U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

You searched for: Publication Year 2007 Remove constraint Publication Year: 2007 Subject term cryopreservation Remove constraint Subject term: cryopreservation Text availability Citation in PubAg Remove constraint Text availability: Citation in PubAg
Start Over

Search

160  Search Results

« Previous | 1 - 50 of 160 |
Number of results to display per page

Search Results

1. Antagonist effect of DMSO on the cryoprotection ability of glycerol during cryopreservation of buffalo sperm

Author:
Rasul, Z.; Ahmed, N.; Anzar, M.
Source:
Theriogenology 2007 v.68 no.5 pp. 813-819
ISSN:
0093-691X
Subject:
buffaloes; bulls; semen; cryopreservation; cryoprotectants; glycerol; dimethyl sulfoxide; temperature; semen extenders; spermatozoa; sperm motility
Abstract:
... The objective of the present study was to investigate the synergistic effect of DMSO and glycerol added at various temperatures on the post-thaw quality of buffalo sperm. Pooled ejaculates from four Nili-Ravi buffalo bulls were divided into 18 aliquots and extended (1:10) in Tris-citric acid extender differing in glycerol:DMSO ratios (0:0, 0:1.5, 0:3; 3:0, 3:1.5, 3:3; and 6:0, 6:1.5, 6:3, respecti ...
DOI:
10.1016/j.theriogenology.2007.06.014

2. Association between seasonal changes in fatty-acid composition, expression of VLDL receptor and bovine sperm quality

Author:
Argov, N.; Sklan, D.; Zeron, Y.; Roth, Z.
Source:
Theriogenology 2007 v.67 no.4 pp. 878-885
ISSN:
0093-691X
Subject:
lipid content; messenger RNA; bulls; cryopreservation; fatty acid composition; very low density lipoprotein; sperm motility; cholesterol; seminal plasma; seasonal variation; spermatozoa; receptors
Abstract:
... Reduced reproductive performance of dairy cows during the summer is often associated with elevated temperature. Semen collected and cryopreserved during the summer may be of low quality and might contribute to the compromised fertility of dairy cows during this season. The present study examined the association between seasonality, semen quality and its potential to survive cryopreservation. A com ...
DOI:
10.1016/j.theriogenology.2006.10.018

3. Biometry of frozen-thawed sperm from eight breeds of Indian buffaloes (Bubalus bubalis)

Author:
Aggarwal, R.A.K.; Ahlawat, S.P.S.; Kumar, Y.; Panwar, P.S.; Singh, K.; Bhargava, M.
Source:
Theriogenology 2007 v.68 no.4 pp. 682-686
ISSN:
0093-691X
Subject:
breed differences; buffaloes; bulls; morphometry; cryopreservation; male fertility; spermatozoa
Abstract:
... Sperm morphometry, in combination with other objective traits, can be useful for developing a fertility index. The objective of the present study was to measure various biometric end points of frozen-thawed sperm from eight breeds of Indian buffaloes (Murrah, Surti, Tarai, Mehsana, Jaffrabadi, Bhadawari, Pandharpuri and Nili-Ravi). The sperm head of Pandharpuri buffaloes had the greatest length (1 ...
DOI:
10.1016/j.theriogenology.2007.03.028

4. Comparison of Different Extenders with Defined Protein Composition for Storage of Stallion Spermatozoa at 5°C

Author:
Aurich, C.; Seeber, P.; Müller-Schlösser, F.
Source:
Reproduction in domestic animals 2007 v.42 no.4 pp. 445-448
ISSN:
0936-6768
Subject:
caseinates; cold storage; cryopreservation; dried skim milk; male fertility; protein composition; protein sources; semen; semen extenders; soy protein; spermatozoa; stallions; whey protein
Abstract:
... To maintain the fertility of stallion spermatozoa during cooled storage, extender media are added to semen. In this study, three semen extenders were compared: EquiPro which contains defined caseinates and whey proteins instead of dried skim milk. The extender is provided in dry form and dissolved in distilled water prior to use. EquiPro TM has the same composition as EquiPro but is provided in a ...
DOI:
10.1111/j.1439-0531.2006.00828.x

5. Cryogelation phenomena in mixed skim milk powder -- barley β-glucan-polyol aqueous dispersions

Author:
Lazaridou, A.; Biliaderis, C.G.
Source:
Food research international 2007 v.40 no.7 pp. 793-802
ISSN:
0963-9969
Subject:
thermal stability; dispersions; sorbitol; barley; aqueous solutions; glucose; xylose; fructose; molecular weight; microstructure; sucrose; electron microscopy; dried skim milk; beta-glucans; blended foods; deformation; mixing; cryopreservation; freeze-thaw cycles; gelatinization
Abstract:
... The effects of polyols (fructose, glucose, sorbitol, sucrose and xylose) on cryostructurization of barley mixed-linkage (1 leads to 3), (1 leads to 4) β-D-glucans (β-glucans) in the presence of skim milk proteins were investigated; three β-glucan isolates differing in molecular size (apparent molecular weight of 210, 140 and 70 x 10(3) Da) were used. Polyols were incorporated at 15% (w/w) concentr ...
DOI:
10.1016/j.foodres.2007.01.016

6. Cryopreservation and orthotopic transplantation of rat ovaries as a means of gamete banking

Author:
Martina M Dorsch; Dirk Wedekind; Kenji Kamino; Hans J Hedrich
Source:
Laboratory animals 2007 v.41 no.2 pp. 247-254
ISSN:
1758-1117
Subject:
rats; surgical transplantation; cryopreservation
Abstract:
... Besides the exponentially increasing number of mouse strains, the rising number of rat strains, due to the establishment of transgenic and coisogenic strains in this species, surpasses the capacity of most animal houses. Cryopreservation of gametes may be a means of solving these problems. Here we describe an easy and fast method for the cryopreservation and transplantation of frozen–thawed ovarie ...
DOI:
10.1258/002367707780378195
PubMed:
17430624

7. Cryopreservation of European Mouflon (Ovis Gmelini Musimon) Semen During the non-Breeding Season is Enhanced by the Use of Trehalose

Author:
Berlinguer, F.; Leoni, GG; Succu, S.; Mossa, F.; Galioto, M.; Madeddu, M.; Naitana, S.
Source:
Reproduction in domestic animals 2007 v.42 no.2 pp. 202-207
ISSN:
0936-6768
Subject:
Ovis; Pisum sativum; acrosome; agglutinins; cryopreservation; iodides; mouflon; oocytes; rams; semen; thawing; trehalose; viability
Abstract:
... The influence of trehalose on European mouflon spermatozoa cryopreservation during the non-breeding season was tested. Semen was frozen in two different extenders: (a) recommended Tris-based ram extender (CTR); (b) CTR extender supplemented with trehalose 0.147 m m (TRH). Sperm viability and acrosome integrity were assessed using propidium iodide and fluorescein isothiocynate labelled Pisum Sativu ...
DOI:
10.1111/j.1439-0531.2006.00753.x
PubMed:
17348979

8. Cryopreservation of Primordial Germ Cells (PGCs) from White Leghorn Embryos Using Commercial Cryoprotectants

Author:
Setioko, Argono R.; Tagami, Takahiro; Tase, Hideki; Nakamura, Yoshiaki; Takeda, Kumiko; Nirasawa, Keijiro
ISSN:
1346-7395
Subject:
chickens; viability; germ cells; laboratory techniques; cryopreservation; embryo (animal); cryoprotectants; White Leghorn; blood cells; thawing
Abstract:
... Cryopreservation of poultry semen has been reported, but preservation of female genetic material has not been possible because of the large size of their yolk structure. Cryopreservation of primordial germ cells (PGCs) is an alternative way to preserve both female and male genetic material in chicken. In the present study, fertilized eggs were incubated for about 53 hours to obtain embryos at stag ...

9. Cryopreservation of carnation (Dianthus caryophyllus L.) shoot tips by encapsulation-vitrification

Author:
Halmagyi, A.; Deliu, C.
Source:
Scientia horticulturae 2007 v.113 no.3 pp. 300-306
ISSN:
0304-4238
Subject:
floriculture crops; cryopreservation; plant growth substances; explants; artificial seeds; encapsulation; Dianthus caryophyllus; micropropagation; shoot meristems; culture media
DOI:
10.1016/j.scienta.2007.04.002

10. Cryopreservation of immature and in vitro matured porcine oocytes by solid surface vitrification

Author:
Gupta, M.K.; Uhm, S.J.; Lee, H.T.
Source:
Theriogenology 2007 v.67 no.2 pp. 238-248
ISSN:
0093-691X
Subject:
cytochalasin B; viability; cell cleavage; in vitro culture; cryopreservation; dimethyl sulfoxide; cryoprotectants; ethylene glycol; pretreatment; in vitro fertilization; oocytes; swine
Abstract:
... Cryopreservation of normal, lipid-containing porcine oocytes has had limited practical success. This study used solid surface vitrification (SSV) of immature germinal vesicle (GV) and mature meiosis II (MII) porcine oocytes and evaluated the effects of pretreatment with cytochalasin B, cryoprotectant type (dimethylsulfoxide (DMSO), ethylene glycol (EG), or both), and warming method (two-step versu ...
DOI:
10.1016/j.theriogenology.2006.07.015

11. Cryopreservation of in vitro matured buffalo (Bubalus bubalis) oocytes by minimum volumes vitrification methods

Author:
Gasparrini, B.; Attanasio, L.; De Rosa, A.; Monaco, E.; Di Palo, R.; Campanile, G.
Source:
Animal reproduction science 2007 v.98 no.3-4 pp. 335-342
ISSN:
0378-4320
Subject:
protective effect; solutions; viability; buffaloes; cryopreservation; culture media; cryoprotectants; methodology; blastocyst; Bubalus; yields; embryogenesis; germplasm conservation; oocytes; adverse effects; thawing; in vitro fertilization
DOI:
10.1016/j.anireprosci.2006.04.046

12. Cryopreservation of sperm of the endangered Gila trout, Oncorhynchus gilae

Author:
Fuller, S.A.
Source:
Journal of applied aquaculture 2007 v.19 no.1 pp. 71-79
ISSN:
1045-4438
Subject:
fish culture; Oncorhynchus; trout; endangered species; spermatozoa; cryopreservation; cryoprotectants; germplasm conservation; semen extenders
DOI:
10.1300/J028v19n01_07

13. Cryopreservation of sperm of two European percid species, the pikeperch (Sander lucioperca) and the Volga pikeperch (S. volgensis)

Author:
Z. Bokor; T. Müller; M. Bercsényi; L. Horváth; B. Urbányi; Á. Horváth
Source:
Acta biologica Hungarica 2007 v.58 no.2 pp. 199-207
ISSN:
0236-5383
Subject:
Sander lucioperca; Sander volgensis; cryopreservation; cryoprotectants; dimethyl sulfoxide; fertilizer rates; fish; glucose; hatching; methanol; potassium chloride; spermatozoa; sucrose; urine
Abstract:
... Experiments were carried out on sperm cryopreservation of two European percid fish species, the pikeperch (Sander lucioperca) and the Volga pikeperch (S. volgensis) . Two experiments were conducted on pikeperch sperm. In the first, the effects of three extenders (Glucose, KCl, Sucrose) and two cryoprotectants (dimethyl-sulfoxide: DMSO, methanol: MeOH) were tested on motility and fert ...
DOI:
10.1556/ABiol.58.2007.2.6

14. Cryopreservation of tench, Tinca tinca, sperm: Sperm motility and hatching success of embryos

Author:
Rodina, M.; Gela, D.; Kocour, M.; Alavi, S.M.H.; Hulak, M.; Linhart, O.
Source:
Theriogenology 2007 v.67 no.5 pp. 931-940
ISSN:
0093-691X
Subject:
fertilization (reproduction); Tinca tinca; spermatozoa; cryopreservation; cryoprotectants; dimethyl sulfoxide; propanediols; storage time; sperm motility; fish eggs; embryo (animal); hatching; fish larvae
Abstract:
... The aim of the present study was to elaborate cryopreservation methods for ex situ conservation of tench. Success of cryopreservation was tested during two series of experiments. The first set of experiments studied the effects of two types of cryoprotectants (DMSO and a combination of DMSO with propanediol at ratio 1:1) at concentrations of 8 and 10% and three different equilibration times in two ...
DOI:
10.1016/j.theriogenology.2006.11.007

15. Cryopreservation of transgenic rice suspension cells producing recombinant hCTLA4Ig

Author:
Ji-Suk Cho; Seok-Mi Hong; Sung-Yeon Joo; Ji-Seon Yoo; Dong-Il Kim
Source:
Applied microbiology and biotechnology 2007 v.73 no.6 pp. 1470-1476
ISSN:
0175-7598
Subject:
Oryza sativa; T-lymphocytes; antigens; callus; cell viability; cooling; cryopreservation; cryoprotectants; cytotoxicity; dimethyl sulfoxide; freezers; glycerol; humans; mannitol; nitrogen; rice; sodium chloride; sorbitol; storage; sucrose; transgenic plants; trehalose
Abstract:
... Transgenic suspension cells of Oryza sativa L. cv. Dongjin utilized as a host for producing recombinant human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) were preserved in liquid nitrogen (−196 °C) after slow prefreezing in a deep freezer (−70 °C). The development of an optimal procedure for long-term storage was investigated by the addition of various concentrations of cryoprotecta ...
DOI:
10.1007/s00253-006-0627-8
PubMed:
17021872

16. Cryoprotectant optimization for sperm of diploid Pacific oysters by use of commercial dairy sperm freezing facilities

Author:
Dong, Q.; Huang, C.; Eudeline, B.; Tiersch, T.R.
Source:
Aquaculture 2007 v.271 no.1-4 pp. 537-545
ISSN:
0044-8486
Subject:
Crassostrea gigas; oysters; spermatozoa; cryopreservation; cryoprotectants; dimethyl sulfoxide; ethylene glycol; methanol; propylene; freezing; oyster culture
Abstract:
... Although sperm cryopreservation has been practiced in aquatic species for more than 50 years, viable markets for frozen sperm do not currently exist for commercial aquaculture. The present study suggests that the use of commercial cryopreservation facilities used for dairy bulls could be a cost-effective approach to initiate commercialization of frozen sperm in aquaculture, and the oyster industry ...
DOI:
10.1016/j.aquaculture.2007.07.005

17. Cryosurvival of bovine blastocysts is enhanced by culture with trans-10 cis-12 conjugated linoleic acid (10t,12c CLA)

Author:
Pereira, R.M.; Baptista, M.C.; Vasques, M.I.; Horta, A.E.M.; Portugal, P.V.; Bessa, R.J.B.; Silva, J.C.; Pereira, M.S.; Marques, C.C.
Source:
Animal reproduction science 2007 v.98 no.3-4 pp. 293-301
ISSN:
0378-4320
Subject:
protective effect; viability; oocysts; lipid content; microscopy; bioaccumulation; cryopreservation; cattle; embryogenesis; isomers; embryo (animal); bovine serum albumin; conjugated linoleic acid; culture media; in vitro fertilization
DOI:
10.1016/j.anireprosci.2006.03.015

18. Decrease in glutathione (GSH) content in bovine sperm after cryopreservation: Comparison between two extenders

Author:
Stradaioli, G.; Noro, T.; Sylla, L.; Monaci, M.
Source:
Theriogenology 2007 v.67 no.7 pp. 1249-1255
ISSN:
0093-691X
Subject:
chemical composition; semen extenders; freezing; glutathione; bulls; oxidative stress; cryopreservation; acrosome reaction; sperm motility; spermatozoa; thawing
Abstract:
... Growing evidence suggests that among the causes which deteriorate qualitative and functional characteristics of semen after freezing and thawing, there are those linked to oxidative stress and decrease detoxification of antioxidants present in the cells and seminal plasma. Overall, cryopreservation reduces the spermatozoal GSH content. In the present study we assessed the motility, capacitation st ...
DOI:
10.1016/j.theriogenology.2007.01.009

19. Detection of early changes in sperm membrane integrity pre-freezing can estimate post-thaw quality of boar spermatozoa

Author:
Peña, F.J.; Saravia, F.; Johannisson, A.; Wallgren, M.; Rodríguez-Martínez, H.
Source:
Animal reproduction science 2007 v.97 no.1-2 pp. 74-83
ISSN:
0378-4320
Subject:
boars; animal breeding; artificial insemination; cryopreservation; male fertility; screening; cell membranes; freezing quality; viability; differential staining; histopathology; tissue analysis; microstructure; microscopy; spermatozoa
DOI:
10.1016/j.anireprosci.2005.12.014

20. Development and quality of sheep embryos cultured in commercial G1.3/G2.3 sequential media

Author:
Garcia-Garcia, R.M.; Ward, F.; Fair, S.; O'Meara, C.M.; Wade, M.; Duffy, P.; Lonergan, P.
Source:
Animal reproduction science 2007 v.98 no.3-4 pp. 233-240
ISSN:
0378-4320
Subject:
in vivo studies; viability; cryopreservation; sheep; embryo (animal); embryogenesis; culture media; adverse effects; thawing; in vitro fertilization
DOI:
10.1016/j.anireprosci.2006.03.007

21. Development of OPS vitrified pig blastocysts: Effects of size of the collected blastocysts, cryoprotectant concentration used for vitrification and number of blastocysts transferred

Author:
Berthelot, F.; Venturi, E.; Cognié, J.; Furstoss, V.; Martinat-Botté, F.
Source:
Theriogenology 2007 v.68 no.2 pp. 178-185
ISSN:
0093-691X
Subject:
swine; blastocyst; embryo culture; cryopreservation; cryoprotectants; chemical concentration; viability; embryonic mortality; embryo transfer; embryogenesis; pregnancy rate
Abstract:
... Unhatched blastocysts from Large White hyperprolific gilts (n = 103) were identified, measured and vitrified using the Open Pulled Straw (OPS) technique to evaluate the effects of the collected blastocyst size and cryoprotectant concentrations used for vitrification, and the number of embryos transferred per recipient. Vitrified/warmed blastocyst viability was estimated in vitro, as the percentage ...
DOI:
10.1016/j.theriogenology.2007.04.050

22. Development of a cryopreservation protocol for long-term storage of black tiger shrimp (Penaeus monodon) spermatophores

Author:
Vuthiphandchai, V.; Nimrat, S.; Kotcharat, S.; Bart, A.N.
Source:
Theriogenology 2007 v.68 no.8 pp. 1192-1199
ISSN:
0093-691X
Subject:
fertilization (reproduction); Penaeus monodon; shrimp; spermatophores; cryopreservation; cryoprotectants; chemical concentration; frozen storage; storage temperature; storage time; freezing; thawing; spermatozoa; viability; egg hatchability
Abstract:
... The objectives of this study were to determine the effect of cryoprotectants on sperm viability and develop a freezing protocol for long-term storage of P. monodon spermatophores. Spermatophores suspended for 30 min in calcium-free saline (Ca-F saline) containing the cryoprotectants dimethyl sulfoxide (DMSO), ethylene glycol (EG), 1,2-propylene glycol (PG), formamide, and methanol at concentration ...
DOI:
10.1016/j.theriogenology.2007.08.024
PubMed:
17900683

23. Developmental competence of equine oocytes and embryos obtained by in vitro procedures ranging from in vitro maturation and ICSI to embryo culture, cryopreservation and somatic cell nuclear transfer

Author:
Galli, C.; Colleoni, S.; Duchi, R.; Lagutina, I.; Lazzari, G.
Source:
Animal reproduction science 2007 v.98 no.1-2 pp. 39-55
ISSN:
0378-4320
Subject:
cryopreservation; animal reproduction; embryo (animal); biotechnology; somatic cells; embryo culture; nuclear transplantation; cytoplasm; embryogenesis; oocytes; horses; intracytoplasmic sperm injection
DOI:
10.1016/j.anireprosci.2006.10.011

24. Dialysis of boar semen prior to freezing-thawing: Its effects on post-thaw sperm characteristics

Author:
Fraser, L.; Dziekońska, A.; Strzeżek, R.; Strzeżek, J.
Source:
Theriogenology 2007 v.67 no.5 pp. 994-1003
ISSN:
0093-691X
Subject:
semen extenders; boars; cryopreservation; sperm motility; adenosine triphosphate; mitochondria; acrosome; seminal plasma; plasma membrane; dialysis
Abstract:
... Low-molecular weight components of the seminal plasma have a detrimental effect on sperm function. The present study was undertaken to evaluate the effect of the removal of low-molecular weight components by dialysis on sperm characteristics prior to and after freezing. Semen, collected from 5 boars, was extended in Kortowo-3 extender (K-3, Poland) and cooled for 3 h (control non-dialysis) or dial ...
DOI:
10.1016/j.theriogenology.2006.12.002

25. Directional Freezing of Equine Semen in Large Volumes

Author:
Saragusty, J.; Gacitua, H.; Pettit, MT; Arav, A.
Source:
Reproduction in domestic animals 2007 v.42 no.6 pp. 610-615
ISSN:
0936-6768
Subject:
cryopreservation; freezing; industry; nitrogen; pregnancy rate; semen; spermatozoa; stallions; thawing; viability
Abstract:
... Despite its potential impact on the horse industry, sperm cryopreservation is not an established technology throughout the industry, for a number of reasons that include a reduction in pregnancy rate and increased cost per pregnancy. We have evaluated a novel directional freezing technique, based on a multi-thermal gradient (MTG), by comparing it with the conventional, controlled-rate cryopreserva ...
DOI:
10.1111/j.1439-0531.2006.00831.x
PubMed:
17976068

26. Effect of Bradykinin on Murrah buffalo (Bubalus bubalis) semen cryopreservation

Author:
Shukla, M.K.; Misra, A.K.
Source:
Animal reproduction science 2007 v.97 no.1-2 pp. 175-179
ISSN:
0378-4320
Subject:
semen extenders; semen; viability; buffaloes; freezing quality; membrane permeability; cryopreservation; bradykinin; males; freeze-thaw cycles; sperm motility; osmoregulation; spermatozoa; artificial insemination; protective effect
DOI:
10.1016/j.anireprosci.2006.02.015

27. Effect of Insemination-Ovulation Interval and Addition of Seminal Plasma on Sow Fertility to Insemination of Cryopreserved Sperm

Author:
Abad, M.; Garcia, JC; Sprecher, DJ; Cassar, G.; Friendship, RM; Buhr, MM; Kirkwood, RN
Source:
Reproduction in domestic animals 2007 v.42 no.4 pp. 418-422
ISSN:
0936-6768
Subject:
insemination; equine chorionic gonadotropin; cryopreservation; sows; farrowing rate; freeze-thaw cycles; Yorkshire (swine breed); seminal plasma; spermatozoa; crossbreds; ovulation; artificial insemination; litter size
Abstract:
... In swine, the use of frozen-thawed (FT) sperm for artificial insemination (AI) is limited because of poor sow fertility, possibly associated with a post-thaw capacitation-like status resulting in fewer fully viable sperm. Sow fertility to AI with FT sperm may improve with deeper deposition of sperm within the female tract, insemination very close to ovulation, or reversal of cryocapacitation by se ...
DOI:
10.1111/j.1439-0531.2006.00801.x
PubMed:
17635780

28. Effect of Sperm Cryopreservation on the European Eel Sperm Viability and Spermatozoa Morphology

Author:
Asturiano, JF; Marco-Jiménez, F.; Peñaranda, DS; Garzón, DL; Pérez, L.; Vicente, JS; Jover, M.
Source:
Reproduction in domestic animals 2007 v.42 no.2 pp. 162-166
ISSN:
0936-6768
Subject:
Anguilla anguilla; cell viability; cryopreservation; developmental stages; dimethyl sulfoxide; proteins; sperm motility; spermatozoa; thawing
Abstract:
... The main objective of the present work was to study the effect of cryopreservation of European eel sperm both on the sperm viability and the spermatozoa head morphology. Spermatozoa morphology was evaluated with computer-assisted morphology analysis after collection in fresh samples, after adding the freezing medium containing dimethyl sulfoxide as cryoprotectant and, finally, after the cryopreser ...
DOI:
10.1111/j.1439-0531.2006.00746.x
PubMed:
17348973

29. Effect of antioxidant supplementation on semen quality and reactive oxygen species of frozen-thawed canine spermatozoa

Author:
Michael, A.; Alexopoulos, C.; Pontiki, E.; Hadjipavlou-Litina, D.; Saratsis, P.; Boscos, C.
Source:
Theriogenology 2007 v.68 no.2 pp. 204-212
ISSN:
0093-691X
Subject:
semen extenders; semen; hydroxyl radicals; cryopreservation; dogs; sperm motility; acrosome; superoxide anion; additives
Abstract:
... The objective of this study was to evaluate post-thaw quality of frozen dog semen processed with diluents containing different antioxidants. Ejaculates were collected, pooled and evaluated for concentration, motility, rapid steady forward movement (RSF movement), viability, acrosomal integrity and by the hypo-osmotic swelling test. Also, superoxide production, hydroxyl radicals and total reactive ...
DOI:
10.1016/j.theriogenology.2007.04.053

30. Effect of cryopreservation on viability, activation and growth of in situ and isolated ovine early-stage follicles

Author:
Santos, R.R.; Hurk, R. van den; Rodrigues, A.P.R.; Costa, S.H.F.; Martins, F.S.; Matos, M.H.T.; Celestino, J.J.H.; Figueiredo, J.R.
Source:
Animal reproduction science 2007 v.99 no.1-2 pp. 53-64
ISSN:
0378-4320
Subject:
viability; follicular development; freezing; cryopreservation; ovarian follicles; dimethyl sulfoxide; differential staining; cryoprotectants; ethylene glycol; ewes; dyes; thawing; animal breeding; germplasm conservation; culture media; adverse effects; animal tissues; tissue culture
DOI:
10.1016/j.anireprosci.2006.05.013

31. Effect of exogenous calcium on post-thaw growth recovery and subsequent plant regeneration of cryopreserved embryogenic calli of Hevea brasiliensis (Müll. Arg.)

Author:
Lardet, Ludovic; Martin, Florence; Dessailly, Florence; Carron, Marc-Philippe; Montoro, Pascal
Source:
Plant cell reports 2007 v.26 no.5 pp. 559-569
ISSN:
0721-7714
Subject:
Hevea brasiliensis; calcium; calcium chloride; callus; cryopreservation
Abstract:
... A reliable cryopreservation technique was developed for friable embryogenic callus lines of Hevea brasiliensis. The study showed that reducing the CaCl₂ concentration of the pre-culture medium from 9 mM to 1 or 0 mM CaCl₂ before cryopreservation promoted post-thaw callus growth, 1 mM being the optimum CaCl₂ concentration for embryo regeneration. Post-thaw callus proliferation decreased in line wit ...
DOI:
10.1007/s00299-006-0278-3
PubMed:
17186244

32. Effect of lactose and glycerol on the motility, normal apical ridge, chromatin condensation and chromatin stability of frozen boar spermatozoa

Author:
Corcuera, B.D.; Marigorta, P.; Sagüés, A.; Saiz-Cidoncha, F.; Pérez-Gutiérrez, J.F.
Source:
Theriogenology 2007 v.67 no.6 pp. 1150-1157
ISSN:
0093-691X
Subject:
chemical concentration; semen extenders; semen; lactose; boars; cryopreservation; glycerol; sperm motility; cryoprotectants; acrosome; chromatin
Abstract:
... The effect of lactose and glycerol concentration, as well as the equilibration time with glycerol was studied on motility, normal apical ridge (NAR), and chromatin state of boar spermatozoa after the freezing and thawing process. In the first experiment, samples were frozen in first and second extenders containing different concentrations of lactose (11, 12 and 14%). In the second experiment sampl ...
DOI:
10.1016/j.theriogenology.2007.01.002

33. Effect of low density lipoprotein on DNA integrity of freezing-thawing boar sperm by neutral comet assay

Author:
Jiang, Z.L.; Li, Q.W.; Li, W.Y.; Hu, J.H.; Zhao, H.W.; Zhang, S.S.
Source:
Animal reproduction science 2007 v.99 no.3-4 pp. 401-407
ISSN:
0378-4320
Subject:
protective effect; freezing; cold injury; DNA damage; boars; temporal variation; cryopreservation; assays; freeze-thaw cycles; low density lipoprotein; spermatozoa; animal breeding; germplasm conservation; additives
DOI:
10.1016/j.anireprosci.2006.08.022

34. Effect of macromolecules in solutions for vitrification of mature bovine oocytes

Author:
Checura, C.M.; Seidel, G.E. Jr.
Source:
Theriogenology 2007 v.67 no.5 pp. 919-930
ISSN:
0093-691X
Subject:
cattle; oocytes; cryopreservation; solutions; culture media; chemical composition; cryoprotectants; blood serum; in vitro fertilization; embryo culture; embryogenesis; blastocyst
Abstract:
... This study was designed to evaluate vitrification procedures for in vitro matured bovine oocytes for efficient blastocyst production after warming, IVF and culture. A second goal was to replace serum as the macromolecular component of the vitrification solution, without compromising efficacy. The first experiment compared two containers, open pulled straws (OPS) versus cryoloops, and two vitrifica ...
DOI:
10.1016/j.theriogenology.2006.09.044

35. Effect of the luteinizing hormone on embryo production in superovulated rabbit does

Author:
Salvetti, P.; Theau-Clément, M.; Beckers, J.F.; Hurtaud, J.; Guérin, P.; Neto, V.; Falières, J.; Joly, T.
Source:
Theriogenology 2007 v.67 no.6 pp. 1185-1193
ISSN:
0093-691X
Subject:
females; rabbits; superovulation; luteinizing hormone; follicle-stimulating hormone; corpus luteum; embryo (animal); viability; embryo culture; embryogenesis; cryopreservation
Abstract:
... For most domestic animals, the responses to superovulation treatments are not controlled as a consequence of the lack of knowledge on exogenous gonadotrophins effects on the ovarian function. The role of luteinizing hormone (LH) on the number and quality of embryos produced was evaluated on rabbit does superovulated with porcine FSH (pFSH). Parameters of embryos recovery, in vitro and in vivo embr ...
DOI:
10.1016/j.theriogenology.2007.01.013

36. Effect of vitrification solutions and cooling upon in vitro matured prepubertal ovine oocytes

Author:
Succi, S.; Leoni, G.G.; Berlinguer, F.; Madeddu, M.; Bebbere, D.; Mossa, F.; Bogliolo, l.; Ledda, S.; Naitana, S.
Source:
Theriogenology 2007 v.68 no.1 pp. 107-114
ISSN:
0093-691X
Subject:
cryopreservation; sheep; chromosome morphology; cryoprotectants; maturation-promoting factor; microtubules; chromatin; embryogenesis; oocytes; in vitro fertilization
Abstract:
... The vitrification procedure effects on molecular and cytoskeletal components and on developmental ability of in vitro matured prepubertal ovine oocytes were evaluated. MII oocytes were divided into three groups: (1) vitrified in cryoloops (VTR); (2) exposed to vitrification solutions and rehydrated without being plunged into liquid nitrogen (EXP); (3) without further treatment as a control (CTR). ...
DOI:
10.1016/j.theriogenology.2007.04.035

37. Effective use of encapsulation-dehydration technique in cryopreserving somatic embryos of butterfly pea (Clitoria ternatea L.)

Author:
Nair, D.S.; Reghunath, B.R.
Source:
Journal of herbs, spices & medicinal plants 2007 v.13 no.3 pp. 83-96
ISSN:
1049-6475
Subject:
Clitoria ternatea; ornamental plants; medicinal plants; nursery crops; specialty crops; cryopreservation; embryo (plant); somatic embryogenesis; plant genetic resources; germplasm; callus; culture media; micropropagation; 2,4-D; benzyladenine; germination; gibberellic acid; microencapsulation; mortality; viability; regrowth
DOI:
10.1300/J044v13n03_07

38. Effects of Cryopreservation on Bull Spermatozoa Distribution in Morphometrically Distinct Subpopulations

Author:
Rubio-Guillén, J.; González, D.; Garde, JJ; Esteso, MC; Fernández-Santos, MR; Rodríguez-Gíl, JE; Madrid-Bury, N.; Quintero-Moreno, A.
Source:
Reproduction in domestic animals 2007 v.42 no.4 pp. 354-357
ISSN:
0936-6768
Subject:
bulls; cryopreservation; freezing; glycerol; morphometry; nitrogen; semen; spermatozoa; thawing; vapors
Abstract:
... Assisted sperm morphometry analysis (ASMA) was used in this study to determine the effects of cryopreservation on bull spermatozoa distribution in morphometrically distinct subpopulations. Ejaculates were collected from five bulls and were divided. One portion was diluted at 30°C in a skim milk-egg yolk medium, containing glycerol. A microscope slide was prepared from single extended sperm samples ...
DOI:
10.1111/j.1439-0531.2006.00788.x
PubMed:
17635770

39. Effects of cryopreservation on sperm quality, nuclear DNA integrity, in vitro fertilization, and in vitro embryo development in the mouse

Author:
Yildiz, Cengiz; Ottaviani, Palma; Law, Napoleon; Ayearst, Renise; Liu, Ling; McKerlie, Colin
Source:
Reproduction 2007 v.133 no.3 pp. 585-595
ISSN:
1470-1626
Subject:
DNA; DNA fragmentation; animal models; blastocyst; chromatin; cryopreservation; cryoprotectants; embryogenesis; ethylene glycol; freezing; fructose; glucose; glycerol; human diseases; in vitro fertilization; mice; nuclear genome; plasma membrane; propylene glycol; protective effect; pyruvic acid; raffinose; research programs; skim milk; sodium; spermatozoa; thawing
Abstract:
... Efficient freezing, archiving, and thawing of sperm are essential techniques to support large scale research programs using mouse models of human disease. The purpose of this study was to investigate the effects of variable combinations and concentrations of cryoprotectants on sperm-assessment parameters of frozen-thawed mouse sperm in order to optimize cryopreservation protocols. Sperm was frozen ...
DOI:
10.1530/REP-06-0256
PubMed:
17379653

40. Effects of platelet-activating factor and platelet-activating factor: acetylhydrolase on in vitro post-thaw boar sperm parameters

Author:
Bathgate, R.; Maxwell, W.M.C.; Evans, G.
Source:
Theriogenology 2007 v.67 no.4 pp. 886-892
ISSN:
0093-691X
Subject:
semen extenders; semen; boars; cryopreservation; platelet-activating factor; sperm motility; cryoprotectants; hydrolases; acrosome; additives
Abstract:
... Cryopreservation of boar sperm compromises fertility after thawing by reducing sperm longevity and inducing acrosome reaction-like changes. In an attempt to improve the post-thaw motility and acrosome integrity of boar sperm, semen was frozen using a modified Westendorf method in which the medium was supplemented with either platelet-activating factor (PAF) or a recombinant platelet-activating fac ...
DOI:
10.1016/j.theriogenology.2006.09.043

41. Ejaculate and type of freezing extender affect rates of fertilization of horse oocytes in vitro

Author:
Roasa, L.M.; Choi, Y.H.; Love, C.C.; Romo, S.; Varner, D.D; Hinrichs, K.
Source:
Theriogenology 2007 v.68 no.4 pp. 560-566
ISSN:
0093-691X
Subject:
horses; ejaculation; semen; spermatozoa; cryopreservation; chemical composition; semen extenders; bovine serum albumin; storage time; oocytes; in vitro fertilization
Abstract:
... In vitro fertilization (IVF) was performed on in vitro-matured equine oocytes in three experiments. Frozen-thawed sperm were prepared using swim-up separation and heparin treatment. In Experiment 1, fertilization was achieved with sperm from only one frozen ejaculate of four obtained from the same stallion. Within this ejaculate, fertilization rates were higher with fresh media, as compared to med ...
DOI:
10.1016/j.theriogenology.2007.04.057

42. Experimental study on the freezing characteristics of four kinds of vegetables

Author:
Haiying, W.; Shaozhi, Z.; Guangming, C.
Source:
Lebensmittel-Wissenschaft + Technologie 2007 v.40 no.6 pp. 1112-1116
ISSN:
0023-6438
Subject:
mushrooms; cauliflower; navy beans; peas; pods; food preservation; freezing; pretreatment; glass transition temperature; freezing point; ice nucleation; differential scanning calorimetry; frozen vegetables; cryopreservation
DOI:
10.1016/j.lwt.2006.06.001

43. Extender osmolality and sugar supplementation exert a complex effect on the cryopreservation of Iberian red deer (Cervus elaphus hispanicus) epididymal spermatozoa

Author:
Fernández-Santos, M.R.; Martínez-Pastor, F.; García-Macías, V.; Esteso, M.C.; Soler, A.J.; Paz, P. de; Anel, L.; Garde, J.J.
Source:
Theriogenology 2007 v.67 no.4 pp. 738-753
ISSN:
0093-691X
Subject:
Cervus elaphus; deer; epididymis; spermatozoa; cryopreservation; semen extenders; osmotic pressure; additives; sugars; cryoprotectants; viability; sperm motility; mathematical models
Abstract:
... We have carried out two experiments to study the cryobiology of red deer epididymal spermatozoa and to improve freezing extenders: (1) effect of extender (Tris-citrate-fructose) osmolality (300-600 mOsm/kg), and (2) effect of sugar (0.4 M) supplementation to the extender (no sugar, glucose, fructose, mannose, sucrose, maltose, threalose and raffinose). Sperm quality was assessed pre-freezing, post ...
DOI:
10.1016/j.theriogenology.2006.10.005

44. Field performance characterization of strawberry (Fragaria x ananassa Duch.) plants derived from cryopreserved apices

Author:
Medina, J.J.; Clavero-Ramírez, I.; González-Benito, M.E.; Gálvez-Farfán, J.; López-Aranda, J.M.; Soria, C.
Source:
Scientia horticulturae 2007 v.113 no.1 pp. 28-32
ISSN:
0304-4238
Subject:
Fragaria ananassa; strawberries; cryopreservation; shoot meristems; apical meristems; in vitro regeneration; crop yield; field experimentation; agronomic traits; fruit quality; fruit set; micropropagation; phenotype; Spain
DOI:
10.1016/j.scienta.2007.01.030

45. Flow cytometry and ultrastructure of cryopreserved red seabream (Pagrus major) sperm

Author:
Liu, Q.H.; Li, J.; Zhang, S.C.; Xiao, Z.Z.; Ding, F.H.; Yu, D.D.; Xu, X.Z.
Source:
Theriogenology 2007 v.67 no.6 pp. 1168-1174
ISSN:
0093-691X
Subject:
fertilization (reproduction); Pagrus major; spermatozoa; cryopreservation; flow cytometry; ultrastructure; sperm motility; plasma membrane; mitochondria; hatching; bream; marine fish
Abstract:
... The objectives were to assess motility, fertilizing capacity, structural integrity, and mitochondrial function in fresh versus frozen-thawed (15% DMSO was used as a cryoprotectant) sperm from red seabream (Pagrus major). Mean (±S.D.) rates of motility, fertilization and hatching of frozen-thawed sperm were 81.0 ± 5.4, 92.8 ± 1.9, and 91.8 ± 5.2%, respectively; for fresh sperm, they were 87.5 ± 7.7 ...
DOI:
10.1016/j.theriogenology.2006.12.013

46. Gene expression and development of mouse zygotes following droplet vitrification

Author:
Dhali, A.; Anchamparuthy, V.M.; Butler, S.P.; Pearson, R.E.; Mullarky, I.K.; Gwazdauskas, F.C.
Source:
Theriogenology 2007 v.68 no.9 pp. 1292-1298
ISSN:
0093-691X
Subject:
cryopreservation; rapid methods; mice; zygote; blastocyst; gene expression; transcription (genetics); apoptosis; cell cleavage
Abstract:
... The concept of ultra-rapid vitrification has emerged in recent years; the accelerated cooling rate reduced injury attributed to cryopreservation and improved post-freezing developmental competence of vitrified oocytes and embryos. The objectives of the present study were to develop a simple and effective ultra-rapid vitrification method (droplet vitrification) and evaluate its effects on post-thaw ...
DOI:
10.1016/j.theriogenology.2007.08.030
PubMed:
17915304

47. Heparin and quercitin generate differential metabolic pathways that involve aminotransferases and LDH-X dehydrogenase in cryopreserved bovine spermatozoa

Author:
Cordoba, M.; Pintos, L.N.; Beconi, M.T.
Source:
Theriogenology 2007 v.67 no.3 pp. 648-654
ISSN:
0093-691X
Subject:
quercitrin; aspartate transaminase; bulls; cryopreservation; malate dehydrogenase; alanine transaminase; biochemical pathways; heparin; spermatozoa; enzyme activity; amino acid metabolism; lactate dehydrogenase
Abstract:
... Heparin and quercetin induce capacitation in spermatozoa through membrane receptor binding and inhibition of Ca-ATPase of the plasma membrane, respectively. Although capacitation is energy intensive, ammonia from amino acid metabolism can inhibit respiration and Krebs cycle activity. The objective was to determine activities of key enzymes in bull spermatozoa that contribute to the redox state and ...
DOI:
10.1016/j.theriogenology.2006.09.041
PubMed:
17084443

48. Highly efficient vitrification for cryopreservation of human oocytes and embryos: The Cryotop method

Author:
Kuwayama, M.
Source:
Theriogenology 2007 v.67 no.1 pp. 73-80
ISSN:
0093-691X
Subject:
human reproduction; humans; cryopreservation; laboratory techniques; biotechnology; blastocyst; assisted reproductive technologies; oocytes
Abstract:
... Vitrification is frequently referred to as a novel technology of cryopreservation in embryology, although some young embryologists were born after its first successful application. Unfortunately, in spite of the accumulated evidence regarding its enormous potential value, most domestic animal and human laboratories use exclusively the traditional slow-rate freezing with its compromised efficiency ...
DOI:
10.1016/j.theriogenology.2006.09.014

49. Immature bovine oocyte cryopreservation: Comparison of different associations with ethylene glycol, glycerol and dimethylsulfoxide

Author:
Yamada, C.; Caetano, H.V.A.; Simões, R.; Nicacio, A.C.; Feitosa, W.B.; Assumpção, M.E.O.D.; Visintin, J.A.
Source:
Animal reproduction science 2007 v.99 no.3-4 pp. 384-388
ISSN:
0378-4320
Subject:
cryopreservation; morphogenesis; dimethyl sulfoxide; glycerol; adverse effects; cryoprotectants; ethylene glycol; cows; toxicity; animal breeding; germplasm conservation; oocytes; chemical treatment
DOI:
10.1016/j.anireprosci.2006.07.001

50. Importation of in vitro-produced Bubalus bubalis embryos from Italy into the United States: A case report

Author:
BonDurant, R.H.; Drost, M.; Zambrano-Varon, J.; Campanile, G.; Gasparrini, B.; Zicarelli, L.
Source:
Theriogenology 2007 v.68 no.3 pp. 454-460
ISSN:
0093-691X
Subject:
buffaloes; embryo (animal); imports; embryo culture; cryopreservation; embryo transfer; import policies; trade regulations; Animal and Plant Health Inspection Service; Italy; United States
Abstract:
... On December 19, 2005, 14 in vitro-fertilized water buffalo (Bubalus bubalis) embryos, which had been cryopreserved by vitrification, were thawed and transferred into B. bubalis recipients in California. The embryos had been produced in Italy, following transvaginal oocyte pickup (TVOPU), with subsequent in vitro maturation, insemination, and culture. This case study relates our experience in meeti ...
DOI:
10.1016/j.theriogenology.2007.05.049
PubMed:
17582487