. Roces; Allan Cameron; Andrew Devitt; Yvonne Perrie
Source:
Journal of controlled release 2019 v.307 pp. 211-220
ISSN:
0168-3659
Subject:
antigens; avidin; biotin; injection site; intramuscular injection; lymph nodes; macrophages; manufacturing; microfluidic technology; neoplasms; phosphatidylserines; screening; vaccination
Abstract:
... The lymphatics are a target for a range of therapeutic purposes, including cancer therapy and vaccination, and both vesicle size and charge have been considered as factors controlling lymphatic targeting. Within this work, a range of liposomal formulations were investigated to develop a liposomal lymphatic targeting system. Initial screening of formulations considered the effect of charge, with ne ...
DOI:
10.1016/j.jconrel.2019.06.002

46. The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2

Author:
Joanna R. Kovalski; Aparna Bhaduri; Ashley M. Zehnder; Poornima H. Neela; Yonglu Che; Glenn G. Wozniak; Paul A. Khavari
Source:
Molecular cell 2019 v.73 no.4 pp. 830-844.e12
ISSN:
1097-2765
Subject:
biotin; cell cycle; enzyme activity; gene editing; humans; mutants; neoplasms; oncogenes; phosphatidylinositol 3-kinase; plasma membrane; proteins; proteome; proteomics; screening; transcription (genetics)
Abstract:
... Proximity-dependent biotin labeling (BioID) may identify new targets for cancers driven by difficult-to-drug oncogenes such as Ras. Therefore, BioID was used with wild-type (WT) and oncogenic mutant (MT) H-, K-, and N-Ras, identifying known interactors, including Raf and PI3K, as well as a common set of 130 novel proteins proximal to all Ras isoforms. A CRISPR screen of these proteins for Ras depe ...
DOI:
10.1016/j.molcel.2018.12.001
PubMed:
30639242
PubMed Central:
PMC6386588

47. Functional Replacement of the BioC and BioH Proteins of Escherichia coli Biotin Precursor Biosynthesis by Ehrlichia chaffeensis Novel Proteins

Author:
Xudong Hang; Qi Zeng; Liping Zeng; Jia Jia; Hongkai Bi
Source:
Current microbiology 2019 v.76 no.5 pp. 626-636
ISSN:
0343-8651
Subject:
Anaplasma; Ehrlichia chaffeensis; Escherichia coli; biosynthesis; biotin; moieties; pathogens; sequence diversity
Abstract:
... The biosynthesis of the pimelate moiety of biotin in Escherichia coli requires two specialized proteins, BioC and BioH. However, the enzymes that have BioC- or BioH-like activities show remarkable sequence diversity among biotin-producing bacteria. Here, we report that the intracellular rickettsial pathogen Ehrlichia chaffeensis encodes two novel proteins, BioT and BioU, which functionally replace ...
DOI:
10.1007/s00284-019-01669-w

48. Growth, Polymorphism, and Spatially Controlled Surface Immobilization of Biotinylated Variants of IAPP₂₁–₂₇ Fibrils

Author:
Torsten John; Juhaina Bandak; Nilushiya Sarveson; Claudia Hackl; Herre Jelger Risselada; Andrea Prager; Christian Elsner; Bernd Abel
Source:
Biomacromolecules 2019 v.21 no.2 pp. 783-792
ISSN:
1526-4602
Subject:
alanine; amyloid; biotin; coatings; fluorescence; fluorescence microscopy; isoleucine; leucine; molecular dynamics; noninsulin-dependent diabetes mellitus; phenylalanine; polypeptides; scanning electron microscopy; simulation models
Abstract:
... The islet amyloid polypeptide (IAPP) is a regulatory peptide that can aggregate into fibrillar structures associated with type 2 diabetes. In this study, the IAPP₂₁–₂₇ segment was modified with a biotin linker at the N-terminus (Btn-GNNFGAIL) to immobilize peptide fibrils on streptavidin-coated surfaces. Key residues for fibril formation of the N-terminal biotinylated IAPP₂₁–₂₇ segment were identi ...
DOI:
10.1021/acs.biomac.9b01466

49. A G‐quadruplex/hemin DNAzyme‐based microchip electrophoresis chemiluminescence assay for highly sensitive detection of biotin in flour

Author:
Jian Li; Shuting Li; Jingjin Zhao; Yong Huang; Shulin Zhao
Source:
Electrophoresis 2019 v.40 no.16-17 pp. 2157-2164
ISSN:
0173-0835
Subject:
biotin; catalytic activity; chemiluminescence; detection limit; electrophoresis; flour; hydrogen peroxide; microchip technology; quantitative analysis; redox reactions; streptavidin
Abstract:
... Quantitative analysis of biotin in biological fluids, foods, and pharmaceutical is important for diagnosis and treatment of biotin‐related diseases and health maintenance. In this work, a novel G‐quadruplex/hemin DNAzyme‐based microchip electrophoresis chemiluminescence (CL) assay method was established for rapid and highly sensitive detection of biotin. This method is based on the specific bindin ...
DOI:
10.1002/elps.201800535

50. Highly Homogeneous Biotinylated Carbon Nanodots: Red-Emitting Nanoheaters as Theranostic Agents toward Precision Cancer Medicine

Author:
Cinzia Scialabba; Alice Sciortino; Fabrizio Messina; Gianpiero Buscarino; Marco Cannas; Giuseppina Roscigno; Gerolama Condorelli; Gennara Cavallaro; Gaetano Giammona; Nicolò Mauro
Source:
ACS applied materials & interfaces 2019 v.11 no.22 pp. 19854-19866
ISSN:
1944-8252
Subject:
antineoplastic activity; antineoplastic agents; biotin; carbon; cell membranes; death; fever; fluorescence; heat; humans; image analysis; medicine; models; nanotechnology; near infrared radiation; neoplasm cells; neoplasms; patients; physicochemical properties; polymers; precision medicine; receptors
Abstract:
... Very recent red-emissive carbon nanodots (CDs) have shown potential as near-infrared converting tools to produce local heat useful in cancer theranostics. Besides, CDs seem very appealing for clinical applications combining hyperthermia, imaging, and drug delivery in a single platform capable of selectively targeting cancer cells. However, CDs still suffer from dramatic dot-to-dot variability issu ...
DOI:
10.1021/acsami.9b04925