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... ATP-binding cassette (ABC) transporters mediate active efflux of natural and synthetic toxicants and are considered to be important for drug tolerance in microorganisms. In biological control agents (BCA), ABC transporters can play important roles in antagonism by providing protection against toxins derived from the fungal prey and by mediating the secretion of endogenous toxins. In the present st ...
Macrocentrus; Magnoliopsida; Ostrinia nubilalis; Trichogramma brassicae; adults; biological control; biological control agents; cocoons; corn; economic analysis; eggs; environmental impact; females; longevity; males; oviposition; parasitism; parasitoids; progeny; Asia; Europe; North America
Abstract:
... We examined whether Macrocentrus cingulum (Hymenoptera: Braconidae) of Asian origin could serve as a biological control agent of the maize pest Ostrinia nubilalis (Lepidoptera: Crambidae) in Europe. M. cingulum is already present in Europe, where it does not parasitize O. nubilalis but Ostriniascapulalis, a related species feeding on wild dicotyledons. In contrast, M. cingulum have been imported f ...
... Orius sauteri (Poppius) is an important hemipterous predator that has been mass-reared for biological control of numerous pests in protected crop-production systems. To find a good oviposition substrate for mass-rearing this predator under insectary conditions [25°C, 65 ± 5% relative humidity, and a photoperiod of 16:8 (L:D) h], we compared kidney bean, soybean, broad bean sprouts and fresh leaves ...
Castanea; Dryocosmus kuriphilus; Megastigmus; Torymus; biological control; natural enemies; parasitoids; United States
Abstract:
... The Asian chestnut gall wasp, Dryocosmus kuriphilus (Hymenoptera: Cynipidae), is a globally invasive pest of Castanea. Population regulators and geographic range expansion of the Asian chestnut gall wasp in North America with focus on natural enemy recruitment have been evaluated. After the initial discovery of the gall wasp in the U.S., there were biological control releases that included Megasti ...
... The objectives of this work were to assess the optimum conditions for induction of acid tolerance in the marine yeast Rhodosporidium paludigenum and evaluate the biocontrol activity of non-adapted and acid-adapted yeasts in controlling apple blue mold caused by Penicillium expansum. R. paludigenum grown in malic and lactic acid treatments were stimulated after 12 h incubation. Moreover, medium mod ...
... Jasmonates (JAs) are lipid-derived compounds acting as key signaling compounds in plant stress responses and development. The JA co-receptor complex and several enzymes of JA biosynthesis have been crystallized, and various JA signal transduction pathways including cross-talk to most of the plant hormones have been intensively studied. Defense to herbivores and necrotrophic pathogens are mediated ...
Filippe Elias de Freitas Soares; José Humberto de Queiróz; Fabio Ribeiro Braga; Alexandre de Oliveira Tavela; Juliana Milani Araújo; Leandro Abreu da Fonseca; Angélica de Souza Gouveia; Jackson Victor de Araújo
... The ovicidal action of the nematophagous fungus Pochonia chlamydosporia (VC4) was evaluated on Dioctophyma renale eggs under laboratory conditions (Assay A). Next, the enzymatic action of proteases and chitinases produced by P. chlamydosporia (VC4) was evaluated on D. renale eggs, under laboratory conditions (Assay B). At the end of the experiment, there was difference (p < 0.01) in the destructio ...
... Adenylate cyclase (AC) is a core element of cAMP signalling network. Here we show functional diversity and differentiation of Beauveria bassiana AC (BbAC) and Metarhizium robertsii AC (MrAC). Severe growth defects occurred in ΔBbAC and ΔMrAC grown on nutrition-rich SDAY and several minimal media but were largely alleviated by adding cAMP to SDAY. Conidial yield increased greatly in ΔBbAC but decre ...
... Mosquito-borne diseases with an economic impact create loss in commercial and labor outputs, particularly in countries with tropical and subtropical climates. Mosquito control is facing a threat because of the emergence of resistance to synthetic insecticides. Extracts from plants may be alternative sources of mosquito control agents because they constitute a rich source of bioactive compounds tha ...
... Entomopathogenic fungi, such as Beauveria bassiana and Metarhizium anisopliae, are environmentally friendly biocontrol agents (BCAs) against various arthropod pests. We provide an overview to the past-decade advances in fungal BCA research and application in China. Since 1960s, fungal BCAs have been mass-produced for application and at present, thousands of tons of their formulations are annually ...
... Chromium (Cr) may cause losses in the yield of field plant, which is one of the favorite habitats of Bacillus thuringiensis (Bt). The purposes of our study were to assess the Cr(VI)-resistance and Cr(VI)-reducing abilities of an indigenous soil isolate of Bt and to determine the factors governing Cr(VI) reduction. Towards this end a novel dichromate-reducing Bt BRC-ZYR2, characterized with insecti ...
... Biological control of the fungus Aspergillus flavus has been shown to be effective in reducing aflatoxin contamination in corn. This study compared field application of a bioplastic-based formulation for delivering atoxigenic A. flavus isolates in Northern Italy and the Mississippi Delta.Due to an extremely hot and dry summer at the Italy site in 2012, aflatoxin contamination was approximately sev ...
... The fruit fly, Bactrocera invadens is an alien invasive pest of mango in Africa. The predatory African weaver ant, Oecophylla longinoda is being promoted for managing B. invadens and classical biological control with the Opiine parasitoid, Fopius arisanus is also underway. The current study investigated interactions between these two natural enemies. Specifically we assessed whether semiochemicals ...
Tyto alba; agricultural land; biological control; data collection; economic policy; models; nesting; predation; profitability; rodent control; rodenticides; rodents; vertebrate pests; Israel
Abstract:
... We develop a model to evaluate the profitability of controlling rodent damage by placing barn owl nesting boxes in agricultural areas. The model incorporates the spatial patterns of barn owl predation pressure on rodents, and the impact of this predation pressure on nesting choices and agricultural output. We apply the model to data collected in Israel and find the installation of nesting boxes pr ...
... Lecanicillium lecanii has been used in the biological control of several insects in agricultural practice. Since the gene manipulation tools for this entomopathogenic fungus have not been sufficiently developed, Agrobacterium tumefaciens-mediated transformation (ATMT) in L. lecanii was investigated in this study, using the wild-type isolate FZ9906 as a progenitor strain and the hygromycin B resist ...
... The forecasted 9.1 billion population in 2050 will require an increase in food production for an additional two billion people. There is thus an active debate on new farming practices that could produce more food in a sustainable way. Here, we list agroecological cropping practices in temperate areas. We classify practices according to efficiency, substitution, and redesign. We analyse their advan ...
Neoseiulus californicus; Phytoseiulus persimilis; air temperature; biological control; cold storage; energy requirements; females; habitats; predatory mites; vapor pressure; water vapor
Abstract:
... BACKGROUND: Humidity‐controlled cold storage, in which the water vapour pressure is saturated, can prolong the survival of the predatory mites Neoseiulus californicus (McGregor) and Phytoseiulus persimilis Athias‐Henriot (Acari: Phytoseiidae). However, information on the optimum air temperature for long‐term storage by this method is limited. The authors evaluated the survival of mated adult femal ...
... Conidiobolus thromboides is an entomophthoralean fungus with potential as a biological control agent of aphids. However, its application in biological control is limited due to its formulation requirements. The objective of this study was to develop and optimise a novel air-extrusion method to embed C. thromboides hyphae at high density in alginate pellets. An orthogonal experimental design was us ...
... Bacillus thuringiensis subsp. israelensis (Bti) is widely used for the biological control of mosquito populations. However, the mechanism of Bti toxins is still not fully understood. To further elucidate the mechanism of Bti toxins, we developed an Aedes aegypti resistant strain that shows high-level resistance to Cry11Aa toxin. After 27 selections with Cry11Aa toxin, the larvae showed a 124-fold ...
... Tiphia vernalis Rohwer and Tiphia popilliavora Rohwer were introduced as biocontrol agents against Japanese beetles (Popillia japonica Newman) and oriental beetles (Anomala orientalis Waterhouse). Studies have shown that under field conditions, T. vernalis parasitize Japanese beetles more often than oriental beetles. This study was done to understand how tiphiid wasps handle the two different host ...
... We assembled antennal transcriptomes of pest Monochamus alternatus and its parasitoid Dastarcus helophoroides to identify the members of the major chemosensory multi-gene families. Gene ontology (GO) annotation indicated that the relative abundance of transcripts associated with specific GO terms was highly similar in the two species. In chemosensory gene families, we identified 52 transcripts enc ...
... In African cocoa agroforestry systems, ants represent the most important part of arboreal arthropod biomass and play a crucial role in ecosystem functioning. The more abundant are supposed to be involved in crop pest regulation. However, the lack of knowledge about ecological interactions between ants and their habitat, including other ant species of the community, leaded to contradictory views on ...
... The relationship between two natural enemies of Coccus viridis (green coffee scale), an important hempiteran coffee pest was determined using a combination of experimental and observational approaches. Adult and larval forms of Azya orbigera, a coccinellid beetle predator were included on leaves of coffee plants with healthy scale populations resulting in lower proportions of scales infected with ...
... This study aimed to develop a biocontrol system for ginseng root rot caused by Fusarium cf. incarnatum.In total, 392 bacteria isolated from ginseng roots and various soils were screened for their antifungal activity against the fungal pathogen, and a bacterial isolate (B2-5) was selected as a promising candidate for the biocontrol because of the strong antagonistic activity of the bacterial cell s ...
... The objective of this study was to isolate and characterize bacterial strains associated with the gut of the hybrid Carniolan honey bee, Apis mellifera carnica, and to determine their in vitro and in vivo potential against Ascosphaera apis, the causal organism of chalkbrood disease, with the purpose of exploring feasible biological control. Six bacterial strains were isolated from healthy worker h ...
... Trichoderma is a well-known antagonist against soilborne plant pathogens. However, the species and even various isolates have different biocontrol potential. To evaluate the antagonistic activities of Trichoderma harzianum , T. harzianum strain T100 (T100), T. viride and T. haematum against Fusarium oxysporum and F. proliferatum , we used dual culture and productions of volatile and non-volatile m ...
Acremonium; Aureobasidium pullulans; Cladosporium; Fusarium culmorum; Fusarium head blight; Penicillium; biological control; biological control agents; cell aggregates; filling period; fungi; greenhouse experimentation; hyphae; pathogens; scanning electron microscopy; seeds; winter wheat
Abstract:
... The effect of the biological control agent Aureobasidium pullulans (de Bary) G. Arnaud on the development of Fusarium head blight (FHB) on winter wheat and kernel contamination with fungi of the genera Fusarium, Acremonium, Cladosporium and Penicillium was analyzed in a greenhouse experiment. Scanning electron microscopy was used to evaluate the distribution of A. pullulans cells and aggregates on ...
... Bakanae disease, caused by Fusarium fujikuroi, is the most important seedborne disease of rice. Biological control and physical treatments can be effective tools to control seedborne diseases. Sixty-two isolates of yeasts and yeast-like fungi were obtained from different rice seeds. Four yeast isolates were selected in dual culture assays for mycelial growth inhibition, and in seed tests for reduc ...
... Membrane biofouling, resulting from biofilm formation on the membrane, has become the main obstacle hindering wider application of membrane technology. Initial biofouling proves to be crucial which involves early stages of microbial adhesion and biofilm formation. Biological control of microbial attachment seems to be a promising strategy due to its high efficiency and eco-friendliness. The presen ...
... Metschnikowiaceae may show natural, strain-dependent antifungal properties, so they are employed in agriculture as natural, safe alternatives to pesticides. With this paper, we documented the ability of the recently described Metschnikowia IHEM 25107 to inhibit growth of several molds, including diverse Aspergillus species and dermatophytes. This biocontrol activity enables pulcherrimin-producing ...
... The antagonistic fungus Simplicillium lamellicola BCP has been developed as a microbial biopesticide that effectively controls the development of various plant diseases caused by both pathogenic bacteria and pathogenic fungi. Antibacterial bioassay-directed fractionation was used to isolate mannosyl lipids from S. lamellicola BCP, and the structures of these compounds were elucidated using spectra ...
... In central Oklahoma, winter canola (Brassica napus L.) has recently become the primary rotational winter crop with wheat (Triticum aestivum L.). Annual aphid pest outbreaks in canola have resulted in widespread application of insecticide. Insect parasitoids, which frequently suppress aphids in nearby wheat, may move to canola because of large numbers of aphids. These parasitoids probably will be e ...
Pseudomonas; Trichoderma koningii; anthracnose; bacterial wilt; biological control; field experimentation; fluorescence; villages; viruses; Indonesia
Abstract:
...
Major diseases on chili in Minahasa are bacterial wilt, anthracnose, and mosaic caused by virus infection. The objectives of this research were to evaluate the effectiveness of two microbes, Trichoderma koningii and fluorescent Pseudomonas, as biocontrol agents for major diseases of chili. Field experiment was conducted in Toure Village, Tompaso Baru, District of Minahasa. The ...
... Although apomixis is the most common form of parthenogenesis in diplodiploid arthropods, it is uncommon in the haplodiploid insect order Hymenoptera. We found a new type of spontaneous apomixis in the Hymenoptera, completely lacking meiosis and the expulsion of polar bodies in egg maturation division, on the thelytokous strain of a parasitoid wasp Meteorus pulchricornis (Wesmael) (Braconidae, Euph ...
... Sporisorium reilianum is the causal agent of head smut in corn, a worldwide disease that causes significant economic losses in the principal areas where maize is cultivated. To attack this problem, resistant commercial hybrids and synthetic fungicide seed treatment have been used, but with low effectiveness. In this study, a native strain of Bacillus subtilis isolated from the soil of corn crops i ...
... Clostridium perfringens is frequently found in food and the environment and produces potent toxins that have a negative impact on both human and animal health and particularly on the poultry industry. Lactobacillus johnsonii FI9785, isolated from the chicken gastrointestinal tract, has been demonstrated to exclude Cl. perfringens in poultry. We have investigated the interaction of wild‐type Lact. ...
... Hydrogen sulfide-producing bacteria (SPB) can spoil raw animal materials and release harmful hydrogen sulfide (H₂ S) gas. The objective of this study was to apply a SPB-specific bacteriophage cocktail to control H₂ S production by SPB in different raw poultry by-products in the laboratory (20, 30, and 37°C) and greenhouse (average temperature 29 to 31°C, humidity 34.8 to 59.8%, and light intensity ...
Castanea; Cryphonectria parasitica; biological control; coppicing; forests; fungi; horticulture; human resources; inoculum; orchards; silvicultural practices; tree age; viruses; Greece
Abstract:
... Since 1963 when chestnut blight was recorded for the first time in Greece, the disease has spread into all 29 chestnut growing prefectures. As a result the national, annual chestnut production was reduced from 18,000 tons in the 1960s to 11,000 tons in 2005. A project for the application of biological control on a nationwide scale was funded and implemented during the period 2007-2009. Hypovirulen ...
... Pseudomonas aureofaciens (30-84) is a phenazine producing bacterium and reported as asuccessful biocontrol agent of some plant fungal pathogens. In the present study, the possibility of biological control of cotton damping-off caused by Rhizoctonia solani (AG-4) through phenazine production by the 30-84 strain, was investigated. In the search for the development of bioformulations of Pa (m) (PhzR– ...
... Successful algal biomass cultivation at scale is a key limiting step for the production of algal biofuels and other high-value products, and crop protection against undesirable biomass losses will be a critically important component of commercialization efforts. If algal biomass production occurs in large, open, outdoor ponds, then it can be expected that invasions of these production facilities b ...
... The eastern foothills of the Cascade Range in Washington State, United States of America are west of the State’s major fruit-producing region. Alders (Alnus Hill (Betulaceae)), an important floristic component of riparian communities in the Cascade Range foothills, were surveyed for leafrolling caterpillars (Lepidoptera: Tortricidae) that might serve as alternate hosts for parasitoids of orchard p ...
... The effect of ascorbic acid (VC) on improving oxidative stress tolerance of Pichia caribbica and biocontrol efficacy against blue mold caused by Penicillium expansum on apples was investigated. P. caribbica showed susceptibility to the oxidative stress in vitro test, and 250 μg/mL VC treatment improved its oxidative stress tolerance. The higher viability exhibited by VC-treated yeast was associat ...
... During extensive surveys in fields heavily infested by broomrape in the Trakya Region‐Turkey, a different new fungus, Aspergillus alliaceus, was isolated from the infected broomrape. It is aimed to investigate whether or not it is really a pathogen for Orobanche. The fungi was exposed to a greenhouse environment in order to assess its pathogenicity and virulence against Orobanche cernua. In additi ...
... In this study we compared the efficiency of two Trichogramma (Hymenoptera: Trichogrammatidae) species, the indigenous T. euproctidis Girault and the cosmopolitan T. achaeae Nagaraja & Nagarkatti, for the biological control of the tomato leaf miner, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae). Three release doses (25, 50 and 75 parasitoids/m²) for each species were tested in a two-year exper ...
... All entomopathogenic fungi infect insects by direct penetration through the cuticle rather than per os through the gut. Genetic transformation can confer fungi with per os virulence. However, unless the recipient isolate is nonpathogenic to the target insect, mortality caused by a transgenic isolate cannot be attributed solely to oral virulence due to the potential for some simultaneous cuticular ...
... The ability of a predator to discriminate against parasitized prey determines the extent of asymmetrical intraguild predation, which is often crucial for the outcome of biological control. Anagyrus nr. pseudococci (Girault) (Hymenoptera: Encyrtidae), a parasitoid of the citrus mealybug, Planococcus citri (Risso) (Hemiptera: Pseudococcidae), suffers from intraguild predation by coccinellids occurri ...
parasitoids; Lepidoptera; Hymenoptera; moths; natural enemies; Tachinidae; flowers; herbivores; crop yield; Malaise traps; alternative hosts; Angiospermae; biological control
Abstract:
... Flowering plants added to agricultural environments can provide food, shelter and alternative hosts for natural enemies and so increase crop yields. However, these same resources might be exploited by certain pests. Twelve species of plants known to attract parasitic Hymenoptera and Tachinidae and candidates for conservation biological control were examined for their attractiveness to Lepidoptera, ...
... Glassy-winged sharpshooter (GWSS), Homalodisca vitripennis (Germar), is a vector of the xylem-inhabitant bacterium Xylella fastidiosa Wells et al., which causes Pierce’s disease of grapevines. Current GWSS control strategies in California, USA include area-wide insecticide applications and mass release of mymarid egg parasitoids, including Gonatocerus ashmeadi Girault. Gas chromatography–mass spec ...
... Augmentative on-farm delivery methods for the parasitoid Habrobracon hebetor (Say) (Hymenoptera: Braconidae) to control the millet head miner (MHM) Heliocheilus albipunctella (de Joannis) (Lepidoptera: Noctuidae) were investigated in Burkina Faso from 2011 to 2012 and in Niger in 2012. Our findings indicate that 7 cm × 10 cm jute bags containing 50 g of millet grains, 30 g of millet flour, 25 Corc ...
... Nanosilica and Pseudomonas sp. was treated in soil to enhance the biocontrol activity against pathogens in maize. The leaf hardness shows an increase in surface roughness and elevated phenols while treating nanobiocomposites. Higher phenols and hardness induce silica accumulation in leaves and initiate more effective physical barriers for crop protection. ...
... Old World climbing fern, Lygodium microphyllum (Cav.) R. Br.; Schizaeles: Lygodiaceae), is among the most problematic invasive weeds in southern Florida, USA, where it smothers and displaces native vegetation. Chemical and mechanical control methods that target aerial fronds may not provide adequate control of L. microphyllum as underground rhizomes produce new fronds following treatment. Alternat ...
... In recent years, Bacillus species have received considerable attention for the biological control of many fungal diseases. In this study, Bacillus amyloliquefaciens Q‐426 was tested for its potential use against a variety of plant pathogens. Our screen for genes involved in the biosynthesis of antifungal agents revealed that the fen and bmy gene clusters are present in the Q‐426 genome. Lipopeptid ...
... The control of Glomerella leaf spot (GLS) in Brazil is solely based on fungicide sprays and new alternatives are needed. In apple, few biological control methods have been evaluated, and most have focused on post-harvest pathogens. Therefore, the objectives of this work were to study the mode of action of three bacterial strains and the commercial product Serenade® (Bacillus subtilis) against the ...
... To formulate an efficient and eco-friendly strategy for the management of cotton seedling disease complex, pot experiments were conducted and the efficiency of eight Bacillus strains against seven fungi involved in the disease were determined. A greenhouse evaluation of the interaction between fungal isolates and Bacillus strains was carried out. The evaluation revealed a very highly significant B ...
... Insect bacterial symbionts contribute to many essential biological functions of their hosts and can also influence host fecundity and fitness. The physiological contribution symbionts provide can aid in immune response and xenobiotic detoxification. Both of these immune factors can directly impact strategies aimed at managing insect populations. One biological control strategy that shows promise i ...
... The green shield bug, Palomena prasina (Hemiptera: Pentatomidae), is a pest of deciduous trees and shrubs throughout Turkey and is found in a large variety of habitats, including gardens. We investigated the facultative cultivable bacterial flora of 10 healthy and 5 dead green shield bugs, collected from the vicinity of Trabzon, Turkey, and tested them for insecticidal activity. Based on the conve ...
Salmonella Enteritidis; bacteriophages; biological control; biological control agents; salmon
Abstract:
... The use of bacteriophages for the biocontrol of food-borne pathogens is increasingly gaining acceptance. In this study, the effectiveness of bacteriophages to reduce Salmonella Enteritidis counts was evaluated in raw and smoked salmon tissues. Groups of 25 samples each were contaminated with S. Enteritidis, treated with a phage mix and then incubated for ten days at 18°C and 4°C. A significant bac ...
Erwinia amylovora; apples; bacteriophages; bioassays; biological control; chemical control; host range; host specificity; leaves; pears; Serbia
Abstract:
... In search for an alternative to chemical control of fire blight, we isolated seven bacteriophages specific to E. amylovora. Three phages were isolated from water, three from symptomless pear leaves and one from apple leaves with characteristic fire blight symptoms. Host-range studies showed that the phages differed in the ability to lyse 40 strains of E. amylovora isolated in Serbia. Based on its ...
... Interactions between invasive pest species, especially those mediated by microbial associates, are poorly understood. In this study, we examined the consequences for the woodwasp, Sirex noctilio, biocontrol program of the bark beetle Ips grandicollis, and its fungal associate, Ophiostoma ips. Pine trees treated with herbicide are used internationally to attract ovipositing S. noctilio and to intro ...
... Sustainable rice production is critical to food security especially in Asia. Effective biocontrol of major rice pests such as the White-Backed Planthopper (Sogatella furcifera, Horváth; WBP) is, hence, of eminent importance. We use newly compiled data from Thailand on the Wrinkle-Lipped Bat (Tadarida plicata, Buchanan), WBP distributions and an iterative modelling approach to quantify the importan ...
... SNF1/AMPK protein kinase plays important roles in fungal development and activation of catabolite-repressed genes. In this study, we characterized the role of the Beauveria bassiana SNF1 ortholog. The vegetative growth of the ΔBbSNF1 mutant was reduced by 16 to 50 % on diverse carbon/nitrogen sources. Transcriptional analysis revealed a collection of proteases and chitinases that were not induced ...
... Ssk1-type response regulator proteins are the core elements of histidine-to-aspartate systems that mediate fungal stress tolerance, a determinant to the biocontrol potential of fungal entomopathogens. We characterized the functions of Beauveria bassiana Ssk1 (Bbssk1) by analyzing multi-phenotypic changes in ΔBbssk1 and differentially expressed genes in the digital gene expression (DGE) libraries o ...
Bacillus thuringiensis; Nosema pyrausta; Ostrinia nubilalis; biological control; corn; males; mating frequency; models; moths; pests; population density; reproduction; risk; sex ratio; surveys; traps; Midwestern United States
Abstract:
... The widespread adoption of transgenic Bt maize in the Midwestern United States has led to historically low populations of the European corn borer, Ostrinia nubilalis (Hübner). Reduced population densities might influence mating and/or disease dynamics in O. nubilalis, potentially amplifying or diminishing the benefits of areawide suppression. We hypothesized that O. nubilalis mating success and in ...
... Of the different integrated seed treatment options tested for the management of Alternaria blight of sunflower, highest benefit was obtained in the seed treatment with Carbendazim+Iprodione (Quintal) at 0.3% in water along with hexaconazole foliar spray (0.1%) followed by seed treatment with Pseudomonas fluorescens (0.8%) in jelly+hexaconazole foliar spray. Efficacy of Pseudomonas fluorescens as s ...
... Trichoderma fungal species are universal soil residents that are also isolated from decaying wood, vegetables, infected mushroom and immunocompromised patients. Trichoderma species usually biosynthesize a plethora of secondary metabolites. In an attempt to explore endophytic fungi from healthy foliar tissues of the plant family Cuppressaceae, we explored Cupressus arizonica, C. sempervirens var. c ...
... In the field experiment conducted for the biomanagement of citrus nematode Tylenchulus semipenetrans on 9 year old citrus trees, observations were made for the induction of defence enzymes activity. The plant samples were collected at 7, 15 and 45ᵗʰ day after the inoculation (DAI) of test bioagents viz., Pseudomonas fluorescens, Trichoderma viride, T. harzianum and Pochonia chlamydosporia and assa ...
... We determined the virulence of Beauveria bassiana , Metarhizium anisopliae , Isaria fumosoroseus and Lecanicilium lecanii against larvae of Chilo suppressalis Walker by bioassay and evaluated several enzymatic and non-enzymatic components. LC ₅₀ values of the entomopathogenic fungi revealed 90, 32, 45,000, 4600, 42,000 and 1,540,000 spores/larva for isolates BB1–BB3 of B. bassiana , I. fumosoroseu ...
... The biocontrol potential of the pteromalid cosmopolitan ectoparasitoid Dinarmus basalis Rondani was investigated on pulse beetle Callosobruchus chinensis (L.), a major pest of stored chickpea. The parasitoid could easily recognize and parasitize the late and larger immature stages of hosts. The order of preference was fourth instar larva
Cucumis sativus; DNA fingerprinting; DNA probes; Pseudomonas; biological control; cucumbers; nucleic acid hybridization; phylogeny; random amplified polymorphic DNA technique; rhizosphere; ribosomal DNA; roots; screening
Abstract:
... A DNA probe specific for biocontrol strains of Pseudomonas was produced by screening randomly amplified polymorphic DNA (RAPD) PCR fragments. Specificity of the probe was assessed by dot blot and colony hybridization. It was used to specifically determine the population of these strains on roots of Cucumis sativus cv. Delikatess. Two polymorphic RAPD fragments of 750 bp, and 550 bp showed identica ...
... Mechanisms of action and effectiveness of the antifungal yeast Cryptococcus laurentii [(Kuff.) C.E. Skinner] strain L5D, were examined against the causal agent of anthracnose Colletotrichum gloeosporioides ((Penz.) Penz. & Sacc.) in mango (Mangifera indica L.). C. laurentii showed a high antagonistic potential in vivo, with significant inhibition of anthracnose (75.88%). Different mechanisms of ac ...
... Previously, we selected Pseudomonas corrugata strains CCR04 and CCR80 as rhizobacteria suppressive to Phytophthora blight of pepper caused by Phytophthora capsici. In this study, we investigated soil microbial activity in pepper plants root-drenched with strains CCR04 and CCR80 in relation to their biocontrol activity, root colonization by using bacterial population counts and scanning electron mi ...
... The yeast-like fungus Aureobasidium pullulans PL5 is a microbial antagonist against postharvest pathogens of fruits. The strain is able to produce hydrolases, including glucanases, chitinases and proteases. The alkaline serine protease gene ALP5 from A. pullulans was cloned, inserted into the vector pPIC9 to construct pPIC9/ALP5, and then expressed in Pichia pastoris strain KM71. ALP5 had a molecu ...
... Aspergillus spp. infection of grape may lead to ochratoxin A (OTA) contamination in processed beverages such as wine and grape juice. The aim of the current study was to evaluate the biocontrol potential of two non-fermenting (Cyberlindnera jadinii 273 and Candida friedrichii 778) and two low-fermenting (Candida intermedia 235 and Lachancea thermotolerans 751) yeast strains against the pathogenic ...
... Herbivore damage often deters pollinator visitation and many invasive plants in North America are pollinator-dependent. This has important implications for the biological control of invasive plants because it means that agents that deter pollinators may have a larger than expected impact on the plant. Yet interactions between pollinators and biocontrol agents are rarely evaluated. Centaurea solsti ...
... Bacillus thuringiensis (Bt) is a microbial pesticide widely used to control crop pests. Its strains have good biocontrol activity against crop insect pest, but lack some desirable characteristics that are found in Bacillus subtilis . An attempt has been made to combine those desirable characteristics; we used a highly effective biocontrol strain of B. thuringiensis in protoplast fusions with a str ...
... For field application of a bacterial strain used to control Phythophthora capsici, we will need a biologically and economically efficient carrier medium. The known antagonist Paenibacillus ehimensisKWN38 was grown in a grass medium where it showed high antifungal and lytic enzyme activities. To demonstrate the potential of P. ehimensisKWN38 for biocontrol of late blight disease in pepper, pot tria ...
... Bacillus sp. RS 5 and Rhizobium sp. RS 12 obtained from rhizosphere of Cicer arietinum were potent glucanase and chitinase producing bacterial species. In dual culture method both the strains inhibited the growth of Fusarium oxysporum, Macrophomina phaseolina, Sclerotinia sclerotiorum and in pot experiment both of them resulted in the increase in germination index. ...
... Thirty-four endophytic actinomycetes were isolated from the roots of native plants of the Algerian Sahara. Morphological and chemical studies showed that twenty-nine isolates belonged to the Streptomyces genus and five were non-Streptomyces. All isolates were screened for their in vitro antifungal activity against Rhizoctonia solani. The six that had the greatest pathogen inhibitory capacities wer ...
... Nine fungal isolates [ Clonostachys rosea (1), Coniothyrium minitans (1), Trichoderma crassum (1), T. hamatum (4), T. rossicum (1) and T. virens (1)] were tested in two bioassays for their ability to degrade sclerotia and reduce apothecial production and carpogenic infection of cabbage seedlings. C. minitans LU112 reduced apothecial production in both bioassays, with T. virens LU556 significantly ...
... The two-spotted spider mite Tetranychus urticae is an important pest on tomato (Solanum lycopersicum) in greenhouses. The predatory mite Phytoseiulus persimilis is often used to control T. urticae, but is not well adapted to tomato plants and does not control T. urticae efficiently on this crop. New predators such as Phytoseiulus macropilis and new active ingredients such as bifenazat (Acramite®) ...
... Seeking new yeast strains having the ability to protect apple fruits against blue mould for a long time under different storage conditions was the main goal of this work. Based on the in vitro test, yeast strains KKUY0017 and KKUY0051 were selected as the most effective antagonists against Penicillium expansum . Sequencing of 26S rDNA of both yeasts confirmed that the identity of KKUY0017 and KKUY ...
... In this work, Metschnikowia pulcherrima Disva 267, Wickerhamomyces anomalus Disva 2, and Saccharomyces cerevisiae Disva 599 yeast strains were evaluated for their biocontrol activity on postharvest decay brown rot, mainly caused by Monilinia laxa on sweet cherries, using three increasing concentrations (106, 107 and 108CFU/mL). M. pulcherrima significantly reduced brown rot incidence, severity and ...
... Gnomonia fragariae Kleb. causes severe root rot and petiole blight of strawberry. The pathogen has been found to be involved in the strawberry root rot complex in Latvia and Sweden. Since the disease has been recently discovered, currently no control measures are available. The biocontrol ability of two non-pathogenic Fusarium sp. strains, A37 and A38, originated from strawberry roots against Gnom ...
... The potential of the fungal antagonist Microsphaeropsis ochracea to control the soilborne pathogen Verticillium longisporum was investigated in environments with varying microbial complexity (in vitro vs. in vivo, sterile vs. unsterile, controlled conditions vs. field). A semi-quantitative PCR assay was developed for the detection of M. ochracea on unsterile plant debris. In vitro, M. ochracea cau ...
... Radula marginata and Cannabis sativa L. are two phylogenetically unrelated plant species containing structurally similar secondary metabolites like cannabinoids. The major objective of our work was the isolation, identification, biocontrol efficacies, biofilm forming potential and anti-biofilm ability of endophytic microbial community of the liverwort R. marginata, as compared to bacterial endophy ...
... Housefly (Musca domestica L.), one of the most common insects in human settlements, has been associated as vectors for various food-borne pathogens, causing food spoilage and disease transmission. The control of housefly was attempted using plant monoterpenes; menthone, menthol, menthyl acetate, limonene, citral and 1,8-cineole, against different life stages of housefly. Bioefficacy against housef ...
... The tea (Camellia sinensis (L.) Kuntze) industry is the backbone of the agroeconomy of the North Bengal region located at the foothills of the Himalayas in North-East (NE) India. This region accounts for about 25% of the total tea production in India. The tea mosquito bug, Helopeltis theivora Waterhouse, is one of the most devastating sucking pests of tea in this region. Various kinds of synthetic ...
... We have isolated three novel strains of Trichoderma (two T. harzianum and one T. atroviride) from wild mushroom and tree bark, and evaluated their biocontrol potential against Sclerotium delphinii infecting cultivated cotton seedlings. T. harzianum strain CICR-G, isolated as a natural mycoparasite on a tree-pathogenic Ganoderma sp. exhibited the highest disease suppression ability. This isolate wa ...
Uejio Christopher K.; Hayden Mary H.; Zielinski-Gutierrez Emily; Lopez Jose Luis Robles; Barrera Roberto; Amador Manuel; Thompson Gregory; Waterman Stephen H.
... Dengue periodically circulates in southern Texas and neighboring Tamaulipas, Mexico; thus, a closer examination of human and vector ecology at the northern limits of North American transmission may improve prevention activities. Scrap tires produce large mosquito populations and increase the risk of dengue transmission. Some households choose not to pay tire disposal fees, and many tires are illeg ...
Diorhabda carinulata; Dipodomys; Tamarix; biological control; biological control agents; defoliation; desertification; habitats; mark-recapture studies; population size; rodents; small mammals; species diversity; trapping; Western United States
Abstract:
... The spread of introduced saltcedar (Tamarix spp.) throughout many riparian systems across the western United States motivated the introduction of saltcedar leaf beetles (Diorhabda carinulata, D. elongata; Chrysomelidae) as a biological control agent specific to saltcedar. I monitored small mammal populations for up to 12 years as saltcedar defoliation progressed at 3 of the original saltcedar beet ...
... Pseudomonas fluorescens HC1-07, previously isolated from the phyllosphere of wheat grown in Hebei province, China, suppresses the soilborne disease of wheat take-all, caused by Gaeumannomyces graminis var. tritici. We report here that strain HC1-07 also suppresses Rhizoctonia root rot of wheat caused by Rhizoctonia solani AG-8. Strain HC1-07 produced a cyclic lipopeptide (CLP) with a molecular wei ...
... In greenhouse and field experiments, a mycelial formulation of the fungus Myrothecium verrucaria (IMI 361690) containing 0.20% Silwet L-77 surfactant exhibited high bioherbicidal efficacy against the problematic weed hemp sesbania. Infection and mortality levels of 100% of hemp sesbania seedlings occurred within 48 h after fungal application in the greenhouse. In rice field tests conducted over a ...
... Colletotrichum truncatum, grown on rice grain (3 to 4 weeks, 22˚C to 24˚C) produced a fungus-infested rice mixture of microsclerotia and conidia (spores) in a ratio of ~9:1, respectively. Greenhouse tests of this formulation (0.4 to 50 mg finely-ground fungus-rice product) which applied pre-emergence to 5 cm2 of soil surface, caused 22% to 96% hemp sesbania plant mortality, after 14 days. Post-eme ...
Rhodobium porosum; biological control; chemical treatment; crops; greenhouses; integrated pest management; parasitoids; soilless culture; strawberries; Belgium; France; Switzerland
Abstract:
... A cocktail of six species of parasitoids has been used to control aphids on strawberry in Switzerland and France in protected and soilless crops. The cocktail was developed by Viridaxis in Belgium and labeled FresaProtect. In Switzerland, this cocktail was tested during two years under tunnel on everbearing strawberries. The results after the first year proved the efficacy and vitality of FresaPro ...
refuge habitats; agroecosystems; parasitism; selection pressure; parthenogenesis; pastures; biological control agents; parasitic wasps; natural enemies; sexual reproduction; invasive species; biodiversity; insects; biological control; New Zealand
Abstract:
... Although records show that the loss of susceptibility of pests to biological control agents is an exceedingly rare event, there are certain behavioural and ecological settings that may well predispose to it. In general, these circumstances rarely converge. Such a critical combination of factors could possibly occur in agroecosystems based on incomplete transplants imported from elsewhere. It can b ...
... D-pinitol is an effective agent for controlling powdery mildew (Podosphaera xanthii) in cucumber. In this study, we determined the mechanisms of D-pinitol in controlling powdery mildew in cucumber plants. We compared P. xanthii development on cucumber leaf surface treated with D-pinitol or water (2 mg ml−1) at different time points after inoculation. The germinating conidia, hyphae, and conidiopho ...
Botrytis cinerea; Candida sake; biological control; biological control agents; climatic factors; field experimentation; fruits; grapes; growing season; laboratory experimentation; relative humidity; temperature; vineyards; virulence; yeasts; France
Abstract:
... Candida sake CPA-1 is an antagonistic yeast that has previously been shown to effectively control Botrytis bunch rot in grapes. The efficacy of biological control agents is dependent on their survival, which may also depend on climatic conditions. However, few studies have evaluated the effect of abiotic factors affecting the survival of biological control agents, such as temperature (T) or relati ...
... Biological control of plant pathogens using benign or beneficial microorganisms as antagonistic agents is currently considered to be an important component of integrated pest management in agricultural crops. In this study, we evaluated the potential of Bacillus subtilis strain HK-CSM-1 as a biological control agent against Colletotrichum panacicola.The potential of B. subtilis HK-CSM-1 as a biolo ...
... One of the most common diseases of tomato (Solanum lycopersicum) is soil-borne Fusarium wilt (Fusarium oxysporum f. sp. lycopersici), which causes serious economic and yield losses. Major strategies for controlling this disease are soil fumigation with methyl bromide, using resistant cultivars, grafting on to resistant rootstocks, and biological control methods. In the present study, the role of m ...