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1. Bovine intramammary Escherichia coli challenge infections in late gestation demonstrate a dominant antiinflammatory immunological response

Author:
R.R. Quesnell; S. Klaessig; J.L. Watts; Y.H. Schukken
Source:
Journal of dairy science 2012 v.95 no.1 pp. 117-126
ISSN:
0022-0302
Subject:
DNA; Escherichia coli; coliform bacteria; colostrum; cows; death; early lactation; immune response; inflammation; interleukin-10; interleukin-1beta; mammary glands; mastitis; milk; milk production; parturition; pregnancy; somatic cell count; tumor necrosis factor-alpha
Abstract:
... Coliform mastitis that presents itself at parturition or in the early weeks of bovine lactation is often characterized by severe inflammation and impaired milk production and can lead to death of the animal. Chronic intramammary infections caused by persistent strains of Escherichia coli may result in high production losses. The aim of this study was to determine the inflammatory response to a tea ...
DOI:
10.3168/jds.2011-4289
PubMed:
22192191

2. Canine babesiosis in Romania due to Babesia canis and Babesia vogeli: a molecular approach

Author:
Mariana Ionita; Ioan Liviu Mitrea; Kurt Pfister; Dietmar Hamel; Catalin Marius Buzatu; Cornelia Silaghi
Source:
Parasitology research 2012 v.110 no.5 pp. 1659-1664
ISSN:
0932-0113
Subject:
Babesia canis; DNA; babesiosis; blood; blood sampling; death; dogs; genes; hematologic tests; polymerase chain reaction; ribosomal RNA; sequence analysis; surveys; Romania
Abstract:
... Canine babesiosis is a tick-borne disease caused by the protozoa Babesia spp. that affects dogs worldwide. In Romania, canine babesiosis has become quite frequent in the last few years, with a wide variety of clinical signs, ranging from mild, nonspecific illness to peracute collapse, and even death. Traditionally, a Babesia infection in dogs is diagnosed based on the morphologic appearance of the ...
DOI:
10.1007/s00436-011-2683-y
PubMed:
22006189

3. DNase activation by hypoxia–acidosis parallels but is independent of programmed cell death

Author:
John W. Thompson; Regina M. Graham; Keith A. Webster
Source:
Life sciences 2012 v.91 no.7-8 pp. 223-229
ISSN:
0024-3205
Subject:
DNA; DNA fragmentation; acidosis; apoptosis; calpain; cardiomyocytes; caspases; death; deoxyribonucleases; hypoxia; lysosomes; membrane potential; mitochondria; mitochondrial membrane; myocardial infarction; necrosis; normoxia; pH; permeability; rats
Abstract:
... AIMS: Hypoxia, acidosis and programmed cell death are each hallmarks of acute myocardial infarction (AMI). We previously described a death pathway of cardiac myocytes mediated by hypoxia–acidosis that was characterized by activation of the Bcl2-family protein Bnip3 and programmed necrosis. The pathway included extensive DNA fragmentation that was sensitive to inhibition of the mitochondrial permea ...
DOI:
10.1016/j.lfs.2012.03.034
PubMed:
22525374
PubMed Central:
PMC3638004

4. Detection of mRNA by reverse‐transcription PCR as an indicator of viability in Phytophthora ramorum

Author:
A. Chimento; S. O. Cacciola; M. Garbelotto
Source:
Forest pathology 2012 v.42 no.1 pp. 14-21
ISSN:
1437-4781
Subject:
DNA; Laurus nobilis; Phytophthora ramorum; Quercus; blight; cytochrome-c oxidase; death; disease diagnosis; freeze drying; genes; leaves; messenger RNA; plant diseases and disorders; plant pathogens; polymerase chain reaction; selective media; viability
Abstract:
... In the last few decades, the use of molecular tools has greatly improved the efficiency of plant disease diagnosis. However, one of the major setbacks of most molecular diagnostic approaches is their inability to differentiate between dead and viable pathogens. We propose a new strategy for the detection of plant pathogens, based on the use of mRNA as a viability marker, on the basis that mRNA deg ...
DOI:
10.1111/j.1439-0329.2011.00717.x

5. Endonuclease VIII-like 1 (NEIL1) promotes short-term spatial memory retention and protects from ischemic stroke-induced brain dysfunction and death in mice

Author:
Chandrika Canugovi; Jeong Seon Yoon; Neil H. Feldman; Deborah L. Croteau; Mark P. Mattson; Vilhelm A. Bohr
Source:
Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.37 pp. 14948-14953
ISSN:
0027-8424
Subject:
DNA; DNA damage; DNA repair; anxiety; brain; brain damage; death; fearfulness; glutamic acid; glycosylases; ischemia; memory; mice; models; neurons; receptors; stroke
Abstract:
... Recent findings suggest that neurons can efficiently repair oxidatively damaged DNA, and that both DNA damage and repair are enhanced by activation of excitatory glutamate receptors. However, in pathological conditions such as ischemic stroke, excessive DNA damage can trigger the death of neurons. Oxidative DNA damage is mainly repaired by base excision repair (BER), a process initiated by DNA gly ...
DOI:
10.1073/pnas.1204156109
PubMed:
22927410
PubMed Central:
PMC3443144

6. Engineered Repressible Lethality for Controlling the Pink Bollworm, a Lepidopteran Pest of Cotton

Author:
Neil I. Morrison; Gregory S. Simmons; Guoliang Fu; Sinead O’Connell; Adam S. Walker; Tarig Dafa’alla; Michelle Walters; John Claus; Guolei Tang; Li Jin; Thea Marubbi; Matthew J. Epton; Claire L. Harris; Robert T. Staten; Ernest Miller; Thomas A. Miller; Luke Alphey; Subba Reddy Palli
Source:
PloS one v.7 no.12 pp. -
ISSN:
1932-6203
Subject:
DNA; Gossypium hirsutum; Pectinophora gossypiella; antidotes; control methods; cotton; death; genetically modified organisms; host plants; insect pests; larvae; mortality; progeny; sterile insect technique; sustainable technology
Abstract:
... The sterile insect technique (SIT) is an environmentally friendly method of pest control in which insects are mass-produced, irradiated and released to mate with wild counterparts. SIT has been used to control major pest insects including the pink bollworm (Pectinophora gossypiella Saunders), a global pest of cotton. Transgenic technology has the potential to overcome disadvantages associated with ...
DOI:
10.1371/journal.pone.0050922
PubMed:
23226548
PubMed Central:
PMC3514271

7. First Report of Blight Disease on Buxus Caused by Cylindrocladium buxicola in France

Author:
Saurat C.; Fourrier C.; Ioos R.
Source:
Plant disease 2012 v.96 no.7 pp. 1069
ISSN:
0191-2917
Subject:
Buxus sempervirens; Calonectria; Cylindrocladium; DNA; agar; blight; branches; chloramphenicol; conidia; conidiophores; death; defoliation; disease occurrence; forestry; fruiting; fruiting bodies; fungi; gardens; genetic databases; hyphae; industry; internal transcribed spacers; lactic acid; leaves; malt; mycelium; polymerase chain reaction; pressing; seedlings; sequence analysis; teleomorphs; France; New Zealand; United Kingdom
Abstract:
... Cylindrocladium buxicola Henricot causes twig blight on Buxus spp., severe defoliation, and eventually death of plants, especially in young seedlings (1). The disease was first observed in the United Kingdom and New Zealand in the 1990s and recently, the fungus was detected in other European countries (1). In November 2006, box blight symptoms were observed in a Buxus sempervirens nursery located ...
DOI:
10.1094/PDIS-03-12-0235-PDN

8. High glucose-induced Ca²⁺ overload and oxidative stress contribute to apoptosis of cardiac cells through mitochondrial dependent and independent pathways

Author:
Sandeep Kumar; Vasundhara Kain; Sandhya L. Sitasawad
Source:
Biochimica et biophysica acta 2012 v.1820 no.7 pp. 907-920
ISSN:
0304-4165
Subject:
DNA; Western blotting; apoptosis; calcium; cardiomyocytes; cardiomyopathy; caspase-12; caspase-9; confocal microscopy; death; flow cytometry; glucose; mitochondria; myoblasts; nitrogen; oxidative stress; plasma membrane; rats; reactive oxygen species
Abstract:
... BACKGROUND: Cardiac cell apoptosis is the initiating factor of cardiac complications especially diabetic cardiomyopathy. Mitochondria are susceptible to the damaging effects of elevated glucose condition. Calcium overload and oxidative insult are the two mutually non-exclusive phenomena suggested to cause cardiac dysfunction. Here, we examined the effect of high-glucose induced calcium overload in ...
DOI:
10.1016/j.bbagen.2012.02.010

9. Identification and evaluation of two diagnostic markers linked to Fusarium wilt resistance (race 4) in banana (Musa spp.)

Author:
Wei Wang; Yulin Hu; Dequan Sun; Christian Staehelin; Dawei Xin; Jianghui Xie
Source:
Molecular biology reports 2012 v.39 no.1 pp. 451-459
ISSN:
0301-4851
Subject:
DNA; Fusarium oxysporum f. sp. cubense; Fusarium wilt; Musa; bananas; cultivars; death; fungi; genotype; marker-assisted selection; micropropagation; molecular biology; plantlets; quality control; random amplified polymorphic DNA technique
Abstract:
... Fusarium wilt caused by the fungus Fusarium oxysporum f. sp. cubense race 4 (FOC4) results in vascular tissue damage and ultimately death of banana (Musa spp.) plants. Somaclonal variants of in vitro micropropagated banana can hamper success in propagation of genotypes resistant to FOC4. Early identification of FOC4 resistance in micropropagated banana plantlets is difficult, however. In this stud ...
DOI:
10.1007/s11033-011-0758-6

10. Maintenance of imaginal disc plasticity and regenerative potential in Drosophila by p53

Author:
Brent S. Wells; Laura A. Johnston
Source:
Developmental biology 2012 v.361 no.2 pp. 263-276
ISSN:
0012-1606
Subject:
DNA; DNA damage; DNA repair; Drosophila; adults; animal physiology; cell death; death; genome; imaginal discs; irradiation; mutants; tissue repair
Abstract:
... Following irradiation (IR), the DNA damage response (DDR) activates p53, which triggers death of cells in which repair cannot be completed. Lost tissue is then replaced and re-patterned through regeneration. We have examined the role of p53 in co-regulation of the DDR and tissue regeneration following IR damage in Drosophila. We find that after IR, p53 is required for imaginal disc cells to repair ...
DOI:
10.1016/j.ydbio.2011.10.012
PubMed:
22036477
PubMed Central:
PMC3296280

11. Molecular detection of Coxiella burnetii and Neospora caninum in equine aborted foetuses and neonates

Author:
Albertine Leon; Eric Richard; Christine Fortier; Claire Laugier; Guillaume Fortier; Stéphane Pronost
Source:
Preventive veterinary medicine 2012 v.104 no.1-2 pp. 179-183
ISSN:
0167-5877
Subject:
Chlamydophila abortus; Coxiella burnetii; DNA; Neospora caninum; bacteria; death; fetal death; horses; industry; molecular biology; mortality; necropsy; neonates; pathogens; polymerase chain reaction; surveys
Abstract:
... Abortion, stillbirth and neonatal death are major causes of equine mortality and cause severe economic loss to the equine industry. The present study was based on a complete necropsy protocol associated with classical microbiological examinations and molecular biology on 407 cases of abortion, stillbirths and neonate death. Based on this retrospective survey, “less common” abortive infectious agen ...
DOI:
10.1016/j.prevetmed.2011.11.001
PubMed:
22130310

12. New species of Gondwanamyces from dying Euphorbia trees in South Africa

Author:
Johannes Alwyn van der Linde; Diana L. Six; Michael J. Wingfield; Jolanda Roux
Source:
Mycologia 2012 v.104 no.2 pp. 574-584
ISSN:
1557-2536
Subject:
Cecropia; Coleoptera; DNA; Euphorbia; Protea; anamorphs; death; fungi; new species; nucleotide sequences; tissues; trees; Central America; South Africa
Abstract:
... Gondwanamyces and its Custingophora anamorphs were first described from Protea infructescences in South Africa. Subsequently these unusual fungi were also found on Cecropia in Central America. During an investigation into the decline and death of native Euphorbia trees in South Africa, several fungal isolates resembling the anamorph state of Gondwanamyces were obtained from diseased tissues. In th ...
DOI:
10.3852/11-166

13. The PPAR-γ agonist troglitazone antagonizes survival pathways induced by STAT-3 in recombinant interferon-β treated pancreatic cancer cells

Author:
Giovanni Vitale; Silvia Zappavigna; Monica Marra; Alessandra Dicitore; Stefania Meschini; Maria Condello; Giuseppe Arancia; Sara Castiglioni; Paola Maroni; Paola Bendinelli; Roberta Piccoletti; Peter M. van Koetsveld; Francesco Cavagnini; Alfredo Budillon; Alberto Abbruzzese; Leo J. Hofland; Michele Caraglia
Source:
Biotechnology Advances 2012 v.30 no.1 pp. 169-184
ISSN:
0734-9750
Subject:
DNA; agonists; apoptosis; autophagy; cell cycle checkpoints; cell growth; death; growth retardation; interferon-beta; interphase; mitogen-activated protein kinase; neoplasm cells; pancreatic neoplasms; phosphorylation; synergism
Abstract:
... We have previously shown that cancer cells can protect themselves from apoptosis induced by type I interferons (IFNs) through a ras→MAPK-mediated pathway. In addition, since IFN-mediated signalling components STATs are controlled by PPAR gamma we studied the pharmacological interaction between recombinant IFN-β and the PPAR-γ agonist troglitazone (TGZ). This combination induced a synergistic effec ...
DOI:
10.1016/j.biotechadv.2011.08.001

14. Phenotypic, molecular phylogenetic, and pathogenetic characterization of Fusarium crassistipitatum sp. nov., a novel soybean sudden death syndrome pathogen from Argentina and Brazil.

Author:
Takayuki Aoki; Maria Mercedes Scandiani; Kerry O'Donnell
Source:
Mycoscience 2012 v.53 no.3 pp. 167-186
ISSN:
1340-3540
Subject:
DNA; Fusarium; Glycine max; Phaseolus vulgaris; Vigna; beans; conidia; conidiophores; data collection; death; foliar diseases; fungal diseases of plants; nucleotide sequences; pathogenicity; phylogeny; plant pathogenic fungi; root rot; signs and symptoms (plants); soybeans; Argentina; Brazil
Abstract:
... A novel soybean sudden death syndrome (SDS) pathogen from Argentina and Brazil is formally described herein as Fusarium crassistipitatum based on detailed phenotypic analyses of macro- and microscopic characters and phylogenetic analyses of multilocus DNA sequence data. Fusarium crassistipitatum can be distinguished from the other soybean SDS and bean (Phaseolus/Vigna) root rot pathogens (BRR) phe ...
Handle:
10113/57558
DOI:
10.1007/s10267-011-0150-3

15. Should we screen for hereditary hemochromatosis in healthy Lebanese: a pilot study

Author:
Rami A. R. Mahfouz; Doja S. Sarieddine; Khalil M. Charafeddine; Rabab N. Abdul Khalik; Najwa K. Cortas; Rose T. Daher
Source:
Molecular biology reports 2012 v.39 no.1 pp. 753-759
ISSN:
0301-4851
Subject:
DNA; blood serum; death; ferritin; gene frequency; genetic disorders; hemochromatosis; heterozygosity; homozygosity; iron; iron absorption; molecular biology; mutation; mutational analysis; phenotype; transferrin; Lebanon
Abstract:
... Hereditary hemochromatosis (HHC) is a genetic disorder of iron metabolism characterized by abnormal accumulation of iron that may lead to organ damage and death. Diagnosis is usually based on various genetic and phenotypic criteria. The study goals were to perform mutation analysis for 18 different mutations associated with HHC in healthy Lebanese, determine their allele frequency, and compare iro ...
DOI:
10.1007/s11033-011-0795-1

16. Vibrio alginolyticus thermolabile hemolysin (TLH) induces apoptosis, membrane vesiculation and necrosis in sea bream erythrocytes

Author:
Sze Ki Wong; Xiao-Hua Zhang; Norman Y.S. Woo
Source:
Aquaculture 2012 v.330-333 no. pp. 29-36
ISSN:
0044-8486
Subject:
DNA; DNA fragmentation; Sparus; Vibrio alginolyticus; apoptosis; bream; caspase-3; death; erythrocytes; flow cytometry; hemoglobin; necrosis; nuclear membrane; plasma membrane; population size; transmission electron microscopy
Abstract:
... Nucleated erythrocytes of silver sea bream (Sparus sarba) were found to enter a unique death mode exhibiting both features of apoptosis and necrosis after exposure to a recombinant thermolabile hemolysin (TLH) of Vibrio alginolyticus. TLH induced significant increases in caspase-3, -8, -9/6 activities followed by apoptotic DNA fragmentation (TUNEL and DNA ladder assays). Apart from these typical a ...
DOI:
10.1016/j.aquaculture.2011.12.012

17. Zinc and Liver Disease

Author:
Mohammad K. Mohammad; Zhanxiang Zhou; Matthew Cave; Ashutosh Barve; Craig J. McClain
Source:
Nutrition in clinical practice v.27 no.1 pp. 8-20
ISSN:
0884-5336
Subject:
DNA; RNA; adverse effects; cell division; cell growth; clinical trials; death; enzymes; excretion; fibrosis; hepatitis C; human diseases; liver function; liver regeneration; metabolism; metallothionein; nausea; oxidative stress; patients; skin lesions; tissue repair; transcription factors; transporters; zinc
Abstract:
... Zinc is an essential trace element required for normal cell growth, development, and differentiation. It is involved in DNA synthesis, RNA transcription, and cell division and activation. It is a critical component in many zinc protein/enzymes, including critical zinc transcription factors. Zinc deficiency/altered metabolism is observed in many types of liver disease, including alcoholic liver dis ...
DOI:
10.1177/0884533611433534
PubMed:
22307488
PubMed Central:
PMC6027651

18. A defect in dystrophin causes a novel porcine stress syndrome

Author:
Dan J. Nonneman; Tami Brown-Brandl; Shuna A. Jones; Ralph T. Wiedmann; Gary A. Rohrer
Source:
BMC genomics 2012 v.13 no.1 pp. 1-9
ISSN:
1471-2164
Subject:
pork; arrhythmia; vocalization; heart; genetic markers; barrows; DNA; males; skeletal muscle; slaughter weight; genotype; biological resistance; skin (animal); isoflurane; genetic polymorphism; breathing; heart rate; cardiomyopathy; muscle fibers; humans; discoloration; herds; arginine; models; electrocardiography; dystrophin; animal transport; progeny; exons; histopathology; malignant hyperthermia; anesthesia; animal stress; disease prevalence; stress response; muscular dystrophy; creatine kinase; tryptophan; death; pedigree
Abstract:
... Background: Losses of slaughter-weight pigs due to transport stress are both welfare and economic concerns to pork producers. Historically, the HAL-1843 mutation in ryanodine receptor 1 was considered responsible for most of the losses; however, DNA testing has effectively eliminated this mutation from commercial herds. We identified two sibling barrows in the USMARC swine herd that died from appa ...
Handle:
10113/57889
DOI:
10.1186/1471-2164-13-233
PubMed:
22691118
PubMed Central:
PMC3463461

19. Aging Process in Chromatin of Animals

Author:
Maciej Wnuk; Monika Bugno-Poniewierska; Anna Lewińska; Bernadetta Oklejewicz; Tomasz Ząbek; Ewa Słota
Source:
Annals of animal science 2012 v.12 no.3 pp. 301-309
ISSN:
1642-3402
Subject:
DNA; DNA damage; animals; apoptosis; cell senescence; chromatin; death; genetic factors; homeostasis; methylation; somatic cells; telomeres
Abstract:
... The aging process is a variable, stochastic and pleiotropic phenomenon which is regulated by different environmental and genetic factors. The age-associated changes, which occur at the molecular and cellular levels and disturb biological homeostasis, may directly or indirectly contribute to aging, causing apoptosis or cellular senescence and consequently leading to the death of the organism. In th ...
DOI:
10.2478/v10220-012-0025-3

20. Arabidopsis ETHE1 Encodes a Sulfur Dioxygenase That Is Essential for Embryo and Endosperm Development

Author:
Meghan M. Holdorf; Heather A. Owen; Sarah Rhee Lieber; Li Yuan; Nicole Adams; Carole Dabney-Smith; Christopher A. Makaroff
Source:
Plant physiology 2012 v.160 no.1 pp. 226-236
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; sulfur; lesions (animal); humans; sulfides; loss-of-function mutation; DNA; Homo sapiens; brain; metabolic diseases; encephalopathy; endosperm; genes; mitochondria; death; homozygosity; embryogenesis; excretion
Abstract:
... Mutations in human (Homo sapiens) ETHYLMALONIC ENCEPHALOPATHY PROTEIN1 (ETHE1) result in the complex metabolic disease ethylmalonic encephalopathy, which is characterized in part by brain lesions, lactic acidemia, excretion of ethylmalonic acid, and ultimately death. ETHE1 -like genes are found in a wide range of organisms; however, the biochemical and physiological role(s) of ETHE1 have not been ...
DOI:
10.1104/pp.112.201855
PubMed:
22786886
PubMed Central:
PMC3440201

21. Cancers predispose neutrophils to release extracellular DNA traps that contribute to cancer-associated thrombosis

Author:
Mélanie Demers; Daniela S. Krause; Daphne Schatzberg; Kimberly Martinod; Jaymie R. Voorhees; Tobias A. Fuchs; David T. Scadden; Denisa D. Wagner
Source:
Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.32 pp. 13076-13081
ISSN:
0027-8424
Subject:
DNA; animal models; breast neoplasms; chromatin; death; granulocyte colony-stimulating factor; leukemia; lung neoplasms; mice; neutrophils; patients; prognosis; thrombocytopenia; thrombosis
Abstract:
... Cancer-associated thrombosis often lacks a clear etiology. However, it is linked to a poor prognosis and represents the second-leading cause of death in cancer patients. Recent studies have shown that chromatin released into blood, through the generation of neutrophil extracellular traps (NETs), is procoagulant and prothrombotic. Using a murine model of chronic myelogenous leukemia, we show that m ...
DOI:
10.1073/pnas.1200419109
PubMed:
22826226
PubMed Central:
PMC3420209

22. Chloroplastic NADPH oxidase-like activity-mediated perpetual hydrogen peroxide generation in the chloroplast induces apoptotic-like death of Brassica napus leaf protoplasts

Author:
Rajesh Kumar Tewari; Daisuke Watanabe; Masami Watanabe
Source:
Planta 2012 v.235 no.1 pp. 99-110
ISSN:
0032-0935
Subject:
Brassica napus; DNA; NADP (coenzyme); antioxidant activity; ascorbate peroxidase; cell death; chloroplasts; death; hydrogen peroxide; leaves; lipid peroxidation; membrane potential; peroxidase; protoplasts; superoxide dismutase
Abstract:
... Despite extensive research over the past years, regeneration from protoplasts has been observed in only a limited number of plant species. Protoplasts undergo complex metabolic modification during their isolation. The isolation of protoplasts induces reactive oxygen species (ROS) generation in Brassica napus leaf protoplasts. The present study was conducted to provide new insight into the mechanis ...
DOI:
10.1007/s00425-011-1495-8
PubMed:
21853253

23. ETosis: A Microbicidal Mechanism beyond Cell Death

Author:
Guimarães-Costa Anderson B.; Nascimento Michelle T. C.; Wardini Amanda B.; Pinto-da-Silva Lucia H.; Saraiva Elvira M.; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; Conselho Nacional de Desenvolvimento Científico e Tecnológico; Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
Source:
Journal of parasitology research 2012 v.2012 pp. -
ISSN:
2090-0031
Subject:
DNA; Protozoa; cell death; death; eosinophils; histones; macrophages; mast cells; neutrophils; parasites
Abstract:
... Netosis is a recently described type of neutrophil death occurring with the release to the extracellular milieu of a lattice composed of DNA associated with histones and granular and cytoplasmic proteins. These webs, initially named neutrophil extracellular traps (NETs), ensnare and kill microorganisms. Similarly, other cell types, such as eosinophils, mast cells, and macrophages, can also dye by ...
DOI:
10.1155/2012/929743
PubMed:
22536481
PubMed Central:
PMC3321301

24. First Report of Pilidiella granati Causing Dieback and Fruit Rot of Pomegranate (Punica granatum) in Iran

Author:
Mirabolfathy M.; Groenewald J. Z.; Crous P. W.
Source:
Plant disease 2012 v.96 no.3 pp. 461
ISSN:
0191-2917
Subject:
DNA; Punica granatum; agar; bark; blight; branches; conidia; death; dieback; fruits; fungi; genetic databases; greenhouse production; internal transcribed spacers; malt extract; mycelium; operon; pathogenicity; pathogens; plant rots; plastic bags; pomegranates; pycnidia; roots; spring; temperature; trees; Iran; United States
Abstract:
... Iran is the largest producer of pomegranate (Punica granatum) in the world, with more than 60,000 ha currently in production. In the spring of 2011, a decline and dieback of young pomegranate trees (7 to 10 years old) were observed in the Kheir area of Fars Province. Dieback and twig blight developed toward the lower part of the stem, resulting in death of aerial tree parts and growing suckers fro ...
DOI:
10.1094/PDIS-10-11-0844

25. Heterochromatin and gene positioning: inside, outside, any side?

Author:
K. Laurence Jost; Bianca Bertulat; M. Cristina Cardoso
Source:
Chromosoma 2012 v.121 no.6 pp. 555-563
ISSN:
0009-5915
Subject:
DNA; death; epigenetics; eukaryotic cells; gene expression; genes; heterochromatin; transcription (genetics)
Abstract:
... All cellular processes depend on the expression and repression of the right sets of genes at the right time. As each cell contains the same DNA, transcriptional and epigenetic factors have to maintain tight control over gene expression. Even a small divergence from the correct transcriptional program can lead to severe defects and even death. Having deciphered the complete linear genetic informati ...
DOI:
10.1007/s00412-012-0389-2
PubMed:
23090282
PubMed Central:
PMC3501169

26. Ibex-Associated Malignant Catarrhal Fever–Like Disease in a Group of Bongo Antelope (Tragelaphus eurycerus)

Author:
D. Gasper; B. Barr; H. Li; N. Taus; R. Peterson; G. Benjamin; T. Hunt; P. A. Pesavento
Source:
Veterinary pathology v.49 no.3 pp. 492-497
ISSN:
1544-2217
Subject:
Capra ibex; DNA; DNA-directed DNA polymerase; Tragelaphus; antelopes; case studies; death; distress; edema; liver; malignant catarrhal fever; myocarditis; polymerase chain reaction; vasculitis
Abstract:
... A private zoological facility experienced an outbreak of malignant catarrhal fever (MCF) in 3 bongo antelope (Tragelaphus eurycerus). All cases were periparturient bongos that presented acutely anorectic beginning ~6 weeks after being housed with a Nubian ibex. Disease quickly progressed to respiratory distress and death within 24–72 hours of onset of clinical signs. Consistent gross findings in a ...
DOI:
10.1177/0300985811429306
PubMed:
22194357

27. Improvement of the post-thaw qualities of Okinawan native Agu pig sperm frozen in an extender supplemented with antiapoptotic PTD-FNK protein

Author:
Kazuki Shimokawa; Ryuko Oshiro; Kenichi Yamanaka; Koji Ashizawa; Shigeo Ohta; Hideki Tatemoto
Source:
Theriogenology 2012 v.78 no.7 pp. 1446-1455
ISSN:
0093-691X
Subject:
DNA; adenosine triphosphate; boars; breeding; caspase-8; caspase-9; cryopreservation; death; freeze-thaw cycles; freezing; oocytes; sperm motility; spermatozoa
Abstract:
... The technical establishment of boar sperm cryopreservation is indispensable for effective breeding of the scarce Okinawan native Agu pig. The objective was to determine whether an artificial anticell death protein (PTD-FNK protein) was capable of improving the quality of cryopreserved Agu sperm. Ejaculated Agu sperm frozen in an extender supplemented with 0, 100, 200, 300, or 400 nm PTD-FNK protei ...
DOI:
10.1016/j.theriogenology.2012.06.005
PubMed:
22925637

28. Influence of functional genetic polymorphism (−590C/T) in non-small cell lung cancer (NSCLC) development: The paradoxal role of IL-4

Author:
Mónica Gomes; Ana Coelho; António Araújo; Ana Luísa Teixeira; Raquel Catarino; Rui Medeiros
Source:
Gene 2012 v.504 no.1 pp. 111-115
ISSN:
0378-1119
Subject:
DNA; death; genes; genotype; inflammation; interleukin-4; lung neoplasms; monocytes; pathogenesis; patients; polymerase chain reaction; promoter regions; restriction fragment length polymorphism; single nucleotide polymorphism
Abstract:
... Lung cancer is the leading cause of death by cancer in the world, originating about 17.5% of total deaths from cancer (1.18 million). Inflammation plays an important role in the pathogenesis of lung cancer. IL-4 is an anti-inflammatory cytokine, which reduces the production of proinflammatory cytokines by monocytes and with direct antiproliferative effects in some tumors. The polymorphism −590C/T ...
DOI:
10.1016/j.gene.2012.05.008

29. Inhibition of NAMPT pathway by FK866 activates the function of p53 in HEK293T cells

Author:
Basant Kumar Thakur; Tino Dittrich; Prakash Chandra; Annette Becker; Yannick Lippka; Divakarvel Selvakumar; Jan-Henning Klusmann; Dirk Reinhardt; Karl Welte
Source:
Biochemical and biophysical research communications 2012 v.424 no.3 pp. 371-377
ISSN:
0006-291X
Subject:
DNA; acetylation; antigens; apoptosis; carcinogens; cell cycle; cell proliferation; death; humans; models; nicotinamide; pathogenesis; viruses
Abstract:
... Inactivation of p53 protein by endogenous and exogenous carcinogens is involved in the pathogenesis of different human malignancies. In cancer associated with SV-40 DNA tumor virus, p53 is considered to be non-functional mainly due to its interaction with the large T-antigen. Using the 293T cell line (HEK293 cells transformed with large T antigen) as a model, we provide evidence that p53 is one of ...
DOI:
10.1016/j.bbrc.2012.06.075

30. Lipid-like components released from degenerating dopaminergic neurons trigger the dynamic migration of microglia

Author:
Hyemin Kim; June-Hee Park; Kyungjin Kim
Source:
Biochemical and biophysical research communications 2012 v.426 no.1 pp. 18-25
ISSN:
0006-291X
Subject:
DNA; Parkinson disease; RNA; brain; death; models; neuroglia; neurons; pellets; proteins; ultracentrifugation
Abstract:
... In the brain, communication between neural and non-neural cells is crucial for the proper functioning of the central nervous system. Microglia play an important role in the clearance of neural cellular corpses and debris, especially under pathological conditions. It remains, however, unclear how microglia sense the degenerating neurons at a distance in order to migrate to them. In the present stud ...
DOI:
10.1016/j.bbrc.2012.07.167

31. Macrocyclic lactone toxicity due to abamectin in farm dogs without the ABCB1 gene mutation

Author:
K Parton; EM Wiffen; ND Haglund; NJ Cave
Source:
New Zealand veterinary journal 2012 v.60 no.3 pp. 194-197
ISSN:
1176-0710
Subject:
DNA; abamectin; ataxia (disorder); beef; blindness; calves; death; dogs; farms; females; gait; genes; hematology; ingestion; muscles; mutation; poisoning; product labeling; risk; sheep; toxicity
Abstract:
... CASE HISTORY: A 5-year-old entire female Huntaway from a sheep and beef farm was one of four dogs that developed clinical signs including hypersalivation, depression, blindness and ataxia after the death of another dog. A 4-year-old female Huntaway farm dog from a second farm was observed to be sitting down more often than usual on the day after being fed part of a calf carcass that had been treat ...
DOI:
10.1080/00480169.2011.642770

32. Obtaining an Isolate of Ancylostoma braziliense From Cats Without the Need for Necropsy

Author:
Liotta Janice L.; Lee Alice C. Y.; Koompapong Khuanchai N.; Yaros Joseph P.; Prullage Joseph; Ulrich Michael A.; Bowman Dwight D.
Source:
Journal of parasitology 2012 v.98 no.5 pp. 1037-1038
ISSN:
0022-3395
Subject:
Ancylostoma tubaeforme; DNA; cats; death; eggs; feces; harvesting; larvae; necropsy; puppies; restriction fragment length polymorphism; sequence analysis; specific pathogen-free animals; sugars; Florida
Abstract:
... Because the eggs of Ancylostoma species of dogs and cats are difficult to readily distinguish morphologically, isolation of a certain species often requires the humane death of the source animal or holding an animal after treatment to obtain worms for specific identification or to harvest ex utero eggs. The objective of this study was to obtain an isolate of Ancylostoma braziliense from 1-time, fi ...
DOI:
10.1645/GE-2981.1

33. Obtaining an Isolate of Ancylostoma braziliense From Dogs Without the Need for Necropsy

Author:
Liotta Janice L.; Lee Alice C. Y.; Aksel Sarp; Alkhalife Ibrahim; Cruz-Reyes Alejandro; Youn HeeJeong; Bienhoff Stephen E.; Bowman Dwight D.
Source:
Journal of parasitology 2012 v.98 no.5 pp. 1034-1036
ISSN:
0022-3395
Subject:
Ancylostoma caninum; Beagle; DNA; adults; death; dogs; eggs; endoscopy; feces; females; kittens; larvae; males; necropsy; restriction fragment length polymorphism; sequence analysis; sugars; Florida
Abstract:
... Isolation of a specific Ancylostoma species typically requires death of the source animal, or holding an animal long enough to collect feces after treatment, for worm recovery and identification. The reason for collecting worms is that the eggs are not easy to distinguish morphologically. In keeping with the 3 Rs of laboratory animal research (reduction, refinement, replacement), the objective of ...
DOI:
10.1645/GE-2938.1

34. Piceatannol Suppresses Breast Cancer Cell Invasion through the Inhibition of MMP-9: Involvement of PI3K/AKT and NF-κB Pathways

Author:
Ko Hyun Suk; Lee Hyo-Jeong; Kim Sung-Hoon; Lee Eun-Ok
Source:
Journal of agricultural and food chemistry 2012 v.60 no.16 pp. 4083-4089
ISSN:
1520-5118
Subject:
phosphatidylinositol 3-kinase; transcription factor NF-kappa B; viability; messenger RNA; gelatinase A; DNA; resveratrol; transcription (genetics); metastasis; cytoplasm; adhesion; mammals; patients; death; phosphorylation; breast neoplasms
Abstract:
... Cancer invasion and metastasis are the main causes of treatment failure and death in cancer patients. Piceatannol (3,3′,4,5′-tetrahydroxy-trans-stilbene) is a natural analogue of resveratrol. This study investigated the anti-invasive mechanisms of piceatannol in MDA-MB-231 cells. Piceatannol significantly reduced serum-induced cell invasion and migration as well as adhesion without affecting the v ...
DOI:
10.1021/jf205171g
PubMed:
22480333

35. Screening of genomic regions affecting lawc/Trf2 gene expression during Drosophila melanogaster development

Author:
O. B. Simonova; E. A. Modestova; J. E. Vorontsova; R. O. Cherezov
Source:
Russian journal of developmental biology 2012 v.43 no.5 pp. 301-317
ISSN:
1062-3604
Subject:
DNA; Drosophila melanogaster; TATA box; binding proteins; chromosome mapping; chromosome morphology; death; gene expression; genes; germ cells; humans; loci; male reproductive system; males; mutants; mutation; nucleosomes; phenotype; progeny; screening; transcription (genetics); transcription factors
Abstract:
... leg-arista-wing complex (lawc) mutations affect the expression of Drosophila proteins homologous to a human basic transcription factor, TBP (TATA-box binding protein) Related Factor 2 (TRF2), specifically involved in development.The paper for the first time reports the application of genetic screens for various genomic regions to identify genetic interactions between the lawc/Trf2 gene and other g ...
DOI:
10.1134/S1062360412050086

36. Selective death of cancer cells by preferential induction of reactive oxygen species in response to (-)-epigallocatechin-3-gallate

Author:
Na Young Min; Jin-Hong Kim; Jee-Hye Choi; Wen Liang; Young Jong Ko; Sangmyung Rhee; Hyoweon Bang; Seung Wook Ham; Ae Ja Park; Kwang-Ho Lee
Source:
Biochemical and biophysical research communications 2012 v.421 no.1 pp. 91-97
ISSN:
0006-291X
Subject:
DNA; apoptosis; carcinogenesis; cytotoxicity; death; drug therapy; epigenetics; gene expression regulation; genes; humans; hydrogen peroxide; neoplasm cells; promoter regions; telomerase
Abstract:
... (-)-Epigallocatechin-3-gallate (EGCG) induces apoptosis in cancer cells without adversely affecting normal cells. Understanding the cancer-specific cytotoxic activity of EGCG is very important in defining the mechanism of tumorigenesis and identifying superb chemotherapeutic agents against cancer. We comparatively assayed human telomerase reverse transcriptase (hTERT)-mediated apoptosis by EGCG-in ...
DOI:
10.1016/j.bbrc.2012.03.120

37. Symptomatic and Asymptomatic Host Range of Fusarium virguliforme, the Causal Agent of Soybean Sudden Death Syndrome

Author:
Kolander T. M.; Bienapfl J. C.; Kurle J. E.; Malvick D. K.
Source:
Plant disease 2012 v.96 no.8 pp. 1148-1153
ISSN:
0191-2917
Subject:
Astragalus; Chenopodium album; DNA; Fusarium virguliforme; Lolium; Trifolium pratense; alfalfa; biomass; canola; corn; crop rotation; crops; death; greenhouses; host range; hosts; inoculum; navy beans; necrosis; pathogens; peas; polymerase chain reaction; roots; soil; soybean sudden death syndrome; soybeans; sugar beet; weeds; wheat; United States
Abstract:
... Sudden death syndrome, caused by Fusarium virguliforme, is an important disease of soybean in the United States. Fifteen species of crops, weeds, or prairie plants were evaluated for their potential as hosts of F. virguliforme. Root and foliar symptoms and plant biomass were assessed following greenhouse inoculation studies. Root colonization of F. virguliforme was determined with isolations and w ...
DOI:
10.1094/PDIS-08-11-0685-RE

38. Unraveling a mechanism of honey antibacterial action: Polyphenol/H₂O₂-induced oxidative effect on bacterial cell growth and on DNA degradation

Author:
Katrina Brudzynski; Kamal Abubaker; Danielle Miotto
Source:
Food chemistry 2012 v.133 no.2 pp. 329-336
ISSN:
0308-8146
Subject:
DNA; DNA damage; chemistry; death; free radical scavengers; honey; hydrogen peroxide; microbial growth; oxidative stress; polyphenols
Abstract:
... Several compounds with antibacterial activities were identified in honey however, a mechanism by which they lead to bacterial growth inhibition and bacterial death remains still unknown. We recently found that honeys possess DNA degrading activity mediated by honey hydrogen peroxide and an unknown honey component(s). Here we provide evidence that active honeys (MIC₉₀ of 6.25–12.5% v/v) possessed s ...
DOI:
10.1016/j.foodchem.2012.01.035

39. Vaccinia viruses isolated from cutaneous disease in horses are highly virulent for rabbits

Author:
Juliana Felipetto Cargnelutti; Candice Schmidt; Eduardo Kenji Masuda; Lisiane Danusa Braum; Rudi Weiblen; Eduardo Furtado Flores
Source:
Microbial pathogenesis 2012 v.52 no.3 pp. 192-199
ISSN:
0882-4010
Subject:
DNA; animal models; bronchopneumonia; death; distress; euthanasia; feces; horses; necropsy; neutralizing antibodies; nose; rabbits; viral load; viral shedding; viremia; virulence; viruses; weanlings; Brazil
Abstract:
... Two genotypically distinct Vaccinia viruses (VACV), named P1V and P2V, were isolated from an outbreak of cutaneous disease in horses in Southern Brazil. We herein investigated the susceptibility of rabbits, a proposed animal model, to P1V and P2V infection. Groups of weanling rabbits were inoculated intranasally (IN) with P1V or P2V at low (10².⁵ TCID₅₀), medium (10⁴.⁵TCID₅₀), or high titer (10⁶.⁵ ...
DOI:
10.1016/j.micpath.2011.12.005

40. Verticillium Wilt of Spineless Safflower Caused by Verticillium dahliae in California

Author:
Koike S. T.; Baameur A.; Maruthachalam K.; Subbarao K. V.
Source:
Plant disease 2012 v.96 no.9 pp. 1383
ISSN:
0191-2917
Subject:
Carthamus tinctorius; DNA; Sphagnum; Verticillium dahliae; Verticillium wilt; buds; conidia; conidiophores; crop rotation; crops; death; dieback; discoloration; flowers; fungi; greenhouses; inoculum; leaves; mycelium; oilseed crops; pathogenicity; plant pathology; polymerase chain reaction; sclerotia; soil; soil drenching; strawberries; wilting; California
Abstract:
... Spineless selections of Carthamus tinctorius (safflower) are grown as commercial field grown cutflower crops in coastal California. In 2010, field plantings of spineless safflower in Santa Clara County developed symptoms of a wilt disease. Affected plants were stunted and slow to develop. As plants developed flower buds, lower leaves turned yellow and wilted. As disease developed, lower leaves tur ...
DOI:
10.1094/PDIS-02-12-0222-PDN

41. “Delayed death” phenomenon: A synergistic action of cyclophosphamide and exogenous DNA

Author:
Evgenia V. Dolgova; Anastasia S. Proskurina; Valeriy P. Nikolin; Nelly A. Popova; Ekaterina A. Alyamkina; Konstantin E. Orishchenko; Vladimir A. Rogachev; Yaroslav R. Efremov; Tatiana D. Dubatolova; Anastasia V. Prokopenko; Elena R. Chernykh; Alexandr A. Ostanin; Oleg S. Taranov; Vladimir V. Omigov; Stanislav N. Zagrebelniy; Sergey S. Bogachev; Mikhail A. Shurdov
Source:
Gene 2012 v.495 no.2 pp. 134-145
ISSN:
0378-1119
Subject:
DNA; DNA damage; DNA repair; apoptosis; bone marrow cells; cyclophosphamide; death; histopathology; mice; morbidity; mortality; pretreatment; stem cells
Abstract:
... Morbidity and mortality in mice were observed upon administration of exogenous DNA following their pre-treatment with a cytostatic agent cyclophosphamide. Upon intraperitoneal injections, the fragments of exogenous DNA reached bone marrow cells. These cells were also found to internalize up to 1800kb of exogenous DNA ex vivo. The 18–24h time frame represents a final stage in the repair of DNA doub ...
DOI:
10.1016/j.gene.2011.12.032
PubMed:
22227496