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- Author:
- Jeannine M. Coburn; Matthew Gibson; Sean Monagle; Zachary Patterson; Jennifer H. Elisseeff
- Source:
- Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.25 pp. 10012-10017
- ISSN:
- 0027-8424
- Subject:
- cartilage; cell proliferation; chondrogenesis; chondroitin sulfate; collagen; extracellular matrix; gene expression; hydrocolloids; in vitro studies; nanofibers; polyvinyl alcohol; proteoglycans; rats; stems; tissue repair
- Abstract:
- ... Articular cartilage repair remains a significant and growing clinical challenge with the aging population. The native extracellular matrix (ECM) of articular cartilage is a 3D structure composed of proteinaceous fibers and a hydrogel ground substance that together provide the physical and biological cues to instruct cell behavior. Here we present fibrous scaffolds composed of poly(vinyl alcohol) a ...
- DOI:
- 10.1073/pnas.1121605109
- PubMed:
- 22665791
- PubMed Central:
- PMC3382486
- https://doi.org/10.1073/pnas.1121605109
- Author:
- Yung-Chih Kuo; Cheng-Chin Wang
- Source:
- Colloids and surfaces 2012 v.93 no. pp. 235-240
- ISSN:
- 0927-7765
- Subject:
- adhesion; bioactive properties; biocompatible materials; bioreactors; cartilage; cattle; cell viability; chondrocytes; chondrogenesis; collagen; colloids; glycosaminoglycans; medicine; scanning electron microscopes; secretion; tissue engineering
- Abstract:
- ... Formation of neocartilage is a critical issue in contemporary regenerative medicine. This study presents the generation of tissue engineering cartilage in TATVHL peptide-grafted scaffolds. Bovine knee chondrocytes were seeded in TATVHL peptide-grafted scaffolds and cultured in a spinner bioreactor. The results revealed that surface TATVHL peptide enhanced the adhesion of bovine knee chondrocytes i ...
- DOI:
- 10.1016/j.colsurfb.2012.01.012
- https://doi.org/10.1016/j.colsurfb.2012.01.012
- Author:
- Nastaran Mahmoudifar; Pauline M. Doran
- Source:
- Trends in biotechnology 2012 v.30 no.3 pp. 166-176
- ISSN:
- 0167-7799
- Subject:
- cartilage; chondrogenesis; engineers; markets; stem cells; tissue engineering
- Abstract:
- ... Joint injury and disease are painful and debilitating conditions affecting a substantial proportion of the population. The idea that damaged cartilage in articulating joints might be replaced seamlessly with tissue-engineered cartilage is of obvious commercial interest because the market for such treatments is large. Recently, a wealth of new information about the complex biology of chondrogenesis ...
- DOI:
- 10.1016/j.tibtech.2011.09.002
- https://doi.org/10.1016/j.tibtech.2011.09.002
- Author:
- Yung-Chih Kuo; Chiu-Yen Chung
- Source:
- Colloids and surfaces 2012 v.93 no. pp. 85-91
- ISSN:
- 0927-7765
- Subject:
- adhesion; biocompatible materials; bioreactors; cartilage; cattle; chitin; chitosan; chondrocytes; chondrogenesis; collagen; colloids; crosslinking; elastin; freeze drying; glycosaminoglycans; polyethylene glycol; surgery; tissue engineering
- Abstract:
- ... A tissue engineering cartilage is of great importance in the current diarthrodial surgery. This study presents the formation of neocartilage by cultivating chondrocytes in elastin- and poly-l-lysine-modified scaffolds. The hybrid bulk biomaterials used contained polyethylene oxide, chitin, and chitosan and were fabricated by crosslinking, pre-freezing, and lyophilization. Bovine knee chondrocytes ...
- DOI:
- 10.1016/j.colsurfb.2011.12.011
- https://doi.org/10.1016/j.colsurfb.2011.12.011
- Author:
- Tali Re’em; Yael Kaminer-Israeli; Emil Ruvinov; Smadar Cohen
- Source:
- Biomaterials 2012 v.33 no.3 pp. 751-761
- ISSN:
- 0142-9612
- Subject:
- binding capacity; bioactive properties; cartilage; cell differentiation; chondrocytes; chondrogenesis; collagen; humans; mice; signal transduction; stem cells; transforming growth factor beta 1
- Abstract:
- ... Herein we describe a bio-inspired, affinity binding alginate-sulfate scaffold, designed for the presentation and sustained release of transforming growth factor beta 1 (TGF-β1), and examine its effects on the chondrogenesis of human mesenchymal stem cells (hMSCs). When attached to matrix via affinity interactions with alginate sulfate, TGF-β1 loading was significantly greater and its initial relea ...
- DOI:
- 10.1016/j.biomaterials.2011.10.007
- https://doi.org/10.1016/j.biomaterials.2011.10.007
- Author:
- Nandana Bhardwaj; Subhas C. Kundu
- Source:
- Biomaterials 2012 v.33 no.10 pp. 2848-2857
- ISSN:
- 0142-9612
- Subject:
- Antheraea mylitta; Bombyx mori; adults; bone marrow; cartilage; chitosan; chondrogenesis; collagen; confocal microscopy; cultured cells; electrolytes; genes; glycosaminoglycans; immunohistochemistry; mulberries; phenotype; quantitative polymerase chain reaction; rats; scanning electron microscopy; silk; silkworms; stem cells
- Abstract:
- ... Adult bone marrow derived mesenchymal stem cells are undifferentiated, multipotential cells and have the potential to differentiate into multiple lineages like bone, cartilage or fat. In this study, polyelectrolyte complex silk fibroin/chitosan blended porous scaffolds were fabricated and examined for its ability to support in vitro chondrogenesis of mesenchymal stem cells. Silk fibroin matrices p ...
- DOI:
- 10.1016/j.biomaterials.2011.12.028
- https://doi.org/10.1016/j.biomaterials.2011.12.028
- Author:
- Dutmanee Seriwatanachai; Nateetip Krishnamra; Narattaphol Charoenphandhu
- Source:
- Biochemical and biophysical research communications 2012 v.420 no.1 pp. 108-113
- ISSN:
- 0006-291X
- Subject:
- apoptosis; chondrocytes; chondrogenesis; females; gene expression; growth plate; lactation; males; messenger RNA; mice; mineralization; pregnancy; prolactin; proteoglycans; rats; viability
- Abstract:
- ... A recent investigation in lactating rats has provided evidence that the lactogenic hormone prolactin (PRL) increases endochondral bone growth and bone elongation, presumably by accelerating apoptosis of hypertrophic chondrocytes in the growth plate and/or subsequent chondrogenic matrix mineralization. Herein, we demonstrated the direct chondroregulatory action of PRL on proliferation, differentiat ...
- DOI:
- 10.1016/j.bbrc.2012.02.123
- https://doi.org/10.1016/j.bbrc.2012.02.123
- Author:
- Sukarto Abby; Yu Claire; Flynn Lauren E.; Amsden Brian G.
- Source:
- Biomacromolecules 2012 v.13 no.8 pp. 2490-2502
- ISSN:
- 1526-4602
- Subject:
- bone morphogenetic proteins; cartilage; chitosan; chondrogenesis; collagen; glycosaminoglycans; humans; hydrogels; modulus of elasticity; polymers; protein synthesis; reverse transcriptase polymerase chain reaction; stem cells; transforming growth factor beta 3; viability
- Abstract:
- ... The coencapsulation of growth factor-loaded microspheres with adipose-derived stem cells (ASCs) within a hydrogel matrix was studied as a potential means to enhance ASC chondrogenesis in the development of a cell-based therapeutic strategy for the regeneration of partial thickness chondral defects. A photopolymerizable N-methacrylate glycol chitosan (MGC) was employed to form an in situ gel used t ...
- DOI:
- 10.1021/bm300733n
- https://doi.org/10.1021/bm300733n
- Author:
- Su Yeon Jeon; Ji Sun Park; Han Na Yang; Dae Gyun Woo; Keun-Hong Park
- Source:
- Biomaterials 2012 v.33 no.17 pp. 4413-4423
- ISSN:
- 0142-9612
- Subject:
- Western blotting; cell differentiation; chondrogenesis; ethyleneimine; fluorescent antibody technique; gels; gene expression; genes; green fluorescent protein; humans; image analysis; immunohistochemistry; light scattering; nanoparticles; plasmids; polymers; reverse transcriptase polymerase chain reaction; scanning electron microscopes; scanning electron microscopy; small interfering RNA; stem cells; transfection
- Abstract:
- ... Some genes expressed in stem cells interrupt and/or enhance differentiation. Therefore, the aim of this study was to inhibit the expression of unnecessary genes and enhance the expression of specific genes involved in stem cell differentiation by using small interfering RNA (siRNA) and plasmid DNA (pDNA) incorporated into cationic polymers as co-delivery factors. To achieve co-delivery of siRNA an ...
- DOI:
- 10.1016/j.biomaterials.2012.02.051
- https://doi.org/10.1016/j.biomaterials.2012.02.051
- Author:
- A. Heidari; Z. Nariman Saleh Fam; E. Esmaeilzadeh-Gharehdaghi; M. Banan; S. Hosseinkhani; S. Mohammadparast; M. Oladnabi; M.R. Ebrahimpour; M. Soosanabadi; T. Farokhashtiani; H. Darvish; S. Ghasemi Firouzabadi; S. Farashi; H. Najmabadi; M. Ohadi
- Source:
- Gene 2012 v.492 no.1 pp. 195-198
- ISSN:
- 0378-1119
- Subject:
- cell cycle; chondrogenesis; databases; gene expression; genes; haplotypes; humans; messenger RNA; mice; nucleosomes; phenotype; promoter regions; purine nucleotides; transcription (genetics)
- Abstract:
- ... In a genome-scale analysis of the composition of core promoter sequences, we have recently shown that approximately 25% of the human protein-coding genes have at least one short tandem repeat (STR) of 3-repeats in their core promoters (i.e. the interval between −120 to +1). Through their nucleosome processing effect, GA-repeats play a crucial role in the regulation of gene transcription. In this s ...
- DOI:
- 10.1016/j.gene.2011.10.028
- PubMed:
- 22037607
- https://doi.org/10.1016/j.gene.2011.10.028
- Author:
- Eleonora Iacono; Marco Cunto; Daniele Zambelli; Francesca Ricci; Pier Luigi Tazzari; Barbara Merlo
- Source:
- Veterinary research communications 2012 v.36 no.2 pp. 107-118
- ISSN:
- 0165-7380
- Subject:
- alizarin; allantoic fluid; bone formation; carbon dioxide; cats; cell culture; chondrogenesis; cytoplasm; droplets; extraembryonic membranes; flow cytometry; glycosaminoglycans; human diseases; human physiology; humans; lipids; lipogenesis; models; oils; stem cells
- Abstract:
- ... Domestic cats are preferred models for normal physiology and several human diseases. In the present study feline fetal fluids and membranes were evaluated as possible sources of MSCs. Samples were recovered from 4 pregnant queens after ovarian-hysterectomy. Gestational sacs were separated from uterine wall; after allantoic and amniotic fluids aspiration and chorion-allantois and amniotic membranes ...
- DOI:
- 10.1007/s11259-012-9520-3
- PubMed:
- 22327440
- https://doi.org/10.1007/s11259-012-9520-3
- Author:
- Hyuck Joon Kwon; Yoshihiro Ohmiya; Kazunori Yasuda
- Source:
- Analytical biochemistry 2012 v.430 no.1 pp. 45-47
- ISSN:
- 0003-2697
- Subject:
- Coleoptera; adenosine triphosphate; biochemical pathways; calcium; chondrogenesis; energy; energy metabolism; luciferase; monitoring
- Abstract:
- ... Although Ca²⁺ regulates energy metabolism through diverse pathways, there have been no methods to monitor both Ca²⁺ dynamics and metabolic activity simultaneously. Here we report a novel system for simultaneously monitoring intracellular Ca²⁺ and ATP levels using a blue-emitting photoprotein and a red-emitting beetle luciferase. Using this system, we monitored the dynamic changes simultaneously in ...
- DOI:
- 10.1016/j.ab.2012.07.015
- https://doi.org/10.1016/j.ab.2012.07.015
- Author:
- Azadeh Kabiri; Ebrahim Esfandiari; Batool Hashemibeni; Mohammad Kazemi; Mohammad Mardani; Abolghasem Esmaeili
- Source:
- Biochemical and biophysical research communications 2012 v.424 no.2 pp. 234-238
- ISSN:
- 0006-291X
- Subject:
- adipose tissue; adult stem cells; adults; cartilage; cell proliferation; cell transplantation; chondrogenesis; collagen; extracellular matrix; gene expression; growth factors; humans; messenger RNA; patients; phenotype; quantitative polymerase chain reaction; tissue engineering
- Abstract:
- ... Injured cartilage is difficult to repair due to its poor vascularisation. Cell based therapies may serve as tools to more effectively regenerate defective cartilage. Both adult mesenchymal stem cells (MSCs) and human adipose derived stem cells (hADSCs) are regarded as potential stem cell sources able to generate functional cartilage for cell transplantation. Growth factors, in particular the TGF-b ...
- DOI:
- 10.1016/j.bbrc.2012.06.082
- https://doi.org/10.1016/j.bbrc.2012.06.082
- Author:
- Ning Kang; Xia Liu; Yue Guan; Jian Wang; Fuxing Gong; Xun Yang; Li Yan; Qian Wang; Xin Fu; Yilin Cao; Ran Xiao
- Source:
- Biomaterials 2012 v.33 no.18 pp. 4535-4544
- ISSN:
- 0142-9612
- Subject:
- biomechanics; bone formation; cartilage; chondrocytes; chondrogenesis; coculture; gene expression; genes; hypertrophy; modulus of elasticity; quantitative analysis; stem cells
- Abstract:
- ... Co-culture of BMSCs and chondrocytes is considered as a promising strategy to generate tissue engineered cartilage as chondrocytes induce the chondrogenesis of BMSCs and inhibit the hypertrophy of engineered cartilage. Because the tissue specific stem/progenitor cells have been isolated from mature tissues including auricular cartilage, we hypothesized that adding stem cells to auricular chondrocy ...
- DOI:
- 10.1016/j.biomaterials.2012.03.019
- https://doi.org/10.1016/j.biomaterials.2012.03.019
- Author:
- Qi Liu; Qilong Wan; Rongtao Yang; Haihua Zhou; Zubing Li
- Source:
- Biochemical and biophysical research communications 2012 v.424 no.1 pp. 182-188
- ISSN:
- 0006-291X
- Subject:
- alkaline phosphatase; bone formation; bone morphogenetic proteins; chondrocytes; chondrogenesis; collagen; gene expression; gene expression regulation; growth factors; messenger RNA; osteocalcin; parathyroid hormone; protein synthesis; somatotropin; transcription factors
- Abstract:
- ... Endochondral ossification is a complex process involving chondrogenesis and osteogenesis regulated by many hormones and growth factors. Parathyroid hormone (PTH), one of the key hormones regulating bone metabolism, promotes osteoblast differentiation and osteogenesis by intermittent administration, whereas continuous PTH administration inhibits bone formation. However, the effects of PTH on chondr ...
- DOI:
- 10.1016/j.bbrc.2012.06.106
- https://doi.org/10.1016/j.bbrc.2012.06.106
- Author:
- Karsten Schrobback; Travis Jacob Klein; Ross Crawford; Zee Upton; Jos Malda; David Ian Leavesley
- Source:
- Cell and tissue research 2012 v.347 no.3 pp. 649-663
- ISSN:
- 0302-766X
- Subject:
- bioreactors; cartilage; cell culture; cell differentiation; cell proliferation; chondrocytes; chondrogenesis; collagen; flow cytometry; gene expression regulation; genes; humans; hypoxia; messenger RNA; normoxia; oxygen; phenotype; plastics; proteoglycans; tissue culture
- Abstract:
- ... Regenerative medicine-based approaches for the repair of damaged cartilage rely on the ability to propagate cells while promoting their chondrogenic potential. Thus, conditions for cell expansion should be optimized through careful environmental control. Appropriate oxygen tension and cell expansion substrates and controllable bioreactor systems are probably critical for expansion and subsequent t ...
- DOI:
- 10.1007/s00441-011-1193-7
- PubMed:
- 21638206
- https://doi.org/10.1007/s00441-011-1193-7
- Author:
- Malihezaman Monsefi; Fatemeh Parvin; Tahereh Talaei-Khozani
- Source:
- British journal of nutrition 2012 v.107 no.5 pp. 683-690
- ISSN:
- 1475-2662
- Subject:
- alizarin; bone formation; bone resorption; calcium; cartilage; cell culture; cell proliferation; cell viability; chondrogenesis; dietary supplements; femur; fetus; fruit extracts; juices; mice; polyphenols; pomegranates; pregnancy; tibia
- Abstract:
- ... Pomegranate is a rich source of polyphenols, which are believed to be responsible for the oestrogenic activities of extracts of this fruit in mice. One of these potential activities is the prevention of bone loss. The objectives of the present study were to determine the effects of pomegranate extract on chondrogenesis and osteogenesis in mouse embryos in vivo and limb bud cultures in vitro. A tot ...
- DOI:
- 10.1017/S0007114511003394
- PubMed:
- 21781378
- https://doi.org/10.1017/S0007114511003394
- Author:
- Bård Spachmo; Augustine Arukwe
- Source:
- Aquatic toxicology 2012 v.108 no. pp. 112-124
- ISSN:
- 0166-445X
- Subject:
- Salmo salar; bone formation; carboxylic acids; chondrogenesis; chronic exposure; eggs; gene expression; genes; head; insulin-like growth factor I; larvae; larval development; polymerase chain reaction; skeletal development; somatotropin; steroid hormones; tanks; thyrotropin
- Abstract:
- ... In this study, we have investigated the effect of perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) on endocrine signalling, growth and development in Atlantic salmon (Salmo salar) embryos and larvae. Expression of genes related to the hypothalamic–pituitary–thyroid (HPT) axis, growth-hormone/insulin-like growth factor (GH/IGF) axis and the steroid hormone axis were used as indica ...
- DOI:
- 10.1016/j.aquatox.2011.07.018
- PubMed:
- 22265611
- https://doi.org/10.1016/j.aquatox.2011.07.018
- Author:
- Masahiko Takahata; Hani A. Awad; Regis J. O’Keefe; Susan V. Bukata; Edward M. Schwarz
- Source:
- Cell and tissue research 2012 v.347 no.3 pp. 545-552
- ISSN:
- 0302-766X
- Subject:
- allografting; angiogenesis; animal models; animals; arthritis; bone formation; bone resorption; case studies; chondrogenesis; clinical trials; drugs; osteoporosis; parathyroid hormone; patients; resorption; stem cells; tissue engineering
- Abstract:
- ... Based on its proven anabolic effects on bone in osteoporosis patients, recombinant parathyroid hormone (PTH1-34) has been evaluated as a potential therapy for skeletal repair. In animals, the effect of PTH1-34 has been investigated in various skeletal repair models such as fractures, allografting, spinal arthrodesis and distraction osteogenesis. These studies have demonstrated that intermittent PT ...
- DOI:
- 10.1007/s00441-011-1188-4
- PubMed:
- 21626290
- PubMed Central:
- PMC3338097
- https://doi.org/10.1007/s00441-011-1188-4
- Author:
- Ville V. Meretoja; Rebecca L. Dahlin; F. Kurtis Kasper; Antonios G. Mikos
- Source:
- Biomaterials 2012 v.33 no.27 pp. 6362-6369
- ISSN:
- 0142-9612
- Subject:
- DNA; cartilage; cattle; chondrocytes; chondrogenesis; coculture; collagen; cultured cells; engineering; gene expression; models; reverse transcriptase polymerase chain reaction; stem cells
- Abstract:
- ... In this work, articular chondrocytes (ACs) and mesenchymal stem cells (MSCs) with 1:1 and 1:3 cell ratios were co-cultured in order to evaluate if a majority of primary ACs can be replaced with MSCs without detrimental effects on in vitro chondrogenesis. We further used a xenogeneic culture model to study if such co-cultures can result in redifferentiation of passaged ACs. Cells were cultured in p ...
- DOI:
- 10.1016/j.biomaterials.2012.05.042
- PubMed:
- 22695067
- PubMed Central:
- PMC3392514
- https://doi.org/10.1016/j.biomaterials.2012.05.042
- Author:
- Daniel Pelaez; Nestor Arita; Herman S. Cheung
- Source:
- Biochemical and biophysical research communications 2012 v.417 no.4 pp. 1286-1291
- ISSN:
- 0006-291X
- Subject:
- alkaline phosphatase; bone formation; bone marrow; cartilage; chondrogenesis; collagen; fibrin; gels; glycosaminoglycans; mitogen-activated protein kinase; osteocalcin; reverse transcriptase polymerase chain reaction; stem cells
- Abstract:
- ... Elucidating the intracellular signaling cascades which lead to differentiation programs can be a daunting but necessary task. Even more so when the nature of the differentiating stimuli can elicit different biochemical responses yet achieve the same functional outcome. In the field of cartilage and bone regeneration the importance of the extracellular signal-regulated kinase (ERK) pathway has been ...
- DOI:
- 10.1016/j.bbrc.2011.12.131
- https://doi.org/10.1016/j.bbrc.2011.12.131
- Author:
- Tiffany Cheng; Christina Yang; Norbert Weber; Hubert T. Kim; Alfred C. Kuo
- Source:
- Biochemical and biophysical research communications 2012 v.426 no.4 pp. 544-550
- ISSN:
- 0006-291X
- Subject:
- chondrogenesis; fibroblast growth factor 2; gene expression; histology; humans; phenotype; quantitative polymerase chain reaction; stem cells
- Abstract:
- ... Treatment of mesenchymal stem cells (MSCs) with fibroblast growth factor 2 (FGF-2) during monolayer expansion leads to increased expression of cartilage-related molecules during subsequent pellet chondrogenesis. This may be due to faster differentiation and/or a durable change in phenotype. In order to evaluate changes over time, we assessed chondrogenesis of human MSCs at early and late time poin ...
- DOI:
- 10.1016/j.bbrc.2012.08.124
- https://doi.org/10.1016/j.bbrc.2012.08.124
- Author:
- Martha C. Castaño Betancourt; Frederic Cailotto; Hanneke J. Kerkhof; Frederique M. F. Cornelis; Sally A. Doherty; Deborah J. Hart; Albert Hofman; Frank P. Luyten; Rose A. Maciewicz; Massimo Mangino; Sarah Metrustry; Kenneth Muir; Marjolein J. Peters; Fernando Rivadeneira; Maggie Wheeler; Weiya Zhang; Nigel Arden; Tim D. Spector; Andre G. Uitterlinden; Michael Doherty; Rik J. U. Lories; Ana M. Valdes; Joyce B. J. van Meurs
- Source:
- Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.21 pp. 8218-8223
- ISSN:
- 0027-8424
- Subject:
- adults; alleles; cartilage; chondrocytes; chondrogenesis; collagen; histones; homeostasis; immunohistochemistry; leukemia; loci; meta-analysis; mice; mineralization; osteoarthritis; proteoglycans; risk; single nucleotide polymorphism; United Kingdom
- Abstract:
- ... Hip osteoarthritis (HOA) is one of the most disabling and common joint disorders with a large genetic component that is, however, still ill-defined. To date, genome-wide association studies (GWAS) in osteoarthritis (OA) and specifically in HOA have yielded only few loci, which is partly explained by heterogeneity in the OA definition. Therefore, we here focused on radiographically measured joint-s ...
- DOI:
- 10.1073/pnas.1119899109
- PubMed:
- 22566624
- PubMed Central:
- PMC3361426
- https://doi.org/10.1073/pnas.1119899109
- Author:
- Min Jung Kim; Sunhyo Kim; Youngshin Kim; Eun-Jung Jin; Jong Kyung Sonn
- Source:
- Biochemical and biophysical research communications 2012 v.418 no.3 pp. 500-505
- ISSN:
- 0006-291X
- Subject:
- actin; chicks; chondrocytes; chondrogenesis; cytoskeleton; myosin; myosin light chain kinase; phosphorylation
- Abstract:
- ... Cell shape change and cytoskeletal reorganization are known to be involved in the chondrogenesis. Negative role of RhoA, a cytoskeleton-regulating protein, and its downstream target, Rho-associated protein kinase (ROCK) in the chondrogenesis has been studied in many different culture systems including primary chondrocytes, chondrogenic cell lines, dedifferentiated chondrocytes, and micromass cultu ...
- DOI:
- 10.1016/j.bbrc.2012.01.053
- https://doi.org/10.1016/j.bbrc.2012.01.053
- Author:
- Eleonora Iacono; Lara Brunori; Alessandro Pirrone; Pasquale Paolo Pagliaro; Francesca Ricci; Pier Luigi Tazzari; Barbara Merlo
- Source:
- Reproduction 2012 v.143 no.4 pp. 455-468
- ISSN:
- 1470-1626
- Subject:
- alizarin; amnion; amniotic fluid; bone formation; centrifugation; chondrogenesis; droplets; fetal bovine serum; fibroblasts; glycosaminoglycans; immune response; lipogenesis; mares; stem cells; therapeutics; umbilical cord
- Abstract:
- ... Mesenchymal stem cells (MSCs) have been derived from multiple sources of the horse including umbilical cord blood (UCB) and amnion. This work aimed to identify and characterize stem cells from equine amniotic fluid (AF), CB and Wharton's Jelly (WJ). Samples were obtained from 13 mares at labour. AF and CB cells were isolated by centrifugation, while WJ was prepared by incubating with an enzymatic ...
- DOI:
- 10.1530/REP-10-0408
- PubMed:
- 22274885
- https://doi.org/10.1530/REP-10-0408
- Author:
- Sho Mitsugi; Wataru Ariyoshi; Toshinori Okinaga; Takeshi Kaneuji; Yoshihiro Kataoka; Tetsu Takahashi; Tatsuji Nishihara
- Source:
- Biochemical and biophysical research communications 2012 v.420 no.2 pp. 380-384
- ISSN:
- 0006-291X
- Subject:
- Western blotting; cartilage; chemical inhibitors; chondrogenesis; glycosaminoglycans; messenger RNA; mice; mitogen-activated protein kinase; phosphorylation; proteins; quantitative polymerase chain reaction; signal transduction; transforming growth factor beta
- Abstract:
- ... OBJECTIVES: Activin-A, a member of the TGF-β family, is known to be present in bone and cartilage. Although, involvement of the TGF-β family in chondrogenesis has been reported, the mechanism by which activin-A regulates chondrogenesis has not been fully elucidated. The aim of this study was to investigate the effects of activin-A on chondrocyte differentiation in vitro. MATERIALS AND METHODS: Mon ...
- DOI:
- 10.1016/j.bbrc.2012.03.003
- https://doi.org/10.1016/j.bbrc.2012.03.003
- Author:
- Rocha Beatriz; Calamia Valentina; Mateos Jesús; Fernández-Puente Patricia; Blanco Francisco J.; Ruiz-Romero Cristina
- Source:
- Journal of Proteome Research 2012 v.11 no.11 pp. 5350-5361
- ISSN:
- 1535-3907
- Subject:
- amino acids; bone marrow; cartilage; cell adhesion; cell culture; chondrocytes; chondrogenesis; cyclophilins; extracellular matrix; fibronectins; humans; immunohistochemistry; isotope labeling; metabolism; mitochondria; osteoarthritis; proteome; proteomics; quantitative analysis; stable isotopes; stem cells; stress response; superoxide dismutase; vimentin
- Abstract:
- ... Human mesenchymal stem cells (hMSCs), residing in bone marrow as well as in the synovial lining of joints, can be triggered to differentiate toward chondrocytes. Thus, hMSCs harbor great therapeutic potential for the repair of cartilage defects in osteoarthritis (OA) and other articular diseases. However, the molecular mechanisms underlying the chondrogenesis process are still in part unknown. In ...
- DOI:
- 10.1021/pr300572r
- https://doi.org/10.1021/pr300572r
- Author:
- Shiwu Dong; Bo Yang; Hongfeng Guo; Fei Kang
- Source:
- Biochemical and biophysical research communications 2012 v.418 no.4 pp. 587-591
- ISSN:
- 0006-291X
- Subject:
- bone diseases; bone formation; cartilage; chondrocytes; chondrogenesis; developmental orthopedic disease; gene expression; gene therapy; genes; microRNA; osteoblasts; osteoclasts
- Abstract:
- ... MicroRNAs (miRNAs) are a class of small molecules and non-coding single strand RNAs that regulate gene expression at the post-transcriptional level by binding to specific sequences within target genes. miRNAs have been recognized as important regulatory factors in organism development and disease expression. Some miRNAs regulate the proliferation and differentiation of osteoblasts, osteoclasts and ...
- DOI:
- 10.1016/j.bbrc.2012.01.075
- https://doi.org/10.1016/j.bbrc.2012.01.075
- Author:
- Wei Seong Toh; Teck Chuan Lim; Motoichi Kurisawa; Myron Spector
- Source:
- Biomaterials 2012 v.33 no.15 pp. 3835-3845
- ISSN:
- 0142-9612
- Subject:
- crosslinking; contracture; biosynthesis; phenotype; morphogenesis; biomimetics; hydrocolloids; goats; hydrogen peroxide; chondrogenesis; cartilage; collagen; stem cells; oxidants; biodegradability; tissue engineering; hyaluronic acid; peroxidase; gelation
- Abstract:
- ... An injectable and biodegradable hydrogel system comprising hyaluronic acid-tyramine (HA-Tyr) conjugates can safely undergo covalent cross-linking in vivo by the addition of small amounts of peroxidase and hydrogen peroxide (H₂O₂), with the independent tuning of the gelation rate and degree of cross-linking. Such hydrogel networks with tunable mechanical and degradation properties may provide the a ...
- DOI:
- 10.1016/j.biomaterials.2012.01.065
- https://doi.org/10.1016/j.biomaterials.2012.01.065
- Author:
- Elise Duval; Catherine Baugé; Rina Andriamanalijaona; Hervé Bénateau; Sylvain Leclercq; Soizic Dutoit; Laurent Poulain; Philippe Galéra; Karim Boumédiene
- Source:
- Biomaterials 2012 v.33 no.26 pp. 6042-6051
- ISSN:
- 0142-9612
- Subject:
- biotechnology; bone marrow; cartilage; chondrocytes; chondrogenesis; cultured cells; gene overexpression; growth factors; humans; hypoxia; medicine; mice; phenotype; stem cells; tissue analysis; tissue engineering; transcription factors
- Abstract:
- ... Cartilage engineering is one of the most challenging issue in regenerative medicine, due to its limited self-ability to repair. Here, we assessed engineering of cartilage tissue starting from human bone marrow (hBM) stem cells under hypoxic environment and delineated the mechanism whereby chondrogenesis could be conducted without addition of exogenous growth factors. hBM stem cells were cultured i ...
- DOI:
- 10.1016/j.biomaterials.2012.04.061
- https://doi.org/10.1016/j.biomaterials.2012.04.061
- Author:
- Robert P. Gersch; Arif Kirmizitas; Lidia Sobkow; Gina Sorrentino; Gerald H. Thomsen; Michael Hadjiargyrou
- Source:
- Gene expression patterns 2012 v.12 no.3-4 pp. 145-153
- ISSN:
- 1567-133X
- Subject:
- Xenopus laevis; cartilage; chondrogenesis; embryogenesis; eyes; gene expression; head; in situ hybridization; messenger RNA; muscle development; pharynx; phenotype; reverse transcriptase polymerase chain reaction; swimming; tadpoles; tail
- Abstract:
- ... Mustn1 is a vertebrate-specific protein that, in vitro, was showed to be essential for prechondrocyte function and thus it has the potential to regulate chondrogenesis during embryonic development. We use Xenopus laevis as a model to examine Mustn1 involvement in chondrogenesis. Previous work suggests that Mustn1 is necessary but not sufficient for chondrogenic proliferation and differentiation, a ...
- DOI:
- 10.1016/j.gep.2012.01.002
- PubMed:
- 22281807
- PubMed Central:
- PMC3348343
- https://doi.org/10.1016/j.gep.2012.01.002
- Author:
- Karri L. Bertram; Roman J. Krawetz
- Source:
- Biochemical and biophysical research communications 2012 v.422 no.3 pp. 455-461
- ISSN:
- 0006-291X
- Subject:
- adults; cartilage; chondrogenesis; gene expression; genes; osmolarity; osteoarthritis; patients; quantitative polymerase chain reaction; rheumatoid arthritis; stem cells; synovial fluid
- Abstract:
- ... Cartilage is one of few tissues where adult stem/progenitor cells have not been putatively identified. Recent studies have provided strong evidence that a sub-population of mesenchymal progenitor cells (MPCs) derived from the synovial fluid may be able to affect some degree of cartilage repair both in vivo and in vitro/ex vivo, however this does not appear to be the case in patients with arthritis ...
- DOI:
- 10.1016/j.bbrc.2012.05.015
- https://doi.org/10.1016/j.bbrc.2012.05.015
- Author:
- Eamon J. Sheehy; Conor T. Buckley; Daniel J. Kelly
- Source:
- Biochemical and biophysical research communications 2012 v.417 no.1 pp. 305-310
- ISSN:
- 0006-291X
- Subject:
- alizarin; alkaline phosphatase; bone formation; bone marrow; cartilage; chondrogenesis; collagen; hydrocolloids; hypertrophy; oxygen; pellets; phenotype; stem cells; tissue engineering
- Abstract:
- ... The local oxygen tension is a key regulator of the fate of mesenchymal stem cells (MSCs). The objective of this study was to investigate the effect of a low oxygen tension during expansion and differentiation on the proliferation kinetics as well as the subsequent osteogenic and chondrogenic potential of MSCs. We first hypothesised that expansion in a low oxygen tension (5% pO₂) would improve both ...
- DOI:
- 10.1016/j.bbrc.2011.11.105
- https://doi.org/10.1016/j.bbrc.2011.11.105
- Author:
- Jong-Min Lee; Gun-Il Im
- Source:
- Biochemical and biophysical research communications 2012 v.421 no.4 pp. 819-824
- ISSN:
- 0006-291X
- Subject:
- DNA; alkaline phosphatase; bone marrow; cartilage; chondrogenesis; genes; hypertrophy; in vitro culture; pellets; protein synthesis; stem cells; tissue engineering; transforming growth factors
- Abstract:
- ... While several isoforms of parathyroid hormone-related peptide (PTHrP) have been commercially available, the difference in their effect has not been widely studied. The purpose of this study was to determine which isoform most effectively promoted chondrogenesis and suppressed hypertrophy from mesenchymal stem cells (MSCs). MSCs isolated from fresh bone marrow were cultured in pellet in chondrogeni ...
- DOI:
- 10.1016/j.bbrc.2012.04.096
- https://doi.org/10.1016/j.bbrc.2012.04.096
35. SOX9 and myocardin counteract each other in regulating vascular smooth muscle cell differentiation
- Author:
- Zhonghui Xu; Guangdong Ji; Jianbin Shen; Xiaobo Wang; Jiliang Zhou; Li Li
- Source:
- Biochemical and biophysical research communications 2012 v.422 no.2 pp. 285-290
- ISSN:
- 0006-291X
- Subject:
- actin; atherosclerosis; blood serum; calcification; cell differentiation; chondrocytes; chondrogenesis; gene overexpression; genes; genetic markers; luciferase; myocytes; pathogenesis; reverse transcriptase polymerase chain reaction; smooth muscle; transcription (genetics)
- Abstract:
- ... Transdifferentiation of vascular smooth muscle cells (VSMC) into chondrogenic cells contributes significantly to vascular calcification during the pathogenesis of atherosclerosis. However, the transcriptional mechanisms that control such phenotypic switch remain unclear. This process is characterized by the induction of Sox9 and Col2a1 genes accompanied by the repression of myocardin (Myocd) and S ...
- DOI:
- 10.1016/j.bbrc.2012.04.149
- PubMed:
- 22580282
- PubMed Central:
- PMC3448976
- https://doi.org/10.1016/j.bbrc.2012.04.149
- Author:
- Ji Sun Park; Han Na Yang; Dae Gyun Woo; Su Yeon Jeon; Keun-Hong Park
- Source:
- Biomaterials 2012 v.33 no.29 pp. 7151-7163
- ISSN:
- 0142-9612
- Subject:
- adhesion; chondrogenesis; coatings; collagen; dexamethasone; extracellular matrix; genes; in vitro culture; stem cells; therapeutics; tissue engineering; tissues; transforming growth factor beta 3
- Abstract:
- ... Microparticulated types of scaffolds have been widely applied in stem cell therapy and the tissue engineering field for the regeneration of wound tissues. During application of simple genes or growth factors and cell delivery vehicles, we designed a method that employs dexamethsone loaded PLGA microspheres consisting of polyplexed SOX9 genes plus heparinized TGF-β 3 on the surface of polymeric mic ...
- DOI:
- 10.1016/j.biomaterials.2012.06.023
- https://doi.org/10.1016/j.biomaterials.2012.06.023
- Author:
- Natalina Quarto; Brian Leonard; Shuli Li; Melanie Marchand; Erica Anderson; Barry Behr; Uta Francke; Renee Reijo-Pera; Eric Chiao; Michael T. Longaker
- Source:
- Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.1 pp. 215-220
- ISSN:
- 0027-8424
- Subject:
- bone formation; chondrogenesis; embryonic stem cells; extracellular matrix; fibroblasts; genes; humans; induced pluripotent stem cells; mutants; mutation; pathogenesis; patients; phenotype; transforming growth factor beta
- Abstract:
- ... Marfan syndrome (MFS) is a heritable connective tissue disorder caused by mutations in the gene coding for FIBRILLIN-1 (FBN1), an extracellular matrix protein. MFS is inherited as an autosomal dominant trait and displays major manifestations in the ocular, skeletal, and cardiovascular systems. Here we report molecular and phenotypic profiles of skeletogenesis in tissues differentiated from human e ...
- DOI:
- 10.1073/pnas.1113442109
- PubMed:
- 22178754
- PubMed Central:
- PMC3252902
- https://doi.org/10.1073/pnas.1113442109
- Author:
- Carla M.R. Lacerda; Holly B. MacLea; John D. Kisiday; E. Christopher Orton
- Source:
- Journal of veterinary cardiology 2012 v.14 no.1 pp. 223-230
- ISSN:
- 1760-2734
- Subject:
- cardiovascular diseases; chondrogenesis; dogs; enzyme-linked immunosorbent assay; enzymes; human diseases; immunoblotting; phenotype; protein synthesis; proteins; serotonin; tryptophan
- Abstract:
- ... OBJECTIVES: Degenerative (myxomatous) mitral valve disease is an important cardiac disease in dogs and humans. The mechanisms that initiate and propagate myxomatous pathology in mitral valves are poorly understood. We investigated the hypothesis that tensile strain initiates expression of proteins that mediate myxomatous pathology. We also explored whether tensile strain could induce the serotonin ...
- DOI:
- 10.1016/j.jvc.2011.12.002
- PubMed:
- 22364693
- https://doi.org/10.1016/j.jvc.2011.12.002
- Author:
- Kristen Johnson; Shoutian Zhu; Matthew S. Tremblay; Joshua N. Payette; Jianing Wang; Laure C. Bouchez; Shelly Meeusen; Alana Althage; Charles Y. Cho; Xu Wu; Peter G. Schultz
- Source:
- Science 2012 v.336 no.6082 pp. 717-721
- ISSN:
- 0036-8075
- Subject:
- animal models; cartilage; chondrocytes; chondrogenesis; filamin; median effective concentration; osteoarthritis; stem cells; therapeutics; transcription (genetics); transcription factors
- Abstract:
- ... Osteoarthritis (OA) is a degenerative joint disease that involves the destruction of articular cartilage and eventually leads to disability. Molecules that promote the selective differentiation of multipotent mesenchymal stem cells (MSCs) into chondrocytes may stimulate the repair of damaged cartilage. Using an image-based high-throughput screen, we identified the small molecule kartogenin, which ...
- DOI:
- 10.1126/science.1215157
- https://doi.org/10.1126/science.1215157
- Author:
- Hyuck Joon Kwon
- Source:
- Biochemical and biophysical research communications 2012 v.424 no.4 pp. 793-800
- ISSN:
- 0006-291X
- Subject:
- calcium channels; calcium; chondrogenesis; adenosine triphosphate; adhesion; transforming growth factor beta 1
- Abstract:
- ... Although both TGF-β and BMP signaling enhance expression of adhesion molecules during chondrogenesis, TGF-β but not BMP signaling can initiate condensation of uncondensed mesenchymal cells. However, it remains unclear what causes the differential effects between TGF-β and BMP signaling on prechondrogenic condensation. Our previous report demonstrated that ATP oscillations play a critical role in p ...
- DOI:
- 10.1016/j.bbrc.2012.07.041
- https://doi.org/10.1016/j.bbrc.2012.07.041
- Author:
- Christine Y. Chuang; Kifah Shahin; Megan S. Lord; James Melrose; Pauline M. Doran; John M. Whitelock
- Source:
- Biomaterials 2012 v.33 no.16 pp. 4078-4088
- ISSN:
- 0142-9612
- Subject:
- cartilage; cell growth; chondrocytes; chondrogenesis; collagen; fibroblast growth factors; humans; osteoarthritis; phenotype; quality of life; sodium alginate
- Abstract:
- ... Development of cartilage lesions in osteoarthritis and following traumatic injury has important consequences on the weight bearing and articulation of joints, has severe impact on the quality of life of affected individuals and is of significant socioeconomic impact. Hyaline cartilage is a highly specialised tissue with a limited ability to self repair. Development of three-dimensional scaffolds w ...
- DOI:
- 10.1016/j.biomaterials.2012.02.032
- https://doi.org/10.1016/j.biomaterials.2012.02.032
- Author:
- Jiewen Dai; Jia Wang; Jingting Lu; Duohong Zou; Hao Sun; Yuefu Dong; Hongbo Yu; Lei Zhang; Tong Yang; Xiuli Zhang; Xudong Wang; Guofang Shen
- Source:
- Biomaterials 2012 v.33 no.31 pp. 7699-7711
- ISSN:
- 0142-9612
- Subject:
- bone formation; cartilage; chondrocytes; chondrogenesis; coculture; fibroblast growth factor receptor 3; humans; mineralization; neural crest; phosphorylation; stem cells; tissue engineering; tooth pulp
- Abstract:
- ... Dental pulp stem cells (DPSCs), which arise from cranial neural crest cells, are multipotent, making them a candidate for use in tissue engineering that may be especially useful for craniofacial tissues. Costal chondrocytes (CCs) can be easily obtained and demonstrate higher initial cell yields and expansion than articular chondrocytes. CCs have been found to retain chondrogenic capacity that can ...
- DOI:
- 10.1016/j.biomaterials.2012.07.020
- https://doi.org/10.1016/j.biomaterials.2012.07.020
- Author:
- Juan Li; Jun Wang; Yuanwen Zou; Yifan Zhang; Dan Long; Lei Lei; Lijun Tan; Rui Ye; Xuejin Wang; Zhihe Zhao
- Source:
- Biomaterials 2012 v.33 no.33 pp. 8395-8405
- ISSN:
- 0142-9612
- Subject:
- chondrogenesis; gene expression; mechanical stress; messenger RNA; protein phosphorylation; protein synthesis; rats; transcription (genetics); transforming growth factor beta 1
- Abstract:
- ... Mechanical stimuli play important roles in regulating chondrogenic differentiation, but seldom studies have focused on when and how mechanical stimuli should be initiated. We have previously shown that Col2α1 mRNA was increased by delayed dynamic compressive stress initiated at the 8th day of chondrogenic culture. The current work is to further study the possibility of using delayed mechanical str ...
- DOI:
- 10.1016/j.biomaterials.2012.08.019
- https://doi.org/10.1016/j.biomaterials.2012.08.019
- Author:
- Paul H. Ousema; Franklin T. Moutos; Bradley T. Estes; Arnold I. Caplan; Donald P. Lennon; Farshid Guilak; J. Brice Weinberg
- Source:
- Biomaterials 2012 v.33 no.35 pp. 8967-8974
- ISSN:
- 0142-9612
- Subject:
- biomechanics; biomimetics; cartilage; chondrogenesis; collagen; extracellular matrix; in vitro culture; interleukin-1; mechanical properties; normal values; proteoglycans; stem cells
- Abstract:
- ... Tissue-engineered constructs designed to treat large cartilage defects or osteoarthritic lesions may be exposed to significant mechanical loading as well as an inflammatory environment upon implantation in an injured or diseased joint. We hypothesized that a three-dimensionally (3D) woven poly(ε-caprolactone) (PCL) scaffold seeded with bone marrow-derived mesenchymal stem cells (MSCs) would provid ...
- DOI:
- 10.1016/j.biomaterials.2012.08.045
- PubMed:
- 22999467
- PubMed Central:
- PMC3466362
- https://doi.org/10.1016/j.biomaterials.2012.08.045
- Author:
- BEHNAZ BAKHSHANDEH; MASOUD SOLEIMANI; SEYED HASSAN PAYLAKHI; NASSER GHAEMI
- Source:
- Journal of genetics 2012 v.91 no.2 pp. 171-182
- ISSN:
- 0022-1333
- Subject:
- cadherins; cartilage; chondrocytes; chondrogenesis; gene expression regulation; genetic markers; guidelines; immunocytochemistry; microRNA; microarray technology; mitogen-activated protein kinase; mitogen-activated protein kinase kinase kinase; quantitative polymerase chain reaction; stem cells; transforming growth factor beta
- Abstract:
- ... Generating appropriate cartilage for clinical applications to heal skeletal tissue loss is a major health concern. In this regard, cell-based approaches offer a potential therapeutic strategy for cartilage repair, although little is known about the precise mechanism of chondrogenesis. Unrestricted somatic stem cell (USSC) is considered as a suitable candidate because of its potential for different ...
- DOI:
- 10.1007/s12041-012-0168-0
- https://doi.org/10.1007/s12041-012-0168-0
- Author:
- Wei-Hong Chen; Rong Zeng; Wen-Cheng Lo; Szu-Yu Tina Chen; Tung-Yuan Lai; David F. Williams; Win-Ping Deng
- Source:
- Biomaterials 2012 v.33 no.33 pp. 8256-8264
- ISSN:
- 0142-9612
- Subject:
- calcitonin; calcitonin receptors; chondrogenesis; cyclic AMP; humans; phenotype; signal transduction; therapeutics
- Abstract:
- ... Human disc degeneration initiated by aging in the central nucleus pulposus (hNP) is an irreversible process and the recovery has become seriously emerging. In this study, the related mechanisms of calcitonin on the regeneration of hNP and the effects of calcitonin on the age-related alterations were examined. The harvested hNP population was designated as YhNP (from young donor, age <50) and OhNP ...
- DOI:
- 10.1016/j.biomaterials.2012.06.088
- https://doi.org/10.1016/j.biomaterials.2012.06.088
- Author:
- J. A. Gillis; M. S. Modrell; C. V. H. Baker
- Source:
- Journal of applied ichthyology 2012 v.28 no.3 pp. 341-345
- ISSN:
- 0175-8659
- Subject:
- Danio rerio; Polyodon spathula; Scyliorhinus canicula; ancestry; cartilage; chicks; chondrogenesis; gills; jaws; mice; models; phylogeny; sharks
- Abstract:
- ... The lesser‐spotted dogfish (Scyliorhinus canicula) and the North American paddlefish (Polyodon spathula) are two emerging model systems for the study of vertebrate craniofacial development. Notably, both of these taxa have retained plesiomorphic aspects of pharyngeal endoskeletal organization, relative to more commonly used models of vertebrate craniofacial development (e.g. zebrafish, chick and m ...
- DOI:
- 10.1111/j.1439-0426.2012.01976.x
- PubMed:
- 26566297
- PubMed Central:
- PMC4640176
- https://doi.org/10.1111/j.1439-0426.2012.01976.x