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You searched for: Publication Year 2010 Remove constraint Publication Year: 2010 Subject active sites Remove constraint Subject: active sites Subject amino acids Remove constraint Subject: amino acids Text Availability Citation in PubAg Remove constraint Text Availability: Citation in PubAg
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1. Analysis and Comparison of Biochemical and Molecular Structural Characteristics and Phylogenetic Trend of Two h-TypeThioredoxin Isoforms from Grape (Vitis vinifera L. cv. Askari)

Author:
reza Heydari; raheem Haddad; GHasssemali Garoosi
Source:
Biyutiknuluzhī-i kishāvarzī 2010 v.2 no.1 pp. 25-43
ISSN:
2228-6500
Subject:
Vitis vinifera; active sites; amino acid sequences; amino acids; genes; grapes; heat stability; isoelectric point; molecular weight; phylogeny; plasmid vectors; polypeptides; research projects; reverse transcription; sequence analysis; thioredoxins
Abstract:
... Thioredoxins (Trxs) are small heat-stable proteins that participate in dithiol-disulfide exchange reactions. In contrast to other organisms, plants contain six different Trx types: f, m, x, y, o and h. The h-type Trx consists of multiple forms that involved in different processes such as cellular protection against oxidative, biotic an ...
DOI:
10.22103/jab.2011.359

2. Antifungal chitinases from Bacillus pumilus SG2: preliminary report

Author:
Ghasemi, Seyedhadi; Ahmadian, Gholamreza; Jelodar, Nadali Babaeian; Rahimian, Heshmatollah; Ghandili, Soheila; Dehestani, Ali; Shariati, Parvin
Source:
World journal of microbiology & biotechnology 2010 v.26 no.8 pp. 1437-1443
ISSN:
0959-3993
Subject:
Bacillus pumilus; Bipolaris; Nigrospora; Phytophthora capsici; Phytophthora citricola; Thanatephorus cucumeris; Verticillium; active sites; amino acids; ammonium sulfate; antifungal properties; bacteria; cell walls; chitin; chitinase; fungi; marshes; oligosaccharides; pH; saline soils; salt tolerance; temperature; Iran
Abstract:
... A halotolerant bacterium capable of chitinase production was previously isolated from saline soils of Gavkhooni marsh, Iran. The strain was identified as Bacillus pumilus strain SG2. This strain secretes two chitinases into the medium in response to the presence of chitin in the medium. The two chitinases, ChiS and ChiL, were active on both polymeric as well as oligosaccharide substrates. In this ...
DOI:
10.1007/s11274-010-0318-6

3. Arabidopsis CYP72C1 is an atypical cytochrome P450 that inactivates brassinosteroids

Author:
Leeann E. Thornton; Sanjeewa G. Rupasinghe; Hao Peng; Mary A. Schuler; Michael M. Neff
Source:
Plant molecular biology 2010 v.74 no.1-2 pp. 167-181
ISSN:
0167-4412
Subject:
Arabidopsis thaliana; active sites; amino acids; binding sites; brassinolide; cytochrome P-450; enzymes; genetic techniques and protocols; growth and development; hormones; metabolism; plant development; seedling growth; signal transduction
Abstract:
... Cytochrome P450 monooxygenases (P450s) are a diverse family of proteins that have specialized roles in secondary metabolism and in normal cell development. Two P450s in particular, CYP734A1 and CYP72C1, have been identified as brassinosteroid-inactivating enzymes important for steroid-mediated signal transduction in Arabidopsis thaliana. Genetic analyses have demonstrated that these P450s modulate ...
DOI:
10.1007/s11103-010-9663-y
PubMed:
20669042

4. Beyond antibiotic resistance: integrating conjugative elements of the SXT/R391 family that encode novel diguanylate cyclases participate to c-di-GMP signalling in Vibrio cholerae

Author:
Bordeleau, Eric; Brouillette, Eric; Robichaud, Nathaniel; Burrus, Vincent
Source:
Environmental microbiology 2010 v.12 no.2 pp. 510-523
ISSN:
1462-2912
Subject:
Vibrio cholerae; active sites; amino acids; antibiotic resistance; aquatic environment; bacterial motility; biofilm; enzyme activity; enzymes; exopolysaccharides; gene expression; genes; guanosine triphosphate; hosts; phenotype; phosphorylation; second messengers; virulence; Asia; Central America
Abstract:
... In Vibrio cholerae, the second messenger bis-(3′-5′)-cyclic dimeric guanosine monophosphate (c-di-GMP) increases exopolysaccharides production and biofilm formation and decreases virulence and motility. As such, c-di-GMP is considered an important player in the transition from the host to persistence in the environment. c-di-GMP level is regulated through a complex network of more than 60 chromoso ...
DOI:
10.1111/j.1462-2920.2009.02094.x
PubMed:
19888998

5. C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli

Author:
Ribitsch, D.; Karl, W.; Birner-Gruenberger, R.; Gruber, K.; Eiteljoerg, I.; Remler, P.; Wieland, S.; Siegert, P.; Maurer, K.H.; Schwab, H.
Source:
Journal of biotechnology 2010 v.150 no.3 pp. 408-416
ISSN:
0168-1656
Subject:
Escherichia coli; Stenotrophomonas maltophilia; Xanthomonas campestris; active sites; amino acids; biotechnology; hosts; insulin; metagenomics; models; pH; screening; serine proteinases; washing
Abstract:
... Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli a ...
DOI:
10.1016/j.jbiotec.2010.09.947

6. Characterization of the family GH54 α-l-arabinofuranosidases in Penicillium funiculosum, including a novel protein bearing a cellulose-binding domain

Author:
Guais, Olivier; Tourrasse, Olivier; Dourdoigne, Marion; Parrou, Jean Luc; Francois, Jean Marie
Source:
Applied microbiology and biotechnology 2010 v.87 no.3 pp. 1007-1021
ISSN:
0175-7598
Subject:
Talaromyces funiculosus; active sites; alpha-N-arabinofuranosidase; amino acids; arabinose; arabinoxylan; cellulose; fermentation; fungi; genes; pH; proteins; sequence analysis; soil; temperature; thermal stability
Abstract:
... The soil deuteromycete Penicillium funiculosum is characterized by its remarkable capacity to produce a wide variety of cellulolytic and hemicellulolytic enzymes. In the course of the genome sequencing of this industrial fungus, four different genes encoding glycosyl hydrolase family 54 (GH54)22 α-l-arabinofuranosidases were identified. Three of them termed PfabfB1, PfabfB3, and PfabfB4 were highl ...
DOI:
10.1007/s00253-010-2532-4

7. Cloning and characterization of a ribitol dehydrogenase from Zymomonas mobilis

Author:
Moon, Hee-Jung; Tiwari, Manish; Jeya, Marimuthu; Lee, Jung-Kul
Source:
Applied microbiology and biotechnology 2010 v.87 no.1 pp. 205-214
ISSN:
0175-7598
Subject:
DNA; Escherichia coli; NAD (coenzyme); NADP (coenzyme); Zymomonas mobilis; active sites; affinity chromatography; amino acids; gel chromatography; gels; gene overexpression; genes; glutathione transferase; models; molecular weight; open reading frames; pH; polypeptides; sequence analysis; sodium dodecyl sulfate; temperature
Abstract:
... Ribitol dehydrogenase (RDH) catalyzes the conversion of ribitol to d-ribulose. A novel RDH gene was cloned from Zymomonas mobilis subsp. mobilis ZM4 and overexpressed in Escherichia coli BL21(DE3). DNA sequence analysis revealed an open reading frame of 795 bp, capable of encoding a polypeptide of 266 amino acid residues with a calculated molecular mass of 28,426 Da. The gene was overexpressed in ...
DOI:
10.1007/s00253-010-2444-3
PubMed:
20127234

8. Collagenolytic subtilisin-like protease from the deep-sea bacterium Alkalimonas collagenimarina AC40T

Author:
Kurata, Atsushi; Uchimura, Kohsuke; Kobayashi, Tohru; Horikoshi, Koki
Source:
Applied microbiology and biotechnology 2010 v.86 no.2 pp. 589-598
ISSN:
0175-7598
Subject:
EDTA (chelating agent); Escherichia coli; active sites; amino acids; bacteria; casein; collagen; gelatin; genes; open reading frames; proteinases
Abstract:
... A new alkaline protease (AcpII) was purified from a culture of the deep-sea bacterium Alkalimonas collagenimarina AC40T. AcpII degraded collagen three times faster than it degraded casein. The optimal pH was 8.5-9, and the optimal temperature was 45°C for the degradation of collagen. AcpII was completely inhibited by phenylmethylsulfonyl fluoride and partially by EDTA. Cloning and sequencing the g ...
DOI:
10.1007/s00253-009-2324-x
PubMed:
19888572

9. Deimination is regulated at multiple levels including auto-deimination of peptidylarginine deiminases

Author:
Méchin, Marie-Claire; Coudane, Fanny; Adoue, Véronique; Arnaud, Jacques; Duplan, Hélène; Charveron, Marie; Schmitt, Anne-Marie; Takahara, Hidenari; Serre, Guy; Simon, Michel
Source:
Cellular and molecular life sciences 2010 v.67 no.9 pp. 1491-1503
ISSN:
1420-682X
Subject:
active sites; amino acids; gene expression regulation; genes; keratinocytes; messenger RNA; proteins; translation (genetics); vitamin D
Abstract:
... Peptidylarginine deiminases (PADs) catalyze deimination, converting arginyl to citrullyl residues. Only three PAD isotypes are detected in the epidermis where they play a crucial role, targeting filaggrin, a key actor for the tissue hydration and barrier functions. Their expression and activation depends on the keratinocyte differentiation state. To investigate this regulation, we used primary ker ...
DOI:
10.1007/s00018-010-0262-5

10. Genetic and biochemical characterization of poly 3-hydroxybutyrate depolymerase from Diaphorobacter sp. PCA039

Author:
Zhang, Tao; Chaudhry, Muhammad Tausif; Liu, Zhi-Pei
Source:
World journal of microbiology & biotechnology 2010 v.26 no.10 pp. 1803-1811
ISSN:
0959-3993
Subject:
Escherichia coli; active sites; amino acid sequences; amino acids; carbon; fibronectins; gene overexpression; genes; genomic libraries; molecular weight; nucleotide sequences; pH; polyhydroxybutyrate; screening; sequence analysis; temperature; triacylglycerol lipase
Abstract:
... Diaphorobacter sp. PCA039 secreted a polyhydroxybutyrate (PHB) depolymerase into the culture supernatant when it was cultivated on poly 3-PHB or poly 3-hydroxybutyrate-co-3-hydroxyvalerate [PHB(V)] as the sole carbon source. A PHB(V) depolymerase-encoding gene (phaZd) was cloned by construction of a Fosmid genomic library and degenerate primer screening, and overexpressed in Escherichia coli BL21 ...
DOI:
10.1007/s11274-010-0361-3

11. Hydrogen production in nitrogenase mutants in Anabaena variabilis

Author:
Weyman, Philip D.; Pratte, Brenda; Thiel, Teresa
Source:
FEMS microbiology letters 2010 v.304 no.1 pp. 55-61
ISSN:
0378-1097
Subject:
Anabaena variabilis; acetylene reduction; active sites; amino acids; ammonia; anaerobic conditions; argon (noble gases); fructose; gases; hydrogen; hydrogen production; mutants; nitrogen; nitrogenase; stable isotopes; vegetative cells
Abstract:
... Nitrogenase produces hydrogen as a normal byproduct of the reduction of dinitrogen to ammonia. The Nif2 nitrogenase in Anabaena variabilis is an alternative Mo-nitrogenase and is expressed in vegetative cells grown with fructose under strictly anaerobic conditions. We report here that the V75I substitution in the α-subunit of Nif2 showed greatly impaired acetylene reduction and reduced levels of ¹ ...
DOI:
10.1111/j.1574-6968.2009.01883.x
PubMed:
20070369

12. Identification and characterization of a null-activity mutant containing a cryptic pre-mRNA splice site for cytosolic fructose-1,6-bisphosphatase in Flaveria linearis

Author:
Slater, S. M. H.; Micallef, M. C.; Zhang, J.; Micallef, B. J.
Source:
Plant molecular biology 2010 v.74 no.6 pp. 519-536
ISSN:
0167-4412
Subject:
Flaveria; active sites; alternative splicing; amino acids; enzyme activity; epistasis; fructose-bisphosphatase; messenger RNA; mutants; phenotype; phosphofructokinases; photosynthesis; point mutation; starch; structural genes; sucrose
Abstract:
... Cytosolic fructose-1,6-bisphosphatase (cytFBPase) (E.C. 3.1.3.11) catalyzes the first irreversible reaction of daytime sucrose synthesis. A Flaveria linearis (F. linearis) mutant (line 84-9) previously shown to have ~10% wildtype cytFBPase activity contains no cytFBPase activity based on enzymatic and immunoprecipitation analysis. Genetic segregation and Southern analysis of an F2 population shows ...
DOI:
10.1007/s11103-010-9690-8
PubMed:
20882321

13. Molecular cloning, characterization and function analysis of the gene encoding HMG-CoA reductase from Euphorbia Pekinensis Rupr

Author:
Cao, Xiaoying; Zong, Zhimin; Ju, Xiuyun; Sun, Yong; Dai, Chuanchao; Liu, Qun; Jiang, Jihong
Source:
Molecular biology reports 2010 v.37 no.3 pp. 1559-1567
ISSN:
0301-4851
Subject:
Southern blotting; biosynthesis; complementary DNA; open reading frames; stems; isoprenoids; bioinformatics; rapid amplification of cDNA ends; amino acids; genes; active sites; color; hydroxymethylglutaryl-CoA reductases; molecular cloning; roots; Euphorbia; leaves; Escherichia coli
Abstract:
... A new full-length cDNA encoding 3-hydroxy-3-methylglutoryl-Coenzyme A reductase (HMGR; EC1.1.1.34), which catalyzes the first committed step of isoprenoids biosynthesis in MVA pathway, was isolated from young leaves of Euphorbia Pekinensis Rupr. by rapid amplification of cDNA ends (RACE) for the first time. The full-length cDNA of HMGR (designated as EpHMGR, GenBank Accession NO. EF062569) was 2,2 ...
DOI:
10.1007/s11033-009-9558-7
PubMed:
19437136

14. Purification, characterization and molecular cloning of a novel endo-β-N-acetylglucosaminidase from the basidiomycete, Flammulina velutipes

Author:
Hamaguchi, Tasuku; Ito, Tsukasa; Inoue, Yukako; Limpaseni, Tipaporn; Pongsawasdi, Piamsook; Ito, Kazuo
Source:
Glycobiology 2010 v.20 no.4 pp. 420-432
ISSN:
0959-6658
Subject:
Escherichia coli; Flammulina velutipes; active sites; amino acids; chitinase; complementary DNA; enzyme activity; fruiting bodies; fungi; glycoproteins; glycosides; introns; mannose; metabolism; models; molecular cloning; oligosaccharides; open reading frames; substrate specificity
Abstract:
... Endo-β-N-acetylglucosaminidases are thought to be key enzymes in the catabolism of asparagine-linked oligosaccharides. However, little is known about the enzymes of this type in basidiomycetes. We investigated endo-β-N-acetylglucosaminidases in basidiomycetes using fluorescence-labeled glycoasparagines as substrates. Flammulina velutipes showed high activity and its enzyme was named endo-β-N-acety ...
DOI:
10.1093/glycob/cwp188

15. The RNA dreamtime: modern cells feature proteins that might have supported a prebiotic polypeptide world but nothing indicates that RNA world ever was

Author:
Kurland, Charles G.
Source:
BioEssays 2010 v.32 no.10 pp. 866-871
ISSN:
0265-9247
Subject:
RNA; active sites; amino acids; polypeptides; prebiotics; proteolysis; ribonucleoproteins
Abstract:
... Modern cells present no signs of a putative prebiotic RNA world. However, RNA coding is not a sine qua non for the accumulation of catalytic polypeptides. Thus, cellular proteins spontaneously fold into active structures that are resistant to proteolysis. The law of mass action suggests that binding domains are stabilized by specific interactions with their substrates. Random polypeptide synthesis ...
DOI:
10.1002/bies.201000058

16. Superstoichiometric binding of L-Phe to phenylalanine hydroxylase from Caenorhabditis elegans: evolutionary implications

Author:
Flydal, Marte I.; Mohn, Tonje C.; Pey, Angel L.; Siltberg-Liberles, Jessica; Teigen, Knut; Martinez, Aurora
Source:
Amino acids 2010 v.39 no.5 pp. 1463-1475
ISSN:
0939-4451
Subject:
Caenorhabditis elegans; active sites; amino acids; binding proteins; binding sites; calorimetry; catalytic activity; humans; hydroxylation; mutants; phenylalanine 4-monooxygenase; phenylketonuria; polycyclic aromatic hydrocarbons; protein subunits; titration
Abstract:
... Phenylalanine hydroxylase (PAH) catalyzes the hydroxylation of L-Phe to L-Tyr. Dysfunctional PAH results in phenylketonuria and mammalian PAH is therefore highly regulated and displays positive cooperativity for L-Phe (Hill coefficient (h) = 2). L-Phe does not bind to the regulatory ACT domain in full-length tetrameric human PAH and cooperativity is elicited by homotropic binding to the catalytic ...
DOI:
10.1007/s00726-010-0611-6

17. Two novel thermostable chitinase genes from thermophilic fungi: Cloning, expression and characterization

Author:
Li, An-Na; Yu, Kai; Liu, Hai-Quan; Zhang, Jie; Li, Hua; Li, Duo-Chuan
Source:
Bioresource technology 2010 v.101 no.14 pp. 5546-5551
ISSN:
0960-8524
Subject:
temperature; Komagataella pastoris; clones; amino acids; thermophilic fungi; genes; active sites; hydrolysis; pH; Chaetomium; molecular weight; catalytic activity; chitin; chitinase
Abstract:
... Two chitinase genes, Tachit1 from Thermoascus aurantiacus var. levisporus and Ctchit1 from Chaetomium thermophilum were isolated. Tachit1 and Ctchit1 encode putative single-domain proteins (TaCHIT1 and CtCHIT1) of 399 and 402 amino acid residues, respectively. The catalytic domains of TaCHIT1 and CtCHIT1 are similar to those of other fungal chitinases in family 18 of glycosyl hydrolases. TaCHIT1 a ...
DOI:
10.1016/j.biortech.2010.02.058
PubMed:
20223653

18. A new β-glucosidase gene from the zygomycete fungus Rhizomucor miehei

Author:
Takó, Miklós; Tóth, Adél; Nagy, László G.; Krisch, Judit; Vágvölgyi, Csaba; Papp, Tamás
Source:
Antonie van Leeuwenhoek 2010 v.97 no.1 pp. 1-10
ISSN:
0003-6072
Subject:
Mucor; proteins; Rhizomucor miehei; plasmids; amino acids; active sites; introns; sequence homology; beta-glucosidase; fungi
Abstract:
... In this study, a β-glucosidase coding gene (bgl) of the zygomycete fungus Rhizomucor miehei has been cloned and characterized. The gene comprises a total of 2,826 bp including the coding sequence of a 717 amino acids length putative protein and 10 introns dispersed in the whole coding region. The putative N-and C-terminal catalytic domains (aa 68 to aa 274 and aa 358-601, respectively) were identi ...
DOI:
10.1007/s10482-009-9382-z

19. The starch-binding capacity of the noncatalytic SBD2 region and the interaction between the N- and C-terminal domains are involved in the modulation of the activity of starch synthase III from Arabidopsis thaliana

Author:
Wayllace, Nahuel Z.; Valdez, Hugo A.; Ugalde, Rodolfo A.; Busi, Maria V.; Gomez-Casati, Diego F.
Source:
FEBS journal 2010 v.277 no.2 pp. 428-440
ISSN:
1742-464X
Subject:
Arabidopsis thaliana; Western blotting; active sites; amino acids; mutants; proteins; starch; starch synthase
Abstract:
... Starch synthase III from Arabidopsis thaliana contains an N-terminal region, including three in-tandem starch-binding domains, followed by a C-terminal catalytic domain. We have reported previously that starch-binding domains may be involved in the regulation of starch synthase III function. In this work, we analyzed the existence of protein interactions between both domains using pull-down assays ...
DOI:
10.1111/j.1742-4658.2009.07495.x
PubMed:
19968859