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... Sea buckthorn is a berry crop with multiple uses. The berries are highly appreciated for their unique taste but are also very rich in bioactive compounds with powerful nutritional and medicinal values. In addition, the plants grow well under adverse conditions, and are often used to fight soil erosion. Utilization of sea buckthorn has therefore increased around the world but serious problems have, ...
Arabidopsis thaliana; alleles; death; fungi; gametophytes; genetic analysis; microarray technology; mutants; penetrance; pollen; proteins; reverse genetics; scanning electron microscopy; sequence analysis; transfer DNA
Abstract:
... Arabidopsis thaliana has proven a powerful system for developmental genetics, but identification of gametophytic genes with developmental mutants can be complicated by factors such as gametophyte-lethality, functional redundancy, or poor penetrance. These issues are exemplified by the Plant Intracellular Ras-group LRR (PIRL) genes, a family of nine genes encoding a class of leucine-rich repeat pro ...
... The vascular system in plants, which comprises xylem, phloem and vascular stem cells, originates from provascular cells and forms a continuous network throughout the plant body. Although various aspects of vascular development have been extensively studied, the early process of vascular development remains largely unknown. LONESOME HIGHWAY (LHW), which encodes an atypical basic helix–loop–helix (b ...
... Simple sequence repeats (SSRs) have become one of the most popular molecular markers for population genetic studies. The application of SSR markers has often been limited to source species because SSR loci are too labile to be maintained in even closely related species. However, a few extremely conserved SSR loci have been reported. Here, we tested for the presence of conserved SSR loci in acantho ...
... In Forensic Entomology the fast and accurate identification of insects collected at the scene of events is essential if errors are to be avoided in estimating infestation times of interest and determining the post-mortem interval (PMI). Traditional identification based on morphological characteristics can be complicated due to physical similarities between different species, especially at immature ...
... DNA sequences of cytochrome c oxidase I gene (COI) from Lepidion spp. were employed to test the efficiency of species identification. A sample of 32 individuals from five Lepidion species was sequenced and combined with 26 sequences from other BOLD projects. As a result, 58 Lepidion DNA sequences of the COI gene belonging to eight of the nine recognized Lepidion species were analysed. Sequences we ...
... Dwarfing germplasms are crucial to the development of wheat (Triticum aestivum L.) varieties with ideal plant height and combinations of agronomic traits. A novel dwarfing wheat line, AS34, was developed in this study by somatic variation. This new wheat germplasm was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis, two-dimensional gel electrophoresis, genomic in situ hybrid ...
... Among the known functions of the P II protein (the glnB gene product) in the cyanobacterium Synechococcus elongatus , negative regulation of the activity of PipX, a transcriptional co-activator of the NtcA regulon, has been thought to be essential for cell viability, because all the P II-less mutants thus far constructed carry spontaneous mutations in pipX . PipX is thus deduced to be a toxic prot ...
... Plant life history traits like clonal growth and dioecy can complicate endangered species conservation because of the potential for remnant populations to contain few genetically different individuals or a single sex. In this study, we used 11 microsatellite (SSR) markers to characterize fine-scale genetic structure of the endangered dioecious perennial Lindera melissifolia (pondberry) growing in ...
... Grain shape–related traits are important components determining rice (Oryza sativa L.) grain yield, and they are controlled by quantitative trait loci (QTL). To study the genetic basis of grain shape–related traits, we performed an experiment using a population of rice introgression lines (ILs) in two different environments. The results revealed that the 10 traits investigated were affected by 84 ...
... For development of RILs of cross CSR10 x Taraori Basmati F₉ and F₁₀ generations were studied and analyzed for genetic variability, heritability, correlation, path coefficient analysis, transgressive segregation and regression analysis for five agronomic and seven grain quality traits for which, 208 RILs of F₉ and F₁₀ generations were used. Analysis of variance showed significant variation for all ...
... The genetic organization of the 24 duck circovirus (DuCV) strains detected in commercial Pekin ducks from South Korea between 2011 and 2012 is described in this study. Multiple sequence alignment and phylogenetic analyses were performed on the 24 viral genome sequences as well as on 45 genome sequences available from the GenBank database. Phylogenetic analyses based on the genomic and open reading ...
children; counseling; death; forensic sciences; genetic analysis; infants; ion channels; major genes; mutation; necropsy; risk; screening; tachycardia; young adults
Abstract:
... Each year infants, children and young adults die suddenly and unexpectedly. In many cases the cause of death can be elucidated by medico-legal autopsy, however, a significant number of these cases remain unexplained despite a detailed postmortem investigation and are labeled as sudden unexplained death (SUD). Post-mortem genetic testing, so called molecular autopsy, revealed that primary arrhythmo ...
... Morphological and vocal diversity among closely related species reflects a combination of shared ancestry and recent species-specific adaptations. A small group of Australo-Papuan passerines in the core Corvoidea, the whipbirds and wedgebills (Psophodes, Androphobus), provide an opportunity to explore this. Vocally, the Western Whipbird (Psophodes nigrogularis sensu lato) is very similar to the tw ...
... Genetic components of variance for yield and yield contributing traits were carried out by using 8 x 8 diallel mating design excluding reciprocals. Results revealed that both additive and non-additive gene effects were important for the inheritance of characters studied with preponderance of later for all traits, except 1000-grain weight, kernel breath, kernel L/B ratio, content of amylose and alk ...
... Adults of the fish-borne intestinal trematode species Haplorchis taichui were collected from humans in three provinces of Vietnam: Ha Giang, Thanh Hoa and Quang Tri. Genetic analysis revealed three groups of the parasite from clustering dendrograms, correlating with the localities in which they were collected. Measurements of evolutionary divergence over sequence pairs were greater between the dif ...
Emiliania huxleyi; gene flow; genetic analysis; genetic variation; geographical distribution; microsatellite repeats; morphs; phytoplankton; sexual reproduction; water currents; Antarctic region; Australia
Abstract:
... Cook S.S., Jones R.C., Vaillancourt R.E. and Hallegraeff G.M. 2013. Genetic differentiation among Australian and Southern Ocean populations of the ubiquitous coccolithophore Emiliania huxleyi (Haptophyta). Phycologia 52: 368–374. DOI: 10.2216/12–111.1 Phytoplankton species with a cosmopolitan distribution are traditionally expected to show little genetic differentiation given the lack of geographi ...
... Genetic selection for milking speed is feasible. The existence of a correlation structure between milking speed and milk yield, however, necessitates a selection strategy to increase milking speed with no repercussion on genetic merit for milk yield. Residual milking duration (RMD) and residual milking duration including somatic cell score (RMDS), defined as the residuals from a regression model o ...
... The development of genomic resources in non-model taxa is essential for understanding the genetic basis of biological diversity. Although the genomes of many Drosophila species have been sequenced, most of the phenotypic diversity in this genus remains to be explored. To facilitate the genetic analysis of interspecific and intraspecific variation, we have generated new genomic resources for seven ...
... Phenotypic measurements of plants growing in swards are often confounded by a combination of environmental variation and experimental error. Genetic analysis allows plant scientists to decipher genomic regulation control of key traits, informing breeding strategies and enabling marker-aided breeding. Our research objective was to estimate genotypic variation for a range of key aboveground morpholo ...
... Genetic analyses of planktonic foraminifera have unveiled significant levels of cryptic diversity, thus calling into question the usefulness of the morphological species concept for paleoceanographic reconstructions. Here, we present single-specimen combined genetic and morphological analyses performed on living Truncorotalia truncatulinoides collected across the world oceans. A combined morphogen ...
... Ethylene plays essential roles in adaptive growth of rice plants in water-saturating environment; however, ethylene signaling pathway in rice is largely unclear. In this study, we report identification and characterization of ethylene-response mutants based on the specific ethylene-response phenotypes of etiolated rice seedlings, including ethylene-inhibited root growth and ethylene-promoted coleo ...
Yellow leaf curl disease, caused by a member of Begomovirus (Geminiviridae), is one of important diseases of chilli pepper in Indonesia. Exploration of endophytic fungi was initiated in order to find biological control agents for an alternative control strategies of this disease. Isolates of endophytic fungi were collected from chilli pepper growing area in Sleman, Yogyakarta ...
... An integrated slidable and valveless polymerase chain reaction (PCR) and capillary electrophoresis (CE) microdevice has been developed for pathogen detection on a portable genetic analysis microsystem. The proposed microdevice does not need any microvalve or external pneumatic actuators, thereby simplifying the chip fabrication and the chip operation process. The slidable PCR–CE chip consists of t ...
... Heat stress is a severe environmental factor that significantly reduces plant growth and delays development. Heat stress factors (HSFs) are a class of transcription factors that are synthesized rapidly in response to elevations in temperature and are responsible for the transcription of many heat stress-responsive genes including those encoding heat shock proteins (HSPs). There are 21 HSFs in Arab ...
F-box proteins; active ingredients; chemical elements; cyanides; genetic analysis; herbivores; hydrolysis; seed germination; signal transduction; smoke; strigolactones; wildfires
Abstract:
... Two new types of signaling compounds have been discovered in wildfire smoke due to their ability to stimulate seed germination. The first discovered were karrikins, which share some structural similarity with the strigolactone class of plant hormones, and both signal through a common F-box protein. However, karrikins and strigolactones operate through otherwise distinct signaling pathways, each di ...
... Statistical genetic analysis of quantitative traits in large pedigrees is a formidable computational task due to the necessity of taking the nonindependence among relatives into account. With the growing awareness that rare sequence variants may be important in human quantitative variation, heritability and association study designs involving large pedigrees will increase in frequency due to the g ...
... Acoustic and, to a lesser degree, visual signals are the predominant means of signaling in frogs. Nevertheless, certain lineages such as the mantelline frogs from Madagascar use the chemical communication channel as well. Males possess femoral glands on the hind legs, which recently have been shown to contain volatile compounds used in communication as pheromones. Many mantelline species occur in ...
... The objective of this study was to assess the prevalence of renal cysts and other renal abnormalities in purebred Maine Coon cats, and to characterise these through genetic typing. Voluntary pre-breeding screening programmes for polycystic kidney disease (PKD) are offered for this breed throughout Switzerland, Germany and other northern European countries. We performed a retrospective evaluation o ...
Malus sylvestris; apples; calyx; genetic analysis; hybrids; parents; quantitative traits; random amplified polymorphic DNA technique; testa
Abstract:
... A study of the three Malus species (M. domestica, M. sylvestris, and a hybrid species, M. domestica × M. sylvestris, which was named M. ×oxysepala) was carried out based on the morphological and micromorphological features and molecular investigation. Observations performed for 47 quantitative traits showed that this hybrid species exhibits intermediate values between M. domestica and M. sylvestri ...
... Recently, the applications of nanomaterial-assisted polymerase chain reaction (nanoPCR) have received considerable attention. Several potential mechanisms have been proposed, but mainly according to the results of PCR assays under specific conditions and lacking direct and general evidence. The mechanism of nanoPCR has not been elucidated yet. Here, taking gold nanoparticles (AuNPs) as an example, ...
... A large proportion of eukaryotic genomes is transcribed from both positive and negative strands of DNA and thus may generate overlapping sense and antisense transcripts. Some of these so-called natural antisense transcripts (NATs) are possibly co-regulated. When the overlapping sense and antisense transcripts are expressed at the same time in the same cell in response to various developmental and ...
... It is necessary to use algorithms to analyze gene expression data from DNA microarrays, such as in clustering and machine learning. Previously, we developed the knowledge-based fuzzy adaptive resonance theory (KB-FuzzyART), a clustering algorithm suitable for analyzing gene expression data, to find clues for identifying gene networks. Leaf primordia form around the shoot apical meristem (SAM), whi ...
... Leaf growth and development characteristics are important factor for grain yield. In order to genetic analysis of Leaf growth and development characteristics of rice, a linkage map provided from F2 rice mapping population caused Gharib × Khazar and 111 microsatellite markers. Total number leaf, Days to flag leaf, leaf emergence rate, grain weight, grain number, length and width of flag leaf and br ...
... Heading date and photoperiod sensitivity are fundamental traits that determine rice adaptation to a wide range of geographic environments. By quantitative trait locus (QTL) mapping and candidate gene analysis using whole-genome re-sequencing, we found that Oryza sativa Pseudo-Response Regulator37 (OsPRR37; hereafter PRR37) is responsible for the Early heading7-2 (EH7-2)/Heading date2 (Hd2) QTL whi ...
... Sustainable management of sea mullet (Mugil cephalus) fisheries needs to account for recent observations of regional‐scale differentiation. Population genetic analysis is sought to assess the situation of this ecologically and economically important fish species in eastern Australian waters. Here, we report (i) new population genetic markers [single nucleotide polymorphisms (SNPs) and potential mi ...
... Non-lethal sampling methods are of great interest for conservation genetic studies to prevent the death of individuals in populations that are threatened or in decline. With this aim, we tested a non-lethal method of partial antennae removal for DNA sampling in two euglossine bee species: Euglossa cordata and Eulaema nigrita. We validated the survival of the individuals through mark–recapture expe ...
KP Kulkarni; C Vishwakarma; SP Sahoo; JM Lima; M Nath; P Dokku; RN Gacche; T Mohapatra; S Robin; N Sarla; M Seshashayee; AK Singh; K Singh; NK Singh; RP Sharma
... Plant height and flowering time are two of the important traits that affect plant architecture. Efforts were made in this study to characterize morphologically the-EMS-induced dwarf and early flowering mutants of rice variety Nagina22 and to study their mode of inheritance. Nine true breeding mutants generated earlier by EMS treatment were analysed for differences in their phenotypic characteristi ...
Bemisia tabaci; climate; cryptic species; genetic analysis; genetic markers; genetic variation; genotyping; greenhouses; microsatellite repeats; mitochondria; phylogeography; population genetics; population structure; Hawaii
Abstract:
... The Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) cryptic species complex of whitefiies contains two species, MEAM1 and MED, that are highly invasive in supportive climates the world over. In the United States, MEAM1 occurs both in the field and in the greenhouse, but MED is only found in the greenhouse. To make inferences about the population structure of both species, and the origin and re ...
data collection; demography; effective population size; females; genetic analysis; genetic variation; genome; horses; inbreeding coefficient; males; pedigree; population dynamics; sires; United Kingdom; United States
Abstract:
... The American Shire horse breed is currently listed in “critical condition”, the most serious threat category assigned by the American Livestock Breeds Conservancy. Despite concern for the breed's future, however, a thorough genetic analysis of the breed has not been conducted to date. Pedigree data from 85 671 Shire horses born between 1806 and 2011 were used to determine current and historical ge ...
... The public availability of large quantities of gene sequence data provides a valuable resource of the mining of Simple Sequence Repeat (SSR) molecular genetic markers for genetic analysis. These markers are inexpensive, require minimal labour to produce and can frequently be associated with functionally annotated genes. This study presents the characterization of barley EST‐SSRs and the identifica ...
data quality; databases; dogs; forensic sciences; genetic analysis; haplotypes; mitochondrial DNA; mitochondrial genome; probability
Abstract:
... The identification of dog hair through mtDNA analysis has become increasingly important in the last 15 years, as it can provide associative evidence connecting victims and suspects. The evidential value of an mtDNA match between dog hair and its potential donor is determined by the random match probability of the haplotype. This probability is based on the haplotype’s population frequency estimate ...
... Thanks to recent advances in high-throughput genotyping tools and software for genetic analyses, it is now possible to perform QTL mapping and genome-wide association analysis in tetraploid roses and to establish the effect of favorable SNP marker haplotype(s) (“plus-alleles”). The challenge is how to use these tools and technologies for rose breeding in a cost-effective way. At least four points ...
... The aim of this study was to conduct genetic analysis of 219 cold-blooded Sztumski mares, which were enrolled in the genetic resources conservation programme in 2008. The inbreeding level in the mares, their average genetic relationship with the population, and the average coefficient of kinship were analysed, as well as the number and origin of common ancestors (founders of the population) were d ...
... The interaction of legumes with N ₂-fixing bacteria collectively called rhizobia results in root nodule development. The number of nodules formed is tightly restricted through the systemic negative feedback control by the host called autoregulation of nodulation (AON). Here, we report the characterization and gene identification of TOO MUCH LOVE (TML), a root factor that acts during AON in a model ...
Arabidopsis thaliana; biogeochemical cycles; chloroplasts; data collection; fumonisin B1; gene targeting; genes; genetic analysis; pathogens; programmed cell death; proteins; proteome; proteomics; sucrose; transcriptomics; transfer DNA
Abstract:
... Programmed cell death (PCD) is an essential process that functions in plant organ sculpture, tissue differentiation, nutrient recycling, and defense against pathogen attack. A full understanding of the mechanism of PCD in plants is hindered by the limited identification of protein components of the complex signaling circuitry that underpins this important physiological process. Here we have used A ...
alleles; allelic variation; epigenetics; gene expression; genetic analysis; genomics; human diseases; medicine; signal transduction; single nucleotide polymorphism
Abstract:
... Modern genetic analysis has shown that most polymorphisms associated with human disease are non-coding. Much of the functional information contained in the non-coding genome consists of cis-regulatory sequences (CRSs) that are required to respond to signal transduction cues that direct cell specific gene expression. It has been hypothesised that many diseases may be due to polymorphisms within CRS ...
... The green alga Chlamydomonas reinhardtii evolves H2 gas under anaerobic conditions in reactions catalyzed by hydrogenase enzymes. Expression of the HYDA genes and related genes is regulated by environmental conditions including anoxia. To study the pathways of gene regulation, we utilized the reporter gene RSP3, encoding a radial spoke protein required for flagellar motility. Promoter/5′UTR sequen ...
... Brassinosteroids (BRs) are essential regulators of plant architecture. Understanding how BRs control plant height and leaf angle would facilitate development of new plant type varieties by biotechnology. A number of mutants involved in BR biosynthesis have been isolated but many of them lack detailed genetic analysis. Here, we report the isolation and characterization of a severe dwarf mutant, chr ...
... Approaches and tools to differentiate between natural selection and genetic drift as causes of population differentiation are of frequent demand in evolutionary biology. Based on the approach of Ovaskainen et al. (2011), we have developed an R package (driftsel) that can be used to differentiate between stabilizing selection, diversifying selection and random genetic drift as causes of population ...