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You searched for: Publication Year 2007 Remove constraint Publication Year: 2007 Subject Cryptococcus neoformans Remove constraint Subject: Cryptococcus neoformans
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1. An Anti-β-Glucan Monoclonal Antibody Inhibits Growth and Capsule Formation of Cryptococcus neoformans In Vitro and Exerts Therapeutic, Anticryptococcal Activity In Vivo

Author:
Rachini, Anna; Pietrella, Donatella; Lupo, Patrizia; Torosantucci, Antonella; Chiani, Paola; Bromuro, Carla; Proietti, Carla; Bistoni, Francesco; Cassone, Antonio; Vecchiarelli, Anna
Source:
Infection and immunity 2007 v.75 no.11 pp. 5085-5094
ISSN:
0019-9567
Subject:
Candida albicans; Cryptococcus albidus; Cryptococcus neoformans; antifungal properties; antigen-antibody reactions; beta-glucans; cell walls; encapsulation; fungi; host-pathogen relationships; humans; immunoglobulins; macrophages; mice; monoclonal antibodies; monocytes; phagocytosis; phospholipases; protective effect; proteinases
Abstract:
... In this study we tested the in vitro and in vivo anti-Cryptococcus neoformans activity of an antilaminarin (anti-β-glucan) monoclonal antibody (MAb 2G8) (immunoglobulin G2b) which was previously shown to inhibit the growth of β-glucan-exposing Candida albicans cells. Here we show that MAb 2G8 binds to the cell wall of C. neoformans and inhibits its growth to an extent comparable to that observed f ...
DOI:
10.1128/IAI.00278-07
PubMed:
17606600
PubMed Central:
PMC2168274

2. Anticandidial and anticryptococcal activity of Euphorbia fusiformis, a rare medicinal plant

Author:
Natarajan, D.; Nagamurugan, N.; Ramachandran, A.; Mohanasundari, C.; Srinivasan, K.
Source:
World journal of microbiology & biotechnology 2007 v.23 no.5 pp. 719-721
ISSN:
0959-3993
Subject:
Candida albicans; Cryptococcus neoformans; Euphorbia; antifungal properties; candidiasis; cryptococcosis; fungi; humans; leaves; medicinal plants; rootstocks; screening; solvents
Abstract:
... The present work demonstrates the screening of extracts of the rare medicinal herb Euphorbia fusiformis for antifungal activity. The main aim was to investigate its antifungal properties against Candida albicans and Cryptococcus neoformans, the causative agents of human candidiasis and cryptococcosis, respectively. Aqueous and organic solvent extracts from the leaves and rootstock of the plant wer ...
DOI:
10.1007/s11274-006-9275-5

3. Antileishmanial and antifungal activity of plants used in traditional medicine in Brazil

Author:
Braga, F.G.; Bouzada, M.L.M.; Fabri, R.L.; Matos, M. de O.; Moreira, F.O.; Scio, E.; Coimbra, E.S.
Source:
Journal of ethnopharmacology 2007 v.111 no.2 pp. 396-402
ISSN:
0378-8741
Subject:
Vernonia; Ocimum gratissimum; Schinus; Cajanus cajan; Piper aduncum; Bixa orellana; Syzygium cumini; medicinal plants; chemical constituents of plants; plant extracts; medicinal properties; Leishmania amazonensis; Leishmania donovani; antiprotozoal agents; Candida albicans; Cryptococcus neoformans; antifungal agents; traditional medicine; herbal medicines; ethnobotany; Brazil
Abstract:
... The antileishmanial and antifungal activity of 24 methanol extracts from 20 plants, all of them used in the Brazilian traditional medicine for the treatment of several infectious and inflammatory disorders, were evaluated against promastigotes forms of two species of Leishmania (L. amazonensis and L. chagasi) and two yeasts (Candida albicans and Cryptococcus neoformans). Among the 20 tested methan ...
DOI:
10.1016/j.jep.2006.12.006

4. Antimicrobial activity and bioactive compounds of Portuguese wild edible mushrooms methanolic extracts

Author:
Barros, Lillian; Calhelha, Ricardo C.; Vaz, Josiana A.; Ferreira, Isabel C. F. R.; Baptista, Paula; Estevinho, Letícia M.
Source:
European food research & technology 2007 v.225 no.2 pp. 151-156
ISSN:
1438-2377
Subject:
Bacillus cereus; Candida albicans; Cryptococcus neoformans; Escherichia coli; Gram-negative bacteria; Gram-positive bacteria; Lactarius deliciosus; Sarcodon; Tricholoma; antimicrobial properties; flavonoids; minimum inhibitory concentration; mushrooms; pathogens; phenols
Abstract:
... The antimicrobial properties of phenolic extracts of Portuguese wild edible mushroom species (Lactarius deliciosus, Sarcodon imbricatus and Tricholoma portentosum) against pathogens were investigated. The minimal inhibitory concentrations (MICs) were evaluated for the entire mushroom, the cap and the stipe, separately; the portion of the mushroom used proved to be influenced in the results obtaine ...
DOI:
10.1007/s00217-006-0394-x

5. Antimicrobial activity of limonene enantiomers and 1,8-cineole alone and in combination

Author:
Vuuren, S.F. van; Viljoen, A.M.
Source:
Flavour and fragrance journal 2007 v.22 no.6 pp. 540-544
ISSN:
0882-5734
Subject:
Cryptococcus neoformans; Pseudomonas aeruginosa; Staphylococcus aureus; anti-infective properties; cineole; enantiomers; essential oils; minimum inhibitory concentration; pathogens; yeasts
Abstract:
... Two common essential oil constituents, 1,8-cineole and limonene, were assessed for antimicrobial activity, using the minimum inhibitory concentration (MIC) microtitre plate method against eight organisms. The limonene enantiomers, i.e. (+), (-) and the racemate, singularly and in combination (1:1) with 1,8-cineole, were investigated to establish possible interactions. The MIC values were in the ra ...
DOI:
10.1002/ffj.1843

6. Binding of Serum Mannan Binding Lectin to a Cell Integrity-Defective Cryptococcus neoformans ccr4Δ Mutant

Author:
Panepinto, John C.; Komperda, Kazimierz W.; Hacham, Moshe; Shin, Soowan; Liu, Xiaoguang; Williamson, Peter R.
Source:
Infection and immunity 2007 v.75 no.10 pp. 4769-4779
ISSN:
0019-9567
Subject:
Cryptococcus neoformans; animal models; blood serum; cell walls; encapsulation; fungi; lectins; mutants; mycoses; virulence; virulent strains
Abstract:
... Mannan binding lectin (MBL) is an innate immune mediator belonging to the collectin family known to bind to the surfaces of many viruses, bacteria, and fungi. However, pathogenic strains of the fungus Cryptococcus neoformans are resistant to MBL binding. To dissect the mechanism of cryptococcal resistance to MBL, we compared MBL binding to an encapsulated wild-type strain, an encapsulated ccr4Δ mu ...
DOI:
10.1128/IAI.00536-07
PubMed:
17646356
PubMed Central:
PMC2044520

7. Biotinylation and characterization of Cryptococcus neoformans cell surface proteins

Author:
Foster, A.J.; Bird, R.A.; Smith, S.N.
Source:
Journal of applied microbiology 2007 v.103 no.2 pp. 390-399
ISSN:
1364-5072
Subject:
Cryptococcus neoformans; affinity chromatography; biotin; biotinylation; cell division; cell walls; confocal microscopy; encapsulation; flow cytometry; fluorescein; fluorescence; polyacrylamide gel electrophoresis; surface proteins
Abstract:
... To develop a novel procedure for isolating and characterizing cryptococcal cell-surface proteins using biotinylation, fluorescein isothiocyanate (FITC)-streptavidin, flow cytometry and associated ligand-receptor analysis, confocal microscopy and electrophoretic separation. Cell proteins of both acapsulate and encapsulated Cryptococcus neoformans cells were labelled using sulfo-NHS-biotin which, in ...
DOI:
10.1111/j.1365-2672.2006.03259.x
PubMed:
17650199

8. Cell Wall Targeting of Laccase of Cryptococcus neoformans during Infection of Mice

Author:
Waterman, Scott R.; Hacham, Moshe; Panepinto, John; Hu, Guowu; Shin, Soowan; Williamson, Peter R.
Source:
Infection and immunity 2007 v.75 no.2 pp. 714-722
ISSN:
0019-9567
Subject:
Cryptococcus neoformans; albino; brain; cell walls; fungi; green fluorescent protein; immunocompromised population; laccase; lungs; macrophages; mice; pH; virulence
Abstract:
... Laccase is a major virulence factor of the pathogenic fungus Cryptococcus neoformans, which afflicts both immunocompetent and immunocompromised individuals. In the present study, laccase was expressed in C. neoformans lac1Δ cells as a fusion protein with an N-terminal green fluorescent protein (GFP) using C. neoformans codon usage. The fusion protein was robustly localized to the cell wall at phys ...
DOI:
10.1128/IAI.01351-06
PubMed:
17101662
PubMed Central:
PMC1828480

9. Characterization of Environmental Sources of the Human and Animal Pathogen Cryptococcus gattii in British Columbia, Canada, and the Pacific Northwest of the United States

Author:
Kidd, Sarah E.; Chow, Yat; Mak, Sunny; Bach, Paxton J.; Chen, Huiming; Hingston, Adrian O.; Kronstad, James W.; Bartlett, Karen H.
Source:
Applied and environmental microbiology 2007 v.73 no.5 pp. 1433-1443
ISSN:
0099-2240
Subject:
Cryptococcus neoformans; acid soils; air; animal pathogens; carbon; central nervous system; freshwater; geographical variation; humans; hybrids; islands; niches; seawater; serotypes; soil pH; summer; trees; wild animals; British Columbia; Northwestern United States; Pacific States
Abstract:
... Cryptococcus gattii has recently emerged as a primary pathogen of humans and wild and domesticated animals in British Columbia, particularly on Vancouver Island. C. gattii infections are typically infections of the pulmonary and/or the central nervous system, and the incidence of infection in British Columbia is currently the highest reported globally. Prior to this emergence, the environmental di ...
DOI:
10.1128/AEM.01330-06
PubMed:
17194837
PubMed Central:
PMC1828779

10. Characterization of glycoinositolphosphoryl ceramide structure mutant strains of Cryptococcus neoformans

Author:
Gutierrez, Ana LS; Farage, Layla; Melo, Manuel N.; Mohana-Borges, Ronaldo S.; Guerardel, Yann; Coddeville, Bernadete; Wieruszeski, Jean-Michel; Mendonça-Previato, Lucia; Previato, Jose O.
Source:
Glycobiology 2007 v.17 no.6 pp. 1C
ISSN:
0959-6658
Subject:
Cryptococcus neoformans; alcohol oxidoreductases; biochemical pathways; biosynthesis; carboxy-lyases; ceramides; fungi; moieties; mutants; nuclear magnetic resonance spectroscopy; tandem mass spectrometry; viability; virulence
Abstract:
... In fungi, glycoinositolphosphoryl ceramide (GIPC) biosynthetic pathway produces essential molecules for growth, viability, and virulence. In previous studies, we demonstrated that the opportunistic fungus Cryptococcus neoformans synthesizes a complex family of xylose-(Xyl) branched GIPCs, all of which have not been previously reported in fungi. As an effort to understand the biosynthesis of these ...
DOI:
10.1093/glycob/cwm030

11. Cryptococcal phospholipase B antigen is not detected in serum of patients infected with Cryptococcus neoformans using a sandwich enzyme-linked immunosorbent assay

Author:
Wu, Qi Xuan; Chen, Sharon C.A.; Santangelo, Rosemary T.; Martin, Patricia; Malik, Richard; Sorrell, Tania C.
Source:
FEMS yeast research 2007 v.7 no.3 pp. 465-470
ISSN:
1567-1356
Subject:
Cryptococcus gattii; Cryptococcus neoformans; antigen detection; antigens; blood serum; cryptococcosis; detection limit; enzyme-linked immunosorbent assay; lysophospholipase; patients; rats; serodiagnosis; virulence; yeasts
Abstract:
... Extracellular phospholipase B (PLB) is a virulence determinant of Cryptococcus neoformans and Cryptococcus gattii. In this study, we developed a sensitive enzyme-linked immunosorbent assay (ELISA) for PLB antigen with a detection limit of 3.9 ng mL⁻¹. PLB was detected in culture supernatants of C. neoformans and C. gattii. PLB, however, was not detected in sera of seven human patients and 10 felin ...
DOI:
10.1111/j.1567-1364.2006.00187.x

12. Cryptococcus neoformans Can Utilize the Bacterial Melanin Precursor Homogentisic Acid for Fungal Melanogenesis

Author:
Frases, Susana; Salazar, Angela; Dadachova, Ekaterina; Casadevall, Arturo
Source:
Applied and environmental microbiology 2007 v.73 no.2 pp. 615-621
ISSN:
0099-2240
Subject:
Cryptococcus neoformans; acid tolerance; acids; alcohols; bacteria; bleaching; electron paramagnetic resonance spectroscopy; free radicals; fungi; glucose; hydrophobicity; irradiation; laccase; mammals; melanin; melanization; serotypes; solvents; ultraviolet radiation; virulence; wavelengths
Abstract:
... Cryptococcus neoformans melanizes in the environment and in mammalian tissues, but the process of melanization in either venue is mysterious given that this microbe produces melanin only from exogenous substrates. Understanding the process of melanization is important because melanization is believed to protect against various stresses in the environment, including UV radiation, and pigment produc ...
DOI:
10.1128/AEM.01947-06
PubMed:
17098915
PubMed Central:
PMC1796974

13. Cryptotrichosporon anacardii gen. nov., sp. nov., a new trichosporonoid capsulate basidiomycetous yeast from Nigeria that is able to form melanin on niger seed agar

Author:
Okoli, Ikechukwu; Oyeka, Christie A.; Kwon-Chung, Kyung J.; Theelen, Bart; Robert, Vincent; Groenewald, Johannes Z.; McFadden, Diane C.; Casadevall, Arturo; Boekhout, Teun
Source:
FEMS yeast research 2007 v.7 no.2 pp. 339-350
ISSN:
1567-1356
Subject:
Cryptococcus neoformans; agar; cashew nuts; flowers; genes; melanin; new species; nucleotide sequences; ribosomal RNA; trees; yeasts; Nigeria
Abstract:
... Five yeast isolates obtained from cashew tree flowers in Nigeria resembled Cryptococcus neoformans phenotypically by producing brown pigmented colonies on niger seed agar, expressing a capsule, and being able to grow at 37°C. However, rRNA gene sequences, including the 18S rRNA gene, the D1/D2 domains of the 26S rRNA gene and the ITS1+2 regions, suggested that these yeasts form a basal lineage wit ...
DOI:
10.1111/j.1567-1364.2006.00164.x

14. Cytoskeletal structures, ultrastructural characteristics and the capsule of the basidiomycetous yeast Cryptococcus laurentii

Author:
David, Marek; Gabriel, Miroslav; Kopecká, Marie
Source:
Antonie van Leeuwenhoek 2007 v.92 no.1 pp. 29-36
ISSN:
0003-6072
Subject:
Cryptococcus laurentii; Cryptococcus neoformans; actin; buds; cell walls; microtubules; mitosis; mitotic spindle apparatus; pathogens; ultrastructure; yeasts
Abstract:
... The cytoskeleton, capsule and cell ultrastructure were studied during the cell cycle of Cryptococcus laurentii. In an encapsulated strain, cytoplasmic microtubules and a mitotic spindle were detected. Mitosis was preceded by migration of the nucleus into the bud. F-actin failed to be visualised by rhodamine-phalloidin (RhPh) in encapsulated cells and therefore an acapsular strain was used. The fol ...
DOI:
10.1007/s10482-006-9131-5
PubMed:
17136567

15. Dependence of Macrophage Phagocytic Efficacy on Antibody Concentration

Author:
Macura, Nataša; Zhang, Tong; Casadevall, Arturo
Source:
Infection and immunity 2007 v.75 no.4 pp. 1904-1915
ISSN:
0019-9567
Subject:
Cryptococcus neoformans; antigens; complement; fungi; ingestion; macrophages; mathematical models; opsonins; phagocytosis; receptors
Abstract:
... Macrophages ingest the fungus Cryptococcus neoformans only in the presence of opsonins, and this provides a remarkably clean system for the detailed analysis of phagocytosis. This system is also unusual in that antibody-mediated phagocytosis involves ingestion through both Fc and complement receptors in the absence of complement. Mathematical modeling was used to analyze and explain the experiment ...
DOI:
10.1128/IAI.01258-06
PubMed:
17283107
PubMed Central:
PMC1865677

16. Depletion of Alveolar Macrophages Decreases the Dissemination of a Glucosylceramide-Deficient Mutant of Cryptococcus neoformans in Immunodeficient Mice

Author:
Kechichian, Talar B.; Shea, John; Del Poeta, Maurizio
Source:
Infection and immunity 2007 v.75 no.10 pp. 4792-4798
ISSN:
0019-9567
Subject:
phenotype; immunosuppression; niches; brain; macrophages; granuloma; mice; animal models; Cryptococcus neoformans; mutants; lungs; virulence
Abstract:
... In previous studies we showed that a Cryptococcus neoformans mutant lacking glucosylceramide (Δgcs1) is avirulent and unable to reach the brain when it is administered intranasally into an immunocompetent mouse and is contained in a lung granuloma. To determine whether granuloma formation is key for containment of C. neoformans Δgcs1, we studied the role of C. neoformans glucosylceramide in a T- a ...
DOI:
10.1128/IAI.00587-07
PubMed:
17664261
PubMed Central:
PMC2044542

17. Eca1, a Sarcoplasmic/Endoplasmic Reticulum Ca²⁺-ATPase, Is Involved in Stress Tolerance and Virulence in Cryptococcus neoformans

Author:
Fan, Weihua; Idnurm, Alexander; Breger, Julia; Mylonakis, Eleftherios; Heitman, Joseph
Source:
Infection and immunity 2007 v.75 no.7 pp. 3394-3405
ISSN:
0019-9567
Subject:
Acanthamoeba castellanii; Amoeba; Ca2-transporting ATPase; Caenorhabditis elegans; Cryptococcus neoformans; Galleria mellonella; body temperature; endoplasmic reticulum; fungi; genes; insect larvae; macrophages; mice; models; moths; mutants; osmotic stress; pathogens; phenotype; protein subunits; soil; stress tolerance; virulence
Abstract:
... The basidiomycetous fungal pathogen Cryptococcus neoformans is adapted to survive challenges in the soil and environment and within the unique setting of the mammalian host. A C. neoformans mutant was isolated with enhanced virulence in a soil amoeba model that nevertheless exhibits dramatically reduced growth at mammalian body temperature (37°C). This mutant phenotype results from an insertion in ...
DOI:
10.1128/IAI.01977-06
PubMed:
17502401
PubMed Central:
PMC1932933

18. Exploration of whole-genome responses of the human AIDS-associated yeast pathogen Cryptococcus neoformans var grubii: nitric oxide stress and body temperature

Author:
Chow, Eric D.; Liu, Oliver W.; O'Brien, Sean; Madhani, Hiten D.
Source:
Current genetics 2007 v.52 no.3-4 pp. 137-148
ISSN:
0172-8083
Subject:
Cryptococcus neoformans; Escherichia coli; antioxidants; bacteria; body temperature; cerebrospinal fluid; genes; human diseases; humans; mannitol; microarray technology; nitric oxide; oligonucleotides; pathogens; patients; transcription (genetics); virulence; yeasts
Abstract:
... Cryptococcus neoformans var grubii is an opportunistic basidiomycete yeast pathogen that is a significant cause of HIV/AIDS-related deaths worldwide. We describe a whole-genome oligonucleotide microarray for this pathogen. These arrays have been used to elucidate the transcriptional responses of the genome to heat shock as well as to two conditions relevant to human infections: body temperature an ...
DOI:
10.1007/s00294-007-0147-9

19. Extracellular glycosylphosphatidylinositol-anchored mannoproteins and proteases of Cryptococcus neoformans

Author:
Eigenheer, Richard A.; Jin Lee, Young; Blumwald, Eduardo; Phinney, Brett S.; Gelli, Angie
Source:
FEMS yeast research 2007 v.7 no.4 pp. 499-510
ISSN:
1567-1356
Subject:
Aspergillus fumigatus; Cryptococcus neoformans; animal pathogenic fungi; antigens; aspartic proteinases; beta-glucanase; cell walls; cryptococcosis; dialysis; high performance liquid chromatography; mass spectrometry; metalloproteinases; pathogenesis; peptides; proteolysis; proteome; proteomics; signal peptide; solid phase extraction; trypsin; vaccine development; yeasts
Abstract:
... Extracellular proteins of Cryptococcus neoformans are involved in the pathogenesis of cryptococcosis, and some are immunoreactive antigens that may potentially serve as candidates for vaccine development. To further study the extracellular proteome of the human fungal pathogen Cry. neoformans, we conducted a proteomic analysis of secreted and cell wall-bound proteins with an acapsular strain of Cr ...
DOI:
10.1111/j.1567-1364.2006.00198.x

20. Human Immunoglobulin G2 (IgG2) and IgG4, but Not IgG1 or IgG3, Protect Mice against Cryptococcus neoformans Infection

Author:
Beenhouwer, David O.; Yoo, Esther M.; Lai, Chun-Wei; Rocha, Miguel A.; Morrison, Sherie L.
Source:
Infection and immunity 2007 v.75 no.3 pp. 1424-1435
ISSN:
0019-9567
Subject:
Cryptococcus neoformans; blood serum; clinical trials; complement; correlation; death; half life; humans; immunoglobulin G; infectious diseases; meningitis; mice; monoclonal antibodies; patients; receptors; therapeutics; yeasts
Abstract:
... The encapsulated yeast Cryptococcus neoformans is a significant cause of meningitis and death in patients with AIDS. Some murine monoclonal antibodies (MAbs) against the glucuronoxylomannan (GXM) component of the C. neoformans capsular polysaccharide can prolong the lives of infected mice, while others have no effect or can even shorten survival. To date, no one has systematically compared the eff ...
DOI:
10.1128/IAI.01161-06
PubMed:
17220317
PubMed Central:
PMC1828574

21. Inhibition of chitin synthase 2 and antifungal activity of lignans from the stem bark of Lindera erythrocarpa

Author:
Hwang, E.I.; Leek Y.M.; Lee, S.M.; Yeo, W.H.; Moon, J.S.; Kang, T.H.; Park, K.D.; Kim, S.U.
Source:
Planta medica 2007 v.73 no.7 pp. 679-682
ISSN:
0032-0943
Subject:
Lindera; Colletotrichum orbiculare; Candida albicans; Saccharomyces cerevisiae; lignans; chitin synthase; Cryptococcus neoformans; Aspergillus fumigatus; enzyme inhibition; antifungal properties; South Korea
DOI:
10.1055/s-2007-981526
PubMed:
17538872

22. Microbial Metalloproteinases Mediate Sensing of Invading Pathogens and Activate Innate Immune Responses in the Lepidopteran Model Host Galleria mellonella

Author:
Altincicek, Boran; Linder, Monica; Linder, Dietmar; Preissner, Klaus T.; Vilcinskas, Andreas
Source:
Infection and immunity 2007 v.75 no.1 pp. 175-183
ISSN:
0019-9567
Subject:
Cryptococcus neoformans; Galleria mellonella; elicitors; genes; hemolymph; immune response; immune system; insects; larvae; lipopolysaccharides; lysozyme; melanization; metalloproteinases; models; pathogens; prophenoloxidase; proteins; proteolysis; proteomics; reverse transcriptase polymerase chain reaction; serine proteinases; transcription (genetics); virulence
Abstract:
... Thermolysin-like metalloproteinases such as aureolysin, pseudolysin, and bacillolysin represent virulence factors of diverse bacterial pathogens. Recently, we discovered that injection of thermolysin into larvae of the greater wax moth, Galleria mellonella, mediated strong immune responses. Thermolysin-mediated proteolysis of hemolymph proteins yielded a variety of small-sized (<3 kDa) protein fra ...
DOI:
10.1128/IAI.01385-06
PubMed:
17074843
PubMed Central:
PMC1828416

23. Modulation of the Pulmonary Type 2 T-Cell Response to Cryptococcus neoformans by Intratracheal Delivery of a Tumor Necrosis Factor Alpha-Expressing Adenoviral Vector

Author:
Milam, Jami E.; Herring-Palmer, Amy C.; Pandrangi, Raj; McDonald, Roderick A.; Huffnagle, Gary B.; Toews, Galen B.
Source:
Infection and immunity 2007 v.75 no.10 pp. 4951-4958
ISSN:
0019-9567
Subject:
nitric oxide synthase; transgenes; interferon-gamma; interleukin-5; macrophages; chitinase; major histocompatibility complex; mice; mycoses; tumor necrosis factor-alpha; Cryptococcus neoformans; lungs; interleukin-4; eosinophilia; Human betaherpesvirus 5; fungi
Abstract:
... C57BL/6 mice develop an allergic bronchopulmonary mycosis following intratracheal inoculation of Cryptococcus neoformans 24067. We determined that only low levels of tumor necrosis factor alpha (TNF-α) are produced in the lungs following infection. Thus, the objective of the present studies was to determine whether treatment with a TNF-α-expressing adenoviral vector (adenoviral vector with the mur ...
DOI:
10.1128/IAI.00176-07
PubMed:
17646355
PubMed Central:
PMC2044519

24. Molecular typing and antifungal susceptibility of clinical sequential isolates of Cryptococcus neoformans from Sao Paulo State, Brazil

Author:
Almeida, Ana Marisa Fusco; Matsumoto, Marcelo Teruyuki; Baeza, Lilian Cristiane; Oliveira e Silva, Rosana Bellan de; Kleiner, Aline Aparecida Pizzirani; Melhem, Márcia de Souza Carvalho; Mendes Giannini, Maria José Soares
Source:
FEMS yeast research 2007 v.7 no.1 pp. 152-164
ISSN:
1567-1356
Subject:
Cryptococcus gattii; Cryptococcus neoformans; DNA; amphotericin B; electrophoresis; fluconazole; flucytosine; genetic variation; itraconazole; karyotyping; meningitis; minimum inhibitory concentration; mixed infection; patients; random amplified polymorphic DNA technique; yeasts; Brazil
Abstract:
... The antifungal susceptibility profiles and the genetic variability of 83 sequential clinical isolates of Cryptococcus neoformans, including four Cryptococcus gattii isolates, obtained from 38 Sao Paulo AIDS patients with cryptococcal meningitis were assessed by electrophoretic karyotyping and random amplified polymorphic DNA (RAPD) analysis. The majority of the Cryptococcus neoformans isolates wer ...
DOI:
10.1111/j.1567-1364.2006.00128.x

25. Protection against Cryptococcosis by Using a Murine Gamma Interferon-Producing Cryptococcus neoformans Strain

Author:
Wormley, Floyd L. Jr.; Perfect, John R.; Steele, Chad; Cox, Gary M.
Source:
Infection and immunity 2007 v.75 no.3 pp. 1453-1462
ISSN:
0019-9567
Subject:
humans; interferon-gamma; mice; methodology; Cryptococcus neoformans; immune response; vaccine development; chemokines; fungi
Abstract:
... We evaluated cell-mediated immune (CMI) responses in mice given a pulmonary infection with a Cryptococcus neoformans strain engineered to produce the Th1-type cytokine gamma interferon (IFN-γ). Mice given a pulmonary infection with an IFN-γ-producing C. neoformans strain were able to resolve the primary infection and demonstrated complete (100%) protection against a second pulmonary challenge with ...
DOI:
10.1128/IAI.00274-06
PubMed:
17210668
PubMed Central:
PMC1828544

26. Pulmonary cryptococcosis in slaughtered sheep: Anatomopathology and culture

Author:
Lemos, L.S.; Santos, A.S. de O. dos; Vieira-da-Motta, O.; Texeira, G.N.; Carvalho, E.C.Q. de
Source:
Veterinary microbiology 2007 v.125 no.3-4 pp. 350-354
ISSN:
0378-1135
Subject:
sheep; sheep diseases; respiratory tract diseases; mycoses; Cryptococcus neoformans; disease detection; case studies; epidemiological studies; pathogen identification; lungs; tissue analysis; cryopreservation; histopathology; yeasts; epithelium; viability; microscopy; differential staining; in vitro culture; risk assessment; disease transmission; zoonoses; Brazil
Abstract:
... Cryptococcosis is considered a zoonosis but is rare in stock animals. In humans it is imputed to be a mortality cause in immunocompromised patients. This case study reports the anatomopathology of respiratory cryptococcosis in ovine and the recuperation and identification of Cryptococcus neoformans var. neoformans from 1-year frozen lung tissue sample. The findings from haematoxylin/eosin staining ...
DOI:
10.1016/j.vetmic.2006.11.028

27. The Relative Susceptibility of Mouse Strains to Pulmonary Cryptococcus neoformans Infection Is Associated with Pleiotropic Differences in the Immune Response

Author:
Zaragoza, Oscar; Alvarez, Mauricio; Telzak, Andrew; Rivera, Johanna; Casadevall, Arturo
Source:
Infection and immunity 2007 v.75 no.6 pp. 2729-2739
ISSN:
0019-9567
Subject:
Cryptococcus neoformans; T-lymphocytes; blood serum; brain; immune response; immunoglobulin E; immunoglobulin G; immunoglobulin M; interleukin-10; interleukin-12; intravenous injection; macrophages; mice; models; pathogenesis; survival rate
Abstract:
... CBA/J mice were highly susceptible to intratracheal (i.t.) Cryptococcus neoformans infection relative to BALB/c mice, while both strains were equally susceptible to intravenous (i.v.) infection. Increased susceptibility in i.t. infection was associated with higher brain CFU, lower serum immunoglobulin M (IgM) and IgG responses to glucuronoxylomannan (GXM), lack of IgE regulation during infection, ...
DOI:
10.1128/IAI.00094-07
PubMed:
17371865
PubMed Central:
PMC1932903

28. Sequence Analysis and Expression of a Blue-Light Photoreceptor Gene, Le.phrA from the Basidiomycetous Mushroom Lentinula edodes

Author:
Hiroaki SANO; Takatsugu NARIKIYO; Shinya KANEKO; Takashi YAMAZAKI; Kazuo SHISHIDO
Source:
Bioscience, biotechnology, and biochemistry 2007 v.71 no.9 pp. 2206-2213
ISSN:
1347-6947
Subject:
Coprinopsis cinerea; Cryptococcus neoformans; Lentinula edodes; Neurospora crassa; biotechnology; blue light; genes; mushrooms; mycelium; photoreceptors; sequence analysis
Abstract:
... We cloned and sequenced a photoreceptor gene (Le.phrA) from the basidiomycete Lentinula edodes. The product of Le.phrA, Le.PHRA (924 aa residues) contained a serine-rich region, an LOV domain and two PAS domains. It was clearly smaller than other fungal LOV domain-containing blue-light photoreceptors such as Coprinopsis cinerea Dst1 (1,175 aa), Neurospora crassa WC-1 (1,167 aa), and Cryptococcus n ...
DOI:
10.1271/bbb.70170

29. The mating type-specific homeodomain genes SXI1α and SXI2a coordinately control uniparental mitochondrial inheritance in Cryptococcus neoformans

Author:
Yan, Zhun; Hull, Christina M.; Sun, Sheng; Heitman, Joseph; Xu, Jianping
Source:
Current genetics 2007 v.51 no.3 pp. 187-195
ISSN:
0172-8083
Subject:
Cryptococcus neoformans; crossing; cytoplasmic inheritance; eukaryotic cells; genes; mitochondria; mitochondrial DNA; mitochondrial genome; parents; progeny; transposons; yeasts; zygote
Abstract:
... In the great majority of sexual eukaryotes, mitochondrial genomes are inherited almost exclusively from a single parent. While many hypotheses have been proposed to explain this phenomenon, very little is known about the genetic elements controlling uniparental mitochondria inheritance. In the bipolar, isogamous basidiomycete yeast Cryptococcus neoformans, progeny from crosses between strains of m ...
DOI:
10.1007/s00294-006-0115-9