Publication Year: 2012 / Text Availability: Full Text

1. "Evolution Canyon," a potential microscale monitor of global warming across life

Author:: Eviatar Nevo
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.8 pp. 2960-2965
ISSN:: 0027-8424
Subject:: Drosophila; bacteria; biodiversity; drought; extinction; fungi; global warming; heat; mammals; microclimate; models; photosynthesis; prediction; shrublands; solar radiation; sympatric speciation; viruses; Israel
Abstract:: ... Climatic change and stress is a major driving force of evolution. The effects of climate change on living organisms have been shown primarily on regional and global scales. Here I propose the "Evolution Canyon" (EC) microscale model as a potential life monitor of global warming in Israel and the rest of the world. The EC model reveals evolution in action at a microscale involving biodiversity dive ...
DOI:: 10.1073/pnas.1120633109
PubMed:: 22308456
PubMed Central:: PMC3286920
: https://doi.org/10.1073/pnas.1120633109

2. "tet(U)" Is Not a Tetracycline Resistance Determinant

Author:: Jamie A. Caryl; Georgina Cox; Stefan Trimble; Alex J. O'Neill
Source:: Antimicrobial agents and chemotherapy 2012 v.56 no.6 pp. 3378-3379
ISSN:: 0066-4804
Subject:: antibiotic resistance; bacterial proteins; bioinformatics; genes; plasmids; resistance mechanisms; tetracycline
Abstract:: ... The enterococcal plasmid pKQ10 has been reported to carry a poorly characterized tetracycline resistance determinant designated tet(U). However, in a series of studies intended to further characterize this determinant, we have been unable to substantiate the claim that tet(U) confers resistance to tetracyclines. In line with these results, bioinformatic analysis provides compelling evidence that " ...
DOI:: 10.1128/AAC.05957-11
PubMed:: 22491689
PubMed Central:: PMC3370814
: https://doi.org/10.1128/AAC.05957-11

3. (n-3) Fatty Acids and Cardiovascular Health: Are Effects of EPA and DHA Shared or Complementary?

Author:: Dariush Mozaffarian; Jason H. Y. Wu
Source:: Journal of nutrition 2012 v.142 no.3 pp. 614S-625S
ISSN:: 0022-3166
Subject:: docosapentaenoic acid; high density lipoprotein; fish consumption; humans; compliance; inflammation; laboratory animals; endogenous sources; particle size; platelet aggregation; low density lipoprotein; risk factors; animal models; arrhythmia; fish oils; clinical trials; oxidative stress; research support
Abstract:: ... Considerable research supports cardiovascular benefits of consuming omega-3 PUFA, also known as (n-3) PUFA, from fish or fish oil. Whether individual long-chain (n-3) PUFA have shared or complementary effects is not well established. We reviewed evidence for dietary and endogenous sources and cardiovascular effects on biologic pathways, physiologic risk factors, and clinical endpoints of EPA [20:5 ...
DOI:: 10.3945/jn.111.149633
PubMed:: 22279134
PubMed Central:: PMC3278271
: https://doi.org/10.3945/jn.111.149633

4. (φ,ψ)₂ Motifs: A Purely Conformation-Based Fine-Grained Enumeration of Protein Parts at the Two-Residue Level

Author:: Scott A. Hollingsworth; Matthew C. Lewis; Donald S. Berkholz; Weng-Keen Wong; P. Andrew Karplus
Source:: Journal of molecular biology 2012 v.416 no.1 pp. 78-93
ISSN:: 0022-2836
Subject:: algorithms; models; polypeptides; population density; protein structure; proteins
Abstract:: ... A deep understanding of protein structure benefits from the use of a variety of classification strategies that enhance our ability to effectively describe local patterns of conformation. Here, we use a clustering algorithm to analyze 76,533 all-trans segments from protein structures solved at 1.2 Å resolution or better to create a purely φ,ψ-based comprehensive empirical categorization of common c ...
DOI:: 10.1016/j.jmb.2011.12.022
PubMed:: 22198294
PubMed Central:: PMC3268948
: https://doi.org/10.1016/j.jmb.2011.12.022

5. 1,2,3-Triazole–Heme Interactions in Cytochrome P450: Functionally Competent Triazole–Water–Heme Complexes

Author:: Conner Kip P.; Vennam Preethi; Woods Caleb M.; Krzyaniak Matthew D.; Bowman Michael K.; Atkins William M.
Source:: Biochemistry 2012 v.51 no.32 pp. 6441-6457
ISSN:: 1520-4995
Subject:: cytochrome P-450; electron paramagnetic resonance spectroscopy; enthalpy; entropy; heme; heme iron; hydrogen bonding; ligands; metabolism; models; risk
Abstract:: ... In comparison to imidazole (IMZ) and 1,2,4-triazole (1,2,4-TRZ), the isosteric 1,2,3-triazole (1,2,3-TRZ) is unrepresented among cytochrome P450 (CYP) inhibitors. This is surprising because 1,2,3-TRZs are easily obtained via “click” chemistry. To understand this underrepresentation of 1,2,3-TRZs among CYP inhibitors, thermodynamic and density functional theory computational studies were performed ...
DOI:: 10.1021/bi300744z
PubMed:: 22809252
PubMed Central:: PMC3448000
: https://doi.org/10.1021/bi300744z

6. The 1,2-Benzenedithiole-Based Cyclocondensation Assay: A Valuable Tool for the Measurement of Chemopreventive Isothiocyanates

Author:: Yuesheng Zhang
Source:: Critical reviews in food science and nutrition 2012 v.52 no.6 pp. 525-532
ISSN:: 1549-7852
Subject:: chemoprevention; humans; isothiocyanates; mechanism of action; metabolites; vegetables
Abstract:: ... Many naturally occurring isothiocyanates (ITCs) show highly promising chemopreventive activities. Humans are commonly exposed to these compounds through the consumption of cruciferous vegetables which are the main source of dietary ITCs. Dietary ITCs may play an important role in cancer prevention and in the well-recognized cancer preventive activities of cruciferous vegetables. A generic analytic ...
DOI:: 10.1080/10408398.2010.503288
PubMed:: 22452732
PubMed Central:: PMC3315191
: https://doi.org/10.1080/10408398.2010.503288

7. 1,4-Diazaspiro[2.2]pentanes as a Flexible Platform for the Synthesis of Diamine-Bearing Stereotriads

Author:: Rigoli Jared W.; Boralsky Luke A.; Hershberger John C.; Marston Dagmara; Meis Alan R.; Guzei Ilia A.; Schomaker Jennifer M.
Source:: Journal of organic chemistry 2012 v.77 no.5 pp. 2446-2455
ISSN:: 1520-6904
Subject:: Lewis bases; alcohols; allene; aziridines; bioactive compounds; chemical structure; optical isomerism; organic chemistry; oxidation; regioselectivity
Abstract:: ... Nitrogen-containing stereotriads occur in a number of biologically active compounds, but general and flexible methods to access these compounds are limited mainly to the manipulation of chiral olefins. An alternative approach is to employ a highly chemo-, regio-, and stereocontrolled allene oxidation that can install a new carbon–heteroatom bond at each of the three original allene carbons. In thi ...
DOI:: 10.1021/jo3000282
PubMed:: 22304460
PubMed Central:: PMC4500119
: https://doi.org/10.1021/jo3000282

8. 1,500 year quantitative reconstruction of winter precipitation in the Pacific Northwest

Author:: Byron A. Steinman; Mark B. Abbott; Michael E. Mann; Nathan D. Stansell; Bruce P. Finney
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.29 pp. 11619-11623
ISSN:: 0027-8424
Subject:: El Nino; climate; drought; growth rings; isotopes; lakes; oxygen; paleoclimatology; seasonal variation; sediments; winter
Abstract:: ... Multiple paleoclimate proxies are required for robust assessment of past hydroclimatic conditions. Currently, estimates of drought variability over the past several thousand years are based largely on tree-ring records. We produced a 1,500-y record of winter precipitation in the Pacific Northwest using a physical model-based analysis of lake sediment oxygen isotope data. Our results indicate that ...
DOI:: 10.1073/pnas.1201083109
PubMed:: 22753510
PubMed Central:: PMC3406856
: https://doi.org/10.1073/pnas.1201083109

9. 1-Methylthio-d-xylulose 5-Phosphate Methylsulfurylase: A Novel Route to 1-Deoxy-d-xylulose 5-Phosphate in Rhodospirillum rubrum

Author:: Warlick Benjamin P.; Evans Bradley S.; Erb Tobias J.; Ramagopal Udipi A.; Sriram Jaya; Imker Heidi J.; Sauder J. Michael; Bonanno Jeffrey B.; Burley Stephen K.; Tabita F. Robert; Almo Steven C.; Sweedler Jonathan S.; Gerlt John A.
Source:: Biochemistry 2012 v.51 no.42 pp. 8324-8326
ISSN:: 1520-4995
Subject:: Rhodospirillum rubrum; S-adenosylmethionine; active sites; adenine; biosynthesis; dimethyl disulfide; isomerization; isoprenoids; polyamines
Abstract:: ... Rhodospirillum rubrum produces 5-methylthioadenosine (MTA) from S-adenosylmethionine in polyamine biosynthesis; however, R. rubrum lacks the classical methionine salvage pathway. Instead, MTA is converted to 5-methylthio-d-ribose 1-phosphate (MTR 1-P) and adenine; MTR 1-P is isomerized to 1-methylthio-d-xylulose 5-phosphate (MTXu 5-P) and reductively dethiomethylated to 1-deoxy-d-xylulose 5-phosph ...
DOI:: 10.1021/bi301215g
PubMed:: 23035785
PubMed Central:: PMC3490199
: https://doi.org/10.1021/bi301215g

10. The 10-Year Cost-Effectiveness of Lifestyle Intervention or Metformin for Diabetes Prevention: An intent-to-treat analysis of the DPP/DPPOS

Author:
Source:: Diabetes care 2012 v.35 no.4 pp. 723-730
ISSN:: 0149-5992
Subject:: adults; cost effectiveness; lifestyle; metformin; noninsulin-dependent diabetes mellitus; quality of life; quality-adjusted life year
Abstract:: ... OBJECTIVE: The Diabetes Prevention Program (DPP) and its Outcomes Study (DPPOS) demonstrated that either intensive lifestyle intervention or metformin could prevent type 2 diabetes in high-risk adults for at least 10 years after randomization. We report the 10-year within-trial cost-effectiveness of the interventions. RESEARCH DESIGN AND METHODS: Data on resource utilization, cost, and quality of ...
DOI:: 10.2337/dc11-1468
PubMed:: 22442395
PubMed Central:: PMC3308273
: https://doi.org/10.2337/dc11-1468

11. 100% Orange juice consumption is associated with better diet quality, improved nutrient adequacy, decreased risk for obesity, and improved biomarkers of health in adults: National Health and Nutrition Examination Survey, 2003-2006

Author:: Carol E O’Neil; Theresa A Nicklas; Gail C Rampersaud; Victor L Fulgoni III
Source:: Nutrition journal 2012 v.11 no.1 pp. 565
ISSN:: 1475-2891
Subject:: Adequate Intakes; food groups; odds ratio; folic acid; nutrients; adults; Estimated Average Requirement; biomarkers; orange juice; metabolic syndrome; risk factors; potassium; childhood obesity; USDA; body mass index; vitamin A; ascorbic acid; fruits; nutritional adequacy; regression analysis; magnesium; cholesterol; healthy diet; fruit consumption; National Health and Nutrition Examination Survey; whole grain foods
Abstract:: ... BACKGROUND: Consumption of 100% orange juice (OJ) has been positively associated with nutrient adequacy and diet quality, with no increased risk of overweight/obesity in children; however, no one has examined these factors in adults. The purpose of this study was to examine the association of 100% OJ consumption with nutrient adequacy, diet quality, and risk factors for metabolic syndrome (MetS) i ...
DOI:: 10.1186/1475-2891-11-107
PubMed:: 23234248
PubMed Central:: PMC3545988
: https://doi.org/10.1186/1475-2891-11-107

12. IL- 10–induced microRNA-187 negatively regulates TNF-α, IL-6, and IL-12p40 production in TLR4-stimulated monocytes

Author:: Marzia Rossato; Graziella Curtale; Nicola Tamassia; Monica Castellucci; Laura Mori; Sara Gasperini; Barbara Mariotti; Mariacristina De Luca; Massimiliano Mirolo; Marco A. Cassatella; Massimo Locati; Flavia Bazzoni
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.45 pp. E3101
ISSN:: 0027-8424
Subject:: Toll-like receptor 4; humans; interleukin-10; interleukin-12; interleukin-6; messenger RNA; microRNA; monocytes; physiological regulation; transcription (genetics); transcription factors; translation (genetics); tumor necrosis factor-alpha
Abstract:: ... IL-10 is a potent anti-inflammatory molecule that, in phagocytes, negatively targets cytokine expression at transcriptional and posttranscriptional levels. Posttranscriptional checkpoints also represent the specific target of a recently discovered, evolutionary conserved class of small silencing RNAs known as “microRNAs” (miRNAs), which display the peculiar function of negatively regulating mRNA p ...
DOI:: 10.1073/pnas.1209100109
PubMed:: 23071313
PubMed Central:: PMC3494907
: https://doi.org/10.1073/pnas.1209100109

13. 15N-Labeled Brain Enables Quantification of Proteome and Phosphoproteome in Cultured Primary Neurons

Author:: Liao Lujian; Sando Richard C.; Farnum John B.; Vanderklish Peter W.; Maximov Anton; Yates John R.
Source:: Journal of Proteome Research 2012 v.11 no.2 pp. 1341-1353
ISSN:: 1535-3907
Subject:: brain; models; neurons; phosphopeptides; phosphorylation; proteins; proteome; proteomics; quantitative analysis; stable isotopes; synapse; synaptic transmission
Abstract:: ... Terminally differentiated primary cells represent a valuable in vitro model to study signaling events associated within a specific tissue. Quantitative proteomic methods using metabolic labeling in primary cells encounter labeling efficiency issues hindering the use of these cells. Here we developed a method to quantify the proteome and phosphoproteome of cultured neurons using ¹⁵N-labeled brain t ...
DOI:: 10.1021/pr200987h
PubMed:: 22070516
PubMed Central:: PMC3279198
: https://doi.org/10.1021/pr200987h

14. 15N-Labeled Full-Length Apolipoprotein E4 as an Internal Standard for Mass Spectrometry Quantification of Apolipoprotein E Isoforms

Author:: Wang Meiyao; Chen Junjun; Turko Illarion V.
Source:: Analytical chemistry 2012 v.84 no.19 pp. 8340-8344
ISSN:: 1520-6882
Subject:: Alzheimer disease; alleles; amino acids; apolipoprotein E4; brain; genotype; humans; mass spectrometry; quantitative analysis; risk factors
Abstract:: ... Apolipoprotein E isoforms (apoE2, apoE3, and apoE4) affect the likelihood of developing Alzheimer’s disease (AD), with the apoE4 isoform being a major risk factor. However, the underlying mechanism remains to be determined. ApoE isoforms vary by a single amino acid change, and it is a challenge to distinguish them on the protein level. We developed a mass spectrometry-based quantitative method uti ...
DOI:: 10.1021/ac3018873
PubMed:: 22931427
PubMed Central:: PMC4038910
: https://doi.org/10.1021/ac3018873

15. 17 novel polymorphic microsatellite markers for the giant water bug, Abedus herberti (Belostomatidae)

Author:: T. S. Daly-Engel; R. L. Smith; D. S. Finn; M. E. Knoderbane; I. C. Phillipsen; D. A. Lytle
Source:: Conservation genetics resources 2012 v.4 no.4 pp. 979-981
ISSN:: 1877-7252
Subject:: Lethocerus americanus; alleles; aquatic habitat; climate change; evolution; groundwater; insects; loci; mating systems; microsatellite repeats
Abstract:: ... The giant water bug (Abedus herberti) is a large flightless insect that is a keystone predator in aridland aquatic habitats. Extended droughts, possibly due to climate change and groundwater pumping, are causing once-perennial aquatic habitats to dry, resulting in serious conservation concern for some populations. A. herberti also exhibits exclusive male parental care, which has made it a model or ...
DOI:: 10.1007/s12686-012-9687-5
PubMed:: 24077753
PubMed Central:: PMC3784353
: https://doi.org/10.1007/s12686-012-9687-5

16. 1H Dynamic Nuclear Polarization Based on an Endogenous Radical

Author:: Maly Thorsten; Cui Dongtao; Griffin Robert G.; Miller Anne-Frances
Source:: Journal of physical chemistry 2012 v.116 no.24 pp. 7055-7065
ISSN:: 1520-6106
Subject:: flavins; free radicals; nuclear magnetic resonance spectroscopy; organelles; physical chemistry; proteins
Abstract:: ... We demonstrate a 15-fold enhancement of solid-state NMR signals via dynamic nuclear polarization (DNP) based on a stable, naturally occurring radical in a protein: the flavin mononucleotide (FMN) semiquinone of flavodoxin. The line width of flavodoxin’s EPR signal suggests that the dominant DNP mechanism is the solid effect, consistent with the field-dependent DNP enhancement profile. The magnitud ...
DOI:: 10.1021/jp300539j
PubMed:: 22472179
PubMed Central:: PMC6010065
: https://doi.org/10.1021/jp300539j

17. 1α,25-Dihydroxyvitamin D₃ and its analog, 2-methylene-19-nor-(20S)-1α,25-dihydroxyvitamin D₃ (2MD), suppress intraocular pressure in non-human primates

Author:: Galina D. Kutuzova; B’Ann T. Gabelt; Julie A. Kiland; Elizabeth A. Hennes-Beann; Paul L. Kaufman; Hector F. DeLuca
Source:: Archives of biochemistry and biophysics 2012 v.518 no.1 pp. 53-60
ISSN:: 0003-9861
Subject:: Primates; drainage; genes; glaucoma; hypertension; microarray technology; people; risk factors; therapeutics
Abstract:: ... Ocular hypertension is the greatest known risk factor for glaucoma that affects an estimated 70million people worldwide. Lowering intraocular pressure (IOP) remains the mainstay of therapy in the management of glaucoma. By means of microarray analysis, we have discovered that 1α,25-dihydroxyvitamin D₃ (1α,25-(OH)₂D₃) regulates genes that are known to be involved in the determination of intraocular ...
DOI:: 10.1016/j.abb.2011.10.022
PubMed:: 22198282
PubMed Central:: PMC3390938
: https://doi.org/10.1016/j.abb.2011.10.022

18. 1’-Acetoxychavicol acetate inhibits growth of human oral carcinoma xenograft in mice and potentiates cisplatin effect via proinflammatory microenvironment alterations

Author:: Lionel LA In; Norhafiza M Arshad; Halijah Ibrahim; Mohamad Nurul Azmi; Khalijah Awang; Noor Hasima Nagoor
Source:: BMC complementary and alternative medicine 2012 v.12 no.1 pp. 179
ISSN:: 1472-6882
Subject:: DNA fragmentation; Western blotting; acetates; alternative medicine; apoptosis; biomarkers; body weight changes; cisplatin; clinical trials; cyclins; cytotoxicity; genes; ginger; humans; mechanism of action; mice; mouth neoplasms; squamous cell carcinoma; survival rate; transcription factor NF-kappa B
Abstract:: ... BACKGROUND: Oral cancers although preventable, possess a low five-year survival rate which has remained unchanged over the past three decades. In an attempt to find a more safe, affordable and effective treatment option, we describe here the use of 1’S-1’-acetoxychavicol acetate (ACA), a component of Malaysian ginger traditionally used for various medicinal purposes. METHODS: Whether ACA can inhib ...
DOI:: 10.1186/1472-6882-12-179
PubMed:: 23043547
PubMed Central:: PMC3517351
: https://doi.org/10.1186/1472-6882-12-179

19. 2,2′,3,5′,6-Pentachlorobiphenyl (PCB 95) and Its Hydroxylated Metabolites Are Enantiomerically Enriched in Female Mice

Author:: Kania-Korwel Izabela; Barnhart Christopher D.; Stamou Marianna; Truong Kim M.; El-Komy Mohammed H. M. E.; Lein Pamela J.; Veng-Pedersen Peter; Lehmler Hans-Joachim
Source:: Environmental Science & Technology 2012 v.46 no.20 pp. 11393-11401
ISSN:: 1520-5851
Subject:: adults; blood; brain; dose response; females; liver; metabolites; mice; neurotoxicity; polychlorinated biphenyls; subchronic exposure; tissues
Abstract:: ... Epidemiological and laboratory studies link polychlorinated biphenyls and their metabolites to adverse neurodevelopmental outcomes. Several neurotoxic PCB congeners are chiral and undergo enantiomeric enrichment in mammalian species, which may modulate PCB developmental neurotoxicity. This study measures levels and enantiomeric enrichment of PCB 95 and its hydroxylated metabolites (OH-PCBs) in adu ...
DOI:: 10.1021/es302810t
PubMed:: 22974126
PubMed Central:: PMC3475189
: https://doi.org/10.1021/es302810t

20. 2,3-Hexanediols as Sex Attractants and a Female-produced Sex Pheromone for Cerambycid Beetles in the Prionine Genus Tragosoma

Author:: Ann M. Ray; James D. Barbour; J. Steven McElfresh; Jardel A. Moreira; Ian Swift; Ian M. Wright; Alenka Žunič; Robert F. Mitchell; Elizabeth E. Graham; Ronald L. Alten; Jocelyn G. Millar; Lawrence M. Hanks
Source:: Journal of chemical ecology 2012 v.38 no.9 pp. 1151-1158
ISSN:: 0098-0331
Subject:: Cerambycidae; aggregation pheromones; bioassays; females; fresh produce; males; pheromone blends; pheromone glands; sex attractants; sex pheromones; sexual dimorphism; California
Abstract:: ... Recent work suggests that closely related cerambycid species often share pheromone components, or even produce pheromone blends of identical composition. However, little is known of the pheromones of species in the subfamily Prioninae. During field bioassays in California, males of three species in the prionine genus Tragosoma were attracted to 2,3-hexanediols, common components of male-produced a ...
DOI:: 10.1007/s10886-012-0181-z
PubMed:: 22923142
PubMed Central:: PMC3808253
: https://doi.org/10.1007/s10886-012-0181-z

21. The 21.5-kDa isoform of myelin basic protein has a non-traditional PY-nuclear-localization signal

Author:: Graham S.T. Smith; Lauren V. Seymour; Joan M. Boggs; George Harauz
Source:: Biochemical and biophysical research communications 2012 v.422 no.4 pp. 670-675
ISSN:: 0006-291X
Subject:: actin; arginine; central nervous system; fluorescence microscopy; fluorescent proteins; genes; lysine; myelin basic protein; myelin sheath; protein transport; site-directed mutagenesis; tubulin
Abstract:: ... The predominant 18.5-kDa classic myelin basic protein (MBP) is mainly responsible for compaction of the myelin sheath in the central nervous system, but is multifunctional, having numerous interactions with Ca²⁺-calmodulin, actin, tubulin, and SH3-domains, and can tether these proteins to a lipid membrane in vitro. The full-length 21.5-kDa MBP isoform has an additional 26 residues encoded by exon- ...
DOI:: 10.1016/j.bbrc.2012.05.051
PubMed:: 22609403
PubMed Central:: PMC3526657
: https://doi.org/10.1016/j.bbrc.2012.05.051

22. 25-Hydroxyvitamin D Concentrations and In Vivo Insulin Sensitivity and β-Cell Function Relative to Insulin Sensitivity in Black and White Youth

Author:: Kumaravel Rajakumar; Javier de las Heras; SoJung Lee; Michael F. Holick; Silva A. Arslanian
Source:: Diabetes care 2012 v.35 no.3 pp. 627-633
ISSN:: 0149-5992
Subject:: Blacks; Whites; adipose tissue; adiposity; body mass index; body measurements; fasting; glucose; homeostasis; insulin; insulin resistance; secretion; vitamin D; volunteers; youth
Abstract:: ... OBJECTIVE: To examine the relationships between plasma 25-hydroxyvitamin D [25(OH)D] and in vivo insulin sensitivity and β-cell function relative to insulin sensitivity, disposition index (DI), in black and white youth. RESEARCH DESIGN AND METHODS: Plasma 25(OH)D concentrations were analyzed in banked specimens in healthy youth aged 8 to 18 years who had existing data on hyperinsulinemic-euglycemi ...
DOI:: 10.2337/dc11-1825
PubMed:: 22238280
PubMed Central:: PMC3322701
: https://doi.org/10.2337/dc11-1825

23. 25-Hydroxyvitamin D deficiency is associated with fatal stroke among whites but not blacks: The NHANES-III linked mortality files

Author:: Erin D. Michos; Jared P. Reis; Wendy S. Post; Pamela L. Lutsey; Rebecca F. Gottesman; Thomas H. Mosley; A. Richey Sharrett; Michal L. Melamed
Source:: Nutrition 2012 v.28 no.4 pp. 367-371
ISSN:: 0899-9007
Subject:: risk factors; regression analysis; confidence interval; socioeconomic status; Whites; stroke; vitamin deficiencies; Blacks; National Health and Nutrition Examination Survey; mortality; adults; United States
Abstract:: ... OBJECTIVE: Deficient 25-hydroxyvitamin D (25[OH]D) levels are associated with cardiovascular disease (CVD) events and mortality. 25(OH)D deficiency and stroke are more prevalent in blacks. We examined whether low 25(OH)D contributes to the excess risk of fatal stroke in blacks compared with whites. METHODS: The Third National Health and Nutrition Examination Survey, a probability sample of U.S. ci ...
DOI:: 10.1016/j.nut.2011.10.015
PubMed:: 22261577
PubMed Central:: PMC3304002
: https://doi.org/10.1016/j.nut.2011.10.015

24. 26S Proteasome regulation of Ankrd1/CARP in adult rat ventricular myocytes and human microvascular endothelial cells

Author:: Susan E. Samaras; Billy Chen; Stephen R. Koch; Douglas B. Sawyer; Chee Chew Lim; Jeffrey M. Davidson
Source:: Biochemical and biophysical research communications 2012 v.425 no.4 pp. 830-835
ISSN:: 0006-291X
Subject:: proteasome endopeptidase complex; messenger RNA; tissue repair; endothelial cells; humans; muscles; sarcomeres; proteolysis; rats; adults; half life
Abstract:: ... Ankyrin repeat domain 1 protein (Ankrd1), also known as cardiac ankyrin repeat protein (CARP), increases dramatically after tissue injury, and its overexpression improves aspects of wound healing. Reports that Ankrd1/CARP protein stability may affect cardiovascular organization, together with our findings that the protein is crucial to stability of the cardiomyocyte sarcomere and increased in woun ...
DOI:: 10.1016/j.bbrc.2012.07.162
PubMed:: 22892129
PubMed Central:: PMC3460693
: https://doi.org/10.1016/j.bbrc.2012.07.162

25. The 27–year decline of coral cover on the Great Barrier Reef and its causes

Author:: Glenn De’ath; Katharina E. Fabricius; Hugh Sweatman; Marji Puotinen
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.44 pp. 17995-17999
ISSN:: 0027-8424
Subject:: Acanthaster planci; bleaching; climatic factors; control methods; coral bleaching; coral reefs; corals; hurricanes; mortality; predation; surveys; temporal variation; time series analysis; water quality; Great Barrier Reef
Abstract:: ... The world’s coral reefs are being degraded, and the need to reduce local pressures to offset the effects of increasing global pressures is now widely recognized. This study investigates the spatial and temporal dynamics of coral cover, identifies the main drivers of coral mortality, and quantifies the rates of potential recovery of the Great Barrier Reef. Based on the world’s most extensive time s ...
DOI:: 10.1073/pnas.1208909109
PubMed:: 23027961
PubMed Central:: PMC3497744
: https://doi.org/10.1073/pnas.1208909109

26. The 2b proteins of Cucumber mosaic virus generally have the potential to differentially induce necrosis on Arabidopsis

Author:: Chikara Masuta; Jun-ichi Inaba; Hanako Shimura
Source:: Plant signaling & behavior 2012 v.7 no.1 pp. 43-45
ISSN:: 1559-2324
Subject:: Arabidopsis; Cucumber mosaic virus; RNA interference; catalase; enzyme activity; hydrogen peroxide; necrosis; proteins; sequence homology; virulent strains
Abstract:: ... Plant viral symptoms are rarely explained by direct molecular interaction between a viral protein and a host factor, but rather understood as a consequence of arms race between host RNA silencing and viral silencing suppressors. However, we have recently demonstrated that the 2b protein (2b) of Cucumber mosaic virus (CMV) HL strain could bind to Arabidopsis catalase that is important in scavenging ...
DOI:: 10.4161/psb.7.1.18526
PubMed:: 22301966
PubMed Central:: PMC3357366
: https://doi.org/10.4161/psb.7.1.18526

27. 3-Helix Micelles Stabilized by Polymer Springs

Author:: Dong He; Shu Jessica Y.; Dube Nikhil; Ma Yufei; Tirrell Matthew V.; Downing Kenneth H.; Xu Ting
Source:: Journal of the American Chemical Society 2012 v.134 no.28 pp. 11807-11814
ISSN:: 1520-5126
Subject:: chemical bonding; colloids; deformation; desorption; energy; hydrophilicity; micelles; nanocarriers; particle size; polyethylene glycol
Abstract:: ... Despite increasing demands to employ amphiphilic micelles as nanocarriers and nanoreactors, it remains a significant challenge to simultaneously reduce the particle size and enhance the particle stability. Complementary to covalent chemical bonding and attractive intermolecular interactions, entropic repulsion can be incorporated by rational design in the headgroup of an amphiphile to generate sma ...
DOI:: 10.1021/ja3048128
PubMed:: 22731391
PubMed Central:: PMC4186246
: https://doi.org/10.1021/ja3048128

28. IL- 33 is processed into mature bioactive forms by neutrophil elastase and cathepsin G

Author:: Emma Lefrançais; Stephane Roga; Violette Gautier; Anne Gonzalez-de-Peredo; Bernard Monsarrat; Jean-Philippe Girard; Corinne Cayrol
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.5 pp. 1673-1678
ISSN:: 0027-8424
Subject:: asthma; basophils; bioactive properties; cardiovascular diseases; caspase-1; cathepsin G; colitis; elastase; eosinophils; humans; inflammation; interleukin-1; interleukin-18; lymphocytes; mast cells; mice; neutrophils; rheumatoid arthritis
Abstract:: ... Interleukin-33 (IL-33) (NF-HEV) is a chromatin-associated nuclear cytokine from the IL-1 family, which has been linked to important diseases, including asthma, rheumatoid arthritis, ulcerative colitis, and cardiovascular diseases. IL-33 signals through the ST2 receptor and drives cytokine production in type 2 innate lymphoid cells (ILCs) (natural helper cells, nuocytes), T-helper (Th)2 lymphocytes ...
DOI:: 10.1073/pnas.1115884109
PubMed:: 22307629
PubMed Central:: PMC3277172
: https://doi.org/10.1073/pnas.1115884109

29. IL- 38 binds to the IL-36 receptor and has biological effects on immune cells similar to IL-36 receptor antagonist

Author:: Frank L. van de Veerdonk; Angela K. Stoeckman; Gouping Wu; Aaron N. Boeckermann; Tania Azam; Mihai G. Netea; Leo A. B. Joosten; Jos W. M. van der Meer; Ruyi Hao; Vassili Kalabokis; Charles A. Dinarello
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.8 pp. 3001-3005
ISSN:: 0027-8424
Subject:: Candida albicans; amino acids; antagonists; dendritic cells; humans; interleukin-1; interleukin-6; interleukin-8; mononuclear leukocytes
Abstract:: ... The functional role of IL-1 family member 10, recently renamed IL-38, remains unknown. In the present study we aimed to elucidate the biological function of IL-38 and to identify its receptor. Heat-killed Candida albicans was used to stimulate memory T-lymphocyte cytokine production in freshly obtained human peripheral blood mononuclear cells from healthy subjects. The addition of recombinant IL-3 ...
DOI:: 10.1073/pnas.1121534109
PubMed:: 22315422
PubMed Central:: PMC3286950
: https://doi.org/10.1073/pnas.1121534109

30. 3D imaging and mechanical modeling of helical buckling in Medicago truncatula plant roots

Author:: Jesse L. Silverberg; Roslyn D. Noar; Michael S. Packer; Maria J. Harrison; Christopher L. Henley; Itai Cohen; Sharon J. Gerbode
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.42 pp. 16794-16799
ISSN:: 0027-8424
Subject:: Medicago truncatula; culture media; deformation; gels; hydrocolloids; image analysis; mathematical models; root growth; roots
Abstract:: ... We study the primary root growth of wild-type Medicago truncatula plants in heterogeneous environments using 3D time-lapse imaging. The growth medium is a transparent hydrogel consisting of a stiff lower layer and a compliant upper layer. We find that the roots deform into a helical shape just above the gel layer interface before penetrating into the lower layer. This geometry is interpreted as a ...
DOI:: 10.1073/pnas.1209287109
PubMed:: 23010923
PubMed Central:: PMC3479456
: https://doi.org/10.1073/pnas.1209287109

31. A 3D localized surface plasmon resonance biosensor for the study of trivalent arsenic binding to the ArsA ATPase

Author:: Chang Liu; Vittoria Balsamo; Dali Sun; Melodie Naja; Xuemei Wang; Barry Rosen; Chen-Zhong Li
Source:: Biosensors & bioelectronics 2012 v.38 no.1 pp. 19-26
ISSN:: 0956-5663
Subject:: Escherichia coli; acrylic acid; adenosine triphosphate; adenosinetriphosphatase; arsenic; arsenites; binding sites; biosensors; magnesium; nanogold; operon; plasmids; protein subunits; proteins; surface plasmon resonance
Abstract:: ... A self-assembled 3D hydrogel–nanoparticle composite integrated surface plasmon resonance (SPR) sensor is reported here. The novel assembled substrate was developed by means of a surface mediated radical co-polymerization process to obtain a highly sensitive hydrogel-based thin film that possesses specific binding sites for target analytes. Initially, amino group modified gold nanoparticles (AuNPs) ...
DOI:: 10.1016/j.bios.2012.04.026
PubMed:: 22658909
PubMed Central:: PMC3715140
: https://doi.org/10.1016/j.bios.2012.04.026

32. 3D superhydrophobic electrospun meshes as reinforcement materials for sustained local drug delivery against colorectal cancer cells

Author:: Stefan T. Yohe; Victoria L.M. Herrera; Yolonda L. Colson; Mark W. Grinstaff
Source:: Journal of controlled release 2012 v.162 no.1 pp. 92-101
ISSN:: 0168-3659
Subject:: air; antineoplastic agents; colorectal neoplasms; cytotoxicity; drug therapy; glycerol; humans; hydrophobicity; mechanical properties; neoplasm cells; polymers; ultrasonics
Abstract:: ... In this work we expand upon a recently reported local drug delivery device, where air is used as a degradable component of our material to control drug release (J. Am. Chem. Soc. 2012, 134, 2016–2019). We consider its potential use as a drug loaded strip to provide both mechanical stability to the anastomosis, and as a means to release drug locally over prolonged periods for prevention of locoregi ...
DOI:: 10.1016/j.jconrel.2012.05.047
PubMed:: 22684120
PubMed Central:: PMC3878861
: https://doi.org/10.1016/j.jconrel.2012.05.047

33. 4-Hydroxynonenal differentially regulates adiponectin gene expression and secretion via activating PPARγ and accelerating ubiquitin–proteasome degradation

Author:: Zhigang Wang; Xiaobing Dou; Dongfang Gu; Chen Shen; Tong Yao; Van Nguyen; Carol Braunschweig; Zhenyuan Song
Source:: Molecular and cellular endocrinology 2012 v.349 no.2 pp. 222-231
ISSN:: 0303-7207
Subject:: adipocytes; adiponectin; adipose tissue; antagonists; antioxidants; betaine; cycloheximide; dose response; gene expression; high fat diet; insulin resistance; lipid peroxidation; metabolic diseases; mice; obesity; oxidative stress; precipitin tests; proteasome endopeptidase complex; protein degradation; secretion
Abstract:: ... Although well-established, the underlying mechanisms involved in obesity-related plasma adiponectin decline remain elusive. Oxidative stress is associated with obesity and insulin resistance and considered to contribute to the progression toward obesity-related metabolic disorders. In this study, we investigated the effects of 4-hydroxynonenal (4-HNE), the most abundant lipid peroxidation end prod ...
DOI:: 10.1016/j.mce.2011.10.027
PubMed:: 22085560
PubMed Central:: PMC3594100
: https://doi.org/10.1016/j.mce.2011.10.027

34. 4-O-methylation of glucuronic acid in Arabidopsis glucuronoxylan is catalyzed by a domain of unknown function family 579 protein

Author:: Breeanna R. Urbanowicz; Maria J. Peña; Supriya Ratnaparkhe; Utku Avci; Jason Backe; Heather F. Steet; Marcus Foston; Hongjia Li; Malcolm A. O’Neill; Arthur J. Ragauskas; Alan G. Darvill; Charles Wyman; Harry J. Gilbert; William S. York
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.35 pp. 14253-14258
ISSN:: 0027-8424
Subject:: physical properties; glucuronic acid; Arabidopsis; cobalt; proteins; xylan; biopolymers; cell walls; biochemical mechanisms; hot water treatment; lignin; methyltransferases; Golgi apparatus
Abstract:: ... The hemicellulose 4- O -methyl glucuronoxylan is one of the principle components present in the secondary cell walls of eudicotyledonous plants. However, the biochemical mechanisms leading to the formation of this polysaccharide and the effects of modulating its structure on the physical properties of the cell wall are poorly understood. We have identified and functionally characterized an Arabido ...
DOI:: 10.1073/pnas.1208097109
PubMed:: 22893684
PubMed Central:: PMC3435161
: https://doi.org/10.1073/pnas.1208097109

35. 470-Million-year-old black corals from China

Author:: Andrzej Baliński; Yuanlin Sun; Jerzy Dzik
Source:: Naturwissenschaften 2012 v.99 no.8 pp. 645-653
ISSN:: 0028-1042
Subject:: Anthozoa; chitin; corals; fossils; phylogeny; prediction; China
Abstract:: ... Phosphatic (possibly secondarily phosphatised) remains of antipatharian coralla, previously unknown in the fossil record, occur abundantly in the early Ordovician Fenxiang Formation in the Hubei Province, southern China. Probably two species (and genera) are represented, which differ in spinosity of branches. The more spinose one, Sinopathes reptans, has its lateral spines bearing regular, longitu ...
DOI:: 10.1007/s00114-012-0947-8
PubMed:: 22790835
PubMed Central:: PMC3407557
: https://doi.org/10.1007/s00114-012-0947-8

36. 5-Carboxylcytosine is localized to euchromatic regions in the nuclei of follicular cells in axolotl ovary

Author:: Anthony Alioui; Lee M. Wheldon; Abdulkadir Abakir; Zoltan Ferjentsik; Andrew D. Johnson; Alexey Ruzov
Source:: Nucleus 2012 v.3 no.6 pp. 565-569
ISSN:: 1949-1042
Subject:: 5-methylcytosine; demethylation; epigenetics; genes; mice
Abstract:: ... 5-Methylcytosine (5-mC) is an epigenetic modification associated with gene repression. Recent studies demonstrated that 5-mC can be enzymatically oxidised into 5-hydroxymethylcytosine and further into 5-formylcytosine (5-fC) and 5-carboxylcytsine (5-caC). 5-caC has been found in embryonic stem cells and in mouse pre-implantation embryos but no detectable levels of this modification have been repor ...
DOI:: 10.4161/nucl.22799
PubMed:: 23138778
PubMed Central:: PMC3515539
: https://doi.org/10.4161/nucl.22799

37. 5-Hydroxymethylcytosine – the elusive epigenetic mark in mammalian DNA

Author:: Edita Kriukienė; Zita Liutkevičiūtė; Saulius Klimašauskas
Source:: Chemical Society reviews 2012 v.41 no.21 pp. 6916-6930
ISSN:: 1460-4744
Subject:: DNA; DNA demethylation; DNA methylation; brain; cell differentiation; embryogenesis; embryonic stem cells; epigenetics; genome; human diseases; humans; iron; mice; neurons; nucleobases; oxygenases
Abstract:: ... Over the past decade, epigenetic phenomena claimed a central role in cell regulatory processes and proved to be important factors for understanding complex human diseases. One of the best understood epigenetic mechanisms is DNA methylation. In the mammalian genome, cytosines (C) were long known to exist in two functional states: unmethylated or methylated at the 5-position of the pyrimidine ring ( ...
DOI:: 10.1039/c2cs35104h
PubMed:: 22842880
PubMed Central:: PMC3467341
: https://doi.org/10.1039/c2cs35104h

38. 500,000 fish phenotypes: The new informatics landscape for evolutionary and developmental biology of the vertebrate skeleton

Author:: P. Mabee; J. P. Balhoff; W. M. Dahdul; H. Lapp; P. E. Midford; T. J. Vision; M. Westerfield
Source:: Journal of applied ichthyology 2012 v.28 no.3 pp. 300-305
ISSN:: 0175-8659
Subject:: Danio rerio; evolution; fish; genes; landscapes; phenotype; skeleton
Abstract:: ... The rich phenotypic diversity that characterizes the vertebrate skeleton results from evolutionary changes in regulation of genes that drive development. Although relatively little is known about the genes that underlie the skeletal variation among fish species, significant knowledge of genetics and development is available for zebrafish. Because developmental processes are highly conserved, this ...
DOI:: 10.1111/j.1439-0426.2012.01985.x
PubMed:: 22736877
PubMed Central:: PMC3377363
: https://doi.org/10.1111/j.1439-0426.2012.01985.x

39. A 54-Kilodalton Protein Encoded by pBtoxis Is Required for Parasporal Body Structural Integrity in Bacillus thuringiensis subsp. israelensis

Author:: Mercedes Diaz-Mendoza; Dennis K. Bideshi; Brian A. Federici
Source:: Journal of bacteriology 2012 v.194 no.6 pp. 1562-1571
ISSN:: 0021-9193
Subject:: Bacillus thuringiensis subsp. israelensis; cytoplasm; endospores; endotoxins; fluorescence microscopy; gene expression; insect larvae; molecular biology; peptides; plasmids; proteins; proteomics; sporulation; toxicity
Abstract:: ... Strains of Bacillus thuringiensis such as B. thuringiensis subsp. israelensis (ONR-60A) and B. thuringiensis subsp. morrisoni (PG-14) pathogenic for mosquito larvae produce a complex parasporal body consisting of several protein endotoxins synthesized during sporulation that form an aggregate of crystalline inclusions bound together by a multilamellar fibrous matrix. Most studies of these strains ...
DOI:: 10.1128/JB.06095-11
PubMed:: 22210770
PubMed Central:: PMC3294862
: https://doi.org/10.1128/JB.06095-11

40. 5′-Terminal chemical capping of spliced leader RNAs

Author:: Karolina Piecyk; Richard E. Davis; Marzena Jankowska-Anyszka
Source:: Tetrahedron letters 2012 v.53 no.36 pp. 4843-4847
ISSN:: 0040-4039
Subject:: Nematoda; biotin; chemical reactions; chemical structure; messenger RNA; mutants; nucleotides; organic compounds; trans-splicing; translation (genetics)
Abstract:: ... Spliced leader (SL) RNA trans-splicing adds a N²,N²,7-trimethylguanosine cap (TMG) and a 22-nucleotide sequence, the SL, to the 5′ end of mRNAs. Both non-trans-spliced with a monomethylguanosine cap (MMG) and trans-spliced mRNAs co-exist in trans-splicing metazoan cells. Efficient translation of TMG-capped mRNAs in nematodes requires a defined core of nucleotides within the SL sequence. Here we pr ...
DOI:: 10.1016/j.tetlet.2012.06.127
PubMed:: 23175583
PubMed Central:: PMC3501006
: https://doi.org/10.1016/j.tetlet.2012.06.127

41. 5′UTR-intron of the Gladiolus polyubiquitin promoter GUBQ1 enhances translation efficiency in Gladiolus and Arabidopsis

Author:: Kathryn Kamo; Ah-Young Kim; Se Hee Park; Young Hee Joung
Source:: BMC Plant Biology 2012 v.12 no. pp. 79-88
ISSN:: 1471-2229
Subject:: 5' untranslated regions; Arabidopsis thaliana; Gladiolus; Southern blotting; beta-glucuronidase; gene expression; genetic transformation; introns; leaves; promoter regions; reporter genes; roots; transgenic plants; translation (genetics); ubiquitin
Abstract:: ... There are many non-cereal monocots of agronomic, horticultural, and biofuel importance. Successful transformation of these species requires an understanding of factors controlling expression of their genes. Introns have been known to affect both the level and tissue-specific expression of genes in dicots and cereal monocots, but there have been no studies on an intron isolated from a non-cereal mo ...
Handle:: 10113/56980
DOI:: 10.1186/1471-2229-12-79
PubMed:: 22672685
PubMed Central:: PMC3406973
: https://doi.org/10.1186/1471-2229-12-79

42. The 60-Kilodalton Protein Encoded by orf2 in the cry19A Operon of Bacillus thuringiensis subsp. jegathesan Functions Like a C-Terminal Crystallization Domain

Author:: J. Eleazar Barboza-Corona; Hyun-Woo Park; Dennis K. Bideshi; Brian A. Federici
Source:: Applied and environmental microbiology 2012 v.78 no.6 pp. 2005-2012
ISSN:: 0099-2240
Subject:: Bacillus thuringiensis subsp. jegathesan; Culex quinquefasciatus; amino acid sequences; bioassays; crystallization; crystals; larvae; operon; proteins; sequence analysis; toxicity
Abstract:: ... The cry19A operon of Bacillus thuringiensis subsp. jegathesan encodes two proteins, mosquitocidal Cry19A (ORF1; 75 kDa) and an ORF2 (60 kDa) of unknown function. Expression of the cry19A operon in an acrystalliferous strain of B. thuringiensis (4Q7) yielded one small crystal per cell, whereas no crystals were produced when cry19A or orf2 was expressed alone. To determine the function of the ORF2 p ...
DOI:: 10.1128/AEM.06750-11
PubMed:: 22247140
PubMed Central:: PMC3298145
: https://doi.org/10.1128/AEM.06750-11

43. 6′-Oxa analogs of S-adenosylhomocysteine

Author:: Weikuan Li; Wei Ye; Stewart W. Schneller
Source:: Tetrahedron 2012 v.68 no.1 pp. 65-71
ISSN:: 0040-4020
Subject:: S-adenosylmethionine; chemical structure; methylation
Abstract:: ... S-Adenosylmethionine (AdoMet) is a ubiquitous cofactor in biomethylations and, in that role, becomes S-adenosylhomocysteine (AdoHcy), which serves as a biofeedback inhibitor of the methylation process. In seeking to avail unexplored structural variations of AdoHcy for biological studies, its 6′-oxa analog and two corresponding carbocyclic nucleosides (based on aristeromycin and neplanocin) have be ...
DOI:: 10.1016/j.tet.2011.10.063
PubMed:: 22489098
PubMed Central:: PMC3320718
: https://doi.org/10.1016/j.tet.2011.10.063

44. IL- 7 receptor blockade reverses autoimmune diabetes by promoting inhibition of effector/memory T cells

Author:: Cristina Penaranda; Wilson Kuswanto; Jerry Hofmann; Rupert Kenefeck; Parth Narendran; Lucy S. K. Walker; Jeffrey A. Bluestone; Abul K. Abbas; Hans Dooms
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.31 pp. 12668-12673
ISSN:: 0027-8424
Subject:: T-lymphocytes; animal disease models; autoimmune diseases; autoimmunity; death; diabetes mellitus; interferon-gamma; interleukin-7; mice; monoclonal antibodies; pathogenesis; therapeutics
Abstract:: ... To protect the organism against autoimmunity, self-reactive effector/memory T cells (T E/M) are controlled by cell-intrinsic and -extrinsic regulatory mechanisms. However, how some T E/M cells escape regulation and cause autoimmune disease is currently not understood. Here we show that blocking IL-7 receptor-α (IL-7Rα) with monoclonal antibodies in nonobese diabetic (NOD) mice prevented autoimmune ...
DOI:: 10.1073/pnas.1203692109
PubMed:: 22733744
PubMed Central:: PMC3411948
: https://doi.org/10.1073/pnas.1203692109

45. 7,8,3′-Trihydroxyflavone, a potent small molecule TrkB receptor agonist, protects spiral ganglion neurons from degeneration both in vitro and in vivo

Author:: Qing Yu; Qing Chang; Xia Liu; Shusheng Gong; Keqiang Ye; Xi Lin
Source:: Biochemical and biophysical research communications 2012 v.422 no.3 pp. 387-392
ISSN:: 0006-291X
Subject:: agonists; ears; ganglia; hearing disorders; mice; neurons; receptor protein-tyrosine kinase
Abstract:: ... Most sensorineural hearing loss cases occur as a result of hair cell loss, which results in secondary degeneration of spiral ganglion neurons (SGNs). Substantial loss of SGNs reduces the benefit of cochlear implants, which rely on SGNs for transmitting signals to the central auditory centers. Brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) play essential roles in cochlear develo ...
DOI:: 10.1016/j.bbrc.2012.04.154
PubMed:: 22575512
PubMed Central:: PMC3388121
: https://doi.org/10.1016/j.bbrc.2012.04.154

46. The 70S ribosome modulates the ATPase activity of Escherichia coli YchF

Author:: Marion Becker; Katherine E. Gzyl; Alvin M. Altamirano; Anthony Vuong; Kirstin Urbahn; Hans-Joachim Wieden
Source:: RNA biology 2012 v.9 no.10 pp. 1288-1301
ISSN:: 1555-8584
Subject:: Escherichia coli; RNA; adenosinetriphosphatase; catalytic activity; fluorescence; guanine nucleotides; guanosinetriphosphatase; humans; ribosomes
Abstract:: ... YchF is one of two universally conserved GTPases with unknown cellular function. As a first step toward elucidating YchF’s cellular role, we performed a detailed biochemical characterization of the protein from Escherichia coli. Our data from fluorescence titrations not only confirmed the surprising finding that YchFE.cₒₗᵢ binds adenine nucleotides more efficiently than guanine nucleotides, but al ...
DOI:: 10.4161/rna.22131
PubMed:: 22995830
PubMed Central:: PMC3583859
: https://doi.org/10.4161/rna.22131

47. 9,400 years of cosmic radiation and solar activity from ice cores and tree rings

Author:: Friedhelm Steinhilber; Jose A. Abreu; Jürg Beer; Irene Brunner; Marcus Christl; Hubertus Fischer; Ulla Heikkilä; Peter W. Kubik; Mathias Mann; Ken G. McCracken; Heinrich Miller; Hiroko Miyahara; Hans Oerter; Frank Wilhelms
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.16 pp. 5967-5971
ISSN:: 0027-8424
Subject:: climate; greenhouse gases; growth rings; ice; principal component analysis; radionuclides; solar radiation; temporal variation; volcanoes; Antarctica; Greenland
Abstract:: ... Understanding the temporal variation of cosmic radiation and solar activity during the Holocene is essential for studies of the solar-terrestrial relationship. Cosmic-ray produced radionuclides, such as 10Be and 14C which are stored in polar ice cores and tree rings, offer the unique opportunity to reconstruct the history of cosmic radiation and solar activity over many millennia. Although records ...
DOI:: 10.1073/pnas.1118965109
PubMed:: 22474348
PubMed Central:: PMC3341045
: https://doi.org/10.1073/pnas.1118965109

48. 9-Mercaptodethiobiotin Is Generated as a Ligand to the [2Fe–2S]+ Cluster during the Reaction Catalyzed by Biotin Synthase from Escherichia coli

Author:: Fugate Corey J.; Stich Troy A.; Kim Esther G.; Myers William K.; Britt R. David; Jarrett Joseph T.
Source:: Journal of the American Chemical Society 2012 v.134 no.22 pp. 9042-9045
ISSN:: 1520-5126
Subject:: Escherichia coli; S-adenosylmethionine; biotin synthase; electron paramagnetic resonance spectroscopy; free radicals; hydrogen; isotope labeling; ligands; models; sulfur
Abstract:: ... Biotin synthase catalyzes formation of the thiophane ring through stepwise substitution of a sulfur atom for hydrogen atoms at the C9 and C6 positions of dethiobiotin. Biotin synthase is a radical S-adenosylmethionine (SAM) enzyme that reductively cleaves S-adenosylmethionine, generating 5′-deoxyadenosyl radicals that initially abstract a hydrogen atom from the C9 position of dethiobiotin. We have ...
DOI:: 10.1021/ja3012963
PubMed:: 22607542
PubMed Central:: PMC3418058
: https://doi.org/10.1021/ja3012963

49. An A/ENTH Domain-Containing Protein Functions as an Adaptor for Clathrin-Coated Vesicles on the Growing Cell Plate in Arabidopsis Root Cells

Author:: Kyungyoung Song; Mihue Jang; Soo Youn Kim; Goeun Lee; Gil-Je Lee; Dae Heon Kim; Yongjik Lee; Wonhwa Cho; Inhwan Hwang
Source:: Plant physiology 2012 v.159 no.3 pp. 1013-1025
ISSN:: 0032-0889
Subject:: Arabidopsis thaliana; cell walls; clathrin; cytokinesis; endosomes; mitosis; plasma membrane
Abstract:: ... Cytokinesis is the process of partitioning the cytoplasm of a dividing cell, thereby completing mitosis. Cytokinesis in the plant cell is achieved by the formation of a new cell wall between daughter nuclei using components carried in Golgi-derived vesicles that accumulate at the midplane of the phragmoplast and fuse to form the cell plate. Proteins that play major roles in the development of the ...
DOI:: 10.1104/pp.112.199380
PubMed:: 22635117
PubMed Central:: PMC3387690
: https://doi.org/10.1104/pp.112.199380

50. AAA ATPase p97/VCP is essential for TRIM21-mediated virus neutralization

Author:: Felix Hauler; Donna L. Mallery; William A. McEwan; Susanna R. Bidgood; Leo C. James
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.48 pp. 19733-19738
ISSN:: 0027-8424
Subject:: adenosinetriphosphatase; antibodies; capsid; immunity; immunoglobulin G; neutralization; neutralization tests; proteasome endopeptidase complex; virion; viruses
Abstract:: ... Tripartite motif-containing 21 (TRIM21) is a cytosolic IgG receptor that mediates intracellular virus neutralization by antibody. TRIM21 targets virions for destruction in the proteasome, but it is unclear how a substrate as large as a viral capsid is degraded. Here, we identify the ATPase p97/valosin-containing protein (VCP), an enzyme with segregase and unfoldase activity, as a key player in thi ...
DOI:: 10.1073/pnas.1210659109
PubMed:: 23091005
PubMed Central:: PMC3511720
: https://doi.org/10.1073/pnas.1210659109

51. ABA signal in rice under stress conditions

Author:: Nenghui Ye; Liguo Jia; Jianhua Zhang
Source:: Rice 2012 v.5 no.1 pp. 1
ISSN:: 1939-8425
Subject:: abscisic acid; biosynthesis; plant development; plant pathogens; receptors; rice; stress response; transporters
Abstract:: ... Ever since its discovery, abscisic acid (ABA) has been intensively studied due to its versatile functions in plant developmental and physiological processes. Many signaling details of ABA have been well elucidated and reviewed. The identification of ABA receptors is a great breakthrough in the field of ABA study, whereas the discovery of ABA transporter has changed our concept that ABA is delivere ...
DOI:: 10.1186/1939-8433-5-1
PubMed:: 24764501
PubMed Central:: PMC3834477
: https://doi.org/10.1186/1939-8433-5-1

52. The ABCs of molecular dynamics simulations on B-DNA, circa 2012

Author:: David L Beveridge; Thomas E Cheatham III; Mihaly Mezei
Source:: Journal of biosciences 2012 v.37 no.3 pp. 379-397
ISSN:: 0250-5991
Subject:: B-DNA; databases; genes; ions; models; molecular dynamics; nuclear magnetic resonance spectroscopy; nucleotide sequences
Abstract:: ... This article provides a retrospective on the ABC initiative in the area of all-atom molecular dynamics (MD) simulations including explicit solvent on all tetranucleotide steps of duplex B-form DNA duplex, ca. 2012. The ABC consortium has completed two phases of simulations, the most current being a set of 50â100 trajectories based on the AMBER ff99 force field together with the parmbsc0 modifica ...
DOI:: 10.1007/s12038-012-9222-6
PubMed:: 22750978
PubMed Central:: PMC4029509
: https://doi.org/10.1007/s12038-012-9222-6

53. ACCELERATED CELL DEATH 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis

Author:: Gopal K. Pattanayak; Sujatha Venkataramani; Stefan Hortensteiner; Lukas Kunz; Bastien Christ; Michael Moulin; Alison G. Smith; Yukihiro Okamoto; Hitoshi Tamiaki; Masakazu Sugishima; Jean T. Greenberg
Source:: plant journal 2012 v.69 no.4 pp. 589-600
ISSN:: 0960-7412
Subject:: Arabidopsis; apoptosis; bacterial infections; chlorophyll; chloroplasts; enzymes; leaves; mitochondria; oxygen; phenotype; proteins; protoporphyrin
Abstract:: ... The Arabidopsis ACCELERATED CELL DEATH 2 (ACD2) protein protects cells from programmed cell death (PCD) caused by endogenous porphyrin‐related molecules like red chlorophyll catabolite or exogenous protoporphyrin IX. We previously found that during bacterial infection, ACD2, a chlorophyll breakdown enzyme, localizes to both chloroplasts and mitochondria in leaves. Additionally, acd2 cells show mit ...
DOI:: 10.1111/j.1365-313X.2011.04814.x
PubMed:: 21988537
PubMed Central:: PMC3274588
: https://doi.org/10.1111/j.1365-313X.2011.04814.x

54. The ADAR protein family

Author:: Yiannis A Savva; Leila E Rieder; Robert A Reenan
Source:: Genome biology 2012 v.13 no.12 pp. 2975
ISSN:: 1474-760X
Subject:: RNA editing; RNA interference; adenosine; adenosine deaminase; double-stranded RNA; gene expression; genes; ion channels; mutation; nervous system; peptides; phenotype
Abstract:: ... Adenosine to inosine (A-to-I) RNA editing is a post-transcriptional process by which adenosines are selectively converted to inosines in double-stranded RNA (dsRNA) substrates. A highly conserved group of enzymes, the adenosine deaminase acting on RNA (ADAR) family, mediates this reaction. All ADARs share a common domain architecture consisting of a variable number of amino-terminal dsRNA binding ...
DOI:: 10.1186/gb-2012-13-12-252
PubMed:: 23273215
PubMed Central:: PMC3580408
: https://doi.org/10.1186/gb-2012-13-12-252

55. AGL24 acts in concert with SOC1 and FUL during Arabidopsis floral transition

Author:: Stefano Torti; Fabio Fornara
Source:: Plant signaling & behavior 2012 v.7 no.10 pp. 1251-1254
ISSN:: 1559-2324
Subject:: Arabidopsis; apical meristems; flowering; flowers; leaves; messenger RNA; mutants; mutation
Abstract:: ... Arabidopsis plants flower in response to long days (LDs). Exposure of leaves to inductive day lengths activates expression of FLOWERING LOCUS T (FT) protein which moves to the shoot apical meristem (SAM) to induce developmental reprogramming. SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) and FRUITFULL (FUL) are induced by FT at the apex. We previously screened the SAM for mRNAs of genes requir ...
DOI:: 10.4161/psb.21552
PubMed:: 22902690
PubMed Central:: PMC3493406
: https://doi.org/10.4161/psb.21552

56. AGNPS GIS-Based Tool for Watershed-Scale Identification and Mapping of Cropland Potential Ephemeral Gullies

Author:: Momm, H.G.; Bingner, R.L.; Wells, R.R.; Wilcox, D.
Source:: Applied engineering in agriculture 2012 v.28 no.1 pp. 17-29
ISSN:: 1943-7838
Subject:: AGNPS model; agricultural land; case studies; conservation practices; geographic information systems; gully erosion; pollution; sediment yield; sediments; user interface; watersheds
Abstract:: ... AnnAGNPS, Compound topographic index, Ephemeral gully, GIS, Cropland erosion. The formation of ephemeral gullies in agricultural fields has been recognized as an important source of sediment contributing to environmental degradation and compromising crop productivity. Methodologies are being developed for assessing gully formation and gully sediment yield. The Annualized Agricultural Non-Point Sou ...
Handle:: 10113/54162
DOI:: 10.13031/2013.41282
: https://doi.org/10.13031/2013.41282

57. The AINTEGUMENTA LIKE1 Homeotic Transcription Factor PtAIL1 Controls the Formation of Adventitious Root Primordia in Poplar

Author:: Adeline Rigal; Yordan S. Yordanov; Irene Perrone; Anna Karlberg; Emilie Tisserant; Catherine Bellini; Victor B. Busov; Francis Martin; Annegret Kohler; Rishi Bhalerao; Valérie Legué
Source:: Plant physiology 2012 v.160 no.4 pp. 1996-2006
ISSN:: 0032-0889
Subject:: germplasm; transcription factors; genetically modified organisms; RNA interference; gene overexpression; microarray technology; early development; adventitious roots; Populus trichocarpa; mature plants; rooting; genes; root primordia; transcription (genetics); transcriptome; elite trees
Abstract:: ... Adventitious rooting is an essential but sometimes rate-limiting step in the clonal multiplication of elite tree germplasm, because the ability to form roots declines rapidly with age in mature adult plant tissues. In spite of the importance of adventitious rooting, the mechanism behind this developmental process remains poorly understood. We have described the transcriptional profiles that are as ...
DOI:: 10.1104/pp.112.204453
PubMed:: 23077242
PubMed Central:: PMC3510126
: https://doi.org/10.1104/pp.112.204453

58. AMP-activated protein kinase stimulates myostatin expression in C2C12 cells

Author:: Arun K. Das; Qi-Yuan Yang; Xing Fu; Jun-Fang Liang; Marcio S. Duarte; Mei-Jun Zhu; Grant D. Trobridge; Min Du
Source:: Biochemical and biophysical research communications 2012 v.427 no.1 pp. 36-40
ISSN:: 0006-291X
Subject:: AMP-activated protein kinase; energy metabolism; gene expression; mammals; messenger RNA; metformin; models; muscle protein; muscles; mutants; myoblasts; myostatin; protein content; protein degradation; protein synthesis; skeletal muscle
Abstract:: ... AMP-activated protein kinase (AMPK) is a master regulator of energy metabolism in skeletal muscle; AMPK induces muscle protein degradation but the underlying mechanisms are unclear. Myostatin is a powerful negative regulator of skeletal muscle mass and growth in mammalian species. We hypothesized that AMPK stimulates myostatin expression, which provides an explanation for the negative role of AMPK ...
DOI:: 10.1016/j.bbrc.2012.08.138
PubMed:: 22995402
PubMed Central:: PMC3486733
: https://doi.org/10.1016/j.bbrc.2012.08.138

59. The APC/C Subunit Mnd2/Apc15 Promotes Cdc20 Autoubiquitination and Spindle Assembly Checkpoint Inactivation

Author:: Scott A. Foster; David O. Morgan
Source:: Molecular cell 2012 v.47 no.6 pp. 921-932
ISSN:: 1097-2765
Subject:: anaphase; chromosome segregation; kinetochores; ubiquitination; yeasts
Abstract:: ... The fidelity of chromosome segregation depends on the spindle assembly checkpoint (SAC). In the presence of unattached kinetochores, anaphase is delayed when three SAC components (Mad2, Mad3/BubR1, and Bub3) inhibit Cdc20, the activating subunit of the anaphase-promoting complex (APC/C). We analyzed the role of Cdc20 autoubiquitination in the SAC of budding yeast. Reconstitution with purified comp ...
DOI:: 10.1016/j.molcel.2012.07.031
PubMed:: 22940250
PubMed Central:: PMC3462283
: https://doi.org/10.1016/j.molcel.2012.07.031

60. APOA5 genotype modulates 2-y changes in lipid profile in response to weight-loss diet intervention: the Pounds Lost Trial

Author:: Xiaomin Zhang; Qibin Qi; George A Bray; Frank B Hu; Frank M Sacks; Lu Qi
Source:: American journal of clinical nutrition 2012 v.96 no.4 pp. 917-922
ISSN:: 0002-9165
Subject:: adults; alleles; blood serum; clinical nutrition; data collection; energy; fasting; fat intake; genotype; high density lipoprotein cholesterol; high fat diet; lipid metabolism; low density lipoprotein cholesterol; low fat diet; major genes; nutritional intervention; overweight; risk; triacylglycerols; weight loss
Abstract:: ... Background: The apolipoprotein A5 gene (APOA5) is a major gene that regulates lipid metabolism and is modulated by dietary factors. A novel variant rs964184 in APOA5 was identified to be associated with lipids in genome-wide association studies.Objective: We examined whether this variant modified changes in lipid concentrations in response to a 2-y weight-loss diet intervention in a randomized tri ...
DOI:: 10.3945/ajcn.112.040907
PubMed:: 22914552
PubMed Central:: PMC3441115
: https://doi.org/10.3945/ajcn.112.040907

61. APOBEC2 Is a Monomer in Solution: Implications for APOBEC3G Models

Author:: Krzysiak Troy C.; Jung Jinwon; Thompson James; Baker David; Gronenborn Angela M.
Source:: Biochemistry 2012 v.51 no.9 pp. 2008-2017
ISSN:: 1520-4995
Subject:: Human immunodeficiency virus; antiretroviral properties; crystal structure; crystallization; models; nuclear magnetic resonance spectroscopy; pathogenicity; prediction
Abstract:: ... Although the physiological role of APOBEC2 is still largely unknown, a crystal structure of a truncated variant of this protein was determined several years ago [Prochnow, C. (2007) Nature445, 447–451]. This APOBEC2 structure had considerable impact in the HIV field because it was considered a good model for the structure of APOBEC3G, an important HIV restriction factor that abrogates HIV infectiv ...
DOI:: 10.1021/bi300021s
PubMed:: 22339232
PubMed Central:: PMC3316327
: https://doi.org/10.1021/bi300021s

62. The AR dependent cell cycle: Mechanisms and cancer relevance

Author:: Matthew J. Schiewer; Michael A. Augello; Karen E. Knudsen
Source:: Molecular and cellular endocrinology 2012 v.352 no.1-2 pp. 34-45
ISSN:: 0303-7207
Subject:: androgen receptors; cell cycle; cell proliferation; human diseases; neoplasm cells; prostatic neoplasms
Abstract:: ... Prostate cancer cells are exquisitely dependent on androgen receptor (AR) activity for proliferation and survival. As these functions are critical targets of therapeutic intervention for human disease, it is imperative to delineate the mechanisms by which AR engages the cell cycle engine. More than a decade of research has revealed that elegant intercommunication between AR and the cell cycle mach ...
DOI:: 10.1016/j.mce.2011.06.033
PubMed:: 21782001
PubMed Central:: PMC3641823
: https://doi.org/10.1016/j.mce.2011.06.033

63. ARABIDOPSIS TRITHORAX-RELATED3/SET DOMAIN GROUP2 is Required for the Winter-Annual Habit of Arabidopsis thaliana

Author:: Jae-Young Yun; Yosuke Tamada; Ye Eun Kang; Richard M. Amasino
Source:: Plant & cell physiology 2012 v.53 no.5 pp. 834-846
ISSN:: 0032-0781
Subject:: Arabidopsis thaliana; Drosophila; alleles; chromatin; flowering; loci; lysine; methyltransferases; mutants; yeasts
Abstract:: ... The winter-annual habit of Arabidopsis thaliana requires active alleles of FLOWERING LOCUS C (FLC), which encodes a potent flowering repressor, and FRIGIDA (FRI), an activator of FLC. FLC activation by FRI is accompanied by an increase in specific histone modifications, such as tri-methylation of histone H3 at lysine 4 (H3K4me3), and requires three H3K4 methyltransferases, the Drosophila Trithorax ...
DOI:: 10.1093/pcp/pcs021
PubMed:: 22378382
PubMed Central:: PMC3345368
: https://doi.org/10.1093/pcp/pcs021

64. ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting

Author:: Melissa C. Humbert; Katie Weihbrecht; Charles C. Searby; Yalan Li; Robert M. Pope; Val C. Sheffield; Seongjin Seo
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.48 pp. 19691-19696
ISSN:: 0027-8424
Subject:: cilia; genetic disorders; genetic techniques and protocols; humans; inositols; missense mutation; protein-protein interactions; proteins
Abstract:: ... Mutations affecting ciliary components cause a series of related genetic disorders in humans, including nephronophthisis (NPHP), Joubert syndrome (JBTS), Meckel-Gruber syndrome (MKS), and Bardet-Biedl syndrome (BBS), which are collectively termed “ciliopathies.” Recent protein–protein interaction studies combined with genetic analyses revealed that ciliopathy-related proteins form several function ...
DOI:: 10.1073/pnas.1210916109
PubMed:: 23150559
PubMed Central:: PMC3511769
: https://doi.org/10.1073/pnas.1210916109

65. The ARP2/3 Complex Mediates Guard Cell Actin Reorganization and Stomatal Movement in Arabidopsis

Author:: Kun Jiang; Karim Sorefan; Michael J. Deeks; Michael W. Bevan; Patrick J. Hussey; Alistair M. Hetherington
Source:: plant cell 2012 v.24 no.5 pp. 2031-2040
ISSN:: 1040-4651
Subject:: Arabidopsis; actin; alleles; guard cells; loci; microfilaments; stomatal movement
Abstract:: ... This study reports the isolation of hsr3 , an allele of the Arabidopsis ARPC2 locus encoding a subunit of the ARP2/3 complex involved in the regulation of the actin cytoskeleton. In hsr3 , reduced abscisic acid–induced stomatal closure is associated with failure of actin reassembly, indicating that modulation of the cytoskeleton through the ARP2/3 complex is crucial for stomatal regulation. ...
DOI:: 10.1105/tpc.112.096263
PubMed:: 22570440
PubMed Central:: PMC3442585
: https://doi.org/10.1105/tpc.112.096263

66. ATH1 and KNAT2 proteins act together in regulation of plant inflorescence architecture

Author:: Yang Li; Limin Pi; Hai Huang; Lin Xu
Source:: Journal of experimental botany 2012 v.63 no.3 pp. 1423-1433
ISSN:: 0022-0957
Subject:: Arabidopsis thaliana; fluorescence; genes; genetic techniques and protocols; inflorescences; mutants; pedicel; plant architecture; plant reproduction; proteins
Abstract:: ... The inflorescence of flowering plants is a highly organized structure, not only contributing to plant reproductive processes, but also constituting an important part of the entire plant morphology. Previous studies have revealed that the class-I KNOTTED1-like homeobox (KNOX) genes BREVIPEDICELLUS (BP or KNAT1), KNAT2, and KNAT6 play essential roles in inflorescence architecture. Pedicel morphology ...
DOI:: 10.1093/jxb/err376
PubMed:: 22140242
PubMed Central:: PMC3276100
: https://doi.org/10.1093/jxb/err376

67. ATHB4 and HAT3, two class II HD-ZIP transcription factors, control leaf development in Arabidopsis

Author:: Jordi Bou-Torrent; Mercè Salla-Martret; Ronny Brandt; Thomas Musielak; Jean-Christophe Palauqui; Jaime F. Martínez-García; Stephan Wenkel
Source:: Plant signaling & behavior 2012 v.7 no.11 pp. 1382-1387
ISSN:: 1559-2324
Subject:: Arabidopsis; auxins; biosynthesis; canopy; cotyledons; genes; growth promotion; leaf development; leaves; loss-of-function mutation; transcription factors
Abstract:: ... In response to plant proximity or canopy shade, plants can react by altering elongation growth and development. Several members of the class II homeodomain-leucine zipper (HD-ZIPII) transcription factor family have been shown to play an instrumental role in the responses to shade. HD-ZIP members of the class III (HD-ZIPIII), by contrast, are involved in basic patterning processes. We recently show ...
DOI:: 10.4161/psb.21824
PubMed:: 22918502
PubMed Central:: PMC3548853
: https://doi.org/10.4161/psb.21824

68. ATI1, a newly identified atg8-interacting protein, binds two different Atg8 homologs

Author:: Tamar Avin-Wittenberg; Simon Michaeli; Arik Honig; Gad Galili
Source:: Plant signaling & behavior 2012 v.7 no.6 pp. 685-687
ISSN:: 1559-2324
Subject:: Arabidopsis thaliana; abscisic acid; autophagy; germination; proteins; seeds; vacuoles
Abstract:: ... Autophagy is a mechanism used for the transport of macromolecules to the vacuole for degradation. It can be either non-selective or selective, resulting from the specific binding of target proteins to Atg8, an essential autophagy-related protein. Nine Atg8 homologs exist in the model plant Arabidopsis thaliana, suggesting possible different roles for different homologs. In a previous report publis ...
DOI:: 10.4161/psb.20030
PubMed:: 22580699
PubMed Central:: PMC3442868
: https://doi.org/10.4161/psb.20030

69. ATM kinase inhibition in glial cells activates the innate immune response and causes neurodegeneration in Drosophila

Author:: Andrew J. Petersen; Stacey A. Rimkus; David A. Wassarman
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.11 pp. E656
ISSN:: 0027-8424
Subject:: DNA repair; Drosophila; adults; apoptosis; brain; cell cycle; genes; immunity; inflammation; longevity; mutants; mutation; neurodegenerative diseases; neuroglia; neurons; protein kinases
Abstract:: ... To investigate the mechanistic basis for central nervous system (CNS) neurodegeneration in the disease ataxia–telangiectasia (A-T), we analyzed flies mutant for the causative gene A-T mutated (ATM). ATM encodes a protein kinase that functions to monitor the genomic integrity of cells and control cell cycle, DNA repair, and apoptosis programs. Mutation of the C-terminal amino acid in Drosophila ATM ...
DOI:: 10.1073/pnas.1110470109
PubMed:: 22355133
PubMed Central:: PMC3306708
: https://doi.org/10.1073/pnas.1110470109

70. ATM kinase is activated by sindbis viral vector infection

Author:: Christine Pampeno; Alicia Hurtado; Daniel Meruelo
Source:: Virus research 2012 v.166 no.1-2 pp. 97-102
ISSN:: 0168-1702
Subject:: DNA replication; Sindbis virus; cytotoxicity; gene therapy; genotoxicity; histones; neoplasms; protein synthesis; viruses
Abstract:: ... Sindbis virus is a prototypic member of the Alphavirus genus, Togaviridae family. Sindbis replication results in cellular cytotoxicity, a feature that has been exploited by our laboratory for treatment of in vivo tumors. Understanding the interactions between Sindbis vectors and the host cell can lead to better virus production and increased efficacy of gene therapy vectors. Here we present studie ...
DOI:: 10.1016/j.virusres.2012.03.008
PubMed:: 22475743
PubMed Central:: PMC3351575
: https://doi.org/10.1016/j.virusres.2012.03.008

71. ATP Binding and Hydrolysis-Driven Rate-Determining Events in the RFC-Catalyzed PCNA Clamp Loading Reaction

Author:: Miho Sakato; Yayan Zhou; Manju M. Hingorani
Source:: Journal of molecular biology 2012 v.416 no.2 pp. 176-191
ISSN:: 0022-2836
Subject:: Saccharomyces cerevisiae; adenosine triphosphate; hydrolysis; models; plastid DNA; proliferating cell nuclear antigen
Abstract:: ... The multi-subunit replication factor C (RFC) complex loads circular proliferating cell nuclear antigen (PCNA) clamps onto DNA where they serve as mobile tethers for polymerases and coordinate the functions of many other DNA metabolic proteins. The clamp loading reaction is complex, involving multiple components (RFC, PCNA, DNA, and ATP) and events (minimally: PCNA opening/closing, DNA binding/rele ...
DOI:: 10.1016/j.jmb.2011.12.018
PubMed:: 22197378
PubMed Central:: PMC3437054
: https://doi.org/10.1016/j.jmb.2011.12.018

72. ATP activates P2X receptors to mediate gap junctional coupling in the cochlea

Author:: Yan Zhu; Hong-Bo Zhao
Source:: Biochemical and biophysical research communications 2012 v.426 no.4 pp. 528-532
ISSN:: 0006-291X
Subject:: adenosine triphosphate; agonists; antagonists; calcium; gap junctions; hearing; homeostasis; receptors; uridine triphosphate
Abstract:: ... ATP is an important extracellular signaling molecule and can activate both ionotropic (P2X) and metabotropic purinergic (P2Y) receptors to influence cellular function in many aspects. Gap junction is an intercellular channel and plays a critical role in hearing. Here, we report that stimulation of ATP reduced gap junctional coupling between cochlear supporting cells. This uncoupling effect could b ...
DOI:: 10.1016/j.bbrc.2012.08.119
PubMed:: 22982314
PubMed Central:: PMC3471361
: https://doi.org/10.1016/j.bbrc.2012.08.119

73. ATP-Binding Cassette B4, an Auxin-Efflux Transporter, Stably Associates with the Plasma Membrane and Shows Distinctive Intracellular Trafficking from That of PIN-FORMED Proteins

Author:: Misuk Cho; Zee-Won Lee; Hyung-Taeg Cho
Source:: Plant physiology 2012 v.159 no.2 pp. 642-654
ISSN:: 0032-0889
Subject:: Arabidopsis thaliana; auxins; endocytosis; endosomes; fluorescence; homeostasis; photobleaching; plant development; plasma membrane; roots; transporters
Abstract:: ... Intracellular trafficking of auxin transporters has been implicated in diverse developmental processes in plants. Although the dynamic trafficking pathways of PIN-FORMED auxin efflux proteins have been studied intensively, the trafficking of ATP-binding cassette protein subfamily B proteins (ABCBs; another group of auxin efflux carriers) still remains largely uncharacterized. In this study, we add ...
DOI:: 10.1104/pp.112.196139
PubMed:: 22492845
PubMed Central:: PMC3375931
: https://doi.org/10.1104/pp.112.196139

74. ATP-Triggered Conformational Changes Delineate Substrate-Binding and -Folding Mechanics of the GroEL Chaperonin

Author:: Daniel K. Clare; Daven Vasishtan; Scott Stagg; Joel Quispe; George W. Farr; Maya Topf; Arthur L. Horwich; Helen R. Saibil
Source:: Cell 2012 v.149 no.1 pp. 113-123
ISSN:: 0092-8674
Subject:: adenosine triphosphate; binding sites; cryo-electron microscopy; encapsulation; hydrophilicity; hydrophobicity; mechanics; polypeptides; statistical analysis
Abstract:: ... The chaperonin GroEL assists the folding of nascent or stress-denatured polypeptides by actions of binding and encapsulation. ATP binding initiates a series of conformational changes triggering the association of the cochaperonin GroES, followed by further large movements that eject the substrate polypeptide from hydrophobic binding sites into a GroES-capped, hydrophilic folding chamber. We used c ...
DOI:: 10.1016/j.cell.2012.02.047
PubMed:: 22445172
PubMed Central:: PMC3326522
: https://doi.org/10.1016/j.cell.2012.02.047

75. ATP-binding cassette transporter A1 and HDL metabolism: effects of fatty acids

Author:: Jiyoung Lee; Youngki Park; Sung I. Koo
Source:: Journal of nutritional biochemistry 2012 v.23 no.1 pp. 1-7
ISSN:: 0955-2863
Subject:: metabolism; liver; protein kinases; histones; unsaturated fatty acids; high density lipoprotein cholesterol; transcription (genetics); saturated fatty acids; apolipoprotein A-I; phosphorylation; protein degradation; ABC transporters; phospholipids
Abstract:: ... Ample evidence indicates that dietary fatty acids alter the plasma levels of high-density lipoprotein cholesterol (HDL-C). However, the mechanisms underlying the effects of fatty acids still remain elusive. Recent advances in our understanding of ATP-binding cassette transporter A1 (ABCA1) function and regulation have provided a valuable insight into the mechanisms by which fatty acids may affect ...
DOI:: 10.1016/j.jnutbio.2011.03.001
PubMed:: 21684139
PubMed Central:: PMC3204154
: https://doi.org/10.1016/j.jnutbio.2011.03.001

76. ATP-independent substrate reduction by nitrogenase P-cluster variant

Author:: Chi Chung Lee; Yilin Hu; Markus W. Ribbe
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.18 pp. 6922-6926
ISSN:: 0027-8424
Subject:: acetylene; alkanes; biomimetics; carbon dioxide; carbon monoxide; catalysts; catalytic activity; cyanides; electron transfer; ethylene; europium; hydrazine; nitrogenase; proteins
Abstract:: ... The P-cluster of nitrogenase is largely known for its function to mediate electron transfer to the active cofactor site during catalysis. Here, we show that a P-cluster variant (designated P*-cluster), which consists of paired [Fe4S4]-like clusters, can catalyze ATP-independent substrate reduction in the presence of a strong reductant, europium(II) diethylenetriaminepentaacetate [Eu(II)-DTPA]. The ...
DOI:: 10.1073/pnas.1202429109
PubMed:: 22509042
PubMed Central:: PMC3344972
: https://doi.org/10.1073/pnas.1202429109

77. AU-rich element-binding protein negatively regulates CCAAT enhancer-binding protein mRNA stability during long-term synaptic plasticity in Aplysia

Author:: Yong-Seok Lee; Sun-Lim Choi; Heejung Jun; Se-Jeong Yim; Jin-A Lee; Hyoung F. Kim; Seung-Hee Lee; Jaehoon Shim; Kyungmin Lee; Deok-Jin Jang; Bong-Kiun Kaang
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.38 pp. 15520-15525
ISSN:: 0027-8424
Subject:: gene overexpression; memory; messenger RNA; reporter genes; sensory neurons; serotonin
Abstract:: ... The consolidation of long-term memory for sensitization and synaptic facilitation in Aplysia requires synthesis of new mRNA including the immediate early gene Aplysia CCAAT enhancer-binding protein (ApC/EBP). After the rapid induction of ApC/EBP expression in response to repeated treatments of 5-hydroxytryptamine (5-HT), ApC/EBP mRNA is temporarily expressed in sensory neurons of sensory-to-motor ...
DOI:: 10.1073/pnas.1116224109
PubMed:: 22949683
PubMed Central:: PMC3458389
: https://doi.org/10.1073/pnas.1116224109

78. AUX/LAX Genes Encode a Family of Auxin Influx Transporters That Perform Distinct Functions during Arabidopsis Development

Author:: Benjamin Péret; Kamal Swarup; Alison Ferguson; Malvika Seth; Yaodong Yang; Stijn Dhondt; Nicholas James; Ilda Casimiro; Paula Perry; Adnan Syed; Haibing Yang; Jesica Reemmer; Edward Venison; Caroline Howells; Miguel A. Perez-Amador; Jeonga Yun; Jose Alonso; Gerrit T.S. Beemster; Laurent Laplaze; Angus Murphy; Malcolm J. Bennett; Erik Nielsen; Ranjan Swarup
Source:: plant cell 2012 v.24 no.7 pp. 2874-2885
ISSN:: 1040-4651
Subject:: Arabidopsis; auxins; cotyledons; genes; plant development; transporters
Abstract:: ... This article describes the role of AUX/LAX auxin influx carriers in plant development, revealing that the auxin influx carrier LAX2 regulates vascular patterning in cotyledons. Although the AUX1/LAX family members share auxin transport characteristics, these transport activities seem to be dependent on their unique cell- or tissue-type expression patterns. ...
DOI:: 10.1105/tpc.112.097766
PubMed:: 22773749
PubMed Central:: PMC3426120
: https://doi.org/10.1105/tpc.112.097766

79. AWSEM-MD: Protein Structure Prediction Using Coarse-Grained Physical Potentials and Bioinformatically Based Local Structure Biasing

Author:: Davtyan Aram; Schafer Nicholas P.; Zheng Weihua; Clementi Cecilia; Wolynes Peter G.; Papoian Garegin A.
Source:: Journal of physical chemistry 2012 v.116 no.29 pp. 8494-8503
ISSN:: 1520-5207
Subject:: databases; energy; hydrogen bonding; models; prediction; protein structure; proteins; sequence alignment; sequence homology
Abstract:: ... The associative memory, water mediated, structure and energy model (AWSEM) is a coarse-grained protein force field. AWSEM contains physically motivated terms, such as hydrogen bonding, as well as a bioinformatically based local structure biasing term, which efficiently takes into account many-body effects that are modulated by the local sequence. When combined with appropriate local or global alig ...
DOI:: 10.1021/jp212541y
PubMed:: 22545654
PubMed Central:: PMC3406225
: https://doi.org/10.1021/jp212541y

80. Abdominal segment reduction:Development and evolution of a deeply fixed trait

Author:: John H. Yoder
Source:: Fly 2012 v.6 no.4 pp. 240-245
ISSN:: 1933-6942
Subject:: Drosophila melanogaster; adults; dynamics; epithelium; evolution; females; genes; homeodomain proteins; males; models; morphogenesis; pigmentation; pupation; sexual dimorphism; sorting; transcription factors
Abstract:: ... When a new student first begins to push flies, an immediate skill that must be learned is sorting the sexes. In Drosophila melanogaster several sexually dimorphic characters can be used to readily distinguish males from females including abdominal pigmentation, male sex combs and genital morphology. Another, often-overlooked, sexual dimorphism is adult abdominal segment number. Externally, adult D ...
DOI:: 10.4161/fly.22109
PubMed:: 23026836
PubMed Central:: PMC3519658
: https://doi.org/10.4161/fly.22109

81. Aberrant S-nitrosylation mediates calcium-triggered ventricular arrhythmia in the intact heart

Author:: Michael J. Cutler; Bradley N. Plummer; Xiaoping Wan; Qi-An Sun; Douglas Hess; Haiyan Liu; Isabelle Deschenes; David S. Rosenbaum; Jonathan S. Stamler; Kenneth R. Laurita
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.44 pp. 18186-18191
ISSN:: 0027-8424
Subject:: allopurinol; arrhythmia; calcium; death; heart; myocytes; neuronal nitric oxide synthase; nitric oxide; oxidation; oxidative stress; phenotype; phosphorylation; physiological state; proteins; reactive oxygen species; sarcoplasmic reticulum; xanthine
Abstract:: ... Nitric oxide (NO) derived from the activity of neuronal nitric oxide synthase (NOS1) is involved in S-nitrosylation of key sarcoplasmic reticulum (SR) Ca ²⁺ handling proteins. Deficient S-nitrosylation of the cardiac ryanodine receptor (RyR2) has a variable effect on SR Ca ²⁺ leak/sparks in isolated myocytes, likely dependent on the underlying physiological state. It remains unknown, however, whet ...
DOI:: 10.1073/pnas.1210565109
PubMed:: 23071315
PubMed Central:: PMC3497770
: https://doi.org/10.1073/pnas.1210565109

82. Aberrant expression of c-Jun in glioblastoma by internal ribosome entry site (IRES)-mediated translational activation

Author:: Lior Blau; Revital Knirsh; Iris Ben-Dror; Sivan Oren; Silke Kuphal; Peter Hau; Martin Proescholdt; Anja-Katrin Bosserhoff; Lily Vardimon
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.42 pp. E2875
ISSN:: 0027-8424
Subject:: 5' untranslated regions; DNA microarrays; cell death; cell proliferation; half life; humans; mitogen-activated protein kinase; neoplasms; ribosomes; therapeutics; translation (genetics)
Abstract:: ... Although the protooncogene c-Jun plays a critical role in cell proliferation, cell death, and malignant transformation, DNA microarray screens have identified only a few human cancer types with aberrant expression of c-Jun. Here, we show that c-Jun accumulation is robustly elevated in human glioblastoma and that this increase contributes to the malignant properties of the cells. Most importantly, ...
DOI:: 10.1073/pnas.1203659109
PubMed:: 23027969
PubMed Central:: PMC3479486
: https://doi.org/10.1073/pnas.1203659109

83. Aberrant microtubule organization in dividing root cells of p60-katanin mutants

Author:: Emmanuel Panteris; Ioannis-Dimosthenis S. Adamakis
Source:: Plant signaling & behavior 2012 v.7 no.1 pp. 16-18
ISSN:: 1559-2324
Subject:: Arabidopsis thaliana; anaphase; metaphase; microtubules; mutants; nuclear membrane; phenotype; prophase
Abstract:: ... Aberrant microtubule organization has been recently recorded in dividing root cells of fra2 and lue1 p60-katanin Arabidopsis thaliana mutants. Here, we report similar defects in the bot1 and ktn1-2 mutants of the same plant, proposing that they constitute a consistent phenotype of p60-katanin mutants. In addition, we show that the Targeting Protein for Xklp2 (TPX2) protein co-localizes with microt ...
DOI:: 10.4161/psb.7.1.18358
PubMed:: 22301959
PubMed Central:: PMC3357358
: https://doi.org/10.4161/psb.7.1.18358

84. Ability of MEQ-Deleted MDV Vaccine Candidates to Adversely Affect Lymphoid Organs and Chicken Weight Gain.

Author:: John R. Dunn; Robert F. Silva
Source:: Avian diseases 2012 v.56 no. pp. 494-500
ISSN:: 0005-2086
Subject:: Mardivirus; Marek disease; adverse effects; atrophy; bursa of Fabricius; chickens; thymus gland; vaccination; vaccines; virus replication; weight gain
Abstract:: ... CVI988 (Rispens) is currently the most effective vaccine used to protect against Marek’s disease, a lymphoproliferative disease of chickens. A MEQ-deleted Marek’s disease virus strain has shown promise as a vaccine candidate; however, unpublished results from vaccine safety trials suggest that this candidate vaccine induces unwanted lymphoid atrophy. The current study evaluated lymphoid atrophy at ...
Handle:: 10113/57590

85. Ability of rumen protozoa Diploplastron affine to utilize Î²-glucans

Author:: Grzegorz Belzecki; Renata Miltko; Tadeusz Michalowski
Source:: Folia microbiologica 2012 v.57 no.4 pp. 259-262
ISSN:: 0015-5632
Subject:: Ciliophora; antibiotics; beta-glucans; cellulose; culture media; curdlan; digestion; enrichment culture; glucose; pH; population density; reducing sugars; rumen; rumen protozoa; sheep; xylan
Abstract:: ... The ability of the rumen ciliates to utilize Î²-glucans other than cellulose and xylan is currently being recognized. The objective of the present study was to characterize the ability of the ciliate Diploplastron affine to digest some pachyman, laminarin, pustulan, curdlan and lichean. The protozoa were isolated from the rumen of sheep and either grown in vitro or inoculated into the rumen of cil ...
DOI:: 10.1007/s12223-012-0120-y
PubMed:: 22528300
PubMed Central:: PMC3389252
: https://doi.org/10.1007/s12223-012-0120-y

86. Abiotic Factors Affecting Canola Establishment and Insect Pest Dynamics

Author:: Nansen Christian; Trostle Calvin; Angadi Sangu; Porter Patrick; Martini Xavier
Source:: International journal of agronomy 2012 v.2012 pp. -
ISSN:: 1687-8159
Subject:: Aphidoidea; biodiesel; canola; environmental factors; insect pests; oilseed crops; plant establishment; relative humidity; seed treatment; seeds; solar radiation; temperature; wind speed; Texas
Abstract:: ... Canola is grown mainly as an oil-seed crop, but recently the interest in canola has increased due to its potential as a biodiesel crop. The main objectives of this paper were to evaluate effects of abiotic factors and seed treatment on canola plant establishment and pest pressure in the Southern High Plains of Texas. Data was collected at two field locations during the first seven months of two fi ...
DOI:: 10.1155/2012/838903
CHORUS:: 10.1155/2012/838903
Chorus Open Access:: 10.1155/2012/838903
: https://doi.org/10.1155/2012/838903

87. Abiotic and biotic factors associated with the presence of Anopheles arabiensis immatures and their abundance in naturally occurring and man-made aquatic habitats

Author:: Louis Clément Gouagna; Manpionona Rakotondranary; Sebastien Boyer; Guy Lempérière; Jean-Sébastien Dehecq; Didier Fontenille
Source:: Parasites & vectors 2012 v.5 no.1 pp. 96
ISSN:: 1756-3305
Subject:: Anopheles arabiensis; altitude; aquatic habitat; breeding sites; control methods; fauna; immatures; larvae; larval development; malaria; physicochemical properties; predation; species diversity; surveys; vector control; Africa; Reunion
Abstract:: ... BACKGROUND: Anopheles arabiensis (Diptera: Culicidae) is a potential malaria vector commonly present at low altitudes in remote areas in Reunion Island. Little attention has been paid to the environmental conditions driving larval development and abundance patterns in potential habitats. Two field surveys were designed to determine whether factors that discriminate between aquatic habitats with an ...
DOI:: 10.1186/1756-3305-5-96
PubMed:: 22608179
PubMed Central:: PMC3461495
: https://doi.org/10.1186/1756-3305-5-96

88. Abl Family Kinases Modulate T Cell–Mediated Inflammation and Chemokine-Induced Migration Through the Adaptor HEF1 and the GTPase Rap1

Author:: Jing Jin Gu; Catherine P. Lavau; Elena Pugacheva; Erik J. Soderblom; M. Arthur Moseley; Ann Marie Pendergast
Source:: Science signaling 2012 v.5 no.233 pp. ra51
ISSN:: 1937-9145
Subject:: T-lymphocytes; actin; cell movement; chemokine receptors; chemokines; guanosinetriphosphatase; homeostasis; immunity; inflammation; lymphatic system; mice; monitoring; phosphotransferases (kinases); tyrosine
Abstract:: ... En’Abl’ing T Cell Migration To perform their role in immune surveillance and protective immunity, T cells must continuously circulate through the blood and secondary lymphoid organs (during homeostasis), as well as migrate to sites of inflammation. T cell migration is stimulated by chemokines, which activate small GTPases, including Rap1, that lead to cellular polarization and actin rearrangement. ...
DOI:: 10.1126/scisignal.2002632
PubMed:: 22810897
PubMed Central:: PMC3602906
: https://doi.org/10.1126/scisignal.2002632

89. Ablation of neurons expressing agouti-related protein, but not melanin concentrating hormone, in leptin-deficient mice restores metabolic functions and fertility

Author:: Qi Wu; Benjamin B. Whiddon; Richard D. Palmiter
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.8 pp. 3155-3160
ISSN:: 0027-8424
Subject:: anorexia; body weight; food intake; glucose tolerance; ingestion; melanin; metabolic syndrome; mice; neurons
Abstract:: ... Leptin-deficient (Lepob/ob) mice are obese, diabetic, and infertile. Ablation of neurons that make agouti-related protein (AgRP) in moderately obese adult Lepob/ob mice caused severe anorexia. The mice stopped eating for 2 wk and then gradually recovered. Their body weight fell to within a normal range for WT mice, at which point food intake and glucose tolerance were restored to that of WT mice. ...
DOI:: 10.1073/pnas.1120501109
PubMed:: 22232663
PubMed Central:: PMC3286929
: https://doi.org/10.1073/pnas.1120501109

90. Abnormal Segregation of Alleles and Haplotypes at the Polymorphic Site of the PRNP Gene Within Promoter and Intron 1 Regions in Polish Holstein–Friesian Cattle

Author:: Janusz Strychalski; Urszula Czarnik; Tadeusz Zabolewicz
Source:: Biochemical genetics 2012 v.50 no.7-8 pp. 520-528
ISSN:: 0006-2928
Subject:: alleles; cattle; haplotypes; homozygosity; introns; parents; progeny
Abstract:: ... Allele and haplotype segregation at the polymorphic sites within the promoter (23indel) and intron 1 (12indel) regions of the PRNP gene was analyzed in Polish Holstein–Friesian cattle. More 23del/del homozygotes and fewer 23ins/ins homozygotes than expected were observed in the offspring of ♂ 23ins/del × ♀ 23ins/del parents. In the offspring of ♂ 23ins/del × ♀ 23del/del parents and ♂ 23del/del × ♀ ...
DOI:: 10.1007/s10528-012-9496-2
PubMed:: 22258312
PubMed Central:: PMC3392501
: https://doi.org/10.1007/s10528-012-9496-2

91. Aboriginal hunting buffers climate-driven fire-size variability in Australia’s spinifex grasslands

Author:: Rebecca Bliege Bird; Brian F. Codding; Peter G. Kauhanen; Douglas W. Bird
Source:: Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.26 pp. 10287-10292
ISSN:: 0027-8424
Subject:: arid zones; burning; ecosystems; fire regime; fire spread; grasslands; hunters; issues and policy; land management; landscapes; lightning; mortality; risk; wet-dry cycles; wildfires; Australia
Abstract:: ... Across diverse ecosystems, greater climatic variability tends to increase wildfire size, particularly in Australia, where alternating wet–dry cycles increase vegetation growth, only to leave a dry overgrown landscape highly susceptible to fire spread. Aboriginal Australian hunting fires have been hypothesized to buffer such variability, mitigating mortality on small-mammal populations, which have ...
DOI:: 10.1073/pnas.1204585109
PubMed:: 22689979
PubMed Central:: PMC3387077
: https://doi.org/10.1073/pnas.1204585109

92. The AbrB2 Autorepressor, Expressed from an Atypical Promoter, Represses the Hydrogenase Operon To Regulate Hydrogen Production in Synechocystis Strain PCC6803

Author:: Jérémy Dutheil; Panatda Saenkham; Samer Sakr; Christophe Leplat; Marcia Ortega-Ramos; Hervé Bottin; Laurent Cournac; Corinne Cassier-Chauvat; Franck Chauvat
Source:: Journal of bacteriology 2012 v.194 no.19 pp. 5423-5433
ISSN:: 0021-9193
Subject:: Synechocystis; cell growth; ferredoxin hydrogenase; homeotic genes; hydrogen production; mutants; mutation; operon; plasmids
Abstract:: ... We have thoroughly investigated the abrB2 gene (sll0822) encoding an AbrB-like regulator in the wild-type strain of the model cyanobacterium Synechocystis strain PCC6803. We report that abrB2 is expressed from an active but atypical promoter that possesses an extended −10 element (TGTAATAT) that compensates for the absence of a −35 box. Strengthening the biological significance of these data, we f ...
DOI:: 10.1128/JB.00543-12
PubMed:: 22865847
PubMed Central:: PMC3457224
: https://doi.org/10.1128/JB.00543-12

93. Abrupt Temporal Fluctuations in the Chicken Fecal Microbiota Are Explained by Its Gastrointestinal Origin

Author:: M. Sekelja; I. Rud; S. H. Knutsen; V. Denstadli; B. Westereng; T. Næs; K. Rudi
Source:: Applied and environmental microbiology 2012 v.78 no.8 pp. 2941-2948
ISSN:: 0099-2240
Subject:: broiler chickens; ribosomal RNA; gizzard; cecum; pathogens; Shigella; feces composition; multivariate analysis; Escherichia; small intestine; human food chain; bacteria; colon
Abstract:: ... One of the main challenges in understanding the composition of fecal microbiota is that it can consist of microbial mixtures originating from different gastrointestinal (GI) segments. Here, we addressed this challenge for broiler chicken feces using a direct 16S rRNA gene-sequencing approach combined with multivariate statistical analyses. Broiler feces were chosen because of easy sampling and the ...
DOI:: 10.1128/AEM.05391-11
PubMed:: 22307311
PubMed Central:: PMC3318845
: https://doi.org/10.1128/AEM.05391-11

94. An Abscisic Acid-AtNAP Transcription Factor-SAG113 Protein Phosphatase 2C Regulatory Chain for Controlling Dehydration in Senescing Arabidopsis Leaves

Author:: Kewei Zhang; Su-Sheng Gan
Source:: Plant physiology 2012 v.158 no.2 pp. 961-969
ISSN:: 0032-0889
Subject:: stomatal movement; transcription factors; Arabidopsis; phenotype; phosphoprotein phosphatase; yeasts; Saccharomyces cerevisiae; gene targeting; genes; abscisic acid; site-directed mutagenesis; promoter regions; leaves
Abstract:: ... AtNAP is a NAC family transcription factor gene that plays a key role in leaf senescence but its underlying mechanisms are not known. SENESCENCE-ASSOCIATED GENE113 (SAG113), a gene encoding a Golgi-localized protein phosphatase 2C family protein phosphatase, mediates abscisic acid (ABA)-regulated stomatal movement and water loss specifically during leaf senescence. Here we report that SAG113 is a ...
DOI:: 10.1104/pp.111.190876
PubMed:: 22184656
PubMed Central:: PMC3271781
: https://doi.org/10.1104/pp.111.190876

95. Abscisic acid and CO2 signalling via calcium sensitivity priming in guard cells, new CDPK mutant phenotypes and a method for improved resolution of stomatal stimulus–response analyses

Author:: Katharine E. Hubbard; Robert S. Siegel; Gabriel Valerio; Benjamin Brandt; Julian I. Schroeder
Source:: Annals of botany 2012 v.109 no.1 pp. 5-17
ISSN:: 0305-7364
Subject:: Arabidopsis thaliana; guard cells; carbon dioxide; ion channels; stomatal movement; protein kinases; phenotype; calcium; gas exchange; genome; models; mutation; signal transduction; mutants; plasma membrane; abscisic acid
Abstract:: ... BACKGROUND: Stomatal guard cells are the regulators of gas exchange between plants and the atmosphere. Ca2+-dependent and Ca2+-independent mechanisms function in these responses. Key stomatal regulation mechanisms, including plasma membrane and vacuolar ion channels have been identified and are regulated by the free cytosolic Ca2+ concentration ([Ca2+]cyt). SCOPE: Here we show that CO2-induced sto ...
DOI:: 10.1093/aob/mcr252
PubMed:: 21994053
PubMed Central:: PMC3241576
: https://doi.org/10.1093/aob/mcr252

96. Absence of Disparities in the Quality of Primary Diabetes Care for South Asians and Chinese in an Urban Canadian Setting

Author:: Baiju R. Shah; Karen Cauch-Dudek; Sonia S. Anand; Peter C. Austin; Douglas G. Manuel; Janet E. Hux
Source:: Diabetes care 2012 v.35 no.4 pp. 794-796
ISSN:: 0149-5992
Subject:: Asians; blood pressure; low density lipoprotein cholesterol; minorities (people); noninsulin-dependent diabetes mellitus; patients; physicians
Abstract:: ... OBJECTIVE: To examine whether quality of diabetes care is equitable for South Asian and Chinese patients in an urban Canadian setting. RESEARCH DESIGN AND METHODS: Process and intermediate measures of quality of care were compared between 246 South Asians, 170 Chinese, and 431 patients from the general population with type 2 diabetes selected from 45 family physicians’ practices. RESULTS: A total ...
DOI:: 10.2337/dc11-1845
PubMed:: 22323411
PubMed Central:: PMC3308276
: https://doi.org/10.2337/dc11-1845

97. Absence of Livestock-Associated Methicillin-Resistant Staphylococcus aureus Clonal Complex CC398 as a Nasal Colonizer of Pigs Raised in an Alternative System

Author:: Christiane Cuny; Alexander W. Friedrich; Wolfgang Witte
Source:: Applied and environmental microbiology 2012 v.78 no.4 pp. 1296-1297
ISSN:: 0099-2240
Subject:: Staphylococcus aureus; alternative farming; farms; humans; nose; swine
Abstract:: ... Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) ST398 isolated from pigs raised in conventional farms was previously reported. Here we report a study on 25 farms adhering to an alternative system. LA-MRSA ST398 was not detected in nasal swabs from 178 pigs or from 89 humans working and living on these farms. ...
DOI:: 10.1128/AEM.07260-11
PubMed:: 22156420
PubMed Central:: PMC3273000
: https://doi.org/10.1128/AEM.07260-11

98. Absence of dry season Plasmodium parasitaemia, but high rates of reported acute respiratory infection and diarrhoea in preschool-aged children in Kaédi, southern Mauritania

Author:: Sunkaru Touray; Hampâté Bâ; Ousmane Bâ; Mohamedou Koïta; Cheikh B Ould Ahmed Salem; Moussa Keïta; Doulo Traoré; Ibrahima Sy; Mirko S Winkler; Jürg Utzinger; Guéladio Cissé
Source:: Parasites & vectors 2012 v.5 no.1 pp. 193
ISSN:: 1756-3305
Subject:: Culicidae; Plasmodium; blood; climate change; cross-sectional studies; diagnostic techniques; diarrhea; dry season; epidemiology; households; malaria; parasitemia; parasites; preschool children; questionnaires; rain; rivers; surveys; temperature; wet season; Mauritania; Senegal
Abstract:: ... BACKGROUND: The epidemiology of malaria in the Senegal River Gorgol valley, southern Mauritania, requires particular attention in the face of ongoing and predicted environmental and climate changes. While “malaria cases” are reported in health facilities throughout the year, past and current climatic and ecological conditions do not favour transmission in the dry season (lack of rainfall and very ...
DOI:: 10.1186/1756-3305-5-193
PubMed:: 22958321
PubMed Central:: PMC3465244
: https://doi.org/10.1186/1756-3305-5-193

99. Absolute Configuration of Isosilybin A by X-ray Crystallography of the Heavy Atom Analogue 7-(4-Bromobenzoyl)isosilybin A

Author:: Sy-Cordero Arlene A.; Day Cynthia S.; Oberlies Nicholas H.
Source:: Journal of natural products 2012 v.75 no.11 pp. 1879-1881
ISSN:: 1520-6025
Subject:: Silybum marianum; X-ray diffraction; chemical structure; chlorides; circular dichroism spectroscopy; fruits; silymarin
Abstract:: ... Isosilybin A (1) is one of the major flavonolignans that constitute silymarin, an extract of the fruits (achenes) of milk thistle (Silybum marianum). The chemistry of the Silybum flavonolignans has been studied for over four decades, and the absolute configuration of 1 has been determined previously by electronic circular dichroism and X-ray crystallography via correlating the relative configurati ...
DOI:: 10.1021/np3005369
PubMed:: 23116206
PubMed Central:: PMC3620721
: https://doi.org/10.1021/np3005369

100. Absolute Free Energy of Binding of Avidin/Biotin, Revisited

Author:: General Ignacio J.; Dragomirova Ralitsa; Meirovitch Hagai
Source:: Journal of physical chemistry 2012 v.116 no.23 pp. 6628-6636
ISSN:: 1520-5207
Subject:: Gibbs free energy; avidin; biotin; ligands; models; molecular dynamics; solvents
Abstract:: ... The binding of biotin to avidin is one of the strongest in nature with absolute free energy of binding, ΔA⁰ = −20.4 kcal/mol. Therefore, this complex became a target for a large number of computational studies, which all, however, are based on approximate techniques or simplified models and have led to a wide range of results Therefore, ΔA⁰ is calculated here by rigorous statistical mechanical met ...
DOI:: 10.1021/jp212276m
PubMed:: 22300239
PubMed Central:: PMC3383089
: https://doi.org/10.1021/jp212276m

PubAg

Main content area

Limit your search

Text Availability

Associated Data

Journal

Publication Year

Subject

Search

22,628 Search Results

Search Results

1. "Evolution Canyon," a potential microscale monitor of global warming across life

2. "tet(U)" Is Not a Tetracycline Resistance Determinant

3. (n-3) Fatty Acids and Cardiovascular Health: Are Effects of EPA and DHA Shared or Complementary?

4. (φ,ψ)₂ Motifs: A Purely Conformation-Based Fine-Grained Enumeration of Protein Parts at the Two-Residue Level

5. 1,2,3-Triazole–Heme Interactions in Cytochrome P450: Functionally Competent Triazole–Water–Heme Complexes

6. The 1,2-Benzenedithiole-Based Cyclocondensation Assay: A Valuable Tool for the Measurement of Chemopreventive Isothiocyanates

7. 1,4-Diazaspiro[2.2]pentanes as a Flexible Platform for the Synthesis of Diamine-Bearing Stereotriads

8. 1,500 year quantitative reconstruction of winter precipitation in the Pacific Northwest

9. 1-Methylthio-d-xylulose 5-Phosphate Methylsulfurylase: A Novel Route to 1-Deoxy-d-xylulose 5-Phosphate in Rhodospirillum rubrum

10. The 10-Year Cost-Effectiveness of Lifestyle Intervention or Metformin for Diabetes Prevention: An intent-to-treat analysis of the DPP/DPPOS

11. 100% Orange juice consumption is associated with better diet quality, improved nutrient adequacy, decreased risk for obesity, and improved biomarkers of health in adults: National Health and Nutrition Examination Survey, 2003-2006

12. IL- 10–induced microRNA-187 negatively regulates TNF-α, IL-6, and IL-12p40 production in TLR4-stimulated monocytes

13. 15N-Labeled Brain Enables Quantification of Proteome and Phosphoproteome in Cultured Primary Neurons

14. 15N-Labeled Full-Length Apolipoprotein E4 as an Internal Standard for Mass Spectrometry Quantification of Apolipoprotein E Isoforms

15. 17 novel polymorphic microsatellite markers for the giant water bug, Abedus herberti (Belostomatidae)

16. 1H Dynamic Nuclear Polarization Based on an Endogenous Radical

17. 1α,25-Dihydroxyvitamin D₃ and its analog, 2-methylene-19-nor-(20S)-1α,25-dihydroxyvitamin D₃ (2MD), suppress intraocular pressure in non-human primates

18. 1’-Acetoxychavicol acetate inhibits growth of human oral carcinoma xenograft in mice and potentiates cisplatin effect via proinflammatory microenvironment alterations

19. 2,2′,3,5′,6-Pentachlorobiphenyl (PCB 95) and Its Hydroxylated Metabolites Are Enantiomerically Enriched in Female Mice

20. 2,3-Hexanediols as Sex Attractants and a Female-produced Sex Pheromone for Cerambycid Beetles in the Prionine Genus Tragosoma

21. The 21.5-kDa isoform of myelin basic protein has a non-traditional PY-nuclear-localization signal

22. 25-Hydroxyvitamin D Concentrations and In Vivo Insulin Sensitivity and β-Cell Function Relative to Insulin Sensitivity in Black and White Youth

23. 25-Hydroxyvitamin D deficiency is associated with fatal stroke among whites but not blacks: The NHANES-III linked mortality files

24. 26S Proteasome regulation of Ankrd1/CARP in adult rat ventricular myocytes and human microvascular endothelial cells

25. The 27–year decline of coral cover on the Great Barrier Reef and its causes

26. The 2b proteins of Cucumber mosaic virus generally have the potential to differentially induce necrosis on Arabidopsis

27. 3-Helix Micelles Stabilized by Polymer Springs

28. IL- 33 is processed into mature bioactive forms by neutrophil elastase and cathepsin G

29. IL- 38 binds to the IL-36 receptor and has biological effects on immune cells similar to IL-36 receptor antagonist

30. 3D imaging and mechanical modeling of helical buckling in Medicago truncatula plant roots

31. A 3D localized surface plasmon resonance biosensor for the study of trivalent arsenic binding to the ArsA ATPase

32. 3D superhydrophobic electrospun meshes as reinforcement materials for sustained local drug delivery against colorectal cancer cells

33. 4-Hydroxynonenal differentially regulates adiponectin gene expression and secretion via activating PPARγ and accelerating ubiquitin–proteasome degradation

34. 4-O-methylation of glucuronic acid in Arabidopsis glucuronoxylan is catalyzed by a domain of unknown function family 579 protein

35. 470-Million-year-old black corals from China

36. 5-Carboxylcytosine is localized to euchromatic regions in the nuclei of follicular cells in axolotl ovary

37. 5-Hydroxymethylcytosine – the elusive epigenetic mark in mammalian DNA

38. 500,000 fish phenotypes: The new informatics landscape for evolutionary and developmental biology of the vertebrate skeleton

39. A 54-Kilodalton Protein Encoded by pBtoxis Is Required for Parasporal Body Structural Integrity in Bacillus thuringiensis subsp. israelensis

40. 5′-Terminal chemical capping of spliced leader RNAs

41. 5′UTR-intron of the Gladiolus polyubiquitin promoter GUBQ1 enhances translation efficiency in Gladiolus and Arabidopsis

42. The 60-Kilodalton Protein Encoded by orf2 in the cry19A Operon of Bacillus thuringiensis subsp. jegathesan Functions Like a C-Terminal Crystallization Domain

43. 6′-Oxa analogs of S-adenosylhomocysteine

44. IL- 7 receptor blockade reverses autoimmune diabetes by promoting inhibition of effector/memory T cells

45. 7,8,3′-Trihydroxyflavone, a potent small molecule TrkB receptor agonist, protects spiral ganglion neurons from degeneration both in vitro and in vivo

46. The 70S ribosome modulates the ATPase activity of Escherichia coli YchF

47. 9,400 years of cosmic radiation and solar activity from ice cores and tree rings

48. 9-Mercaptodethiobiotin Is Generated as a Ligand to the [2Fe–2S]+ Cluster during the Reaction Catalyzed by Biotin Synthase from Escherichia coli

49. An A/ENTH Domain-Containing Protein Functions as an Adaptor for Clathrin-Coated Vesicles on the Growing Cell Plate in Arabidopsis Root Cells

50. AAA ATPase p97/VCP is essential for TRIM21-mediated virus neutralization

51. ABA signal in rice under stress conditions

52. The ABCs of molecular dynamics simulations on B-DNA, circa 2012

53. ACCELERATED CELL DEATH 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis

54. The ADAR protein family

55. AGL24 acts in concert with SOC1 and FUL during Arabidopsis floral transition

56. AGNPS GIS-Based Tool for Watershed-Scale Identification and Mapping of Cropland Potential Ephemeral Gullies

57. The AINTEGUMENTA LIKE1 Homeotic Transcription Factor PtAIL1 Controls the Formation of Adventitious Root Primordia in Poplar

58. AMP-activated protein kinase stimulates myostatin expression in C2C12 cells

59. The APC/C Subunit Mnd2/Apc15 Promotes Cdc20 Autoubiquitination and Spindle Assembly Checkpoint Inactivation

60. APOA5 genotype modulates 2-y changes in lipid profile in response to weight-loss diet intervention: the Pounds Lost Trial

61. APOBEC2 Is a Monomer in Solution: Implications for APOBEC3G Models

62. The AR dependent cell cycle: Mechanisms and cancer relevance

63. ARABIDOPSIS TRITHORAX-RELATED3/SET DOMAIN GROUP2 is Required for the Winter-Annual Habit of Arabidopsis thaliana

64. ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting

65. The ARP2/3 Complex Mediates Guard Cell Actin Reorganization and Stomatal Movement in Arabidopsis

66. ATH1 and KNAT2 proteins act together in regulation of plant inflorescence architecture

67. ATHB4 and HAT3, two class II HD-ZIP transcription factors, control leaf development in Arabidopsis

68. ATI1, a newly identified atg8-interacting protein, binds two different Atg8 homologs

69. ATM kinase inhibition in glial cells activates the innate immune response and causes neurodegeneration in Drosophila