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sequence analysis; animal growth; point mutation; bone metabolism; female reproductive system; animal proteins; genetic improvement; heterozygosity; female fertility; Rasa Aragonesa; ewes; linkage (genetics); ovulation; exons; animal genetics; single nucleotide polymorphism
Abstract:
... Different mutations in the Bone Morphogenetic Protein 15 (BMP15) and the Growth Differentiation Factor 9 (GDF9) genes cause increased ovulation rate and infertility in a dosage-sensitive manner in sheep. They cause increased ovulation rate and twin and triplet births in heterozygotes, and complete primary ovarian failure in homozygotes resulting in total infertility. We are here presenting a novel ...
... The past century has seen animal breeding and genetics evolve and expand from definition and validation of basic population genetics theory to development of selection index theory to today's relatively sophisticated genetic prediction systems enabling industry genetic improvement. The end of the first century of the American Society of Animal Science coincides with the rapid movement of the field ...
... Mortality records of indigenous and upgraded pigs, reared under subtropical hill agro climatic conditions, were collected for the period of 11 years (1993-2003), analyzed and the mortality pattern was correlated with genetic group, age of pigs, climatic factors and causes of death. Results revealed that there was significant (p <0.05) difference in mortality between the indigenous (6.05%) and upgr ...
... Paratuberculosis represents a major problem in farmed ruminants and at the present is considered a potential zoonosis. The disease is caused by Mycobacterium avium subsp. paratuberculosis, and susceptibility to infection is suspected to have a genetic component. Caspase recruitment domain 15 (CARD15) gene encodes for a cytosolic protein implicated in bacterial recognition during innate immunity. C ...
... Developmental Orthopaedic Disease (DOD) in limb joints of horses is frequent and is a common cause of pain and lameness. DOD is a multifactorial disease involving genetics, growth, feeding practices and exercise conditions leading to joint injuries. However, there is no clear understanding of the contribution of each factor. The aim of this cohort study was to assess the adjusted effects of breed ...
... Our previous genetic studies demonstrated that resistance to avian coccidiosis is linked with microsatellite markers LEI0071 and LEI0101 on chromosome 1. In this study, the associations between parameters of resistance to coccidiosis and single nucleotide polymorphisms (SNP) in 3 candidate genes located between LEI0071 and LEI0101 [zyxin, CD4, and tumor necrosis factor receptor super family 1A (TN ...
... Classical scrapie disease is a transmissible spongiform encephalopathy of sheep that is enzootic in the United States. Susceptibility of sheep to classical scrapie is linked to single nucleotide polymorphisms in the prion protein gene (PRNP), forming the basis for genetic testing strategies used by national efforts to eradicate scrapie. Such efforts are occasionally hampered by inconclusive result ...
... Population structure, performance testing and breeding scheme of the sire breed Piétrain in Bavaria were analyzed as a basis for further optimization studies of the breeding programme. To evaluate the current breeding programme, genetic trends and effective population size were estimated. Four data sets were used which contained breeding animals born between 1981 and 2005, estimated breeding value ...
... CD81, also known as the target of an antiproliferative antibody 1 (TAPA-1) in human, is a member of tetraspanin integral membrane protein family. This protein plays many important roles in immune and other physiological functions. In this report, we characterized and analyzed expression of the channel catfish CD81 transcript. The full-length of channel catfish CD81 cDNA comprised of 1130 nucleotid ...
... To increase our understanding of imprinted genes in swine, we carried out a comprehensive analysis of this gene family using two complementary approaches: expression and phenotypic profiling of parthenogenetic fetuses, and analysis of imprinting by pyrosequencing. The parthenote placenta and fetus were smaller than those of controls but had no obvious morphological differences at Day 28 of gestati ...
... NALP5, also known as MATER (maternal antigen that embryos require), is an oocyte-specific maternal effect gene required for early embryonic development. Because of the specificity of NALP5 expression, and its role in female fertility, NALP5 is an interesting candidate gene for economically important female reproductive traits. Here we describe the chromosomal assignment of the porcine NALP5 gene t ...
... 1. The objective of this study was to investigate the effect of ADSL gene, GARS-AIRS-GART gene and their combination genotype on inosine monophosphate content (IMP) in chicken. 2. The chicken breeds used for this study were Recessive White chicken (RW, Jiang-13 strain of white Plymouth Rock) and preserved population of 4 Chinese native chicken breeds, including Silkies, Baier, Tibetan and Xiaoshan ...
... High genetic diversity of East Asian village dogs has recently been used to argue for an East Asian origin of the domestic dog. However, global village dog genetic diversity and the extent to which semiferal village dogs represent distinct, indigenous populations instead of admixtures of various dog breeds has not been quantified. Understanding these issues is critical to properly reconstructing t ...
... Currently, genomic evaluations use multiple-step procedures, which are prone to biases and errors. A single-step procedure may be applicable when genomic predictions can be obtained by modifying the numerator relationship matrix A to H = A + AΔ, where AΔ includes deviations from expected relationships. However, the traditional mixed model equations require H⁻¹, which is usually difficult to obtain ...
Antonio M. Ramos; Richard P. M. A. Crooijmans; Nabeel A. Affara; Andreia J. Amaral; Alan L. Archibald; Jonathan E. Beever; Christian Bendixen; Carol Churcher; Richard Clark; Patrick Dehais; Mark S. Hansen; Jakob Hedegaard; Zhi-Liang Hu; Hindrik H. Kerstens; Andy S. Law; Hendrik-Jan Megens; Denis Milan; Danny J. Nonneman; Gary A. Rohrer; Max F. Rothschild; Tim P. L. Smith; Robert D. Schnabel; Curt P. Van Tassell; Jeremy F. Taylor; Ralph T. Wiedmann; Lawrence B. Schook; Martien A. M. Groenen; Laszlo Orban
... The dissection of complex traits of economic importance to the pig industry requires the availability of a significant number of genetic markers, such as single nucleotide polymorphisms (SNPs). This study was conducted to discover several hundreds of thousands of porcine SNPs using next generation sequencing technologies and use these SNPs, as well as others from different public sources, to desig ...
genetic merit; prediction; ease of birth; calving; dairy cattle; genes; genomics; additive gene effects; accuracy; animal genetics; single nucleotide polymorphism
Abstract:
... Genetic effects for many dairy traits and for total economic merit are evenly distributed across all chromosomes. A high-density scan using 38,416 single nucleotide polymorphism markers for 5,285 bulls confirmed 2 previously known major genes on Bos taurus autosomes (BTA) 6 and 14 but revealed few other large effects. Markers on BTA18 had the largest effects on calving ease, several conformation t ...
breed differences; genetic polymorphism; cryopreservation; acrosome reaction; selection criteria; fertilization (reproduction); codons; male fertility; genotype; rams; genetic variation; in vitro fertilization; embryogenesis; spermatozoa; sheep breeds; animal genetics; prions; Portugal
Abstract:
... Transgenic knockout of the gene encoding the prion-like protein Doppel leads to male infertility in mice. The precise role of Doppel in male fertility is still unclear, but sperm from Doppel-deficient mice appear to be unable to undergo the normal acrosome reaction necessary to penetrate the zona pellucida of the oocyte. The objective of this study was to characterize Doppel (Prnd) gene polymorphi ...
... Seven test-day models with different ways of accounting for the effect of pregnancy on production traits were compared by their residual variance, rank correlations of estimated breeding values of bulls and cows and number of nonpregnant cows in the top 500 for milk yield and milk persistency. Data were 22,546,696 first-parity test-day milk, fat, and protein yields and somatic cell score records o ...
... Recently, selective breeding was proposed as a means of changing the fatty acid composition of milk to improve its nutritional quality. Before implementing such breeding objectives, effects on other economically important traits should be investigated. The objectives of this study were to examine 1) the effect of milk fat composition, and 2) the effect of polymorphisms of DGAT1 and SCD1 genes on f ...
estimation; animal genetics; racing performance; Thoroughbred; racehorses; long term effects; animal age; mathematical models; heritability; Iran
Abstract:
... The aim of this study was to estimate the genetic parameters of racing performance and investigate the fixed effects that influence the performance of Thoroughbred race horses. The data included the performance of two, three and four year old horses raced in flat races and consisted of 10,800 time records and 10,912 ranking value. Two criteria were used in order to analyze the performance: racing ...
... Microarrays have become an important research tool for life science researchers. Expression microarrays are capable of profiling the gene expression pattern of tens of thousands of genes in a single experiment. It appears to be the platform of choice for parallel gene expression profiling. Various equine-specific gene expression microarrays have been generated and used. However, homologous microar ...
... The cutaneous barrier contains small, cationic antimicrobial peptides that participate in the innate immunity against a wide variety of pathogens. Despite their immune importance, knowledge of canine defensins and their expression is limited primarily to testicular tissue and their relation to coat colour. Studies have shown that the absence of these antimicrobial peptides contribute to increased ...
... In eukaryotic cells, the major protein constituents of the chromatin are histones, which can be divided into five classes, identified as H1, H2A, H2B, H3 and H4. During normal spermatogenesis, a testis-specific H1t is expressed in primary spermatocytes and believed to facilitate histone to protamine exchanges during spermiogenesis. In equine testes we detected the H1 protein at 22kDa by western bl ...
... Ten Greek sheep breeds were analysed at 28 microsatellite markers in order to estimate their genetic diversity and differentiation. This study aims to provide information on the genetic structure of the breeds analysed and the ancestral populations, and give indications and proposals for the conservation strategies. The breeds included were the local sheep breeds raised in different regions of Gre ...
... In this study a total of 6499 records were obtained for fibre diameter (FD) and coefficient of variation of FD (CV), 3283 records for greasy fleece weight (GFW), staple length (SL) and shearing interval (SI) and 1802 records of textile value index (TV) obtained from an experimental herd of alpacas exploited in the Peruvian Altiplano. The estimated heritabilities were: 0.412±0.015 (FD), 0.321±0.013 ...
... The objective of this study was to evaluate genetic and non-genetic factors influencing characteristics of young buck semen production using a multivariate model that takes into account the longitudinal structure of data. Data were collected from 1989 to 2002 at two French A.I. centres. The data corresponded to 13151 and 9206 ejaculates of 758 Alpine and 535 Saanen bucks respectively, collected at ...
... Minerals found in milk, such as Se, Ca, K, Zn, Mg, and P, contribute to several vital physiological processes. The aim of this study was to quantify the genetic variation in levels of Se, Ca, K, Zn, Mg, and P in milk and to quantify the between-herd variation in the levels of these minerals in milk. One morning milk sample from each of 1,860 Dutch Holstein-Friesian cows from 388 commercial herds i ...
... Variance components and further phenotypic and genetic parameters for reproduction traits including litter size at birth (LSB) and at weaning (LSW), gestation period (GP) and litter weight at birth (LWB) were estimated by use of the MTGSAM procedure fitting two-trait animal model (y = X b + Z a a + Z P P + e ) in Boer dams. The calculations were based on 1205 parities of 435 Boer dams at Boer Goat ...
... The knowledge of the genetic relationship and admixture among neighbouring populations is crucial for conservation efforts. The aim of this study was to analyse the genetic diversity of five Italian sheep breeds (Appenninica, Garfagnina Bianca, Massese, Pomarancina and Zerasca) using a panel of 24 microsatellite markers. Blood samples from 226 individuals belonging to the aforementioned population ...
... The aim of this study was to evaluate the genetic connectedness between seven European populations of show jumping horses. Data on stallions bred for jumping and their pedigree up to three generations were provided by seven countries, including 6317 stallions and 22324 different horses. Four different methods were used to estimate the genetic connectedness. Common stallions between countries varie ...
... The first breeding value for udder health of a bull is based on the performance of his daughters in their first lactation. However, clinical mastitis (CM) is not a problem in first lactation only. Therefore, the objective of this study was to estimate genetic parameters for CM and somatic cell count (SCC) for the first three lactations of Dutch Holstein cattle. Data from 250 Dutch herds recording ...
... STATs are a group of transcription factors that mediate actions of a variety of peptide hormones and cytokines within target cells (for example, prolactin and growth hormone). Therefore, STAT5A gene is a candidate marker for quantitative traits in farm animals with respect to milk production traits. In this study the STAT5A/AvaI polymorphism was investigated with PCR-RFLP in a sample of 233 Italia ...
... The genetic variability and presence of population substructures in 4 native Northern Italian sheep breeds, Alpagota, Brogna, Foza, and Lamon, undergoing in situ conservation, and 1 widespread Italian breed, Bergamasca, were studied by investigating 19 microsatellite markers. The breeds showed considerable genetic variability in terms of number of alleles and heterozygosity, with the exception of ...
... Aggression when pigs are mixed into new social groups has negative impacts on welfare and production. Aggressive behaviour is moderately heritable and could be reduced by genetic selection. The possible wider impacts of selection for reduced aggressiveness on handling traits and activity in the home pen were investigated using 1663 male and female pedigree pigs (898 purebred Yorkshire and 765 York ...
... Thoroughbred horses have been selected for exceptional racing performance resulting in system-wide structural and functional adaptations contributing to elite athletic phenotypes. Because selection has been recent and intense in a closed population that stems from a small number of founder animals Thoroughbreds represent a unique population within which to identify genomic contributions to exercis ...
... Array-based comparative genomics hybridization (aCGH) has gained prevalence as an effective technique for measuring structural variations in the genome. Copy-number variations (CNVs) form a large source of genomic structural variation, but it is not known whether phenotypic differences between intra-species groups, such as divergent human populations, or breeds of a domestic animal, can be attribu ...
... Mutations I48L, I54V, R86Q, V137I, I492M, and T548A were identified previously in BmAChE3, a gene encoding acetylcholinesterase, from the organophosphate (OP) acaricide-resistant San Román strain of Rhipicephalus (Boophilus) microplus. Recombinant BmAChE3 acetylcholinesterase containing the R86Q mutation was shown to exhibit nearly 20-fold reduction in the rate of phosphorylation by paraoxon relat ...
breed differences; geographical variation; population characteristics; risk factors; genetic variation; sheep breeds; animal genetics; United Kingdom
Abstract:
... This study addresses the potential risk to the genetic resources of UK sheep breeds from endemism. Twelve native breeds are analysed that exist in significant numbers and continue to be commercially farmed livestock. The breeds were selected to represent regions of the British Isles: North of England hill breeds (Herdwick, Rough Fell, Lonk, Dalesbred), South of England breeds (Southdown, Romney, D ...
... Three hundred and thirteen Estrela mountain dogs were examined for hip dysplasia (HD) using the standard ventrodorsal hip extended view, and graded into five categories (A, B, C, D and E) using the Fédération Cynologique Internationale’s (FCI) scoring system. The Ortolani method was performed to evaluate hip joint laxity. Pedigree information was obtained from the Portuguese Kennel Club and the ge ...
... Cathelicidins are important components of the innate immune system and have been identified in skin and epithelia of a range of mammals. In this study molecular techniques, including RACE-PCR, were used to identify the full cDNA sequence of a cathelicidin gene, MaeuCath8, from the Australian marsupial, the tammar wallaby, Macropus eugenii. This cathelicidin was not homologous to other such genes p ...
... The existence of specific messenger RNA remnants contained within freshly ejaculated spermatozoa was described in several species. Those investigations, using high-throughput techniques to screen the population of transcripts in ejaculated spermatozoa, were limited to the probes which mostly derived from nucleic acids of testicular tissues of either human or mice. The objective of this study was t ...
... The ring finger protein 4 gene (RNF4), which might play a role in fetal germ cell development as well as in oocyte and granulosa cell maturation, was one of the potential candidate genes for reproductive traits. In the present work, we isolated the complete coding sequence of porcine RNF4 gene, identified a single nucleotide polymorphism (SNP: T/C) in intron5, and developed a PCR-SacII-RFLP genoty ...
... As part of a functional genomic study of scrapie pathogenesis we have generated a complex non-redundant microarray from several tissues by the selection of unique clones and the incremental augmentation of the expressed sequence tag sets. The base set of about 15,000 expressed sequence tags had minimum redundancy and originated from bovine brain. The aim was to augment this set with clones from sc ...
... Dairy sheep have been farmed traditionally in the Mediterranean basin in southern Europe, central Europe, eastern Europe, and in Near East countries. Currently, dairy sheep farming systems vary from extensive to intensive according to the economic relevance of the production chain and the specific environment and breed. Modern breeding programs were conceived in the 1960s. The most efficient selec ...
Rotylenchulus reniformis; animal genetics; population genetics; genetic variation; microsatellite repeats; DNA fingerprinting; genotype; plant parasitic nematodes; Mississippi
Abstract:
... Rotylenchulus reniformis is the predominant parasitic nematode of cotton in the Mid South area of the United States. Although variable levels of infection and morphological differences have been reported for this nematode, genetic variability has been more elusive. We developed microsatellite-enriched libraries for R. reniformis, produced 1152 clones, assembled 694 contigs, detected 783 simple seq ...
chickens; interspecific variation; epitopes; protein structure; protein synthesis; recombinant proteins; Western blotting; monoclonal antibodies; genes; mammals; terminal repeat sequences; antigenic variation; animal genetics; prions
Abstract:
... We obtained seven monoclonal antibodies (mAbs) against chicken cellular prion protein (ChPrPC) by immunizing BALB/c mice with recombinant prion protein (rChPrP). Of the seven mAbs, two mAbs (6 and 26) could recognize rChPrP, but not ChPrPC, in chicken brain lysate via Western blot (WB) analysis. Three C-terminal linear epitopes (AAANQTEVEM, RWWS and SPVPQD) were identified in ChPrP amino acids by ...
... The present study was carried out in the Animal Genetics Division, Indian Veterinary Research Institute. The cDNA for CD14 gene of goat was amplified for the first time using PCR with ATGGTCTGCGTGCCCTACCTG as forward primer and GGAGCCCGAGGCTTCGCGTAA as reverse primer. The PCR product of 1122bp was eluted, purified, cloned and sequenced by automated sequencer (ABI prism) using dideoxy chain termina ...
... The duration of nocturnal melatonin secretion reaches its minimum in summer, a physiological event that is likely related with the diminished sow fertility and delayed puberty typically observed in this season. Melatonin exerts its function by binding two different receptors named as MTNR1A and MTNR1B. Interestingly, the MTNR1A gene is located on a chromosome SSC17 region where QTL for prolificacy ...
... Follicle stimulating hormone (FSH) is a pituitary gonadotropin that plays a key role in the regulation of gonadal function in mammal. The gonadotropic hormones are composed of two subunits, the common alpha subunit and the hormone-specific beta subunit, which determine the binding to specific receptors and induction of biological response. The aim of this study was to investigate the polymorphism ...
... The Black Bengal is a prolific goat breed in India. Natural mutations in prolific sheep breeds have shown that the transforming growth factor beta (TGF-β) super family ligands such as growth differentiation factor 9 (GDF9), bone morphogenetic protein 15 (BMP15) and their type I receptor (bone morphogenetic protein receptor, BMPR1B) are crucial for ovulation and as well as for increasing litter siz ...
... The objective of this study was to compare alternative trait definitions and statistical models for genetic evaluation of survival in dairy cattle. Data from the first 5 lactations of 808,750 first-crop daughters of 3,064 Norwegian Red sires were analyzed. Seven sire models were used for genetic analyses: linear and threshold cross-sectional models for binary survival scores from first lactation; ...
... The phenotypic observation of some reproduction traits (e.g., insemination success, interval from lambing to insemination) is the result of environmental and genetic factors acting on 2 individuals: the male and female involved in a mating couple. In animal genetics, the main approach (called additive model) proposed for studying such traits assumes that the phenotype is linked to a purely additiv ...
Barrows, Frederic T.; Bellis, Diane; Krogdahl, Ashild; Silverstein, Jeffrey T.; Herman, Eliot M.; Sealey, Wendy M.; Rust, Michael B.; Gatlin, Delbert M. III
Decker, Jared E.; Pires, J. Chris; Conant, Gavin C.; McKay, Stephanie D.; Heaton, Michael P.; Chen, Kefei; Cooper, Alan; Vilkki, Johanna; Seabury, Christopher M.; Caetano, Alexandre R.; Johnson, Gary S.; Brenneman, Rick A.; Hanotte, Olivier; Eggert, Lori S.; Wiener, Pamela; Kim, Jong-Joo; Kim, Kwan Suk; Sonstegard, Tad S.; Van Tassell, Curt P.; Neibergs, Holly L.; McEwan, John C.; Brauning, Rudiger; Coutinho, Luiz L.; Babar, Masroor E.; Wilson, Gregory A.; McClure, Matthew C.; Rolf, Megan M.; Kim, JaeWoo; Schnabel, Robert D.; Taylor, Jeremy F.
... The Pecorans (higher ruminants) are believed to have rapidly speciated in the Mid-Eocene, resulting in five distinct extant families: Antilocapridae, Giraffidae, Moschidae, Cervidae, and Bovidae. Due to the rapid radiation, the Pecoran phylogeny has proven difficult to resolve, and 11 of the 15 possible rooted phylogenies describing ancestral relationships among the Antilocapridae, Giraffidae, Cer ...
... Biological bases for sexual differences in the brain exist in a wide range of vertebrate species, including chickens. Recently, the dogma of hormonal dependence for the sexual differentiation of the brain has been challenged. We examined whether sexually dimorphic gene expression in the brain precedes gonadal differentiation. Using the Affymetrix GeneChip Chicken Genome Array, we identified 42 fem ...
... Alpha-lactalbumin is a major whey protein found in milk. It influences lactose synthesis by modifying the substrate specificity of galactosyl-transferase, is important to milk synthesis since lactose, an impermeable disaccharide, is the major osmole of milk. The present study was undertaken to detect polymorphism in the full coding region of alpha-lactalbumin at the genetic level and to explore al ...
... Toll like receptors (TLRs) are a class of pattern recognition receptors belonging to the innate immune system. Mutations in the protein coding region of TLRs are associated with altered responsiveness to pathogen-associated molecular patterns (PAMPs). A search was performed for novel mutations in bovine TLR1, TLR2 and TLR4 genes associated with the Mycobacterium avium subsp. paratuberculosis (MAP) ...
... As a member of the POU-domain family, pituitary-specific transcription factor 1 (POU1F1) is responsible for a phenotype of combined pituitary hormone deficiency (CPHD) involving growth hormone (GH), prolactin (PRL) and thyrotropin β subunit (TSHβ). Owing to PRL concentrations associated with wool growth and wool traits, the POU1F1 gene is a potential candidate gene for cashmere traits selection an ...
Sus scrofa; wild boars; animal genetics; population genetics; wildlife diseases; disease control; population distribution; Bayesian theory; simulation models; thematic maps; cluster analysis; Germany
Abstract:
... 1. Spatially explicit Bayesian clustering techniques offer a powerful tool for ecology and wildlife management, as genetic divisions can be correlated with landscape features. We used these methods to analyse the genetic structure of a population of European wild boar Sus scrofa with the aim of identifying effective barriers for disease management units. However, it has been suggested that the met ...
... The present work aimed to determine how the average fibre diameter coefficient of variation (CVD) and fibre curvature (FC) differences between nine sampling sites vary between sex and flock, to identify differences in variability between sampling sites as a result of between animal and between sire variability and to determine correlations between sampling sites in between animal and between sire ...
carcinogenesis; elderly; protein synthesis; neoplasms; dog diseases; immunohistochemistry; dogs; skin diseases; gene expression; base pair mismatch; DNA repair; mast cells; disease resistance; animal genetics
Abstract:
... Cutaneous mast cell tumors (MCTs) are common canine neoplasms. Some dog breeds more frequently develop MCTs, suggesting a genetically mediated predisposition. In humans, the most common inherited cancer predisposition is caused by germline defects in the mismatch repair (MMR) genes. To investigate whether inherited defects in the MMR genes predispose some dogs to MCT development, MMR expression in ...
... The objective of this experiment was to determine whether the caspase proteolytic system has a role in postmortem tenderization. Six ewes and 6 wethers that were noncarriers and 6 ewes and 6 wethers that were expressing the callipyge gene were used for this study. Caspase activities were determined in LM at 7 different time points during the postmortem storage period: 0 h, 4 h, 8 h, 24 h, 2 d, 7 d ...
... Four genomic regions located on goat chromosomes 1, 2, 5 and 13 previously reported as significant DNA regions harboring quantitative trait loci (QTL) for growth and fleece traits in goat were considered as candidate genomic regions. The objective of conducting this study was to determine whether there is evidence for association of growth traits and cashmere yield in native Iranian Rayini goats w ...
animal genetics; dogs; canine hip dysplasia; hips; genes; genomics; disease incidence; breed differences; dog breeds; single nucleotide polymorphism; body conformation; prediction; breeding value
Abstract:
... Hip dysplasia is a common inherited trait of dogs that results in secondary osteoarthritis. In this article the methods used to uncover the mutations contributing to this condition are reviewed, beginning with hip phenotyping. Coarse, genome-wide, microsatellite-based screens of pedigrees of greyhounds and dysplastic Labrador retrievers were used to identify linked quantitative trait loci (QTL). F ...
... The avian inner perivitelline layer (IPVL) contains zona pellucida protein-B1 (ZPB1), zona pellucida protein-C (ZPC) and zona pellucida protein-D (ZPD). These three proteins may be involved in sperm binding to the IPVL. ZPB1 is produced by the liver and transported to the developing preovulatory follicle, while ZPC and ZPD are synthesized and secreted by the granulosa cells of the preovulatory fol ...
cattle; animal genetics; bacterial artificial chromosomes; chromosome mapping; DNA libraries; DNA fingerprinting
Abstract:
... Background: In large genomics projects involving many different types of analyses of bacterial artificial chromosomes (BACs), such as fingerprinting, end sequencing (BES) and full BAC sequencing there are many opportunities for the identities of BACs to become confused. However, by comparing the results from the different analyses, inconsistencies can be identified and a set of high integrity BACs ...
wildlife; ecology; population genetics; genetic techniques and protocols; genomics; animal genetics
Abstract:
... Noninvasive genetic approaches continue to improve studies in molecular ecology, conservation genetics and related disciplines such as forensics and epidemiology. Noninvasive sampling allows genetic studies without disturbing or even seeing the target individuals. Although noninvasive genetic sampling has been used for wildlife studies since the 1990s, technological advances continue to make nonin ...
... Scrotal hernia in pigs is a complex trait likely affected by genetic and environmental factors. A large-scale association analysis of positional and functional candidate genes was conducted in four previously identified genomic regions linked to hernia susceptibility on Sus scrofa chromosomes 2 and 12, as well as the fifth region around 67 cM on chromosome 2, respectively. In total, 151 out of 416 ...
... Research has shown that variation in ovine immunoglobulin A (IgA) levels are associated with reduced faecal egg counts (FECs) in sheep hosting gastro-intestinal (GI) parasites. Variation in the constant region of the ovine IgA heavy alpha chain gene (IGHA) may result in structurally and functionally different IgA molecules and may consequently lead to variation in the IgA response to parasitisatio ...
aquarium fish; animal genetics; DNA; genetic techniques and protocols; DNA libraries; ornamental fish
Abstract:
... Background: Trade in ornamental fishes represents, by far, the largest route for the importation of exotic vertebrates. There is growing pressure to regulate this trade with the goal of ensuring that species are sustainably harvested and that their point of origin is accurately reported. One important element of such regulation involves easy access to specimen identifications, a task that is curre ...
... An experimental Hereford herd established in 1960 was used from 1986 to 2006 to select for increased weaning weight (W) without increasing birth weight (B). Data were B and W collected over the 47 yr from 2,124 calves. Including ancestors, the pedigree file had 2,369 animals. Selection was practiced only in males. In the first stage (1986 to 1993), mass-selected bulls were chosen with the index I ...
... Pork odour is to a great extent affected by the presence of malodorous compounds, mainly androstenone and skatole. The present review outlines the current state of knowledge about factors involved in the regulation of androstenone and skatole in entire male pigs. Androstenone is a pheromonal steroid synthesised in the testes and metabolised in the liver. Part of androstenone accumulates in adipose ...
... The aims of this study were to estimate phenotypic and genetic parameters for a range of traits in Hungarian Sport Horses, and to compare several methods of estimating breeding value (BV) in this breed. The analyses were based on the Hungarian Sport Horse Studbook, results of self-performance tests (SPTs) and show-jumping competition results. An SPT comprises subjective judgement of conformation t ...
... Interspersed repeat composition and distribution in mammals have been best characterized in the human and mouse genomes. The bovine genome contains typical eutherian mammal repeats, but also has a significant number of long interspersed nuclear element RTE (BovB) elements proposed to have been horizontally transferred from squamata. Our analysis of the BovB repeats has indicated that only a few of ...
... Hybridisation-capture was used to create 12 unique alpaca DNA libraries each enriched for a different tetranucleotide microsatellite motif. Two hundred and forty-nine microsatellites were found, of which 26 were polymorphic (motifs GGAT, GTTT and GCAC). Nine markers were fully characterised on 45 samples. Allele numbers ranged from 6 (locus P135) to 12 (loci P149 and PCTD17). There was no evidence ...
... Using bacteria artificial chromosome (BAC) end sequences (16.9 Mb) and high-quality alignments of genomic sequences (17.4 Mb), we performed a global assessment of the divergence distributions, phylogenies, and consensus sequences for Alu elements in primates including lemur, marmoset, macaque, baboon, and chimpanzee as compared to human. We found that in lemurs, Alu elements show a broader and mor ...
... Variance components were estimated for maturing age, first litter size, and animal size in Finnish minks. The fitted animal models had direct genetic and maternal genetic effects, litter effects, and maternal environmental effects. Multivariate analysis was performed to determine covariances between the traits. Maternal effects represented a significant source of phenotypic variance in the maturat ...
... Oligonucleotide arrays are increasingly used in comparative genomic hybridization (CGH) to detect genomic copy number variation (CNV). The design of these arrays usually prefers uniquely mapped probes but routinely includes multiply mapped probes within a genome to maintain the high coverage and resolution. These duplicated probes could cause several limitations: besides their effects on the CNV c ...