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... Outer membrane protein A (OmpA) plays multiple roles in the physiology and pathogenesis of the zoonotic pathogen enterohemorrhagic Escherichia coli (EHEC). The N-terminus of OmpA forms a transmembrane domain (OmpA™), and the roles of this domain in bacterial pathogenesis have been well studied. However, how its C-terminal domain (OmpAper), which is located at the periplasmic space in the bacterial ...
... Motivation: Horizontal gene transfer (HGT) is a fundamental mechanism that enables organisms such as bacteria to directly transfer genetic material between distant species. This way, bacteria can acquire new traits such as antibiotic resistance or pathogenic toxins. Current bioinformatics approaches focus on the detection of past HGT events by exploring phylogenetic trees or genome composition inc ...
... We investigated a bacterial sample preparation procedure for single-cell studies. In the present study, we examined whether single bacterial cells obtained via 10-fold dilution followed a theoretical Poisson distribution. Four serotypes of Salmonella enterica, three serotypes of enterohaemorrhagic Escherichia coli and one serotype of Listeria monocytogenes were used as sample bacteria. An inoculum ...
... Contamination of beef products by Shiga toxin–producing Escherichia coli is a concern for food safety with a particular subset, the enterohemorrhagic E. coli (EHEC), being the most relevant to human disease. To mitigate food safety risks, preharvest intervention strategies have been implemented with the aim to reduce EHEC in cattle. One class of interventions that has been widely used in feedlots ...
... The effects of marinades on Listeria monocytogenes and enterohaemorrhagic Escherichia coli were investigated in pressure treated beef steaks. Meat was treated with 600MPa or 450MPa. Marinades did not enhance pressure inactivation of E. coli in beef steaks and marinades prevented pressure-induced sublethal injury in L. monocytogenes. Membrane-active essential oils carvacrol and thymol, and thiol-re ...
... Dry-fermented sausages (DFSs) have been linked to several serious foodborne outbreaks of enterohemorrhagic Escherichia coli (EHEC). The ability of pathogens to utilize adaptive responses to different stressful conditions intended to control their growth in foods, food preparation and production processes may enhance their survival. In certain cases, induced tolerance to one type of stress may lead ...
animal wastes; bacteria; body weight changes; diarrhea; drinking water; enterohemorrhagic Escherichia coli; enteropathogenic Escherichia coli; feces; grazing; grazing lands; haptoglobins; lambs; leukocyte count; leukocytes; mineral content; minerals; nitrogen; risk; streams; water pollution; water quality
Abstract:
... Water pollution from animal waste, and its influence on grazing animals, is a current concern regarding Mongolian grazing lands. We allocated 32 free-grazing lambs to four groups and provided each with water from a different source (upper stream, lower stream, well, and pond) for 49 days. We recorded the amount of water consumed by the lambs, as well as their body weight, white blood cell count, a ...
digestive tract mucosa; endosomes; enterohemorrhagic Escherichia coli; enteropathogenic Escherichia coli; guanosinetriphosphatase; pathogens; receptors; type III secretion system
Abstract:
... Enteropathogenic and enterohaemorrhagic Escherichia coli (EPEC/EHEC) manipulate a plethora of host cell processes to establish infection of the gut mucosa. This manipulation is achieved via the injection of bacterial effector proteins into host cells using a Type III secretion system. We have previously reported that the conserved EHEC and EPEC effector EspG disrupts recycling endosome function, r ...
... Within 2 months, two water sources in a karst area in Switzerland were sampled 9 times each, and analyzed by real-time PCR for 6 EHEC O-types, Shiga-like-toxin (stx1 and stx2) and intimin (eae) genes. With the exception of O111, 5 O-types were recorded regularly and at high frequencies (O26: 33.3 %; O157: 33.3 %; O104: 66.6 %; O103: 72.2 %; O145: 94.4 %). Genes for Shiga-like-toxins and intimin we ...
... This study evaluated the performance of a new commercially available multiplex real-time PCR kit Amplidiag® Bacterial GE in the systematic screening of bacterial pathogens causing gastroenteritis. Stool samples from 1168 patients were analyzed with Amplidiag® Bacterial GE, stool culture, and molecular reference tests, and the sensitivity and specificity of Amplidiag® Bacterial GE were determined b ...
Bavo Verhaegen; Inge Van Damme; Marc Heyndrickx; Nadine Botteldoorn; Mohamed Elhadidy; Karen Verstraete; Katelijne Dierick; Sarah Denayer; Lieven De Zutter; Koen De Reu
... Shiga toxin-producing Escherichia coli (STEC) remains a major foodborne pathogen of concern across the globe. Rapid detection and isolation of this pathogen is of great importance for public health reasons.In this study the detection and isolation of four non-O157 STEC strains (O26, O103, O111, O145) from different artificially contaminated matrices, namely ground (minced) beef, cattle carcass swa ...
... As a global transcriptional regulator, H-NS, the histone-like nucleoid-associated DNA-binding and bridging protein, plays a wide range of biological roles in bacteria. In order to determine the role of H-NS in regulating gene transcription and further find out the biological significance of this protein in Enterohemorrhagic Escherichia coli (EHEC), we conducted transcriptome analysis of hns mutant ...
Listeria; Salmonella; animal manures; animal pathogenic bacteria; antibiotic resistance; biosolids; climate; composted manure; enterohemorrhagic Escherichia coli; feedstocks; food pathogens; fruits; genes; green leafy vegetables; guidelines; manure spreading; physicochemical properties; regrowth; soil amendments; soil chemical properties; soil physical properties; United States
Abstract:
... Biological soil amendments (BSAs) such as manure and compost are frequently used as organic fertilizers to improve the physical and chemical properties of soils. However, BSAs have been known to be a reservoir for enteric bacterial pathogens such as enterohemorrhagic Escherichia coli (EHEC), Salmonella spp., and Listeria spp. There are numerous mechanisms by which manure may transfer pathogens to ...
... Probiotics are usually isolated from the gastrointestinal tract of humans and animals. The search of probiotics in human milk is a recent field of research, as the existence of the human milk microbiome was discovered only about a decade ago. To our knowledge, no reports regarding the potential probiotic effect of bacteria from swine milk have been published. In this work, we isolated several lact ...
... In the present study we investigated the occurrence of Escherichia coli strains harboring the gene encoding enterohemorrhagic E. coli hemolysin (EHEC-HlyA) in cattle and the association of this gene with various diarrheagenic E. coli (DEC) pathotypes. First, the bovine E. coli isolates were screened for EHEC-hlyA gene by PCR, and then they were characterized for the phylogenetic groups and the pre ...
... Quorum sensing is a cell-to-cell communication mechanism leading to differential gene expression in response to high population density. The autoinducer-1 (AI-1) type quorum sensing system is incomplete in Escherichia coli and Salmonella due to the lack of the AI-1 synthase (LuxI homolog) responsible for acyl homoserine lactone (AHL) synthesis. However, these bacteria encode the AHL receptor SdiA ...
... The primary objective of this study was to determine the prevalence and level of enterohemorrhagic Escherichia coli (EHEC) O26, O45, O103, O111, O121, and O145 (collectively EHEC-6) plus EHEC O157 in fecal, hide, and preintervention carcass surface samples from culled dairy cows. Matched samples (n = 300) were collected from 100 cows at harvest and tested by a culture-based method and two molecula ...
Law Robyn J.; Law Hong T.; Scurll Joshua M.; Scholz Roland; Santos Andrew S.; Shames Stephanie R.; Deng Wanyin; Croxen Matthew A.; Li Yuling; de Hoog Carmen L.; van der Heijden Joris; Foster Leonard J.; Guttman Julian A.; Finlay B. Brett
digestive system diseases; enterohemorrhagic Escherichia coli; enteropathogenic Escherichia coli; mass spectrometry; mechanism of action; microtubules; morbidity; mortality; mutants; pathogens; physiological transport; proteome; proteomics; type III secretion system; virulence
Abstract:
... Enteropathogenic and enterohemorrhagic Escherichia coli cause enteric diseases resulting in significant morbidity and mortality worldwide. These pathogens remain extracellular and translocate a set of type III secreted effector proteins into host cells to promote bacterial virulence. Effectors manipulate host cell pathways to facilitate infection by interacting with a variety of host targets, yet ...
... Biofilm formation on abiotic surfaces in fresh produce processing facilities may play a role in foodborne outbreaks by providing protective microniches for pathogenic bacteria. Our previous study showed that a strain of Ralstonia insidiosa isolated from a fresh produce processing plant could enhance the incorporation of Escherichia coli O15:H7 in biofilms under various environmental conditions. Th ...
... Food-borne Shiga toxin-producing Escherichia coli (STEC) O113:H21 strain TS18/08, that has previously been isolated from mixed minced meat, harbors the Shiga toxin (Stx) encoding allele stx2a, the plasmid-located subtilase cytotoxin encoding allele subAB1 and the cytolethal distending toxin type V encoding gene cdt-V. In the current study, it could be shown that each of these toxin genes was trans ...
... AIMS: To assay the combination of phage and probiotics against EHEC in vitro on infected Hep‐2 cells. METHODS AND RESULTS: Phage and probiotics treatments on EHEC O157:H7‐infected Hep‐2 cells were assayed individually or combined. The effect of freeze‐drying on phage and probiotic antimicrobial activity was also studied. While treatment with phage alone increased cell detachment caused by EHEC inf ...
... Shiga Toxin (Stx) is one of the major virulence factors produced by Shiga Toxin-producing E. coli (STEC) that cause severe human intestinal diseases. Although a few commercial assays for Stxs are available, they only detect a subset of Stxs. In this study, two new immunoassays, Abraxis Stx1 and Stx2, were evaluated and compared with the widely used Premier EHEC kit using the same set of standards ...
... Close contact of vegetables with soil, polluted water, and animal manure and unsanitary conditions during processing of restaurant salads led us to study the distribution of virulence factors, O-serogroups, and antibiotic resistance properties in Shiga toxigenic Escherichia coli (STEC) isolated from vegetables and salads. Samples of vegetables and salad (n = 420) were collected and evaluated for t ...
... High pressure (HP) processing, typically at 600MPa in combination with mild temperatures, makes it possible to produce safe and high quality foods while inactivating pathogenic microorganisms such as Enterohaemorrhagic Escherichia coli (EHEC). In the last decade much research was conducted to understand the inactivation of spores at high pressures and elevated temperatures. Bacterial spore inactiv ...