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You searched for: Publication Year 2018 Remove constraint Publication Year: 2018 Subject term antigens Remove constraint Subject term: antigens
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1. A FRET immunosensor for sensitive detection of CA 15-3 tumor marker in human serum sample and breast cancer cells using antibody functionalized luminescent carbon-dots and AuNPs-dendrimer aptamer as donor-acceptor pair

Author:
Somayeh Mohammadi; Abdollah Salimi; Somayeh Hamd-Ghadareh; Fardin Fathi; Farzad Soleimani
Source:
Analytical biochemistry 2018 v.557 pp. 18-26
ISSN:
0003-2697
Subject:
antibodies; antigens; blood serum; breast neoplasms; carbon quantum dots; fluorescence; humans; immunoassays; immunosensors; models; nanogold; neoplasm cells; oligonucleotides; silver
Abstract:
... We proposed a FRET immunosensing for detection of CA15-3 tumor marker by highly biospecific interactions between CA 15-3 antigen and the corresponding antibody and aptamer. In this sandwich type immunoassay, CA15-3 antibody-functionalized carbon dots and AuNPs labeled PAMAM-Dendrimer/aptamer were used as donor/acceptor, respectively. When CA 15-3 Ag was added to homogenous immunoassay, the strong ...
DOI:
10.1016/j.ab.2018.06.008

2. A GICA strip for Campylobacter jejuni real-time monitoring at meat production site

Author:
Xiaoqi Zang; Ke Kong; Haiyan Tang; Yuanyue Tang; Hong Tang; Xinan Jiao; Jinlin Huang
Source:
Lebensmittel-Wissenschaft + Technologie 2018 v.98 pp. 500-505
ISSN:
0023-6438
Subject:
Campylobacter jejuni; antigens; bacteria; chicken meat; feces; food safety; gold; immunoaffinity chromatography; meat production; monitoring; pork
Abstract:
... Campylobacter jejuni (C. jejuni) contamination occurs vastly and inevitably at the meat production site, which makes the real-time monitoring of C. jejuni an increasingly key part for the food safety control. Aiming to establish a C. jejuni on-site detection method, a colloidal gold immunochromatographic assay (GICA) was developed with a visual lowest detection limit of 10² CFU/μL of C. jejuni. Al ...
DOI:
10.1016/j.lwt.2018.08.049

3. A Highly Sensitive Real-time Immuno-PCR Assay for Detecting Benzo[a]pyrene in Food Samples by Application of Biotin-Streptavidin System

Author:
Zhen Ma; Huisheng Zhuang
Source:
Food analytical methods 2018 v.11 no.3 pp. 862-872
ISSN:
1936-9751
Subject:
DNA; antigens; benzo(a)pyrene; cross reaction; detection limit; ecosystems; gas chromatography-mass spectrometry; human health; immunoassays; polyclonal antibodies; polycyclic aromatic hydrocarbons; spectrometers; streptavidin; vegetables
Abstract:
... Benzo[a]pyrene (BaP), a kind of polycyclic aromatic hydrocarbon, has great potential hazards to the ecosystem and human health. In this study, based on the preparation of BaP immunogen and coating antigen, a specific polyclonal antibody targeting BaP was generated, and a highly sensitive real-time immuno-PCR assay using the biotin-streptavidin system (BA-IPCR) was developed for detecting BaP in ve ...
DOI:
10.1007/s12161-017-1046-4

4. A Novel Human Papillomavirus 16 L1 Pentamer-Loaded Hybrid Particles Vaccine System: Influence of Size on Immune Responses

Author:
Chengcheng Jia; Tingyuan Yang; Yongjiang Liu; Ali Zhu; Fei Yin; Yajun Wang; Lan Xu; Yan Wang; Mei Yan; Qingman Cai; Xiaoxu Liang; Ruijun Ju; Jianping Chen; Lianyan Wang
Source:
ACS applied materials & interfaces 2018 v.10 no.42 pp. 35745-35759
ISSN:
1944-8252
Subject:
adjuvants; lipids; vaccination; chemokines; uterine cervical neoplasms; antibodies; dendritic cells; lymphocytes; lymph nodes; females; Escherichia coli; Human papillomavirus type 16; antigens; humans; developing countries; immune response; markets; mortality; salts; vaccines; particle size; aluminum; polymers; cost effectiveness
Abstract:
... Cervical cancer remains the second-most prevalent female malignancy around the world, leading to a great majority of cancer-related mortality that occurs mainly in developing countries. Developing an effective and low-cost vaccine against human papillomavirus (HPV) infection, especially in medically underfunded areas, is urgent. Compared with vaccines based on HPV L1 viruslike particles (VLPs) in ...
DOI:
10.1021/acsami.8b11556

5. A Rapid Method for Antigen-Specific Hybridoma Clone Isolation

Author:
Xiuqing Li; Hongfen Bian; Siming Yu; Wei Xiao; Jianying Shen; Caifeng Lan; Kenan Zhou; Caihong Huang; Lei Wang; Dan Du; Yuehe Lin; Yong Tang
Source:
Analytical chemistry 2018 v.90 no.3 pp. 2224-2229
ISSN:
1520-6882
Subject:
Porcine epidemic diarrhea virus; antigens; clones; enzyme-linked immunosorbent assay; fluorescence; hybridomas; malachite green; methylcellulose; monoclonal antibodies; rapid methods
Abstract:
... Using an enzyme-linked immunosorbent assay (ELISA) and limited dilution methods to screen and clone antigen-specific hybridoma cells is extremely time-consuming and labor-intensive. This work features a simple and rapid cell surface fluorescence immunosorbent assay (CSFIA), designed for the detection and isolation of antigen-specific hybridoma clones. In this assay, antigens are first anchored to ...
DOI:
10.1021/acs.analchem.7b04595

6. A Simple Colorimetric System for Detecting Target Antigens by a Three-Stage Signal Transformation–Amplification Strategy

Author:
Albert M. Liao; Weihua Pan; James C. Benson; Andrew D. Wong; Benjamin J. Rose; G. Thomas Caltagirone
Source:
Biochemistry 2018 v.57 no.34 pp. 5117-5126
ISSN:
1520-4995
Subject:
absorption; antigens; bronchodilators; caffeine; chemical species; color; colorimetry; diagnostic techniques; enzyme activity; guanine; instrumentation; oligonucleotides; peroxidase; ribozymes; single-stranded DNA; theophylline
Abstract:
... Inexpensive, straightforward, and rapid medical diagnostics are becoming increasingly important for disease identification in time- and resource-limited settings. Previous attempts to link oligonucleotide-based aptamers and hammerhead ribozymes to form ligand-induced ribozymes have been successful in identifying a variety of small molecule and protein targets. Isothermal exponential amplification ...
DOI:
10.1021/acs.biochem.8b00523

7. A Sortase A Programmable Phage Display Format for Improved Panning of Fab Antibody Libraries

Author:
Henry D. Wilson; Xiuling Li; Haiyong Peng; Christoph Rader
Source:
Journal of molecular biology 2018 v.430 no.21 pp. 4387-4400
ISSN:
0022-2836
Subject:
antibody engineering; antigens; bacteriophages; biotinylation; catalytic activity; drugs; humans; monoclonal antibodies; neoplasm cells; neoplasms; rabbits; sortase A
Abstract:
... Phage display of combinatorial antibody libraries is a versatile tool in the field of antibody engineering, with diverse applications including monoclonal antibody (mAb) discovery, affinity maturation, and humanization. To improve the selection efficiency of antibody libraries, we developed a new phagemid display system that addresses the complication of bald phage propagation. The phagemid facili ...
DOI:
10.1016/j.jmb.2018.09.003
PubMed:
30213726
PubMed Central:
PMC6186506

8. A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming Multicellular Morphologies and Patterns

Author:
David S. Glass; Ingmar H. Riedel-Kruse
Source:
Cell 2018 v.174 no.3 pp. 649-658.e16
ISSN:
0092-8674
Subject:
Escherichia coli; adhesins; antigens; biochemical pathways; biofilm; cell adhesion; models; separation; synthetic biology
Abstract:
... Synthetic multicellular systems hold promise as models for understanding natural development of biofilms and higher organisms and as tools for engineering complex multi-component metabolic pathways and materials. However, such efforts require tools to adhere cells into defined morphologies and patterns, and these tools are currently lacking. Here, we report a 100% genetically encoded synthetic pla ...
DOI:
10.1016/j.cell.2018.06.041

9. A Two-Step Immunocapture LC/MS/MS Assay for Plasma Stability and Payload Migration Assessment of Cysteine–Maleimide-Based Antibody Drug Conjugates

Author:
Linlin Dong; Chao Li; Charles Locuson; Susan Chen; Mark G. Qian
Source:
Analytical chemistry 2018 v.90 no.10 pp. 5989-5994
ISSN:
1520-6882
Subject:
albumins; antibodies; antigens; cost effectiveness; drugs; humans; magnetism; mice; monitoring; temperature
Abstract:
... Plasma stability assessment under physiological temperature is an essential step for developing and optimizing antibody drug conjugate (ADC) molecules, especially those with cleavable linkers. The assessment of plasma stability often requires monitoring multiple analytes using a combination of bioanalytical assays for free payloads, conjugated payloads (or conjugated antibodies), total antibodies, ...
DOI:
10.1021/acs.analchem.8b00694

10. A bead-based suspension array for the detection of Salmonella antibodies in pig sera

Author:
Fimme J. van der Wal; René P. Achterberg; Catharina B. M. Maassen
Source:
BMC veterinary research 2018 v.14 no.1 pp. 226
ISSN:
1746-6148
Subject:
Salmonella; agglutination; antibodies; antibody detection; antigens; bacteriology; blood serum; enzyme-linked immunosorbent assay; food animals; herds; lipopolysaccharides; pathogens; rabbits; serology; seroprevalence; serotypes; slaughter; swine; triazines; veterinary medicine
Abstract:
... BACKGROUND: Slaughter pigs are monitored for the presence of the zoonotic pathogen Salmonella, using both serology and bacteriology. ELISAs used to investigate pig herds are based on the detection of antibodies against components of the Salmonella cell envelope. Nearly all Salmonella isolates in food-producing animals are serovars of Salmonella enterica subspecies enterica, distributed over variou ...
DOI:
10.1186/s12917-018-1541-x
PubMed:
30053875
PubMed Central:
PMC6063008

11. A biobanking platform for fish-borne zoonotic parasites: a traceable system to preserve samples, data and money

Author:
Ángel F. González; Helena Rodríguez; Luis Outeiriño; Carlos Vello; Christian Larsson; Santiago Pascual
Source:
Fisheries research 2018 v.202 pp. 29-37
ISSN:
0165-7836
Subject:
DNA; Internet; antigens; clinical trials; computer software; databases; fish; hosts; infrastructure; monitoring; morphometry; parasites; proteins; stakeholders; traceability
Abstract:
... A biobank is a non-profit service that hosts a collection of biological samples organized as a technical unit with defined quality criteria, order and destination, to ensure full traceability of the sample and, above all, is a valuable underpinning for medical and biological research. We present the first biobank for marine biological samples, stemming mainly from the activity developed on zoonoti ...
DOI:
10.1016/j.fishres.2017.03.014

12. A bivalent vaccine derived from attenuated Salmonella expressing O-antigen polysaccharide provides protection against avian pathogenic Escherichia coli O1 and O2 infection

Author:
Yue Han; Qing Liu; Stephan Willias; Kang Liang; Pei Li; Anchun Cheng; Qingke Kong
Source:
Vaccine 2018 v.36 no.8 pp. 1038-1046
ISSN:
0264-410X
Subject:
Escherichia coli; O-antigens; Salmonella Typhimurium; Western blotting; antibacterial properties; antibodies; antigens; biosynthesis; blood serum; chickens; complement; financial economics; immune response; immunization; immunoglobulin G; mixing; models; multigene family; oxygen; plasmids; poultry industry; serotypes; vaccines; virulence
Abstract:
... Avian pathogenic Escherichia coli (APEC), a leading cause of avian airsacculitis and colibacillosis, is responsible for significant economic loss in the poultry industry. APEC serogroups O1, O2, and O78 are predominantly associated with disease. Lipopolysaccharide (LPS) O-antigen has been shown to be a potent antigen for inducing specific protective immune responses. Therefore, we sought to develo ...
DOI:
10.1016/j.vaccine.2018.01.036

13. A canine adenovirus type 2 vaccine vector confers protection against foot-and-mouth disease in guinea pigs

Author:
Annebel R. De Vleeschauwer; Xiaocui Zhou; David J. Lefebvre; Annabelle Garnier; Fleur Watier; Charly Pignon; Sandrine A. Lacour; Stephan Zientara; Labib Bakkali-Kassimi; Kris De Clercq; Bernard Klonjkowski
Source:
Vaccine 2018 v.36 no.16 pp. 2193-2198
ISSN:
0264-410X
Subject:
vector vaccines; antigens; humoral immunity; capsid; antibodies; hosts; models; disease control; vaccination; Canine mastadenovirus A; mice; structural proteins; foot-and-mouth disease; immune response; Foot-and-mouth disease virus; guinea pigs; emulsions
Abstract:
... Vaccination is a key element in the control of foot-and-mouth disease (FMD). The majority of the antigenic sites that induce protective immune responses are localized on the FMD virus (FMDV) capsid that is formed by four virus-encoded structural proteins, VP1 to VP4. In the present study, recombinant canine adenovirus type 2 (CAV2)-based FMD vaccines, Cav-P1/3C R° and Cav-VP1 R°, respectively expr ...
DOI:
10.1016/j.vaccine.2018.02.074

14. A colorimetric immunoassay based on glucose oxidase-induced AuNP aggregation for the detection of fumonisin B1

Author:
Xirui Chen; Yi Liang; Wenjing Zhang; Yuankui Leng; Yonghua Xiong
Source:
Talanta 2018 v.186 pp. 29-35
ISSN:
0039-9140
Subject:
antigens; color; colorimetry; corn; diagnostic techniques; enzyme-linked immunosorbent assay; food contamination; fumonisin B1; glucose; glucose oxidase; hydrogen peroxide; nanogold; oxidation; peroxidase; point-of-care systems; screening; ultra-performance liquid chromatography
Abstract:
... Naked-eye readout colorimetric signal-based assays are promising for high-throughput screening detection and point-of-care diagnostics in resource-constrained regions. Here, a novel direct competitive plasmonic enzyme-linked immunosorbent assay (dc-pELISA) based on gold nanoparticle (AuNP) aggregation with highly sensitive and robust naked-eye readout signal was developed and used to detect fumoni ...
DOI:
10.1016/j.talanta.2018.04.018

15. A competitive immunoassay for zearalenone with integrated poly(dimethysiloxane) based microarray assay

Author:
Li Li; Shuai Ren; Manyu Shao; Sarah De Saeger; Suquan Song; Liping Yan
Source:
Analytical methods 2018 v.10 no.33 pp. 4036-4043
ISSN:
1759-9679
Subject:
European Union; antigens; corn flour; cross reaction; detection limit; enzyme-linked immunosorbent assay; liquid chromatography; microarray technology; monoclonal antibodies; peroxidase; tandem mass spectrometry; zearalenone
Abstract:
... A monoclonal antibody (mAb) (2B10) against zearalenone (ZEN) was prepared for the first time in this study. The mAb was class specific and showed no cross reactivity to other groups of mycotoxins. A competitive microarray assay based on a novel solid supporting material, an integrated poly(dimethylsiloxane) (iPDMS), is proposed for qualitative and/or semiquantitative determination of ZEN in cereal ...
DOI:
10.1039/c8ay01307a

16. A comprehensive proteomics study on edible bird’s nest using new monoclonal antibody approach and application in quality control

Author:
Zack C.F. Wong; Gallant K.L. Chan; Long Wu; Henry H.N. Lam; Ping Yao; Tina T.X. Dong; Karl W.K. Tsim
Source:
Journal of food composition and analysis 2018 v.66 pp. 145-151
ISSN:
0889-1575
Subject:
Apodidae; antigens; chickens; chitinase; detection limit; enzyme-linked immunosorbent assay; mammals; monoclonal antibodies; mucins; proteomics; quality control; secretion
Abstract:
... Edible bird’s nest (EBN) is a glue-like substance deriving from salivary secretion by specific swiftlets, and protein is considered as the main component of EBN. Accounting over 50% by weight, the exact identities of EBN proteins are still not well understood, due to difficulties of extraction, purification and identification. By using EBN proteins as antigens, 31 monoclonal antibodies specificall ...
DOI:
10.1016/j.jfca.2017.12.014

17. A designer bow-tie combination therapeutic platform: An approach to resistant cancer treatment by simultaneous delivery of cytotoxic and anti-inflammatory agents and radiation

Author:
Rakesh K. Pathak; Uttara Basu; Anis Ahmad; Shrita Sarkar; Anil Kumar; Bapurao Surnar; Saba Ansari; Katarzyna Wilczek; Michael E. Ivan; Brian Marples; Nagesh Kolishetti; Shanta Dhar
Source:
Biomaterials 2018 v.187 pp. 117-129
ISSN:
0142-9612
Subject:
DNA damage; anti-inflammatory agents; antigens; cell respiration; cytotoxicity; drug therapy; fractionation; in vitro studies; inflammation; irradiation; metabolism; metastasis; mitochondria; models; neoplasm cells; patients; prostatic neoplasms; radiotherapy; stem cells
Abstract:
... Multimodal therapies are used to treat advanced cancers including castration-resistant prostate cancer to manage the biological characteristics of the tumors like inflammation, bone metastases, and participation of metabolically altered cancer stem cells (CSCs) that have integral roles in disease dissemination and progression. We developed a multifunctional polymer-based self-assembled technology ...
DOI:
10.1016/j.biomaterials.2018.08.062

18. A disposable immunosensor using ITO based electrode modified by a star-shaped polymer for analysis of tumor suppressor protein p53 in human serum

Author:
Muhammet Aydın; Elif Burcu Aydın; Mustafa Kemal Sezgintürk
Source:
Biosensors & bioelectronics 2018 v.107 pp. 1-9
ISSN:
0956-5663
Subject:
detection limit; blood serum; antigens; humans; biomarkers; antibodies; immunosensors; monitoring; atomic force microscopy; dielectric spectroscopy; electrodes; electrochemistry; epoxides; scanning electron microscopy; chemical bonding; Fourier transform infrared spectroscopy; polymers; colorectal neoplasms; tumor suppressor protein p53; Raman spectroscopy
Abstract:
... Label-free immunosensor based on tetra armed star-shaped poly(glycidylmethacrylate) (StarPGMA) modified disposable ITO electrode was developed for detection of p53 protein, an important colorectal cancer biomarker. This disposable biosensor was fabricated by spin-coating technique using star-shaped StarPGMA with epoxy side groups. After formation of a stable film with epoxy ends, anti-p53 antibodi ...
DOI:
10.1016/j.bios.2018.02.017

19. A dye-sensitized solar cell acting as the electrical reading box of an immunosensor: Application to CEA determination

Author:
Liliana A.A.N.A. Truta; Felismina T.C. Moreira; M. Goreti F. Sales
Source:
Biosensors & bioelectronics 2018 v.107 pp. 94-102
ISSN:
0956-5663
Subject:
antigens; biomarkers; dielectric spectroscopy; disease diagnosis; electrochemistry; electrodes; fluorine; glass; immunosensors; monitoring; neoplasms; oxides; photovoltaic cells; systems engineering; urine
Abstract:
... Monitoring cancer biomarkers in biological fluids has become a key tool for disease diagnosis, which should be of easy access anywhere in the world. The possibility of reducing basic requirements in the field of electrochemical biosensing may open doors in this direction. This work proposes for this purpose an innovative electrochemical immunosensing system using a photovoltaic cell as an electric ...
DOI:
10.1016/j.bios.2018.02.011

20. A glucuronoxylomannan-like glycan produced by Trichosporon mucoides

Author:
Ana Claudia G. Zimbres; Priscila C. Albuquerque; Luna S. Joffe; Taiane N. Souza; Leonardo Nimrichter; Stefânia O. Frazão; Patricia Albuquerque; Fernanda L. Fonseca; Marcio L. Rodrigues
Source:
Fungal genetics and biology 2018 v.121 pp. 46-55
ISSN:
1087-1845
Subject:
Cryptococcus neoformans; Galleria mellonella; Trichosporon asahii; antigens; functional diversity; glucuronic acid; knockout mutants; macrophages; mannose; models; monoclonal antibodies; pathogens; phagocytosis; polysaccharides; receptors; xylose; yeasts
Abstract:
... Trichosporon asahii shares with Cryptococcus species the ability to produce glucuronoxylomannan (GXM), an immunomodulatory fungal polysaccharide. The ability of other opportunistic species of Trichosporon to produce GXM-like polysaccharides is unknown. In this study, we observed that T. mucoides was less pathogenic than T. asahii in an infection model of Galleria mellonella and asked whether this ...
DOI:
10.1016/j.fgb.2018.09.009