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... Genetically modified (GM) plants have potential benefits in modern agriculture such as increasing yields and decreasing the use of chemicals. However, GM plants may cause unintended consequences of gene flow to indigenous plants or microbes and the detrimental effects on non-target organisms such as soil microbial communities. To investigate these possible unintended effects of GM plants, we condu ...
... Cassava brown streak disease (CBSD) is the most important virus disease of cassava and a major food security threat in Africa. Yearly economic losses of up to $100 million USD have been attributed to CBSD. The lack of information on plant-virus interactions has restricted progress in breeding for CBSD resistance. Virus quantification is becoming a major tool for the quick and reliable assessment o ...
... Rabbit haemorrhagic disease virus (RHDV) is a highly infectious pathogen that causes high mortality in wild and domestic rabbits. RHDV could be divided into two subtypes, classic RHDV and RHDVa, which present clear genetic, antigenic, and epidemiological differences. To further understand the nature of the diversity, we performed a genome-wide evolutionary study on the classic RHDV and RHDVa isola ...
... Cytotoxic T-lymphocyte antigen 4 (CTLA-4) is recognized as a critical inhibitory regulator of T-cell proliferation and activation, opposing the action of CD28-mediated co-stimulation. Interfering or blocking CTLA-4 can result in continuous T-cell activation required for the full immune response to pathogenic microbes and vaccines. To test if nucleic acid-based CTLA-4 inhibitors could be developed ...
Banana bract mosaic virus; Banana bunchy top virus; Banana streak virus complex; Cucumber mosaic virus; Musa; National Center for Biotechnology Information; bananas; coat proteins; codons; crops; livelihood; phylogeny; plant viruses; India
Abstract:
... The banana is one of the world’s most important livelihood crops. Banana plants are principally infected by four virus species, Banana bunchy top virus (genus Babuvirus), Cucumber mosaic virus (genus Cucumovirus), Banana streak virus (genus Badnavirus) and Banana bract mosaic virus (genus Potyvirus). The objective of this study is to understand the codon usage pattern and phylogeny of coat protein ...
... Despite being the first closterovirus documented in grapevines (Vitis sp.), the molecular biology of Grapevine leafroll-associated virus 1 (GLRaV-1, genus Ampelovirus, family Closteroviridae) is still in its infancy. In this study, the complete genome sequence of two GLRaV-1 isolates was determined to be 18,731 (isolate WA-CH) and 18,946 (isolate WA-PN) nucleotides (nt). The genome of WA-CH and WA ...
Apple chlorotic leaf spot virus; Apple mosaic virus; Apple stem grooving virus; Apple stem pitting virus; Malus domestica; Prunus necrotic ringspot virus; RNA; amino acid sequences; apples; coat proteins; genome; high-throughput nucleotide sequencing; leaves; orchards; phylogeny; plant diseases and disorders; plant viruses; reverse transcriptase polymerase chain reaction; surveys; trees; viruses; China; Japan
Abstract:
... The causal agent of apple mosaic disease has been previously thought to be solely caused by apple mosaic virus (ApMV). In this study, we report that a novel ilarvirus is also associated with apple mosaic disease. Next-generation sequencing analysis of an apple tree showing mosaic symptoms revealed that the tree was infected with three apple latent viruses (apple stem pitting virus, apple stem groo ...
... The aim of this study was to evaluate the serologic status of domestic pigeons not infected and asymptomatically infected with the pigeon circovirus (PiCV) with the use of an enzyme-linked assay based on PiCV recombinant capsid protein as a plate antigen. Recombinant PiCV capsid protein was produced by transforming E. coli BL21 (DE3) Rosetta colonies with expression plasmids.Blood samples and cloa ...
Michal Pyc; Yingqi Cai; Satinder K. Gidda; Olga Yurchenko; Sunjung Park; Franziska K. Kretzschmar; Till Ischebeck; Oliver Valerius; Gerhard H. Braus; Kent D. Chapman; John M. Dyer; Robert T. Mullen
Arabidopsis; baits; biogenesis; coat proteins; droplet size; droplets; endoplasmic reticulum; homeostasis; knockout mutants; leaves; lipid content; phenotype; seed oils; seeds; surfactants; tissues; transfer DNA; triacylglycerols; two hybrid system techniques
Abstract:
... Cytoplasmic lipid droplets (LDs) are found in all types of plant cells; they are derived from the endoplasmic reticulum and function as a repository for neutral lipids, as well as serving in lipid remodelling and signalling. However, the mechanisms underlying the formation, steady‐state maintenance and turnover of plant LDs, particularly in non‐seed tissues, are relatively unknown. Previously, we ...
... The ADP ribosylation factor (Arf) and the coat protein complex I (COPI) are involved in vesicle transport. Together with GTPase‐activating proteins (ArfGAPs) and guanine exchange factors (ArfGEFs) that regulate the activity of Arf, they govern vesicle formation, COPI trafficking and the maintenance of the Golgi complex. In an ongoing effort to study the role of membrane trafficking in pathogenesis ...
... The incidence of Groundnut rosette assistor virus (GRAV) in farmers’ fields and sequence diversity of groundnut rosette disease (GRD) agents were assessed in the three northern groundnut production regions of Ghana. GRAV incidence was high (69.5 to 75.0%) but not significantly different between the regions. Nucleotide sequencing of GRAV coat protein (CP) gene revealed 99–100% identity among the Gh ...
... Based on second generation sequencing (MiSeq platform, Illumina), we determined the genetic diversity of T4-like bacteriophages of the family Myoviridae by analysing fragments of the major capsid protein gene g23 in the plankton of Lake Baikal. The sampling depth in our study was significantly higher than in those obtained by the Sanger method before. We obtained 33 701 sequences of the g23 gene f ...
... Progressive research has been recently made in dissecting the molecular biology of Betanodavirus life cycle, the causative pathogen of viral encephalopathy and retinopathy in economic important marine fish species. Establishment of betanodavirus infectious clone allows the manipulation of virus genome for functional genomic study, which elucidates the biological event of the viral life cycle at mo ...
... Tobacco mosaic virus (TMV) is an important plant virus that can cause considerable crop loss. Our group synthesized a series of enantiomeric α-aminophosphonate derivatives with high anti-TMV activities. The activity of (R)-diphenyl-1-(4-methylbenzothiazole-2-amino)-1-(thiphene-2-yl)-methylphosphonate (Q-R) was found to be superior to that of (S)-diphenyl-1-(4-methyl benzothiazole-2-amino)-1-(thiph ...
... During infection host histo-blood group antigens (HBGAs) act as attachment factors that interact with rabbit hemorrhagic disease virus (RHDV) and participate in the infectious process. In the present study, baculovirus expressing recombinant RHDV capsid protein (VP60r) as a vaccine immunogen was used to test its antigenicity and immunogenicity via immunization experiments. Each group of rabbits im ...
... A new partitivirus named Lentinula edodes partitivirus 1 (LePV1) was isolated from a diseased L. edodes strain with severe degeneration of the mycelium and imperfect browning in bag cultures. The nucleotide sequences of LePV1 dsRNA-1 and dsRNA-2 were determined; they were 2,382 bp and 2,245 bp in length, and each contained a single ORF encoding RNA-dependent RNA polymerase (RdRp) and coat protein ...
... Zucchini yellow mosaic virus (ZYMV) causes one of the most destructive diseases of cucurbits worldwide. Although the virus has been present in China for more than 25 years, there is little information about biological and molecular traits of ZYMV isolates from China. This study aimed to characterize 26 ZYMV isolates from Xinjiang, China based on pathotypes, molecular variability and host range. Ph ...
Potexvirus; RNA-directed RNA polymerase; Senna occidentalis; bioassays; coat proteins; genes; nucleotides; open reading frames; plant diseases and disorders; plant viruses; polyclonal antibodies; seeds; viruses; Brazil
Abstract:
... In this work, we report the complete genome sequence of, production of polyclonal antibodies against, and development of biological assays for a putative new potexvirus, named senna mosaic virus (SenMV), found infecting Senna occidentalis in the state of São Paulo, Brazil. The complete genome sequence of SenMV comprises 6775 nucleotides excluding the poly(A) tail. The genome organization is simila ...
... The family Polyomaviridae encompasses non-enveloped viruses with a circular dsDNA genome that is typically approximately 5000bp in length. Originally isolated from mammals, polyomavirus sequences have now been detected in invertebrates, fish, amphibians, reptiles and birds, although it remains to be determined whether all these animals are genuine hosts. The genomes of all polyomaviruses encode at ...
... We isolated two distinct viruses from mosquitoes collected in Bustos, Bulacan province, Philippines, in 2009. These viruses show rapid replication and strong cytopathic effects in mosquito C6/36 cells. Whole-genome analysis of these viruses demonstrated that both viruses belong to the negevirus group. One of the viruses, from Culex vishunui mosquitoes, is a new strain of Negev virus. The other vir ...