Publication Year: 2012 / Text availability: Full Text

1. "Evolution Canyon," a potential microscale monitor of global warming across life

Author:: Eviatar Nevo
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.8 pp. 2960-2965
ISSN:: 0027-8424
Subject:: Drosophila; bacteria; biodiversity; drought; extinction; fungi; global warming; heat; mammals; microclimate; models; photosynthesis; prediction; shrublands; solar radiation; sympatric speciation; viruses; Israel
Abstract:: ... Climatic change and stress is a major driving force of evolution. The effects of climate change on living organisms have been shown primarily on regional and global scales. Here I propose the "Evolution Canyon" (EC) microscale model as a potential life monitor of global warming in Israel and the rest of the world. The EC model reveals evolution in action at a microscale involving biodiversity dive ...
DOI:: 10.1073/pnas.1120633109
PubMed:: 22308456
PubMed Central:: PMC3286920
: https://doi.org/10.1073/pnas.1120633109

2. "tet(U)" Is Not a Tetracycline Resistance Determinant

Author:: Jamie A. Caryl; Georgina Cox; Stefan Trimble; Alex J. O'Neill
Source:: Antimicrobial agents and chemotherapy 2012 v.56 no.6 pp. 3378-3379
ISSN:: 0066-4804
Subject:: antibiotic resistance; bacterial proteins; bioinformatics; genes; plasmids; resistance mechanisms; tetracycline
Abstract:: ... The enterococcal plasmid pKQ10 has been reported to carry a poorly characterized tetracycline resistance determinant designated tet(U). However, in a series of studies intended to further characterize this determinant, we have been unable to substantiate the claim that tet(U) confers resistance to tetracyclines. In line with these results, bioinformatic analysis provides compelling evidence that " ...
DOI:: 10.1128/AAC.05957-11
PubMed:: 22491689
PubMed Central:: PMC3370814
: https://doi.org/10.1128/AAC.05957-11

3. (n-3) Fatty Acids and Cardiovascular Health: Are Effects of EPA and DHA Shared or Complementary?

Author:: Dariush Mozaffarian; Jason H. Y. Wu
Source:: Journal of nutrition 2012 v.142 no.3 pp. 614S-625S
ISSN:: 0022-3166
Subject:: docosapentaenoic acid; high density lipoprotein; fish consumption; humans; compliance; inflammation; laboratory animals; endogenous sources; particle size; platelet aggregation; low density lipoprotein; risk factors; animal models; arrhythmia; fish oils; clinical trials; oxidative stress; research support
Abstract:: ... Considerable research supports cardiovascular benefits of consuming omega-3 PUFA, also known as (n-3) PUFA, from fish or fish oil. Whether individual long-chain (n-3) PUFA have shared or complementary effects is not well established. We reviewed evidence for dietary and endogenous sources and cardiovascular effects on biologic pathways, physiologic risk factors, and clinical endpoints of EPA [20:5 ...
DOI:: 10.3945/jn.111.149633
PubMed:: 22279134
PubMed Central:: PMC3278271
: https://doi.org/10.3945/jn.111.149633

4. (φ,ψ)₂ Motifs: A Purely Conformation-Based Fine-Grained Enumeration of Protein Parts at the Two-Residue Level

Author:: Scott A. Hollingsworth; Matthew C. Lewis; Donald S. Berkholz; Weng-Keen Wong; P. Andrew Karplus
Source:: Journal of molecular biology 2012 v.416 no.1 pp. 78-93
ISSN:: 0022-2836
Subject:: algorithms; models; polypeptides; population density; protein structure; proteins
Abstract:: ... A deep understanding of protein structure benefits from the use of a variety of classification strategies that enhance our ability to effectively describe local patterns of conformation. Here, we use a clustering algorithm to analyze 76,533 all-trans segments from protein structures solved at 1.2 Å resolution or better to create a purely φ,ψ-based comprehensive empirical categorization of common c ...
DOI:: 10.1016/j.jmb.2011.12.022
PubMed:: 22198294
PubMed Central:: PMC3268948
: https://doi.org/10.1016/j.jmb.2011.12.022

5. (−)-Epicatechin prevents TNFα-induced activation of signaling cascades involved in inflammation and insulin sensitivity in 3T3-L1 adipocytes

Author:: Marcela A. Vazquez-Prieto; Ahmed Bettaieb; Fawaz G. Haj; César G. Fraga; Patricia I. Oteiza
Source:: Archives of biochemistry and biophysics 2012 v.527 no.2 pp. 113-118
ISSN:: 0003-9861
Subject:: adipocytes; adipose tissue; chronic diseases; extracts; genes; inflammation; insulin; insulin resistance; interleukin-6; metabolic syndrome; mitogen-activated protein kinase; noninsulin-dependent diabetes mellitus; nuclear genome; obesity; phosphorylation; public health; resistin; risk; transcription factor NF-kappa B; transcriptional activation; tumor necrosis factor-alpha
Abstract:: ... Obesity is major public health concern worldwide and obese individuals exhibit a higher risk of chronic diseases such as type 2 diabetes. Inflammation plays a significant role in metabolic regulation and mounting evidence highlight the contribution of adipose tissue to systemic inflammatory state. Food extracts with a high content of (−)-epicatechin have been found to exert systemic anti-inflammat ...
DOI:: 10.1016/j.abb.2012.02.019
PubMed:: 22425757
PubMed Central:: PMC3992864
: https://doi.org/10.1016/j.abb.2012.02.019

6. L: -Theanine extends lifespan of adult Caenorhabditis elegans

Author:: Kim Zarse; Saskia Jabin; Michael Ristow
Source:: European journal of nutrition 2012 v.51 no.6 pp. 765-768
ISSN:: 1436-6207
Subject:: Caenorhabditis elegans; Camellia sinensis; Escherichia coli; adults; agar; bacteria; blood; green tea; humans; longevity; medicine; paraquat
Abstract:: ... PURPOSE: Compounds that delay aging in model organisms may be of significant interest to anti-aging medicine, since these substances potentially provide pharmaceutical approaches to promote healthy lifespan in humans. We here aimed to test whether pharmaceutical concentrations of L: -theanine, a putative anti-cancer, anti-obesity, blood pressure-lowering, and neuroprotective compound contained in ...
DOI:: 10.1007/s00394-012-0341-5
PubMed:: 22422488
PubMed Central:: PMC3419347
: https://doi.org/10.1007/s00394-012-0341-5

7. 1,2,3-Triazole–Heme Interactions in Cytochrome P450: Functionally Competent Triazole–Water–Heme Complexes

Author:: Conner Kip P.; Vennam Preethi; Woods Caleb M.; Krzyaniak Matthew D.; Bowman Michael K.; Atkins William M.
Source:: Biochemistry 2012 v.51 no.32 pp. 6441-6457
ISSN:: 1520-4995
Subject:: cytochrome P-450; electron paramagnetic resonance spectroscopy; enthalpy; entropy; heme; heme iron; hydrogen bonding; ligands; metabolism; models; risk
Abstract:: ... In comparison to imidazole (IMZ) and 1,2,4-triazole (1,2,4-TRZ), the isosteric 1,2,3-triazole (1,2,3-TRZ) is unrepresented among cytochrome P450 (CYP) inhibitors. This is surprising because 1,2,3-TRZs are easily obtained via “click” chemistry. To understand this underrepresentation of 1,2,3-TRZs among CYP inhibitors, thermodynamic and density functional theory computational studies were performed ...
DOI:: 10.1021/bi300744z
PubMed:: 22809252
PubMed Central:: PMC3448000
: https://doi.org/10.1021/bi300744z

8. The 1,2-Benzenedithiole-Based Cyclocondensation Assay: A Valuable Tool for the Measurement of Chemopreventive Isothiocyanates

Author:: Yuesheng Zhang
Source:: Critical reviews in food science and nutrition 2012 v.52 no.6 pp. 525-532
ISSN:: 1549-7852
Subject:: chemoprevention; humans; isothiocyanates; mechanism of action; metabolites; vegetables
Abstract:: ... Many naturally occurring isothiocyanates (ITCs) show highly promising chemopreventive activities. Humans are commonly exposed to these compounds through the consumption of cruciferous vegetables which are the main source of dietary ITCs. Dietary ITCs may play an important role in cancer prevention and in the well-recognized cancer preventive activities of cruciferous vegetables. A generic analytic ...
DOI:: 10.1080/10408398.2010.503288
PubMed:: 22452732
PubMed Central:: PMC3315191
: https://doi.org/10.1080/10408398.2010.503288

9. 1,2-dielaidoylphosphocholine/1,2-dimyristoylphosphoglycerol supported phospholipid bilayer formation in calcium and calcium-free buffer

Author:: Evans, Kervin O.
Source:: Thin solid films 2012 v.520 no.7 pp. 3026-3030
ISSN:: 0040-6090
Subject:: atomic force microscopy; buffers; calcium; ions; lipid bilayers; lipid composition; monitoring; phosphatidylcholines; phosphatidylglycerols
Abstract:: ... Phospholipid membranes are useful in the field of biocatalysis because a supported phospholipid membrane can create a biomimetic platform where biocatalytic processes can readily occur. In this work, supported bilayer formation from sonicated phospholipid vesicles containing 1,2-dielaidoyl-sn-glycero-3-phosphocholine and 1,2-dimyristoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] was studied using a q ...
Handle:: 10113/55125
DOI:: 10.1016/j.tsf.2011.12.002
: https://doi.org/10.1016/j.tsf.2011.12.002

10. 1,4-Diazaspiro[2.2]pentanes as a Flexible Platform for the Synthesis of Diamine-Bearing Stereotriads

Author:: Rigoli Jared W.; Boralsky Luke A.; Hershberger John C.; Marston Dagmara; Meis Alan R.; Guzei Ilia A.; Schomaker Jennifer M.
Source:: Journal of organic chemistry 2012 v.77 no.5 pp. 2446-2455
ISSN:: 1520-6904
Subject:: Lewis bases; alcohols; allene; aziridines; bioactive compounds; chemical structure; optical isomerism; organic chemistry; oxidation; regioselectivity
Abstract:: ... Nitrogen-containing stereotriads occur in a number of biologically active compounds, but general and flexible methods to access these compounds are limited mainly to the manipulation of chiral olefins. An alternative approach is to employ a highly chemo-, regio-, and stereocontrolled allene oxidation that can install a new carbon–heteroatom bond at each of the three original allene carbons. In thi ...
DOI:: 10.1021/jo3000282
PubMed:: 22304460
PubMed Central:: PMC4500119
: https://doi.org/10.1021/jo3000282

11. 1,500 year quantitative reconstruction of winter precipitation in the Pacific Northwest

Author:: Byron A. Steinman; Mark B. Abbott; Michael E. Mann; Nathan D. Stansell; Bruce P. Finney
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.29 pp. 11619-11623
ISSN:: 0027-8424
Subject:: El Nino; climate; drought; growth rings; isotopes; lakes; oxygen; paleoclimatology; seasonal variation; sediments; winter
Abstract:: ... Multiple paleoclimate proxies are required for robust assessment of past hydroclimatic conditions. Currently, estimates of drought variability over the past several thousand years are based largely on tree-ring records. We produced a 1,500-y record of winter precipitation in the Pacific Northwest using a physical model-based analysis of lake sediment oxygen isotope data. Our results indicate that ...
DOI:: 10.1073/pnas.1201083109
PubMed:: 22753510
PubMed Central:: PMC3406856
: https://doi.org/10.1073/pnas.1201083109

12. 1-Methylthio-d-xylulose 5-Phosphate Methylsulfurylase: A Novel Route to 1-Deoxy-d-xylulose 5-Phosphate in Rhodospirillum rubrum

Author:: Warlick Benjamin P.; Evans Bradley S.; Erb Tobias J.; Ramagopal Udipi A.; Sriram Jaya; Imker Heidi J.; Sauder J. Michael; Bonanno Jeffrey B.; Burley Stephen K.; Tabita F. Robert; Almo Steven C.; Sweedler Jonathan S.; Gerlt John A.
Source:: Biochemistry 2012 v.51 no.42 pp. 8324-8326
ISSN:: 1520-4995
Subject:: Rhodospirillum rubrum; S-adenosylmethionine; active sites; adenine; biosynthesis; dimethyl disulfide; isomerization; isoprenoids; polyamines
Abstract:: ... Rhodospirillum rubrum produces 5-methylthioadenosine (MTA) from S-adenosylmethionine in polyamine biosynthesis; however, R. rubrum lacks the classical methionine salvage pathway. Instead, MTA is converted to 5-methylthio-d-ribose 1-phosphate (MTR 1-P) and adenine; MTR 1-P is isomerized to 1-methylthio-d-xylulose 5-phosphate (MTXu 5-P) and reductively dethiomethylated to 1-deoxy-d-xylulose 5-phosph ...
DOI:: 10.1021/bi301215g
PubMed:: 23035785
PubMed Central:: PMC3490199
: https://doi.org/10.1021/bi301215g

13. The 10-Year Cost-Effectiveness of Lifestyle Intervention or Metformin for Diabetes Prevention: An intent-to-treat analysis of the DPP/DPPOS

Author:
Source:: Diabetes care 2012 v.35 no.4 pp. 723-730
ISSN:: 0149-5992
Subject:: adults; cost effectiveness; lifestyle; metformin; noninsulin-dependent diabetes mellitus; quality of life; quality-adjusted life year
Abstract:: ... OBJECTIVE: The Diabetes Prevention Program (DPP) and its Outcomes Study (DPPOS) demonstrated that either intensive lifestyle intervention or metformin could prevent type 2 diabetes in high-risk adults for at least 10 years after randomization. We report the 10-year within-trial cost-effectiveness of the interventions. RESEARCH DESIGN AND METHODS: Data on resource utilization, cost, and quality of ...
DOI:: 10.2337/dc11-1468
PubMed:: 22442395
PubMed Central:: PMC3308273
: https://doi.org/10.2337/dc11-1468

14. 100% Orange juice consumption is associated with better diet quality, improved nutrient adequacy, decreased risk for obesity, and improved biomarkers of health in adults: National Health and Nutrition Examination Survey, 2003-2006

Author:: Carol E O’Neil; Theresa A Nicklas; Gail C Rampersaud; Victor L Fulgoni III
Source:: Nutrition journal 2012 v.11 no.1 pp. 565
ISSN:: 1475-2891
Subject:: Adequate Intakes; food groups; odds ratio; folic acid; nutrients; adults; Estimated Average Requirement; biomarkers; orange juice; metabolic syndrome; risk factors; potassium; childhood obesity; USDA; body mass index; vitamin A; ascorbic acid; fruits; nutritional adequacy; regression analysis; magnesium; cholesterol; healthy diet; fruit consumption; National Health and Nutrition Examination Survey; whole grain foods
Abstract:: ... BACKGROUND: Consumption of 100% orange juice (OJ) has been positively associated with nutrient adequacy and diet quality, with no increased risk of overweight/obesity in children; however, no one has examined these factors in adults. The purpose of this study was to examine the association of 100% OJ consumption with nutrient adequacy, diet quality, and risk factors for metabolic syndrome (MetS) i ...
DOI:: 10.1186/1475-2891-11-107
PubMed:: 23234248
PubMed Central:: PMC3545988
: https://doi.org/10.1186/1475-2891-11-107

15. IL- 10–induced microRNA-187 negatively regulates TNF-α, IL-6, and IL-12p40 production in TLR4-stimulated monocytes

Author:: Marzia Rossato; Graziella Curtale; Nicola Tamassia; Monica Castellucci; Laura Mori; Sara Gasperini; Barbara Mariotti; Mariacristina De Luca; Massimiliano Mirolo; Marco A. Cassatella; Massimo Locati; Flavia Bazzoni
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.45 pp. E3101
ISSN:: 0027-8424
Subject:: Toll-like receptor 4; humans; interleukin-10; interleukin-12; interleukin-6; messenger RNA; microRNA; monocytes; physiological regulation; transcription (genetics); transcription factors; translation (genetics); tumor necrosis factor-alpha
Abstract:: ... IL-10 is a potent anti-inflammatory molecule that, in phagocytes, negatively targets cytokine expression at transcriptional and posttranscriptional levels. Posttranscriptional checkpoints also represent the specific target of a recently discovered, evolutionary conserved class of small silencing RNAs known as “microRNAs” (miRNAs), which display the peculiar function of negatively regulating mRNA p ...
DOI:: 10.1073/pnas.1209100109
PubMed:: 23071313
PubMed Central:: PMC3494907
: https://doi.org/10.1073/pnas.1209100109

16. A 12-week double-blind randomized clinical trial of vitamin D₃ supplementation on body fat mass in healthy overweight and obese women

Author:: Amin Salehpour; Farhad Hosseinpanah; Farzad Shidfar; Mohammadreza Vafa; Maryam Razaghi; Sahar Dehghani; Anahita Hoshiarrad; Mahmoodreza Gohari
Source:: Nutrition journal 2012 v.11 no.1 pp. 553
ISSN:: 1475-2891
Subject:: body fat; body mass index; cholecalciferol; clinical trials; lactose; obesity; placebos; waist; waist circumference; women
Abstract:: ... BACKGROUND: Vitamin D concentrations are linked to body composition indices, particularly body fat mass. Relationships between hypovitaminosis D and obesity, described by both BMI and waist circumference, have been mentioned. We have investigated the effect of a 12-week vitamin D3 supplementation on anthropometric indices in healthy overweight and obese women. METHODS: In a double-blind, randomize ...
DOI:: 10.1186/1475-2891-11-78
PubMed:: 22998754
PubMed Central:: PMC3514135
: https://doi.org/10.1186/1475-2891-11-78

17. 14-3-3 Proteins SGF14c and SGF14l Play Critical Roles during Soybean Nodulation

Author:: Osman Radwan; Xia Wu; Manjula Govindarajulu; Marc Libault; David J. Neece; Man-Ho Oh; R. Howard Berg; Gary Stacey; Christopher G. Taylor; Steven C. Huber; Steven J. Clough
Source:: Plant physiology 2012 v.160 no.4 pp. 2125-2136
ISSN:: 0032-0889
Subject:: Bradyrhizobium japonicum; Glycine max; RNA interference; Rhizobium rhizogenes; cytoplasm; developmental stages; early development; genes; messenger RNA; nodulation; phenotype; proteins; proteomics; reverse transcriptase polymerase chain reaction; roots; soybeans; transcription (genetics); transcriptomics; transmission electron microscopy
Abstract:: ... The soybean (Glycine max) genome contains 18 members of the 14-3-3 protein family, but little is known about their association with specific phenotypes. Here, we report that the Glyma0529080 Soybean G-box Factor 14-3-3c (SGF14c) and Glyma08g12220 (SGF14l) genes, encoding 14-3-3 proteins, appear to play essential roles in soybean nodulation. Quantitative reverse transcription-polymerase chain react ...
DOI:: 10.1104/pp.112.207027
PubMed:: 23060368
PubMed Central:: PMC3510136
: https://doi.org/10.1104/pp.112.207027

18. 14-3-3 fusion oncogenes in high-grade endometrial stromal sarcoma

Author:: Cheng-Han Lee; Wen-Bin Ou; Adrian Mariño-Enriquez; Meijun Zhu; Mark Mayeda; Yuexiang Wang; Xiangqian Guo; Alayne L. Brunner; Frédéric Amant; Christopher A. French; Robert B. West; Jessica N. McAlpine; C. Blake Gilks; Michael B. Yaffe; Leah M. Prentice; Andrew McPherson; Steven J. M. Jones; Marco A. Marra; Sohrab P. Shah; Matt van de Rijn; David G. Huntsman; Paola Dal Cin; Maria Debiec-Rychter; Marisa R. Nucci; Jonathan A. Fletcher
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.3 pp. 929-934
ISSN:: 0027-8424
Subject:: cell growth; cytogenetics; disease course; exons; fluorescence in situ hybridization; gene expression; gene fusion; nuclear localization signals; oncogenes; protein synthesis; proteins; sarcoma; sequence analysis; small interfering RNA
Abstract:: ... 14-3-3 proteins are ubiquitously expressed regulators of various cellular functions, including proliferation, metabolism, and differentiation, and altered 14-3-3 expression is associated with development and progression of cancer. We report a transforming 14-3-3 oncoprotein, which we identified through conventional cytogenetics and whole-transcriptome sequencing analysis as a highly recurrent gene ...
DOI:: 10.1073/pnas.1115528109
PubMed:: 22223660
PubMed Central:: PMC3271913
: https://doi.org/10.1073/pnas.1115528109

19. 14-3-3 proteins act as scaffolds for GmMYB62 and GmMYB176 and regulate their intracellular localization in soybean

Author:: Xuyan Li; Sangeeta Dhaubhadel
Source:: Plant signaling & behavior 2012 v.7 no.8 pp. 965-968
ISSN:: 1559-2324
Subject:: Fabaceae; biosynthesis; clinical trials; food intake; gene expression; human health; isoflavonoids; nitrogen fixation; proteins; soybeans; stress tolerance
Abstract:: ... Isoflavonoids are plant natural compounds predominantly found in leguminous plant. They play important functions in both nitrogen fixation and stress resistance. Many clinical studies have linked dietary intake of isoflavonoids to human health benefits. Binding of 14-3-3 proteins to GmMYB176, an isoflavonoid regulator, modulates expression of key isoflavonoids gene expression and its biosynthesis. ...
DOI:: 10.4161/psb.20940
PubMed:: 22836494
PubMed Central:: PMC3474696
: https://doi.org/10.4161/psb.20940

20. The 14-3-3 proteins of Arabidopsis regulate root growth and chloroplast development as components of the photosensory system

Author:: John D. Mayfield; Anna-Lisa Paul; Robert J. Ferl
Source:: Journal of experimental botany 2012 v.63 no.8 pp. 3061-3070
ISSN:: 0022-0957
Subject:: Arabidopsis; growth and development; light intensity; mutants; protein binding; proteins; red light; root growth; roots; transfer DNA
Abstract:: ... The 14-3-3 proteins specifically bind a number of client proteins to influence important pathways, including flowering timing via the photosensory system. For instance, 14-3-3 proteins influence the photosensory system through interactions with Constans (CO) protein. 14-3-3 associations with the photosensory system were further studied in this investigation using 14-3-3 T-DNA insertion mutants to ...
DOI:: 10.1093/jxb/ers022
PubMed:: 22378945
PubMed Central:: PMC3350920
: https://doi.org/10.1093/jxb/ers022

21. 15N-Labeled Brain Enables Quantification of Proteome and Phosphoproteome in Cultured Primary Neurons

Author:: Liao Lujian; Sando Richard C.; Farnum John B.; Vanderklish Peter W.; Maximov Anton; Yates John R.
Source:: Journal of Proteome Research 2012 v.11 no.2 pp. 1341-1353
ISSN:: 1535-3907
Subject:: brain; models; neurons; phosphopeptides; phosphorylation; proteins; proteome; proteomics; quantitative analysis; stable isotopes; synapse; synaptic transmission
Abstract:: ... Terminally differentiated primary cells represent a valuable in vitro model to study signaling events associated within a specific tissue. Quantitative proteomic methods using metabolic labeling in primary cells encounter labeling efficiency issues hindering the use of these cells. Here we developed a method to quantify the proteome and phosphoproteome of cultured neurons using ¹⁵N-labeled brain t ...
DOI:: 10.1021/pr200987h
PubMed:: 22070516
PubMed Central:: PMC3279198
: https://doi.org/10.1021/pr200987h

22. 15N-Labeled Full-Length Apolipoprotein E4 as an Internal Standard for Mass Spectrometry Quantification of Apolipoprotein E Isoforms

Author:: Wang Meiyao; Chen Junjun; Turko Illarion V.
Source:: Analytical chemistry 2012 v.84 no.19 pp. 8340-8344
ISSN:: 1520-6882
Subject:: Alzheimer disease; alleles; amino acids; apolipoprotein E4; brain; genotype; humans; mass spectrometry; quantitative analysis; risk factors
Abstract:: ... Apolipoprotein E isoforms (apoE2, apoE3, and apoE4) affect the likelihood of developing Alzheimer’s disease (AD), with the apoE4 isoform being a major risk factor. However, the underlying mechanism remains to be determined. ApoE isoforms vary by a single amino acid change, and it is a challenge to distinguish them on the protein level. We developed a mass spectrometry-based quantitative method uti ...
DOI:: 10.1021/ac3018873
PubMed:: 22931427
PubMed Central:: PMC4038910
: https://doi.org/10.1021/ac3018873

23. 17 novel polymorphic microsatellite markers for the giant water bug, Abedus herberti (Belostomatidae)

Author:: T. S. Daly-Engel; R. L. Smith; D. S. Finn; M. E. Knoderbane; I. C. Phillipsen; D. A. Lytle
Source:: Conservation genetics resources 2012 v.4 no.4 pp. 979-981
ISSN:: 1877-7252
Subject:: Lethocerus americanus; alleles; aquatic habitat; climate change; evolution; groundwater; insects; loci; mating systems; microsatellite repeats
Abstract:: ... The giant water bug (Abedus herberti) is a large flightless insect that is a keystone predator in aridland aquatic habitats. Extended droughts, possibly due to climate change and groundwater pumping, are causing once-perennial aquatic habitats to dry, resulting in serious conservation concern for some populations. A. herberti also exhibits exclusive male parental care, which has made it a model or ...
DOI:: 10.1007/s12686-012-9687-5
PubMed:: 24077753
PubMed Central:: PMC3784353
: https://doi.org/10.1007/s12686-012-9687-5

24. 1H Dynamic Nuclear Polarization Based on an Endogenous Radical

Author:: Maly Thorsten; Cui Dongtao; Griffin Robert G.; Miller Anne-Frances
Source:: Journal of physical chemistry 2012 v.116 no.24 pp. 7055-7065
ISSN:: 1520-6106
Subject:: flavins; free radicals; nuclear magnetic resonance spectroscopy; organelles; physical chemistry; proteins
Abstract:: ... We demonstrate a 15-fold enhancement of solid-state NMR signals via dynamic nuclear polarization (DNP) based on a stable, naturally occurring radical in a protein: the flavin mononucleotide (FMN) semiquinone of flavodoxin. The line width of flavodoxin’s EPR signal suggests that the dominant DNP mechanism is the solid effect, consistent with the field-dependent DNP enhancement profile. The magnitud ...
DOI:: 10.1021/jp300539j
PubMed:: 22472179
PubMed Central:: PMC6010065
: https://doi.org/10.1021/jp300539j

25. 1α,25-Dihydroxyvitamin D₃ and its analog, 2-methylene-19-nor-(20S)-1α,25-dihydroxyvitamin D₃ (2MD), suppress intraocular pressure in non-human primates

Author:: Galina D. Kutuzova; B’Ann T. Gabelt; Julie A. Kiland; Elizabeth A. Hennes-Beann; Paul L. Kaufman; Hector F. DeLuca
Source:: Archives of biochemistry and biophysics 2012 v.518 no.1 pp. 53-60
ISSN:: 0003-9861
Subject:: Primates; drainage; genes; glaucoma; hypertension; microarray technology; people; risk factors; therapeutics
Abstract:: ... Ocular hypertension is the greatest known risk factor for glaucoma that affects an estimated 70million people worldwide. Lowering intraocular pressure (IOP) remains the mainstay of therapy in the management of glaucoma. By means of microarray analysis, we have discovered that 1α,25-dihydroxyvitamin D₃ (1α,25-(OH)₂D₃) regulates genes that are known to be involved in the determination of intraocular ...
DOI:: 10.1016/j.abb.2011.10.022
PubMed:: 22198282
PubMed Central:: PMC3390938
: https://doi.org/10.1016/j.abb.2011.10.022

26. 1’-Acetoxychavicol acetate inhibits growth of human oral carcinoma xenograft in mice and potentiates cisplatin effect via proinflammatory microenvironment alterations

Author:: Lionel LA In; Norhafiza M Arshad; Halijah Ibrahim; Mohamad Nurul Azmi; Khalijah Awang; Noor Hasima Nagoor
Source:: BMC complementary and alternative medicine 2012 v.12 no.1 pp. 179
ISSN:: 1472-6882
Subject:: DNA fragmentation; Western blotting; acetates; alternative medicine; apoptosis; biomarkers; body weight changes; cisplatin; clinical trials; cyclins; cytotoxicity; genes; ginger; humans; mechanism of action; mice; mouth neoplasms; squamous cell carcinoma; survival rate; transcription factor NF-kappa B
Abstract:: ... BACKGROUND: Oral cancers although preventable, possess a low five-year survival rate which has remained unchanged over the past three decades. In an attempt to find a more safe, affordable and effective treatment option, we describe here the use of 1’S-1’-acetoxychavicol acetate (ACA), a component of Malaysian ginger traditionally used for various medicinal purposes. METHODS: Whether ACA can inhib ...
DOI:: 10.1186/1472-6882-12-179
PubMed:: 23043547
PubMed Central:: PMC3517351
: https://doi.org/10.1186/1472-6882-12-179

27. 2,2,2-Trifluoroethyl 6-thio-β-d-glucopyranoside as a selective tag for cysteines in proteins

Author:: Richard F.G. Fröhlich; Evelyne Schrank; Klaus Zangger
Source:: Carbohydrate research 2012 v.361 no. pp. 100-104
ISSN:: 0008-6215
Subject:: cysteine; fluorine; glucose; glycosylation; microwave treatment; nuclear magnetic resonance spectroscopy; risk; solvents; thiols; transactivators
Abstract:: ... A synthetic route to a trifluoromethyl and thiol containing glucose derivative (2,2,2-trifluoroethyl 6-thio-β-d-glucopyranoside) is presented, which is based on microwave-assisted Fischer glycosylation under increased pressure. This water-soluble, neutral thiol-compound can be used to selectively introduce a fluorine probe for ¹⁹F NMR spectroscopy on cysteines in proteins. It can be attached under ...
DOI:: 10.1016/j.carres.2012.08.010
PubMed:: 23000216
PubMed Central:: PMC4067056
: https://doi.org/10.1016/j.carres.2012.08.010

28. 2,2′,3,5′,6-Pentachlorobiphenyl (PCB 95) and Its Hydroxylated Metabolites Are Enantiomerically Enriched in Female Mice

Author:: Kania-Korwel Izabela; Barnhart Christopher D.; Stamou Marianna; Truong Kim M.; El-Komy Mohammed H. M. E.; Lein Pamela J.; Veng-Pedersen Peter; Lehmler Hans-Joachim
Source:: Environmental Science & Technology 2012 v.46 no.20 pp. 11393-11401
ISSN:: 1520-5851
Subject:: adults; blood; brain; dose response; females; liver; metabolites; mice; neurotoxicity; polychlorinated biphenyls; subchronic exposure; tissues
Abstract:: ... Epidemiological and laboratory studies link polychlorinated biphenyls and their metabolites to adverse neurodevelopmental outcomes. Several neurotoxic PCB congeners are chiral and undergo enantiomeric enrichment in mammalian species, which may modulate PCB developmental neurotoxicity. This study measures levels and enantiomeric enrichment of PCB 95 and its hydroxylated metabolites (OH-PCBs) in adu ...
DOI:: 10.1021/es302810t
PubMed:: 22974126
PubMed Central:: PMC3475189
: https://doi.org/10.1021/es302810t

29. 2,3-Hexanediols as Sex Attractants and a Female-produced Sex Pheromone for Cerambycid Beetles in the Prionine Genus Tragosoma

Author:: Ann M. Ray; James D. Barbour; J. Steven McElfresh; Jardel A. Moreira; Ian Swift; Ian M. Wright; Alenka Žunič; Robert F. Mitchell; Elizabeth E. Graham; Ronald L. Alten; Jocelyn G. Millar; Lawrence M. Hanks
Source:: Journal of chemical ecology 2012 v.38 no.9 pp. 1151-1158
ISSN:: 0098-0331
Subject:: Cerambycidae; aggregation pheromones; bioassays; females; fresh produce; males; pheromone blends; pheromone glands; sex attractants; sex pheromones; sexual dimorphism; California
Abstract:: ... Recent work suggests that closely related cerambycid species often share pheromone components, or even produce pheromone blends of identical composition. However, little is known of the pheromones of species in the subfamily Prioninae. During field bioassays in California, males of three species in the prionine genus Tragosoma were attracted to 2,3-hexanediols, common components of male-produced a ...
DOI:: 10.1007/s10886-012-0181-z
PubMed:: 22923142
PubMed Central:: PMC3808253
: https://doi.org/10.1007/s10886-012-0181-z

30. 2-Hydroxyoleate, a nontoxic membrane binding anticancer drug, induces glioma cell differentiation and autophagy

Author:: Silvia TerÃ©s; Victoria LladÃ³; MÃ³nica Higuera; Gwendolyn BarcelÃ³-Coblijn; Maria Laura Martin; Maria AntÃ²nia Noguera-SalvÃ ; Amaia Marcilla-Etxenike; JosÃ© Manuel GarcÃa-Verdugo; Mario Soriano-Navarro; Carlos Saus; Ulises GÃ³mez-Pinedo; Xavier Busquets; Pablo V. EscribÃ¡
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.22 pp. 8489-8494
ISSN:: 0027-8424
Subject:: anticarcinogenic activity; antineoplastic agents; brain; cell death; cell differentiation; cytoplasm; lipid composition; mechanism of action; mitogen-activated protein kinase; neoplasms; neuroglia; oral administration; patients; plasma membrane; protein phosphorylation; protein transport; proteins; relapse; sphingomyelins; survival rate; toxicity
Abstract:: ... Despite recent advances in the development of new cancer therapies, the treatment options for glioma remain limited, and the survival rate of patients has changed little over the past three decades. Here, we show that 2-hydroxyoleic acid (2OHOA) induces differentiation and autophagy of human glioma cells. Compared to the current reference drug for this condition, temozolomide (TMZ), 2OHOA combated ...
DOI:: 10.1073/pnas.1118349109
PubMed:: 22586083
PubMed Central:: PMC3365159
: https://doi.org/10.1073/pnas.1118349109

31. 2-Methoxyestradiol inhibits experimental autoimmune encephalomyelitis through suppression of immune cell activation

Author:: Gordon S. Duncan; Dirk Brenner; Michael W. Tusche; Anne Brüstle; Christiane B. Knobbe; Andrew J. Elia; Thomas Mock; Mark R. Bray; Peter H. Krammer; Tak W. Mak
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.51 pp. 21034-21039
ISSN:: 0027-8424
Subject:: T-lymphocytes; animal models; cytokines; dose response; drugs; encephalitis; estradiol; humans; lymphocyte proliferation; metabolites; mice; neoplasms; rheumatoid arthritis; sclerosis; transcription (genetics); transcription factor NF-kappa B
Abstract:: ... The endogenous metabolite of estradiol, 2-Methoxyestradiol (2ME2), is an antimitotic and antiangiogenic cancer drug candidate that also exhibits disease-modifying activity in animal models of rheumatoid arthritis (RA). We found that 2ME2 dramatically suppresses development of mouse experimental autoimmune encephalomyelitis (EAE), a rodent model of multiple sclerosis (MS). 2ME2 inhibits in vitro ly ...
DOI:: 10.1073/pnas.1215558110
PubMed:: 23213242
PubMed Central:: PMC3529073
: https://doi.org/10.1073/pnas.1215558110

32. The 2009 pandemic H1N1 virus induces anti-neuraminidase (NA) antibodies that cross-react with the NA of H5N1 viruses in ferrets

Author:: Zhongying Chen; Lomi Kim; Kanta Subbarao; Hong Jin
Source:: Vaccine 2012 v.30 no.15 pp. 2516-2522
ISSN:: 0264-410X
Subject:: Influenza A virus; amino acids; antibodies; birds; cross reaction; ferrets; humans; influenza; pandemic; proteins; sialidase; vaccines; viruses
Abstract:: ... A miniaturized neuraminidase inhibition (NI) assay using HA-mismatched H6 reassortant viruses was performed to examine the neuraminidase (NA)-specific antibody response in ferrets immunized with live-attenuated influenza vaccine (LAIV) strains. The strains tested possessed different NAs derived from seasonal H1N1 and H3N2, 2009 pandemic H1N1, and the highly pathogenic influenza H5N1 virus. The ant ...
DOI:: 10.1016/j.vaccine.2012.01.090
PubMed:: 22330124
PubMed Central:: PMC4792253
: https://doi.org/10.1016/j.vaccine.2012.01.090

33. A 21-day Daniel fast with or without krill oil supplementation improves anthropometric parameters and the cardiometabolic profile in men and women

Author:: John F Trepanowski; Mohammad M Kabir; Rick J Alleman Jr; Richard J Bloomer
Source:: Nutrition & metabolism 2012 v.9 no.1 pp. 478
ISSN:: 1743-7075
Subject:: alcohols; animal products; blood; blood glucose; blood lipids; blood pressure; body mass index; body weight; caffeine; coconut oil; flavorings; flour; glucose; insulin; insulin resistance; krill; men; oxidative stress; placebos; preservatives; sweeteners; women
Abstract:: ... BACKGROUND: The Daniel Fast is a vegan diet that prohibits the consumption of animal products, refined foods, white flour, preservatives, additives, sweeteners, flavorings, caffeine, and alcohol. Following this dietary plan for 21 days has been demonstrated to improve blood pressure, LDL-C, and certain markers of oxidative stress, but it has also been shown to lower HDL-C. Krill oil supplementatio ...
DOI:: 10.1186/1743-7075-9-82
PubMed:: 22971786
PubMed Central:: PMC3517900
: https://doi.org/10.1186/1743-7075-9-82

34. The 21.5-kDa isoform of myelin basic protein has a non-traditional PY-nuclear-localization signal

Author:: Graham S.T. Smith; Lauren V. Seymour; Joan M. Boggs; George Harauz
Source:: Biochemical and biophysical research communications 2012 v.422 no.4 pp. 670-675
ISSN:: 0006-291X
Subject:: actin; arginine; central nervous system; fluorescence microscopy; fluorescent proteins; genes; lysine; myelin basic protein; myelin sheath; protein transport; site-directed mutagenesis; tubulin
Abstract:: ... The predominant 18.5-kDa classic myelin basic protein (MBP) is mainly responsible for compaction of the myelin sheath in the central nervous system, but is multifunctional, having numerous interactions with Ca²⁺-calmodulin, actin, tubulin, and SH3-domains, and can tether these proteins to a lipid membrane in vitro. The full-length 21.5-kDa MBP isoform has an additional 26 residues encoded by exon- ...
DOI:: 10.1016/j.bbrc.2012.05.051
PubMed:: 22609403
PubMed Central:: PMC3526657
: https://doi.org/10.1016/j.bbrc.2012.05.051

35. 24-Hour profiles of circulating ghrelin and peptide YY are inversely associated in normal weight premenopausal women

Author:: Brenna R. Hill; Mary Jane De Souza; David A. Wagstaff; Rino Sato; Nancy I. Williams
Source:: Peptides 2012 v.38 no.1 pp. 159-162
ISSN:: 0196-9781
Subject:: blood; body fat; body mass index; carbohydrates; food intake; ghrelin; humans; hypothalamus; models; peptide YY; premenopause; radioimmunoassays; secretion; statistical analysis; women
Abstract:: ... Peptide YY (PYY) and ghrelin (GHR) may modulate one another's actions within the hypothalamus. Peripheral infusion of PYY in humans acutely suppresses circulating concentrations of GHR. Whether an association between PYY and GHR exists in the peripheral circulation of humans over 24h is unknown. The purpose of this study was to determine if circulating concentrations of PYY and GHR were significan ...
DOI:: 10.1016/j.peptides.2012.08.017
PubMed:: 22954902
PubMed Central:: PMC3496067
: https://doi.org/10.1016/j.peptides.2012.08.017

36. 25-Hydroxyvitamin D Concentrations and In Vivo Insulin Sensitivity and β-Cell Function Relative to Insulin Sensitivity in Black and White Youth

Author:: Kumaravel Rajakumar; Javier de las Heras; SoJung Lee; Michael F. Holick; Silva A. Arslanian
Source:: Diabetes care 2012 v.35 no.3 pp. 627-633
ISSN:: 0149-5992
Subject:: Blacks; Whites; adipose tissue; adiposity; body mass index; body measurements; fasting; glucose; homeostasis; insulin; insulin resistance; secretion; vitamin D; volunteers; youth
Abstract:: ... OBJECTIVE: To examine the relationships between plasma 25-hydroxyvitamin D [25(OH)D] and in vivo insulin sensitivity and β-cell function relative to insulin sensitivity, disposition index (DI), in black and white youth. RESEARCH DESIGN AND METHODS: Plasma 25(OH)D concentrations were analyzed in banked specimens in healthy youth aged 8 to 18 years who had existing data on hyperinsulinemic-euglycemi ...
DOI:: 10.2337/dc11-1825
PubMed:: 22238280
PubMed Central:: PMC3322701
: https://doi.org/10.2337/dc11-1825

37. 25-Hydroxyvitamin D deficiency is associated with fatal stroke among whites but not blacks: The NHANES-III linked mortality files

Author:: Erin D. Michos; Jared P. Reis; Wendy S. Post; Pamela L. Lutsey; Rebecca F. Gottesman; Thomas H. Mosley; A. Richey Sharrett; Michal L. Melamed
Source:: Nutrition 2012 v.28 no.4 pp. 367-371
ISSN:: 0899-9007
Subject:: risk factors; regression analysis; confidence interval; socioeconomic status; Whites; stroke; vitamin deficiencies; Blacks; National Health and Nutrition Examination Survey; mortality; adults; United States
Abstract:: ... OBJECTIVE: Deficient 25-hydroxyvitamin D (25[OH]D) levels are associated with cardiovascular disease (CVD) events and mortality. 25(OH)D deficiency and stroke are more prevalent in blacks. We examined whether low 25(OH)D contributes to the excess risk of fatal stroke in blacks compared with whites. METHODS: The Third National Health and Nutrition Examination Survey, a probability sample of U.S. ci ...
DOI:: 10.1016/j.nut.2011.10.015
PubMed:: 22261577
PubMed Central:: PMC3304002
: https://doi.org/10.1016/j.nut.2011.10.015

38. 26S Proteasome regulation of Ankrd1/CARP in adult rat ventricular myocytes and human microvascular endothelial cells

Author:: Susan E. Samaras; Billy Chen; Stephen R. Koch; Douglas B. Sawyer; Chee Chew Lim; Jeffrey M. Davidson
Source:: Biochemical and biophysical research communications 2012 v.425 no.4 pp. 830-835
ISSN:: 0006-291X
Subject:: proteasome endopeptidase complex; messenger RNA; tissue repair; endothelial cells; humans; muscles; sarcomeres; proteolysis; rats; adults; half life
Abstract:: ... Ankyrin repeat domain 1 protein (Ankrd1), also known as cardiac ankyrin repeat protein (CARP), increases dramatically after tissue injury, and its overexpression improves aspects of wound healing. Reports that Ankrd1/CARP protein stability may affect cardiovascular organization, together with our findings that the protein is crucial to stability of the cardiomyocyte sarcomere and increased in woun ...
DOI:: 10.1016/j.bbrc.2012.07.162
PubMed:: 22892129
PubMed Central:: PMC3460693
: https://doi.org/10.1016/j.bbrc.2012.07.162

39. The 27–year decline of coral cover on the Great Barrier Reef and its causes

Author:: Glenn De’ath; Katharina E. Fabricius; Hugh Sweatman; Marji Puotinen
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.44 pp. 17995-17999
ISSN:: 0027-8424
Subject:: Acanthaster planci; bleaching; climatic factors; control methods; coral bleaching; coral reefs; corals; hurricanes; mortality; predation; surveys; temporal variation; time series analysis; water quality; Great Barrier Reef
Abstract:: ... The world’s coral reefs are being degraded, and the need to reduce local pressures to offset the effects of increasing global pressures is now widely recognized. This study investigates the spatial and temporal dynamics of coral cover, identifies the main drivers of coral mortality, and quantifies the rates of potential recovery of the Great Barrier Reef. Based on the world’s most extensive time s ...
DOI:: 10.1073/pnas.1208909109
PubMed:: 23027961
PubMed Central:: PMC3497744
: https://doi.org/10.1073/pnas.1208909109

40. A 29-mer site regulates transcription of the initiator gene as well as function of the replication origin of Vibrio cholerae chromosome II

Author:: Tatiana Venkova-Canova; Anik Saha; Dhruba K. Chattoraj
Source:: Plasmid 2012 v.67 no.2 pp. 102-110
ISSN:: 0147-619X
Subject:: Escherichia coli; Vibrio cholerae; binding sites; cell cycle; chromosomes; genes; methylation; plasmids; promoter regions; replication origin; transcription (genetics)
Abstract:: ... The region responsible for replication of Vibrio cholerae chromosome II (chrII) resembles those of plasmids that have repeated initiator binding sites (iterons) and an autorepressed initiator gene. ChrII has additional features: Its iterons require full methylation for initiator (RctB) binding, which makes them inactive for a part of the cell cycle when they are hemi-methylated. RctB also binds to ...
DOI:: 10.1016/j.plasmid.2011.12.009
PubMed:: 22248922
PubMed Central:: PMC3319240
: https://doi.org/10.1016/j.plasmid.2011.12.009

41. The 2b proteins of Cucumber mosaic virus generally have the potential to differentially induce necrosis on Arabidopsis

Author:: Chikara Masuta; Jun-ichi Inaba; Hanako Shimura
Source:: Plant signaling & behavior 2012 v.7 no.1 pp. 43-45
ISSN:: 1559-2324
Subject:: Arabidopsis; Cucumber mosaic virus; RNA interference; catalase; enzyme activity; hydrogen peroxide; necrosis; proteins; sequence homology; virulent strains
Abstract:: ... Plant viral symptoms are rarely explained by direct molecular interaction between a viral protein and a host factor, but rather understood as a consequence of arms race between host RNA silencing and viral silencing suppressors. However, we have recently demonstrated that the 2b protein (2b) of Cucumber mosaic virus (CMV) HL strain could bind to Arabidopsis catalase that is important in scavenging ...
DOI:: 10.4161/psb.7.1.18526
PubMed:: 22301966
PubMed Central:: PMC3357366
: https://doi.org/10.4161/psb.7.1.18526

42. 3'-UTR-located inverted Alu repeats facilitate mRNA translational repression and stress granule accumulation

Author:: Terry Fitzpatrick; Sui Huang
Source:: Nucleus 2012 v.3 no.4 pp. 359-369
ISSN:: 1949-1042
Subject:: gene expression; humans; mutational analysis; nucleotide sequences; reporter genes
Abstract:: ... Alu repeats within human genes may potentially alter gene expression. Here, we show that 3′-UTR-located inverted Alu repeats significantly reduce expression of an AcGFP reporter gene. Mutational analysis demonstrates that the secondary structure, but not the primary nucleotide sequence, of the inverted Alu repeats is critical for repression. The expression levels and nucleocytoplasmic distribution ...
DOI:: 10.4161/nucl.20827
PubMed:: 22688648
PubMed Central:: PMC3679280
: https://doi.org/10.4161/nucl.20827

43. 3-D and Quasi-2-D Discrete Element Modeling of Grain Commingling in a Bucket Elevator Boot System

Author:: Boac, J.M.; Casada, M.E.; Maghirang, R.G.; Harner, J.P. III
Source:: Transactions of the ASABE 2012 v.55 no.2 pp. 659-672
ISSN:: 2151-0032
Subject:: buckets; elevators; grain elevators; grains; mixing; prediction; purity; simulation models; soybeans
Abstract:: ... Unwanted grain commingling impedes new quality-based grain handling systems and has proven to be an expensive and time-consuming issue to study experimentally. Experimentally validated models may reduce the time and expense of studying grain commingling while providing additional insight into details of the particle flow. In this study, grain commingling in a pilot-scale bucket elevator boot was f ...
Handle:: 10113/54153

44. 3-Dimensional Imaging Modalities for Phenotyping Genetically Engineered Mice

Author:: K. A. Powell; D. Wilson
Source:: Veterinary pathology v.49 no.1 pp. 106-115
ISSN:: 1544-2217
Subject:: X-radiation; digital images; embryogenesis; fluorescence; image analysis; mice; microscopy; tomography; ultrasonics
Abstract:: ... A variety of 3-dimensional (3D) digital imaging modalities are available for whole-body assessment of genetically engineered mice: magnetic resonance microscopy (MRM), X-ray microcomputed tomography (microCT), optical projection tomography (OPT), episcopic and cryoimaging, and ultrasound biomicroscopy (UBM). Embryo and adult mouse phenotyping can be accomplished at microscopy or near microscopy sp ...
DOI:: 10.1177/0300985811429814
PubMed:: 22146851
PubMed Central:: PMC3857693
: https://doi.org/10.1177/0300985811429814

45. 3-Helix Micelles Stabilized by Polymer Springs

Author:: Dong He; Shu Jessica Y.; Dube Nikhil; Ma Yufei; Tirrell Matthew V.; Downing Kenneth H.; Xu Ting
Source:: Journal of the American Chemical Society 2012 v.134 no.28 pp. 11807-11814
ISSN:: 1520-5126
Subject:: chemical bonding; colloids; deformation; desorption; energy; hydrophilicity; micelles; nanocarriers; particle size; polyethylene glycol
Abstract:: ... Despite increasing demands to employ amphiphilic micelles as nanocarriers and nanoreactors, it remains a significant challenge to simultaneously reduce the particle size and enhance the particle stability. Complementary to covalent chemical bonding and attractive intermolecular interactions, entropic repulsion can be incorporated by rational design in the headgroup of an amphiphile to generate sma ...
DOI:: 10.1021/ja3048128
PubMed:: 22731391
PubMed Central:: PMC4186246
: https://doi.org/10.1021/ja3048128

46. 3-Picolyl Azide Adenine Dinucleotide as a Probe of Femtosecond to Picosecond Enzyme Dynamics

Author:: Dutta Samrat; Li Yun-Liang; Rock William; Houtman Jon C. D.; Kohen Amnon; Cheatum Christopher M.
Source:: Journal of physical chemistry 2012 v.116 no.1 pp. 542-548
ISSN:: 1520-5207
Subject:: absorption; active sites; adenine; aqueous solutions; formate dehydrogenase; hydrogen bonding; infrared spectroscopy
Abstract:: ... Functionally relevant femtosecond to picosecond dynamics in enzyme active sites can be difficult to measure because of a lack of spectroscopic probes that can be located in the active site without altering the behavior of the enzyme. We have developed a new NAD⁺ analog 3-Picolyl Azide Adenine Dinucleotide (PAAD⁺), which has the potential to be a general spectroscopic probe for NAD-dependent enzyme ...
DOI:: 10.1021/jp208677u
PubMed:: 22126535
PubMed Central:: PMC3260785
: https://doi.org/10.1021/jp208677u

47. IL- 33 is processed into mature bioactive forms by neutrophil elastase and cathepsin G

Author:: Emma Lefrançais; Stephane Roga; Violette Gautier; Anne Gonzalez-de-Peredo; Bernard Monsarrat; Jean-Philippe Girard; Corinne Cayrol
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.5 pp. 1673-1678
ISSN:: 0027-8424
Subject:: asthma; basophils; bioactive properties; cardiovascular diseases; caspase-1; cathepsin G; colitis; elastase; eosinophils; humans; inflammation; interleukin-1; interleukin-18; lymphocytes; mast cells; mice; neutrophils; rheumatoid arthritis
Abstract:: ... Interleukin-33 (IL-33) (NF-HEV) is a chromatin-associated nuclear cytokine from the IL-1 family, which has been linked to important diseases, including asthma, rheumatoid arthritis, ulcerative colitis, and cardiovascular diseases. IL-33 signals through the ST2 receptor and drives cytokine production in type 2 innate lymphoid cells (ILCs) (natural helper cells, nuocytes), T-helper (Th)2 lymphocytes ...
DOI:: 10.1073/pnas.1115884109
PubMed:: 22307629
PubMed Central:: PMC3277172
: https://doi.org/10.1073/pnas.1115884109

48. IL- 38 binds to the IL-36 receptor and has biological effects on immune cells similar to IL-36 receptor antagonist

Author:: Frank L. van de Veerdonk; Angela K. Stoeckman; Gouping Wu; Aaron N. Boeckermann; Tania Azam; Mihai G. Netea; Leo A. B. Joosten; Jos W. M. van der Meer; Ruyi Hao; Vassili Kalabokis; Charles A. Dinarello
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.8 pp. 3001-3005
ISSN:: 0027-8424
Subject:: Candida albicans; amino acids; antagonists; dendritic cells; humans; interleukin-1; interleukin-6; interleukin-8; mononuclear leukocytes
Abstract:: ... The functional role of IL-1 family member 10, recently renamed IL-38, remains unknown. In the present study we aimed to elucidate the biological function of IL-38 and to identify its receptor. Heat-killed Candida albicans was used to stimulate memory T-lymphocyte cytokine production in freshly obtained human peripheral blood mononuclear cells from healthy subjects. The addition of recombinant IL-3 ...
DOI:: 10.1073/pnas.1121534109
PubMed:: 22315422
PubMed Central:: PMC3286950
: https://doi.org/10.1073/pnas.1121534109

49. 3D cell entrapment in crosslinked thiolated gelatin-poly(ethylene glycol) diacrylate hydrogels

Author:: Yao Fu; Kedi Xu; Xiaoxiang Zheng; Alan J. Giacomin; Adam W. Mix; Weiyuan J. Kao
Source:: Biomaterials 2012 v.33 no.1 pp. 48-58
ISSN:: 0142-9612
Subject:: crosslinking; cysteine; ethylene glycol; fibroblasts; gelatin; hydrocolloids; mechanical properties; physicochemical properties; thiols; tissue engineering; water content
Abstract:: ... The combined use of natural ECM components and synthetic materials offers an attractive alternative to fabricate hydrogel-based tissue engineering scaffolds to study cell-matrix interactions in three-dimensions (3D). A facile method was developed to modify gelatin with cysteine via a bifunctional PEG linker, thus introducing free thiol groups to gelatin chains. A covalently crosslinked gelatin hyd ...
DOI:: 10.1016/j.biomaterials.2011.09.031
PubMed:: 21955690
PubMed Central:: PMC3282186
: https://doi.org/10.1016/j.biomaterials.2011.09.031

50. 3D imaging and mechanical modeling of helical buckling in Medicago truncatula plant roots

Author:: Jesse L. Silverberg; Roslyn D. Noar; Michael S. Packer; Maria J. Harrison; Christopher L. Henley; Itai Cohen; Sharon J. Gerbode
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.42 pp. 16794-16799
ISSN:: 0027-8424
Subject:: Medicago truncatula; culture media; deformation; gels; hydrocolloids; image analysis; mathematical models; root growth; roots
Abstract:: ... We study the primary root growth of wild-type Medicago truncatula plants in heterogeneous environments using 3D time-lapse imaging. The growth medium is a transparent hydrogel consisting of a stiff lower layer and a compliant upper layer. We find that the roots deform into a helical shape just above the gel layer interface before penetrating into the lower layer. This geometry is interpreted as a ...
DOI:: 10.1073/pnas.1209287109
PubMed:: 23010923
PubMed Central:: PMC3479456
: https://doi.org/10.1073/pnas.1209287109

51. A 3D localized surface plasmon resonance biosensor for the study of trivalent arsenic binding to the ArsA ATPase

Author:: Chang Liu; Vittoria Balsamo; Dali Sun; Melodie Naja; Xuemei Wang; Barry Rosen; Chen-Zhong Li
Source:: Biosensors & bioelectronics 2012 v.38 no.1 pp. 19-26
ISSN:: 0956-5663
Subject:: Escherichia coli; acrylic acid; adenosine triphosphate; adenosinetriphosphatase; arsenic; arsenites; binding sites; biosensors; magnesium; nanogold; operon; plasmids; protein subunits; proteins; surface plasmon resonance
Abstract:: ... A self-assembled 3D hydrogel–nanoparticle composite integrated surface plasmon resonance (SPR) sensor is reported here. The novel assembled substrate was developed by means of a surface mediated radical co-polymerization process to obtain a highly sensitive hydrogel-based thin film that possesses specific binding sites for target analytes. Initially, amino group modified gold nanoparticles (AuNPs) ...
DOI:: 10.1016/j.bios.2012.04.026
PubMed:: 22658909
PubMed Central:: PMC3715140
: https://doi.org/10.1016/j.bios.2012.04.026

52. 3D superhydrophobic electrospun meshes as reinforcement materials for sustained local drug delivery against colorectal cancer cells

Author:: Stefan T. Yohe; Victoria L.M. Herrera; Yolonda L. Colson; Mark W. Grinstaff
Source:: Journal of controlled release 2012 v.162 no.1 pp. 92-101
ISSN:: 0168-3659
Subject:: air; antineoplastic agents; colorectal neoplasms; cytotoxicity; drug therapy; glycerol; humans; hydrophobicity; mechanical properties; neoplasm cells; polymers; ultrasonics
Abstract:: ... In this work we expand upon a recently reported local drug delivery device, where air is used as a degradable component of our material to control drug release (J. Am. Chem. Soc. 2012, 134, 2016–2019). We consider its potential use as a drug loaded strip to provide both mechanical stability to the anastomosis, and as a means to release drug locally over prolonged periods for prevention of locoregi ...
DOI:: 10.1016/j.jconrel.2012.05.047
PubMed:: 22684120
PubMed Central:: PMC3878861
: https://doi.org/10.1016/j.jconrel.2012.05.047

53. 3β-Methoxy-pregnenolone (MAP4343) as an innovative therapeutic approach for depressive disorders

Author:: Massimiliano Bianchi; Etienne-Emile Baulieu
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.5 pp. 1713-1718
ISSN:: 0027-8424
Subject:: amygdala; antidepressants; cortex; hippocampus; mechanism of action; models; pathogenesis; rats; tubulin
Abstract:: ... Emerging evidence suggests that the pathogenesis of depressive disorders (DDs) is associated with neuronal abnormalities in brain microtubule function, including changes in α-tubulin isoforms. Currently available antidepressant drugs may act by rescuing these alterations, but only after long-term treatment explaining their delayed therapeutic efficacy. The microtubule associated protein type-2 (MA ...
DOI:: 10.1073/pnas.1121485109
PubMed:: 22307636
PubMed Central:: PMC3277154
: https://doi.org/10.1073/pnas.1121485109

54. 3′ Phosphatase activity toward phosphatidylinositol 3,4-bisphosphate [PI(3,4)P₂] by voltage-sensing phosphatase (VSP)

Author:: Tatsuki Kurokawa; Shunsuke Takasuga; Souhei Sakata; Shinji Yamaguchi; Shigeo Horie; Koichi J. Homma; Takehiko Sasaki; Yasushi Okamura
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.25 pp. 10089-10094
ISSN:: 0027-8424
Subject:: Ciona intestinalis; amphibians; chromosomes; confocal microscopy; fluorescence; humans; in vitro studies; inositols; lipids; membrane potential; monitoring; oocytes; phosphates; pleckstrin; radiolabeling; sequence homology; substrate specificity
Abstract:: ... Voltage-sensing phosphatases (VSPs) consist of a voltage-sensor domain and a cytoplasmic region with remarkable sequence similarity to phosphatase and tensin homolog deleted on chromosome 10 (PTEN), a tumor suppressor phosphatase. VSPs dephosphorylate the 5′ position of the inositol ring of both phosphatidylinositol 3,4,5-trisphosphate [PI(3,4,5)P ₃] and phosphatidylinositol 4,5-bisphosphate [PI(4 ...
DOI:: 10.1073/pnas.1203799109
PubMed:: 22645351
PubMed Central:: PMC3382541
: https://doi.org/10.1073/pnas.1203799109

55. 4-Hydroxynonenal differentially regulates adiponectin gene expression and secretion via activating PPARγ and accelerating ubiquitin–proteasome degradation

Author:: Zhigang Wang; Xiaobing Dou; Dongfang Gu; Chen Shen; Tong Yao; Van Nguyen; Carol Braunschweig; Zhenyuan Song
Source:: Molecular and cellular endocrinology 2012 v.349 no.2 pp. 222-231
ISSN:: 0303-7207
Subject:: adipocytes; adiponectin; adipose tissue; antagonists; antioxidants; betaine; cycloheximide; dose response; gene expression; high fat diet; insulin resistance; lipid peroxidation; metabolic diseases; mice; obesity; oxidative stress; precipitin tests; proteasome endopeptidase complex; protein degradation; secretion
Abstract:: ... Although well-established, the underlying mechanisms involved in obesity-related plasma adiponectin decline remain elusive. Oxidative stress is associated with obesity and insulin resistance and considered to contribute to the progression toward obesity-related metabolic disorders. In this study, we investigated the effects of 4-hydroxynonenal (4-HNE), the most abundant lipid peroxidation end prod ...
DOI:: 10.1016/j.mce.2011.10.027
PubMed:: 22085560
PubMed Central:: PMC3594100
: https://doi.org/10.1016/j.mce.2011.10.027

56. 4-O-methylation of glucuronic acid in Arabidopsis glucuronoxylan is catalyzed by a domain of unknown function family 579 protein

Author:: Breeanna R. Urbanowicz; Maria J. Peña; Supriya Ratnaparkhe; Utku Avci; Jason Backe; Heather F. Steet; Marcus Foston; Hongjia Li; Malcolm A. O’Neill; Arthur J. Ragauskas; Alan G. Darvill; Charles Wyman; Harry J. Gilbert; William S. York
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.35 pp. 14253-14258
ISSN:: 0027-8424
Subject:: physical properties; glucuronic acid; Arabidopsis; cobalt; proteins; xylan; biopolymers; cell walls; biochemical mechanisms; hot water treatment; lignin; methyltransferases; Golgi apparatus
Abstract:: ... The hemicellulose 4- O -methyl glucuronoxylan is one of the principle components present in the secondary cell walls of eudicotyledonous plants. However, the biochemical mechanisms leading to the formation of this polysaccharide and the effects of modulating its structure on the physical properties of the cell wall are poorly understood. We have identified and functionally characterized an Arabido ...
DOI:: 10.1073/pnas.1208097109
PubMed:: 22893684
PubMed Central:: PMC3435161
: https://doi.org/10.1073/pnas.1208097109

57. 470-Million-year-old black corals from China

Author:: Andrzej Baliński; Yuanlin Sun; Jerzy Dzik
Source:: Naturwissenschaften 2012 v.99 no.8 pp. 645-653
ISSN:: 0028-1042
Subject:: Anthozoa; chitin; corals; fossils; phylogeny; prediction; China
Abstract:: ... Phosphatic (possibly secondarily phosphatised) remains of antipatharian coralla, previously unknown in the fossil record, occur abundantly in the early Ordovician Fenxiang Formation in the Hubei Province, southern China. Probably two species (and genera) are represented, which differ in spinosity of branches. The more spinose one, Sinopathes reptans, has its lateral spines bearing regular, longitu ...
DOI:: 10.1007/s00114-012-0947-8
PubMed:: 22790835
PubMed Central:: PMC3407557
: https://doi.org/10.1007/s00114-012-0947-8

58. 5-Carboxylcytosine is localized to euchromatic regions in the nuclei of follicular cells in axolotl ovary

Author:: Anthony Alioui; Lee M. Wheldon; Abdulkadir Abakir; Zoltan Ferjentsik; Andrew D. Johnson; Alexey Ruzov
Source:: Nucleus 2012 v.3 no.6 pp. 565-569
ISSN:: 1949-1042
Subject:: 5-methylcytosine; demethylation; epigenetics; genes; mice
Abstract:: ... 5-Methylcytosine (5-mC) is an epigenetic modification associated with gene repression. Recent studies demonstrated that 5-mC can be enzymatically oxidised into 5-hydroxymethylcytosine and further into 5-formylcytosine (5-fC) and 5-carboxylcytsine (5-caC). 5-caC has been found in embryonic stem cells and in mouse pre-implantation embryos but no detectable levels of this modification have been repor ...
DOI:: 10.4161/nucl.22799
PubMed:: 23138778
PubMed Central:: PMC3515539
: https://doi.org/10.4161/nucl.22799

59. 5-Hydroxymethylcytosine – the elusive epigenetic mark in mammalian DNA

Author:: Edita Kriukienė; Zita Liutkevičiūtė; Saulius Klimašauskas
Source:: Chemical Society reviews 2012 v.41 no.21 pp. 6916-6930
ISSN:: 1460-4744
Subject:: DNA; DNA demethylation; DNA methylation; brain; cell differentiation; embryogenesis; embryonic stem cells; epigenetics; genome; human diseases; humans; iron; mice; neurons; nucleobases; oxygenases
Abstract:: ... Over the past decade, epigenetic phenomena claimed a central role in cell regulatory processes and proved to be important factors for understanding complex human diseases. One of the best understood epigenetic mechanisms is DNA methylation. In the mammalian genome, cytosines (C) were long known to exist in two functional states: unmethylated or methylated at the 5-position of the pyrimidine ring ( ...
DOI:: 10.1039/c2cs35104h
PubMed:: 22842880
PubMed Central:: PMC3467341
: https://doi.org/10.1039/c2cs35104h

60. 500,000 fish phenotypes: The new informatics landscape for evolutionary and developmental biology of the vertebrate skeleton

Author:: P. Mabee; J. P. Balhoff; W. M. Dahdul; H. Lapp; P. E. Midford; T. J. Vision; M. Westerfield
Source:: Journal of applied ichthyology 2012 v.28 no.3 pp. 300-305
ISSN:: 0175-8659
Subject:: Danio rerio; evolution; fish; genes; landscapes; phenotype; skeleton
Abstract:: ... The rich phenotypic diversity that characterizes the vertebrate skeleton results from evolutionary changes in regulation of genes that drive development. Although relatively little is known about the genes that underlie the skeletal variation among fish species, significant knowledge of genetics and development is available for zebrafish. Because developmental processes are highly conserved, this ...
DOI:: 10.1111/j.1439-0426.2012.01985.x
PubMed:: 22736877
PubMed Central:: PMC3377363
: https://doi.org/10.1111/j.1439-0426.2012.01985.x

61. A 54-Kilodalton Protein Encoded by pBtoxis Is Required for Parasporal Body Structural Integrity in Bacillus thuringiensis subsp. israelensis

Author:: Mercedes Diaz-Mendoza; Dennis K. Bideshi; Brian A. Federici
Source:: Journal of bacteriology 2012 v.194 no.6 pp. 1562-1571
ISSN:: 0021-9193
Subject:: Bacillus thuringiensis subsp. israelensis; cytoplasm; endospores; endotoxins; fluorescence microscopy; gene expression; insect larvae; molecular biology; peptides; plasmids; proteins; proteomics; sporulation; toxicity
Abstract:: ... Strains of Bacillus thuringiensis such as B. thuringiensis subsp. israelensis (ONR-60A) and B. thuringiensis subsp. morrisoni (PG-14) pathogenic for mosquito larvae produce a complex parasporal body consisting of several protein endotoxins synthesized during sporulation that form an aggregate of crystalline inclusions bound together by a multilamellar fibrous matrix. Most studies of these strains ...
DOI:: 10.1128/JB.06095-11
PubMed:: 22210770
PubMed Central:: PMC3294862
: https://doi.org/10.1128/JB.06095-11

62. The 5′ untranslated region of the soybean cytosolic glutamine synthetase β₁ gene contains prokaryotic translation initiation signals and acts as a translational enhancer in plants

Author:: Jose Luis Ortega; Olivia L. Wilson; Champa Sengupta-Gopalan
Source:: Molecular genetics and genomics 2012 v.287 no.11-12 pp. 881-893
ISSN:: 1617-4615
Subject:: Escherichia coli; ammonia; bacteria; glutamate-ammonia ligase; glutamine; leaves; reporter genes; soybeans; tobacco; transgenes; translation (genetics)
Abstract:: ... Glutamine synthetase (GS) catalyzes the synthesis of glutamine from glutamate and ammonia. In plants, it occurs as two major isoforms, a cytosolic form (GS₁) and a nuclear encoded chloroplastic form. The focus of this paper is to determine the role of the 5′UTR of a GS₁ gene. GS₁ gene constructs with and without its 5′ and 3′ UTRs, driven by a constitutive promoter, were agroinfiltrated into tobac ...
DOI:: 10.1007/s00438-012-0724-6
PubMed:: 23080263
PubMed Central:: PMC3881598
: https://doi.org/10.1007/s00438-012-0724-6

63. 5′-Terminal chemical capping of spliced leader RNAs

Author:: Karolina Piecyk; Richard E. Davis; Marzena Jankowska-Anyszka
Source:: Tetrahedron letters 2012 v.53 no.36 pp. 4843-4847
ISSN:: 0040-4039
Subject:: Nematoda; biotin; chemical reactions; chemical structure; messenger RNA; mutants; nucleotides; organic compounds; trans-splicing; translation (genetics)
Abstract:: ... Spliced leader (SL) RNA trans-splicing adds a N²,N²,7-trimethylguanosine cap (TMG) and a 22-nucleotide sequence, the SL, to the 5′ end of mRNAs. Both non-trans-spliced with a monomethylguanosine cap (MMG) and trans-spliced mRNAs co-exist in trans-splicing metazoan cells. Efficient translation of TMG-capped mRNAs in nematodes requires a defined core of nucleotides within the SL sequence. Here we pr ...
DOI:: 10.1016/j.tetlet.2012.06.127
PubMed:: 23175583
PubMed Central:: PMC3501006
: https://doi.org/10.1016/j.tetlet.2012.06.127

64. 5′UTR-intron of the Gladiolus polyubiquitin promoter GUBQ1 enhances translation efficiency in Gladiolus and Arabidopsis

Author:: Kathryn Kamo; Ah-Young Kim; Se Hee Park; Young Hee Joung
Source:: BMC Plant Biology 2012 v.12 no. pp. 79-88
ISSN:: 1471-2229
Subject:: 5' untranslated regions; Arabidopsis thaliana; Gladiolus; Southern blotting; beta-glucuronidase; gene expression; genetic transformation; introns; leaves; promoter regions; reporter genes; roots; transgenic plants; translation (genetics); ubiquitin
Abstract:: ... There are many non-cereal monocots of agronomic, horticultural, and biofuel importance. Successful transformation of these species requires an understanding of factors controlling expression of their genes. Introns have been known to affect both the level and tissue-specific expression of genes in dicots and cereal monocots, but there have been no studies on an intron isolated from a non-cereal mo ...
Handle:: 10113/56980
DOI:: 10.1186/1471-2229-12-79
PubMed:: 22672685
PubMed Central:: PMC3406973
: https://doi.org/10.1186/1471-2229-12-79

65. 6-Gingerdiols as the Major Metabolites of 6-Gingerol in Cancer Cells and in Mice and Their Cytotoxic Effects on Human Cancer Cells

Author:: Lv Lishuang; Chen Huadong; Soroka Dominique; Chen Xiaoxin; Leung TinChung; Sang Shengmin
Source:: Journal of agricultural and food chemistry 2012 v.60 no.45 pp. 11372-11377
ISSN:: 1520-5118
Subject:: Zingiber officinale; anticarcinogenic activity; colorectal neoplasms; cytotoxicity; humans; lung neoplasms; metabolites; mice; nuclear magnetic resonance spectroscopy
Abstract:: ... 6-Gingerol, a major pungent component of ginger (Zingiber officinale Roscoe, Zingiberaceae), has been reported to have antitumor activities. However, the metabolic fate of 6-gingerol and the contribution of its metabolites to the observed activities are still unclear. In the present study, we investigated the biotransformation of 6-gingerol in different cancer cells and in mice, purified and ident ...
DOI:: 10.1021/jf303879b
PubMed:: 23066935
PubMed Central:: PMC3649839
: https://doi.org/10.1021/jf303879b

66. The 60-Kilodalton Protein Encoded by orf2 in the cry19A Operon of Bacillus thuringiensis subsp. jegathesan Functions Like a C-Terminal Crystallization Domain

Author:: J. Eleazar Barboza-Corona; Hyun-Woo Park; Dennis K. Bideshi; Brian A. Federici
Source:: Applied and environmental microbiology 2012 v.78 no.6 pp. 2005-2012
ISSN:: 0099-2240
Subject:: Bacillus thuringiensis subsp. jegathesan; Culex quinquefasciatus; amino acid sequences; bioassays; crystallization; crystals; larvae; operon; proteins; sequence analysis; toxicity
Abstract:: ... The cry19A operon of Bacillus thuringiensis subsp. jegathesan encodes two proteins, mosquitocidal Cry19A (ORF1; 75 kDa) and an ORF2 (60 kDa) of unknown function. Expression of the cry19A operon in an acrystalliferous strain of B. thuringiensis (4Q7) yielded one small crystal per cell, whereas no crystals were produced when cry19A or orf2 was expressed alone. To determine the function of the ORF2 p ...
DOI:: 10.1128/AEM.06750-11
PubMed:: 22247140
PubMed Central:: PMC3298145
: https://doi.org/10.1128/AEM.06750-11

67. 6′-Oxa analogs of S-adenosylhomocysteine

Author:: Weikuan Li; Wei Ye; Stewart W. Schneller
Source:: Tetrahedron 2012 v.68 no.1 pp. 65-71
ISSN:: 0040-4020
Subject:: S-adenosylmethionine; chemical structure; methylation
Abstract:: ... S-Adenosylmethionine (AdoMet) is a ubiquitous cofactor in biomethylations and, in that role, becomes S-adenosylhomocysteine (AdoHcy), which serves as a biofeedback inhibitor of the methylation process. In seeking to avail unexplored structural variations of AdoHcy for biological studies, its 6′-oxa analog and two corresponding carbocyclic nucleosides (based on aristeromycin and neplanocin) have be ...
DOI:: 10.1016/j.tet.2011.10.063
PubMed:: 22489098
PubMed Central:: PMC3320718
: https://doi.org/10.1016/j.tet.2011.10.063

68. IL- 7 receptor blockade reverses autoimmune diabetes by promoting inhibition of effector/memory T cells

Author:: Cristina Penaranda; Wilson Kuswanto; Jerry Hofmann; Rupert Kenefeck; Parth Narendran; Lucy S. K. Walker; Jeffrey A. Bluestone; Abul K. Abbas; Hans Dooms
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.31 pp. 12668-12673
ISSN:: 0027-8424
Subject:: T-lymphocytes; animal disease models; autoimmune diseases; autoimmunity; death; diabetes mellitus; interferon-gamma; interleukin-7; mice; monoclonal antibodies; pathogenesis; therapeutics
Abstract:: ... To protect the organism against autoimmunity, self-reactive effector/memory T cells (T E/M) are controlled by cell-intrinsic and -extrinsic regulatory mechanisms. However, how some T E/M cells escape regulation and cause autoimmune disease is currently not understood. Here we show that blocking IL-7 receptor-α (IL-7Rα) with monoclonal antibodies in nonobese diabetic (NOD) mice prevented autoimmune ...
DOI:: 10.1073/pnas.1203692109
PubMed:: 22733744
PubMed Central:: PMC3411948
: https://doi.org/10.1073/pnas.1203692109

69. 7,8,3′-Trihydroxyflavone, a potent small molecule TrkB receptor agonist, protects spiral ganglion neurons from degeneration both in vitro and in vivo

Author:: Qing Yu; Qing Chang; Xia Liu; Shusheng Gong; Keqiang Ye; Xi Lin
Source:: Biochemical and biophysical research communications 2012 v.422 no.3 pp. 387-392
ISSN:: 0006-291X
Subject:: agonists; ears; ganglia; hearing disorders; mice; neurons; receptor protein-tyrosine kinase
Abstract:: ... Most sensorineural hearing loss cases occur as a result of hair cell loss, which results in secondary degeneration of spiral ganglion neurons (SGNs). Substantial loss of SGNs reduces the benefit of cochlear implants, which rely on SGNs for transmitting signals to the central auditory centers. Brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) play essential roles in cochlear develo ...
DOI:: 10.1016/j.bbrc.2012.04.154
PubMed:: 22575512
PubMed Central:: PMC3388121
: https://doi.org/10.1016/j.bbrc.2012.04.154

70. 7-Dehydrocholesterol-derived oxysterols and retinal degeneration in a rat model of Smith–Lemli–Opitz syndrome

Author:: Libin Xu; Lowell G. Sheflin; Ned A. Porter; Steven J. Fliesler
Source:: Biochimica et biophysica acta 2012 v.1821 no.6 pp. 877-883
ISSN:: 1388-1981
Subject:: 7-dehydrocholesterol; animal models; cholesterol; high performance liquid chromatography; histology; intravitreal injection; macular degeneration; mass spectrometry; mice; oxidation; patients; peroxidation; rats; retina; tissues
Abstract:: ... Smith–Lemli–Opitz syndrome (SLOS) is a recessive disease characterized by markedly elevated levels of 7-dehydrocholesterol (7-DHC) and reduced levels of cholesterol in tissues and fluids of affected individuals, due to defective 3β-hydroxysterol-Δ⁷-reductase (Dhcr7). Treatment of Sprague Dawley rats with AY9944 (an inhibitor of Dhcr7) leads to similar biochemical features as observed in SLOS. Eigh ...
DOI:: 10.1016/j.bbalip.2012.03.001
PubMed:: 22425966
PubMed Central:: PMC3340457
: https://doi.org/10.1016/j.bbalip.2012.03.001

71. 7-Methylguanosine Diphosphate (m7GDP) Is Not Hydrolyzed but Strongly Bound by Decapping Scavenger (DcpS) Enzymes and Potently Inhibits Their Activity

Author:: Wypijewska Anna; Bojarska Elzbieta; Lukaszewicz Maciej; Stepinski Janusz; Jemielity Jacek; Davis Richard E.; Darzynkiewicz Edward
Source:: Biochemistry 2012 v.51 no.40 pp. 8003-8013
ISSN:: 1520-4995
Subject:: Nematoda; binding capacity; biochemical pathways; enzyme inhibition; enzymes; humans; hydrolysis; messenger RNA; nucleosides; proteins; yeasts
Abstract:: ... Decapping scavenger (DcpS) enzymes catalyze the cleavage of a residual cap structure following 3′ → 5′ mRNA decay. Some previous studies suggested that both m⁷GpppG and m⁷GDP were substrates for DcpS hydrolysis. Herein, we show that mononucleoside diphosphates, m⁷GDP (7-methylguanosine diphosphate) and m₃²,²,⁷GDP (2,2,7-trimethylguanosine diphosphate), resulting from mRNA decapping by the Dcp1/2 c ...
DOI:: 10.1021/bi300781g
PubMed:: 22985415
PubMed Central:: PMC3495079
: https://doi.org/10.1021/bi300781g

72. The 70S ribosome modulates the ATPase activity of Escherichia coli YchF

Author:: Marion Becker; Katherine E. Gzyl; Alvin M. Altamirano; Anthony Vuong; Kirstin Urbahn; Hans-Joachim Wieden
Source:: RNA biology 2012 v.9 no.10 pp. 1288-1301
ISSN:: 1555-8584
Subject:: Escherichia coli; RNA; adenosinetriphosphatase; catalytic activity; fluorescence; guanine nucleotides; guanosinetriphosphatase; humans; ribosomes
Abstract:: ... YchF is one of two universally conserved GTPases with unknown cellular function. As a first step toward elucidating YchF’s cellular role, we performed a detailed biochemical characterization of the protein from Escherichia coli. Our data from fluorescence titrations not only confirmed the surprising finding that YchFE.cₒₗᵢ binds adenine nucleotides more efficiently than guanine nucleotides, but al ...
DOI:: 10.4161/rna.22131
PubMed:: 22995830
PubMed Central:: PMC3583859
: https://doi.org/10.4161/rna.22131

73. 9,400 years of cosmic radiation and solar activity from ice cores and tree rings

Author:: Friedhelm Steinhilber; Jose A. Abreu; Jürg Beer; Irene Brunner; Marcus Christl; Hubertus Fischer; Ulla Heikkilä; Peter W. Kubik; Mathias Mann; Ken G. McCracken; Heinrich Miller; Hiroko Miyahara; Hans Oerter; Frank Wilhelms
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.16 pp. 5967-5971
ISSN:: 0027-8424
Subject:: climate; greenhouse gases; growth rings; ice; principal component analysis; radionuclides; solar radiation; temporal variation; volcanoes; Antarctica; Greenland
Abstract:: ... Understanding the temporal variation of cosmic radiation and solar activity during the Holocene is essential for studies of the solar-terrestrial relationship. Cosmic-ray produced radionuclides, such as 10Be and 14C which are stored in polar ice cores and tree rings, offer the unique opportunity to reconstruct the history of cosmic radiation and solar activity over many millennia. Although records ...
DOI:: 10.1073/pnas.1118965109
PubMed:: 22474348
PubMed Central:: PMC3341045
: https://doi.org/10.1073/pnas.1118965109

74. 9-Mercaptodethiobiotin Is Generated as a Ligand to the [2Fe–2S]+ Cluster during the Reaction Catalyzed by Biotin Synthase from Escherichia coli

Author:: Fugate Corey J.; Stich Troy A.; Kim Esther G.; Myers William K.; Britt R. David; Jarrett Joseph T.
Source:: Journal of the American Chemical Society 2012 v.134 no.22 pp. 9042-9045
ISSN:: 1520-5126
Subject:: Escherichia coli; S-adenosylmethionine; biotin synthase; electron paramagnetic resonance spectroscopy; free radicals; hydrogen; isotope labeling; ligands; models; sulfur
Abstract:: ... Biotin synthase catalyzes formation of the thiophane ring through stepwise substitution of a sulfur atom for hydrogen atoms at the C9 and C6 positions of dethiobiotin. Biotin synthase is a radical S-adenosylmethionine (SAM) enzyme that reductively cleaves S-adenosylmethionine, generating 5′-deoxyadenosyl radicals that initially abstract a hydrogen atom from the C9 position of dethiobiotin. We have ...
DOI:: 10.1021/ja3012963
PubMed:: 22607542
PubMed Central:: PMC3418058
: https://doi.org/10.1021/ja3012963

75. A 90 day chronic toxicity study of Nigerian herbal preparation DAS-77 in rats

Author:: Saheed O Afolabi; Abidemi J Akindele; Olufunsho Awodele; Chidozie C Anunobi; Olufunmilayo O Adeyemi
Source:: BMC complementary and alternative medicine 2012 v.12 no.1 pp. 79
ISSN:: 1472-6882
Subject:: Carica papaya; Mangifera indica; abnormal development; acute toxicity; adverse effects; alternative medicine; antioxidant activity; antioxidants; bark; blood sampling; body weight; chronic toxicity; electrolytes; enzymes; food intake; herbal medicines; high density lipoprotein; histopathology; kidneys; lethal dose 50; liver; male fertility; males; mice; mortality; neutrophils; potassium; rats; roots; sperm motility; spermatozoa; testes; toxicity testing; Nigeria
Abstract:: ... BACKGROUND: The herbal preparation DAS-77, used for the treatment of various ailments in Nigeria, contains the milled bark of Mangifera indica L. and root of Carica papaya L. Toxicological assessment of the preparation was carried out in this study. METHODS: In the acute toxicity study, DAS-77 was administered to mice p.o. up to 20 g/kg in divided doses and i.p. at 250–3000 mg/kg. Mortality within ...
DOI:: 10.1186/1472-6882-12-79
PubMed:: 22892317
PubMed Central:: PMC3522554
: https://doi.org/10.1186/1472-6882-12-79

76. An A/ENTH Domain-Containing Protein Functions as an Adaptor for Clathrin-Coated Vesicles on the Growing Cell Plate in Arabidopsis Root Cells

Author:: Kyungyoung Song; Mihue Jang; Soo Youn Kim; Goeun Lee; Gil-Je Lee; Dae Heon Kim; Yongjik Lee; Wonhwa Cho; Inhwan Hwang
Source:: Plant physiology 2012 v.159 no.3 pp. 1013-1025
ISSN:: 0032-0889
Subject:: Arabidopsis thaliana; cell walls; clathrin; cytokinesis; endosomes; mitosis; plasma membrane
Abstract:: ... Cytokinesis is the process of partitioning the cytoplasm of a dividing cell, thereby completing mitosis. Cytokinesis in the plant cell is achieved by the formation of a new cell wall between daughter nuclei using components carried in Golgi-derived vesicles that accumulate at the midplane of the phragmoplast and fuse to form the cell plate. Proteins that play major roles in the development of the ...
DOI:: 10.1104/pp.112.199380
PubMed:: 22635117
PubMed Central:: PMC3387690
: https://doi.org/10.1104/pp.112.199380

77. AAA ATPase p97/VCP is essential for TRIM21-mediated virus neutralization

Author:: Felix Hauler; Donna L. Mallery; William A. McEwan; Susanna R. Bidgood; Leo C. James
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.48 pp. 19733-19738
ISSN:: 0027-8424
Subject:: adenosinetriphosphatase; antibodies; capsid; immunity; immunoglobulin G; neutralization; neutralization tests; proteasome endopeptidase complex; virion; viruses
Abstract:: ... Tripartite motif-containing 21 (TRIM21) is a cytosolic IgG receptor that mediates intracellular virus neutralization by antibody. TRIM21 targets virions for destruction in the proteasome, but it is unclear how a substrate as large as a viral capsid is degraded. Here, we identify the ATPase p97/valosin-containing protein (VCP), an enzyme with segregase and unfoldase activity, as a key player in thi ...
DOI:: 10.1073/pnas.1210659109
PubMed:: 23091005
PubMed Central:: PMC3511720
: https://doi.org/10.1073/pnas.1210659109

78. ABA signal in rice under stress conditions

Author:: Nenghui Ye; Liguo Jia; Jianhua Zhang
Source:: Rice 2012 v.5 no.1 pp. 1
ISSN:: 1939-8425
Subject:: abscisic acid; biosynthesis; plant development; plant pathogens; receptors; rice; stress response; transporters
Abstract:: ... Ever since its discovery, abscisic acid (ABA) has been intensively studied due to its versatile functions in plant developmental and physiological processes. Many signaling details of ABA have been well elucidated and reviewed. The identification of ABA receptors is a great breakthrough in the field of ABA study, whereas the discovery of ABA transporter has changed our concept that ABA is delivere ...
DOI:: 10.1186/1939-8433-5-1
PubMed:: 24764501
PubMed Central:: PMC3834477
: https://doi.org/10.1186/1939-8433-5-1

79. The ABA-INSENSITIVE-4 (ABI4) transcription factor links redox, hormone and sugar signaling pathways

Author:: Christine H. Foyer; Pavel I Kerchev; Robert D. Hancock
Source:: Plant signaling & behavior 2012 v.7 no.2 pp. 276-281
ISSN:: 1559-2324
Subject:: abscisic acid; buffering capacity; jasmonic acid; metabolism; plant growth; salicylic acid; signal transduction; sugars; transcription factors
Abstract:: ... The cellular reduction-oxidation (redox) hub processes information from metabolism and the environment and so regulates plant growth and defense through integration with the hormone signaling network. One key pathway of redox control involves interactions with ABSCISIC ACID (ABA). Accumulating evidence suggests that the ABA-INSENSITIVE-4 (ABI4) transcription factor plays a key role in transmitting ...
DOI:: 10.4161/psb.18770
PubMed:: 22415048
PubMed Central:: PMC3404864
: https://doi.org/10.4161/psb.18770

80. ABC1K atypical kinases in plants: filling the organellar kinase void

Author:: Peter K. Lundquist; Jerrold I. Davis; Klaas J. van Wijk
Source:: Trends in plant science 2012 v.17 no.9 pp. 546-555
ISSN:: 1360-1385
Subject:: Arabidopsis thaliana; Zea mays; metabolism; protein kinases; rice; Oryza sativa; corn; gene expression; mitochondria
Abstract:: ... Surprisingly few protein kinases have been demonstrated in chloroplasts or mitochondria. Here, we discuss the activity of bc₁ complex kinase (ABC1K) protein family, which we suggest locate in mitochondria and plastids, thus filling the kinase void. The ABC1Ks are atypical protein kinases and their ancestral function is the regulation of quinone synthesis. ABC1Ks have proliferated from one or two m ...
DOI:: 10.1016/j.tplants.2012.05.010
PubMed:: 22694836
PubMed Central:: PMC3926664
: https://doi.org/10.1016/j.tplants.2012.05.010

81. The ABCs of molecular dynamics simulations on B-DNA, circa 2012

Author:: David L Beveridge; Thomas E Cheatham III; Mihaly Mezei
Source:: Journal of biosciences 2012 v.37 no.3 pp. 379-397
ISSN:: 0250-5991
Subject:: B-DNA; databases; genes; ions; models; molecular dynamics; nuclear magnetic resonance spectroscopy; nucleotide sequences
Abstract:: ... This article provides a retrospective on the ABC initiative in the area of all-atom molecular dynamics (MD) simulations including explicit solvent on all tetranucleotide steps of duplex B-form DNA duplex, ca. 2012. The ABC consortium has completed two phases of simulations, the most current being a set of 50â100 trajectories based on the AMBER ff99 force field together with the parmbsc0 modifica ...
DOI:: 10.1007/s12038-012-9222-6
PubMed:: 22750978
PubMed Central:: PMC4029509
: https://doi.org/10.1007/s12038-012-9222-6

82. The ABO blood group is a trans-species polymorphism in primates

Author:: Laure Ségurel; Emma E. Thompson; Timothée Flutre; Jessica Lovstad; Aarti Venkat; Susan W. Margulis; Jill Moyse; Steve Ross; Kathryn Gamble; Guy Sella; Carole Ober; Molly Przeworski
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.45 pp. 18493-18498
ISSN:: 0027-8424
Subject:: Cercopithecidae; Hominidae; Hylobatidae; alleles; amino acids; antigens; blood groups; convergent evolution; genetic polymorphism; genetic variation; humans; major histocompatibility complex; models
Abstract:: ... The ABO histo-blood group, the critical determinant of transfusion incompatibility, was the first genetic polymorphism discovered in humans. Remarkably, ABO antigens are also polymorphic in many other primates, with the same two amino acid changes responsible for A and B specificity in all species sequenced to date. Whether this recurrence of A and B antigens is the result of an ancient polymorphi ...
DOI:: 10.1073/pnas.1210603109
PubMed:: 23091028
PubMed Central:: PMC3494955
: https://doi.org/10.1073/pnas.1210603109

83. ACCELERATED CELL DEATH 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis

Author:: Gopal K. Pattanayak; Sujatha Venkataramani; Stefan Hortensteiner; Lukas Kunz; Bastien Christ; Michael Moulin; Alison G. Smith; Yukihiro Okamoto; Hitoshi Tamiaki; Masakazu Sugishima; Jean T. Greenberg
Source:: plant journal 2012 v.69 no.4 pp. 589-600
ISSN:: 0960-7412
Subject:: Arabidopsis; apoptosis; bacterial infections; chlorophyll; chloroplasts; enzymes; leaves; mitochondria; oxygen; phenotype; proteins; protoporphyrin
Abstract:: ... The Arabidopsis ACCELERATED CELL DEATH 2 (ACD2) protein protects cells from programmed cell death (PCD) caused by endogenous porphyrin‐related molecules like red chlorophyll catabolite or exogenous protoporphyrin IX. We previously found that during bacterial infection, ACD2, a chlorophyll breakdown enzyme, localizes to both chloroplasts and mitochondria in leaves. Additionally, acd2 cells show mit ...
DOI:: 10.1111/j.1365-313X.2011.04814.x
PubMed:: 21988537
PubMed Central:: PMC3274588
: https://doi.org/10.1111/j.1365-313X.2011.04814.x

84. ADAM13 function is required in the 3 dimensional context of the embryo during cranial neural crest cell migration in Xenopus laevis

Author:: Hélène Cousin; Genevieve Abbruzzese; Catherine McCusker; Dominique Alfandari
Source:: Developmental biology 2012 v.368 no.2 pp. 335-344
ISSN:: 0012-1606
Subject:: Xenopus laevis; brain; cell movement; genes; metalloproteinases; neural crest; proteins; tissues; vertebrates
Abstract:: ... The cranial neural crest (CNC) is a population of cells that arises from the lateral part of the developing brain, migrates ventrally and coordinates the entire craniofacial development of vertebrates. Many molecules are involved in CNC migration including the transmembrane metalloproteases ADAM13 and 19. We have previously shown that these ADAMs cleave a number of extracellular proteins and modif ...
DOI:: 10.1016/j.ydbio.2012.05.036
PubMed:: 22683825
PubMed Central:: PMC3402629
: https://doi.org/10.1016/j.ydbio.2012.05.036

85. The ADAR protein family

Author:: Yiannis A Savva; Leila E Rieder; Robert A Reenan
Source:: Genome biology 2012 v.13 no.12 pp. 2975
ISSN:: 1474-760X
Subject:: RNA editing; RNA interference; adenosine; adenosine deaminase; double-stranded RNA; gene expression; genes; ion channels; mutation; nervous system; peptides; phenotype
Abstract:: ... Adenosine to inosine (A-to-I) RNA editing is a post-transcriptional process by which adenosines are selectively converted to inosines in double-stranded RNA (dsRNA) substrates. A highly conserved group of enzymes, the adenosine deaminase acting on RNA (ADAR) family, mediates this reaction. All ADARs share a common domain architecture consisting of a variable number of amino-terminal dsRNA binding ...
DOI:: 10.1186/gb-2012-13-12-252
PubMed:: 23273215
PubMed Central:: PMC3580408
: https://doi.org/10.1186/gb-2012-13-12-252

86. The AGC kinase, PINOID, blocks interactive ABCB/PIN auxin transport

Author:: Bangjun Wang; Sina Henrichs; Markus Geisler
Source:: Plant signaling & behavior 2012 v.7 no.12 pp. 1515-1517
ISSN:: 1559-2324
Subject:: auxins; catalysts; enzyme activity; growth and development; plant development; plant growth; protein kinases; protein phosphorylation; yeasts
Abstract:: ... Plant growth and development is determined by intracellular and intercellular auxin gradients that are controlled at first hand by auxin efflux catalysts of the ABCB/PGP and PIN families. ABCB transport activity was shown to be counter-actively regulated by protein phosphorylation by the AGC protein kinase, PINOID (PID), that is coordinated by interaction with the immunophilin-like FKBP42, TWISTED ...
DOI:: 10.4161/psb.22093
PubMed:: 23073023
PubMed Central:: PMC3578881
: https://doi.org/10.4161/psb.22093

87. AGL24 acts in concert with SOC1 and FUL during Arabidopsis floral transition

Author:: Stefano Torti; Fabio Fornara
Source:: Plant signaling & behavior 2012 v.7 no.10 pp. 1251-1254
ISSN:: 1559-2324
Subject:: Arabidopsis; apical meristems; flowering; flowers; leaves; messenger RNA; mutants; mutation
Abstract:: ... Arabidopsis plants flower in response to long days (LDs). Exposure of leaves to inductive day lengths activates expression of FLOWERING LOCUS T (FT) protein which moves to the shoot apical meristem (SAM) to induce developmental reprogramming. SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) and FRUITFULL (FUL) are induced by FT at the apex. We previously screened the SAM for mRNAs of genes requir ...
DOI:: 10.4161/psb.21552
PubMed:: 22902690
PubMed Central:: PMC3493406
: https://doi.org/10.4161/psb.21552

88. AGNPS GIS-Based Tool for Watershed-Scale Identification and Mapping of Cropland Potential Ephemeral Gullies

Author:: Momm, H.G.; Bingner, R.L.; Wells, R.R.; Wilcox, D.
Source:: Applied engineering in agriculture 2012 v.28 no.1 pp. 17-29
ISSN:: 1943-7838
Subject:: AGNPS model; agricultural land; case studies; conservation practices; geographic information systems; gully erosion; pollution; sediment yield; sediments; user interface; watersheds
Abstract:: ... AnnAGNPS, Compound topographic index, Ephemeral gully, GIS, Cropland erosion. The formation of ephemeral gullies in agricultural fields has been recognized as an important source of sediment contributing to environmental degradation and compromising crop productivity. Methodologies are being developed for assessing gully formation and gully sediment yield. The Annualized Agricultural Non-Point Sou ...
Handle:: 10113/54162
DOI:: 10.13031/2013.41282
: https://doi.org/10.13031/2013.41282

89. The AINTEGUMENTA LIKE1 Homeotic Transcription Factor PtAIL1 Controls the Formation of Adventitious Root Primordia in Poplar

Author:: Adeline Rigal; Yordan S. Yordanov; Irene Perrone; Anna Karlberg; Emilie Tisserant; Catherine Bellini; Victor B. Busov; Francis Martin; Annegret Kohler; Rishi Bhalerao; Valérie Legué
Source:: Plant physiology 2012 v.160 no.4 pp. 1996-2006
ISSN:: 0032-0889
Subject:: germplasm; transcription factors; genetically modified organisms; RNA interference; gene overexpression; microarray technology; early development; adventitious roots; Populus trichocarpa; mature plants; rooting; genes; root primordia; transcription (genetics); transcriptome; elite trees
Abstract:: ... Adventitious rooting is an essential but sometimes rate-limiting step in the clonal multiplication of elite tree germplasm, because the ability to form roots declines rapidly with age in mature adult plant tissues. In spite of the importance of adventitious rooting, the mechanism behind this developmental process remains poorly understood. We have described the transcriptional profiles that are as ...
DOI:: 10.1104/pp.112.204453
PubMed:: 23077242
PubMed Central:: PMC3510126
: https://doi.org/10.1104/pp.112.204453

90. ALLENE OXIDE CYCLASE (AOC) gene family members of Arabidopsis thaliana: tissue- and organ-specific promoter activities and in vivo heteromerization*

Author:: Irene Stenzel; Markus Otto; Carolin Delker; Nils Kirmse; Diana Schmidt; Otto Miersch; Bettina Hause; Claus Wasternack
Source:: Journal of experimental botany 2012 v.63 no.17 pp. 6125-6138
ISSN:: 0022-0957
Subject:: Arabidopsis thaliana; biosynthesis; flowering; flowers; fluorescence; gene expression regulation; genetically modified organisms; in situ hybridization; jasmonic acid; leaves; male fertility; mutants; phenotype; plant response; plant stress; polypeptides; reporter genes; roots; stress response
Abstract:: ... Jasmonates are important signals in plant stress responses and plant development. An essential step in the biosynthesis of jasmonic acid (JA) is catalysed by ALLENE OXIDE CYCLASE (AOC) which establishes the naturally occurring enantiomeric structure of jasmonates. In Arabidopsis thaliana, four genes encode four functional AOC polypeptides (AOC1, AOC2, AOC3, and AOC4) raising the question of functi ...
DOI:: 10.1093/jxb/ers261
PubMed:: 23028017
PubMed Central:: PMC3481204
: https://doi.org/10.1093/jxb/ers261

91. AMDD: Antimicrobial Drug Database

Author:: Mohd Danishuddin; Lalima Kaushal; Mohd Hassan Baig; Asad U. Khan
Source:: Genomics, proteomics & bioinformatics 2012 v.10 no.6 pp. 360-363
ISSN:: 1672-0229
Subject:: screening; drug therapy; antibiotics; antifungal agents; bioassays; hydrogen bonding; Internet; molecular weight; databases; drug resistance
Abstract:: ... Drug resistance is one of the major concerns for antimicrobial chemotherapy against any particular target. Knowledge of the primary structure of antimicrobial agents and their activities is essential for rational drug design. Thus, we developed a comprehensive database, anti microbial drug database (AMDD), of known synthetic antibacterial and antifungal compounds that were extracted from the avail ...
DOI:: 10.1016/j.gpb.2012.04.002
PubMed:: 23317704
PubMed Central:: PMC5054706
: https://doi.org/10.1016/j.gpb.2012.04.002

92. AMP-Forming Acetyl Coenzyme A Synthetase in the Outermost Membrane of the Hyperthermophilic Crenarchaeon Ignicoccus hospitalis

Author:: Florian Mayer; Ulf KÃ¼per; Carolin Meyer; Stefanie Daxer; Volker MÃ¼ller; Reinhard Rachel; Harald Huber
Source:: Journal of bacteriology 2012 v.194 no.6 pp. 1572-1581
ISSN:: 0021-9193
Subject:: H-transporting ATP synthase; acetate-CoA ligase; acetates; acetyl coenzyme A; adenosine triphosphate; bioinformatics; carbon dioxide; desorption; molecular weight; tandem mass spectrometry
Abstract:: ... Ignicoccus hospitalis, a hyperthermophilic, chemolithoautotrophic crenarchaeon was found to possess a new CO2 fixation pathway, the dicarboxylate/4-hydroxybutyrate cycle. The primary acceptor molecule for this pathway is acetyl coenzyme A (acetyl-CoA), which is regenerated in the cycle via the characteristic intermediate 4-hydroxybutyrate. In the presence of acetate, acetyl-CoA can alternatively b ...
DOI:: 10.1128/JB.06130-11
PubMed:: 22247508
PubMed Central:: PMC3294865
: https://doi.org/10.1128/JB.06130-11

93. AMP-activated protein kinase is physiologically regulated by inositol polyphosphate multikinase

Author:: Sookhee Bang; Seyun Kim; Megan J. Dailey; Yong Chen; Timothy H. Moran; Solomon H. Snyder; Sangwon F. Kim
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.2 pp. 616-620
ISSN:: 0027-8424
Subject:: AMP-activated protein kinase; diabetes; energy; food intake; gene expression regulation; glucose; mice; myo-inositol; obesity; phosphorylation; refeeding; transcription (genetics); tyrosine
Abstract:: ... The AMP-activated kinase (AMPK) senses the energy status of cells and regulates fuel availability, whereas hypothalamic AMPK regulates food intake. We report that inositol polyphosphate multikinase (IPMK) regulates glucose signaling to AMPK in a pathway whereby glucose activates phosphorylation of IPMK at tyrosine 174 enabling the enzyme to bind to AMPK and regulate its activation. Thus, refeeding ...
DOI:: 10.1073/pnas.1119751109
PubMed:: 22203993
PubMed Central:: PMC3258619
: https://doi.org/10.1073/pnas.1119751109

94. AMP-activated protein kinase stimulates myostatin expression in C2C12 cells

Author:: Arun K. Das; Qi-Yuan Yang; Xing Fu; Jun-Fang Liang; Marcio S. Duarte; Mei-Jun Zhu; Grant D. Trobridge; Min Du
Source:: Biochemical and biophysical research communications 2012 v.427 no.1 pp. 36-40
ISSN:: 0006-291X
Subject:: AMP-activated protein kinase; energy metabolism; gene expression; mammals; messenger RNA; metformin; models; muscle protein; muscles; mutants; myoblasts; myostatin; protein content; protein degradation; protein synthesis; skeletal muscle
Abstract:: ... AMP-activated protein kinase (AMPK) is a master regulator of energy metabolism in skeletal muscle; AMPK induces muscle protein degradation but the underlying mechanisms are unclear. Myostatin is a powerful negative regulator of skeletal muscle mass and growth in mammalian species. We hypothesized that AMPK stimulates myostatin expression, which provides an explanation for the negative role of AMPK ...
DOI:: 10.1016/j.bbrc.2012.08.138
PubMed:: 22995402
PubMed Central:: PMC3486733
: https://doi.org/10.1016/j.bbrc.2012.08.138

95. ANAP: An Integrated Knowledge Base for Arabidopsis Protein Interaction Network Analysis

Author:: Congmao Wang; Alex Marshall; Dabing Zhang; Zoe A. Wilson
Source:: Plant physiology 2012 v.158 no.4 pp. 1523-1533
ISSN:: 0032-0889
Subject:: Arabidopsis thaliana; bioinformatics; databases; genes; information sources; proteins
Abstract:: ... Protein interactions are fundamental to the molecular processes occurring within an organism and can be utilized in network biology to help organize, simplify, and understand biological complexity. Currently, there are more than 10 publicly available Arabidopsis (Arabidopsis thaliana) protein interaction databases. However, there are limitations with these databases, including different types of i ...
DOI:: 10.1104/pp.111.192203
PubMed:: 22345505
PubMed Central:: PMC3320167
: https://doi.org/10.1104/pp.111.192203

96. The APC/C Subunit Mnd2/Apc15 Promotes Cdc20 Autoubiquitination and Spindle Assembly Checkpoint Inactivation

Author:: Scott A. Foster; David O. Morgan
Source:: Molecular cell 2012 v.47 no.6 pp. 921-932
ISSN:: 1097-2765
Subject:: anaphase; chromosome segregation; kinetochores; ubiquitination; yeasts
Abstract:: ... The fidelity of chromosome segregation depends on the spindle assembly checkpoint (SAC). In the presence of unattached kinetochores, anaphase is delayed when three SAC components (Mad2, Mad3/BubR1, and Bub3) inhibit Cdc20, the activating subunit of the anaphase-promoting complex (APC/C). We analyzed the role of Cdc20 autoubiquitination in the SAC of budding yeast. Reconstitution with purified comp ...
DOI:: 10.1016/j.molcel.2012.07.031
PubMed:: 22940250
PubMed Central:: PMC3462283
: https://doi.org/10.1016/j.molcel.2012.07.031

97. APOA5 genotype modulates 2-y changes in lipid profile in response to weight-loss diet intervention: the Pounds Lost Trial

Author:: Xiaomin Zhang; Qibin Qi; George A Bray; Frank B Hu; Frank M Sacks; Lu Qi
Source:: American journal of clinical nutrition 2012 v.96 no.4 pp. 917-922
ISSN:: 0002-9165
Subject:: adults; alleles; blood serum; clinical nutrition; data collection; energy; fasting; fat intake; genotype; high density lipoprotein cholesterol; high fat diet; lipid metabolism; low density lipoprotein cholesterol; low fat diet; major genes; nutritional intervention; overweight; risk; triacylglycerols; weight loss
Abstract:: ... Background: The apolipoprotein A5 gene (APOA5) is a major gene that regulates lipid metabolism and is modulated by dietary factors. A novel variant rs964184 in APOA5 was identified to be associated with lipids in genome-wide association studies.Objective: We examined whether this variant modified changes in lipid concentrations in response to a 2-y weight-loss diet intervention in a randomized tri ...
DOI:: 10.3945/ajcn.112.040907
PubMed:: 22914552
PubMed Central:: PMC3441115
: https://doi.org/10.3945/ajcn.112.040907

98. APOBEC2 Is a Monomer in Solution: Implications for APOBEC3G Models

Author:: Krzysiak Troy C.; Jung Jinwon; Thompson James; Baker David; Gronenborn Angela M.
Source:: Biochemistry 2012 v.51 no.9 pp. 2008-2017
ISSN:: 1520-4995
Subject:: Human immunodeficiency virus; antiretroviral properties; crystal structure; crystallization; models; nuclear magnetic resonance spectroscopy; pathogenicity; prediction
Abstract:: ... Although the physiological role of APOBEC2 is still largely unknown, a crystal structure of a truncated variant of this protein was determined several years ago [Prochnow, C. (2007) Nature445, 447–451]. This APOBEC2 structure had considerable impact in the HIV field because it was considered a good model for the structure of APOBEC3G, an important HIV restriction factor that abrogates HIV infectiv ...
DOI:: 10.1021/bi300021s
PubMed:: 22339232
PubMed Central:: PMC3316327
: https://doi.org/10.1021/bi300021s

99. APOBEC3B and AID Have Similar Nuclear Import Mechanisms

Author:: Lela Lackey; Zachary L. Demorest; Allison M. Land; Judd F. Hultquist; William L. Brown; Reuben S. Harris
Source:: Journal of molecular biology 2012 v.419 no.5 pp. 301-314
ISSN:: 0022-2836
Subject:: DNA; DNA damage; Primates; amino acids; apolipoprotein B; cell cycle; cytosine; cytosine deaminase; deamination; genes; importins; loci; messenger RNA; physiological transport; retrotransposons
Abstract:: ... Members of the APOBEC (apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like) protein family catalyze DNA cytosine deamination and underpin a variety of immune defenses. For instance, several family members, including APOBEC3B (A3B), elicit strong retrotransposon and retrovirus restriction activities. However, unlike the other proteins, A3B is the only family member with steady-state nu ...
DOI:: 10.1016/j.jmb.2012.03.011
PubMed:: 22446380
PubMed Central:: PMC3368237
: https://doi.org/10.1016/j.jmb.2012.03.011

100. The AR dependent cell cycle: Mechanisms and cancer relevance

Author:: Matthew J. Schiewer; Michael A. Augello; Karen E. Knudsen
Source:: Molecular and cellular endocrinology 2012 v.352 no.1-2 pp. 34-45
ISSN:: 0303-7207
Subject:: androgen receptors; cell cycle; cell proliferation; human diseases; neoplasm cells; prostatic neoplasms
Abstract:: ... Prostate cancer cells are exquisitely dependent on androgen receptor (AR) activity for proliferation and survival. As these functions are critical targets of therapeutic intervention for human disease, it is imperative to delineate the mechanisms by which AR engages the cell cycle engine. More than a decade of research has revealed that elegant intercommunication between AR and the cell cycle mach ...
DOI:: 10.1016/j.mce.2011.06.033
PubMed:: 21782001
PubMed Central:: PMC3641823
: https://doi.org/10.1016/j.mce.2011.06.033

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1. "Evolution Canyon," a potential microscale monitor of global warming across life

2. "tet(U)" Is Not a Tetracycline Resistance Determinant

3. (n-3) Fatty Acids and Cardiovascular Health: Are Effects of EPA and DHA Shared or Complementary?

4. (φ,ψ)₂ Motifs: A Purely Conformation-Based Fine-Grained Enumeration of Protein Parts at the Two-Residue Level

5. (−)-Epicatechin prevents TNFα-induced activation of signaling cascades involved in inflammation and insulin sensitivity in 3T3-L1 adipocytes

6. L: -Theanine extends lifespan of adult Caenorhabditis elegans

7. 1,2,3-Triazole–Heme Interactions in Cytochrome P450: Functionally Competent Triazole–Water–Heme Complexes

8. The 1,2-Benzenedithiole-Based Cyclocondensation Assay: A Valuable Tool for the Measurement of Chemopreventive Isothiocyanates

9. 1,2-dielaidoylphosphocholine/1,2-dimyristoylphosphoglycerol supported phospholipid bilayer formation in calcium and calcium-free buffer

10. 1,4-Diazaspiro[2.2]pentanes as a Flexible Platform for the Synthesis of Diamine-Bearing Stereotriads

11. 1,500 year quantitative reconstruction of winter precipitation in the Pacific Northwest

12. 1-Methylthio-d-xylulose 5-Phosphate Methylsulfurylase: A Novel Route to 1-Deoxy-d-xylulose 5-Phosphate in Rhodospirillum rubrum

13. The 10-Year Cost-Effectiveness of Lifestyle Intervention or Metformin for Diabetes Prevention: An intent-to-treat analysis of the DPP/DPPOS

14. 100% Orange juice consumption is associated with better diet quality, improved nutrient adequacy, decreased risk for obesity, and improved biomarkers of health in adults: National Health and Nutrition Examination Survey, 2003-2006

15. IL- 10–induced microRNA-187 negatively regulates TNF-α, IL-6, and IL-12p40 production in TLR4-stimulated monocytes

16. A 12-week double-blind randomized clinical trial of vitamin D₃ supplementation on body fat mass in healthy overweight and obese women

17. 14-3-3 Proteins SGF14c and SGF14l Play Critical Roles during Soybean Nodulation

18. 14-3-3 fusion oncogenes in high-grade endometrial stromal sarcoma

19. 14-3-3 proteins act as scaffolds for GmMYB62 and GmMYB176 and regulate their intracellular localization in soybean

20. The 14-3-3 proteins of Arabidopsis regulate root growth and chloroplast development as components of the photosensory system

21. 15N-Labeled Brain Enables Quantification of Proteome and Phosphoproteome in Cultured Primary Neurons

22. 15N-Labeled Full-Length Apolipoprotein E4 as an Internal Standard for Mass Spectrometry Quantification of Apolipoprotein E Isoforms

23. 17 novel polymorphic microsatellite markers for the giant water bug, Abedus herberti (Belostomatidae)

24. 1H Dynamic Nuclear Polarization Based on an Endogenous Radical

25. 1α,25-Dihydroxyvitamin D₃ and its analog, 2-methylene-19-nor-(20S)-1α,25-dihydroxyvitamin D₃ (2MD), suppress intraocular pressure in non-human primates

26. 1’-Acetoxychavicol acetate inhibits growth of human oral carcinoma xenograft in mice and potentiates cisplatin effect via proinflammatory microenvironment alterations

27. 2,2,2-Trifluoroethyl 6-thio-β-d-glucopyranoside as a selective tag for cysteines in proteins

28. 2,2′,3,5′,6-Pentachlorobiphenyl (PCB 95) and Its Hydroxylated Metabolites Are Enantiomerically Enriched in Female Mice

29. 2,3-Hexanediols as Sex Attractants and a Female-produced Sex Pheromone for Cerambycid Beetles in the Prionine Genus Tragosoma

30. 2-Hydroxyoleate, a nontoxic membrane binding anticancer drug, induces glioma cell differentiation and autophagy

31. 2-Methoxyestradiol inhibits experimental autoimmune encephalomyelitis through suppression of immune cell activation

32. The 2009 pandemic H1N1 virus induces anti-neuraminidase (NA) antibodies that cross-react with the NA of H5N1 viruses in ferrets

33. A 21-day Daniel fast with or without krill oil supplementation improves anthropometric parameters and the cardiometabolic profile in men and women

34. The 21.5-kDa isoform of myelin basic protein has a non-traditional PY-nuclear-localization signal

35. 24-Hour profiles of circulating ghrelin and peptide YY are inversely associated in normal weight premenopausal women

36. 25-Hydroxyvitamin D Concentrations and In Vivo Insulin Sensitivity and β-Cell Function Relative to Insulin Sensitivity in Black and White Youth

37. 25-Hydroxyvitamin D deficiency is associated with fatal stroke among whites but not blacks: The NHANES-III linked mortality files

38. 26S Proteasome regulation of Ankrd1/CARP in adult rat ventricular myocytes and human microvascular endothelial cells

39. The 27–year decline of coral cover on the Great Barrier Reef and its causes

40. A 29-mer site regulates transcription of the initiator gene as well as function of the replication origin of Vibrio cholerae chromosome II

41. The 2b proteins of Cucumber mosaic virus generally have the potential to differentially induce necrosis on Arabidopsis

42. 3'-UTR-located inverted Alu repeats facilitate mRNA translational repression and stress granule accumulation

43. 3-D and Quasi-2-D Discrete Element Modeling of Grain Commingling in a Bucket Elevator Boot System

44. 3-Dimensional Imaging Modalities for Phenotyping Genetically Engineered Mice

45. 3-Helix Micelles Stabilized by Polymer Springs

46. 3-Picolyl Azide Adenine Dinucleotide as a Probe of Femtosecond to Picosecond Enzyme Dynamics

47. IL- 33 is processed into mature bioactive forms by neutrophil elastase and cathepsin G

48. IL- 38 binds to the IL-36 receptor and has biological effects on immune cells similar to IL-36 receptor antagonist

49. 3D cell entrapment in crosslinked thiolated gelatin-poly(ethylene glycol) diacrylate hydrogels

50. 3D imaging and mechanical modeling of helical buckling in Medicago truncatula plant roots

51. A 3D localized surface plasmon resonance biosensor for the study of trivalent arsenic binding to the ArsA ATPase

52. 3D superhydrophobic electrospun meshes as reinforcement materials for sustained local drug delivery against colorectal cancer cells

53. 3β-Methoxy-pregnenolone (MAP4343) as an innovative therapeutic approach for depressive disorders

54. 3′ Phosphatase activity toward phosphatidylinositol 3,4-bisphosphate [PI(3,4)P₂] by voltage-sensing phosphatase (VSP)

55. 4-Hydroxynonenal differentially regulates adiponectin gene expression and secretion via activating PPARγ and accelerating ubiquitin–proteasome degradation

56. 4-O-methylation of glucuronic acid in Arabidopsis glucuronoxylan is catalyzed by a domain of unknown function family 579 protein

57. 470-Million-year-old black corals from China

58. 5-Carboxylcytosine is localized to euchromatic regions in the nuclei of follicular cells in axolotl ovary

59. 5-Hydroxymethylcytosine – the elusive epigenetic mark in mammalian DNA

60. 500,000 fish phenotypes: The new informatics landscape for evolutionary and developmental biology of the vertebrate skeleton

61. A 54-Kilodalton Protein Encoded by pBtoxis Is Required for Parasporal Body Structural Integrity in Bacillus thuringiensis subsp. israelensis

62. The 5′ untranslated region of the soybean cytosolic glutamine synthetase β₁ gene contains prokaryotic translation initiation signals and acts as a translational enhancer in plants

63. 5′-Terminal chemical capping of spliced leader RNAs

64. 5′UTR-intron of the Gladiolus polyubiquitin promoter GUBQ1 enhances translation efficiency in Gladiolus and Arabidopsis

65. 6-Gingerdiols as the Major Metabolites of 6-Gingerol in Cancer Cells and in Mice and Their Cytotoxic Effects on Human Cancer Cells

66. The 60-Kilodalton Protein Encoded by orf2 in the cry19A Operon of Bacillus thuringiensis subsp. jegathesan Functions Like a C-Terminal Crystallization Domain

67. 6′-Oxa analogs of S-adenosylhomocysteine

68. IL- 7 receptor blockade reverses autoimmune diabetes by promoting inhibition of effector/memory T cells

69. 7,8,3′-Trihydroxyflavone, a potent small molecule TrkB receptor agonist, protects spiral ganglion neurons from degeneration both in vitro and in vivo