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You searched for: Publication Year 2011 Remove constraint Publication Year: 2011 Subject Escherichia coli O157 Remove constraint Subject: Escherichia coli O157
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1. 3-Indolylacetonitrile Decreases Escherichia coli O157:H7 Biofilm Formation and Pseudomonas aeruginosa Virulence

Author:
Lee, Jin-Hyung; Cho, Moo Hwan; Lee, Jintae
Source:
Environmental microbiology 2011 v.13 no.1 pp. 62-73
ISSN:
1462-2912
Subject:
Escherichia coli O157; Pseudomonas aeruginosa; auxins; bacteria; biofilm; genes; glycerol; metabolism; microarray technology; microbial communities; oxygenases; virulence
Abstract:
... Intercellular signal indole and its derivative hydroxyindoles inhibit Escherichia coli biofilm and diminish Pseudomonas aeruginosa virulence. However, indole and bacterial indole derivatives are unstable in the microbial community because they are quickly degraded by diverse bacterial oxygenases. Hence, this work sought to identify novel, non-toxic, stable and potent indole derivatives from plant ...
DOI:
10.1111/j.1462-2920.2010.02308.x
PubMed:
20649646

2. Active packaging of ground beef patties by edible zein films incorporated with partially purified lysozyme and Na₂EDTA

Author:
Ünalan, İlke Uysal; Korel, Figen; Yemenicioğlu, Ahmet
Source:
International journal of food science & technology 2011 v.46 no.6 pp. 1289-1295
ISSN:
0950-5423
Subject:
antimicrobial properties; lysozyme; packaging; patties; ground beef; Salmonella Typhimurium; EDTA (chelating agent); Listeria monocytogenes; Escherichia coli O157; edible films; zein
Abstract:
... In this study, antimicrobial activity of zein films incorporated with partially purified lysozyme and disodium ethylenediaminetetraacetic acid (Na₂EDTA) has been tested on selected pathogenic bacteria and refrigerated ground beef patties. The developed films containing 700 μg cm⁻² lysozyme and 300 μg cm⁻² Na₂EDTA showed antimicrobial activity on Listeria monocytogenes, Escherichia coli O157:H7, an ...
DOI:
10.1111/j.1365-2621.2011.02625.x

3. Adherence of Escherichia coli O157:H7 to epithelial cells in vitro and in pig gut loops is affected by bacterial culture conditions

Author:
Yin, Xianhua; Feng, Yanni; Wheatcroft, Roger; Chambers, James; Gong, Joshua; Gyles, Carlton L.
Source:
Canadian journal of veterinary research 2011 v.75 no.2 pp. 81-88
ISSN:
0830-9000
Subject:
Escherichia coli O157; bacteria; bacterial adhesion; colon; enterocytes; ileum; pH; swine
Abstract:
... The objectives of this study were to determine the effect of bacterial culture conditions on adherence of enterohemorrhagic Escherichia coli (EHEC) O157:H7 strain 86-24 in vivo to pig enterocytes and to compare the results with adherence in vitro to cultured HEp-2 and IPEC-J2 cells. Growth of O157:H7 in MacConkey broth (MB) resulted in almost no adherence to both HEp-2 and IPEC-J2 cells; prior exp ...

4. Advantages and challenges of increased antimicrobial copper use and copper mining

Author:
Jutta Elguindi; Xiuli Hao; Yanbing Lin; Hend A. Alwathnani; Gehong Wei; Christopher Rensing
Source:
Applied microbiology and biotechnology 2011 v.91 no.2 pp. 237-249
ISSN:
0175-7598
Subject:
Clostridium difficile; Cronobacter sakazakii; Escherichia coli O157; Staphylococcus aureus; alloys; bacteria; bacterial infections; copper; cross infection; food contamination; food production; fruits; groundwater contamination; humans; leaching; mine spoil; mining; phytoremediation; toxicity; vegetables
Abstract:
... Copper is a highly utilized metal for electrical, automotive, household objects, and more recently as an effective antimicrobial surface. Copper-containing solutions applied to fruits and vegetables can prevent bacterial and fungal infections. Bacteria, such as Salmonellae and Cronobacter sakazakii, often found in food contamination, are rapidly killed on contact with copper alloys. The antimicrob ...
DOI:
10.1007/s00253-011-3383-3
PubMed:
21656137

5. Antibacterial Activity and Mechanism of Action of Zinc Oxide Nanoparticles against Campylobacter jejuni

Author:
Xie, Yanping; He, Yiping; Irwin, Peter L.; Jin, Tony; Shi, Xianming
Source:
Applied and environmental microbiology 2011 v.77 no.7 pp. 2325-2331
ISSN:
0099-2240
Subject:
mechanism of action; cell growth; Salmonella Enteritidis; oxidative stress; stress response; polymerase chain reaction; gene expression; zinc oxide; scanning electron microscopy; cell membranes; genes; Escherichia coli O157; nanoparticles; Campylobacter jejuni; antibacterial properties
Abstract:
... The antibacterial effect of zinc oxide (ZnO) nanoparticles on Campylobacter jejuni was investigated for inhibition and inactivation of cell growth. The results showed that C. jejuni was extremely sensitive to treatment with ZnO nanoparticles. The MIC of ZnO nanoparticles for C. jejuni was determined to be 0.05 to 0.025 mg/ml, which is 8- to 16-fold lower than that for Salmonella enterica serovar E ...
Handle:
10113/59162
DOI:
10.1128/AEM.02149-10
PubMed:
21296935
PubMed Central:
PMC3067441

6. Antibacterial activity of naringin derivatives against pathogenic strains

Author:
G. Céliz; M. Daz; M.C. Audisio
Source:
Journal of applied microbiology 2011 v.111 no.3 pp. 731-738
ISSN:
1364-5072
Subject:
Citrus; Escherichia coli O157; agar; anti-infective agents; antibacterial properties; bacteria; carbon; chemical structure; esters; fatty acids; guidelines; industry; naringenin; naringin; sugars; viability; virulent strains; wastes
Abstract:
... Aims: To study the antimicrobial activity of naringin (NAR), a flavonoid extracted from citrus industry waste, and NAR derivatives [naringenin (NGE), prunin and alkyl prunin esters] against pathogenic bacteria such as L. monocytogenes, E. coli O157:H7 and S. aureus. The relationship between the structure of the chemical compounds and their antagonistic effect was also analysed. Methods and Resu ...
DOI:
10.1111/j.1365-2672.2011.05070.x

7. Antibacterial activity of oregano and thyme essential oils against Listeria monocytogenes and Escherichia coli O157:H7 in feta cheese packaged under modified atmosphere

Author:
Alexander Govaris; Evropi Botsoglou; Daniil Sergelidis; Pashalina S. Chatzopoulou
Source:
Lebensmittel-Wissenschaft + Technologie 2011 v.44 no.4 pp. 1240-1244
ISSN:
0023-6438
Subject:
Escherichia coli O157; Listeria monocytogenes; antibacterial properties; carbon dioxide; cold storage; controlled atmosphere storage; essential oils; feta cheese; modified atmosphere packaging; oregano; thyme; thymol
Abstract:
... The antibacterial activity of the essential oils (EO) of oregano and thyme added at doses of 0.1 or 0.2 and 0.1 ml/100 g, respectively, to feta cheese inoculated with Escherichia coli O157:H7 or Listeria monocytogenes was investigated during cheese storage under modified atmosphere packaging (MAP) of 50% CO₂ and 50% N₂ at 4 °C. Compositional analysis showed that the predominant phenols wer ...
DOI:
10.1016/j.lwt.2010.09.022

8. Antibiosis of vineyard ecosystem fungi against food-borne microorganisms

Author:
Carolina Cueva; M. Victoria Moreno-Arribas; Begoña Bartolomé; Óscar Salazar; M. Francisca Vicente; Gerald F. Bills
Source:
Research in microbiology 2011 v.162 no.10 pp. 1043-1051
ISSN:
0923-2508
Subject:
Acinetobacter baumannii; Candida albicans; Escherichia coli O157; Gram-negative bacteria; Gram-positive bacteria; Hypocreales; Lactobacillus brevis; Lactobacillus plantarum; Pleosporales; Pseudomonas aeruginosa; Staphylococcus aureus; Xylariales; anti-infective properties; antibiosis; ecosystems; fermentation; food pathogens; food spoilage; foods; fungi; internal transcribed spacers; liquid chromatography; mass spectrometry; metabolites; probiotics; ribosomal RNA; sequence analysis; vineyards
Abstract:
... Fermentation extracts from fungi isolated from vineyard ecosystems were tested for antimicrobial activities against a set of test microorganisms, including five food-borne pathogens (Staphylococcus aureus EP167, Acinetobacter baumannii (clinically isolated), Pseudomonas aeruginosa PAO1, Escherichia coli O157:H7 (CECT 5947) and Candida albicans MY1055) and two probiotic bacteria (Lactobacillus plan ...
DOI:
10.1016/j.resmic.2011.09.002

9. Antimicrobial Effect of Guava on Escherichia Coli O157:H7 and Salmonella Typhimurium in Liquid Medium

Author:
Salam A. Ibrahim; Guochen Yang; Danfeng Song; Tom S.F. Tse
Source:
International journal of food properties 2011 v.14 no.1 pp. 102-109
ISSN:
1094-2912
Subject:
antimicrobial properties; minimum inhibitory concentration; Salmonella Typhimurium; growth retardation; guavas; Escherichia coli O157; anti-infective agents; food safety
Abstract:
... This study investigated the antimicrobial effects of guava against E. coli O157:H7 and Salmonella in liquid medium. The minimum inhibitory concentration (MIC) and the minimum lethal concentration (MLC) values for each bacterial strain were recorded. In addition, the growth of the individual strain and a combined mixture of the strains in liquid medium over time were assessed. Guava was found to in ...
DOI:
10.1080/10942910903147833

10. Antimicrobial activity of ascorbic acid alone or in combination with lactic acid on Escherichia coli O157:H7 in laboratory medium and carrot juice

Author:
Mehrdad Tajkarimi; Salam A. Ibrahim
Source:
Food control 2011 v.22 no.6 pp. 801-804
ISSN:
0956-7135
Subject:
Escherichia coli O157; antimicrobial properties; ascorbic acid; bacteria; carrot juice; lactic acid; models
Abstract:
... The antimicrobial effects of ascorbic acid alone and in combination with lactic acid, against Escherichia coli O157:H7 in Brain Heart Infusion (BHI) broth and in carrot juice as a food model was investigated. In control samples, E. coli O157:H7 continued to growth from 3.98 ± 0.2 log CFU and reached to 8.88 ± 0.1 log₁₀ CFU after 8 h at 37° C; however, bacterial population was undetectable level in ...
DOI:
10.1016/j.foodcont.2010.11.030

11. Antimicrobial and antioxidant activities of lignin from residue of corn stover to ethanol production

Author:
Xin Dong; Meidui Dong; Yingjian Lu; Alexandra Turley; Tony Jin; Changqing Wu
Source:
Industrial crops and products 2011 v.34 no.3 pp. 1629-1634
ISSN:
0926-6690
Subject:
Gram-negative bacteria; antimicrobial properties; Staphylococcus aureus; viability; Salmonella Enteritidis; absorbance; Yarrowia lipolytica; yeasts; biomass production; bacteriophages; byproducts; ethanol production; Fourier transform infrared spectroscopy; corn stover; Listeria monocytogenes; Escherichia coli O157; temperature; antioxidant activity; lignin; Gram-positive bacteria
Abstract:
... To improve the economic viability of the biofuel production from biomass resource, a value-added lignin byproduct from this process is increasingly important. Antioxidant and antimicrobial activities of lignin extracted from residue of corn stover to ethanol production were investigated. The lignin extracts exhibited strong antioxidant activities in hydrophilic oxygen radical absorbance capacity ( ...
DOI:
10.1016/j.indcrop.2011.06.002

12. Antimicrobial effect of cranberry juice and extracts

Author:
J. Côté; S. Caillet; G. Doyon; D. Dussault; J.-F. Sylvain; M. Lacroix
Source:
Food control 2011 v.22 no.8 pp. 1413-1418
ISSN:
0956-7135
Subject:
Pseudomonas aeruginosa; Staphylococcus aureus; juices; minimum inhibitory concentration; Enterococcus faecium; Salmonella Typhimurium; phenol; cranberries; Listeria monocytogenes; Escherichia coli O157; antibacterial properties
Abstract:
... The antimicrobial effect of cranberry juice and of three cranberry extracts (water-soluble (E1) and apolar phenolic compounds (E2), and anthocyanins (E3)) was investigated against seven bacterial strains (Enterococcus faecium resistant to vancomycin (ERV), Escherichia coli O157:H7 EDL 933, Escherichia coli ATCC 25922, Listeria monocytogenes HPB 2812, Pseudomonas aeruginosa ATCC 15442, Salmonella T ...
DOI:
10.1016/j.foodcont.2011.02.024

13. Antimicrobial effects of weak acids on the survival of Escherichia coli O157:H7 under anaerobic conditions

Author:
Lu, Huiying J.; Breidt, Frederick Jr.; Perez-Diaz, Ilenys M.; Osborne, Jason A.
Source:
Journal of food protection 2011 v.74 no.6 pp. 893-898
Subject:
acidity; foods; food packaging; malic acid; fruit products; lactic acid; mechanism of action; acid tolerance; bacterial contamination; benzoic acid; dissolved oxygen; anaerobic conditions; sorbic acid; food preservatives; antibacterial properties; vegetable products; viability; acetic acid; sulfite liquor; food pathogens; pH; Escherichia coli O157; fumaric acid
Abstract:
... Outbreaks of disease due to vegetative bacterial pathogens associated with acid foods (such as apple cider) have raised concerns about acidified vegetables and related products that have a similar pH (3.2 to 4.0). Escherichia coli O157:H7 and related strains of enterohemorrhagic E. coli (EHEC) have been identified as the most acid resistant vegetative pathogens in these products. Previous research ...
Handle:
10113/49548
DOI:
10.4315/0362-028X.JFP-10-404

14. Antimicrobial efficacy of lactoferrin, its amidated and pepsin‐digested derivatives, and their combinations, on Escherichia coli O157:H7 and Serratia liquefaciens

Author:
Del Olmo, A.; Calzada, J.; Nuñez, M.
Source:
Letters in applied microbiology 2011 v.52 no.1 pp. 9-14
ISSN:
0266-8254
Subject:
antimicrobial properties; Serratia; antibiotic resistance; food industry; lactoferrin; Escherichia coli O157; anti-infective agents; bacteria
Abstract:
... Aims: To evaluate the in vitro bactericidal efficacy of lactoferrin (LF), its amidated (AMILF) and pepsin‐digested (PDLF) derivatives, and their combinations, on Escherichia coli O157:H7 and Serratia liquefaciens. Methods and Results: PDLF exhibited the most potent bactericidal efficacy on E. coli O157:H7 (>2·5 log₁₀ CFU ml⁻¹ reduction at concentrations ≥1 mg ml⁻¹), and AMILF on Ser. liquefaciens ...
DOI:
10.1111/j.1472-765X.2010.02957.x
PubMed:
21070266

15. Antimicrobial, water vapour permeability, mechanical and thermal properties of casein based Zataraia multiflora Boiss. Extract containing film

Author:
Atefeh Broumand; Zahra Emam-Djomeh; Manouchehr Hamedi; Sayed Hadi Razavi
Source:
Lebensmittel-Wissenschaft + Technologie 2011 v.44 no.10 pp. 2316-2323
ISSN:
0023-6438
Subject:
Staphylococcus aureus; caseinates; Salmonella Typhimurium; essential oils; stearic acid; casein; scanning electron microscopy; Escherichia coli O157; edible films; water vapor; tensile strength; permeability
Abstract:
... Hydrophobic sodium caseinate based edible films were prepared by incorporating stearic and oleic acids. Films were produced with a protein/lipid ratio of 1.05. Antimicrobial films were produced based on the hydrophobic films by adding the essential oil of Zataraia multiflora Boiss. Water vapour permeability, microstructure, antimicrobial, mechanical and thermal properties of these films were studi ...
DOI:
10.1016/j.lwt.2011.07.002

16. Antioxidant and Antimicrobial Activities of Essential Oils Obtained from Oregano (Origanum vulgare ssp. hirtum) by Using Different Extraction Methods

Author:
Karakaya, Sibel; El, Sedef Nehir; Karagözlü, Nural; Şahin, Serpil
Source:
Journal of medicinal food 2011 v.14 no.6 pp. 645-652
ISSN:
1096-620X
Subject:
antimicrobial properties; Staphylococcus aureus; Salmonella Typhimurium; Origanum vulgare subsp. hirtum; essential oils; oregano; oils; Listeria monocytogenes; Escherichia coli O157; antioxidant activity; antioxidants; supercritical fluid extraction
Abstract:
... In this study, antioxidant and antimicrobial activities of essential oils obtained from oregano (Origanum vulgare ssp. hirtum) were determined by using solvent-free microwave extraction (SFME), supercritical fluid extraction, and conventional hydrodistillation (CH) methods. The inhibitory effects on the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical of essential oils obtained from ...
DOI:
10.1089/jmf.2010.0098
PubMed:
21314366

17. Application of acidic calcium sulfate and e-polylysine to pre-rigor beef rounds for reduction of pathogens

Author:
Robin C Anderson
Source:
Journal of food safety 2011 v.31 pp. 395-400
ISSN:
0149-6085
Subject:
mechanism of action; slaughter; stainless steel; beef carcasses; decontamination; lactic acid; Salmonella Typhimurium; beef; Listeria monocytogenes; Escherichia coli O157; anti-infective agents; pathogens; calcium sulfate
Abstract:
... This study evaluated the effectiveness of warm solutions of acidic calcium sulfate (ACS), lactic acid (LA), e-polylysine (EPL), ACS plus EPL and sterile distilled water (W) applied to the surface of fresh, pre-rigor beef rounds for reducing Salmonella Typhimurium, Escherichia coli O157:H7 and Listeria monocytogenes applied as a cocktail at a level of 6.4 log cfu/ 50 cm2. All treatments were applie ...
Handle:
10113/57283
DOI:
10.1111/j.1745-4565.2011.00312.x

18. Application of high hydrostatic pressure to decontaminate green onions from Salmonella and Escherichia coli O157:H7

Author:
Hudaa Neetoo; Sanaz Nekoozadeh; Zheng Jiang; Haiqiang Chen
Source:
Food microbiology 2011 v.28 no.7 pp. 1275-1283
ISSN:
0740-0020
Subject:
risk; dipping; Salmonella; pathogens; soaking; streptomycin; foodborne illness; color; green onions; Escherichia coli O157; heat treatment; inoculum; high pressure treatment
Abstract:
... Consumption of fecally contaminated green onions has been implicated in several major outbreaks of foodborne illness. The objectives of this study were to investigate the survival and growth of Salmonella and Escherichia coli O157:H7 in green onions during storage and to assess the application of high hydrostatic pressure (HHP) to decontaminate green onions from both pathogens. Bacterial strains r ...
DOI:
10.1016/j.fm.2011.05.005
PubMed:
21839376

19. Assessment of Escherichia coli O157:H7-specific bacteriophages e11/2 and e4/1c in model broth and hide environments

Author:
Brid Coffey; Lucia Rivas; Geraldine Duffy; Aidan Coffey; R. Paul Ross; Olivia McAuliffe
Source:
International journal of food microbiology 2011 v.147 no.3 pp. 188-194
ISSN:
0168-1605
Subject:
foods; biological control agents; cattle; exposure duration; water activity; bacteriophages; environmental factors; pH; sodium chloride; Escherichia coli O157; temperature; food chain
Abstract:
... The efficacy of bacteriophages e11/2 and e4/1c as potential biocontrol agents for Escherichia coli O157:H7 in food applications was assessed under conditions relevant to the food chain environment. The stability of each phage was determined following exposure to varying environmental conditions (pH, temperature, water activity, and sodium chloride) and the ability of each phage to infect and reduc ...
DOI:
10.1016/j.ijfoodmicro.2011.04.001
PubMed:
21531032

20. Assessment of carcass contamination with E. coli O157 before and after washing with water at abattoirs in Nigeria

Author:
M. Bello; M.K. Lawan; J.K.P. Kwaga; M.A. Raji
Source:
International journal of food microbiology 2011 v.150 no.2-3 pp. 184-186
ISSN:
0168-1605
Subject:
Escherichia coli O157; agglutination; beef carcasses; indicator species; latex; slaughterhouses; washing; Nigeria
Abstract:
... The study was carried out to assess the level of beef carcass contamination with Escherichia coli including O157 strains before and after washing with water. Samples of water used for washing carcasses were collected and thirty beef carcasses were swabbed within a period of one month in each of three abattoirs located in North-Western states of Nigeria. E. coli were enumerated as indicator organis ...
DOI:
10.1016/j.ijfoodmicro.2011.07.029
PubMed:
21843905

21. Assessment of microbial contamination levels of street-vended foods in Korea

Author:
Cho, Joon-Il; Cheung, Chi-Yeun; Lee, Sun-Mi; Ko, Soo-Il; Kim, Kyu-Heon; Hwang, In-Sun; Kim, Seung-Hwan; Cho, Soo-Yeol; Lim, Chul-Ju; Lee, Kwang-Ho; Kim, Keun-Sung; Ha, Sang-Do
Source:
Journal of food safety 2011 v.31 no.1 pp. 41-47
ISSN:
0149-6085
Subject:
Escherichia coli O157; Listeria monocytogenes; Salmonella; Staphylococcus aureus; Vibrio parahaemolyticus; bacterial contamination; consumer protection; disease control; food contamination; food intake; food intoxication; food processing plants; genes; good hygiene practices; microbiological quality; microbiological risk assessment; public health; ready-to-eat foods; street foods; toxigenic strains; virulence; Korean Peninsula
Abstract:
... This study was conducted to evaluate the microbial quality of street-vended foods in Korea. The microbiological contamination levels of aerobic mesophilic bacteria ranged from 0 to 7.43 log cfu/g with a mean value of 4.71 ± 1.53 log cfu/g, while the level of total coliforms ranged from 0 to 6.86 log cfu/g with a mean value of 3.24 ± 1.40 log cfu/g. Staphylococcus aureus (SA) and Escherichia coli ( ...
DOI:
10.1111/j.1745-4565.2010.00264.x

22. Associations among pathogenic bacteria, parasites, and environmental and land use factors in multiple mixed-use watersheds

Author:
G. Wilkes; T.A. Edge; V.P.J. Gannon; C. Jokinen; E. Lyautey; N.F. Neumann; N. Ruecker; A. Scott; M. Sunohara; E. Topp; D.R. Lapen
Source:
Water research 2011 v.45 no.18 pp. 5807-5825
ISSN:
0043-1354
Subject:
Campylobacter; Cryptosporidium; Escherichia coli O157; Giardia; Listeria monocytogenes; Salmonella enterica; air; bacteria; barns; cattle; cropland; farms; land use; manure spreading; oocysts; parasites; pastures; pathogens; rain; regression analysis; rivers; sediments; silage; soil water content; spring; streams; surface water; turbidity; water quality; water table; water temperature; watersheds; wildlife; winter; Ontario
Abstract:
... Over a five year period (2004–08), 1171 surface water samples were collected from up to 24 sampling locations representing a wide range of stream orders, in a river basin in eastern Ontario, Canada. Water was analyzed for Cryptosporidium oocysts and Giardia cyst densities, the presence of Salmonella enterica subspecies enterica, Campylobacter spp., Listeria monocytogenes, and Escherichia coli O157 ...
DOI:
10.1016/j.watres.2011.06.021
PubMed:
21889781

23. Assumptions of Acceptance Sampling and the Implications for Lot Contamination: Escherichia coli O157 in Lots of Australian Manufacturing Beef

Author:
Andreas Kiermeier; Glen Mellor; Robert Barlow; Ian Jenson
Source:
Journal of food protection 2011 v.74 no.4 pp. 539-544
ISSN:
0362-028X
Subject:
Escherichia coli O157; beef; binomial distribution; control methods; exports; grinding; manufacturing; microbiological quality; pathogens
Abstract:
... The aims of this work were to determine the distribution and concentration of Escherichia coli O157 in lots of beef destined for grinding (manufacturing beef) that failed to meet Australian requirements for export, to use these data to better understand the performance of sampling plans based on the binomial distribution, and to consider alternative approaches for evaluating sampling plans. For ea ...
DOI:
10.4315/0362-028X.JFP-10-497

24. Attachment and biofilm formation by Escherichia coli O157:H7 at different temperatures, on various food-contact surfaces encountered in beef processing

Author:
Dimitra Dourou; Catherine Simpson Beauchamp; Yohan Yoon; Ifigenia Geornaras; Keith E. Belk; Gary C. Smith; George-John E. Nychas; John N. Sofos
Source:
International journal of food microbiology 2011 v.149 no.3 pp. 262-268
ISSN:
0168-1605
Subject:
Escherichia coli O157; biofilm; cold storage; cross contamination; ground beef; pathogens; polyethylene; rendering; sanitation; stainless steel
Abstract:
... Escherichia coli O157:H7 attached to beef-contact surfaces found in beef fabrication facilities may serve as a source of cross-contamination. This study evaluated E. coli O157:H7 attachment, survival and growth on food-contact surfaces under simulated beef processing conditions. Stainless steel and high-density polyethylene surfaces (2×5cm) were individually suspended into each of three substrates ...
DOI:
10.1016/j.ijfoodmicro.2011.07.004
PubMed:
21802758

25. Biocontrol of Escherichia coli O157:H7 exerted by endogenous microflora and oleoresins in minimally processed lettuce and carrot

Author:
Alejandra Ponce; Rocio Avalo; Sara Inés Roura; María del Rosario Moreira
Source:
International journal of postharvest technology and innovation 2011 v.2 no.3 pp. 243-259
ISSN:
1744-7550
Subject:
Escherichia coli O157; antibacterial properties; biological control; carrots; lettuce; microorganisms; minimally processed foods; oleoresins; oregano; pathogens; rosemary; storage temperature
Abstract:
... The objectives of this study were to analyse the effectiveness of native microflora of lettuce and carrot in controlling Escherichia coli O157:H7 and to evaluate the technological application of oregano and rosemary to control E. coli in vegetables stored at 8°C and 20°C. The interaction between the background microflora of lettuce and carrot with E. coli O157:H7 was observed at 20°C. In samples s ...
DOI:
10.1504/IJPTI.2011.043324

26. Biocontrol of Listeria monocytogenes and Escherichia coli O157:H7 in Meat by Using Phages Immobilized on Modified Cellulose Membranes

Author:
H. Anany; W. Chen; R. Pelton; M. W. Griffiths
Source:
Applied and environmental microbiology 2011 v.77 no.18 pp. 6379-6387
ISSN:
0099-2240
Subject:
freeze drying; packaging; antibiotics; hosts; meat; bacteriophages; cellulose; industrial applications; methodology; pathogenicity; storage temperature; Listeria monocytogenes; Escherichia coli O157; raw meat; bacteria; biological control
Abstract:
... The ability of phages to specifically interact with and lyse their host bacteria makes them ideal antibacterial agents. The range of applications of bacteriophage can be extended by their immobilization on inert surfaces. A novel method for the oriented immobilization of bacteriophage has been developed. The method was based on charge differences between the bacteriophage head, which exhibits an o ...
DOI:
10.1128/AEM.05493-11
PubMed:
21803890
PubMed Central:
PMC3187159

27. Biogenic amine content and microbiological profile of Pecorino di Farindola cheese

Author:
Maria Schirone; Rosanna Tofalo; Giampiero Mazzone; Aldo Corsetti; Giovanna Suzzi
Source:
Food microbiology 2011 v.28 no.1 pp. 128-136
ISSN:
0740-0020
Subject:
Enterococcus; Escherichia coli O157; Lactobacillus; Listeria monocytogenes; Salmonella; Staphylococcus; Streptococcus thermophilus; bacteria; cheese milk; cheeses; chemical composition; conjugated linoleic acid; dairy farming; ewe milk; human health; hygiene; isomers; tyramine; yeasts; Italy
Abstract:
... “Pecorino di Farindola” is a traditional ewes’ milk cheese produced in the Abruzzo region (Italy) and ripened for a minimum of 90 days. The main objective of this research was to characterize the microbiological and chemical composition of this cheese, manufactured in ten dairy farms during the winter cheese-making season (December through March). By using classical enumeration system on specific ...
DOI:
10.1016/j.fm.2010.09.005
PubMed:
21056784

28. Bovine recto‐anal junction squamous epithelial (RSE) cell adhesion assay for studying Escherichia coli O157 adherence

Author:
I.T. Kudva; E.A. Dean‐Nystrom
Source:
Journal of applied microbiology 2011 v.111 no.5 pp. 1283-1294
ISSN:
1364-5072
Subject:
Escherichia coli O157; avirulent strains; bacteria; bacterial adhesion; cattle; food pathogens; gastrointestinal system; human cell lines; humans
Abstract:
... Aim: To develop a new adherence assay, using cattle recto‐anal junction squamous epithelial (RSE) cells, for evaluating bacterial adherence to cells of bovine origin. Methods and Results: Proof of concept was demonstrated using the human gastrointestinal pathogen Escherichia coli O157:H7, for which cattle are reservoirs. Adherence assays were conducted using both RSE and HEp‐2 cells, in the pr ...
DOI:
10.1111/j.1365-2672.2011.05139.x

29. Burger Preparation: What Consumers Say and Do in the Home

Author:
Ho S. Phang; Christine M. Bruhn
Source:
Journal of food protection 2011 v.74 no.10 pp. 1708-1716
ISSN:
0362-028X
Subject:
Escherichia coli O157; Food and Drug Administration Food Code; Salmonella; USDA; bacteria; compliance; consumer attitudes; cross contamination; food handling; food safety education; foodborne illness; ground beef; guidelines; hamburgers; hand washing; hands; households; internal temperature; questionnaires; salads; thermometers; volunteers; California
Abstract:
... Ground beef has been linked to outbreaks of pathogenic bacteria like Escherichia coli O157:H7 and Salmonella. Consumers may be exposed to foodborne illness through unsafe preparation of ground beef. Video footage of 199 volunteers in Northern California preparing hamburgers and salad was analyzed for compliance with U.S. Department of Agriculture recommendations and for violations of the U.S. Food ...
DOI:
10.4315/0362-028X.JFP-10-417

30. Characterization of a ViI-like phage specific to Escherichia coli O157:H7

Author:
Elizabeth M. Kutter; Kyobi Skutt-Kakaria; Bob Blasdel; Ayman El-Shibiny; Anna Castano; Daniel Bryan; Andrew M. Kropinski; Andre Villegas; Hans-Wolfgang Ackermann; Ana L. Toribio; Derek Pickard; Hany Anany; Todd Callaway; Andrew D. Brabban
Source:
Virology journal 2011 v.8 pp. 430-445
ISSN:
1743-422X
Subject:
genome; bacteriophages; Aeromonas; DNA; cytosine; Delftia; structural proteins; sequence analysis; feedlots; cattle; hosts; amino acid sequences; adhesion; Shigella; viral morphology; Salmonella Typhi; Pseudomonas phages; Vibrio phages; viral proteins; Bacillus subtilis; nucleotide sequences; Escherichia coli O157
Abstract:
... Phage vB_EcoM_CBA120 (CBA120), isolated against Escherichia coli O157:H7 from a cattle feedlot, is morphologically very similar to the classic phage ViI of Salmonella enterica serovar Typhi. Until recently, little was known genetically or physiologically about the ViI-like phages, and none targeting E. coli have been described in the literature. The genome of CBA120 has been fully sequenced and is ...
Handle:
10113/57319
DOI:
10.1186/1743-422X-8-430
PubMed:
21899740
PubMed Central:
PMC3184105

31. Characterization of bacteriophages with a lytic effect on various Salmonella serotypes and Escherichia coli O157:H7

Author:
Osvaldo López-Cuevas; Nohelia Castro-del Campo; Josefina León-Félix; Arturo González-Robles; Cristóbal Chaidez
Source:
Canadian journal of microbiology 2011 v.57 no.12 pp. 1042-1051
ISSN:
1480-3275
Subject:
DNA; Escherichia coli O157; Myoviridae; Salmonella Montevideo; Salmonella Typhimurium; bacteriophages; biological control; cattle; cell viability; feces; growth retardation; host range; polyacrylamide gel electrophoresis; poultry; proteins; restriction fragment length polymorphism; serotypes
Abstract:
... Four phages isolated from cattle and poultry feces were analyzed for their ability to lyse Salmonella serotypes and Escherichia coli O157:H7. The phage one-step growth curves, morphology, and genetic characteristics were determined. All phages showed a lytic effect on various Salmonella serotypes and E. coli O157:H7, which lysed at least 70% of the 234 strains tested. The phages had latent periods ...
DOI:
10.1139/w11-099

32. Chemical composition and antimicrobial effects of essential oils of Eucalyptus globulus, Myrtus communis and Satureja hortensis against Escherichia coli O157:H7 and Staphylococcus aureus in minced beef

Author:
D. Djenane; J. Yangüela; T. Amrouche; S. Boubrit; N. Boussad; P. Roncalés
Source:
Food science and technology international v.17 no.6 pp. 505-515
ISSN:
1082-0132
Subject:
Escherichia coli O157; Eucalyptus globulus; Myrtus communis; Satureja hortensis; Staphylococcus aureus; agar; antibacterial properties; beef; carvacrol; chemical composition; cineole; essential oils; gas chromatography; leaves; mass spectrometry; minced meat; minimum inhibitory concentration; odors; pathogens; sensory evaluation
Abstract:
... Essential oils (EOs) extracted by hydrodistillation from leaf parts of Algerian Eucalyptus globulus, Myrtus communis and Satureja hortensis were analyzed by gas chromatography/mass spectrometry (GC/MS). The main components of EOs obtained were γ-terpinene (94.48%), 1,8-cineole (46.98%) and carvacrol (46.10%), respectively, for E. globulus, M. communis and S. hortensis. The in vitro antimicrobial a ...
DOI:
10.1177/1082013211398803
PubMed:
22049156

33. Chicken feet bacteriological quality at 4 steps of technological processing

Author:
F. F. Santos; M. H. C. Aquino; E. R. Nascimento; D. L. C. Abreu; R. Gouvêa; D. P. Rodrigues; E. M. F. Reis; M. S. Araújo; V. L. A. Pereira
Source:
Poultry science 2011 v.90 no.12 pp. 2864-2868
ISSN:
0032-5791
Subject:
chickens; coliform bacteria; quantitative analysis; poultry production; serotypes; microbiological quality; Salmonella Anatum; foreign markets; Escherichia coli O157; qualitative analysis; feet
Abstract:
... The production of chicken feet is primarily intended for foreign markets, and there is still no specific legislation in Brazil that determines the quality standard of these products. The bacteriological quality of chicken feet was evaluated as a product for human consumption at different steps of the technological processes. Eighty broiler feet from 20 lots at 4 steps of processing were collected ...
DOI:
10.3382/ps.2011-01588
PubMed:
22080026

34. Chlorine Stabilizer T-128 Enhances Efficacy of Chlorine against Cross-Contamination by E. coli O157:H7 and Salmonella in Fresh-Cut Lettuce Processing

Author:
Nou, Xiangwu; Luo, Yaguang; Hollar, LaVonda; Yang, Yang; Feng, Hao; Millner, Patricia; Shelton, Daniel
Source:
Journal of food science: an official publication of the Institute of Food Technologists 2011 no.3 pp. M218
Subject:
shelf life; electrolytes; sanitizing; food packaging; Lactuca sativa var. capitata; carbon dioxide; bacterial contamination; headspace analysis; head lettuce; food storage; food quality; fresh-cut foods; oxygen; washing; chlorine; cutting; appearance (quality); storage time; cross contamination; plate count; stabilizers; antibacterial properties; sanitizers; Salmonella; food pathogens; Escherichia coli O157
Abstract:
... During fresh-cut produce processing, organic materials released from cut tissues can rapidly react with free chlorine in the wash solution, leading to the potential survival of foodborne bacterial pathogens, and cross-contamination when the free chlorine is depleted. A reported chlorine stabilizer, T-128, has been developed to address this problem. In this study, we evaluated the ability of T-128 ...
Handle:
10113/49554
DOI:
10.1111/j.1750-3841.2011.02046.x

35. Cloning of genes encoding colicin E2 in Lactococcus lactis subspecies lactis and evaluation of the colicin-producing transformants as inhibitors of Escherichia coli O157:H7 during milk fermentation

Author:
S.M. Liu; D.M. Miller; R.F. Roberts
Source:
Journal of dairy science 2011 v.94 no.3 pp. 1146-1154
ISSN:
0022-0302
Subject:
Escherichia coli O157; Lactococcus lactis subsp. lactis; assays; bacterial toxins; colicins; culture media; electroporation; fermentation; immunity; milk; molecular cloning; plasmid vectors; plasmids; secretion; starter cultures; structural genes
Abstract:
... Colicin E2 (ColE2) is a proteinaceous bacterial toxin produced by some strains of Escherichia coli and other members of the Enterobacteriaceae that exhibits inhibitory activity against some strains of E. coli O157:H7. A 2.0-kb DNA fragment, containing the ColE2 structural gene ceaB and immunity gene ceiB from E. coli NCTC 50133 (pColE2-P9), was cloned into the lactococcal plasmid vector pNZ2103. T ...
DOI:
10.3168/jds.2010-3539
PubMed:
21338780

36. Combination of bioprotective cultures with EDTA to reduce Escherichia coli O157:H7 in frozen ground-beef patties

Author:
Patricia Castellano; Carolina Belfiore; Graciela Vignolo
Source:
Food control 2011 v.22 no.8 pp. 1461-1465
ISSN:
0956-7135
Subject:
coliform bacteria; Lactobacillus curvatus; chelating agents; patties; ground beef; bacteriocins; EDTA (chelating agent); Lactococcus lactis; pH; color; population growth; Escherichia coli O157; temperature; pathogens
Abstract:
... The effectiveness of bacteriocin-producing Lactobacillus curvatus CRL705 and Lactococcus lactis CRL1109 in combination with Na₂EDTA on frozen ground-beef patties contaminated with Escherichia coli O157:H7, was investigated under temperature abuse conditions (5 °C during 9 days). The presence of the bioprotective cultures (ca. 10⁷ CFU/g) and chelator (48 mM) resulted in one log CFU/g reduction for ...
DOI:
10.1016/j.foodcont.2011.02.018

37. Combination treatment of alkaline electrolyzed water and citric acid with mild heat to ensure microbial safety, shelf-life and sensory quality of shredded carrots

Author:
S.M.E. Rahman; Yong-Guo Jin; Deog-Hwan Oh
Source:
Food microbiology 2011 v.28 no.3 pp. 484-491
ISSN:
0740-0020
Subject:
Escherichia coli O157; Listeria monocytogenes; acid treatment; bacteria; carrots; citric acid; dipping; exposure duration; food pathogens; heat; plate count; sensory properties; shelf life; sodium hypochlorite; spoilage; synergism; temperature; yeasts
Abstract:
... The objective of this study was to determine the synergistic effect of alkaline electrolyzed water and citric acid with mild heat against background and pathogenic microorganisms on carrots. Shredded carrots were inoculated with approximately 6–7 log CFU/g of Escherichia coli O157:H7 (932, and 933) and Listeria monocytogenes (ATCC 19116, and 19111) and then dip treated with alkaline electrolyzed w ...
DOI:
10.1016/j.fm.2010.10.006
PubMed:
21356455

38. Combined effect of reuterin and lactic acid bacteria bacteriocins on the inactivation of food-borne pathogens in milk

Author:
Juan L. Arqués; Eva Rodríguez; Manuel Nuñez; Margarita Medina
Source:
Food control 2011 v.22 no.3-4 pp. 457-461
ISSN:
0956-7135
Subject:
Aeromonas hydrophila; Campylobacter jejuni; Escherichia coli O157; Listeria monocytogenes; Salmonella enterica; Staphylococcus aureus; Yersinia enterocolitica; antibacterial properties; food pathogens; lactic acid bacteria; milk; nisin; synergism
Abstract:
... Antimicrobial activity of reuterin in combination with different bacteriocins from lactic acid bacteria against food-borne pathogens in milk was investigated. A strong synergistic effect of reuterin in combination with nisin, lacticin 481 or enterocin AS-48 on Listeria monocytogenes was observed. Only nisin increased the antimicrobial activity of reuterin against Staphylococcus aureus. Bactericida ...
DOI:
10.1016/j.foodcont.2010.09.027

39. Combined effect of ultrasound and organic acids to reduce Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes on organic fresh lettuce

Author:
Hun-Gu Sagong; Sun-Young Lee; Pahn-Shick Chang; Sunggi Heu; Sangryeol Ryu; Young-Jin Choi; Dong-Hyun Kang
Source:
International journal of food microbiology 2011 v.145 no.1 pp. 287-292
ISSN:
0168-1605
Subject:
leaf lettuce; texture; citric acid; Salmonella Typhimurium; ultrasonics; methodology; food pathogens; color; malic acid; Listeria monocytogenes; Escherichia coli O157; lactic acid
Abstract:
... This study was performed to compare the effectiveness of individual treatments (ultrasound and organic acids) and their combination on reducing foodborne pathogens on organic fresh lettuce. Lettuce leaves were inoculated with a cocktail of three strains each of Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes and treated with ultrasound (40kHz) alone, organic acids (0.3 ...
DOI:
10.1016/j.ijfoodmicro.2011.01.010
PubMed:
21276633

40. Combined effects of chlorine dioxide, drying, and dry heat treatments in inactivating microorganisms on radish seeds

Author:
Jihyun Bang; Haeyoung Kim; Hoikyung Kim; Larry R. Beuchat; Jee-Hoon Ryu
Source:
Food microbiology 2011 v.28 no.1 pp. 114-118
ISSN:
0740-0020
Subject:
Escherichia coli O157; air drying; bacteria; chlorine dioxide; heat; heat treatment; microbiological quality; pathogens; radishes; relative humidity; seeds; yeasts
Abstract:
... We determined the combined effectiveness of ClO₂ (200 and 500 μg/ml, 5 min), air drying [25 °C, 40% relative humidity (RH), 2 h], and mild dry heat (55 °C, 23% RH, up to 48 h) treatments in killing total aerobic bacteria (TAB), Escherichia coli O157:H7, and molds and yeasts (MY) on radish seeds. A 5.1-log reduction in the number of TAB was achieved on radish seeds treated with 200 or 500 μg/ml ClO ...
DOI:
10.1016/j.fm.2010.09.002
PubMed:
21056782

41. Combined effects of sodium chlorite dip treatment and chitosan coatings on the quality of fresh-cut d’Anjou pears

Author:
Zhenlei Xiao; Yangchao Luo; Yaguang Luo; Qin Wang
Source:
Postharvest biology and technology 2011 v.62 no.3 pp. 319-326
ISSN:
0925-5214
Subject:
Escherichia coli O157; antibacterial properties; catechol oxidase; chitosan; coatings; color; discoloration; edible films; enzymatic browning; enzyme activity; enzyme inhibition; firmness; food preservation; fresh-cut foods; fruit quality; fruits; pears; sodium chloride; vegetables; weight loss
Abstract:
... This study evaluated the effects of sodium chlorite (SC) alone and its sequential treatment with edible coatings on browning inhibition and quality maintenance of fresh-cut d’Anjou pears. Edible coatings were prepared from chitosan (CH) and its water-soluble derivative carboxymethyl chitosan (CMCH), separately. Pear wedges were immersed in SC solution, followed by coating with CH or CMCH solutions ...
Handle:
10113/54364
DOI:
10.1016/j.postharvbio.2011.07.007

42. Commensal Effect of Pectate Lyases Secreted from Dickeya dadantii on Proliferation of Escherichia coli O157:H7 EDL933 on Lettuce Leaves

Author:
Yamazaki, Akihiro; Li, Jin; Hutchins, William C.; Wang, Lixia; Ma, Jincai; Ibekwe, A. Mark; Yang, Ching-Hong
Source:
Applied and environmental microbiology 2011 v.77 no.1 pp. 156-162
ISSN:
0099-2240
Subject:
Escherichia coli O157; enteropathogens; enzyme activity; food safety; genes; humans; leaf lettuce; lyases; maceration; mutants; mutation; phyllosphere; plant pathogens; plant tissues; secretion; stress response; virulence
Abstract:
... The outbreaks caused by enterohemorrhagic Escherichia coli O157:H7 on leafy greens have raised serious and immediate food safety concerns. It has been suggested that several phytopathogens aid in the persistence and proliferation of the human enteropathogens in the phyllosphere. In this work, we examined the influence of virulence mechanisms of Dickeya dadantii 3937, a broad-host-range phytopathog ...
DOI:
10.1128/AEM.01079-10
PubMed:
21075884
PubMed Central:
PMC3019694

43. Comparative Evaluation of a Phage Protein Ligand Assay with Real-Time PCR and a Reference Method for the Detection of Escherichia coli O157:H7 in Raw Ground Beef and Trimmings

Author:
F. Savoye; P. Feng; C. Rozand; M. Bouvier; A. Gleizal; D. Thevenot
Source:
Journal of food protection 2011 v.74 no.1 pp. 6-12
ISSN:
0362-028X
Subject:
Escherichia coli O157; Food Safety and Inspection Service; bacteriophages; ground beef; ligands; livestock and meat industry; pathogens; quantitative polymerase chain reaction; rapid methods; raw meat; reverse transcriptase polymerase chain reaction
Abstract:
... Enterohemorrhagic Escherichia coli O157:H7 is an important pathogen associated with infections caused by consumption of undercooked raw meat. Sensitive and rapid detection methods for E. coli O157:H7 are essential for the meat industry to ensure a safe meat supply. This study was conducted to compare the sensitivity of the VIDAS ultraperformance E. coli test (ECPT UP) with a noncommercial real-tim ...
DOI:
10.4315/0362-028X.JFP-10-271

44. Comparison of Different Washing Treatments for Reducing Pathogens on Orange Surfaces and for Preventing the Transfer of Bacterial Pathogens to Fresh-Squeezed Orange Juice

Author:
N. E. Martinez-Gonzales; L. Martinez-Chavez; C. Martinez-Cardenas; A. Castillo
Source:
Journal of food protection 2011 v.74 no.10 pp. 1684-1691
ISSN:
0362-028X
Subject:
Escherichia coli O157; Listeria monocytogenes; Salmonella Typhimurium; chlorine; ethanol; lactic acid; orange juice; oranges; pH; pathogens; plate count; risk reduction; washing
Abstract:
... The objectives of this study were to compare the effectiveness of various washing treatments for reducing Escherichia coli O157:H7, Salmonella sp., and Listeria monocytogenes populations on orange surfaces and to measure the effect of some of these treatments in preventing the transfer of pathogens during juice extraction. Orange surfaces inoculated with L. monocytogenes or a mixture of E. coli O1 ...
DOI:
10.4315/0362-028X.JFP-10-357

45. Comparison of Two Possible Routes of Pathogen Contamination of Spinach Leaves in a Hydroponic Cultivation System

Author:
Shigenobu Koseki; Yasuko Mizuno; Kazutaka Yamamoto
Source:
Journal of food protection 2011 v.74 no.9 pp. 1536-1542
ISSN:
0362-028X
Subject:
Escherichia coli O157; Listeria monocytogenes; Salmonella; hydroponics; inoculum; leaves; odds ratio; pathogens; regression analysis; risk; root systems; roots; serotypes; spinach
Abstract:
... The route of pathogen contamination (from roots versus from leaves) of spinach leaves was investigated with a hydroponic cultivation system. Three major bacterial pathogens, Escherichia coli O157:H7, Salmonella, and Listeria monocytogenes, were inoculated into the hydroponic solution, in which the spinach was grown to give concentrations of 10(6) and 10(3) CFU/ml. In parallel, the pathogens were i ...
DOI:
10.4315/0362-028X.JFP-11-031

46. Cost of Illness and Disease Burden in The Netherlands Due to Infections with Shiga Toxin–Producing Escherichia coli O157

Author:
Luqman Tariq; Juanita Haagsma; Arie Havelaar
Source:
Journal of food protection 2011 v.74 no.4 pp. 545-552
ISSN:
0362-028X
Subject:
Escherichia coli O157; colitis; complications (disease); cost of illness analysis; diarrhea; disability-adjusted life year; food pathogens; gastroenteritis; health care costs; hemolytic uremic syndrome; simulation models; Netherlands
Abstract:
... Infections with Shiga toxin–producing Escherichia coli O157 (STEC O157) are associated with hemorrhagic colitis, hemolytic uremic syndrome (HUS), and end-stage renal disease (ESRD). In the present study, we extend previous estimates of the burden of disease associated with STEC O157 with estimates of the associated cost of illness in The Netherlands. A second-order stochastic simulation model was ...
DOI:
10.4315/0362-028X.JFP-10-252

47. Cross-Reactive Protection against Enterohemorrhagic Escherichia coli Infection by Enteropathogenic E. coli in a Mouse Model

Author:
Calderon Toledo, Carla; Arvidsson, Ida; Karpman, Diana
Source:
Infection and immunity 2011 v.79 no.6 pp. 2224-2233
ISSN:
0019-9567
Subject:
Escherichia coli O157; animal models; antibodies; antigens; blood serum; enteropathogenic Escherichia coli; genes; histology; histopathology; intimin; loci; mice; pathogenicity islands; pathogens; protective effect; weight loss
Abstract:
... Enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC) are related attaching and effacing (A/E) pathogens. The genes responsible for the A/E pathology are carried on a chromosomal pathogenicity island termed the locus of enterocyte effacement (LEE). Both pathogens share a high degree of homology in the LEE and additional O islands. EHEC prevalence is much lower in areas wher ...
DOI:
10.1128/IAI.01024-10
PubMed:
21402761
PubMed Central:
PMC3125830

48. Cross-protective effects of temperature, pH, and osmotic and starvation stresses in Escherichia coli O157:H7 subjected to pulsed electric fields in milk

Author:
Oscar Rodríguez-González; Markus Walkling-Ribeiro; Shesha Jayaram; Mansel W. Griffiths
Source:
International dairy journal 2011 v.21 no.12 pp. 953-962
ISSN:
0958-6946
Subject:
Escherichia coli O157; Weibull statistics; cold stress; heat; milk; models; pH; pulsed electric fields; sodium chloride; starvation; stress response; temperature
Abstract:
... The effect of exposure of Escherichia coli O157:H7 to prior stress on the effectiveness of pulsed electric field (PEF) treatment of milk was studied. Cells were exposed to a variety of temperatures (7–45 °C), pH (4.0–7.0), osmotic conditions (0–10% NaCl), starvation (100 h at 25 °C), and cold shock (5 °C for 250 h). Stress responses were explained by the Gompertz and Weibull models with similar go ...
DOI:
10.1016/j.idairyj.2011.07.003

49. Crystal Structure of the Passenger Domain of the Escherichia coli Autotransporter EspP

Author:
Shekeb Khan; Hira S. Mian; Linda E. Sandercock; Nickolay Y. Chirgadze; Emil F. Pai
Source:
Journal of molecular biology 2011 v.413 no.5 pp. 985-1000
ISSN:
0022-2836
Subject:
Escherichia coli O157; Gram-negative bacteria; crystal structure; pathogens; serine proteinases; virulence
Abstract:
... Autotransporters represent a large superfamily of known and putative virulence factors produced by Gram-negative bacteria. They consist of an N-terminal “passenger domain” responsible for the specific effector functions of the molecule and a C-terminal “β-domain” responsible for translocation of the passenger across the bacterial outer membrane. Here, we present the 2.5-Å crystal structure of the ...
DOI:
10.1016/j.jmb.2011.09.028
PubMed:
21964244
PubMed Central:
PMC3215816

50. CsgA Production by Escherichia coli O157:H7 Alters Attachment to Abiotic Surfaces in Some Growth Environments

Author:
R. M. Goulter-Thorsen; E. Taran; I. R. Gentle; K. S. Gobius; G. A. Dykes
Source:
Applied and environmental microbiology 2011 v.77 no.20 pp. 7339-7344
ISSN:
0099-2240
Subject:
Escherichia coli O157; adhesion; atomic force microscopy; attachment behavior; bacterial adhesion; contact angle; hydrocarbons; hydrophobicity; knockout mutants; stainless steel
Abstract:
... The role of curli expression in attachment of Escherichia coli O157:H7 to glass, Teflon, and stainless steel (SS) was investigated through the creation of csgA knockout mutants in two isolates of E. coli O157:H7. Attachment assays using epifluorescence microscopy and measurements of the force of adhesion of bacterial cells to the substrates using atomic force microscopy (AFM) force mapping were us ...
DOI:
10.1128/AEM.00277-11
PubMed:
21856839
PubMed Central:
PMC3194867

51. Dead-end ultrafiltration concentration and IMS/ATP-bioluminescence detection of Escherichia coli O157:H7 in recreational water and produce wash

Author:
Dawn M. Hunter; Stephaney D. Leskinen; Sonia Magaña; Sarah M. Schlemmer; Daniel V. Lim
Source:
Journal of microbiological methods 2011 v.87 no.3 pp. 338-342
ISSN:
0167-7012
Subject:
Escherichia coli O157; adenosine triphosphate; bioluminescence; disease outbreaks; fresh produce; microbial detection; pathogens; ultrafiltration; water pollution
Abstract:
... The purpose of this study was to develop a detection method for viable E. coli O157:H7 in fresh produce and recreational water. The method was evaluated using eight samples of produce wash and recreational water with or without spiked E. coli O157:H7 at ≤10²CFU·ml⁻¹ and concentrated using dead-end ultrafiltration (DEUF) to produce primary and secondary retentates. Fifty-four matrix replicates of u ...
DOI:
10.1016/j.mimet.2011.09.010

52. Defining treatment conditions for pulsed electric field pasteurization of apple juice

Author:
G. Saldaña; E. Puértolas; S. Monfort; J. Raso; I. Álvarez
Source:
International journal of food microbiology 2011 v.151 no.1 pp. 29-35
ISSN:
0168-1605
Subject:
electric field; Staphylococcus aureus; mathematical models; specific energy; Salmonella Typhimurium; pasteurization; apple juice; pulsed electric fields; Listeria monocytogenes; Escherichia coli O157; temperature; electrical resistance
Abstract:
... The influence of temperature and the presence of Nᵅ-lauroyl ethylester (ethyl lauroyl arginate, LAE) on the inactivation caused by continuous pulsed electric field treatments (PEF) in Escherichia coli O157:H7 suspended in apple juice have been investigated to define treatment conditions applicable at industrial scale that promote an equivalent safety level when compared with thermal processing. In ...
DOI:
10.1016/j.ijfoodmicro.2011.07.033
PubMed:
21880388

53. Detection of 5 CFU/g of Escherichia coli O157:H7 on lettuce using activated charcoal and real-time PCR without enrichment

Author:
Jung-Lim Lee; Robert E. Levin
Source:
Food microbiology 2011 v.28 no.3 pp. 562-567
ISSN:
0740-0020
Subject:
DNA; Escherichia coli O157; activated carbon; agitation; bentonite; cell suspension culture; charcoal; leaf lettuce; mixing; polymerase chain reaction; resins; sodium dodecyl sulfate
Abstract:
... A sample treatment method which separates Escherichia coli O157:H7 from lettuce and removes PCR inhibitors allowing 5 CFU/g of target cells to be detected using real-time PCR is described. Lettuce leaves inoculated with E. coli O157:H7 were rinsed with 0.025% sodium dodecyl sulfate (SDS). In this study, there were two major factors that strongly affected the recovery of E. coli O157:H7 during samp ...
DOI:
10.1016/j.fm.2010.11.007
PubMed:
21356465

54. Detection of E. coli O157:H7 by immunomagnetic separation coupled with fluorescence immunoassay

Author:
Peixuan Zhu; Daniel R. Shelton; Shuhong Li; Daniel L. Adams; Jeffrey S. Karns; Platte Amstutz; Cha-Mei Tang
Source:
Biosensors & bioelectronics 2011 v.30 no.1 pp. 337-341
ISSN:
0956-5663
Subject:
Escherichia coli O157; antibodies; binding capacity; biosensors; detection limit; enriched foods; fluorescence; immunoassays; immunomagnetic separation; quantitative polymerase chain reaction
Abstract:
... Conventional culture-based methods for detection of E. coli O157:H7 in foods and water sources are time-consuming, and results can be ambiguous, requiring further confirmation by biochemical testing and PCR. A rapid immunoassay prior to cultivation to identify presumptive positive sample would save considerable time and resources. Immunomagnetic separation (IMS) techniques are routinely used for i ...
DOI:
10.1016/j.bios.2011.09.029

55. Detection of E. coli O157:H7 in raw ground beef by Pathatrix™ immunomagnetic-separation, real-time PCR and cultural methods

Author:
Willis M. Fedio; Karen C. Jinneman; Ken J. Yoshitomi; Ruben Zapata; Chitra N. Wendakoon; Paul Browning; Stephen D. Weagant
Source:
International journal of food microbiology 2011 v.148 no.2 pp. 87-92
ISSN:
0168-1605
Subject:
Escherichia coli O157; flora; food matrix; ground beef; immunomagnetic separation; polymerase chain reaction
Abstract:
... Detection of Escherichia coli O157:H7 by conventional cultural methods can be difficult in food matrices with a high background flora such as raw ground beef. Raw ground beef samples, artificially contaminated separately with five strains of E. coli O157:H7 at low (~0.2cfu/g) and high (~2cfu/g) levels, were enriched by two enrichment protocols; buffered peptone water (BPW) at 37°C for 5h and 24h a ...
DOI:
10.1016/j.ijfoodmicro.2011.05.005
PubMed:
21641670

56. Detection of Escherichia coli O157:H7 virulence genes in isolates from beef, pork, water, human and animal species in the northwest province, South Africa: public health implications

Author:
Collins Njie Ateba; Moses Mbewe
Source:
Research in microbiology 2011 v.162 no.3 pp. 240-248
ISSN:
0923-2508
Subject:
Escherichia coli O157; Shiga toxin; beef; butcher shops; cattle; cities; farms; feces; genes; humans; pathogens; polymerase chain reaction; pork; public health; slaughterhouses; swine; virulence; South Africa
Abstract:
... The aim of the present study was to isolate and identify Escherichia coli O157:H7 from pigs, cattle, humans, beef, pork and water samples and to determine their putative virulence genes by PCR analysis. A total of 220 samples were analysed; 5600 presumptive E. coli O157:H7 were screened for the presence of rfbO₁₅₇ and fliCH₇ gene fragments by PCR and 130 isolates were confirmed. The prevalence of ...
DOI:
10.1016/j.resmic.2010.11.008

57. Detection of virulent Escherichia coli O157 strains using multiplex PCR and single base sequencing for SNP characterization

Author:
Haugum, K.; Brandal, L.T.; Løbersli, I.; Kapperud, G.; Lindstedt, B.-A.
Source:
Journal of applied microbiology 2011 v.110 no.6 pp. 1592-1600
ISSN:
1364-5072
Subject:
Escherichia coli O157; alleles; genotype; humans; minisatellite repeats; polymerase chain reaction; single nucleotide polymorphism; virulence; Norway
Abstract:
... Aims: To compare 167 Norwegian human and nonhuman Escherichia coli O157:H7/NM (nonmotile) isolates with respect to an A/T single nucleotide polymorphism (SNP) in the tir gene and to detect specific SNPs that differentiate STEC O157 into distinct virulence clades (1-3 and 8). Methods and Results: We developed a multiplex PCR followed by single base sequencing for detection of the SNPs, and examined ...
DOI:
10.1111/j.1365-2672.2011.05015.x

58. Determining Thermal Inactivation of Escherichia coli O157:H7 in Fresh Compost by Simulating Early Phases of the Composting Process

Author:
Singh, Randhir; Kim, Jinkyung; Shepherd, Marion W. Jr.; Luo, Feng; Jiang, Xiuping
Source:
Applied and environmental microbiology 2011 v.77 no.12 pp. 4126-4135
ISSN:
0099-2240
Subject:
Escherichia coli O157; Weibull statistics; carbon; composting; composts; dairies; heat inactivation; humidity; nitrogen; pathogen survival; pathogens; temperature
Abstract:
... A three-strain mixture of Escherichia coli O157:H7 was inoculated into fresh dairy compost (ca. 10⁷ CFU/g) with 40 or 50% moisture and was placed in an environmental chamber (ca. 70% humidity) that was programmed to ramp from room temperature to selected composting temperatures in 2 and 5 days to simulate the early composting phase. The surviving E. coli O157:H7 population was analyzed by direct p ...
DOI:
10.1128/AEM.02873-10
PubMed:
21498743
PubMed Central:
PMC3131655

59. Development of PCR assays for detection of Escherichia coli O157:H7 in meat products

Author:
Rubén Gordillo; Juan J. Córdoba; María J. Andrade; M. Isabel Luque; Mar Rodríguez
Source:
Meat science 2011 v.88 no.4 pp. 767-773
ISSN:
0309-1740
Subject:
DNA; Escherichia coli O157; bile; genes; immunomagnetic separation; polymerase chain reaction; pork; raw meat; screening
Abstract:
... A multiplex polymerase chain reaction (PCR) procedure based on fliCₕ₇ and rfbE genes was developed for the detection of Escherichia coli O157:H7 in raw pork meat and ready-to-eat (RTE) meat products. Two different DNA extraction procedures were evaluated for application on meat products. MasterPure™ DNA Purification kit in combination with immunomagnetic separation was found to be the best method ...
DOI:
10.1016/j.meatsci.2011.03.011
PubMed:
21458168

60. Development of a dynamic growth–death model for Escherichia coli O157:H7 in minimally processed leafy green vegetables

Author:
Robin C. McKellar; Pascal Delaquis
Source:
International journal of food microbiology 2011 v.151 no.1 pp. 7-14
ISSN:
0168-1605
Subject:
Escherichia coli O157; die-off; dynamic models; fresh produce; growth models; lettuce; pathogens; prediction; quantitative risk assessment; risk; root growth; shipping; spinach; temperature
Abstract:
... Escherichia coli O157:H7, an occasional contaminant of fresh produce, can present a serious health risk in minimally processed leafy green vegetables. A good predictive model is needed for Quantitative Risk Assessment (QRA) purposes, which adequately describes the growth or die-off of this pathogen under variable temperature conditions experienced during processing, storage and shipping. Literatur ...
DOI:
10.1016/j.ijfoodmicro.2011.07.027
PubMed:
21872959

61. Development of oscillation method for reducing foodborne pathogens on lettuce and spinach

Author:
Sung-Youn Kim; Hun-Gu Sagong; Sangryeol Ryu; Jae-Hyung Mah; Dong-Hyun Kang
Source:
International journal of food microbiology 2011 v.145 no.1 pp. 273-278
ISSN:
0168-1605
Subject:
leaf lettuce; spinach; Salmonella Typhimurium; food pathogens; Bacillus cereus; Listeria monocytogenes; Escherichia coli O157
Abstract:
... In this study, the efficacy of an oscillator for reducing the numbers of foodborne pathogens on lettuce and spinach was tested. A cocktail of three strains each of Salmonella typhimurium, Escherichia coli O157:H7 and Listeria monocytogenes cells and of Bacillus cereus spores was inoculated onto lettuce and spinach leaves and followed by oscillation at 10Hz and 20Hz for up to 30s. After treatment o ...
DOI:
10.1016/j.ijfoodmicro.2011.01.007
PubMed:
21269718

62. Dietary monensin level, supplemental urea, and ractopamine on fecal shedding of Escherichia coli O157:H7 in feedlot cattle

Author:
Z. D. Paddock; C. E. Walker; J. S. Drouillard; T. G. Nagaraja
Source:
Journal of animal science 2011 v.89 no.9 pp. 2829-2835
ISSN:
0021-8812
Subject:
diet; fermentation; antigens; feed additives; feedlots; hindgut; immunomagnetic separation; corn; feces; microbial ecology; monensin; steers; beef; finishing; urea; distillers grains; Escherichia coli O157; agglutination
Abstract:
... Inclusion of distillers grains (DG) in cattle diets has been shown to increase fecal shedding of Escherichia coli O157:H7. It is hypothesized that altered gut fermentation by DG may be responsible for the positive association. Therefore, feed additives affecting ruminal or hindgut fermentation of DG also may affect fecal shedding of E. coli O157:H7. The objectives of the study were to evaluate eff ...
DOI:
10.2527/jas.2010-3793
PubMed:
21512125

63. Different IS629 Transposition Frequencies Exhibited by Escherichia coli O157:H7 Strains in the Stepwise Evolutionary Model

Author:
Rump, Lydia V.; Fischer, Markus; González-Escalona, Narjol
Source:
Applied and environmental microbiology 2011 v.77 no.14 pp. 5030-5033
ISSN:
0099-2240
Subject:
Escherichia coli O157; models; transposons
Abstract:
... The insertion sequence IS629, which is highly prevalent in Escherichia coli O157:H7 genomes, was found to be absent in O157:H- strains, which are on a divergent pathway in the emergence of O157:H7. Although O157:H- is deficient in IS629, it permits IS629 transposition, with an excision frequency higher than that of ancestral O55:H7 strains but significantly lower than that of pathogenic O157:H7 st ...
DOI:
10.1128/AEM.00249-11
PubMed:
21622790
PubMed Central:
PMC3147399

64. Differential Gene Expression of E. coli O157:H7 in Ground Beef Extract Compared to Tryptic Soy Broth

Author:
Pina M. Fratamico; Siyum Wang; Xianghe Yan; Wei Zhang; Yuesheng Li
Source:
Journal of food science 2011 v.76 no.1 pp. M79
ISSN:
1750-3841
Subject:
Escherichia coli O157; RNA; amino acids; bacteria; bacterial contamination; biosynthesis; complementary DNA; enzymes; food contamination; food matrix; gene expression; gene expression regulation; genes; ground beef; microarray technology; oligonucleotide probes; open reading frames; plasmids; protein degradation; protein synthesis; reverse transcriptase polymerase chain reaction; reverse transcription; transcription factors; transporters
Abstract:
... Escherichia coli O157:H7 causes sporadic cases and outbreaks of hemorrhagic colitis and hemolytic uremic syndrome, and ground beef is a common vehicle of infection. DNA microarrays have been used for transcriptomic studies of E. coli O157:H7 using laboratory media; however, analysis of gene expression in complex matrices such as food are lacking. This study compared gene expression profiles of E. ...
Handle:
10113/56094
DOI:
10.1111/j.1750-3841.2010.01952x

65. Differential expression of genes in the earthworm Eisenia fetida following exposure to Escherichia coli O157:H7

Author:
Xing Wang; Li Chang; Zhenjun Sun
Source:
Developmental and comparative immunology 2011 v.35 no.5 pp. 525-529
ISSN:
0145-305X
Subject:
ferritin; Eisenia fetida; H+/K+-exchanging ATPase; polluted soils; immunity; clones; cytoskeleton; gene expression; cleaning; gene expression regulation; metabolism; H-transporting ATP synthase; complementary DNA; amino acid sequences; cDNA libraries; earthworms; suppression subtractive hybridization; lysozyme; quantitative polymerase chain reaction; translation (genetics); proteinases; expressed sequence tags; myosin; Escherichia coli O157
Abstract:
... In this study, suppression subtractive hybridization was used to construct forward and reverse cDNA libraries to identify genes involved in the response of Eisenia fetida after exposure to Escherichia coli O157:H7. We cloned 1428 cDNAs or expressed sequence tags (ESTs), of which 738 were confirmed to be differentially expressed on dot blotting analysis. A total of 394 good-quality ESTs (GenBank db ...
DOI:
10.1016/j.dci.2010.12.014

66. Direct and sensitive detection of foodborne pathogens within fresh produce samples using a field-deployable handheld device

Author:
David J. You; Kenneth J. Geshell; Jeong-Yeol Yoon
Source:
Biosensors & bioelectronics 2011 v.28 no.1 pp. 399-406
ISSN:
0956-5663
Subject:
Escherichia coli K12; Escherichia coli O157; Lactuca sativa var. capitata; antibodies; antigens; biosensors; cost effectiveness; cross reaction; detection limit; food pathogens; fresh produce; grinding; latex; particle size; plant tissues; washing; wavelengths
Abstract:
... Direct and sensitive detection of foodborne pathogens from fresh produce samples was accomplished using a handheld lab-on-a-chip device, requiring little to no sample processing and enrichment steps for a near-real-time detection and truly field-deployable device. The detection of Escherichia coli K12 and O157:H7 in iceberg lettuce was achieved utilizing optimized Mie light scatter parameters with ...
DOI:
10.1016/j.bios.2011.07.055

67. Disinfection performances of stored acidic and neutral electrolyzed waters generated from brine solution

Author:
Grace M. Nisola; Xiaohong Yang; Eulsaeng Cho; Midoek Han; Changhee Lee; Wook-Jin Chung
Source:
Journal of environmental science and health 2011 v.46 no.3 pp. 263-270
ISSN:
1532-4117
Subject:
Escherichia coli O157; Salmonella; chlorine; disinfection; pH; storage conditions; volatilization
Abstract:
... The feasibility of storing two electrolyzed waters (EW), acidic (AEW) and neutral (NEW), were elucidated through Escherichia coli O157:H7 and Salmonella typhii inactivation experiments. Free chlorine (FC) loss, pH and oxidation-reduction potentials were monitored for 30 days. Initial activities of fresh EWs were determined at 5 mg Cl₂·min/L for 8 Log₁₀ inactivations of both strains. However, store ...
DOI:
10.1080/10934529.2011.535428
PubMed:
21308597

68. Distinct Acid Resistance and Survival Fitness Displayed by Curli Variants of Enterohemorrhagic Escherichia coli O157:H7

Author:
Carter, Michelle Q.; Brandl, Maria T.; Louie, Jacqueline W.; Kyle, Jennifer L.; Carychao, Diana K.; Cooley, Michael B.; Parker, Craig T.; Bates, Anne H.; Mandrell, Robert E.
Source:
Applied and environmental microbiology 2011 v.77 no.11 pp. 3685-3695
ISSN:
0099-2240
Subject:
Escherichia coli O157; acid tolerance; biofilm; fimbriae; hamburgers; hosts; humans; mutants; oxygen; spinach; starvation; stomach; United States
Abstract:
... Curli are adhesive fimbriae of Enterobacteriaceae and are involved in surface attachment, cell aggregation, and biofilm formation. Here, we report that both inter- and intrastrain variations in curli production are widespread in enterohemorrhagic Escherichia coli O157:H7. The relative proportions of curli-producing variants (C⁺) and curli-deficient variants (C⁻) in an E. coli O157:H7 cell populati ...
DOI:
10.1128/AEM.02315-10
PubMed:
21478320
PubMed Central:
PMC3127601

69. Do leafy green vegetables and their ready-to-eat [RTE] salads carry a risk of foodborne pathogens?

Author:
Birce Mercanoglu Taban; A. Kadir Halkman
Source:
Anaerobe 2011 v.17 no.6 pp. 286-287
ISSN:
1075-9964
Subject:
Escherichia coli O157; Listeria monocytogenes; Salmonella; children; eating habits; elderly; farm to fork; food pathogens; foodborne illness; harvesting; humans; ingestion; international trade; irrigation water; lettuce; lifestyle; monitoring; organic fertilizers; packaging; poisoning; pregnant women; ready-to-eat foods; restaurants; risk; salads; wildlife; Europe; United States
Abstract:
... Over the past 10 years, there is an increasing demand for leafy green vegetables and their ready-to-eat (RTE) salads since people changed their eating habits because of healthier lifestyle interest. Nevertheless fresh leafy green vegetables and their RTE salads are recognized as a source of food poisoning outbreaks in many parts of the world. However, this increased proportion of outbreaks cannot ...
DOI:
10.1016/j.anaerobe.2011.04.004

70. Effect of Repeated Irrigation with Water Containing Varying Levels of Total Organic Carbon on the Persistence of Escherichia coli O157:H7 on Baby Spinach

Author:
David T. Ingram; Jitu Patel; Manan Sharma
Source:
Journal of food protection 2011 v.74 no.5 pp. 709-717
ISSN:
0362-028X
Subject:
carbon; irrigation scheduling; bacterial contamination; cell viability; spinach; marketing; water pollution; water quality; aerial parts; lettuce; spraying; plant tissues; irrigation water; cattle manure; Spinacia oleracea; epiphytes; foliar application; Escherichia coli O157; growth chambers; California
Abstract:
... The California lettuce and leafy greens industry has adopted the Leafy Greens Marketing Agreement (LGMA), which allows for 126 most-probable-number (MPN) Escherichia coli per 100 ml in irrigation water. Repeat irrigation of baby spinach plants with water containing E. coli O157:H7 and different levels of total organic carbon (TOC) was used to determine the epiphytic survival of E. coli O157:H7. Th ...
Handle:
10113/53796
DOI:
10.4315/0362-028X.JFP-10-426
PubMed:
21549040

71. Effect of UV-C light on anthocyanin content and other quality parameters of pomegranate juice

Author:
Çiğdem Uysal Pala; Ayşegül Kırca Toklucu
Source:
Journal of food composition and analysis 2011 v.24 no.6 pp. 790-795
ISSN:
0889-1575
Subject:
Escherichia coli O157; anthocyanins; antioxidant activity; color; heat treatment; irradiation; juice quality; juices; phenol; plate count; pomegranates; ultraviolet radiation; yeasts
Abstract:
... Pomegranate juice (PJ) was subjected to UV-C irradiation as a non-thermal technology and changes in major quality characteristics of juice such as anthocyanins, polymeric colour, antioxidant activity and total phenol content were determined. The results were compared with control (untreated) and heat treated (at 90°C, 2min) juice samples. UV-C treatment preserved the major quality characteristics ...
DOI:
10.1016/j.jfca.2011.01.003

72. Effect of a New Probiotic Saccharomyces cerevisiae Strain on Survival of Escherichia coli O157:H7 in a Dynamic Gastrointestinal Model

Author:
Etienne-Mesmin, Lucie; Livrelli, Valérie; Privat, Maud; Denis, Sylvain; Cardot, Jean-Michel; Alric, Monique; Blanquet-Diot, Stéphanie
Source:
Applied and environmental microbiology 2011 v.77 no.3 pp. 1127-1131
ISSN:
0099-2240
Subject:
Escherichia coli O157; Saccharomyces cerevisiae; bacteria; duodenum; dynamic models; ethanol production; mortality; probiotics; stomach
Abstract:
... Survival of Escherichia coli O157:H7 was investigated using a dynamic gastrointestinal model. A high bacterial mortality was observed in the stomach and duodenum. In contrast, bacteria grew in the distal parts of the small intestine. The coadministration of Saccharomyces cerevisiae CNCM I-3856 led to a significant reduction of bacterial resumption, maybe through ethanol production. ...
DOI:
10.1128/AEM.02130-10
PubMed:
21131521
PubMed Central:
PMC3028742

73. Effect of a nutritional reconditioning program for thin dairy cattle on body weight, carcass quality, and fecal pathogen shedding

Author:
Maier, Gabriele U.; Hoar, Bruce R.; Stull, Carolyn L.; Kass, Philip H.; Villanueva, Veronica; Maas, John
Source:
Journal of the American Veterinary Medical Association 2011 v.239 no.12 pp. 1594-1602
ISSN:
0003-1488
Subject:
Bovine leukemia virus; Escherichia coli O157; Holstein; USDA; adults; antibodies; average daily gain; blood; body condition; body weight changes; carcass quality; carcass weight; clinical trials; dairy cows; dressing percentage; feces; human resources; lymphoma; marbling; markets; meat cuts; pathogens; seroprevalence; slaughter
Abstract:
... Objective-To assess changes in body weight, carcass quality, and fecal pathogen shedding in cull dairy cows fed a high-energy ration for 28 or 56 days prior to slaughter. Design-Randomized clinical trial. Animals-31 adult Holstein dairy cows. Procedures-Cows were randomly assigned to a control (immediate slaughter) group or a 28-day or 56-day feeding group. Cows in the feeding groups received a hi ...
DOI:
10.2460/javma.239.12.1594

74. Effect of curli expression and hydrophobicity of Escherichia coli O157:H7 on attachment to fresh produce surfaces

Author:
Patel, J.; Sharma, M.; Ravishakar, S.
Source:
Journal of applied microbiology 2011 v.110 no.3 pp. 737-745
ISSN:
1364-5072
Subject:
romaine lettuce; bacterial contamination; food surfaces; biofilm; adhesins; Escherichia coli O157; food contamination; bacterial adhesion; cabbage; cross contamination; Lactuca sativa var. capitata; hydrophobicity; fresh-cut foods
Abstract:
... Aim: To investigate the effect of curli expression on cell hydrophobicity, biofilm formation and attachment to cut and intact fresh produce surfaces. Methods and Results: Five Escherichia coli O157:H7 strains were evaluated for curli expression, hydrophobicity, biofilm formation and attachment to intact and cut fresh produce (cabbage, iceberg lettuce and Romaine lettuce) leaves. Biofilm formation ...
Handle:
10113/50453
DOI:
10.1111/j.1365-2672.2010.04933.x

75. Effect of juice processing on cranberry antibacterial properties

Author:
J. Côté; S. Caillet; D. Dussault; J.-F. Sylvain; M. Lacroix
Source:
Food research international 2011 v.44 no.9 pp. 2922-2929
ISSN:
0963-9969
Subject:
processing technology; Pseudomonas aeruginosa; Staphylococcus aureus; mash; pomace; minimum inhibitory concentration; Enterococcus faecium; Salmonella Typhimurium; phenol; juice concentrates; cranberries; Listeria monocytogenes; Escherichia coli O157; antibacterial properties
Abstract:
... The effects of the industrial juice process on the ability of neutralized cranberry samples and extracts (polar, apolar and anthocyanins) to inhibit the growth of Enterococcus faecium resistant to vancomycin (ERV), Escherichia coli O157:H7 EDL 933, E. coli ATCC 25922, Listeria monocytogenes HPB2812, Pseudomonas aeruginosa ATCC 15442, Salmonella Typhimurium SL1344 and Staphylococcus aureus ATCC 292 ...
DOI:
10.1016/j.foodres.2011.06.049

76. Effect of modified atmosphere packaging on the persistence and expression of virulence factors of Escherichia coli O157:H7 on shredded iceberg lettuce

Author:
Sharma, Manan; Lakshman, Sudesna; Ferguson, Sean; Ingram, David T.; Luo, Yaguang; Patel, Jitu
Source:
Journal of food protection 2011 v.74 no.5 pp. 718-726
ISSN:
0362-028X
Subject:
shelf life; food packaging; carbon dioxide; bacterial contamination; heat tolerance; head lettuce; modified atmosphere packaging; food storage; storage temperature; ambient temperature; fresh-cut foods; virulence; oxygen; films (materials); packaging materials; storage time; food safety; stress tolerance; nitrogen; shredding; food pathogens; Escherichia coli O157; permeability
Abstract:
... Fresh-cut leafy greens contaminated with Escherichia coli O157:H7 have caused foodborne outbreaks. Packaging conditions, coupled with abusive storage temperatures of contaminated lettuce, were evaluated for their effect on the potential virulence of E. coli O157:H7. Shredded lettuce was inoculated with 5.58 and 3.98 log CFU E. coli O157:H7 per g and stored at 4 and 15°C, respectively, for up to 10 ...
Handle:
10113/49560
DOI:
10.4315/0362-028X.JFP-10-427

77. Effect of organic acids, hydrogen peroxide and mild heat on inactivation of Escherichia coli O157:H7 on baby spinach

Author:
Yaoxin Huang; Haiqiang Chen
Source:
Food control 2011 v.22 no.8 pp. 1178-1183
ISSN:
0956-7135
Subject:
Escherichia coli O157; acetic acid; chlorine; citric acid; foodborne illness; heat; heat inactivation; hydrogen peroxide; lactic acid; leaves; malic acid; population dynamics; spinach; tartaric acid; washing
Abstract:
... Minimally processed baby spinach contaminated with Escherichia coli O157:H7 has been associated with multiple outbreaks of foodborne illnesses recently. Chlorinated water is widely used to wash vegetables commercially, but this washing procedure has limited efficacy and can lead to the formation of carcinogenic substances. This study was conducted to determine the effects of organic acids and hydr ...
DOI:
10.1016/j.foodcont.2011.01.012

78. Effect of rapeseed protein-gelatin film containing grapefruit seed extract on ‘Maehyang' strawberry quality

Author:
Jang, Sung-Ae; Shin, Yoon-Ji; Song, Kyung Bin
Source:
International journal of food science & technology 2011 v.46 no.3 pp. 620-625
ISSN:
0950-5423
Subject:
Escherichia coli O157; Listeria monocytogenes; bacteria; edible films; grapefruits; population dynamics; rapeseed; seed extracts; sensory evaluation; shelf life; strawberries; yeasts
Abstract:
... To develop a packaging film for ‘Maehyang' strawberries, an edible film containing antimicrobial grapefruit seed extract (GSE) was manufactured. Addition of GSE to the rapeseed protein-gelatin (RG) film inhibited the growth of pathogenic bacteria such as Escherichia coli O157:H7 and Listeria monocytogenes. Packaging of ‘Maehyang' strawberries with the RG film containing 1.0% GSE decreased the popu ...
DOI:
10.1111/j.1365-2621.2010.02530.x

79. Effects of Long- or Short-Haul Transportation of Slaughter Heifers and Cattle Liner Microclimate on Hide Contamination with Escherichia coli O157

Author:
K. Stanford; M. Bryan; J. Peters; L. A. Gonzalez; T. P. Stephens; K. S. Schwartzkopf-Genswein
Source:
Journal of food protection 2011 v.74 no.10 pp. 1605-1610
ISSN:
0362-028X
Subject:
Angus; Escherichia coli O157; ambient temperature; animal transport; body weight; cross contamination; crossbreds; feedlots; heifers; ice; immunomagnetic separation; microclimate; microprocessors; nose; perineum; relative humidity; slaughter; slaughterhouses; trailers; transportation; Alberta
Abstract:
... Effects of cattle transportation on hide contamination with Escherichia coli O157 have been variable, and the present study was designed to clarify relationships among duration of transport, microclimate, and environment within the trailer and contamination of hides with E. coli O157. Crossbred Angus heifers from a feedlot in southern Alberta were sorted into 10 replicate loads containing 45 anima ...
DOI:
10.4315/0362-028X.JFP-11-154

80. Effects of Salt, Sodium Pyrophosphate, and Sodium Lactate on the Probability of Growth of Escherichia coli O157:H7 in Ground Beef

Author:
Chen-An Hwang; Vijay Juneja
Source:
Journal of food protection 2011 v.74 no.4 pp. 622-626
ISSN:
0362-028X
Subject:
Escherichia coli O157; additives; ground beef; lactic acid; models; probability; regression analysis; sodium; sodium pyrophosphate; temperature; vacuum packaging
Abstract:
... Ground beef products are susceptible to contamination with Escherichia coli O157:H7. The objective of this study was to examine the effect of salt, sodium pyrophosphate (SPP), and sodium lactate on the probability of growth of E. coli O157:H7 in ground beef under a temperature abuse condition. Ground beef containing 0 to 2.25% salt, 0 to 0.5% SPP, and 0 to 3% lactate was inoculated with a four-str ...
DOI:
10.4315/0362-028X.JFP-10-325

81. Effects of Simulated Dry and Wet Chilling and Aging of Beef Fat and Lean Tissues on the Reduction of Escherichia coli O157:H7 and Salmonella

Author:
A. W. Tittor; M. G. Tittor; M. M. Brashears; J. C. Brooks; A. J. Garmyn; M. F. Miller
Source:
Journal of food protection 2011 v.74 no.2 pp. 289-293
ISSN:
0362-028X
Subject:
Escherichia coli O157; Salmonella; air; beef; cold treatment; critical control points; microorganisms; plate count; refrigeration; relative humidity; tallow; tissues; vacuum packaging
Abstract:
... The efficacy of dry and wet chilling and aging of beef as methods for the reduction of Escherichia coli O157:H7 and Salmonella on lean and fat tissues was studied. Samples were obtained from a harvest facility prior to antimicrobial interventions and were inoculated with a cocktail mixture of E. coli O157:H7 or Salmonella to achieve a target inoculation of 6 log CFU/cm2. Wet chilled and aged sampl ...
DOI:
10.4315/0362-028X.JFP-10-295

82. Effects of Sodium Citrate plus Sodium Diacetate and Buffered Vinegar on Escherichia coli O157:H7 and Psychrotrophic Bacteria in Brine-Injected Beef

Author:
Amudhan Ponrajan; Mark A. Harrison; Jacob R. Segers; Bradley K. Lowe; Russell O. McKeith; T. Dean Pringle; Karina G. Martino; Jake H. Mulligan; Alexander M. Stelzleni
Source:
Journal of food protection 2011 v.74 no.3 pp. 359-364
ISSN:
0362-028X
Subject:
Escherichia coli O157; beef; brine solutions; internal temperature; muscles; psychrotrophic bacteria; shelf life; sodium; sodium chloride; sodium citrate; sodium tripolyphosphate; steaks; storage time; vacuum packaging; vinegars
Abstract:
... The objective of this research was to examine the effects of sodium citrate plus sodium diacetate or buffered vinegar on Escherichia coli O157:H7 and psychrotrophic bacteria when incorporated in brine solutions for injected beef. Two experiments were conducted in which 30 top rounds and 30 top sirloins were injected (110%) to contain (i) 0.5% sodium chloride and 0.4% sodium tripolyphosphate as the ...
DOI:
10.4315/0362-028X.JFP-10-294

83. Effects of Vacuum or Modified Atmosphere Packaging in Combination with Irradiation for Control of Escherichia coli O157:H7 in Ground Beef Patties

Author:
Li L. Kudra; Joseph G. Sebranek; James S. Dickson; Aubrey F. Mendonca; Elaine M. Larson; Armitra L. Jackson-Davis; Zheng Lu
Source:
Journal of food protection 2011 v.74 no.12 pp. 2018-2023
ISSN:
0362-028X
Subject:
Escherichia coli O157; carbon dioxide; cold storage; ground beef; irradiation; modified atmosphere packaging; pathogens; patties; temperature; vacuum packaging
Abstract:
... The efficacy of controlling Escherichia coli O157:H7 in ground beef patties by combining irradiation with vacuum packaging or modified atmosphere packaging (MAP) was investigated. Fresh ground beef patties were inoculated with a five-strain cocktail of E. coli O157:H7 at 5 log CFU/g. Single patties, packaged with vacuum or high-CO2 MAP (99.6% CO2 plus 0.4% CO), were irradiated at 0 (control), 0.5, ...
DOI:
10.4315/0362-028X.JFP-11-289

84. Effects of culture conditions on the subsequent heat inactivation of E. coli O157:H7 in apple juice

Author:
Alonzo A. Gabriel; Hiroyuki Nakano
Source:
Food control 2011 v.22 no.8 pp. 1456-1460
ISSN:
0956-7135
Subject:
Escherichia coli O157; apple juice; citric acid; environmental factors; glucose; heat; heat inactivation; pH
Abstract:
... Escherichia coli O157:H7 cells were grown in various acidic growth media including an acidogenic nutrient broth (NB) supplemented with 1% glucose (NBG) and NB acidified to pH 4.5 with 0.25% citric or malic acids. The pH in NBG continuously decreased, reaching a final pH of 4.46 after 28 h. The pH in the control culture (NB) did not significantly change throughout the incubation. When heated in app ...
DOI:
10.1016/j.foodcont.2011.03.011

85. Effects of sodium lactate on the survival of Listeria monocytogenes, escherichia coli 0157:H7 and Salmonella spp., in cooked ham at refrigeration and abuse temperatures

Author:
Cheng-An Hwang; Shiowshuh Sheen; Vijay Juneja
Source:
Food and nutrition sciences 2011 v.2 no.5 pp. 464-470
ISSN:
2157-9458
Subject:
Escherichia coli O157; Listeria monocytogenes; Salmonella; cooked foods; food safety; ham; lactates; microbial growth; microbiological quality; pathogens; ready-to-eat foods; refrigeration; sodium; storage temperature
Abstract:
... The objective of this study was to determine the effect of sodium lactate on the survival of Listeria monocytogenes, Escherichia coli O157:H7 and Salmonella spp. in cooked ham during storage at refrigeration and abuse temperatures. Cooked ham was added with 0-3% lactate, inoculated with a multiple-strain cocktail of L. monocytogenes, E. coli O157:H7 or Salmonella spp. and stored at 4-15 degree C f ...
Handle:
10113/56663
DOI:
10.4236/fns.2011.25066

86. Effects of sugar addition in Luria Bertani (LB) media on Escherichia coli O157:H7

Author:
Medina, Marjorie B.; Uknalis, Joseph; Tu, Shu-I
Source:
Journal of food safety 2011 v.31 no.3 pp. 386-394
ISSN:
0149-6085
Subject:
Escherichia coli O157; Escherichia infections; Shiga toxin; Shiga-like toxins; bacteria; cell growth; chemistry; foods; glucose; hemolytic uremic syndrome; humans; lactose; metabolism; pathogenicity; quorum sensing
Abstract:
... Human pathogenic Escherichia coli O157:H7 produces Shiga-like toxins (SLT) that cause hemolytic uremic syndrome. Typically, SLT are released when a bacterium lyses, but the mechanism of controlling SLT production is not clearly understood. This paper studies the cell growth and metabolism of E. coli in Luria Bertani (LB) broth with and without added sugars. Sugars added to LB broth cause a variety ...
DOI:
10.1111/j.1745-4565.2011.00311.x

87. Efficacy of Levulinic Acid–Sodium Dodecyl Sulfate against Encephalitozoon intestinalis, Escherichia coli O157:H7, and Cryptosporidium parvum

Author:
Ynes R. Ortega; Maria P. Torres; Jessica M. Tatum
Source:
Journal of food protection 2011 v.74 no.1 pp. 140-144
ISSN:
0362-028X
Subject:
Cryptosporidium parvum; Encephalitozoon intestinalis; Escherichia coli O157; bacteria; calves; exposure duration; feces; food industry; humans; in vitro culture; levulinic acid; meat; oocysts; parasites; pathogenicity; sanitizers; sodium dodecyl sulfate; spores; sulfates; viability; Oregon
Abstract:
... Foodborne parasites are characterized as being highly resistant to sanitizers used by the food industry. In 2009, a study reported the effectiveness of levulinic acid in combination with sodium dodecyl sulfate (SDS) in killing foodborne bacteria. Because of their innocuous properties, we studied the effects of levulinic acid and SDS at various concentrations appropriate for use in foods, on the vi ...
DOI:
10.4315/0362-028X.JFP-10-104

88. Efficacy of adding detergents to sanitizer solutions for inactivation of Escherichia coli O157:H7 on Romaine lettuce

Author:
Lindsey A. Keskinen; Bassam A. Annous
Source:
International journal of food microbiology 2011 v.147 no.3 pp. 157-161
ISSN:
0168-1605
Subject:
Escherichia coli O157; buffers; chlorine; chlorine dioxide; detergents; leaf lettuce; leaves; mixing; neutralization; romaine lettuce; sanitizers; selective media; short chain fatty acids; sodium; sulfates; washing
Abstract:
... Numerous Escherichia coli O157:H7 outbreaks have been linked to consumption of fresh lettuce. The development of effective and easily implemented wash treatment could reduce such incidents. The purpose of this study was to evaluate the addition of food-grade detergents to sanitizer solutions for inactivation of E. coli O157:H7 on Romaine lettuce. Freshly-cut leaves of Romaine lettuce were dip-inoc ...
DOI:
10.1016/j.ijfoodmicro.2011.04.002
PubMed:
21529976

89. Efficacy of various plant hydrosols as natural food sanitizers in reducing Escherichia coli O157:H7 and Salmonella Typhimurium on fresh cut carrots and apples

Author:
Fatih Tornuk; Hasan Cankurt; Ismet Ozturk; Osman Sagdic; Okan Bayram; Hasan Yetim
Source:
International journal of food microbiology 2011 v.148 no.1 pp. 30-35
ISSN:
0168-1605
Subject:
apples; fruits; sage; sanitizers; Salmonella Typhimurium; thyme; tap water; washing; rosemary; Nigella sativa; Escherichia coli O157; natural foods; carrots; leaves; antibacterial properties
Abstract:
... In the present study, inhibitory effects of the hydrosols of thyme, black cumin, sage, rosemary and bay leaf were investigated against Salmonella Typhimurium and Escherichia coli O157:H7 inoculated to apple and carrots (at the ratio of 5.81 and 5.81 log cfu/g for S. Typhimurium, and 5.90 and 5.70 log cfu/g for E. coli O157:H7 on to apple and carrot, respectively). After the inoculation of S. Typhi ...
DOI:
10.1016/j.ijfoodmicro.2011.04.022
PubMed:
21592604

90. Efficacy of washing meat surfaces with 2% levulinic, acetic, or lactic acid for pathogen decontamination and residual growth inhibition

Author:
C.E. Carpenter; J.V. Smith; J.R. Broadbent
Source:
Meat science 2011 v.88 no.2 pp. 256-260
ISSN:
0309-1740
Subject:
Escherichia coli O157; Listeria monocytogenes; Salmonella; acetic acid; bacteria; beef; chicken skin; decontamination; lactic acid; levulinic acid; pathogens; pork bellies; turkeys; washing
Abstract:
... We compared spray washing at 55.4°C with 2% levulinic acid to that with lactic or acetic acid for decontamination of pathogenic bacteria inoculated onto meat surfaces, and their residual protection against later growth of pathogenic bacteria. The model systems included Escherichia coli O157:H7 on beef plate, Salmonella on chicken skin and pork belly, and Listeria monocytogenes on turkey roll. In t ...
DOI:
10.1016/j.meatsci.2010.12.032
PubMed:
21251765

91. Enhanced immunogenicity of a novel Stx2Am-Stx1B fusion protein in a mice model of enterohemorrhagic Escherichia coli O157:H7 infection

Author:
Kun Cai; Xiang Gao; Tao Li; Qin Wang; Xiaojun Hou; Wei Tu; Le Xiao; Maoren Tian; Yuenan Liu; Hui Wang
Source:
Vaccine 2011 v.29 no.5 pp. 946-952
ISSN:
0264-410X
Subject:
Escherichia coli O157; cytokines; hemolytic uremic syndrome; humans; immune response; immunoglobulin G; interferon-gamma; lethal dose; mice; models; neutralizing antibodies; vaccines; verotoxins
Abstract:
... Shiga toxins (Stxs) which include Stx1 and Stx2 produced by EHEC O157:H7 are responsible for severe diseases, including hemolytic uremic syndrome (HUS) in humans. In our previous study, a fusion protein Stx2B-Stx1B (2S for short) was prepared and displayed immunogenicity against low lethal dose challenge of E. coli O157:H7. To enhance the immunogenicity against both toxins above, we constructed a ...
DOI:
10.1016/j.vaccine.2010.11.035

92. Enhancement of antimicrobial activities of naturally occurring phenolic compounds by nanoscale delivery against listeria monocytogenes, escherichia coli o157:h7 and salmonella typhimurium in broth and chicken meat system

Author:
MADHURAM RAVICHANDRAN; NAVAM S. HETTIARACHCHY; VIJAYALAKSHMI GANESH; STEVEN C. RICKE; SURENDRA SINGH
Source:
Journal of food safety 2011 v.31 no.4 pp. 462-471
ISSN:
0149-6085
Subject:
antimicrobial properties; vegetables; tea; cooked foods; fruits; chicken meat; benzoic acid; Salmonella Typhimurium; disinfectants; encapsulation; food pathogens; Listeria monocytogenes; Escherichia coli O157; anti-infective agents; food safety; nanoparticles; phenolic compounds
Abstract:
... Phenolic compounds commonly occurring in fruits, vegetables and tea were studied for their effects on Listeria monocytogenes (L.m.), Escherichia coli O157:H7 (E.c.) and Salmonella Typhimurium (S.T.) in brain–heart infusion broth (BHI) and meat system. Incubated at 37C for 72 h in BHI, gentistic, benzoic and vanillic acids inhibited L.m., E.c and S.T. at 5,000 µg/mL by 2.8 to 3.0 log CFU/mL, 2.8 to ...
DOI:
10.1111/j.1745-4565.2011.00322.x

93. Environmental Conditions in Beef Deep-Bedded Mono-Slope Facilities: A Descriptive Study

Author:
Spiehs, M.J.; Woodbury, B.L.; Doran, B.E.; Eigenberg, R.A.; Kohl, K.D.; Varel, V.H.; Berry, E.D.; Wells, J.E.
Source:
Transactions of the ASABE 2011 v.54 no.2 pp. 663-673
ISSN:
2151-0032
Subject:
Escherichia coli O157; air; air temperature; ammonia; barns; beef; cattle feeding; cleaning; cold; descriptive studies; environmental factors; excretion; gas emissions; humidity; nutrient content; odors; pH; pathogens; volatile organic compounds; water content; Great Plains region
Abstract:
... In the Northern Great Plains, interest in feeding cattle in enclosed beef deep-bedded mono-slope facilities (BDMF) has increased. Characterization of environmental factors impacting odor and gas emissions, nutrient excretion, and pathogens is needed to develop recommendations for management of BDMF. The objectives of this study were to determine spatial variability of ammonia (NH 3 ) in the air an ...

94. An Escherichia coli O157-Specific Engineered Pyocin Prevents and Ameliorates Infection by E. coli O157:H7 in an Animal Model of Diarrheal Disease

Author:
Jennifer M. Ritchie; Jennifer L. Greenwich; Brigid M. Davis; Roderick T. Bronson; Dana Gebhart; Steven R. Williams; David Martin; Dean Scholl; Matthew K. Waldor
Source:
Antimicrobial agents and chemotherapy 2011 v.55 no.12 pp. 5469-5474
ISSN:
0066-4804
Subject:
Escherichia coli O157; animal models; antibiotics; diarrhea; inflammation; intestines; pathogens; rabbits; therapeutics
Abstract:
... AvR2-V10.3 is an engineered R-type pyocin that specifically kills Escherichia coli O157, an enteric pathogen that is a major cause of food-borne diarrheal disease. New therapeutics to counteract E. coli O157 are needed, as currently available antibiotics can exacerbate the consequences of infection. We show here that orogastric administration of AvR2-V10.3 can prevent or ameliorate E. coli O157:H7 ...
DOI:
10.1128/AAC.05031-11
PubMed:
21947394
PubMed Central:
PMC3232761

95. Escherichia coli O157:H7 Genetic Diversity in Bovine Fecal Samples

Author:
M. E. Jacob; K. M. Almes; X. Shi; J. M. Sargeant; T. G. Nagaraja
Source:
Journal of food protection 2011 v.74 no.7 pp. 1186-1188
ISSN:
0362-028X
Subject:
Escherichia coli O157; beef cattle; feces; food contamination; foodborne illness; foods; genes; genetic variation; humans; immunomagnetic separation; polymerase chain reaction; pulsed-field gel electrophoresis; selective media; virulence
Abstract:
... Escherichia coli O157:H7 causes foodborne illness in humans; cattle are considered a primary reservoir for the organism, and transmission is often through contaminated food products or water. The objective of this study was to determine the genetic diversity of E. coli O157:H7 within a single individual bovine fecal sample based on pulsed-field gel electrophoresis (PFGE) typing. Fecal samples (n = ...
DOI:
10.4315/0362-028X.JFP-11-022

96. Escherichia coli O157:H7 Shedding in Vaccinated Beef Calves Born to Cows Vaccinated Prepartum with Escherichia coli O157:H7 SRP Vaccine

Author:
B. W. Wileman; D. U. Thomson; K. C. Olson; J. R. Jaeger; L. A. Pacheco; J. Bolte; D. T. Burkhardt; D. A. Emery; D. Straub
Source:
Journal of food protection 2011 v.74 no.10 pp. 1599-1604
ISSN:
0362-028X
Subject:
Escherichia coli O157; antibodies; beef cattle; calves; cows; equipment; feedlots; liver abscess; morbidity; mortality; pathogens; slaughter; vaccination; vaccines
Abstract:
... Extensive research, intervention equipment, money, and media coverage have been directed at controlling Escherichia coli O157:H7 in beef cattle. However, much of the focus has been on controlling this pathogen postcolonization. This study was conducted to examine the performance, health, and shedding characteristics of beef calves that were vaccinated with an E. coli O157:H7 SRP bacterial extract. ...
DOI:
10.4315/0362-028X.JFP-11-034

97. Escherichia coli O157:H7 in beef cattle: on farm contamination and pre-slaughter control methods

Author:
J. M. Soon; S. A. Chadd; R. N. Baines
Source:
Animal health research reviews 2011 v.12 no.2 pp. 197-211
ISSN:
1475-2654
Subject:
Escherichia coli O157; bacteria; beef; beef cattle; cattle production; control methods; cross contamination; farms; food handling; food safety; foodborne illness; risk; risk reduction; slaughter; slaughterhouses
Abstract:
... This paper addresses food safety in beef cattle production, with particular emphasis on factors that affect the prevalence of Escherichia coli O157:H7 in beef cattle and on control methods that have been investigated. Product recalls and foodborne diseases due to this organism continue to occur even though control measures have been under investigation for over 20 years. Most meatborne outbreaks a ...
DOI:
10.1017/S1466252311000132

98. Escherichia coli O157:H7 populations in ruminants can be reduced by orange peel product feeding

Author:
Todd R. Callaway; Jeffery A. Carroll; John D. Arthington; Tom S. Edrington; Michelle L. Rossman; Mandy A. Carr; Nathan A. Krueger; Steven C. Ricke; Phil Crandall; David J. Nisbet
Source:
Journal of food protection 2011 v.74 no. pp. 1917-1921
ISSN:
1944-9097
Subject:
Escherichia coli O157; antimicrobial properties; byproducts; cecum; corn; diet; disease control; experimental design; intestinal microorganisms; orange peels; orange pulp; pathogens; pellets; rectum; rumen; sheep; sheep feeding
Abstract:
... Foodborne pathogenic bacteria such as Escherichia coli O157:H7 are threats to the safety of beef. Citrus peel and dried orange pulp are by-products from citrus juice production that have natural antimicrobial effects and are often incorporated into least-cost ration formulations for beef and dairy cattle. This study was designed to determine if orange peel and pulp affected E. coli O157:H7 populat ...
Handle:
10113/57239
DOI:
10.4315/0362-028X.JFP-11-234

99. European Starlings (Sturnus vulgaris) challenged with Escherichia coli O157 can carry and transmit the human pathogen to cattle

Author:
M.D. Kauffman; J. LeJeune
Source:
Letters in applied microbiology 2011 v.53 no.6 pp. 596-601
ISSN:
0266-8254
Subject:
Escherichia coli O157; Sturnus vulgaris; biosecurity; birds; calves; farms; feces; health hazards; humans; invasive species; pathogens; pollution; public health; United States
Abstract:
... Aims: European starlings (Sturnus vulgaris) are an invasive species in the United States and are considered a nuisance pest to agriculture. The goal of this study was to determine the potential for these birds to be reservoirs and/or vectors for the human pathogen Escherichia coli O157:H7. Materials and Results: Under biosecurity confinement, starlings were challenged with various doses of E. coli ...
DOI:
10.1111/j.1472-765X.2011.03163.x
PubMed:
21985308

100. Evaluation of Escherichia coli O157:H7 Translocation and Decontamination for Beef Vacuum-Packaged Subprimals Destined for Nonintact Use

Author:
Jacob L. Lemmons; Lisa M. Lucia; Margaret D. Hardin; Jeffrey W. Savell; Kerri B. Harris
Source:
Journal of food protection 2011 v.74 no.7 pp. 1048-1053
ISSN:
0362-028X
Subject:
Escherichia coli O157; beef; decontamination; pathogens; vacuum packaging; washing
Abstract:
... The translocation of Escherichia coli O157:H7 as well as the impact of water washing and partial or complete surface trimming as possible pathogen reduction strategies were evaluated for vacuum-packaged beef subprimals destined for nonintact use. Cap-on and cap-off beef top sirloin butts were inoculated with two levels of E. coli O157:H7: a high-inoculum level of approximately 10(4) CFU/cm2 and a ...
DOI:
10.4315/0362-028X.JFP-10-535