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You searched for: Publication Year 2010 Remove constraint Publication Year: 2010 Subject amino acids Remove constraint Subject: amino acids Subject term Mucor Remove constraint Subject term: Mucor Text Availability Citation in PubAg Remove constraint Text Availability: Citation in PubAg
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1. Secretory expression and purification of a soluble NADH cytochrome b5 reductase enzyme from Mucor racemosus in Pichia pastoris based on codon usage adaptation

Author:
S. A. Mirzaei; M. Tabatabaei Yazdi; Z. Sepehrizadeh
Source:
Biotechnology letters 2010 v.32 no.11 pp. 1705-1711
ISSN:
0141-5492
Subject:
NAD (coenzyme); alcohol oxidase; Komagataella pastoris; methanol; Mucor racemosus; genome; yeasts; amino acids; culture media; Escherichia coli
Abstract:
... The genome of Mucor racemosus was analyzed to determine the relative levels of codon usage. The codon bias differred from that of Escherichia coli. The active, soluble isoform of NADH cytochrome b5 reductase containing 228 amino acids was successfully overexpressed and secreted using alpha factor in Pichia pastoris under the control of the alcohol oxidase promoter and finally purified. The culture ...
DOI:
10.1007/s10529-010-0348-z
PubMed:
20623315

2. A new β-glucosidase gene from the zygomycete fungus Rhizomucor miehei

Author:
Takó, Miklós; Tóth, Adél; Nagy, László G.; Krisch, Judit; Vágvölgyi, Csaba; Papp, Tamás
Source:
Antonie van Leeuwenhoek 2010 v.97 no.1 pp. 1-10
ISSN:
0003-6072
Subject:
Mucor; proteins; Rhizomucor miehei; plasmids; amino acids; active sites; introns; sequence homology; beta-glucosidase; fungi
Abstract:
... In this study, a β-glucosidase coding gene (bgl) of the zygomycete fungus Rhizomucor miehei has been cloned and characterized. The gene comprises a total of 2,826 bp including the coding sequence of a 717 amino acids length putative protein and 10 introns dispersed in the whole coding region. The putative N-and C-terminal catalytic domains (aa 68 to aa 274 and aa 358-601, respectively) were identi ...
DOI:
10.1007/s10482-009-9382-z