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You searched for: Publication Year 2007 Remove constraint Publication Year: 2007 Subject DNA-directed RNA polymerase Remove constraint Subject: DNA-directed RNA polymerase Subject gene expression Remove constraint Subject: gene expression Subject DNA microarrays Remove constraint Subject: DNA microarrays
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1. Disruption of essential plastid gene expression caused by T7 RNA polymerase-mediated transcription of plastid transgenes during early seedling development

Author:
Magee, Alan M.; MacLean, Daniel; Gray, John C.; Kavanagh, Tony A.
Source:
Transgenic research 2007 v.16 no.4 pp. 415-428
ISSN:
0962-8819
Subject:
DNA microarrays; DNA-directed RNA polymerase; bacteriophages; biotechnology; gels; gene expression; gene expression regulation; genetically modified organisms; leaves; mutants; phenotype; proteins; ribosomal RNA; seedlings; tobacco; transcription (genetics); transgenes
Abstract:
... Transcription of plastid transgenes by plastid-targeted T7 RNA polymerase (ptT7RNAP) during early seedling development in tobacco was associated with a pale-green leaf phenotype, depletion of plastid rRNAs and arrest of shoot development. Extensive analysis of mutant seedlings at the transcript level using DNA microarrays and RNA gel blotting revealed severe disruption of plastid rRNA accumulation ...
DOI:
10.1007/s11248-006-9045-z
PubMed:
17103239

2. The role of the omega subunit of RNA polymerase in expression of the relA gene in Escherichia coli

Author:
Chatterji, Dipankar; Ogawa, Yoshito; Shimada, Tomohiro; Ishihama, Akira
Source:
FEMS microbiology letters 2007 v.267 no.1 pp. 51-55
ISSN:
0378-1097
Subject:
messenger RNA; starvation; stress response; DNA microarrays; gene expression; ribosomal proteins; operon; DNA-directed RNA polymerase; Escherichia coli
Abstract:
... The rpoZ gene for the omega subunit of Escherichia coli RNA polymerase constitutes single operon with the spoT gene, which is responsible for the maintenance of stringent response under nutrient starvation conditions. To identify the physiological role of the omega subunit, we compared the gene expression profile of wild-type Escherichia coli with that of an rpoZ deleted strain by microarray analy ...
DOI:
10.1111/j.1574-6968.2006.00532.x