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1. Detection of bacterial DNA in bile of cats with lymphocytic cholangitis

Author:
C.M.A. Otte; O. Pérez Gutiérrez; R.P. Favier; J. Rothuizen; L.C. Penning
Source:
Veterinary microbiology 2012 v.156 no.1-2 pp. 217-221
ISSN:
0378-1135
Subject:
DNA; Helicobacter; bacteria; bile; bile ducts; cats; genes; intestines; ribosomal RNA; species diversity
Abstract:
... In this study, we have successfully used molecular methods based on the amplification of the 16S ribosomal RNA gene on feline bile samples to show that bile of cats with LC is not sterile. This is probably due to the fact that the inflammatory process in the biliary tree causes dilatations. As a result, bacteria can easily migrate from the intestines via the common bile duct. The diversity of spec ...
DOI:
10.1016/j.vetmic.2011.10.023
PubMed:
22101090

2. Effects of Dietary Fiber on the Feline Gastrointestinal Metagenome

Author:
Barry Kathleen A.; Middelbos Ingmar S.; Vester Boler Brittany M.; Dowd Scot E.; Suchodolski Jan S.; Henrissat Bernard; Coutinho Pedro M.; White Bryan A.; Fahey George C.; Swanson Kelly S.
Source:
Journal of Proteome Research 2012 v.11 no.12 pp. 5924-5933
ISSN:
1535-3907
Subject:
Bacteroidetes; DNA; Firmicutes; Proteobacteria; adults; amino acid metabolism; amino acids; carbohydrate binding; carnivores; cats; cellulose; cross-over studies; databases; dietary fiber; feces; fructooligosaccharides; gastrointestinal system; genes; glycosidases; intestinal microorganisms; metagenomics; omnivores; pectins; phylogeny; prediction; sequence analysis; transferases
Abstract:
... Four healthy adult cats were used in a crossover design to determine phylogeny and metabolic functional capacity of the cat’s gastrointestinal microbiota using a metagenomic approach. Healthy adult cats (1.7 years old) were fed diets containing 4% cellulose, fructooligosaccharides (FOS), or pectin for 30 d, at which time fresh fecal samples were collected. Fecal DNA samples from each cat consuming ...
DOI:
10.1021/pr3006809

3. Epidemiological Survey of Trypanosoma cruzi Infection in Domestic Owned Cats from the Tropical Southeast of Mexico

Author:
M. Jiménez‐Coello; K.Y. Acosta‐Viana; E. Guzman‐Marin; A. Gomez‐Rios; A. Ortega‐Pacheco
Source:
Zoonoses and public health 2012 v.59 Suppl s2 no. pp. 102-109
ISSN:
1863-1959
Subject:
Chagas disease; DNA; Triatominae; Trypanosoma cruzi; Western blotting; antibodies; blood sampling; body condition; cat diseases; cats; enzyme-linked immunosorbent assay; epidemiological studies; immunoglobulin G; infectious diseases; monitoring; pathogenesis; patients; polymerase chain reaction; public health; risk factors; urban areas; Mexico
Abstract:
... American trypanosomiasis is an infectious disease of importance for public health and caused by the protozoa Trypanosoma cruzi mainly transmitted by triatomine bugs. The precise role of cats in the peridomestic transmission of T. cruzi and the mechanism by which cats become infected remain uncertain. The objective of this work was to determine the prevalence of T. cruzi infection in domestic cats ...
DOI:
10.1111/j.1863-2378.2012.01463.x
PubMed:
22958254

4. Identification of Bartonella henselae in 2 Cats With Pyogranulomatous Myocarditis and Diaphragmatic Myositis

Author:
M. Varanat; J. Broadhurst; K. E. Linder; R. G. Maggi; E. B. Breitschwerdt
Source:
Veterinary pathology v.49 no.4 pp. 608-611
ISSN:
1544-2217
Subject:
Bartonella henselae; DNA; anemia; animal pathology; bacteria; cats; diaphragm; endocarditis; fever; heart; immunohistochemistry; lymphatic diseases; myocarditis; myositis; reproductive disorders; silver; North Carolina
Abstract:
... Most cats infected with Bartonella henselae remain outwardly healthy carriers for years; however, self-limiting fever, transient anemia, neurologic dysfunction, lymphadenopathy, reproductive disorders, aortic valvular endocarditis, and neutrophilic myocarditis have been described in experimentally or naturally infected cats. Two cats in a North Carolina shelter died with pyogranulomatous myocardit ...
DOI:
10.1177/0300985811404709

5. Improved cryopreservation of domestic cat sperm in a chemically defined medium

Author:
M.M. Vick; H.L. Bateman; C.A. Lambo; W.F. Swanson
Source:
Theriogenology 2012 v.78 no.9 pp. 2120-2128
ISSN:
0093-691X
Subject:
DNA; acrosome; adults; blastocyst; cats; cooling; cryopreservation; egg yolk; eggs; freezing; glycerol; in vitro fertilization; males; nitrogen; semen; sperm capacitation; sperm motility; temperature; thawing; vapors
Abstract:
... The objective was to compare a proprietary egg yolk-based cryopreservation medium with a chemically defined soy-based medium, as well as to examine effects of temperature of glycerol addition on sperm parameters and IVF after freezing and thawing of domestic cat sperm. Semen was collected from adult cats (four males and three ejaculates per male), divided in four equal aliquots, and extended in ei ...
DOI:
10.1016/j.theriogenology.2012.08.009
PubMed:
23110955

6. Infectious feline herpesvirus detected in distant bone and tendon following mucosal inoculation of specific pathogen-free cats

Author:
C.L. Swenson; K. Gardner; S.P. Arnoczky
Source:
Veterinary microbiology 2012 v.160 no.3-4 pp. 484-487
ISSN:
0378-1135
Subject:
specific pathogen-free animals; virus transmission; kidney cells; cats; Felid alphaherpesvirus 1; DNA; polymerase chain reaction; surgical transplantation; tendons; tongue; viremia; cytopathogenicity; bones; genes; viruses
Abstract:
... Emerging evidence suggests that cats infected with feline herpesvirus-1 (FHV-1) may experience a brief viremic phase. The objective of this study was to determine whether natural routes of FHV-1 inoculation could result in viremic transmission of infectious virus to connective tissues (cortical bone, tendon). Three specific pathogen-free cats were experimentally inoculated with FHV-1 via a combine ...
DOI:
10.1016/j.vetmic.2012.06.028
PubMed:
22795772

7. Loss of Retinoblastoma Protein, But Not p53, Is Associated With the Presence of Papillomaviral DNA in Feline Viral Plaques, Bowenoid In Situ Carcinomas, and Squamous Cell Carcinomas

Author:
J. S. Munday; D. Aberdein
Source:
Veterinary pathology v.49 no.3 pp. 538-545
ISSN:
1544-2217
Subject:
mechanism of action; humans; immunohistochemistry; cats; DNA; polymerase chain reaction; squamous cell carcinoma; protein depletion; skinning; DNA-directed DNA polymerase; Lambdapapillomavirus 1
Abstract:
... Although papillomaviral (PV) DNA is frequently present in feline cutaneous squamous cell carcinomas (SCCs), a causative association cannot be proven. Oncogenic human PVs cause neoplastic transformation by inhibiting retinoblastoma (pRb) and p53 activity. Therefore, absence of pRb and p53 immunostaining, along with increased p16 immunostaining, indicates a PV cause in some human SCCs. If PVs cause ...
DOI:
10.1177/0300985811419534
PubMed:
21930804

8. Molecular survey of Rickettsia, Ehrlichia, and Anaplasma infection of domestic cats in Japan

Author:
Hiromi Sasaki; Yasuaki Ichikawa; Yoshimi Sakata; Yasuyuki Endo; Kazuo Nishigaki; Kotaro Matsumoto; Hisashi Inokuma
Source:
Ticks and tick-borne diseases 2012 v.3 no.5-6 pp. 308-311
ISSN:
1877-959X
Subject:
Anaplasma bovis; DNA; Ehrlichia; Feline immunodeficiency virus; Rickettsia; blood; cats; nucleotide sequences; quantitative polymerase chain reaction; ribosomal RNA; screening; sequence analysis; surveys; tick-borne diseases; ticks; Japan
Abstract:
... The prevalence of Rickettsia, Ehrlichia, and Anaplasma in 1764 DNA samples extracted from feline peripheral blood from all 47 prefectures in Japan was evaluated by screening real-time PCR, genus-specific PCR, and DNA nucleotide sequencing. The survey revealed that all cats were negative for Rickettsia infection. Two cats were positive for Ehrlichia or Anaplasma based on the screening PCR assay. Nu ...
DOI:
10.1016/j.ttbdis.2012.10.028

9. Occurrence of Bartonella henselae types I and II in Central Italian domestic cats

Author:
Valentina V. Ebani; Fabrizio Bertelloni; Filippo Fratini
Source:
Research in veterinary science 2012 v.93 no.1 pp. 63-66
ISSN:
0034-5288
Subject:
Bartonella henselae; DNA; antibodies; blood; blood sampling; cats; females; fluorescent antibody technique; genes; genotype; males; polymerase chain reaction; ribosomal RNA; serological surveys; Italy
Abstract:
... Serological and molecular surveys were conducted to determine the occurrence of Bartonella henselae in domestic cats in Central Italy. Samples from 234 pet cats were tested for B. henselae antibodies by indirect immunofluorescence with 78 (33.3%) positive. A PCR assay specific for the Bartonella 16S rRNA gene was carried out on DNA samples extracted from blood of the 234 cats; 26 (11.1%) of the se ...
DOI:
10.1016/j.rvsc.2011.07.013
PubMed:
21824634

10. Prevalence of Ancylostoma braziliense in Cats in Three Northern Counties of Florida, United States

Author:
Liotta Janice L.; Koompapong Khuanchai N.; Yaros Joseph P.; Prullage Joseph; Bowman Dwight D.
Source:
Journal of parasitology 2012 v.98 no.5 pp. 1032-1033
ISSN:
0022-3395
Subject:
Ancylostoma tubaeforme; DNA; cats; eggs; feces; hookworms; polymerase chain reaction; restriction fragment length polymorphism; sugars; Florida
Abstract:
... A convenience sampling of fecal specimens from 40 cats in northern Florida was examined for the presence of Ancylostoma braziliense eggs by using centrifugal sugar flotation and polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). Of the 40 samples, 26 (65%) contained hookworm eggs. DNA from 24 samples was successfully amplified using PCR; using RFLP, 10 samples were id ...
DOI:
10.1645/GE-2930.1

11. Prevalence of Toxoplasma gondii, Bartonella species and haemoplasma infection in cats in South Africa

Author:
Remo Lobetti; Michael R Lappin
Source:
Journal of feline medicine and surgery v.14 no.12 pp. 857-862
ISSN:
1532-2750
Subject:
Anaplasma; Bartonella henselae; DNA; Ehrlichia; Mycoplasma haemofelis; Mycoplasma haemominutum; Toxoplasma gondii; antibodies; blood serum; cat diseases; cats; diagnostic techniques; enzyme-linked immunosorbent assay; immunoglobulin G; immunoglobulin M; pathogens; patients; polymerase chain reaction; seroprevalence; South Africa
Abstract:
... Vector-borne agents and Toxoplasma gondii are common in cats, with many being zoonotic. The current study investigated the prevalence of selected infectious agents in cats from Johannesburg, South Africa, for which no published data exists. Whole blood and sera were obtained from 102 cats with a variety of disease conditions. Total DNA was extracted from the blood and assayed using PCR techniques ...
DOI:
10.1177/1098612X12452495

12. Real-time PCR as a surveillance tool for the detection of Trichinella infection in muscle samples from wildlife

Author:
Leigh Cuttell; Sean W. Corley; Christian P. Gray; Paul B. Vanderlinde; Louise A. Jackson; Rebecca J. Traub
Source:
Veterinary parasitology 2012 v.188 no.3-4 pp. 285-293
ISSN:
0304-4017
Subject:
DNA; European Union; Trichinella pseudospiralis; cats; crocodiles; detection limit; diagnostic sensitivity; diagnostic techniques; digestion; foxes; host specificity; hosts; humans; larvae; mice; monitoring; muscles; parasites; polymerase chain reaction; pork; ribosomal RNA; risk; swine; wildlife; zoonoses
Abstract:
... Trichinella nematodes are the causative agent of trichinellosis, a meat-borne zoonosis acquired by consuming undercooked, infected meat. Although most human infections are sourced from the domestic environment, the majority of Trichinella parasites circulate in the natural environment in carnivorous and scavenging wildlife. Surveillance using reliable and accurate diagnostic tools to detect Trichi ...
DOI:
10.1016/j.vetpar.2012.03.054
PubMed:
22541794

13. The molecular detection of relaxin and its receptor RXFP1 in reproductive tissue of Felis catus and Lynx pardinus during pregnancy

Author:
Beate C Braun; Astrid Vargas; Katarina Jewgenow
Source:
Reproduction 2012 v.143 no.3 pp. 399-410
ISSN:
1470-1626
Subject:
DNA; Lynx; amino acid sequences; animal ovaries; blood vessels; cats; gene expression; immunohistochemistry; messenger RNA; peptides; placenta; pregnancy; pregnancy diagnosis; quantitative polymerase chain reaction; relaxin; uterine tissue
Abstract:
... Relaxin acts as a pregnancy-specific signal in feline species, but specific information about protein structure and binding is essential for the improvement of pregnancy diagnosis in endangered feline species, like the Iberian lynx. To generate a felid-specific relaxin antibody, the DNA and protein sequences of lynx and cat were determined and peptides were chosen for antibody generation. In addit ...
DOI:
10.1530/REP-11-0316
PubMed:
22187673

14. Classification of Pasteurella species B as Pasteurella oralis sp. nov.

Author:
Henrik Christensen; Mads F. Bertelsen; Anders Miki Bojesen; Magne Bisgaard
Source:
International journal of systematic and evolutionary microbiology 2012 v.62 no.Pt_6 pp. 1396-1401
ISSN:
1466-5034
Subject:
Atelerix albiventris; DNA; Galago; Pasteurella canis; Pasteurella stomatis; bacteria; cadaverine; cats; dogs; fatty acids; ferrets; genes; gingiva; humans; new species; nucleotide sequences; phylogeny; pneumonia; putrescine; quinones; ribosomal RNA; sequence homology; spermidine; spermine
Abstract:
... Pasteurella species B has so far only been reported from the oral cavity of dogs, cats and a ferret. In the present study, information from 15 recent isolates from different sources, including African hedgehogs (Atelerix albiventris), banded mongoose (Mungos mungo), Moholi bushbabies (Galago moholi) and pneumonia of a cat, were compared to five strains investigated previously from bite wounds in h ...
DOI:
10.1099/ijs.0.035246-0
PubMed:
21841008

15. Cryopreservation of Cat Testicular Tissues: Effects of Storage Temperature, Freezing Protocols and Cryoprotective Agents

Author:
P Thuwanut; K Chatdarong
Source:
Reproduction in domestic animals 2012 v.47 no.5 pp. 777-781
ISSN:
0936-6768
Subject:
DNA; Felidae; acridine orange; cats; cryopreservation; cutting; dimethyl sulfoxide; ethylene glycol; freezing; nitrogen; plasma membrane; spermatozoa; storage temperature; testes; transportation; vapors; vitrification; wild animals
Abstract:
... Cryopreservation of testicular tissue has become a part of gamete preservation in wild animal post‐mortem. Using domestic cats as a model for wild felids, this study aimed to (i) investigate the effect of temperature for testicular tissue storage on sperm quality; (ii) compare efficiency of freezing protocols; and (iii) evaluate properties of cryoprotective agents to protect testicular sperm quali ...
DOI:
10.1111/j.1439-0531.2011.01967.x
PubMed:
22151534

16. Detection of Zoonotic Intestinal Parasites in Public Parks of Spain. Potential Epidemiological Role of Microsporidia

Author:
D. Dado; F. Izquierdo; O. Vera; A. Montoya; M. Mateo; S. Fenoy; A. L. Galván; S. García; A. García; E. Aránguez; L. López; C. del Águila; G. Miró
Source:
Zoonoses and public health 2012 v.59 no.1 pp. 23-28
ISSN:
1863-1959
Subject:
parasites; Entamoeba histolytica; Toxocara; public health; Cryptosporidium; DNA; feces; Microsporidia; geophagia; Strongyloides; parks; cats; dogs; polymerase chain reaction; children; Giardia; soil sampling; larvae; Toxascaris leonina; Trichuris vulpis; Spain
Abstract:
... Several studies have demonstrated that the soil of public parks presents an important source of infection which has a significant impact on public health. Children are the main group affected by accidentally ingestion of contaminated soil. This study was performed in order to identify the presence of zoonotic parasites in dog and cat faecal and soil samples from public parks of Madrid, Spain. Six ...
DOI:
10.1111/j.1863-2378.2011.01411.x
PubMed:
21824364

17. Effects of cold storage on plasma membrane, DNA integrity and fertilizing ability of feline testicular spermatozoa

Author:
Sirirak Buarpung; Theerawat Tharasanit; Pierre Comizzoli; Mongkol Techakumphu
Source:
Animal reproduction science 2012 v.131 no.3-4 pp. 219-227
ISSN:
0378-4320
Subject:
DNA; DNA fragmentation; cats; cold storage; embryogenesis; intracytoplasmic sperm injection; plasma membrane; spermatozoa; storage time
Abstract:
... This study examined the effects of cold storage on plasma membrane, DNA integrity, and fertilizing ability of domestic cat spermatozoa. Intact cat testes were stored at 4°C in Dulbecco's phosphate buffered saline (DPBS) for 7 days. Membrane integrity (experiment 1) and DNA integrity (experiment 2) of extracted spermatozoa were assessed over time during storage. Testicular spermatozoa were also tes ...
DOI:
10.1016/j.anireprosci.2012.03.011

18. Identification of parvovirus in the bone marrow of eight cats

Author:
SM Haynes; SA Holloway
Source:
Australian veterinary journal 2012 v.90 no.4 pp. 136-139
ISSN:
0005-0423
Subject:
Carnivore protoparvovirus 1; cats; DNA; polymerase chain reaction; bone marrow; viruses; adults
Abstract:
... Objective To determine if canine parvovirus (CPV) or feline panleucopenia virus (FPV) genomic sequences are present in adult feline bone marrow samples. Design Bone marrow samples were obtained from 32 semi‐feral cats that were euthanased at an animal shelter. DNA was extracted and subjected to conventional polymerase chain reaction (PCR) designed to determine if CPV or FPV DNA was present. Positi ...
DOI:
10.1111/j.1751-0813.2012.00899.x
PubMed:
22443328

19. Molecular detection and characterization of potentially new Babesia and Theileria species/variants in wild felids from Kenya

Author:
Naftaly Githaka; Satoru Konnai; Edward Kariuki; Esther Kanduma; Shiro Murata; Kazuhiko Ohashi
Source:
Acta tropica 2012 v.124 no.1 pp. 71-78
ISSN:
0001-706X
Subject:
Acinonyx jubatus; Babesia canis; DNA; Panthera pardus; Theileria; babesiosis; carnivores; cats; diet; drugs; erythrocytes; genes; genotype; new species; nutrient deficiencies; parasites; phylogeny; ribosomal RNA; Kenya
Abstract:
... Piroplasms frequently infect domestic and wild carnivores. At present, there is limited information on the occurrence and molecular identity of these tick-borne parasites in wild felids in Kenya. In 2009, a pair of captive lions (Panthare leo) was diagnosed with suspected babesiosis and mineral deficiency at an animal orphanage on the outskirts of Nairobi, Kenya. Blood smears indicated presences o ...
DOI:
10.1016/j.actatropica.2012.06.013

20. Molecular detection of tick-borne bacterial agents in Brazilian and exotic captive carnivores

Author:
M.R. André; J.S. Dumler; D.G. Scorpio; R.H.F. Teixeira; S.M. Allegretti; R.Z. Machado
Source:
Ticks and tick-borne diseases 2012 v.3 no.4 pp. 247-253
ISSN:
1877-959X
Subject:
Anaplasma; Canis lupus; DNA; Ehrlichia; Felidae; Rickettsia; blood; captive animals; carnivores; cats; dogs; foxes; genes; pathogens; polymerase chain reaction; ribosomal RNA; tick-borne diseases; ticks; wolves; zoos; Brazil
Abstract:
... The present study aims to detect and characterize by molecular techniques, the presence of tick-borne pathogens in wild captive carnivore blood samples from Brazil. Blood was collected from 76 Brazilian felids, 23 exotic felids, 3 European wolves (Canis lupus), and 97 Brazilian canids maintained in captivity in zoos located in São Paulo and Mato Grosso states, Brazil. DNA of each sample was used i ...
DOI:
10.1016/j.ttbdis.2012.04.002