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... 24-Epibrassinolide (EBL) is considered the most probable brassinosteroid (BR) candidate that could be used for practical application in agriculture. EBL-induced stress-protective properties were evaluated in in vitro-grown tomato (Solanum lycopersicum L.) varieties Pusa Ruby (susceptible to nematodes) and PNR-7 (resistant to nematodes) during nematode pathogenesis. Sterilized tomato seeds treated ...
... To explore the RNA-dependent RNA polymerase (RdRP) function of the 3D protein of duck hepatitis A virus type 1 (DHAV-1), the gene was cloned into the pET-32a(+) vector for prokaryotic expression. The 3′ untranslated region (3′ UTR) of DHAV-1 together with a T7 promoter was cloned into the pMD19-T vector for in vitro transcription of 3′ UTR RNA, which was further used as a template in RNA-dependent ...
carbonates; chemical reactions; ethylene; ligands; sulfates
Abstract:
... Novel multimetallic copper pyrazolate complexes, inaccessible using simple pyrazole ligands due to competing, alternative structural motifs, can be obtained by locking pairs of pyrazole ligands together with ethylene tethers. Nanojars based on this tethered pyrazole ligand display unexpected total selectivity for the carbonate over the sulfate ion. ...
... Changes in the activity of Na⁺/K⁺-ATPase and content of membrane lipids (phospholipids) in the gills and hepatopancreas of the blue mussel Mytilus edulis L. have been studied during a sharp temperature increase under aquarian managed conditions. The most pronounced changes were recorded in mollusk gills. A correlation of changes in the activity of membrane-bound Na⁺/K⁺-ATPase and phospholipid cont ...
Dianthus chinensis; antioxidant activity; enzyme activity; ethylene; guard cells; hydrogen peroxide; hyperhydricity; in vitro culture; in vitro studies; micropropagation; plantlets; silver nitrate; water content
Abstract:
... Hyperhydricity symptoms are common and significant during the in vitro culture of Dianthus chinensis L. and greatly affect the micropropagation and regeneration of cultured plantlets. However, effective measures for preventing such abnormalities have not been developed for this species. Silver nitrate (AgNO₃) has been shown to revert hyperhydric plantlets to a normal state. Nevertheless, the effec ...
Cephalotaxus; Fourier transform infrared spectroscopy; alkaloids; electrospray ionization mass spectrometry; isomers; nuclear magnetic resonance spectroscopy; Vietnam
Abstract:
... Eight alkaloidal constituents have been isolated and structurally elucidated from Cephalotaxus mannii Hook. f. collected from the highland Lam Dong Province, Vietnam. They are: cephalotaxine (1), isoharringtonine (2), norisoharringtonine (3), desoxyharringtonine (4), nordesoxyharringtonine (5), cephalotaxine 𝛽 -N-oxide (6), 3-epi-schellhammericine (7), and an isomer of 3-epi-schellhammericine (8) ...
borates; chemical reactions; nuclear magnetic resonance spectroscopy
Abstract:
... Allyl borates, a new class of monofunctional polyhomologation initiators, are reported. These monofunctional initiators are less sensitive and more effective towards polymethylene-based architectures. As an example, the synthesis of α-vinyl-ω-hydroxypolymethylenes is given. By designing/synthesizing different allylic borate initiators, and using ¹H and ¹¹B NMR spectroscopy, the initiation mechanis ...
Rafael R. Cantú; Celso Aita; Alexandre Doneda; Diego A. Giacomini; Alexandre Dessbesell; Marlon Arenhardt; Géssica G. De Bastiani; Stefen B. Pujol; Philippe Rochette; Martin H. Chantigny; Sandro J. Giacomini
... Alternative fertilization practices are needed for reducing gaseous and leaching N losses at high urea application rates. The objective of this study was to compare gaseous N emissions (N₂O and NH₃) and NO₃ ⁻ concentrations in the soil solution during two successive lettuce cropping seasons under contrasting fertilization practices. Treatments were fertilization with regular urea (U), urea treated ...
V. G. Pshennikova; N. A. Barashkov; A. V. Solovyev; G. P. Romanov; E. E. Diakonov; N. N. Sazonov; I. V. Morozov; A. A. Bondar; O. L. Posukh; L. U. Dzhemileva; E. K. Khusnutdinova; M. I. Tomsky; S. A. Fedorova
... Тhe DNA testing of autosomal recessive deafness type 1A (DFNB1A, MIM 220290) is complicated when deaf patients have only monoallelic (heterozygous) recessive mutations in the GJB2 (Сх26) gene that is uninformative for establishment of diagnosis. Such patients may be “random” heterozygous carriers of GJB2 mutations as well as have the mutant allele in a cis-regulatory region of GJB2 gene, in elemen ...
... Biliary atresia (BA) is a rare and serious liver disease in newborn infants. Previously, we reported that non-parenchymal cell (NPC) fractions from cirrhotic liver of BA may contain hepatic stem/progenitor cells in primary culture of NPC fractions. In this study, NPC fractions were subjected to primary or passage culture and found that clusters of hepatocyte-like cells appear even without adding h ...
... Recent articles have provided estimates of evaporative flux from water tables in deserts that span 5 orders of magnitude. In this paper, we present an analytical calculation that indicates aquifer vapor flux to be limited to 0.01 mm/yr for sites where there is negligible recharge and the water table is well over 20 m below the surface. This value arises from the geothermal gradient, and therefore, ...
... The emergence and disappearance of antigenic variants of foot-and-mouth disease virus (FMDV) during a field outbreak occurs periodically due to the volatile nature of its genome. In the present analysis, change in antigenic behavior of serotype O FMDV during the serial cytolytic passage in the absence of immune pressure was observed. Initially, the isolate showed a poor antigenic match (relationsh ...
... The ability of alkalophilic micromycetes of the Emericellopsis alkalinа to produce antimicrobial peptides has been studied. Evaluation of the spectrum and the yield of antibiotic compounds has allowed us to choose a promising producer of peptide antimycotics, Emericellopsis аlkalinа А118. The producer exhibits antifungal activity against conditionally pathogenic yeast and mold fungi, i.e., Candida ...
DNA damage; antioxidant activity; apigenin; apoptosis; cytotoxicity; diabetes; enzyme activity; enzymes; free radical scavengers; in vitro studies; islets of Langerhans; lipid peroxidation; oxidative stress; protective effect; protein synthesis; rats; reactive oxygen species; streptozotocin; therapeutics; transcription (genetics)
Abstract:
... Pancreatic beta cells are very sensitive to oxidative stress, which is one of the major causes of cell damages in diabetes. Growing interest has focused on the development of effective therapeutics to protect pancreatic cells from oxidative stress and searching for potentially protective antioxidants for treating diabetes. Apigenin, a plant-derived flavonoid, was investigated to determine whether ...
... On behalf of the journal, the American Geophysical Union, and the scientific community, the editors would like to sincerely thank those who reviewed manuscripts for Water Resources Research in 2016. Their time spent reading and commenting on manuscripts not only improves the manuscripts themselves but also increases the scientific rigor of future research in the field. Many of those listed below w ...
... Somaclonal variation refers to the genetic and epigenetic changes in plants regenerated from plant tissue culture. In this study, using intersimple sequence repeat (ISSR) molecular markers, the somaclonal variation during micropropagation of sugarcane using temporary immersion bioreactors (TIBs) was evaluated. Apices of the cultivar Mex 69-290 were established and multiplied by ten subcultures in ...
... The association of polymorphism T-786C of the endothelial NO-synthase gene NOS3 with the functional state of the left ventricle (LV) was studied among residents of the West Siberian region with ischemic heart disease (IHD), including that combined with diabetes mellitus type 2 (DMT2). Carriers of genotype–786TT had the greatest ejection fraction of the left ventricle in the group of patients witho ...
Burkholderiaceae; Micrococcaceae; Streptomycetaceae; Xanthomonadaceae; application rate; bacterial communities; clay; community structure; crop yield; equations; granite; manure spreading; mineral fertilizers; phosphorus; sandstone; soil bacteria; soil pH; soil productivity; China
Abstract:
... A plot experiment was conducted to understand the response of the soil bacterial community to manure application rates and the relationship between the composition of bacterial community and soil chemical properties. The experiment involved gradients of manure combined with chemical fertilizer in red soils from granite, red sandstone and red clay between 2013 and 2015. The soil bacterial community ...
... River networks that drain mountain landscapes alternate between narrow and wide valley segments. Within the wide segments, beaver activity can facilitate the development and maintenance of complex, multithread planform. Because the narrow segments have limited ability to retain water, carbon, and nutrients, the wide, multithread segments are likely important locations of retention. We evaluated hy ...
Daniela Gabriel; Stefano G.A. Draisma; William E. Schmidt; Tom Schils; Thomas Sauvage; Clio Maridakis; C. Frederico D. Gurgel; D. James Harris; Suzanne Fredericq
Gibsmithia; algae; genetic markers; new species; phylogeny; Egypt; Federated States of Micronesia; Hawaii; Indonesia; Philippines; Red Sea; Saudi Arabia
Abstract:
... The tropical alga previously recognized as Gibsmithia hawaiiensis (Dumontiaceae, Rhodophyta) was recently suggested to represent a complex of species distributed throughout the Indo‐Pacific Ocean and characterized by a peculiar combination of hairy (pilose) gelatinous lobes growing on cartilaginous stalks. Phylogenetic reconstructions based on three genetic markers are presented here with the incl ...
antioxidant activity; bioactive compounds; chemical reactions; coordination compounds; films (materials); food industry; luteolin; myricetin; quercetin; water solubility
Abstract:
... We assembled dietary, bioactive flavonoids into a metal coordinated network to form thin, surface-bound films and hollow capsules, overcoming the poor water solubility of free flavonoids. Films formed from quercetin, myricetin, luteolin and fisetin show radical scavenging activity, a renowned feature of their parent flavonoids, and can be reused over multiple cycles. These films are expected to ha ...
Fourier transform infrared spectroscopy; Marsilea quadrifolia; X-ray diffraction; adipocytes; cytotoxicity; dose response; energy-dispersive X-ray analysis; glucose; in vitro studies; insulin; metformin; nanogold; scanning electron microscopy; screening; transmission electron microscopy; viability
Abstract:
... This study aims mainly to provide an insight and understanding of the effect of glucose utilization efficiency of biogenic gold nanoparticles (GNPs) synthesized through the mediation of Marsilea quadrifolia (M. quadrifolia) methanol extract on 3T3-L1 adipocytes. The biosynthesized GNPs were characterized by UV visible spectrophotometry and FTIR. Simultaneously, the nature, stability, and morpholog ...
... Increased loading of nitrogen (N) and phosphorus (P) from agricultural and urban intensification has led to severe degradation of inland and coastal waters. Lakes, reservoirs, and wetlands (lentic systems) retain these nutrients, thus regulating their delivery to downstream waters. While the processes controlling N and P retention are relatively well‐known, there is a lack of quantitative understa ...
... Azospirillum lipoferum Sp59b microbial cells were immobilized on the surface of thin polystyrene films modified in plasma from a high-frequency discharge of argon (13.56 MHz). The optimal conditions for immobilization, under which cell activity was maintained with respect to specific bacteriophages, were established. It was shown that it is possible to record the interaction of immobilized microbi ...
catalysts; catalytic activity; chemical reactions; dibenzofuran; ligands; phenanthrenes
Abstract:
... Bis(amidinate) ligands attached to rigid dibenzofuran and phenanthrene backbones have been introduced into lanthanide chemistry for the first time. Depending on the ligand and on the lanthanide source, either bi- and tetra-metallic macrocycles or monometallic complexes were obtained. The mono- and bi-metallic systems were compared in terms of their reactivity in the intramolecular hydroamination r ...
... The spatial variations in the elemental and stable carbon, nitrogen, and sulphur isotope composition of bladder wrack (Fucus vesiculosus) growing along the shore line of the semi-enclosed urbanized Kiel Fjord (western Baltic Sea) was investigated at more than 60 sites. The analyses of the carbon-nitrogen-sulphur (CNS) stoichiometry and C and N stable isotope signature of F. vesiculosus displayed s ...
... Dinoflagellates are prolific producers of polyketide compounds, many of which are potent toxins with adverse impacts on human and marine animal health. To identify polyketide synthase (PKS) genes in the brevetoxin‐producing dinoflagellate, Karenia brevis, we assembled a transcriptome from 595 million Illumina reads, sampled under different growth conditions. The assembly included 125,687 transcrip ...
acoustics; research; rivers; suspended sediment; water; British Columbia
Abstract:
... The use of “off‐the‐shelf” acoustic Doppler velocity profilers (ADCPs) to estimate suspended sediment concentration and grain‐size in rivers requires robust methods to estimate sound attenuation by suspended sediment. Theoretical estimates of sediment attenuation require a priori knowledge of the concentration and grain‐size distribution (GSD), making the method impractical to apply in routine mon ...
... On the Qinghai–Tibetan Plateau, isotopic signatures in soil–atmosphere CH ₄ fluxes were investigated in nine grasslands and three wetlands. In the grasslands, the fractionation factor for soil CH ₄ uptake, α ₛₒᵢₗ, was much smaller than the usually reported value of 0.9975–1.0095. Stepwise multiple variation analysis indicates that α ₛₒᵢₗ is higher for higher soil water contents but is lower for hi ...
... Recently, utilizing CO₂ as a methylation reagent to construct functional chemicals has attracted significant attention. However, the conversion of CO₂ is still a challenge due to its inherent inertness. In this study, we have developed a catalyst-free N-methylation of amines to prepare numerous methylamines using CO₂ as a methyl source. By utilizing 2 eq. PhSiH₃ as the reductant, amines could unde ...
... A series of crown-jewel Pd-based bimetallic nanostructures with tunable composition are fabricated inside the pores of an MOF via a hydride-induced-reduction strategy, exhibiting high activity and stability in the hydrogenation of nitrobenzene. ...
... Approximately half of the Padina (Dictyotales, Phaeophyceae) species mineralize aragonite needles over the adaxial thallus surface, where mineral bands are interspersed with nonmineralized regions along the thallus from the apical to basal end. However, this calcification pattern and the related algal properties are not well understood. Therefore, this work was performed to elucidate a potential r ...
Vaccinia virus; binding capacity; genes; mass spectrometry; proteins
Abstract:
... Identification of viral-host interacting proteins will contribute to understanding of how poxvirus exploits the host cellular machinery. The vaccinia virus gene K7R encodes a conserved protein K7 in most orthopoxviruses. To gain insight into the biology of K7, we investigated the cellular interactome of K7 by GST pulldown coupled with mass spectrometry. The top categories of identified proteins co ...
Whites; databases; frequency distribution; gene frequency; genome; genotype; loci; risk; single nucleotide polymorphism; stroke
Abstract:
... With the aim to determine specific genetic characteristics of the population of Moscow region, we determined allele frequencies of 48 polymorphic loci (SNP) associated with the increased risk of ischemic stroke development. The genotype frequency distribution for all the SNPs corresponds to Hardy–Weinberg equilibrium. Comparison of the allele frequencies with those obtained for Caucasian populatio ...
... The genome of Eptesipoxvirus (EPTV) is the first poxvirus genome isolated from a microbat. The 176,688 nt sequence, which is believed to encompass the complete coding region of the virus, is 67% A+T and is predicted to encode 191 genes. 11 of these genes have no counterpart in GenBank and are therefore unique to EPTV. The presence of a distantly related ortholog of Vaccinia virus F5L in EPTV uncov ...
... The process of leaching of copper-zinc concentrate with a solution containing biogenic iron, which is a product of the metabolism of iron-oxidizing microorganisms, was studied. The dependence of leaching rate of metals on temperature and pH was determined. It was shown that up to 98% of zinc and 70% of iron could be removed from the concentrate, while up to 7 and 4 g/L of zinc and copper, respecti ...
advection; bitumen; byproducts; dissolved chlorides; geochemistry; lakes; mass transfer; oils; research; water
Abstract:
... Fluid fine tailings (FFT) are a principal by‐product of the bitumen extraction process at oil sands mines. Base Mine Lake (BML)—the first full‐scale demonstration oil sands end pit lake (EPL)—contains approximately 1.9 × 10⁸ m³ of FFT stored under a water cover within a decommissioned mine pit. Chemical mass transfer from the FFT to the water cover can occur via two key processes: (1) advection‐di ...
... Landouzy–Dejerine facioscapulohumeral muscular dystrophy (FSHD) is one of the most common hereditary myodystrophies. A study of the genetic nature of the disease, which has an autosomal dominant mode of inheritance, is extremely interesting and revealing. A unique structure of D4Z4 macrosatellite repeats found in the 4q35 region was originally characterized by a decrease in the number of repeats i ...
... Using collars for measuring soil respiration and its component fluxes in closed chamber systems relies on two main assumptions. Firstly, it is assumed that shallow collars prevent lateral soil gas leakage beneath the chamber’s walls and the underestimation of soil CO₂ fluxes, and secondly, the insertion of deeper collars excises all living roots and the autotrophic flux is eliminated. It was hypot ...
DNA; DNA barcoding; Phenacoccus solenopsis; biological control; biological control agents; cotton; genes; haplotypes; insects; mitochondria; nucleotide sequences; parasitoids; France; Pakistan
Abstract:
... Because correct identification of insects is crucial for pest management involving chemical or biological control agents, we have used a molecular approach to identify and characterize specimens of the cotton pest Phenacoccus solenopsis Tinsley (Sternorrhyncha: Pseudococcidae) present in different regions of Pakistan. The specimens were analyzed through DNA sequence analysis of their mitochondrial ...
... One natural recombinant subgroup B avian leukosis virus (ALV) with a subgroup J-like U3 region was isolated from commercial native chickens that experienced disease in 2014 and named GX14FF03. GX14FF03 was isolated by DF-1 cell culture and then identified with ELISA detection of avian leukosis virus p27 group-specific antigen, the detection of subtype specific PCR, and indirect immunofluorescence ...
air; carbon dioxide; rural areas; spectroscopy; stable isotopes; typhoons; urban areas; water vapor; Japan
Abstract:
... Isotope ratios of carbon dioxide and water vapour in the near-surface air were continuously measured for one month in an urban area of the city of Nagoya in central Japan in September 2010 using laser spectroscopic techniques. During the passages of a typhoon and a stationary front in the observation period, remarkable changes in the isotope ratios of CO ₂ and water vapour were observed. The isoto ...
Lewis acids; ammonium salts; bromides; catalysts; catalytic activity; chemical reactions; enantioselectivity; esters
Abstract:
... Epoxide desymmetrizations by bromide are very rare despite the large synthetic potential of chiral bromohydrins. Herein we present a new concept for epoxide desymmetrizations in which a bifunctional Lewis acid/ammonium salt catalyst allows for efficient enantioselective epoxide ring openings by Br⁻. With acetylbromide as a Br⁻ source bromohydrin esters are formed. ...
... Herein is reported the mild and general coupling of amine/ether C(sp³)–H bonds with various kinds of C(sp²)–O electrophiles with high selectivity and efficiency. Valuable allylic/benzylic amines are generated in moderate to excellent yields. The utility of this transformation is demonstrated by a broad substrate scope (>50 examples), good functional group tolerance and facile product modification. ...
alpha-tocopherol; chemical bonding; mass spectrometry; nuclear magnetic resonance spectroscopy; water solubility
Abstract:
... Covalent, ionic, and ionic-covalent conjugates of trolox and α-tocopherol with 1-aminoadamantane were synthesized. Their structures were elucidated using mass spectrometry and IR, PMR, and ¹³C NMR spectroscopy. The water solubility of the ionic trolox conjugates with 1-aminoadamantane increased by 2–24 times as compared with that of trolox whereas that of the α-tocopherol succinate conjugates rema ...
... The study of the poultry needs in basic nutrients allowed the development of a scheme for obtaining feed polypeptides (“polypeptide cassettes”) enriched with L-amino acids, which are necessary for the metabolism of birds. The amino acid and nucleotide profiles of about 500 bioinformation sequences of thermostable plant proteins and archaea were studied, on the basis of which candidate sequences we ...
... Microalgae cultivation for biofuels production and other applications has gained considerable interest recently. Despite their simple structures, microalgae can accumulate significant amounts of neutral lipids per dry cell weight compared to other energy crops. Neochloris oleoabundans is a promising microalga known for its high lipid content and biomass growth rate compared to other species cultiv ...
Dinophyceae; chloroplasts; estuaries; hairs; microtubules; nucleotide sequences; peduncle; phylogeny; ribosomal DNA; scanning electron microscopy; transmission electron microscopy
Abstract:
... A new marine woloszynskioid dinoflagellate Dactylodinium pterobelotum gen. et sp. nov., collected from a southern Vietnamese estuary, was described on the basis of LM, SEM, and TEM, and molecular phylogeny inferred from rDNA sequences. This species had the smallest number of amphiesmal vesicles (5 latitudinal series) in woloszynskioid dinoflagellates assigned to the Suessiaceae and Borghiellaceae. ...
Culicoides; adults; females; males; new species; pupae; scientific illustration; taxon descriptions; taxonomic keys; France; India
Abstract:
... Pupa, and adult male and female of Culicoides pseudosimilis n. sp., and pupae of two known species, C. selangorensis Wirth & Hubert, and C. dryadeus Wirth & Hubert, are described and illustrated. An identification key to the Indian species of Clavipalpis and Shermani species group of male Culicoides is also provided. ...
... We describe a novel species of torque teno mini virus called TTMV-204, which was isolated from the gingival epithelium of patients with periodontitis and characterized using viral metagenomics. The sequence of the full genome is 2824 nt in length. Phylogenetic analysis and genetic analyses show classic Betatorquevirus species organization with less than 40% amino acid similarity in ORF1. The preva ...