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You searched for: Publication Year 2012 Remove constraint Publication Year: 2012 Subject cyclic AMP Remove constraint Subject: cyclic AMP Text Availability Citation in PubAg Remove constraint Text Availability: Citation in PubAg
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1. 1-Bromopropane up-regulates cyclooxygenase-2 expression via NF-κB and C/EBP activation in murine macrophages

Author:
Eun Hee Han; Ji Hye Yang; Hyung-Kyun Kim; Jae Ho Choi; Tilak Khanal; Minh Truong Do; Young Chul Chung; Kwang Youl Lee; Tae Cheon Jeong; Hye Gwang Jeong
Source:
Food and chemical toxicology 2012 v.50 no.5 pp. 1616-1622
ISSN:
0278-6915
Subject:
binding proteins; cyclic AMP; gene expression; humans; industry; luciferase; macrophages; messenger RNA; metabolites; mice; mitogen-activated protein kinase; prostaglandin synthase; prostaglandins; signal transduction; solvents; toxicology; transcription factor NF-kappa B; transfection
Abstract:
... 1-Bromopropane (1-BP) has been used in industry as an alternative to ozone-depleting solvents. In the present study, we examined the effect of 1-BP on cyclooxygenase-2 (COX-2) gene expression and analyzed the molecular mechanism of its activity in murine RAW 264.7 macrophages. 1-BP dose-dependently increased COX-2 protein and mRNA levels, as well as COX-2 promoter-driven luciferase activity in mac ...
DOI:
10.1016/j.fct.2012.02.006

2. 20β-hydroxysteroid dehydrogenase gene promoter: Potential role for cyclic AMP and xenobiotic responsive elements

Author:
G. Sreenivasulu; B. Senthilkumaran; C.C. Sudhakumari; G. Guan; Y. Oba; H. Kagawa; Y. Nagahama
Source:
Gene 2012 v.509 no.1 pp. 68-76
ISSN:
0378-1119
Subject:
Oncorhynchus mykiss; TATA box; catfish; cyclic AMP; drugs; forskolin; genes; hormone metabolism; luciferase; mammals; oocytes; steroid hormones; substrate specificity; transcription (genetics); transcription factors; trout; xenobiotics
Abstract:
... Teleostean 20β-hydroxysteroid dehydrogenase (20β-HSD) is involved in final oocyte maturation and steroid hormone metabolism. It has structural and functional similarities to mammalian carbonyl reductases that are involved in the metabolism of endogenous carbonyl and xenobiotic compounds. To understand the transcriptional regulation of 20β-HSD, here we report the cloning of 20β-HSD promoter from tw ...
DOI:
10.1016/j.gene.2012.07.017
PubMed:
22835697

3. Acidosis-induced V-ATPase trafficking in salivary ducts is initiated by cAMP/PKA/CREB pathway via regulation of Rab11b expression

Author:
Oliver Oehlke; Cornelius Schlosshardt; Melanie Feuerstein; Eleni Roussa
Source:
international journal of biochemistry & cell biology 2012 v.44 no.8 pp. 1254-1265
ISSN:
1357-2725
Subject:
H-transporting ATP synthase; acidosis; cAMP-dependent protein kinase; cyclic AMP; genes; homeostasis; humans; immunoblotting; mitogen-activated protein kinase; plasma membrane; protein phosphorylation; reverse transcriptase polymerase chain reaction; salivary ducts; signal transduction; transporters
Abstract:
... Changes in systemic acid–base homeostasis cause a series of organ-specific cellular responses, among them changes of acid–base transporter activities, and recruitment or retrieval of these transporters from intracellular pools to the plasma membrane and vice versa. The purpose of this study was to investigate the impact of protein phosphorylation in the acidosis-induced translocation of vacuolar-t ...
DOI:
10.1016/j.biocel.2012.04.018

4. Activation of ERK1/2-CREB pathway during potentiating synaptic transmission of ( − )clausenamide in rat dentate gyrus

Author:
Jin-Feng Hu; Fei Niu; Na Ning; Wen-Zhen Duan; Shi-Feng Chu; Wei Xue; Yu-He Yuan; Nai-Hong Chen; Jun-Tian Zhang
Source:
Journal of Asian natural products research 2012 v.14 no.3 pp. 256-262
ISSN:
1477-2213
Subject:
Western blotting; cortex; cyclic AMP; electrophysiology; hippocampus; immunohistochemistry; in situ hybridization; memory; messenger RNA; mitogen-activated protein kinase; phosphorylation; population growth; rats; synaptic transmission
Abstract:
... To investigate the signal mechanism of ( − )clausenamide (( − )-3-hydroxy-5-(hydroxy-phenyl-methyl)-1-methyl-4-phenyl-pyrrolidin-2-one, 1) and for understanding its effect on synaptic transmission, electrophysiological recording was done for basal synaptic transmission determination. Western blot analysis was employed to examine the phosphorylation of extracellular signal-regulated kinase 1/2 (ERK ...
DOI:
10.1080/10286020.2011.650885

5. Activators of protein kinase A and oxytocin affect rabbit reproduction

Author:
Andrej Balazi; Alexander Sirotkin; Peter Chrenek
Source:
Open life sciences 2012 v.7 no.6 pp. 973-979
ISSN:
2391-5412
Subject:
blastocyst; cAMP-dependent protein kinase; conception rate; corpus luteum; cyclic AMP; eggs; equine chorionic gonadotropin; females; follicular development; hatching; human chorionic gonadotropin; insemination; mortality; morula; oviducts; ovulation; oxytocin; parturition; pups; rabbits; signal transduction; weaning; zygote
Abstract:
... The aim of this study was to examine the effect of activators of protein kinase A — 3-isobutyl-1-methyl-xanthine (IBMX) and dibutyryl cyclic adenosine monophosphate (dbcAMP) — and of oxytocin (OT) on rabbit female reproductive function. We used equine chorionic gonadotropin (eCG) to improve follicular development and rabbit estrous synchronization and human chorionic gonadotropin (hCG) to induce o ...
DOI:
10.2478/s11535-012-0089-1

6. Airway epithelial cells—Functional links between CFTR and anoctamin dependent Cl⁻ secretion

Author:
Karl Kunzelmann; Yuemin Tian; Joana Raquel Martins; Diana Faria; Patthara Kongsuphol; Jiraporn Ousingsawat; Luisa Wolf; Rainer Schreiber
Source:
international journal of biochemistry & cell biology 2012 v.44 no.11 pp. 1897-1900
ISSN:
1357-2725
Subject:
calcium; chlorides; cyclic AMP; cystic fibrosis; electrolytes; goblet cells; ion channels; lungs; secretion
Abstract:
... Airways consist of a heterogeneous population of cells, comprising ciliated cells, Clara cells and goblet cells. Electrolyte secretion by the airways is necessary to produce the airway surface liquid that allows for mucociliary clearance of the lungs. Secretion is driven by opening of Cl⁻ selective ion channels in the apical membrane of airway epithelial cells, through either receptor mediated inc ...
DOI:
10.1016/j.biocel.2012.06.011

7. Allosteric Control of cAMP Receptor Binding Dynamics

Author:
Porschke Dietmar
Source:
Biochemistry 2012 v.51 no.19 pp. 4028-4034
ISSN:
1520-4995
Subject:
DNA; adenosine monophosphate; binding proteins; cyclic AMP; fluorescence; pH
Abstract:
... The intrinsic fluorescence of the cyclic AMP receptor is a sensitive indicator of the reaction with DNA, but signals are perturbed by a photoreaction. A ratio procedure is shown to be useful for correction. The reaction of the protein with DNA indicated by corrected transients extends over a broad time range not only at low salt concentrations but also at physiological salt concentrations. The ini ...
DOI:
10.1021/bi3002874

8. Antidepressant-like effect of hyperoside isolated from Apocynum venetum leaves: Possible cellular mechanisms

Author:
Meizhu Zheng; Chunming Liu; Fengguang Pan; Dongfang Shi; Yuchi Zhang
Source:
Phytomedicine 2012 v.19 no.2 pp. 145-149
ISSN:
0944-7113
Subject:
Apocynum; binding proteins; calcium; cell viability; corticosterone; cyclic AMP; leaves; models; neurotoxicity; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; signal transduction; tetrazolium
Abstract:
... In the present work, we studied the possible cellular mechanisms of hyperoside isolated from Apocynum venetum leaves in corticosterone-induced neurotoxicity, using PC12 cells as a suitable in vitro model of depression. Cell viability was quantitated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. The release amount of lactic dehydrogenase (LDH) and intracellular Ca²⁺ ...
DOI:
10.1016/j.phymed.2011.06.029

9. B-cell translocation gene-2 increases hepatic gluconeogenesis via induction of CREB

Author:
Seung-Lark Hwang; Okyun Kwon; Soo Jin Lee; Seong-Soo Roh; Yong Deuk Kim; Jang Hyun Choi
Source:
Biochemical and biophysical research communications 2012 v.427 no.4 pp. 801-805
ISSN:
0006-291X
Subject:
B-lymphocytes; adenosine monophosphate; cyclic AMP; dexamethasone; gene expression; gene expression regulation; genes; gluconeogenesis; glucose; glucose-6-phosphatase; hepatocytes; insulin; transcription (genetics); transcription factors
Abstract:
... Hepatic gluconeogenesis is mediated by the network of transcriptional factors and cofactors. Here, we show that B-cell translocation gene-2 (BTG2) plays a crucial cofactor in hepatic gluconeogenesis via upregulation of the cyclic AMP (cAMP) response element binding (CREB) in hepatocytes. cAMP/dexamethasone (Dex) significantly increased BTG2 and other gluconeogenic genes such as Nur77, phosphoenolp ...
DOI:
10.1016/j.bbrc.2012.09.146

10. Bi-stage control of dissolved oxygen to enhance cyclic adenosine monophosphate production by Arthrobacter A302

Author:
Lei Li; Xiaochun Chen; Jian Cheng; Chunwei Zhang; Jianxin Bai; Yong Chen; Huanqin Niu; Hanjie Ying
Source:
Bioprocess and biosystems engineering 2012 v.35 no.8 pp. 1281-1286
ISSN:
1615-7591
Subject:
Arthrobacter; batch fermentation; cyclic AMP; dissolved oxygen
Abstract:
... Experiments confirmed dissolved oxygen (DO) definitely affects cyclic adenosine monophosphate (cAMP) production by Arthrobacter A302. Production of cAMP by batch fermentation was investigated under various DO conditions. A two-stage DO control strategy was proposed to achieve optimal production of cAMP based on the kinetic analysis: the DO level was controlled at 40% during the first 18 h and the ...
DOI:
10.1007/s00449-012-0715-3

11. CFTR mediated chloride secretion in the avian renal proximal tubule

Author:
Gary Laverty; Ashley Anttila; Jenava Carty; Varudhini Reddy; Jamie Yum; Sighvatur S. Árnason
Source:
Comparative biochemistry and physiology 2012 v.161 no.1 pp. 53-60
ISSN:
1095-6433
Subject:
Gallus gallus; birds; cell culture; chlorides; cyclic AMP; electrophysiology; forskolin; immunoblotting; nystatin; parathyroid hormone; proximal tubules; reverse transcriptase polymerase chain reaction; secretion
Abstract:
... In primary cell cultures of the avian (Gallus gallus) renal proximal tubule parathyroid hormone and cAMP activation generate a Cl⁻-dependent short circuit current (ISC) response, consistent with net transepithelial Cl⁻ secretion. In this study we investigated the expression and physiological function of the Na–K–2Cl (NKCC) transporter and CFTR chloride channel, both associated with Cl⁻ secretion i ...
DOI:
10.1016/j.cbpa.2011.09.005

12. Calcium/calmodulin and cAMP/protein kinase-A pathways regulate sperm motility in the stallion

Author:
Jodi Lasko; Karen Schlingmann; Ann Klocke; Grace Ann Mengel; Regina Turner
Source:
Animal reproduction science 2012 v.132 no.3-4 pp. 169-177
ISSN:
0378-4320
Subject:
acrosome; adenosine monophosphate; agonists; calcium; calmodulin; cyclic AMP; fluorescent antibody technique; immunoblotting; protein kinases; sperm motility; stallions; viability
Abstract:
... In spite of the importance of sperm motility to fertility in the stallion, little is known about the signaling pathways that regulate motility in this species. In other mammals, calcium/calmodulin signaling and the cyclic AMP/protein kinase-A pathway are involved in sperm motility regulation. We hypothesized that these pathways also were involved in the regulation of sperm motility in the stallion ...
DOI:
10.1016/j.anireprosci.2012.05.007

13. Characterization of a novel tyrosinase inhibitor, (2RS,4R)-2-(2,4-dihydroxyphenyl)thiazolidine-4-carboxylic acid (MHY384)

Author:
Yu Kyeong Han; Yun Jung Park; Young Mi Ha; Daeui Park; Ji Yeon Lee; Naree Lee; Jeong Hyun Yoon; Hyung Ryong Moon; Hae Young Chung
Source:
Biochimica et biophysica acta 2012 v.1820 no.4 pp. 542-549
ISSN:
0304-4165
Subject:
active sites; alpha-melanocyte-stimulating hormone; carbon; catalytic activity; cyclic AMP; gene expression; gene expression regulation; mass spectrometry; mechanism of action; melanin; melanogenesis; melanoma; messenger RNA; models; mushrooms; nuclear magnetic resonance spectroscopy; spectral analysis; stable isotopes; transcription factors
Abstract:
... BACKGROUND: We synthesized (2RS,4R)-2-(2,4-dihydroxyphenyl)thiazolidine-4-carboxylic acid (MHY384) as a potential tyrosinase inhibitor and investigated its antityrosinase activity. METHODS: The structure of MHY384 was established using ¹H and ¹³C NMR spectroscopy and mass spectral analyses. To investigate dual mechanisms of action of MHY384 for the inhibition of melanin synthesis, we confirmed the ...
DOI:
10.1016/j.bbagen.2012.01.001

14. Characterization of ligand response properties of the CRP protein from Pseudomonas putida

Author:
Feng Jiang; ZheXian Tian; YiPing Wang
Source:
Chinese science bulletin 2012 v.57 no.30 pp. 3878-3885
ISSN:
1001-6538
Subject:
Escherichia coli; Pseudomonas putida; bacteria; binding properties; calorimetry; carbon; cyclic AMP; cyclic GMP; metabolism; mutants; operon; proteins; sequence alignment; serine; titration; transcriptional activation
Abstract:
... cAMP receptor protein (CRP) plays profound roles in many bacteria as a global regulator. In Escherichia coli, CRP E. coli modulates the expression of many operons involved in carbon catabolism, in response to the fluctuation of intracellular cAMP level caused by carbon catabolism. A crp homologue gene has been identified in the genome of Pseudomonas putida, however, little is known about its cellu ...
DOI:
10.1007/s11434-012-5360-3

15. Cilomilast enhances osteoblast differentiation of mesenchymal stem cells and bone formation induced by bone morphogenetic protein 2

Author:
Maria Chiara Munisso; Jeong-Hun Kang; Makoto Tsurufuji; Tetsuji Yamaoka
Source:
Biochimie 2012 v.94 no.11 pp. 2360-2365
ISSN:
0300-9084
Subject:
alkaline phosphatase; bone formation; bone morphogenetic proteins; cyclic AMP; gene expression; mice; orthopedics; osteocalcin; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; signal transduction; stem cells
Abstract:
... A rapid and efficient method to stimulate bone regeneration would be useful in orthopaedic stem cell therapies. Rolipram is an inhibitor of phosphodiesterase 4 (PDE4), which mediates cyclic adenosine monophosphate (cAMP) degradation. Systemic injection of rolipram enhances osteogenesis induced by bone morphogenetic protein 2 (BMP-2) in mice. However, there is little data on the precise mechanism, ...
DOI:
10.1016/j.biochi.2012.05.031

16. Cognition-enhancing and neuroprotective activities of the standardized extract of Betula platyphylla bark and its major diarylheptanoids

Author:
Ki Yong Lee; Eun Ju Jeong; Jungmoo Huh; Namki Cho; Tae Bum Kim; Byung Ju Jeon; Seung Hyun Kim; Hong Pyo Kim; Sang Hyun Sung
Source:
Phytomedicine 2012 v.19 no.14 pp. 1315-1320
ISSN:
0944-7113
Subject:
Alzheimer disease; Betula; adenosine monophosphate; bark; body weight; cell death; cortex; cyclic AMP; glutamic acid; hippocampus; memory; mice; neurodegenerative diseases; neurons; neuroprotective effect; neurotoxicity; oral administration; phosphorylation
Abstract:
... Diarylheptanoids have been the center of the intensive research efforts for Alzheimer's disease and other neurodegenerative diseases. The present study aimed to determine the effect of the standardized extract of B. platyphylla bark and its major diarylheptanoids in scopolamine-induced amnesic mice through cyclic AMP response element-binding protein (CREB) activation. Oral administration of the st ...
DOI:
10.1016/j.phymed.2012.09.012

17. Comparative proteomic analysis to dissect differences in signal transduction in activating TSH receptor mutations in the thyroid

Author:
Kerstin Krause; Alexandra Boisnard; Christian Ihling; Marian Ludgate; Markus Eszlinger; Knut Krohn; Andrea Sinz; Dagmar Fuhrer
Source:
international journal of biochemistry & cell biology 2012 v.44 no.2 pp. 290-301
ISSN:
1357-2725
Subject:
cyclic AMP; endocytosis; functional properties; hormone receptors; mutants; mutation; phosphatidylinositol 3-kinase; phosphorylation; protein synthesis; proteomics; rats; signal transduction; thyrotropin
Abstract:
... In the thyroid, cAMP controls both thyroid growth and function. Gain-of-function mutations in the thyroid-stimulating hormone receptor (TSHR) lead to constitutive cAMP formation and are a major cause of autonomous thyroid adenomas. The impact of activating TSHR mutations on the signal transduction network of the thyrocyte is not fully understood. To gain more insights into constitutive TSHR signal ...
DOI:
10.1016/j.biocel.2011.10.024

18. Conditioned media from lung cancer cell line A549 and PC9 inactivate pulmonary fibroblasts by regulating protein phosphorylation

Author:
Ah-Mee Park; Sumio Hayakawa; Eiko Honda; Yoshihiro Mine; Koji Yoshida; Hiroshi Munakata
Source:
Archives of biochemistry and biophysics 2012 v.518 no.2 pp. 133-141
ISSN:
0003-9861
Subject:
actin; binding proteins; cAMP-dependent protein kinase; cell lines; cyclic AMP; extracellular matrix; fibroblasts; fibrosis; lung neoplasms; mechanism of action; mitogen-activated protein kinase; muscles; mutants; pathogenesis; protein phosphorylation; therapeutics; transforming growth factor beta
Abstract:
... Pulmonary fibrosis is a devastating condition resulting from excess extracellular matrix deposition that leads to progressive lung destruction and scarring. In the pathogenesis of fibrotic diseases, activation of myofibroblasts by transforming growth factor-β (TGF-β) plays a crucial role. Since no effective therapy for pulmonary fibrosis is currently recognized, finding an effective antifibrotic a ...
DOI:
10.1016/j.abb.2011.12.012

19. Cryptotanshinone from Salviae Miltiorrhizae Radix Inhibits Sodium‐nitroprusside‐induced Apoptosis in Neuro‐2a Cells

Author:
Ramalingam Mahesh; Hyo Won Jung; Gun Woo Kim; Young Shik Kim; Yong‐Ki Park
Source:
Phytotherapy research 2012 v.26 no.8 pp. 1211-1219
ISSN:
0951-418X
Subject:
cyclic AMP; reactive oxygen species; lipid peroxidation; signal transduction; adenosine monophosphate; roots; mitogen-activated protein kinase; mechanism of action; binding proteins; gene expression; herbal medicines; apoptosis; Salvia miltiorrhiza; messenger RNA; neurons; cytotoxicity; oxidants; neuroprotective effect; adenosine diphosphate; cytochrome c; membrane potential
Abstract:
... The root of Salvia miltiorrhiza (Salviae miltiorrhizae radix), a herbal medicine has widely been used for the treatment of pain, miscarriage and oedema. In this study, we evaluated the neuroprotective effect of cryptotanshinone (CRT) from Salviae miltiorrhizae radix on sodium‐nitroprusside (SNP)‐induced apoptosis in neuro‐2a (N2a) cells, and further investigated its action mechanism in signalling ...
DOI:
10.1002/ptr.3705
PubMed:
22228596

20. Curing viruses in Pleurotus ostreatus by growth on a limited nutrient medium containing cAMP and rifamycin

Author:
Y.C. Kwon; D.W. Jeong; S.I. Gim; H.S. Ro; H.S. Lee
Source:
Journal of virological methods 2012 v.185 no.1 pp. 156-159
ISSN:
0166-0934
Subject:
Pleurotus ostreatus; cyclic AMP; enzyme-linked immunosorbent assay; fruiting bodies; mushroom compost; mushrooms; mycelium; mycoviruses; reverse transcriptase polymerase chain reaction; virus curing
Abstract:
... Oyster mushroom spherical virus (OMSV) and oyster mushroom isometric virus (OMIV) are the causative agents of a fruiting body deformation disease in the edible mushroom Pleurotus ostreatus. The curing of these mycoviruses was facilitated by a serial transfer of infected mycelia onto a limited nutrient medium containing 1mM of cAMP and 75μg/ml of rifamycin (cAMP-rifamycin plate). The mycelia were g ...
DOI:
10.1016/j.jviromet.2012.06.002

21. Cyclic AMP and Cyclic AMP-receptor Protein Modulate the Autoinducer-2-Mediated Quorum Sensing System in Vibrio vulnificus

Author:
Sun-Pyo Kim; Choon-Mee Kim; Sung-Heui Shin
Source:
Current microbiology 2012 v.65 no.6 pp. 701-710
ISSN:
0343-8651
Subject:
Vibrio harveyi; Vibrio vulnificus; adenosine monophosphate; adenylate cyclase; cyclic AMP; glucose; quorum sensing
Abstract:
... This study was undertaken to determine whether cyclic AMP (cAMP) or cAMP-receptor protein (CRP) modulates the activity of the autoinducer (AI)-2-mediated quorum sensing (QS) system in response to glucose availability in Vibrio vulnificus. A mutation in crp impaired V. vulnificus growth, decreased AI-2 production, and repressed the expression of smcR encoding the master regulator SmcR (a Vibrio har ...
DOI:
10.1007/s00284-012-0218-0
PubMed:
22961036

22. Cyclic AMP-receptor protein activates aerobactin receptor IutA expression in Vibrio vulnificus

Author:
Choon-Mee Kim; Seong-Jung Kim; Sung-Heui Shin
Source:
journal of microbiology 2012 v.50 no.2 pp. 320-325
ISSN:
1225-8873
Subject:
Escherichia coli; Vibrio vulnificus; adenosine monophosphate; adenylate cyclase; bacteria; cyclic AMP; environmental factors; glucose; mutation; siderophores
Abstract:
... The ferrophilic bacterium Vibrio vulnificus can utilize the siderophore aerobactin of Escherichia coli for iron acquisition via its specific receptor IutA. This siderophore piracy by V. vulnificus may contribute to its survival and proliferation, especially in mixed bacterial environments. In this study, we examined the effects of glucose, cyclic AMP (cAMP), and cAMP-receptor protein (Crp) on iutA ...
DOI:
10.1007/s12275-012-2056-y

23. Determination of cyclic guanosine- and cyclic adenosine monophosphate (cGMP and cAMP) in human plasma and animal tissues by solid phase extraction on silica and liquid chromatography–triple quadrupole mass spectrometry

Author:
Thomas Van Damme; Yanhua Zhang; Frédéric Lynen; Pat Sandra
Source:
Journal of chromatography 2012 v.909 no. pp. 14-21
ISSN:
1570-0232
Subject:
animal tissues; cell growth; cyclic AMP; cyclic GMP; guanosine monophosphate; humans; hydrophilicity; isomers; mass spectrometry; metabolites; patients; reversed-phase liquid chromatography; signal transduction; silica; solid phase extraction
Abstract:
... 3′,5′-Cyclic guanosine monophosphate (cGMP) and 3′,5′-cyclic adenosine monophosphate (cAMP) are essential second messenger molecules. They are involved in signal transduction within cells, in physiological functions such as neurotransmission and in the modulation of cell growth and differentiation of organisms, respectively. A quantitative solid phase extraction method (SPE) based on hydrophilic i ...
DOI:
10.1016/j.jchromb.2012.10.002

24. Development of an Environmentally Friendly Adsorbent for the Removal of Toxic Heavy Metals from Aqueous Solution

Author:
Jae-Woo Choi; Seung-Gun Chung; Seok-Won Hong; Dong-Ju Kim; Sang-Hyup Lee
Source:
Water, air, and soil pollution 2012 v.223 no.4 pp. 1837-1846
ISSN:
0049-6979
Subject:
Ziziphus; adsorbents; adsorption; aqueous solutions; batch systems; casein; cyclic AMP; drying; heavy metals; models; powders; surface area; toxicity; wastewater
Abstract:
... An effective adsorbent for the removal of heavy metals was manufactured by immobilization of jujube powder. The adsorptions of Cd, Zn and Cu from aqueous solutions by jujube complex beads (Type 1 and Type 2) were studied in a batch adsorption system. The adsorption data were fitted well with the Langmuir isotherm models. The adsorption capacities (β) for Cd, Zn and Cu were 4.23, 2.93 and 3.64 mg/g ...
DOI:
10.1007/s11270-011-0988-1

25. Differential expression of cyclic-AMP responsive element modulator (CREM) mRNA isoforms during testicular development of boars

Author:
B.L. Sartini; M. Eerhart; S. Green
Source:
Theriogenology 2012 v.77 no.5 pp. 881-887
ISSN:
0093-691X
Subject:
alternative splicing; boars; cyclic AMP; exons; messenger RNA; rodents; spermatogenesis; testes; testicular development; transcription factors
Abstract:
... The cyclic-AMP responsive element modulator (CREM) transcription factor, a key regulator of spermatogenesis, can activate or repress gene expression by differential expression of alternatively spliced mRNA isoforms. The objective of this study was to report the sequences of the predominant CREM mRNA isoforms with leader exons B, θ1, θ2, in addition to the relative expression of these transcripts, ...
DOI:
10.1016/j.theriogenology.2011.09.013
PubMed:
22056015

26. Direct Peptide Bioconjugation/PEGylation at Tyrosine with Linear and Branched Polymeric Diazonium Salts

Author:
Jones Mathew W.; Mantovani Giuseppe; Blindauer Claudia A.; Ryan Sinead M.; Wang Xuexuan; Brayden David J.; Haddleton David M.
Source:
Journal of the American Chemical Society 2012 v.134 no.17 pp. 7406-7413
ISSN:
1520-5126
Subject:
animal models; breast neoplasms; calcitonin; calcium; chemical bonding; cyclic AMP; enkephalin; ethylene glycol; humans; in vitro studies; leucine; neoplasm cells; polyethylene glycol; polymerization; rats; receptors; salmon; salts; tyrosine
Abstract:
... Direct polymer conjugation at peptide tyrosine residues is described. In this study Tyr residues of both leucine enkephalin and salmon calcitonin (sCT) were targeted using appropriate diazonium salt-terminated linear monomethoxy poly(ethylene glycol)s (mPEGs) and poly(mPEG) methacrylate prepared by atom transfer radical polymerization. Judicious choice of the reaction conditionspH, stoichiometry, ...
DOI:
10.1021/ja211855q

27. Discovery of antagonists of tick dopamine receptors via chemical library screening and comparative pharmacological analyses

Author:
Karin F.K. Ejendal; Jason M. Meyer; Tarsis F. Brust; Larisa V. Avramova; Catherine A. Hill; Val J. Watts
Source:
Insect biochemistry and molecular biology 2012 v.42 no.11 pp. 846-853
ISSN:
0965-1748
Subject:
Ixodes scapularis; Lyme disease; dopamine; neurophysiology; ticks; insecticides; humans; antagonists; cyclic AMP; dopamine receptors; luciferase; Aedes aegypti; vector control; acaricides; screening; pathogens
Abstract:
... Ticks transmit a wide variety of disease causing pathogens to humans and animals. Considering the global health impact of tick-borne diseases, there is a pressing need to develop new methods for vector control. We are exploring arthropod dopamine receptors as novel targets for insecticide/acaricide development because of their integral roles in neurobiology. Herein, we developed a screening assay ...
DOI:
10.1016/j.ibmb.2012.07.011
PubMed:
23213654

28. Dual Inhibition of γ-Oryzanol on Cellular Melanogenesis: Inhibition of Tyrosinase Activity and Reduction of Melanogenic Gene Expression by a Protein Kinase A-Dependent Mechanism

Author:
Jun Hee-jin; Lee Ji Hae; Cho Bo-Ram; Seo Woo-Duck; Kang Hang-Won; Kim Dong-Woo; Cho Kang-Jin; Lee Sung-Joon
Source:
Journal of natural products 2012 v.75 no.10 pp. 1706-1711
ISSN:
1520-6025
Subject:
binding proteins; cAMP-dependent protein kinase; cyclic AMP; enzyme activity; gene expression; gene expression regulation; melanin; melanogenesis; messenger RNA; oryzanol; phosphorylation; phytosterols; rice; transcription (genetics); transcription factors; transcriptomics
Abstract:
... The in vitro effects on melanogenesis of γ-oryzanol (1), a rice bran-derived phytosterol, were investigated. The melanin content in B16F1 cells was significantly and dose-dependently reduced (−13% and −28% at 3 and 30 μM, respectively). Tyrosinase enzyme activity was inhibited by 1 both in a cell-free assay and when analyzed based on the measurement of cellular tyrosinase activity. Transcriptome a ...
DOI:
10.1021/np300250m

29. EGF-like peptide of Dictyostelium discoideum is not a chemoattractant but it does restore folate-mediated chemotaxis in the presence of signal transduction inhibitors

Author:
Ina Nikolaeva; Robert J. Huber; Danton H. O’Day
Source:
Peptides 2012 v.34 no.1 pp. 145-149
ISSN:
0196-9781
Subject:
Dictyostelium discoideum; bioassays; calmodulin; cell movement; chemoattractants; chemotaxis; cyclic AMP; extracellular matrix; folic acid; migratory behavior; peptides; phosphatidylinositol 3-kinase; phospholipase A2; signal transduction; tyrosine; vegetative cells
Abstract:
... A synthetic EGF-like (EGFL) peptide (DdEGFL1), equivalent to the first EGFL domain in the extracellular matrix protein CyrA, has previously been shown to enhance random cell motility and cAMP-mediated chemotaxis in Dictyostelium discoideum. However the role of DdEGFL1 as a potential chemoattractant had not been addressed. In this study, a micropipette assay and an under-agarose migration assay sho ...
DOI:
10.1016/j.peptides.2011.12.014

30. Effect of vitamin D₃ in reducing metabolic and oxidative stress in the liver of streptozotocin-induced diabetic rats

Author:
Naijil George; T. Peeyush Kumar; Sherin Antony; S. Jayanarayanan; C. S. Paulose
Source:
British journal of nutrition 2012 v.108 no.8 pp. 1410-1418
ISSN:
1475-2662
Subject:
adults; animal disease models; carbon; cholecalciferol; cyclic AMP; cyclic GMP; diabetes mellitus; free radicals; gene expression; glucose; glutathione peroxidase; glycogen; inositols; insulin; insulin receptors; liver; malate dehydrogenase; males; metabolism; oxidative stress; phospholipase C; quantitative polymerase chain reaction; radionuclides; rats; second messengers; streptozotocin; superoxide dismutase
Abstract:
... Diabetes mellitus is a growing health problem worldwide and is associated with severe liver complications. The aim of the present study is to analyse the status of metabolic and free-radical-scavenging enzymes and second messengers in the liver of streptozotocin (STZ)-induced diabetic rats, and to determine the hepatoprotective role of vitamin D₃. All studies were performed using the liver of adul ...
DOI:
10.1017/S0007114511006830
PubMed:
22221397

31. Endothelium dependent expression and underlying mechanisms of des-Arg⁹-bradykinin-induced B₁R-mediated vasoconstriction in rat portal vein

Author:
Fernanda L. Basei; Daniela A. Cabrini; Cláudia P. Figueiredo; Stefânia Forner; Daniela B. Hara; Andrey F.Z. Nascimento; Graziela S. Ceravolo; Maria Helena C. Carvalho; Michael Bader; Rodrigo Medeiros; João B. Calixto
Source:
Peptides 2012 v.37 no.2 pp. 216-224
ISSN:
0196-9781
Subject:
antagonists; bradykinin; cyclic AMP; endothelium; myocytes; phosphatidylinositol 3-kinase; portal hypertension; portal vein; prostaglandin synthase; protein kinase C; rats; receptors; smooth muscle; transcription factors; vasoconstriction
Abstract:
... Endothelial dysfunction has been implicated in portal vein obstruction, a condition responsible for major complications in chronic portal hypertension. Increased vascular tone due to disruption of endothelial function has been associated with an imbalance in the equilibrium between endothelium-derived relaxing and contracting factors. Herein, we assessed underlying mechanisms by which expression o ...
DOI:
10.1016/j.peptides.2012.07.020

32. Engineering global transcription factor cyclic AMP receptor protein of Escherichia coli for improved 1-butanol tolerance

Author:
Hongfang Zhang; Huiqing Chong; Chi Bun Ching; Hao Song; Rongrong Jiang
Source:
Applied microbiology and biotechnology 2012 v.94 no.4 pp. 1107-1117
ISSN:
0175-7598
Subject:
engineering; cyclic AMP; adenosine monophosphate; scanning electron microscopy; genes; hydrophobicity; receptors; fermentation; screening; gene expression regulation; Escherichia coli; transcription factors; DNA libraries; hosts; mutagenesis; adhesion; hydrocarbons; stress response; reverse transcriptase polymerase chain reaction; biofuels; mutants
Abstract:
... One major challenge in biofuel production, including biobutanol production, is the low tolerance of the microbial host towards increasing biofuel concentration during fermentation. Here, we have demonstrated that Escherichia coli 1-butanol tolerance can be greatly enhanced through random mutagenesis of global transcription factor cyclic AMP receptor protein (CRP). Four mutants (MT1–MT4) with eleva ...
DOI:
10.1007/s00253-012-4012-5
PubMed:
22466954

33. Environmental Contaminant Mixtures at Ambient Concentrations Invoke a Metabolic Stress Response in Goldfish Not Predicted from Exposure to Individual Compounds Alone

Author:
Jordan Julia; Zare Ava; Jackson Leland J.; Habibi Hamid R.; Weljie Aalim M.
Source:
Journal of Proteome Research 2012 v.11 no.2 pp. 1133-1143
ISSN:
1535-3907
Subject:
adverse effects; bioinformatics; bisphenol A; cyclic AMP; energy; genomics; goldfish; lipid metabolism; liver; metabolomics; models; nuclear magnetic resonance spectroscopy; phenotype; pollutants; proteome; reproduction; risk assessment; spectral analysis; stress response; testes; transcriptomics
Abstract:
... Environmental contaminants from wastewater and industrial or agricultural areas are known to have adverse effects on development, reproduction, and metabolism. However, reliable assessment of environmental contaminant impact at low (i.e., ambient) concentrations using genomics and transcriptomics approaches has proven challenging. A goldfish model was used to investigate the effects of aquatic pol ...
DOI:
10.1021/pr200840b

34. Error-prone PCR of global transcription factor cyclic AMP receptor protein for enhanced organic solvent (toluene) tolerance

Author:
Souvik Basak; Hao Song; Rongrong Jiang
Source:
Process biochemistry 2012 v.47 no.12 pp. 2152-2158
ISSN:
1359-5113
Subject:
Escherichia coli; adenosine monophosphate; amino acids; biocatalysis; bioremediation; cyclic AMP; cyclohexanes; engineering; genes; hexane; microorganisms; mutagenesis; mutants; reverse transcriptase polymerase chain reaction; scanning electron microscopy; solvents; toluene; transcription factors; xylene
Abstract:
... Organic solvents are often applied in biphasic biocatalysis or involved in bioremediation, but native microorganisms usually have low tolerance toward these organic solvents and cannot survive in the stressful environment. Here, we aimed to improve the organic solvent tolerance of Escherichia coli by engineering its global transcription factor cAMP receptor protein (CRP). Toluene was chosen as mod ...
DOI:
10.1016/j.procbio.2012.08.006

35. Evidence for tubular mating structures induced in each mating type of heterothallic gonium pectorale (volvocales, chlorophyta)1

Author:
Yuko Mogi; Takashi Hamaji; Masahiro Suzuki; Patrick Ferris; Toshiyuki Mori; Yukihiro Kabeya; Shin‐ya Miyagishima; Hisayoshi Nozaki
Source:
Journal of phycology 2012 v.48 no.3 pp. 670-674
ISSN:
0022-3646
Subject:
Chlamydomonas reinhardtii; Gonium; actin; cell walls; cyclic AMP; germ cells
Abstract:
... Gametes were induced separately in cultures of each mating type of the heterothallic, isogamous colonial volvocalean Gonium pectorale O. F. Müll. to examine the tubular mating structure (TMS) of both mating types plus and minus (plus and minus), referred to as “bilateral mating papillae.” Addition of dibutyryl cyclic adenosine monophosphate (DcAMP or db‐cAMP) and 3‐isobutyl‐1‐methylxanthine (IBMX) ...
DOI:
10.1111/j.1529-8817.2012.01149.x

36. Exchange protein activated by cyclic adenosine monophosphate regulates the switch between adipogenesis and osteogenesis of human mesenchymal stem cells through increasing the activation of phosphatidylinositol 3-kinase

Author:
Zihua Tang; Dongyan Shi; Bingbing Jia; Jiarong Chen; Chen Zong; Dan Shen; Qiang Zheng; Jinfu Wang; Xiangming Tong
Source:
international journal of biochemistry & cell biology 2012 v.44 no.7 pp. 1106-1120
ISSN:
1357-2725
Subject:
adipogenesis; binding proteins; bone formation; cyclic AMP; gene expression; gene expression regulation; genes; humans; mitogen-activated protein kinase; non-specific protein-tyrosine kinase; osteoporosis; phosphatidylinositol 3-kinase; small interfering RNA; stem cells; transcription factors
Abstract:
... Epac, exchange protein activated by cyclic adenosine monophosphate (cAMP), could regulate the trans-differentiation between adipogenesis and osteogenesis of human mesenchymal stem cells (hMSCs). Epac activated by 8-pCPT-2′-O-Me-cAMP, a cAMP analog preferentially activating Epac, resulted in the increase of adipogenic gene expression and the decrease of osteogenic gene expression. The pro-adipogeni ...
DOI:
10.1016/j.biocel.2012.03.019

37. Exposure of Sebastiscus marmoratus embryos to pyrene results in neurodevelopmental defects and disturbs related mechanisms

Author:
Chengyong He; Chonggang Wang; Bowen Li; Meifang Wu; Hong Geng; Yixin Chen; Zhenghong Zuo
Source:
Aquatic toxicology 2012 v.116-117 no. pp. 109-115
ISSN:
0166-445X
Subject:
pyrene (hydrocarbon); neurotoxicity; central nervous system; protein synthesis; motor neurons; nitric oxide; acetylcholine; calcium; cyclic AMP; environmental exposure; innervation; pollution; axons; cardiovascular system; phosphotransferases (kinases); neurotransmitters; death; receptors; calmodulin
Abstract:
... Polycyclic aromatic hydrocarbons (PAHs) are widespread environmental contaminants, which are known to be carcinogenic and teratogenic. These compounds cause a range of macroscopic malformations, particularly to the craniofacial apparatus and cardiovascular system during vertebrate development. However, little is known concerning microscopic effects, especially on the sensitive early life stages or ...
DOI:
10.1016/j.aquatox.2012.03.009
PubMed:
22487263

38. Extracellular calcium is involved in egg yolk-induced head-to-head agglutination of bull sperm

Author:
D.H. Yang; A.G. McMillan; N.T. Standley; P. Shannon; Z.Z. Xu
Source:
Theriogenology 2012 v.78 no.7 pp. 1476-1486
ISSN:
0093-691X
Subject:
agglutination; calcium; calcium chloride; cyclic AMP; egg yolk; eggs; membrane potential; plasma membrane; protein kinases; semen; spermatozoa; streptomycin
Abstract:
... Head-to-head agglutination of bull sperm occurs when semen is highly diluted in an egg yolk-citrate diluent without streptomycin. The objectives were to investigate causes of sperm agglutination and the underlying mechanism. Aliquots of bull semen were diluted in a base diluent (BD) supplemented with various test components and the percentage of agglutinated sperm (% AggSp) was quantified at 1, 5, ...
DOI:
10.1016/j.theriogenology.2012.06.021
PubMed:
22925643

39. Factors governing the regulation of Sclerotinia sclerotiorum cutinase A and polygalacturonase 1 during different stages of infection

Author:
Zafer Dallal Bashi; S. Roger Rimmer; George G. Khachatourians; Dwayne D. Hegedus
Source:
Canadian journal of microbiology 2012 v.58 no.5 pp. 605-616
ISSN:
1480-3275
Subject:
Sclerotinia sclerotiorum; calcium signaling; cell walls; cutinase; cyclic AMP; epicuticular wax; galacturonic acid; gene expression; genes; glucose; leaves; mycelium; plant cuticle; polygalacturonase; starvation; virulence
Abstract:
... Sclerotinia sclerotiorum releases hydrolytic enzymes that sequentially degrade the plant cuticle, middle lamellae, and primary and secondary cell walls. The cuticle was found to be a barrier to S. sclerotiorum infection, as leaves stripped of epicuticular wax were more rapidly colonized. Consequently, the factors affecting the regulation of genes encoding polygalacturonase 1 (SsPG1) and a newly id ...
DOI:
10.1139/w2012-031

40. Forced expression of stabilized c-Fos in dendritic cells reduces cytokine production and immune responses in vivo

Author:
Ryoko Yoshida; Mayu Suzuki; Ryota Sakaguchi; Eiichi Hasegawa; Akihiro Kimura; Takashi Shichita; Takashi Sekiya; Hiroshi Shiraishi; Kouji Shimoda; Akihiko Yoshimura
Source:
Biochemical and biophysical research communications 2012 v.423 no.2 pp. 247-252
ISSN:
0006-291X
Subject:
T-lymphocytes; adaptive immunity; cell proliferation; coculture; cyclic AMP; dendritic cells; encephalitis; genetically modified organisms; half life; immune response; innate immunity; interleukin-10; interleukin-12; interleukin-23; mice; mutation; phosphorylation; transcription factors; tumor necrosis factor-alpha
Abstract:
... Intracellular cyclic adenosine monophosphate (cAMP) suppresses innate immunity by inhibiting proinflammatory cytokine production by monocytic cells. We have shown that the transcription factor c-Fos is responsible for cAMP-mediated suppression of inflammatory cytokine production, and that c-Fos protein is stabilized by IKKβ-mediated phosphorylation. We found that S308 is one of the major phosphory ...
DOI:
10.1016/j.bbrc.2012.05.097

41. Functional status and relationships of melanocortin 1 receptor signaling to the cAMP and extracellular signal-regulated protein kinases 1 and 2 pathways in human melanoma cells

Author:
Cecilia Herraiz; Fabrice Journé; Ghanem Ghanem; Celia Jiménez-Cervantes; José C. García-Borrón
Source:
international journal of biochemistry & cell biology 2012 v.44 no.12 pp. 2244-2252
ISSN:
1357-2725
Subject:
adenylate cyclase; agonists; alpha-melanocyte-stimulating hormone; cyclic AMP; forskolin; functional status; genotype; humans; melanocytes; melanoma; mitogen-activated protein kinase; mutation; transcriptional activation
Abstract:
... Melanocortin 1 receptor (MC1R), a major determinant of skin phototype frequently mutated in melanoma, is a Gs protein-coupled receptor that regulates pigment production in melanocytes. MC1R stimulation activates cAMP synthesis and the extracellular signal-regulated (ERK) ERK1 and ERK2. In human melanocytes, ERK activation by MC1R relies on cAMP-independent transactivation of the c-KIT receptor. Th ...
DOI:
10.1016/j.biocel.2012.09.008

42. Gene cloning and function analysis of complement B factor-2 of Apostichopus japonicus

Author:
Lei Zhong; Feng Zhang; Yaqing Chang
Source:
Fish & shellfish immunology 2012 v.33 no.3 pp. 504-513
ISSN:
1050-4648
Subject:
Apostichopus japonicus; Carcinoscorpius; Strongylocentrotus; amino acid sequences; amino acids; binding sites; bioinformatics; blood coagulation factors; cGMP-dependent protein kinase; complement; complementary DNA; cyclic AMP; gene expression; genes; glycosylation; integument; invertebrates; magnesium; molecular cloning; molecular weight; open reading frames; phosphorylation; phylogeny; reverse transcriptase polymerase chain reaction; serine proteinases; vertebrates
Abstract:
... In this study, a homologue of complement B factor (AjBf-2, GenBank ID: JN634069.1) was cloned and characterized from Apostichopus japonicus by using bioinformatics methods and molecular biotechnologies including homology cloning and RACE. The full-length cDNA of AjBf-2 was composed of 3261bp. The sequence shows 268bp in the 5′UT region, 395bp in the 3′UT region, and 2595 bp in the open reading fra ...
DOI:
10.1016/j.fsi.2012.06.001

43. Helicity Control of an Indolocarbazole Foldamer by Chiral Organic Anions

Author:
Suk Jae-min; Kim Dan A; Jeong Kyu-Sung
Source:
Organic letters 2012 v.14 no.19 pp. 5018-5021
ISSN:
1523-7052
Subject:
anions; chemical reactions; chemical structure; cyclic AMP; organic compounds
Abstract:
... Chiral organic anions such as camphorsulfonates and cAMP give rise to the preferential formation of a one-handed helix of an indolocarbazole foldamer, thus inducing characteristic circular dichroic (CD) signals. Moreover, the on and off switching of the chiroptical signal can be operated by acid and base chemistry which efficiently controls the association and dissipation of the foldamer and cAMP. ...
DOI:
10.1021/ol3022148

44. IP₃-dependent intracellular Ca²⁺ release is required for cAMP-induced c-fos expression in hippocampal neurons

Author:
Wenting Zhang; Asmita Tingare; David Chi-Heng Ng; Hong W. Johnson; Michael J. Schell; Rebecca L. Lord; Sangeeta Chawla
Source:
Biochemical and biophysical research communications 2012 v.425 no.2 pp. 450-455
ISSN:
0006-291X
Subject:
DNA; calcium; cyclic AMP; gene expression; luciferase; neurons; reporter genes; synapse; transcription factors
Abstract:
... Ca²⁺ and cAMP are widely used in concert by neurons to relay signals from the synapse to the nucleus, where synaptic activity modulates gene expression required for synaptic plasticity. Neurons utilize different transcriptional regulators to integrate information encoded in the spatiotemporal dynamics and magnitude of Ca²⁺ and cAMP signals, including some that are Ca²⁺-responsive, some that are cA ...
DOI:
10.1016/j.bbrc.2012.07.122

45. Involvement of hippocampal CAMKII/CREB signaling in the spatial memory retention induced by creatine

Author:
Mauren Assis Souza; Danieli Valnes Magni; Gustavo Petri Guerra; Mauro Schneider Oliveira; Ana Flávia Furian; Letícia Pereira; Silvia Vacari Marquez; Juliano Ferreira; Michele Rechia Fighera; Luiz Fernando Freire Royes
Source:
Amino acids 2012 v.43 no.6 pp. 2491-2503
ISSN:
0939-4451
Subject:
binding proteins; cAMP-dependent protein kinase; creatine; cyclic AMP; energy; hippocampus; memory; neurons; phosphocreatine; statistical analysis
Abstract:
... Although Creatine (Cr) and Phosphocreatine (PCr) systems play a key role in cellular energy and energy transport in neuronal cells, its implications for learning and memory are still controversial. Thus, we decided to investigate the involvement of cAMP-dependent protein kinase A (PKA), Ca²⁺/calmodulin-dependent protein kinase II (CaMKII) and cAMP responsive element binding protein (CREB) in the s ...
DOI:
10.1007/s00726-012-1329-4

46. Involvements of calcium channel and potassium channel in Danshen and Gegen decoction induced vasodilation in porcine coronary LAD artery

Author:
Fan Hu; Chi Man Koon; Judy Yuet Wa Chan; Kit Man Lau; Y.W. Kwan; Kwok Pui Fung
Source:
Phytomedicine 2012 v.19 no.12 pp. 1051-1058
ISSN:
0944-7113
Subject:
Salvia miltiorrhiza; calcium; calcium channels; calcium chloride; coronary vessels; cyclic AMP; cyclic GMP; endothelium; herbal medicines; hypertension; myosin light chains; patients; potassium; potassium channels; swine; vasodilation
Abstract:
... Danshen (Salviae Miltiorrhizae Radix) and Gegen (Puerariae Lobatae Radix) have been widely used in treating cardiovascular diseases for thousands of years in China. The present study was carried out to evaluate the effects of a Danshen and Gegen decoction (DG) on the vascular reactivity of a porcine isolated coronary artery and the underlying mechanisms involved. Porcine coronary rings were precon ...
DOI:
10.1016/j.phymed.2012.07.007

47. Isodesacetyluvaricin, an Annonaceous Acetogenin, Specifically Inhibits Gene Expression of Cyclooxygenase-2

Author:
Wu Tung-Ying; Yang I-Hui; Tsai Yao-Ting; Wang Jaw-Yan; Shiurba Robert; Hsieh Tusty-Jiuan; Chang Fang-Rong; Chang Wei-Chiao
Source:
Journal of natural products 2012 v.75 no.4 pp. 572-576
ISSN:
1520-6025
Subject:
Annona glabra; T-lymphocytes; Western blotting; acetogenins; angiogenesis; anti-inflammatory activity; calcium; cell proliferation; colorimetry; cyclic AMP; fluorescence; fruit trees; humans; image analysis; luciferase; messenger RNA; metastasis; mice; neoplasm cells; neoplasms; phytopharmaceuticals; prostaglandin synthase; reverse transcriptase polymerase chain reaction; transcription (genetics)
Abstract:
... Cyclooxygenase-2 (COX-2) is an inducible isoform of the enzyme responsible for the synthesis of several inflammatory mediators. In a search for phytochemicals with anti-inflammatory activity, the COX-2 inhibitory activity of 15 typical Annonaceous acetogenins was examined. Isodesacetyluvaricin (1), from the Formosan tropical fruit tree Annona glabra, exhibited the most potent activity. Reverse tra ...
DOI:
10.1021/np200719r

48. Lancemaside A isolated from Codonopsis lanceolata and its metabolite echinocystic acid ameliorate scopolamine-induced memory and learning deficits in mice

Author:
Il-Hoon Jung; Se-Eun Jang; Eun-Ha Joh; Jayong Chung; Myung Joo Han; Dong-Hyun Kim
Source:
Phytomedicine 2012 v.20 no.1 pp. 84-88
ISSN:
0944-7113
Subject:
Codonopsis lanceolata; acetylcholinesterase; binding proteins; blood; cyclic AMP; dose response; herbal medicines; inflammation; inhibitory concentration 50; intestinal microorganisms; memory; metabolites; mice; oral administration; rhizomes; scopolamine; sleep disorders
Abstract:
... The rhizome of Codonopsis lanceolata (family Campanulaceae), which contains lancemaside A as a main constituent, has been used as herbal medicine to treat inflammation, insomnia, and hypomnesia. Lancemaside A and echinocystic acid, which is its metabolite by intestinal microflora, potently inhibited acetylcholinesterase activity in a dose-dependent manner, with IC₅₀ value 13.6μM and 12.2μM, respec ...
DOI:
10.1016/j.phymed.2012.09.005

49. Linalool from Rosewood (Aniba rosaeodora Ducke) Oil Inhibits Adenylate Cyclase in the Retina, Contributing to Understanding its Biological Activity

Author:
Lucia de Fatima S. Sampaio; José Guilherme S. Maia; Amanda M. Parijós; Rita Z. Souza; Lauro Euclides S. Barata
Source:
Phytotherapy research 2012 v.26 no.1 pp. 73-77
ISSN:
0951-418X
Subject:
Aniba; adenylate cyclase; bioactive properties; biochemical mechanisms; central nervous system; chicks; cyclic AMP; enzyme inhibition; essential oils; forskolin; linalool; retina; signal transduction
Abstract:
... Rosewood oil (RO) (Aniba rosaeodora Ducke) is rich in linalool, a monoterpene alcohol, which has well studied anxiolytic, sedative and anticonvulsant effects. The inhibition of the increases in cAMP protects against seizures in a diversity of models of epilepsy. In this paper, the principal aim was to investigate the effects of RO, (±)‐linalool and (‐)‐linalool) on adenylate cyclase. They were tes ...
DOI:
10.1002/ptr.3518
PubMed:
21544884

50. Lymphopenia induced by a novel selective S1P₁ antagonist structurally unrelated to S1P

Author:
Yasuyuki Fujii; Hidenori Ohtake; Naoya Ono; Toshifumi Hara; Takanobu Sakurai; Syuya Takahashi; Tetsuo Takayama; Yoshiki Fukasawa; Fumiyasu Shiozawa; Narutoshi Tsukahara; Takehiro Hirayama; Yasuyuki Igarashi; Ryo Goitsuka
Source:
Biochimica et biophysica acta 2012 v.1821 no.4 pp. 600-606
ISSN:
1388-1981
Subject:
agonists; antagonists; chemotaxis; cyclic AMP; ears; hyperplasia; hypersensitivity; leukocytes; mice; models; sphingosine
Abstract:
... Sphingosine 1-phosphate (S1P) regulates lymphocyte trafficking via type-1 S1P receptor (S1P₁) and participates in many pathological conditions. We developed a novel type S1P₁-selective antagonist, TASP0251078, which is structurally unrelated to S1P. This competitive antagonist inhibited binding of S1P to S1P₁ resulting in reduced signaling downstream of S1P₁, including GTPγS-binding and cAMP forma ...
DOI:
10.1016/j.bbalip.2011.12.006