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1. 4-Deoxy-substrates for β-N-acetylhexosaminidases: How to make use of their loose specificity

Author:
Slámová, Kristýna; Gažák, Radek; Bojarová, Pavla; Kulik, Natallia; Ettrich, Rudiger; Pelantová, Helena; Sedmera, Petr; Křen, Vladimír
Source:
Glycobiology 2010 v.20 no.8 pp. 1002-1009
ISSN:
0959-6658
Subject:
Aspergillus oryzae; Talaromyces flavus; active sites; beta-N-acetylhexosaminidase; computer simulation; fungi; glycosylation; humans; hydrolysis; models; moieties; screening
Abstract:
... β-N-Acetylhexosaminidases feature so-called wobbling specificity, which means that they cleave substrates both in gluco- and galacto- configurations, with the activity ratio depending on the enzyme source. Here we present the new finding that fungal β-N-acetylhexosaminidases are able to hydrolyze and transfer 4-deoxy-N-acetylhexosaminides with high yields. This clearly demonstrates that the 4-hydr ...
DOI:
10.1093/glycob/cwq058

2. Active and regulatory sites of cytosolic 5′‐nucleotidase

Author:
Pesi, Rossana; Allegrini, Simone; Careddu, Maria Giovanna; Filoni, Daniela Nicole; Camici, Marcella; Tozzi, Maria Grazia
Source:
FEBS journal 2010 v.277 no.23 pp. 4863-4872
ISSN:
1742-464X
Subject:
active sites; adenosine diphosphate; adenosine triphosphate; aspartic acid; crystal structure; enzyme activation; hydrolysis; light scattering; models; nucleosides; phosphates; point mutation
Abstract:
... Cytosolic 5′‐nucleotidase (cN‐II), which acts preferentially on 6‐hydroxypurine nucleotides, is essential for the survival of several cell types. cN‐II catalyses both the hydrolysis of nucleotides and transfer of their phosphate moiety to a nucleoside acceptor through formation of a covalent phospho‐intermediate. Both activities are regulated by a number of phosphorylated compounds, such as diaden ...
DOI:
10.1111/j.1742-4658.2010.07891.x

3. Adenylyl cyclase regulates heavy metal sensitivity, bikaverin production and plant tissue colonization in Fusarium proliferatum

Author:
Kohut, Gábor; Oláh, Brigitta; Ádám, Attila L.; García-Martínez, Jorge; Hornok, László
Source:
Journal of basic microbiology 2010 v.50 no.1 pp. 59-71
ISSN:
0233-111X
Subject:
DNA primers; Fusarium proliferatum; Neurospora crassa; active sites; adenylate cyclase; cadmium; conidia; conidiation; conserved sequences; copper; corn; crossing; female fertility; fruits; fungi; gene targeting; genes; genomic libraries; germination; heavy metals; male fertility; metabolites; mutants; nitrogen; plant tissues; polymerase chain reaction; proteins; screening; seedlings; signal transduction; stress response; tomatoes; vegetative growth
Abstract:
... A homologue of the adenylyl cyclase (AC) gene of Neurospora crassa, named Fpacy1 was cloned from the genomic library of Fusarium proliferatum ITEM 2287 by screening the library with a DNA fragment amplified by using PCR primers designed from conserved sequences of the catalytic domain of AC genes from other fungi. The deduced FPACY1 protein had 53-77% identity with the AC proteins of other fungi. ...
DOI:
10.1002/jobm.200900113

4. Alkali-Stabilized Pt-OHx Species Catalyze Low-Temperature Water-Gas Shift Reactions

Author:
Zhai, Yanping; Pierre, Danny; Si, Rui; Deng, Weiling; Ferrin, Peter; Nilekar, Anand U.; Peng, Guowen; Herron, Jeffrey A.; Bell, David C.; Saltsburg, Howard; Mavrikakis, Manos; Flytzani-Stephanopoulos, Maria
Source:
Science 2010 v.329 no.5999 pp. 1633-1636
ISSN:
0036-8075
Subject:
active sites; aluminum oxide; carbon dioxide; catalysts; hydrogen; ions; methodology; platinum; potassium; silica; sodium; temperature
Abstract:
... We report that alkali ions (sodium or potassium) added in small amounts activate platinum adsorbed on alumina or silica for the low-temperature water-gas shift (WGS) reaction (H₂O + CO [rightward arrow] H₂ + CO₂) used for producing H₂. The alkali ion-associated surface OH groups are activated by CO at low temperatures (approximately 100°C) in the presence of atomically dispersed platinum. Both exp ...
DOI:
10.1126/science.1192449

5. Allosteric Supramolecular Triple-Layer Catalysts

Author:
Yoon, Hyo Jae; Kuwabara, Junpei; Kim, Jun-Hyun; Mirkin, Chad A.
Source:
Science 2010 v.330 no.6000 pp. 66-69
ISSN:
0036-8075
Subject:
active sites; anions; catalysts; catalytic activity; chlorides; molecular weight; polymerization; polymers
Abstract:
... Allosteric regulation of organometallic catalysts could allow for greater control over reactions. We report an allosteric supramolecular structure in which a monometallic catalytic site has been buried in the middle layer of a triple-layer complex. Small molecules and elemental anions can open and close this complex and reversibly expose and conceal the catalytic center. The ring-opening polymeriz ...
DOI:
10.1126/science.1193928

6. Analysing and understanding the active site by IR spectroscopy

Author:
Alexandre Vimont; Frédéric Thibault-Starzyk; Marco Daturi
Source:
Chemical Society reviews 2010 v.39 no.12 pp. 4928-4950
ISSN:
1460-4744
Subject:
active sites; adsorption; catalytic activity; infrared spectroscopy; physicochemical properties; quantitative analysis
Abstract:
... IR spectroscopy is a technique particularly adapted for understanding the mechanism of catalytic reactions, being able to probe the surface mechanisms at the molecular level. In this critical review the main advances in the field are presented, both under the aspects of the in situ and operando approaches. A broad view of the most authoritative literature of the domain is given, based largely on t ...
DOI:
10.1039/b919543m

7. Analysis and Comparison of Biochemical and Molecular Structural Characteristics and Phylogenetic Trend of Two h-TypeThioredoxin Isoforms from Grape (Vitis vinifera L. cv. Askari)

Author:
reza Heydari; raheem Haddad; GHasssemali Garoosi
Source:
Biyutiknuluzhī-i kishāvarzī 2010 v.2 no.1 pp. 25-43
ISSN:
2228-6500
Subject:
Vitis vinifera; active sites; amino acid sequences; amino acids; genes; grapes; heat stability; isoelectric point; molecular weight; phylogeny; plasmid vectors; polypeptides; research projects; reverse transcription; sequence analysis; thioredoxins
Abstract:
... Thioredoxins (Trxs) are small heat-stable proteins that participate in dithiol-disulfide exchange reactions. In contrast to other organisms, plants contain six different Trx types: f, m, x, y, o and h. The h-type Trx consists of multiple forms that involved in different processes such as cellular protection against oxidative, biotic an ...
DOI:
10.22103/jab.2011.359

8. Antifungal chitinases from Bacillus pumilus SG2: preliminary report

Author:
Ghasemi, Seyedhadi; Ahmadian, Gholamreza; Jelodar, Nadali Babaeian; Rahimian, Heshmatollah; Ghandili, Soheila; Dehestani, Ali; Shariati, Parvin
Source:
World journal of microbiology & biotechnology 2010 v.26 no.8 pp. 1437-1443
ISSN:
0959-3993
Subject:
Bacillus pumilus; Bipolaris; Nigrospora; Phytophthora capsici; Phytophthora citricola; Thanatephorus cucumeris; Verticillium; active sites; amino acids; ammonium sulfate; antifungal properties; bacteria; cell walls; chitin; chitinase; fungi; marshes; oligosaccharides; pH; saline soils; salt tolerance; temperature; Iran
Abstract:
... A halotolerant bacterium capable of chitinase production was previously isolated from saline soils of Gavkhooni marsh, Iran. The strain was identified as Bacillus pumilus strain SG2. This strain secretes two chitinases into the medium in response to the presence of chitin in the medium. The two chitinases, ChiS and ChiL, were active on both polymeric as well as oligosaccharide substrates. In this ...
DOI:
10.1007/s11274-010-0318-6

9. Arabidopsis CYP72C1 is an atypical cytochrome P450 that inactivates brassinosteroids

Author:
Leeann E. Thornton; Sanjeewa G. Rupasinghe; Hao Peng; Mary A. Schuler; Michael M. Neff
Source:
Plant molecular biology 2010 v.74 no.1-2 pp. 167-181
ISSN:
0167-4412
Subject:
Arabidopsis thaliana; active sites; amino acids; binding sites; brassinolide; cytochrome P-450; enzymes; genetic techniques and protocols; growth and development; hormones; metabolism; plant development; seedling growth; signal transduction
Abstract:
... Cytochrome P450 monooxygenases (P450s) are a diverse family of proteins that have specialized roles in secondary metabolism and in normal cell development. Two P450s in particular, CYP734A1 and CYP72C1, have been identified as brassinosteroid-inactivating enzymes important for steroid-mediated signal transduction in Arabidopsis thaliana. Genetic analyses have demonstrated that these P450s modulate ...
DOI:
10.1007/s11103-010-9663-y
PubMed:
20669042

10. Aspartic Cathepsin D Endopeptidase Contributes to Extracellular Digestion in Clawed Lobsters Homarus americanus and Homarus gammarus

Author:
Rojo, Liliana; Muhlia-Almazan, Adriana; Saborowski, Reinhard; García-Carreño, Fernando
Source:
Marine biotechnology 2010 v.12 no.6 pp. 696-707
ISSN:
1436-2228
Subject:
Homarus americanus; Homarus gammarus; active sites; amino acid sequences; cathepsin D; complementary DNA; cysteine proteinases; digestion; digestive enzymes; gastric juice; glycosylation; gonads; insects; lobsters; messenger RNA; midgut; molecular weight; muscles; quantitative polymerase chain reaction; starvation
Abstract:
... Acid digestive proteinases were studied in the gastric fluids of two species of clawed lobster (Homarus americanus and Homarus gammarus). An active protein was identified in both species as aspartic proteinase by specific inhibition with pepstatin A. It was confirmed as cathepsin D by mass mapping, N-terminal, and full-length cDNA sequencing. Both lobster species transcribed two cathepsin D mRNAs: ...
DOI:
10.1007/s10126-010-9257-3

11. Asymmetric synthesis of (S)-3-chloro-1-phenyl-1-propanol using Saccharomyces cerevisiae reductase with high enantioselectivity

Author:
Choi, Yun Hee; Choi, Hye Jeong; Kim, Dooil; Uhm, Ki-Nam; Kim, Hyung-Kwoun
Source:
Applied microbiology and biotechnology 2010 v.87 no.1 pp. 185-193
ISSN:
0175-7598
Subject:
Escherichia coli; NADP (coenzyme); Saccharomyces cerevisiae; Sporobolomyces; active sites; antidepressants; chromatography; enantiomers; energy; genetic databases; glucose; loci; models; pH; sodium hydroxide; temperature; yeasts
Abstract:
... 3-Chloro-1-phenyl-1-propanol is used as a chiral intermediate in the synthesis of antidepressant drugs. Various microbial reductases were expressed in Escherichia coli, and their activities toward 3-chloro-1-phenyl-1-propanone were evaluated. The yeast reductase YOL151W (GenBank locus tag) exhibited the highest level of activity and exclusively generated the (S)-alcohol. Recombinant YOL151W was pu ...
DOI:
10.1007/s00253-010-2442-5

12. Beyond antibiotic resistance: integrating conjugative elements of the SXT/R391 family that encode novel diguanylate cyclases participate to c-di-GMP signalling in Vibrio cholerae

Author:
Bordeleau, Eric; Brouillette, Eric; Robichaud, Nathaniel; Burrus, Vincent
Source:
Environmental microbiology 2010 v.12 no.2 pp. 510-523
ISSN:
1462-2912
Subject:
Vibrio cholerae; active sites; amino acids; antibiotic resistance; aquatic environment; bacterial motility; biofilm; enzyme activity; enzymes; exopolysaccharides; gene expression; genes; guanosine triphosphate; hosts; phenotype; phosphorylation; second messengers; virulence; Asia; Central America
Abstract:
... In Vibrio cholerae, the second messenger bis-(3′-5′)-cyclic dimeric guanosine monophosphate (c-di-GMP) increases exopolysaccharides production and biofilm formation and decreases virulence and motility. As such, c-di-GMP is considered an important player in the transition from the host to persistence in the environment. c-di-GMP level is regulated through a complex network of more than 60 chromoso ...
DOI:
10.1111/j.1462-2920.2009.02094.x
PubMed:
19888998

13. Biochemical comparison of two proteolytic enzymes from Carica candamarcensis: Structural motifs underlying resistance to cystatin inhibition

Author:
Gomes, Marco Tulio R.; Ribeiro, Henrique A.; Lopes, Miriam T.P.; Guzman, Fanny; Salas, Carlos E.
Source:
Phytochemistry 2010 v.71 no.5-6 pp. 524-530
ISSN:
0031-9422
Subject:
Carica papaya; molecular models; arginine; enzyme inhibition; amino acid sequences; active sites; enzyme kinetics; structure-activity relationships; Vasconcellea pubescens; isozymes; cysteine proteinases
Abstract:
... The lattices of Carica candamarcensis and Carica papaya, members of the Caricaceae family, contain isoforms of cysteine proteinases that help protect these plants against injury. In a prior study, we fractionated 14 discrete proteinaceous components from C. candamarcensis, two of them displaying mitogenic activity in mammalian cells. In this study, we compared the kinetic parameters of one of the ...
DOI:
10.1016/j.phytochem.2009.12.018
PubMed:
20116077

14. Bioinspired synthesis of well faceted CuI nanostructures and evaluation of their catalytic performance for coupling reactions

Author:
Shuyan Gao; Zhengdao Li; Xiaoxia Jia; Kai Jiang; Haibo Zeng
Source:
Green chemistry 2010 v.12 no.8 pp. 1442-1447
ISSN:
1463-9270
Subject:
active sites; ambient temperature; aqueous solutions; benzylamines; catalysts; catalytic activity; green chemistry; iodobenzenes; nanocrystals; surface area
Abstract:
... Well faceted CuI, truncated tetrahedral nanocrystals, were fabricated by an ascorbic acid-assisted green strategy at room temperature in a short period of time. A possible growth mechanism of such highly faceted nanostructures based on typical biomolecule-crystal interactions in aqueous solution is tentatively proposed. The large surface area and rich exposed active sites are expected to endow suc ...
DOI:
10.1039/c0gc00046a

15. C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli

Author:
Ribitsch, D.; Karl, W.; Birner-Gruenberger, R.; Gruber, K.; Eiteljoerg, I.; Remler, P.; Wieland, S.; Siegert, P.; Maurer, K.H.; Schwab, H.
Source:
Journal of biotechnology 2010 v.150 no.3 pp. 408-416
ISSN:
0168-1656
Subject:
Escherichia coli; Stenotrophomonas maltophilia; Xanthomonas campestris; active sites; amino acids; biotechnology; hosts; insulin; metagenomics; models; pH; screening; serine proteinases; washing
Abstract:
... Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli a ...
DOI:
10.1016/j.jbiotec.2010.09.947

16. CE coupled to MALDI with novel covalently coated capillaries

Author:
Bachmann, Stefan; Vallant, Rainer; Bakry, Rania; Huck, Christian W.; Corradini, Danilo; Bonn, Günther K.
Source:
Electrophoresis 2010 v.31 no.4 pp. 618-629
ISSN:
0173-0835
Subject:
active sites; adsorption; coatings; computer software; databases; electrophoresis; fullerene; ions; pH; peptides; polystyrenes; proteins; silica; synergism
Abstract:
... CE offers the advantage of flexibility and method development options. It excels in the area of separation of ions, chiral, polar and biological compounds (especially proteins and peptides). Masking the active sites on the inner surface of a bare fused silica capillary wall is often necessary for CE separations of basic compounds, proteins and peptides. The use of capillary surface coating is one ...
DOI:
10.1002/elps.200900507

17. Carbohydrate binding sites in Candida albicans exo-β-1,3-glucanase and the role of the Phe-Phe 'clamp' at the active site entrance

Author:
Patrick, Wayne M.; Nakatani, Yoshio; Cutfield, Susan M.; Sharpe, Miriam L.; Ramsay, Rochelle J.; Cutfield, John F.
Source:
FEBS journal 2010 v.277 no.21 pp. 4549-4561
ISSN:
1742-464X
Subject:
Candida albicans; active sites; binding sites; carbohydrate binding; cell walls; crystallography; glucan 1,3-beta-glucosidase; glucans; glucose; mutants; mutation; phenylalanine; tryptophan; tyrosine
Abstract:
... Candida albicans exo-β-1,3-glucanase (Exg; EC 3.2.1.58) is implicated in cell wall β- d-glucan remodelling through its glucosyl hydrolase and/or transglucosylase activities. A pair of antiparallel phenylalanyl residues (F144 and F258) flank the entrance to the active site pocket. Various Exg mutants were studied using steady-state kinetics and crystallography aiming to understand the roles played ...
DOI:
10.1111/j.1742-4658.2010.07869.x
PubMed:
20875088

18. Catalytic reaction mechanism of Pseudomonas stutzeri l-rhamnose isomerase deduced from X-ray structures

Author:
Yoshida, Hiromi; Yamaji, Masatsugu; Ishii, Tomohiko; Izumori, Ken; Kamitori, Shigehiro
Source:
FEBS journal 2010 v.277 no.4 pp. 1045-1057
ISSN:
1742-464X
Subject:
Pseudomonas stutzeri; X-radiation; active sites; allose; isomerization; models; mutants; psicose; rhamnose; substrate specificity
Abstract:
... l-Rhamnose isomerase ( l-RhI) catalyzes the reversible isomerization of l-rhamnose to l-rhamnulose. Pseudomonas stutzeri l-RhI, with a broad substrate specificity, can catalyze not only the isomerization of l-rhamnose, but also that between d-allose and d-psicose. For the aldose-ketose isomerization by l-RhI, a metal-mediated hydride-shift mechanism has been proposed, but the catalytic mechanism i ...
DOI:
10.1111/j.1742-4658.2009.07548.x

19. Catalytically active membrane-distal phosphatase domain of receptor protein-tyrosine phosphatase α is required for Src activation

Author:
Vacaru, Andrei M.; den Hertog, Jeroen
Source:
FEBS journal 2010 v.277 no.6 pp. 1562-1570
ISSN:
1742-464X
Subject:
active sites; catalytic activity; cysteine; humans; mutants; mutation; patients; phosphorylation; serine; transmembrane proteins
Abstract:
... Receptor protein-tyrosine phosphatase α (RPTPα) is a transmembrane protein with tandem cytoplasmic phosphatase domains. Most of the catalytic activity is contained by the membrane-proximal catalytic domain (D1). We found a spontaneous Arg554 to His mutation in the pTyr recognition loop of the membrane-distal phosphatase domain (D2) of a human patient. This mutation was not linked to the disease. H ...
DOI:
10.1111/j.1742-4658.2010.07584.x

20. Characterisation of cathepsin B-like cysteine protease of Lepeophtheirus salmonis

Author:
Cunningham, Eleanor; McCarthy, Elaine; Copley, Lorraine; Jackson, Dave; Johnson, David; Dalton, John P.; Mulcahy, Grace
Source:
Aquaculture 2010 v.310 no.1-2 pp. 38-42
ISSN:
0044-8486
Subject:
Salmo salar; ectoparasites; complementary DNA; humans; histidine; Lepeophtheirus salmonis; amino acid sequences; cathepsin B; enzymatic hydrolysis; pH; nucleotide sequences; enzyme kinetics; active sites; temperature; enzyme activity; sequence homology; females; adults
Abstract:
... Lepeophtheirus salmonis, otherwise known as the salmon louse, is an ectoparasite that feeds on Salmo salar (Atlantic salmon), causing large losses to the aquaculture industry. The parasite feeds on the skin and mucus tissues of its host causing extensive damage and bleeding. A cDNA clone encoding a cathepsin B cysteine protease was isolated from adult female L. salmonis (LsCtB). The nucleotide seq ...
DOI:
10.1016/j.aquaculture.2010.10.013

21. Characterization of Xyn10A, a highly active xylanase from the human gut bacterium Bacteroides xylanisolvens XB1A

Author:
Mirande, Caroline; Mosoni, Pascale; Béra-Maillet, Christel; Bernalier-Donadille, Annick; Forano, Evelyne
Source:
Applied microbiology and biotechnology 2010 v.87 no.6 pp. 2097-2105
ISSN:
0175-7598
Subject:
Bacteroides; Escherichia coli; active sites; genes; glucose; humans; oats; pH; phylogeny; signal peptide; temperature; wheat; xylanases
Abstract:
... A xylanase gene xyn10A was isolated from the human gut bacterium Bacteroides xylanisolvens XB1A and the gene product was characterized. Xyn10A is a 40-kDa xylanase composed of a glycoside hydrolase family 10 catalytic domain with a signal peptide. A recombinant His-tagged Xyn10A was produced in Escherichia coli and purified. It was active on oat spelt and birchwood xylans and on wheat arabinoxylan ...
DOI:
10.1007/s00253-010-2694-0
PubMed:
20532756

22. Characterization of the family GH54 α-l-arabinofuranosidases in Penicillium funiculosum, including a novel protein bearing a cellulose-binding domain

Author:
Guais, Olivier; Tourrasse, Olivier; Dourdoigne, Marion; Parrou, Jean Luc; Francois, Jean Marie
Source:
Applied microbiology and biotechnology 2010 v.87 no.3 pp. 1007-1021
ISSN:
0175-7598
Subject:
Talaromyces funiculosus; active sites; alpha-N-arabinofuranosidase; amino acids; arabinose; arabinoxylan; cellulose; fermentation; fungi; genes; pH; proteins; sequence analysis; soil; temperature; thermal stability
Abstract:
... The soil deuteromycete Penicillium funiculosum is characterized by its remarkable capacity to produce a wide variety of cellulolytic and hemicellulolytic enzymes. In the course of the genome sequencing of this industrial fungus, four different genes encoding glycosyl hydrolase family 54 (GH54)22 α-l-arabinofuranosidases were identified. Three of them termed PfabfB1, PfabfB3, and PfabfB4 were highl ...
DOI:
10.1007/s00253-010-2532-4

23. Characterization of two cotton (Gossypium hirsutum L) invertase genes

Author:
Taliercio, Earl; Scheffler, Jodi; Scheffler, Brian
Source:
Molecular biology reports 2010 v.37 no.8 pp. 3915-3920
ISSN:
0301-4851
Subject:
cotton; microsatellite repeats; Gossypium hirsutum; developmental stages; expressed sequence tags; peptides; starch; gene expression; vacuoles; beta-fructofuranosidase; active sites; exons; roots; introns
Abstract:
... Two cotton vacuolar-invertase genes were identified and sequenced. Both genes had seven exons, including an unusually small second exon typical of acid invertases. These genes encode peptides with many features shared by acid invertases from other species including, leader sequences that probably target the peptide to the vacuole, active site motifs and substrate binding motifs. Expression analyse ...
DOI:
10.1007/s11033-010-0048-8

24. Chemical models and their mechanistic implications for the transformation of 6-cyanouridine 5′-monophosphate catalyzed by orotidine 5′-monophosphate decarboxylase

Author:
Yuen-Jen Wu; Chen-Chieh Liao; Cheng-Hung Jen; Yu-Chiao Shih; Tun-Cheng Chien
Source:
Chemical communications 2010 v.46 no.26 pp. 4821-4823
ISSN:
1364-548X
Subject:
active sites; barbiturates; catalytic activity; chemical communication; chemical reactions; enzymes; models
Abstract:
... The reactions of 6-cyano-1,3-dimethyluracil have been studied as chemical models to illustrate the mechanism for the transformation of 6-cyanouridine 5′-monophosphate (6-CN-UMP) to barbiturate ribonucleoside 5′-monophosphate (BMP) catalyzed by orotidine 5′-monophosphate decarboxylase (ODCase). The results suggest that the Asp residue in the ODCase active site plays the role of a general base in th ...
DOI:
10.1039/c001865a

25. Chitinase of Bacillus licheniformis from oyster shell as a probe to detect chitin in marine shells

Author:
Islam, Shah Md. Asraful; Cho, Kye Man; Hong, Sun Joo; Math, Renukaradhya K.; Kim, Jong Min; Yun, Myoung Geun; Cho, Ji Joong; Heo, Jae Young; Lee, Young Han; Kim, Hoon; Yun, Han Dae
Source:
Applied microbiology and biotechnology 2010 v.86 no.1 pp. 119-129
ISSN:
0175-7598
Subject:
Bacillus licheniformis; Chamelea; Crassostrea gigas; Haliotis discus; abalone; active sites; bacteria; chitin; chitinase; clams; fibronectins; fluorescence; genes; manganese; oysters; pH; shell (molluscs); shelling; wastes
Abstract:
... Bacillus licheniformis CBFOS-03 is a chitinase producing bacteria isolated from oyster (Crassostrea gigas) shell waste. We have cloned and expressed the chi18B gene of B. licheniformis CBFOS-03, which encodes a glycohydrolase family 18 chitinase (GH18). Chi18B is a predicted 598 amino acid protein that consists of a catalytic domain (GH18), a fibronectin type III domain (Fn3), and a chitin binding ...
DOI:
10.1007/s00253-009-2215-1
PubMed:
19756579

26. Cloning and characterisation of two CTR1-like genes in Cucurbita pepo: regulation of their expression during male and female flower development

Author:
Manzano, Susana; Martínez, Cecilia; Gómez, Pedro; Garrido, Dolores; Jamilena, Manuel
Source:
Sexual plant reproduction 2010 v.23 no.4 pp. 301-313
ISSN:
0934-0882
Subject:
Arabidopsis; Cucurbita pepo; active sites; consensus sequence; ethylene; ethylene production; female flowers; females; flowering; gene expression regulation; genes; leaves; male flowers; males; mechanism of action; phosphotransferases (kinases); plant organs; roots; seedlings; shoots; tomatoes
Abstract:
... Ethylene is an essential regulator of flower development in Cucurbita pepo, controlling the sexual expression, and the differentiation and maturation of floral organs. To study the action mechanism of ethylene during the male and female flower development, we have identified two CTR1 homologues from C. pepo, CpCTR1 and CpCTR2, and analysed their expressions during female and male flower developmen ...
DOI:
10.1007/s00497-010-0140-1

27. Cloning and characterization of a ribitol dehydrogenase from Zymomonas mobilis

Author:
Moon, Hee-Jung; Tiwari, Manish; Jeya, Marimuthu; Lee, Jung-Kul
Source:
Applied microbiology and biotechnology 2010 v.87 no.1 pp. 205-214
ISSN:
0175-7598
Subject:
DNA; Escherichia coli; NAD (coenzyme); NADP (coenzyme); Zymomonas mobilis; active sites; affinity chromatography; amino acids; gel chromatography; gels; gene overexpression; genes; glutathione transferase; models; molecular weight; open reading frames; pH; polypeptides; sequence analysis; sodium dodecyl sulfate; temperature
Abstract:
... Ribitol dehydrogenase (RDH) catalyzes the conversion of ribitol to d-ribulose. A novel RDH gene was cloned from Zymomonas mobilis subsp. mobilis ZM4 and overexpressed in Escherichia coli BL21(DE3). DNA sequence analysis revealed an open reading frame of 795 bp, capable of encoding a polypeptide of 266 amino acid residues with a calculated molecular mass of 28,426 Da. The gene was overexpressed in ...
DOI:
10.1007/s00253-010-2444-3
PubMed:
20127234

28. Cloning and expression of a mureinolytic enzyme from the mycobacteriophage TM4

Author:
Henry, Marine; Begley, Máire; Neve, Horst; Maher, Fiona; Ross, Reynolds Paul; McAuliffe, Olivia; Coffey, Aidan; O'Mahony, Jim M.
Source:
FEMS microbiology letters 2010 v.311 no.2 pp. 126-132
ISSN:
0378-1097
Subject:
Escherichia coli; active sites; amidase; chloroform; genes; mycobacteriophage; peptidoglycans; polyacrylamide gel electrophoresis; sodium dodecyl sulfate
Abstract:
... In this study, we describe the characterization, cloning, expression and purification of the lysin A gene of the mycobacteriophage TM4. The gene TM4_gp29 (gp29) is a 1644-bp gene that codes for a 58.6-kDa protein and contains peptidoglycan recognition protein, Zn-binding and amidase catalytic domains. The gene was cloned into Escherichia coli using the 'His-Tag' pQE60 vector. After affinity chroma ...
DOI:
10.1111/j.1574-6968.2010.02080.x
PubMed:
20727013

29. Cloning of the RNase H genes from a metagenomic DNA library: identification of a new type 1 RNase H without a typical active-site motif

Author:
Kanaya, E.; Sakabe, T.; Nguyen, N.T.; Koikeda, S.; Koga, Y.; Takano, K.; Kanaya, S.
Source:
Journal of applied microbiology 2010 v.109 no.3 pp. 974-983
ISSN:
1364-5072
Subject:
DNA; DNA fragmentation; DNA libraries; Escherichia coli; active sites; amino acid sequences; bacteria; composts; databases; genes; metagenomics; mutants; nucleotide sequences; phenotype; ribonucleases; screening; sequence homology
Abstract:
... The study aimed to combine a metagenomics approach with complementary genetics to identify novel bacterial genes with orthologous functions, with the identification of novel RNase H genes as a test case. A metagenomic DNA library was prepared from leaf-and-branch compost and used to screen for the RNase H genes by their abilities to complement the temperature-sensitive growth phenotype of the rnhA ...
DOI:
10.1111/j.1365-2672.2010.04724.x
PubMed:
20408915

30. Collagenolytic subtilisin-like protease from the deep-sea bacterium Alkalimonas collagenimarina AC40T

Author:
Kurata, Atsushi; Uchimura, Kohsuke; Kobayashi, Tohru; Horikoshi, Koki
Source:
Applied microbiology and biotechnology 2010 v.86 no.2 pp. 589-598
ISSN:
0175-7598
Subject:
EDTA (chelating agent); Escherichia coli; active sites; amino acids; bacteria; casein; collagen; gelatin; genes; open reading frames; proteinases
Abstract:
... A new alkaline protease (AcpII) was purified from a culture of the deep-sea bacterium Alkalimonas collagenimarina AC40T. AcpII degraded collagen three times faster than it degraded casein. The optimal pH was 8.5-9, and the optimal temperature was 45°C for the degradation of collagen. AcpII was completely inhibited by phenylmethylsulfonyl fluoride and partially by EDTA. Cloning and sequencing the g ...
DOI:
10.1007/s00253-009-2324-x
PubMed:
19888572

31. A Comparative Biochemical Characterization of Microbial Transglutaminases: Commercial vs. a Newly Isolated Enzyme from Streptomyces Sp

Author:
Alves Macedo, Juliana; Durães Sette, Lara; Harumi Sato, Hélia
Source:
Food and bioprocess technology 2010 v.3 no.2 pp. 308-314
ISSN:
1935-5130
Subject:
protein-glutamine gamma-glutamyltransferase; Streptomyces; soil bacteria; isozymes; enzyme activity; enzyme stability; active sites; thiols; enzyme kinetics; Brazil
Abstract:
... A new microbial transglutaminase (MTGase or MTG, EC 2.3.2.13) from a Streptomyces sp. strain isolated from Brazilian soil samples was characterized in crude and purified forms. The aim of this work is to provide relevant information about a new transglutaminase and to compare its characteristics with the well-known commercial transglutaminase from Ajinomoto Co. Inc. (Activa® TG-BP). The enzyme fro ...
DOI:
10.1007/s11947-009-0209-8

32. Comparative Protein Modeling, Prediction of Conserved Residue and Active Sites in Cold Resistant Protein Isolated from CRPF₁, A Cold Tolerant Mutant of Pseudomonas fluorescens

Author:
Khan, Mahejibin; Kumar, Amit; Goel, Reeta
Source:
Current microbiology 2010 v.60 no.6 pp. 428-434
ISSN:
0343-8651
Subject:
DNA; Pseudomonas fluorescens; RNA; active sites; binding properties; cold; cold stress; cold tolerance; models; mutants; prediction; proteins
Abstract:
... Proteins interacting with the biological information molecules DNA and RNA play important cellular roles in all organisms. One widespread super family of proteins implicated in such function(s) is cold shock protein (CSP) that contains the cold shock domain (CSD). This work is planned to study the three-dimensional structure, conserved residues, and different active sites in the structure of cold ...
DOI:
10.1007/s00284-009-9560-2

33. Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme

Author:
Böhm, Kerstin; Herter, Thomas; Müller, Jürgen J.; Borriss, Rainer; Heinemann, Udo
Source:
FEBS journal 2010 v.277 no.5 pp. 1284-1296
ISSN:
1742-464X
Subject:
Escherichia coli; Klebsiella; active sites; crystal structure; humans; ions; mutants; phosphates; phytases; phytic acid; polypeptides; rhizosphere; sequence homology; sulfates
Abstract:
... The extracellular phytase of the plant-associated Klebsiella sp. ASR1 is a member of the histidine-acid-phosphatase family and acts primarily as a scavenger of phosphate groups locked in the phytic acid molecule. The Klebsiella enzyme is distinguished from the Escherichia coli phytase AppA by its sequence and phytate degradation pathway. The crystal structure of the phytase from Klebsiella sp. ASR ...
DOI:
10.1111/j.1742-4658.2010.07559.x
PubMed:
20392204

34. Crystal structure of UDP-galactose 4-epimerase-like l-threonine dehydrogenase belonging to the intermediate short-chain dehydrogenase-reductase superfamily

Author:
Yoneda, Kazunari; Sakuraba, Haruhiko; Muraoka, Ikuo; Oikawa, Tadao; Ohshima, Toshihisa
Source:
FEBS journal 2010 v.277 no.24 pp. 5124-5132
ISSN:
1742-464X
Subject:
Escherichia coli; Flavobacterium; NAD (coenzyme); UDP-glucose 4-epimerase; active sites; adenine; binding sites; crystal structure; glycerol; humans; hydrogen; models; psychrophilic bacteria; ribose; sequence homology; threonine; topology
Abstract:
... The crystal structure of a l-threonine dehydrogenase (l-ThrDH; EC 1.1.1.103) from the psychrophilic bacterium Flavobacterium frigidimaris KUC-1, which shows no sequence similarity to conventional l-ThrDHs, was determined in the presence of NAD and a substrate analog, glycerol. The asymmetric unit consisted of two subunits related by a two-fold rotation axis. Each monomer consisted of a Rossmann-fo ...
DOI:
10.1111/j.1742-4658.2010.07916.x

35. Crystal structures of isomaltase from Saccharomyces cerevisiae and in complex with its competitive inhibitor maltose

Author:
Yamamoto, Keizo; Miyake, Hideo; Kusunoki, Masami; Osaki, Shigeyoshi
Source:
FEBS journal 2010 v.277 no.20 pp. 4205-4214
ISSN:
1742-464X
Subject:
Saccharomyces cerevisiae; active sites; crystal structure; drainage water; enzymes; glucose; glycosides; isomaltose; maltose; surface proteins
Abstract:
... The structures of isomaltase from Saccharomyces cerevisiae and in complex with maltose were determined at resolutions of 1.30 and 1.60 Å, respectively. Isomaltase contains three domains, namely, A, B, and C. Domain A consists of the (β/α)₈-barrel common to glycoside hydrolase family 13. However, the folding of domain C is rarely seen in other glycoside hydrolase family 13 enzymes. An electron dens ...
DOI:
10.1111/j.1742-4658.2010.07810.x
PubMed:
20812985

36. Deimination is regulated at multiple levels including auto-deimination of peptidylarginine deiminases

Author:
Méchin, Marie-Claire; Coudane, Fanny; Adoue, Véronique; Arnaud, Jacques; Duplan, Hélène; Charveron, Marie; Schmitt, Anne-Marie; Takahara, Hidenari; Serre, Guy; Simon, Michel
Source:
Cellular and molecular life sciences 2010 v.67 no.9 pp. 1491-1503
ISSN:
1420-682X
Subject:
active sites; amino acids; gene expression regulation; genes; keratinocytes; messenger RNA; proteins; translation (genetics); vitamin D
Abstract:
... Peptidylarginine deiminases (PADs) catalyze deimination, converting arginyl to citrullyl residues. Only three PAD isotypes are detected in the epidermis where they play a crucial role, targeting filaggrin, a key actor for the tissue hydration and barrier functions. Their expression and activation depends on the keratinocyte differentiation state. To investigate this regulation, we used primary ker ...
DOI:
10.1007/s00018-010-0262-5

37. Detection of immunogenic parasite and host‐specific proteins in the sera of active and chronic individuals infected with Toxoplasma gondii

Author:
Yeng, Chen; Osman, Emelia; Mohamed, Zeehaida; Noordin, Rahmah
Source:
Electrophoresis 2010 v.31 no.23-24 pp. 3843-3849
ISSN:
0173-0835
Subject:
Toxoplasma gondii; Western blotting; active sites; blood proteins; blood serum; circulating antigens; electrophoresis; gels; glycoproteins; host specificity; immunocompromised population; mass spectrometry; parasites; pregnant women; toxoplasmosis
Abstract:
... Toxoplasma gondii infection in pregnant women may result in abortion and foetal abnormalities, and may be life‐threatening in immunocompromised hosts. To identify the potential infection markers of this disease, 2‐DE and Western blot methods were employed to study the parasite circulating antigens and host‐specific proteins in the sera of T. gondii‐infected individuals. The comparisons were made b ...
DOI:
10.1002/elps.201000038

38. Diiron species containing a cyclic Pᴾʰ₂Nᴾʰ₂ diphosphine related to the [FeFe]H₂ases active site

Author:
Sondès Lounissi; Jean-François Capon; Frédéric Gloaguen; Fatma Matoussi; François Y. Pétillon; Philippe Schollhammer; Jean Talarmin
Source:
Chemical communications 2010 v.47 no.3 pp. 878-880
ISSN:
1364-548X
Subject:
active sites; chemical communication; chemical compounds; chemical reactions; enzymes
Abstract:
... A new dissymmetrically disubstituted diiron dithiolate species, [Fe₂(CO)₄(κ²-Pᴾʰ₂Nᴾʰ₂)(μ-pdt)] (pdt = S(CH₂)₃S), was prepared by using a flexible cyclic base-containing diphosphine, 1,3,5,7-tetraphenyl 1,5-diaza-3,7-diphosphacyclooctane (Pᴾʰ₂Nᴾʰ₂ = {PhPCH₂NPh}₂). Preliminary investigations of proton and electron transfers on the diiron system have been done. ...
DOI:
10.1039/c0cc03295f

39. Diol dehydratase‐reactivating factor is a reactivase - evidence for multiple turnovers and subunit swapping with diol dehydratase

Author:
Mori, Koichi; Hosokawa, Yasuhiro; Yoshinaga, Toshiyuki; Toraya, Tetsuo
Source:
FEBS journal 2010 v.277 no.23 pp. 4931-4943
ISSN:
1742-464X
Subject:
active sites; adenine; adenosine diphosphate; adenosine triphosphate; amino acid sequences; crystal structure; dissociation; enzyme activation; enzyme inactivation; glycerol; magnesium; metal ions; nucleosides; propanediol dehydratase; vitamin B12
Abstract:
... Adenosylcobalamin‐dependent diol dehydratase (DD) undergoes suicide inactivation by glycerol, one of its physiological substrates, resulting in the irreversible cleavage of the coenzyme Co-C bond. The damaged cofactor remains tightly bound to the active site. The DD‐reactivating factor reactivates the inactivated holoenzyme in the presence of ATP and Mg²⁺ by mediating the exchange of the tightly b ...
DOI:
10.1111/j.1742-4658.2010.07898.x

40. Directing protonation in [FeFe] hydrogenase active site models by modifications in their second coordination sphere

Author:
Salah Ezzaher; Adolf Gogoll; Clemens Bruhn; Sascha Ott
Source:
Chemical communications 2010 v.46 no.31 pp. 5775-5777
ISSN:
1364-548X
Subject:
active sites; chemical bonding; chemical communication; enzymes; iron; models; protonation
Abstract:
... Subtle changes in the second coordination sphere of [Cl₂bdtFe₂(CO)₄(Ph₂P–CH₂–X–CH₂–PPh₂)] (bdt = benzene-1,2-dithiolate, X = NCH₃, NCH₂CF₃, CH₂) that do not influence the electronic character of the Fe₂ center can however direct protonation to three different sites: the N in the bis-phosphane, the Fe–Fe bond or the bdt-S. ...
DOI:
10.1039/c0cc00724b

41. Effect of the C-terminal domains and terminal residues of catalytic domain on enzymatic activity and thermostability of lichenase from Clostridium thermocellum

Author:
Niu, Dong; Zhou, Xu-xia; Yuan, Tao-yan; Lin, Zhi-wei; Ruan, Hui; Li, Wei-fen
Source:
Biotechnology letters 2010 v.32 no.7 pp. 963-967
ISSN:
0141-5492
Subject:
Clostridium thermocellum; Escherichia coli; active sites; enzyme activity; genes; licheninase; thermal stability
Abstract:
... To elucidate the effects of C-terminal domains of LicMB (mature lichenase from Clostridium thermocellum) and terminal residues of LicMB-CD (catalytic domain of LicMB) on the properties of lichenase, a series of truncated genes were constructed and expressed in E. coli. The Thr-Pro box had a positive effect while the dockerin domain had a negative impact on the properties of LicMB. The N-terminal 1 ...
DOI:
10.1007/s10529-010-0241-9
PubMed:
20229062

42. Effects of Site-Directed Mutagenesis of the Loop Residue of the N-Terminal Domain Gly117 of Thermolysin on Its Catalytic Activity

Author:
Evans MENACH; Kiyoshi YASUKAWA; Kuniyo INOUYE
Source:
Bioscience, biotechnology, and biochemistry 2010 v.74 no.12 pp. 2457-2462
ISSN:
1347-6947
Subject:
Escherichia coli; active sites; biotechnology; catalytic activity; hydrolysis; polypeptides; site-directed mutagenesis; thermolysin
Abstract:
... In the N-terminal domain of thermolysin, two polypeptide strands, Asn112-Ala113-Phe114-Trp115 and Ser118-Gln119-Met120-Val121-Tyr122, are connected by a short loop, Asn116-Gly117, to form an anti-parallel β-sheet. The Asn112-Trp115 strand is located in the active site, while the Ser118-Tyr122 strand and the Asn116-Gly117 loop are located outside the active site. In this study, we explored the cata ...
DOI:
10.1271/bbb.100536

43. Effects of catalytic site mutations on active expression of phage fused penicillin acylase

Author:
Shi, Yi-Feng; Soumillion, Patrice; Ueda, Mitsuyoshi
Source:
Journal of biotechnology 2010 v.145 no.2 pp. 139-142
ISSN:
0168-1656
Subject:
DNA libraries; Western blotting; active sites; bacteriophages; benzylpenicillin; biotechnology; coat proteins; enzyme activity; evolution; genes; industry; secretion; signal peptide; site-directed mutagenesis
Abstract:
... Penicillin G acylase (EC 3.5.1.11) is 86-kDa large heterodimeric protein comprising two peptide A 23-kDa and peptide B 62-kDa, produced by intein-mediated auto-splicing of a 92-kDa precursor. Since penicillin G acylase was potentially employed in the preparation of a wide range of semi-synthetic β-lactam antibiotics from acyl side-chain precursors and β-lactam nucleus, directed evolution of penici ...
DOI:
10.1016/j.jbiotec.2009.11.009

44. Emerging roles for WNK kinases in cancer

Author:
Moniz, Sónia; Jordan, Peter
Source:
Cellular and molecular life sciences 2010 v.67 no.8 pp. 1265-1276
ISSN:
1420-682X
Subject:
active sites; apoptosis; carcinogenesis; cell cycle; genes; humans; kidneys; metastasis; neoplasm cells; neoplasms; protein kinases; tissues; transporters
Abstract:
... The subfamily of WNK protein kinases is composed of four human genes and is characterised by a typical sequence variation within the conserved catalytic domain. Although most research has focussed on the role of WNK1, WNK3 and WNK4 in regulating different ion transporters in both the kidney and extrarenal tissues, there is growing evidence for additional roles of WNK kinases in various signalling ...
DOI:
10.1007/s00018-010-0261-6

45. Engineering a family 9 processive endoglucanase from Paenibacillus barcinonensis displaying a novel architecture

Author:
Chiriac, Alina Iulia; Cadena, Edith Marleny; Vidal, Teresa; Torres, Antonio L.; Diaz, Pilar; Javier Pastor, F. I.
Source:
Applied microbiology and biotechnology 2010 v.86 no.4 pp. 1125-1134
ISSN:
0175-7598
Subject:
Paenibacillus; active sites; cellulose; endo-1,4-beta-glucanase; engineering; sugars
Abstract:
... Cel9B from Paenibacillus barcinonensis is a modular endoglucanase with a novel molecular architecture among family 9 enzymes that comprises a catalytic domain (GH9), a family 3c cellulose-binding domain (CBM3c), a fibronectin III-like domain repeat (Fn3₁,₂), and a C-terminal family 3b cellulose-binding domain (CBM3b). A series of truncated derivatives of endoglucanase Cel9B have been constructed a ...
DOI:
10.1007/s00253-009-2350-8
PubMed:
19957081

46. Engineering active sites for enhancing synergy in heterogeneous catalytic oxidations

Author:
James Paterson; Matthew Potter; Enrica Gianotti; Robert Raja
Source:
Chemical communications 2010 v.47 no.1 pp. 517-519
ISSN:
1364-548X
Subject:
active sites; alkenes; aluminum; catalysts; catalytic activity; cobalt; engineering; epoxidation reactions; metal ions; titanium
Abstract:
... The simultaneous isomorphous substitution of Al(iii) and P(v) ions, in an aluminophosphate framework, with redox active Co(iii) and Ti(iv) metal ions, generates highly active single-site heterogeneous catalysts that exhibit considerable synergy, compared to their corresponding monometallic analogues, in the catalytic epoxidation of olefins. ...
DOI:
10.1039/c0cc02341h

47. Engineering of Candida antarctica lipase B for hydrolysis of bulky carboxylic acid esters

Author:
Juhl, P.B.; Doderer, K.; Hollmann, F.; Thum, O.; Pleiss, J.
Source:
Journal of biotechnology 2010 v.150 no.4 pp. 474-480
ISSN:
0168-1656
Subject:
Escherichia coli; Pseudozyma antarctica; active sites; alcohols; biocatalysts; biotechnology; engineering; enzyme substrates; fatty acid esters; hexanoic acid; hydrolysis; screening
Abstract:
... Candida antarctica lipase B (CALB) is a widely used biocatalyst with high activity and specificity for a wide range of primary and secondary alcohols. However, the range of converted carboxylic acids is more narrow and mainly limited to unbranched fatty acids. To further broaden the biotechnological applications of CALB it is of interest to expand the range of converted carboxylic acid and extend ...
DOI:
10.1016/j.jbiotec.2010.09.951

48. Engineering of a novel chimera of superoxide dismutase and Vitreoscilla Hemoglobin for rapid detoxification of reactive oxygen species

Author:
Isarankura-Na-Ayudhya, Chartchalerm; Yainoy, Sakda; Tantimongcolwat, Tanawut; Bülow, Leif; Prachayasittikul, Virapong
Source:
Journal of bioscience and bioengineering 2010 v.110 no.6 pp. 633-637
ISSN:
1389-1723
Subject:
peroxidase; humans; catalase; heme; Vitreoscilla; hydrogen peroxide; superoxide dismutase; hemoglobin; genes; active sites; pH; oxygen
Abstract:
... The genes encoding human manganese superoxide dismutase (MnSOD) and Vitreoscilla hemoglobin (VHb) were fused in-frame to generate a bifunctional enzyme that possessed MnSOD and peroxidase-like activities. At neutral pH, the coupling of the SOD and peroxidase reactions revealed that the bifunctional enzyme exhibited a 2.5 times shorter transient period and a 1.67 times higher reaction rate at stead ...
DOI:
10.1016/j.jbiosc.2010.07.001

49. Engineering of choline oxidase from Arthrobacter nicotianae for potential use as biological bleach in detergents

Author:
Ribitsch, Doris; Winkler, Sonja; Gruber, Karl; Karl, Wolfgang; Wehrschütz-Sigl, Eva; Eiteljörg, Inge; Schratl, Petra; Remler, Peter; Stehr, Regina; Bessler, Cornelius; Mußmann, Nina; Sauter, Kerstin; Maurer, Karl Heinz; Schwab, Helmut
Source:
Applied microbiology and biotechnology 2010 v.87 no.5 pp. 1743-1752
ISSN:
0175-7598
Subject:
Arthrobacter nicotianae; active sites; bleaching agents; choline; choline oxidase; detergents; directed evolution; engineering; enzyme activity; models; mutants; mutation; screening
Abstract:
... In order to engineer the choline oxidase from Arthrobacter nicotianae (An_CodA) for the potential application as biological bleach in detergents, the specific activity of the enzyme toward the synthetic substrate tris-(2-hydroxyethyl)-methylammonium methylsulfate (MTEA) was improved by methods of directed evolution and rational design. The best mutants (up to 520% wt-activity with MTEA) revealed m ...
DOI:
10.1007/s00253-010-2637-9
PubMed:
20467737

50. Enrichment of ligands with molecular dockings and subsequent characterization for human alcohol dehydrogenase 3

Author:
Hellgren, Mikko; Carlsson, Jonas; Östberg, Linus J.; Staab, Claudia A.; Persson, Bengt; Höög, Jan-Olov
Source:
Cellular and molecular life sciences 2010 v.67 no.17 pp. 3005-3015
ISSN:
1420-682X
Subject:
active sites; alcohol dehydrogenase; deoxycholic acid; doxorubicin; fatty acids; glutathione; homeostasis; humans; ligands; nitric oxide; screening
Abstract:
... Alcohol dehydrogenase 3 (ADH3) has been assigned a role in nitric oxide homeostasis due to its function as an S-nitrosoglutathione reductase. As altered S-nitrosoglutathione levels are often associated with disease, compounds that modulate ADH3 activity might be of therapeutic interest. We performed a virtual screening with molecular dockings of more than 40,000 compounds into the active site of h ...
DOI:
10.1007/s00018-010-0370-2
PubMed:
20405162