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2012
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- Author:
- Elvira Fiallo-Olivé; Jesús Navas-Castillo; Enrique Moriones; Yamila Martínez-Zubiaur
- Source:
- Archives of virology 2012 v.157 no.1 pp. 141-146
- ISSN:
- 0304-8608
- Subject:
- Abutilon; DNA; Euphorbia heterophylla; Euphorbia mosaic virus; Phaseolus vulgaris; Sida golden mosaic virus; Sida rhombifolia; Sida yellow vein virus; green beans; host range; islands; nucleotide sequences; nucleotides; phylogeny; surveys; viruses; weeds; Cuba; Florida
- Abstract:
- ... As a result of surveys conducted during the last few years to search for wild reservoirs of begomoviruses in Cuba, we detected a novel bipartite begomovirus, sida yellow mottle virus (SiYMoV), infecting Sida rhombifolia plants. The complete genome sequence was obtained, showing that DNA-A was 2622 nucleotides (nt) in length and that it was most closely related (87.6% nucleotide identity) to DNA-A ...
- DOI:
- 10.1007/s00705-011-1123-8
- PubMed:
- 21964921
- https://doi.org/10.1007/s00705-011-1123-8
- Author:
- Amanda J. Foss; Edward J. Phlips; Mete Yilmaz; Andrew Chapman
- Source:
- Harmful algae 2012 v.16 no. pp. 98-107
- ISSN:
- 1568-9883
- Subject:
- microscopy; biosynthesis; ribosomal RNA; DNA; springs (water); high performance liquid chromatography; Lyngbya; spring; genes; nucleotide sequences; toxins; toxicity; mass spectrometry; shellfish; crops; Ocala National Forest; Florida
- Abstract:
- ... Lyngbya wollei, a commonly observed cyanobacterium in Florida's spring fed systems, is considered a nuisance organism due to its formation of large benthic and floating mats. Standing crops and mats of Lyngbya from two Florida springs, Silver Glen Springs (Ocala National Forest) and Blue Hole Spring (Ichetucknee Springs State Park), were sampled and characterized via microscopy. A near full-length ...
- DOI:
- 10.1016/j.hal.2012.02.004
- https://doi.org/10.1016/j.hal.2012.02.004
3. Comparison of the ribosomal RNA operon from Texas Phoenix decline and lethal yellowing phytoplasmas
- Author:
- K. Ntushelo; N. A. Harrison; M. L. Elliott
- Source:
- European journal of plant pathology 2012 v.133 no.4 pp. 779-782
- ISSN:
- 0929-1873
- Subject:
- Cocos nucifera; DNA; Phytoplasma; Sabal palmetto; cabbage; coconuts; decline; lethal yellowing; nucleotide sequences; operon; polymerase chain reaction; ribosomal RNA; sequence analysis; Florida; Texas
- Abstract:
- ... A comparison was made of the two palm yellows phytoplasmas affecting palms to determine if the entire ribosomal RNA operon portion of the phytoplasma genome, or portions thereof, could account for the observed palm host differences. Polymerase chain reaction (PCR) was used to amplify a 5.0Â kb DNA fragment consisting of the entire ribosomal RNA operon from a subgroup 16SrIV-D phytoplasma that caus ...
- DOI:
- 10.1007/s10658-012-0007-4
- https://doi.org/10.1007/s10658-012-0007-4
- Author:
- Ward N. A.; Robertson C. L.; Chanda A. K.; Schneider R. W.
- Source:
- Phytopathology 2012 v.102 no.8 pp. 749-760
- ISSN:
- 0031-949X
- Subject:
- DNA; Phakopsora pachyrhizi; antagonists; biological control agents; disease severity; leaves; plant pathogenic fungi; polymerase chain reaction; soybean rust; soybeans; urediniospores; Florida; Louisiana
- Abstract:
- ... The fungus Simplicillium lanosoniveum was isolated from soybean leaves infected with Phakopsora pachyrhizi, the soybean rust pathogen, in Louisiana and Florida. The fungus did not grow or become established on leaf surfaces until uredinia erupted, but when soybean rust signs and symptoms were evident, S. lanosoniveum colonized leaves within 3 days and sporulated within 4 days. Development of new u ...
- DOI:
- 10.1094/PHYTO-01-11-0031
- https://doi.org/10.1094/PHYTO-01-11-0031
- Author:
- Elmer W. H.; Marra R. E.
- Source:
- Plant disease 2012 v.96 no.10 pp. 1577
- ISSN:
- 0191-2917
- Subject:
- DNA; Dianthus caryophyllus; Fusarium oxysporum; Sanguinaria canadensis; agar; ambient temperature; conidia; conidiophores; crown rot; discoloration; disease control; flowers; genes; genetic databases; greenhouses; growth retardation; hosts; pathogenicity; petioles; pigmentation; plant pathology; planting; potting mix; rhizomes; seedlings; sodium hypochlorite; soil drenching; tomatoes; wild flowers; Florida; Nova Scotia; Pennsylvania
- Abstract:
- ... Bloodroot (Sanguinaria canadensis L [Papaveraceae]) is a native herbaceous perennial in eastern North America, found from Nova Scotia to Florida. Although it is a common wildflower, rhizomes of double-flowered forms are sold commercially. Rhizomes planted into a wooded area in Guilford, CT produced healthy stems and flowers for a few years and then began to collapse and die in 2008. The same sympt ...
- DOI:
- 10.1094/PDIS-04-12-0375-PDN
- https://doi.org/10.1094/PDIS-04-12-0375-PDN
- Author:
- Strayer A.; Garcia-Maruniak A.; Sun X.; Schubert T.; Sutton B.
- Source:
- Plant disease 2012 v.96 no.11 pp. 1690
- ISSN:
- 0191-2917
- Subject:
- DNA; Gram-negative bacteria; Pseudomonas cichorii; Stevia rebaudiana; agar; amino acid sequences; amino acids; color; databases; dieback; genes; leaf spot; leaves; medicinal properties; necrosis; ribosomal RNA; silicon carbide; soaking; sugars; sweetness; tap water; translation (genetics); Florida
- Abstract:
- ... During a routine nursery inspection in Hernando County, Florida in July 2011, leaf spot symptoms were observed on the popular sugar substitute stevia (Stevia rebaudiana). Spots were roughly circular to irregular in shape, variable in size, and dark brown with a yellow halo. White to cream-colored, circular, convex, gram-negative bacterial colonies were isolated on nutrient agar and identified as P ...
- DOI:
- 10.1094/PDIS-01-12-0002-PDN
- https://doi.org/10.1094/PDIS-01-12-0002-PDN
- Author:
- Young H. M.; Srivastava P.; Paret M. L.; Dankers H.; Wright D. L.; Marois J. J.; Dufault N. S.
- Source:
- Plant disease 2012 v.96 no.10 pp. 1581
- ISSN:
- 0191-2917
- Subject:
- Brassica carinata; DNA; Sclerotinia sclerotiorum; agar; ambient temperature; biofuels; canola; collard greens; cropping systems; cultivars; fungi; genetic databases; greenhouses; humidity; internal transcribed spacers; lactic acid; lodging; mycelium; oils; pathogenicity; planting; plastic bags; polymerase chain reaction; sandy clay soils; sclerotia; sequence analysis; stem rot; stems; Florida; North Dakota
- Abstract:
- ... Brassica carinata A. Braun, commonly referred to as Ethiopian rapeseed, a near relative of collards and mustard, has become the object of increasing interest as an oil crop. It has been reported that B. carinata adapts better and is more productive than B. napus (canola) in adverse conditions, such as clay and sandy soils and under low management cropping systems (1). In late February 2012, sympto ...
- DOI:
- 10.1094/PDIS-05-12-0469-PDN
- https://doi.org/10.1094/PDIS-05-12-0469-PDN
- Author:
- Delaney M. A.; Sikora E. J.; Delaney D. P.; Palm M. E.; Haudenshield J. S.; Hartman G. L.
- Source:
- Plant disease 2012 v.96 no.9 pp. 1374
- ISSN:
- 0191-2917
- Subject:
- Agricultural Research Service; DNA; DNA primers; Desmodium tortuosum; Phakopsora pachyrhizi; fungi; genetic databases; internal transcribed spacers; leaves; monoclonal antibodies; overwintering; pathogens; polymerase chain reaction; ribosomal RNA; soybean rust; soybeans; type collections; urediniospores; weather; Alabama; Florida
- Abstract:
- ... Soybean rust (SBR), caused by the fungus Phakopsora pachyrhizi, was detected on Florida Beggarweed (Desmodium tortuosum) for the first time in Alabama in November, 2009. The pathogen was not observed in 2010 or 2011, probably because of the exceptionally dry, hot weather in the region. The pathogen was observed on multiple mature leaves of plants, evenly distributed through a field at the Wiregras ...
- DOI:
- 10.1094/PDIS-01-12-0015-PDN
- https://doi.org/10.1094/PDIS-01-12-0015-PDN
- Author:
- Kokalis-Burelle N.; Rosskopf E. N.; Holzinger J.
- Source:
- Plant disease 2012 v.96 no.2 pp. 296
- ISSN:
- 0191-2917
- Subject:
- DNA; Malva parviflora; Meloidogyne arenaria; Solanum lycopersicum; cut flowers; egg masses; eggs; esterases; field experimentation; gravid females; greenhouses; growers; hosts; inoculum; methyl bromide; pest control; phenotype; root-knot nematodes; roots; sand; soil; soil fumigation; species identification; tomatoes; weeds; Algeria; California; Egypt; Florida
- Abstract:
- ... During a 2010 field trial for examining alternatives to methyl bromide soil fumigation for the production of field-grown cut flowers, weeds were collected for identification and evaluated for their potential as hosts for plant pathogenic nematodes. In one cut flower field located in Martin County, FL, six cheeseweed mallow (Malva parviflora L.) plants were collected that had root-galling typical o ...
- DOI:
- 10.1094/PDIS-08-11-0647
- https://doi.org/10.1094/PDIS-08-11-0647
- Author:
- Liotta Janice L.; Lee Alice C. Y.; Koompapong Khuanchai N.; Yaros Joseph P.; Prullage Joseph; Ulrich Michael A.; Bowman Dwight D.
- Source:
- Journal of parasitology 2012 v.98 no.5 pp. 1037-1038
- ISSN:
- 0022-3395
- Subject:
- Ancylostoma tubaeforme; DNA; cats; death; eggs; feces; harvesting; larvae; necropsy; puppies; restriction fragment length polymorphism; sequence analysis; specific pathogen-free animals; sugars; Florida
- Abstract:
- ... Because the eggs of Ancylostoma species of dogs and cats are difficult to readily distinguish morphologically, isolation of a certain species often requires the humane death of the source animal or holding an animal after treatment to obtain worms for specific identification or to harvest ex utero eggs. The objective of this study was to obtain an isolate of Ancylostoma braziliense from 1-time, fi ...
- DOI:
- 10.1645/GE-2981.1
- https://doi.org/10.1645/GE-2981.1
- Author:
- Liotta Janice L.; Lee Alice C. Y.; Aksel Sarp; Alkhalife Ibrahim; Cruz-Reyes Alejandro; Youn HeeJeong; Bienhoff Stephen E.; Bowman Dwight D.
- Source:
- Journal of parasitology 2012 v.98 no.5 pp. 1034-1036
- ISSN:
- 0022-3395
- Subject:
- Ancylostoma caninum; Beagle; DNA; adults; death; dogs; eggs; endoscopy; feces; females; kittens; larvae; males; necropsy; restriction fragment length polymorphism; sequence analysis; sugars; Florida
- Abstract:
- ... Isolation of a specific Ancylostoma species typically requires death of the source animal, or holding an animal long enough to collect feces after treatment, for worm recovery and identification. The reason for collecting worms is that the eggs are not easy to distinguish morphologically. In keeping with the 3 Rs of laboratory animal research (reduction, refinement, replacement), the objective of ...
- DOI:
- 10.1645/GE-2938.1
- https://doi.org/10.1645/GE-2938.1
12. Overview of a classical biological control project directed against the red palm mite in Florida
- Author:
- Marjorie A. Hoy
- Source:
- Experimental & applied acarology 2012 v.57 no.3-4 pp. 381-393
- ISSN:
- 0168-8162
- Subject:
- Amblyseius; DNA; Tenuipalpidae; biological control; mites; predators; rearing; type collections; Florida; Mauritius
- Abstract:
- ... Information is provided on the steps involved in classical biological control programs, with an outline of the steps achieved in the classical biological control of the red palm mite, Raoiella indica (Acari: Tenuipalpidae), in Florida. An overview is provided of the results and an accompanying paper by Bowman and Hoy (2012) describes the molecular analyses conducted to characterize phytoseiid pred ...
- DOI:
- 10.1007/s10493-012-9537-x
- PubMed:
- 22456811
- https://doi.org/10.1007/s10493-012-9537-x
13. Prevalence of Ancylostoma braziliense in Cats in Three Northern Counties of Florida, United States
- Author:
- Liotta Janice L.; Koompapong Khuanchai N.; Yaros Joseph P.; Prullage Joseph; Bowman Dwight D.
- Source:
- Journal of parasitology 2012 v.98 no.5 pp. 1032-1033
- ISSN:
- 0022-3395
- Subject:
- Ancylostoma tubaeforme; DNA; cats; eggs; feces; hookworms; polymerase chain reaction; restriction fragment length polymorphism; sugars; Florida
- Abstract:
- ... A convenience sampling of fecal specimens from 40 cats in northern Florida was examined for the presence of Ancylostoma braziliense eggs by using centrifugal sugar flotation and polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). Of the 40 samples, 26 (65%) contained hookworm eggs. DNA from 24 samples was successfully amplified using PCR; using RFLP, 10 samples were id ...
- DOI:
- 10.1645/GE-2930.1
- https://doi.org/10.1645/GE-2930.1
- Author:
- Liotta Janice L.; Youn HeeJeong; Aksel Sarp; Bienhoff Stephen E.; Bowman Dwight D.
- Source:
- Journal of parasitology 2012 v.98 no.5 pp. 1039-1040
- ISSN:
- 0022-3395
- Subject:
- Ancylostoma; DNA; dogs; eggs; feces; hookworms; polymerase chain reaction; restriction fragment length polymorphism; sugars; Florida
- Abstract:
- ... A convenience collection of fecal samples from 148 dogs in northern Florida was examined for the presence of Ancylostoma braziliense eggs by using centrifugal sugar flotation and polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). Of the 148 samples, 64 (43.2%) contained hookworm eggs. DNA from 42 samples was successfully amplified using PCR; using RFLP, 2 samples were ...
- DOI:
- 10.1645/GE-2927.1
- https://doi.org/10.1645/GE-2927.1
- Author:
- Margaret E. Hunter; Antonio A. Mignucci-Giannoni; Kimberly Pause Tucker; Timothy L. King; Robert K. Bonde; Brian A. Gray; Peter M. McGuire
- Source:
- Conservation genetics 2012 v.13 no.6 pp. 1623-1635
- ISSN:
- 1566-0621
- Subject:
- habitat destruction; microsatellite repeats; Trichechus manatus; endangered species; DNA; population size; genetic variation; boats; Florida; Puerto Rico
- Abstract:
- ... The West Indian manatee (Trichechus manatus) populations in Florida (T. m. latirostris) and Puerto Rico (T. m. manatus) are considered distinct subspecies and are listed together as endangered under the United States Endangered Species Act. Sustained management and conservation efforts for the Florida subspecies have led to the suggested reclassification of the species to a threatened or delisted ...
- DOI:
- 10.1007/s10592-012-0414-2
- https://doi.org/10.1007/s10592-012-0414-2
- Author:
- Linnen Catherine R.; Smith David R.
- Source:
- Proceedings of the Entomological Society of Washington 2012 v.114 no.4 pp. 492-500
- ISSN:
- 0013-8797
- Subject:
- DNA; Neodiprion; Pinus clausa; Pinus taeda; larvae; nucleotide sequences; Florida
- Abstract:
- ... — DNA sequence studies revealed two additional species of Neodiprion in eastern United States, and morphological studies support their recognition. One, which is sympatric with and morphologically similar to Neodiprion abbotii (Leach) in southeastern United States, is recognized as Neodiprion fabricii (Leach), revised status. The larvae feed on Pinus taeda L. and P. echinata Miller. The other, des ...
- DOI:
- 10.4289/0013-8797.114.4.492
- https://doi.org/10.4289/0013-8797.114.4.492