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You searched for: Publication Year 2007 Remove constraint Publication Year: 2007 Subject term cryopreservation Remove constraint Subject term: cryopreservation
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1. Adjustments on the cryopreservation conditions reduce the incidence of boar ejaculates with poor sperm freezability

Author:
Hernandez, M.; Roca, J.; Gil, M.A.; Vazquez, J.M.; Martinez, E.A.
Source:
Theriogenology 2007 v.67 no.9 pp. 1436-1445
ISSN:
0093-691X
Subject:
chemical concentration; freezing; semen; semen extenders; boars; ejaculation; cryopreservation; glycerol; sperm motility; spermatozoa; thawing
Abstract:
... The objective of the present study was to evaluate the effectiveness of different cryopreservation conditions (CCs) for freezing and thawing boar ejaculates, focusing on those having sub-optimal sperm freezability. Using a split-ejaculate technique, single ejaculates from 53 boars were diluted in lactose-egg yolk extender, containing a final glycerol concentration (GLY) of 2 or 3%, packaged in 0.5 ...
DOI:
10.1016/j.theriogenology.2007.02.012
PubMed:
17434207

2. Analysis of oocyte physiology to improve cryopreservation procedures

Author:
Gardner, D.K.; Sheehan, C.B.; Rienzi, L.; Katz-Jaffe, M.; Larman, M.G.
Source:
Theriogenology 2007 v.67 no.1 pp. 64-72
ISSN:
0093-691X
Subject:
membrane permeability; laboratory techniques; cryopreservation; calcium; cryoprotectants; mammals; oocytes
Abstract:
... In contrast to the preimplantation mammalian embryo, it has been notoriously difficult to cryopreserve the metaphase II oocyte. The ability to store oocytes successfully at -196 °C has numerous practical and financial advantages, together with ethical considerations, and will positively impact animal breeding programs and assisted conception in the human. Differences in membrane permeability and i ...
DOI:
10.1016/j.theriogenology.2006.09.012

3. Antagonist effect of DMSO on the cryoprotection ability of glycerol during cryopreservation of buffalo sperm

Author:
Rasul, Z.; Ahmed, N.; Anzar, M.
Source:
Theriogenology 2007 v.68 no.5 pp. 813-819
ISSN:
0093-691X
Subject:
buffaloes; bulls; semen; cryopreservation; cryoprotectants; glycerol; dimethyl sulfoxide; temperature; semen extenders; spermatozoa; sperm motility
Abstract:
... The objective of the present study was to investigate the synergistic effect of DMSO and glycerol added at various temperatures on the post-thaw quality of buffalo sperm. Pooled ejaculates from four Nili-Ravi buffalo bulls were divided into 18 aliquots and extended (1:10) in Tris-citric acid extender differing in glycerol:DMSO ratios (0:0, 0:1.5, 0:3; 3:0, 3:1.5, 3:3; and 6:0, 6:1.5, 6:3, respecti ...
DOI:
10.1016/j.theriogenology.2007.06.014

4. Antioxidant status and genotypic tolerance of Ribes in vitro cultures to cryopreservation

Author:
Johnston, J.W.; Harding, K.; Benson, E.E.
Source:
Plant science 2007 v.172 no.3 pp. 524-534
ISSN:
0168-9452
Subject:
cold tolerance; tissue culture; plant stress; free radicals; cryopreservation; genetic variation; Ribes nigrum; ethylene production; mortality; plant response; shoot meristems; currants; antioxidants; oxidative stress; phenolic compounds
DOI:
10.1016/j.plantsci.2006.11.001

5. Assessing genetic stability of a range of terrestrial microalgae after cryopreservation using amplified fragment length polymorphism (AFLP)

Author:
Müller, Julia; Day, John G.; Harding, Keith; Hepperle, Dominik; Lorenz, Maike; Friedl, Thomas
Source:
American journal of botany 2007 v.94 no.5 pp. 799-808
ISSN:
0002-9122
Subject:
DNA methylation; additives; amplified fragment length polymorphism; cryopreservation; free radicals; genetic stability; microalgae; mutants; mutation; phenotype; temperature
Abstract:
... Cryopreservation is the long-term, indefinite storage of living biological resources at ultralow temperatures. It is almost universally assumed that cryogenic storage supports genetic and phenotypic stability of organisms. However, certain components of the cryopreservation process, particularly some cryoprotective additives (CPAs) and free radical mediated cryoinjury, may potentially cause geneti ...
DOI:
10.3732/ajb.94.5.799
PubMed:
21636449

6. Assessment of Sperm Apoptosis in Cryopreserved Bull Semen After Swim-up Treatment: A Flow Cytometric Study

Author:
Chaveiro, A.; Santos, P.; da Silva, FM
Source:
Reproduction in domestic animals 2007 v.42 no.1 pp. 17-21
ISSN:
0936-6768
Subject:
adverse effects; apoptosis; bulls; cryopreservation; flow cytometry; in vitro fertilization; iodides; necrosis; plasma membrane; semen; spermatozoa
Abstract:
... The techniques used to prepare bovine spermatozoa for in vitro fertilization, to enhance the percentage of motile sperm cells include the swim-up (SU) method, among others. The objective of the present study was to evaluate the phosphatidylserine (PS) translocation and plasma membrane integrity as the indicator of apoptosis and necrosis in post-thaw bull sperm after SU treatment using annexin V-fl ...
DOI:
10.1111/j.1439-0531.2006.00712.x
PubMed:
17214767

7. Association between seasonal changes in fatty-acid composition, expression of VLDL receptor and bovine sperm quality

Author:
Argov, N.; Sklan, D.; Zeron, Y.; Roth, Z.
Source:
Theriogenology 2007 v.67 no.4 pp. 878-885
ISSN:
0093-691X
Subject:
lipid content; messenger RNA; bulls; cryopreservation; fatty acid composition; very low density lipoprotein; sperm motility; cholesterol; seminal plasma; seasonal variation; spermatozoa; receptors
Abstract:
... Reduced reproductive performance of dairy cows during the summer is often associated with elevated temperature. Semen collected and cryopreserved during the summer may be of low quality and might contribute to the compromised fertility of dairy cows during this season. The present study examined the association between seasonality, semen quality and its potential to survive cryopreservation. A com ...
DOI:
10.1016/j.theriogenology.2006.10.018

8. Batchwise assessment of porcine embryos for cryotolerance

Author:
Fujino, Y.; Kikuchi, K.; Nakamura, Y.; Kobayashi, H.; Yonemura, I.; Suzuki, M.; Misumi, K.; Nagai, T.
Source:
Theriogenology 2007 v.67 no.2 pp. 413-422
ISSN:
0093-691X
Subject:
viability; pregnancy rate; cryopreservation; farrowing rate; blastocyst; batch systems; embryogenesis; embryo culture; piglets; embryo transfer
Abstract:
... The viability or developmental ability of porcine embryos after slow-freezing and thawing differs depending on the embryonic stage or the batch, which is defined as a group of embryos obtained from one donor at one time. We froze porcine blastocysts in batches and assessed their cryotolerance by using two expanded blastocysts (EBs) as samples to predict the developmental potential of other blastoc ...
DOI:
10.1016/j.theriogenology.2006.08.008

9. Biometry of frozen-thawed sperm from eight breeds of Indian buffaloes (Bubalus bubalis)

Author:
Aggarwal, R.A.K.; Ahlawat, S.P.S.; Kumar, Y.; Panwar, P.S.; Singh, K.; Bhargava, M.
Source:
Theriogenology 2007 v.68 no.4 pp. 682-686
ISSN:
0093-691X
Subject:
breed differences; buffaloes; bulls; morphometry; cryopreservation; male fertility; spermatozoa
Abstract:
... Sperm morphometry, in combination with other objective traits, can be useful for developing a fertility index. The objective of the present study was to measure various biometric end points of frozen-thawed sperm from eight breeds of Indian buffaloes (Murrah, Surti, Tarai, Mehsana, Jaffrabadi, Bhadawari, Pandharpuri and Nili-Ravi). The sperm head of Pandharpuri buffaloes had the greatest length (1 ...
DOI:
10.1016/j.theriogenology.2007.03.028

10. Birth of offspring of pre-determined sex after artificial insemination of frozen-thawed, sex-sorted and re-frozen-thawed ram spermatozoa

Author:
De Graaf, S.P.; Evans, G.; Maxwell, W.M.C.; Cran, D.G.; O'Brien, J.K.
Source:
Theriogenology 2007 v.67 no.2 pp. 391-398
ISSN:
0093-691X
Subject:
sheep; artificial insemination; spermatozoa; cryopreservation; sex preselection; flow cytometry; male fertility; lambing rate
Abstract:
... The fertility of ram spermatozoa cryopreserved prior to, and following, sex-sorting by flow cytometry was assessed after insemination of mature Merino ewes at a synchronised estrus. Ewes were inseminated with spermatozoa from three rams, split into four treatment groups: 50 x 10⁶ motile non-sorted, frozen-thawed (Control50), 15 x 10⁶ motile non-sorted, frozen-thawed (Control15), 15 x 10⁶ motile se ...
DOI:
10.1016/j.theriogenology.2006.08.005

11. Birth of puppies after intrauterine and intratubal insemination with frozen-thawed canine semen

Author:
Kim, H.J.; Oh, H.J.; Jang, G.; Kim, M.K.
Source:
Journal of veterinary science 2007 v.8 no.1 pp. 75-80
ISSN:
1229-845X
Subject:
freezing; semen; pregnancy rate; animal fertility; whelping; cryopreservation; oviducts; puppies; intrauterine administration; spermatozoa; artificial insemination; thawing
DOI:
10.4142/jvs.2007.8.1.75
PubMed:
17322777
PubMed Central:
PMC2872701

12. Bovine viral diarrhea virus (BVDV): Epidemiologic concerns relative to semen and embryos

Author:
Gard, J.A.; Givens, M.D.; Stringfellow, D.A.
Source:
Theriogenology 2007 v.68 no.3 pp. 434-442
ISSN:
0093-691X
Subject:
semen; virus transmission; cryopreservation; cattle; embryo (animal); nuclear transplantation; Bovine viral diarrhea virus 1; embryo transfer; artificial insemination
Abstract:
... Artificial insemination and embryo transfer are used commonly in cattle production and exchange of germplasm between populations of cattle. If properly monitored, assisted reproductive techniques can be used to prevent the spread of infectious agents. However, these techniques potentially represent unnatural routes for transmission of diseases. Bovine viral diarrhea virus (BVDV) is broadly distrib ...
DOI:
10.1016/j.theriogenology.2007.04.003
PubMed:
17588651

13. Calorimetric properties of water and triacylglycerols in fern spores relating to storage at cryogenic temperatures

Author:
Ballesteros, D.; Walters, C.
Source:
Cryobiology 2007 v.55 no.1 pp. 1-9
ISSN:
1090-2392
Subject:
oleic acid; linoleic acid; Pteris vittata; Dryopteris filix-mas; spores; quantitative analysis; cryopreservation; Polystichum setiferum; Thelypteris; triacylglycerols; water content; ferns and fern allies; chemical constituents of plants; germplasm conservation; desiccation (plant physiology)
Abstract:
... Storing spores is a promising method to conserve genetic diversity of ferns ex situ. Inappropriate water contents or damaging effects of triacylglycerol (TAG) crystallization may cause initial damage and deterioration with time in spores placed at -15 °C or liquid nitrogen temperatures. We used differential scanning calorimetry (DSC) to monitor enthalpy and temperature of water and TAG phase trans ...
Handle:
10113/2548
DOI:
10.1016/j.cryobiol.2007.03.006
PubMed:
17553480

14. Comparison of Different Extenders with Defined Protein Composition for Storage of Stallion Spermatozoa at 5°C

Author:
Aurich, C.; Seeber, P.; Müller-Schlösser, F.
Source:
Reproduction in domestic animals 2007 v.42 no.4 pp. 445-448
ISSN:
0936-6768
Subject:
caseinates; cold storage; cryopreservation; dried skim milk; male fertility; protein composition; protein sources; semen; semen extenders; soy protein; spermatozoa; stallions; whey protein
Abstract:
... To maintain the fertility of stallion spermatozoa during cooled storage, extender media are added to semen. In this study, three semen extenders were compared: EquiPro which contains defined caseinates and whey proteins instead of dried skim milk. The extender is provided in dry form and dissolved in distilled water prior to use. EquiPro TM has the same composition as EquiPro but is provided in a ...
DOI:
10.1111/j.1439-0531.2006.00828.x

15. Cryo-scanning electron microscopy (Cryo-SEM) of boar semen frozen in medium-straws and MiniFlatPacks

Author:
Ekwall, H.; Hernandez, M.; Saravia, F.; Rodriguez-Martinez, H.
Source:
Theriogenology 2007 v.67 no.9 pp. 1463-1472
ISSN:
0093-691X
Subject:
boars; semen; cryopreservation; spermatozoa; ultrastructure; scanning electron microscopy; image analysis; semen extenders; sperm motility
Abstract:
... In this study we demonstrate, in the frozen state, the architecture of frozen boar spermatozoa collected from the sperm-rich fraction of ejaculates (n = 13) from four fertile boars packed and split-frozen in medium-straws (MS) and MiniFlatPacks (MFP), cross-sectioned in the frozen state and evaluated by image analysis on images obtained by use of cryo-scanning electron microscopy (Cryo-SEM). The t ...
DOI:
10.1016/j.theriogenology.2007.03.004
PubMed:
17448530

16. Cryogelation phenomena in mixed skim milk powder -- barley β-glucan-polyol aqueous dispersions

Author:
Lazaridou, A.; Biliaderis, C.G.
Source:
Food research international 2007 v.40 no.7 pp. 793-802
ISSN:
0963-9969
Subject:
thermal stability; dispersions; sorbitol; barley; aqueous solutions; glucose; xylose; fructose; molecular weight; microstructure; sucrose; electron microscopy; dried skim milk; beta-glucans; blended foods; deformation; mixing; cryopreservation; freeze-thaw cycles; gelatinization
Abstract:
... The effects of polyols (fructose, glucose, sorbitol, sucrose and xylose) on cryostructurization of barley mixed-linkage (1 leads to 3), (1 leads to 4) β-D-glucans (β-glucans) in the presence of skim milk proteins were investigated; three β-glucan isolates differing in molecular size (apparent molecular weight of 210, 140 and 70 x 10(3) Da) were used. Polyols were incorporated at 15% (w/w) concentr ...
DOI:
10.1016/j.foodres.2007.01.016

17. Cryopreservation and orthotopic transplantation of rat ovaries as a means of gamete banking

Author:
Martina M Dorsch; Dirk Wedekind; Kenji Kamino; Hans J Hedrich
Source:
Laboratory animals 2007 v.41 no.2 pp. 247-254
ISSN:
1758-1117
Subject:
rats; surgical transplantation; cryopreservation
Abstract:
... Besides the exponentially increasing number of mouse strains, the rising number of rat strains, due to the establishment of transgenic and coisogenic strains in this species, surpasses the capacity of most animal houses. Cryopreservation of gametes may be a means of solving these problems. Here we describe an easy and fast method for the cryopreservation and transplantation of frozen–thawed ovarie ...
DOI:
10.1258/002367707780378195
PubMed:
17430624

18. Cryopreservation of European Mouflon (Ovis Gmelini Musimon) Semen During the non-Breeding Season is Enhanced by the Use of Trehalose

Author:
Berlinguer, F.; Leoni, GG; Succu, S.; Mossa, F.; Galioto, M.; Madeddu, M.; Naitana, S.
Source:
Reproduction in domestic animals 2007 v.42 no.2 pp. 202-207
ISSN:
0936-6768
Subject:
Ovis; Pisum sativum; acrosome; agglutinins; cryopreservation; iodides; mouflon; oocytes; rams; semen; thawing; trehalose; viability
Abstract:
... The influence of trehalose on European mouflon spermatozoa cryopreservation during the non-breeding season was tested. Semen was frozen in two different extenders: (a) recommended Tris-based ram extender (CTR); (b) CTR extender supplemented with trehalose 0.147 m m (TRH). Sperm viability and acrosome integrity were assessed using propidium iodide and fluorescein isothiocynate labelled Pisum Sativu ...
DOI:
10.1111/j.1439-0531.2006.00753.x
PubMed:
17348979

19. Cryopreservation of Gladiolus cultivars

Author:
Joung, H.Y.; Cantor, M.; Kamo, K.; Ellis, D.
Source:
Acta horticulturae 2007 v. no.760 pp. 225-231
ISSN:
0567-7572
Subject:
Gladiolus; cryopreservation; cultivars; shoot meristems; breeding lines
Abstract:
... Cryopreservation is an important technique in the long-term storage of plant germplasm. It can be less labor intensive and more reliable for preventing the loss of plants to disease than multiplying plants in the field or in vitro. The objective of this study was to develop a cryopreservation procedure to preserve our transgenic lines of Gladiolus without growing them each year under the special c ...
Handle:
naldc:19998

20. Cryopreservation of Mexican fruit flies by vitrification: Stage selection and avoidance of thermal stress

Author:
Rajamohan, A.; Leopold, R.A.
Source:
Cryobiology 2007 v.54 no.1 pp. 44-54
ISSN:
1090-2392
Subject:
Anastrepha ludens; insect pests; embryo (animal); cryopreservation; embryogenesis; developmental stages; temperature; hatching; heat stress
Abstract:
... This report presents details of a vitrification methodology for the cryopreservation of embryos of the Mexican fruit fly, Anastrepha ludens. The overall summary of the data indicates that selecting the correct developmental stage for cryopreservation is the most important criterion. The key aspect in selection of the correct stage is to balance depletion of the gut yolk content against development ...
Handle:
10113/4358
DOI:
10.1016/j.cryobiol.2006.10.192
PubMed:
17150205

21. Cryopreservation of Primordial Germ Cells (PGCs) from White Leghorn Embryos Using Commercial Cryoprotectants

Author:
Setioko, Argono R.; Tagami, Takahiro; Tase, Hideki; Nakamura, Yoshiaki; Takeda, Kumiko; Nirasawa, Keijiro
ISSN:
1346-7395
Subject:
chickens; viability; germ cells; laboratory techniques; cryopreservation; embryo (animal); cryoprotectants; White Leghorn; blood cells; thawing
Abstract:
... Cryopreservation of poultry semen has been reported, but preservation of female genetic material has not been possible because of the large size of their yolk structure. Cryopreservation of primordial germ cells (PGCs) is an alternative way to preserve both female and male genetic material in chicken. In the present study, fertilized eggs were incubated for about 53 hours to obtain embryos at stag ...

22. Cryopreservation of Sheep Primordial Follicles

Author:
Amorim, CA; Rondina, D.; Lucci, CM; Giorgetti, A.; de Figueiredo, JR; Gonçalves, PBD
Source:
Reproduction in domestic animals 2007 v.42 no.1 pp. 53-57
ISSN:
0936-6768
Subject:
animal ovaries; cryopreservation; dimethyl sulfoxide; ethylene glycol; in vitro culture; propylene glycol; sheep; survival rate
Abstract:
... The aim of this study was to evaluate the efficiency of 1 M dimethylsulphoxide (DMSO), ethylene glycol (EG), propylene glycol (PROH) and glycerol (GLY) to cryopreserve primordial follicles. The first evaluation was performed soon after cryopreservation and the second evaluation after 4 days of in vitro culture, using the cryoprotectants that allowed the higher results (higher follicular survival r ...
DOI:
10.1111/j.1439-0531.2006.00724.x
PubMed:
17214774

23. Cryopreservation of carnation (Dianthus caryophyllus L.) shoot tips by encapsulation-vitrification

Author:
Halmagyi, A.; Deliu, C.
Source:
Scientia horticulturae 2007 v.113 no.3 pp. 300-306
ISSN:
0304-4238
Subject:
floriculture crops; cryopreservation; plant growth substances; explants; artificial seeds; encapsulation; Dianthus caryophyllus; micropropagation; shoot meristems; culture media
DOI:
10.1016/j.scienta.2007.04.002

24. Cryopreservation of epididymal bovine spermatozoa from dead animals and its uses in vitro embryo production

Author:
Martins, C.F.; Rumpf, R.; Pereira, D.C.; Dode, M.N.
Source:
Animal reproduction science 2007 v.101 no.3-4 pp. 326-331
ISSN:
0378-4320
Subject:
cell cleavage; viability; semen; epididymis; bulls; chromatin; cryopreservation; sperm motility; blastocyst; dead animals; male fertility; acrosome; germplasm conservation; in vitro fertilization
DOI:
10.1016/j.anireprosci.2007.01.018

25. Cryopreservation of goat spermatozoa: Comparison of two freezing extenders based on post-thaw sperm quality and fertility rates after artificial insemination

Author:
Dorado, J.; Rodríguez, I.; Hidalgo, M.
Source:
Theriogenology 2007 v.68 no.2 pp. 168-177
ISSN:
0093-691X
Subject:
semen extenders; semen; pregnancy rate; cryopreservation; goats; sperm motility; male fertility; acrosome; artificial insemination
Abstract:
... TRIS-glucose or skim milk extenders are most commonly used for cryopreserving goat sperm. The aim of this study was to compare the ability of two extenders based on TRIS and skimmed milk buffer to maintain sperm viability after cryopreservation. Goat semen samples (n = 110) were frozen with TRIS and with milk extender and thaw. Sperm motion parameters, morphology and acrosomal integrity were asses ...
DOI:
10.1016/j.theriogenology.2007.04.048

26. Cryopreservation of immature and in vitro matured porcine oocytes by solid surface vitrification

Author:
Gupta, M.K.; Uhm, S.J.; Lee, H.T.
Source:
Theriogenology 2007 v.67 no.2 pp. 238-248
ISSN:
0093-691X
Subject:
cytochalasin B; viability; cell cleavage; in vitro culture; cryopreservation; dimethyl sulfoxide; cryoprotectants; ethylene glycol; pretreatment; in vitro fertilization; oocytes; swine
Abstract:
... Cryopreservation of normal, lipid-containing porcine oocytes has had limited practical success. This study used solid surface vitrification (SSV) of immature germinal vesicle (GV) and mature meiosis II (MII) porcine oocytes and evaluated the effects of pretreatment with cytochalasin B, cryoprotectant type (dimethylsulfoxide (DMSO), ethylene glycol (EG), or both), and warming method (two-step versu ...
DOI:
10.1016/j.theriogenology.2006.07.015

27. Cryopreservation of in vitro matured buffalo (Bubalus bubalis) oocytes by minimum volumes vitrification methods

Author:
Gasparrini, B.; Attanasio, L.; De Rosa, A.; Monaco, E.; Di Palo, R.; Campanile, G.
Source:
Animal reproduction science 2007 v.98 no.3-4 pp. 335-342
ISSN:
0378-4320
Subject:
protective effect; solutions; viability; buffaloes; cryopreservation; culture media; cryoprotectants; methodology; blastocyst; Bubalus; yields; embryogenesis; germplasm conservation; oocytes; adverse effects; thawing; in vitro fertilization
DOI:
10.1016/j.anireprosci.2006.04.046

28. Cryopreservation of sperm of the endangered Gila trout, Oncorhynchus gilae

Author:
Fuller, S.A.
Source:
Journal of applied aquaculture 2007 v.19 no.1 pp. 71-79
ISSN:
1045-4438
Subject:
fish culture; Oncorhynchus; trout; endangered species; spermatozoa; cryopreservation; cryoprotectants; germplasm conservation; semen extenders
DOI:
10.1300/J028v19n01_07

29. Cryopreservation of sperm of two European percid species, the pikeperch (Sander lucioperca) and the Volga pikeperch (S. volgensis)

Author:
Z. Bokor; T. Müller; M. Bercsényi; L. Horváth; B. Urbányi; Á. Horváth
Source:
Acta biologica Hungarica 2007 v.58 no.2 pp. 199-207
ISSN:
0236-5383
Subject:
Sander lucioperca; Sander volgensis; cryopreservation; cryoprotectants; dimethyl sulfoxide; fertilizer rates; fish; glucose; hatching; methanol; potassium chloride; spermatozoa; sucrose; urine
Abstract:
... Experiments were carried out on sperm cryopreservation of two European percid fish species, the pikeperch (Sander lucioperca) and the Volga pikeperch (S. volgensis) . Two experiments were conducted on pikeperch sperm. In the first, the effects of three extenders (Glucose, KCl, Sucrose) and two cryoprotectants (dimethyl-sulfoxide: DMSO, methanol: MeOH) were tested on motility and fert ...
DOI:
10.1556/ABiol.58.2007.2.6

30. Cryopreservation of tench, Tinca tinca, sperm: Sperm motility and hatching success of embryos

Author:
Rodina, M.; Gela, D.; Kocour, M.; Alavi, S.M.H.; Hulak, M.; Linhart, O.
Source:
Theriogenology 2007 v.67 no.5 pp. 931-940
ISSN:
0093-691X
Subject:
fertilization (reproduction); Tinca tinca; spermatozoa; cryopreservation; cryoprotectants; dimethyl sulfoxide; propanediols; storage time; sperm motility; fish eggs; embryo (animal); hatching; fish larvae
Abstract:
... The aim of the present study was to elaborate cryopreservation methods for ex situ conservation of tench. Success of cryopreservation was tested during two series of experiments. The first set of experiments studied the effects of two types of cryoprotectants (DMSO and a combination of DMSO with propanediol at ratio 1:1) at concentrations of 8 and 10% and three different equilibration times in two ...
DOI:
10.1016/j.theriogenology.2006.11.007

31. Cryopreservation of the dinoflagellate symbiont of the octocoral Pseudopterogorgia elisabethae

Author:
Lory Z. Santiago-Vázquez; Nealie C. Newberger; Russell G. Kerr
Source:
Marine biology 2007 v.152 no.3 pp. 549-556
ISSN:
0025-3162
Subject:
DNA; DNA primers; Symbiodinium; biomass; cryopreservation; cryoprotectants; ethanol; fluorescence; freezing; methanol; polymerase chain reaction; seawater; symbionts; viability
Abstract:
... In this paper we describe a cryopreservation protocol followed by the culture of Symbiodinium sp. isolated from the Caribbean gorgonian Pseudopterogorgia elisabethae as a potential renewable source of the dinoflagellate symbiont. Four different freezing protocols were designed: a controlled cooling device designed to cool at 1°C/min, a three-step protocol (−20°C for 2 h, −70°C for 2 h, liquid nitr ...
DOI:
10.1007/s00227-007-0704-2

32. Cryopreservation of transgenic rice suspension cells producing recombinant hCTLA4Ig

Author:
Ji-Suk Cho; Seok-Mi Hong; Sung-Yeon Joo; Ji-Seon Yoo; Dong-Il Kim
Source:
Applied microbiology and biotechnology 2007 v.73 no.6 pp. 1470-1476
ISSN:
0175-7598
Subject:
Oryza sativa; T-lymphocytes; antigens; callus; cell viability; cooling; cryopreservation; cryoprotectants; cytotoxicity; dimethyl sulfoxide; freezers; glycerol; humans; mannitol; nitrogen; rice; sodium chloride; sorbitol; storage; sucrose; transgenic plants; trehalose
Abstract:
... Transgenic suspension cells of Oryza sativa L. cv. Dongjin utilized as a host for producing recombinant human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) were preserved in liquid nitrogen (−196 °C) after slow prefreezing in a deep freezer (−70 °C). The development of an optimal procedure for long-term storage was investigated by the addition of various concentrations of cryoprotecta ...
DOI:
10.1007/s00253-006-0627-8
PubMed:
17021872

33. Cryoprotectant optimization for sperm of diploid Pacific oysters by use of commercial dairy sperm freezing facilities

Author:
Dong, Q.; Huang, C.; Eudeline, B.; Tiersch, T.R.
Source:
Aquaculture 2007 v.271 no.1-4 pp. 537-545
ISSN:
0044-8486
Subject:
Crassostrea gigas; oysters; spermatozoa; cryopreservation; cryoprotectants; dimethyl sulfoxide; ethylene glycol; methanol; propylene; freezing; oyster culture
Abstract:
... Although sperm cryopreservation has been practiced in aquatic species for more than 50 years, viable markets for frozen sperm do not currently exist for commercial aquaculture. The present study suggests that the use of commercial cryopreservation facilities used for dairy bulls could be a cost-effective approach to initiate commercialization of frozen sperm in aquaculture, and the oyster industry ...
DOI:
10.1016/j.aquaculture.2007.07.005

34. Cryosurvival of bovine blastocysts is enhanced by culture with trans-10 cis-12 conjugated linoleic acid (10t,12c CLA)

Author:
Pereira, R.M.; Baptista, M.C.; Vasques, M.I.; Horta, A.E.M.; Portugal, P.V.; Bessa, R.J.B.; Silva, J.C.; Pereira, M.S.; Marques, C.C.
Source:
Animal reproduction science 2007 v.98 no.3-4 pp. 293-301
ISSN:
0378-4320
Subject:
protective effect; viability; oocysts; lipid content; microscopy; bioaccumulation; cryopreservation; cattle; embryogenesis; isomers; embryo (animal); bovine serum albumin; conjugated linoleic acid; culture media; in vitro fertilization
DOI:
10.1016/j.anireprosci.2006.03.015

35. Current status in avian semen cryopreservation

Author:
Blesbois, E.
Source:
World's poultry science journal 2007 v.63 no.2 pp. 213-222
ISSN:
0043-9339
Subject:
poultry; semen; cryopreservation
DOI:
10.1017/S0043933907001419

36. Decrease in glutathione (GSH) content in bovine sperm after cryopreservation: Comparison between two extenders

Author:
Stradaioli, G.; Noro, T.; Sylla, L.; Monaci, M.
Source:
Theriogenology 2007 v.67 no.7 pp. 1249-1255
ISSN:
0093-691X
Subject:
chemical composition; semen extenders; freezing; glutathione; bulls; oxidative stress; cryopreservation; acrosome reaction; sperm motility; spermatozoa; thawing
Abstract:
... Growing evidence suggests that among the causes which deteriorate qualitative and functional characteristics of semen after freezing and thawing, there are those linked to oxidative stress and decrease detoxification of antioxidants present in the cells and seminal plasma. Overall, cryopreservation reduces the spermatozoal GSH content. In the present study we assessed the motility, capacitation st ...
DOI:
10.1016/j.theriogenology.2007.01.009

37. Detection of early changes in sperm membrane integrity pre-freezing can estimate post-thaw quality of boar spermatozoa

Author:
Peña, F.J.; Saravia, F.; Johannisson, A.; Wallgren, M.; Rodríguez-Martínez, H.
Source:
Animal reproduction science 2007 v.97 no.1-2 pp. 74-83
ISSN:
0378-4320
Subject:
boars; animal breeding; artificial insemination; cryopreservation; male fertility; screening; cell membranes; freezing quality; viability; differential staining; histopathology; tissue analysis; microstructure; microscopy; spermatozoa
DOI:
10.1016/j.anireprosci.2005.12.014

38. Determinants of sperm quality and fertility in domestic species

Author:
Petrunkina, A.M.; Waberski, D.; Günzel-Apel, A.R.; Töpfer-Petersen, E.
Source:
Reproduction 2007 v.134 no.1 pp. 3-17
ISSN:
1470-1626
Subject:
cell suspension culture; cryopreservation; domestic animals; ejaculation; epithelium; flow cytometry; monitoring; quantitative analysis; spermatozoa
Abstract:
... Fertilization success cannot be attributed solely to the absolute number of vital, motile, morphologically normal spermatozoa inseminated into the female but more especially to their functional competence. A range of in vitro tests has therefore been developed to monitor crucial aspects of sperm function: their ability to adapt to changing osmotic conditions, to bind to the oviductal epithelium, a ...
DOI:
10.1530/REP-07-0046
PubMed:
17641084

39. Determination of Optimal Conditions for Thawing and Diluting Cell-Bound CVI 988 Marek's Disease Vaccine and Stability of the Diluted Vaccine

Author:
Geerlings, H.J.; Hoogendam, A.
Source:
Avian diseases 2007 v.51 no.4 pp. 969-973
ISSN:
0005-2086
Subject:
chickens; viability; chemical concentration; strains; cryopreservation; Meleagrid alphaherpesvirus 1; disease prevention; live vaccines; ambient temperature; water temperature; vaccination; reference standards; Marek disease; chemical treatment; thawing; protective effect
Abstract:
... The cell-associated vaccine strain CVI 988, which is the active component of several commercial Marek's disease vaccines, normally is frozen and stored in liquid nitrogen. In order to ascertain good efficacy of the vaccine, it is crucial that the right procedures are followed for thawing and diluting of the virus. In the study presented here, ampoules containing the frozen product were taken from ...
DOI:
10.1637/7818-121506-RESNOTER2.1
PubMed:
18251410

40. Development and quality of sheep embryos cultured in commercial G1.3/G2.3 sequential media

Author:
Garcia-Garcia, R.M.; Ward, F.; Fair, S.; O'Meara, C.M.; Wade, M.; Duffy, P.; Lonergan, P.
Source:
Animal reproduction science 2007 v.98 no.3-4 pp. 233-240
ISSN:
0378-4320
Subject:
in vivo studies; viability; cryopreservation; sheep; embryo (animal); embryogenesis; culture media; adverse effects; thawing; in vitro fertilization
DOI:
10.1016/j.anireprosci.2006.03.007

41. Development of OPS vitrified pig blastocysts: Effects of size of the collected blastocysts, cryoprotectant concentration used for vitrification and number of blastocysts transferred

Author:
Berthelot, F.; Venturi, E.; Cognié, J.; Furstoss, V.; Martinat-Botté, F.
Source:
Theriogenology 2007 v.68 no.2 pp. 178-185
ISSN:
0093-691X
Subject:
swine; blastocyst; embryo culture; cryopreservation; cryoprotectants; chemical concentration; viability; embryonic mortality; embryo transfer; embryogenesis; pregnancy rate
Abstract:
... Unhatched blastocysts from Large White hyperprolific gilts (n = 103) were identified, measured and vitrified using the Open Pulled Straw (OPS) technique to evaluate the effects of the collected blastocyst size and cryoprotectant concentrations used for vitrification, and the number of embryos transferred per recipient. Vitrified/warmed blastocyst viability was estimated in vitro, as the percentage ...
DOI:
10.1016/j.theriogenology.2007.04.050

42. Development of a cryopreservation protocol for long-term storage of black tiger shrimp (Penaeus monodon) spermatophores

Author:
Vuthiphandchai, V.; Nimrat, S.; Kotcharat, S.; Bart, A.N.
Source:
Theriogenology 2007 v.68 no.8 pp. 1192-1199
ISSN:
0093-691X
Subject:
fertilization (reproduction); Penaeus monodon; shrimp; spermatophores; cryopreservation; cryoprotectants; chemical concentration; frozen storage; storage temperature; storage time; freezing; thawing; spermatozoa; viability; egg hatchability
Abstract:
... The objectives of this study were to determine the effect of cryoprotectants on sperm viability and develop a freezing protocol for long-term storage of P. monodon spermatophores. Spermatophores suspended for 30 min in calcium-free saline (Ca-F saline) containing the cryoprotectants dimethyl sulfoxide (DMSO), ethylene glycol (EG), 1,2-propylene glycol (PG), formamide, and methanol at concentration ...
DOI:
10.1016/j.theriogenology.2007.08.024
PubMed:
17900683

43. Development of a simplified and standardized protocol with potential for high-throughput for sperm cryopreservation in zebrafish Danio rerio

Author:
Yang, H.; Carmichael, C.; Varga, Z.M.; Tiersch, T.R.
Source:
Theriogenology 2007 v.68 no.2 pp. 128-136
ISSN:
0093-691X
Subject:
Danio rerio; fish; spermatozoa; cryopreservation; semen extenders; osmotic pressure; cryoprotectants; toxicity; sperm motility; in vitro fertilization; male fertility; body condition
Abstract:
... Sperm cryopreservation offers potential for long-term storage of genetic resources. However, the current protocols for zebrafish Danio rerio are cumbersome and poorly reproducible. Our objective was to facilitate adoption of cryopreservation by streamlining methods from sperm collection through thawing and use. First, sperm activation was evaluated, and motility was completely inhibited when osmol ...
DOI:
10.1016/j.theriogenology.2007.02.015
PubMed:
17544099
PubMed Central:
PMC2676789

44. Developmental competence of equine oocytes and embryos obtained by in vitro procedures ranging from in vitro maturation and ICSI to embryo culture, cryopreservation and somatic cell nuclear transfer

Author:
Galli, C.; Colleoni, S.; Duchi, R.; Lagutina, I.; Lazzari, G.
Source:
Animal reproduction science 2007 v.98 no.1-2 pp. 39-55
ISSN:
0378-4320
Subject:
cryopreservation; animal reproduction; embryo (animal); biotechnology; somatic cells; embryo culture; nuclear transplantation; cytoplasm; embryogenesis; oocytes; horses; intracytoplasmic sperm injection
DOI:
10.1016/j.anireprosci.2006.10.011

45. Dialysis of boar semen prior to freezing-thawing: Its effects on post-thaw sperm characteristics

Author:
Fraser, L.; Dziekońska, A.; Strzeżek, R.; Strzeżek, J.
Source:
Theriogenology 2007 v.67 no.5 pp. 994-1003
ISSN:
0093-691X
Subject:
semen extenders; boars; cryopreservation; sperm motility; adenosine triphosphate; mitochondria; acrosome; seminal plasma; plasma membrane; dialysis
Abstract:
... Low-molecular weight components of the seminal plasma have a detrimental effect on sperm function. The present study was undertaken to evaluate the effect of the removal of low-molecular weight components by dialysis on sperm characteristics prior to and after freezing. Semen, collected from 5 boars, was extended in Kortowo-3 extender (K-3, Poland) and cooled for 3 h (control non-dialysis) or dial ...
DOI:
10.1016/j.theriogenology.2006.12.002

46. Directional Freezing of Equine Semen in Large Volumes

Author:
Saragusty, J.; Gacitua, H.; Pettit, MT; Arav, A.
Source:
Reproduction in domestic animals 2007 v.42 no.6 pp. 610-615
ISSN:
0936-6768
Subject:
cryopreservation; freezing; industry; nitrogen; pregnancy rate; semen; spermatozoa; stallions; thawing; viability
Abstract:
... Despite its potential impact on the horse industry, sperm cryopreservation is not an established technology throughout the industry, for a number of reasons that include a reduction in pregnancy rate and increased cost per pregnancy. We have evaluated a novel directional freezing technique, based on a multi-thermal gradient (MTG), by comparing it with the conventional, controlled-rate cryopreserva ...
DOI:
10.1111/j.1439-0531.2006.00831.x
PubMed:
17976068

47. Dynamics of sperm DNA fragmentation in domestic animals: II. The stallion

Author:
López-Fernández, C.; Crespo, F.; Arroyo, F.; Fernández, J.L.; Arana, P.; Johnston, S.D.; Gosálvez, J.
Source:
Theriogenology 2007 v.68 no.9 pp. 1240-1250
ISSN:
0093-691X
Subject:
temporal variation; semen; DNA damage; DNA fragmentation; cryopreservation; cooling; storage time; male fertility; spermatozoa; stallions; chromatin
Abstract:
... The mixed success of equine artificial insemination programs using chilled and frozen-thawed semen is most likely associated with the variable response of the sperm cell to the preservation process and the fact that stallions are not selected on the basis of reproductive performance. We propose that the traditional indicators of sperm viability do not fully account for male factor infertility in t ...
DOI:
10.1016/j.theriogenology.2007.08.029
PubMed:
17919715

48. Early pre-pubertal exposure to low-dose oral di(2-ethylhexyl) phthalate does not affect sperm plasma membrane stability, acrosomal integrity or chromatin structure in the post-pubertal boar

Author:
Spjuth, L.; Johannisson, A.; Lundeheim, N.; Rodríguez-Martínez, H.
Source:
Theriogenology 2007 v.68 no.2 pp. 186-195
ISSN:
0093-691X
Subject:
boars; plasticizers; DNA fragmentation; cryopreservation; acrosome reaction; phthalates; dietary exposure; denaturation; spermatozoa; plasma membrane; chromatin
Abstract:
... This study aimed to determine whether pre-pubertal exposure in boars to di(2-ethylhexyl) phthalate (DEHP), a plasticizer reported to have toxic effects on rodent reproduction, would affect the sperm ability to undergo capacitation and acrosome reaction (AR) in vitro or give rise to a higher degree of chromatin instability associated with acid-induced denaturation. Spermatozoa were collected from 1 ...
DOI:
10.1016/j.theriogenology.2007.04.051

49. Effect of Bradykinin on Murrah buffalo (Bubalus bubalis) semen cryopreservation

Author:
Shukla, M.K.; Misra, A.K.
Source:
Animal reproduction science 2007 v.97 no.1-2 pp. 175-179
ISSN:
0378-4320
Subject:
semen extenders; semen; viability; buffaloes; freezing quality; membrane permeability; cryopreservation; bradykinin; males; freeze-thaw cycles; sperm motility; osmoregulation; spermatozoa; artificial insemination; protective effect
DOI:
10.1016/j.anireprosci.2006.02.015

50. Effect of Insemination-Ovulation Interval and Addition of Seminal Plasma on Sow Fertility to Insemination of Cryopreserved Sperm

Author:
Abad, M.; Garcia, JC; Sprecher, DJ; Cassar, G.; Friendship, RM; Buhr, MM; Kirkwood, RN
Source:
Reproduction in domestic animals 2007 v.42 no.4 pp. 418-422
ISSN:
0936-6768
Subject:
insemination; equine chorionic gonadotropin; cryopreservation; sows; farrowing rate; freeze-thaw cycles; Yorkshire (swine breed); seminal plasma; spermatozoa; crossbreds; ovulation; artificial insemination; litter size
Abstract:
... In swine, the use of frozen-thawed (FT) sperm for artificial insemination (AI) is limited because of poor sow fertility, possibly associated with a post-thaw capacitation-like status resulting in fewer fully viable sperm. Sow fertility to AI with FT sperm may improve with deeper deposition of sperm within the female tract, insemination very close to ovulation, or reversal of cryocapacitation by se ...
DOI:
10.1111/j.1439-0531.2006.00801.x
PubMed:
17635780

51. Effect of Meiotic Stages During In Vitro Maturation on the Survival of Vitrified-Warmed Buffalo Oocytes

Author:
Sharma, G. Taru; Loganathasamy, K.
Source:
Veterinary research communications 2007 v.31 no.7 pp. 881-893
ISSN:
0165-7380
Subject:
temporal variation; viability; correlation; buffaloes; cryopreservation; cell culture; biological development; freeze-thaw cycles; oogenesis; metaphase; cell nucleus; meiosis; animal breeding; germplasm conservation; oocytes; females
Abstract:
... The present study was conducted to investigate the effect of meiotic stages during in vitro maturation (IVM) on the survival of vitrified-warmed buffalo oocytes, vitrified at different stages of IVM. Cumulus oocyte complexes obtained from slaughterhouse ovaries were randomly divided into 6 groups: control (non-vitrified, matured for 24 h at 38 ± 1°C, 5% CO₂ in humidified air), and those matured fo ...
DOI:
10.1007/s11259-007-0059-7
PubMed:
17285244

52. Effect of Sperm Cryopreservation and Supplementing Semen Doses with Seminal Plasma on the Establishment of a Sperm Reservoir in Gilts

Author:
Abad, M.; Sprecher, DJ; Ross, P.; Friendship, RM; Kirkwood, RN
Source:
Reproduction in domestic animals 2007 v.42 no.2 pp. 149-152
ISSN:
0936-6768
Subject:
artificial insemination; boars; cooling; cryopreservation; equine chorionic gonadotropin; gilts; human chorionic gonadotropin; insemination; ovulation; seminal plasma; sperm capacitation; spermatozoa
Abstract:
... Frozen-thawed (FT) boar sperm have a reduced fertile life, due in part to a capacitation-like status induced by cooling. Reversal of this cryocapacitation in vitro by exposure to boar seminal plasma (SP) has been demonstrated. The objective of these studies was to determine the effect of SP on the ability of FT sperm to create an oviductal sperm reservoir following artificial insemination (AI). In ...
DOI:
10.1111/j.1439-0531.2006.00744.x
PubMed:
17348971

53. Effect of Sperm Cryopreservation on the European Eel Sperm Viability and Spermatozoa Morphology

Author:
Asturiano, JF; Marco-Jiménez, F.; Peñaranda, DS; Garzón, DL; Pérez, L.; Vicente, JS; Jover, M.
Source:
Reproduction in domestic animals 2007 v.42 no.2 pp. 162-166
ISSN:
0936-6768
Subject:
Anguilla anguilla; cell viability; cryopreservation; developmental stages; dimethyl sulfoxide; proteins; sperm motility; spermatozoa; thawing
Abstract:
... The main objective of the present work was to study the effect of cryopreservation of European eel sperm both on the sperm viability and the spermatozoa head morphology. Spermatozoa morphology was evaluated with computer-assisted morphology analysis after collection in fresh samples, after adding the freezing medium containing dimethyl sulfoxide as cryoprotectant and, finally, after the cryopreser ...
DOI:
10.1111/j.1439-0531.2006.00746.x
PubMed:
17348973

54. Effect of antioxidant supplementation on semen quality and reactive oxygen species of frozen-thawed canine spermatozoa

Author:
Michael, A.; Alexopoulos, C.; Pontiki, E.; Hadjipavlou-Litina, D.; Saratsis, P.; Boscos, C.
Source:
Theriogenology 2007 v.68 no.2 pp. 204-212
ISSN:
0093-691X
Subject:
semen extenders; semen; hydroxyl radicals; cryopreservation; dogs; sperm motility; acrosome; superoxide anion; additives
Abstract:
... The objective of this study was to evaluate post-thaw quality of frozen dog semen processed with diluents containing different antioxidants. Ejaculates were collected, pooled and evaluated for concentration, motility, rapid steady forward movement (RSF movement), viability, acrosomal integrity and by the hypo-osmotic swelling test. Also, superoxide production, hydroxyl radicals and total reactive ...
DOI:
10.1016/j.theriogenology.2007.04.053

55. Effect of coculture with oviduct epithelial cells on viability after transfer of vitrified in vitro produced goat embryos

Author:
Rodríguez-Dorta, N.; Cognie, Y.; Gonzalez, F.; Poulin, N.; Guignot, F.; Touze, J.L.; Baril, G.; Cabrera, F.; Alamo, D.; Batista, M.
Source:
Theriogenology 2007 v.68 no.6 pp. 908-913
ISSN:
0093-691X
Subject:
goats; embryo culture; oviducts; epithelial cells; culture media; cryopreservation; embryogenesis; embryo transfer; viability; embryonic mortality; pregnancy rate; kidding rate
Abstract:
... This study evaluates the effect of coculture with goat oviduct epithelial cells (GOEC) on the pregnancy rate, embryo survival rate and offspring development after direct transfer of vitrified/thawed caprine in vitro produced (IVP) embryos. Oocytes were recovered from slaughterhouse goat ovaries, matured and inseminated with frozen/thawed capacitated semen, and presumptive zygotes were randomly cul ...
DOI:
10.1016/j.theriogenology.2007.07.004

56. Effect of cryopreservation on viability, activation and growth of in situ and isolated ovine early-stage follicles

Author:
Santos, R.R.; Hurk, R. van den; Rodrigues, A.P.R.; Costa, S.H.F.; Martins, F.S.; Matos, M.H.T.; Celestino, J.J.H.; Figueiredo, J.R.
Source:
Animal reproduction science 2007 v.99 no.1-2 pp. 53-64
ISSN:
0378-4320
Subject:
viability; follicular development; freezing; cryopreservation; ovarian follicles; dimethyl sulfoxide; differential staining; cryoprotectants; ethylene glycol; ewes; dyes; thawing; animal breeding; germplasm conservation; culture media; adverse effects; animal tissues; tissue culture
DOI:
10.1016/j.anireprosci.2006.05.013

57. Effect of cysteamine supplementation of in vitro matured bovine oocytes on chilling sensitivity and development of embryos

Author:
Balasubramanian, S.; Rho, G.J.
Source:
Animal reproduction science 2007 v.98 no.3-4 pp. 282-292
ISSN:
0378-4320
Subject:
cold tolerance; viability; oocysts; protective effect; cold injury; cryopreservation; cattle; cooling; culture media; embryo (animal); methodology; storage time; cysteamine; embryogenesis; cold storage; in vitro fertilization
DOI:
10.1016/j.anireprosci.2006.03.011

58. Effect of different procedures of ejaculate collection, extenders and packages on DNA integrity of boar spermatozoa following freezing-thawing

Author:
Fraser, L.; Strzeżek, J.
Source:
Animal reproduction science 2007 v.99 no.3-4 pp. 317-329
ISSN:
0378-4320
Subject:
semen extenders; DNA damage; qualitative analysis; freezing; boars; mitochondria; plasma membrane; chemical treatment; lactose; sperm motility; seminal plasma; adverse effects; DNA fragmentation; cryopreservation; acrosome; artificial insemination; thawing
DOI:
10.1016/j.anireprosci.2006.06.003

59. Effect of egg yolk and seminal plasma heparin binding protein interaction on the freezability of buffalo cauda epididymal spermatozoa

Author:
Singh, L.P.; Harshan, H.M.; Ansari, M.R.
Source:
Animal reproduction science 2007 v.99 no.3-4 pp. 395-400
ISSN:
0378-4320
Subject:
semen extenders; cold injury; freezing; buffaloes; reaction inhibition; freezing quality; frozen storage; cryopreservation; cauda epididymidis; binding proteins; egg yolk; heparin; seminal plasma; spermatozoa; metabolic inhibitors; germplasm conservation; in vitro studies; protective effect
DOI:
10.1016/j.anireprosci.2006.08.018

60. Effect of exogenous calcium on post-thaw growth recovery and subsequent plant regeneration of cryopreserved embryogenic calli of Hevea brasiliensis (Müll. Arg.)

Author:
Lardet, Ludovic; Martin, Florence; Dessailly, Florence; Carron, Marc-Philippe; Montoro, Pascal
Source:
Plant cell reports 2007 v.26 no.5 pp. 559-569
ISSN:
0721-7714
Subject:
Hevea brasiliensis; calcium; calcium chloride; callus; cryopreservation
Abstract:
... A reliable cryopreservation technique was developed for friable embryogenic callus lines of Hevea brasiliensis. The study showed that reducing the CaCl₂ concentration of the pre-culture medium from 9 mM to 1 or 0 mM CaCl₂ before cryopreservation promoted post-thaw callus growth, 1 mM being the optimum CaCl₂ concentration for embryo regeneration. Post-thaw callus proliferation decreased in line wit ...
DOI:
10.1007/s00299-006-0278-3
PubMed:
17186244

61. Effect of lactose and glycerol on the motility, normal apical ridge, chromatin condensation and chromatin stability of frozen boar spermatozoa

Author:
Corcuera, B.D.; Marigorta, P.; Sagüés, A.; Saiz-Cidoncha, F.; Pérez-Gutiérrez, J.F.
Source:
Theriogenology 2007 v.67 no.6 pp. 1150-1157
ISSN:
0093-691X
Subject:
chemical concentration; semen extenders; semen; lactose; boars; cryopreservation; glycerol; sperm motility; cryoprotectants; acrosome; chromatin
Abstract:
... The effect of lactose and glycerol concentration, as well as the equilibration time with glycerol was studied on motility, normal apical ridge (NAR), and chromatin state of boar spermatozoa after the freezing and thawing process. In the first experiment, samples were frozen in first and second extenders containing different concentrations of lactose (11, 12 and 14%). In the second experiment sampl ...
DOI:
10.1016/j.theriogenology.2007.01.002

62. Effect of low density lipoprotein on DNA integrity of freezing-thawing boar sperm by neutral comet assay

Author:
Jiang, Z.L.; Li, Q.W.; Li, W.Y.; Hu, J.H.; Zhao, H.W.; Zhang, S.S.
Source:
Animal reproduction science 2007 v.99 no.3-4 pp. 401-407
ISSN:
0378-4320
Subject:
protective effect; freezing; cold injury; DNA damage; boars; temporal variation; cryopreservation; assays; freeze-thaw cycles; low density lipoprotein; spermatozoa; animal breeding; germplasm conservation; additives
DOI:
10.1016/j.anireprosci.2006.08.022

63. Effect of macromolecules in solutions for vitrification of mature bovine oocytes

Author:
Checura, C.M.; Seidel, G.E. Jr.
Source:
Theriogenology 2007 v.67 no.5 pp. 919-930
ISSN:
0093-691X
Subject:
cattle; oocytes; cryopreservation; solutions; culture media; chemical composition; cryoprotectants; blood serum; in vitro fertilization; embryo culture; embryogenesis; blastocyst
Abstract:
... This study was designed to evaluate vitrification procedures for in vitro matured bovine oocytes for efficient blastocyst production after warming, IVF and culture. A second goal was to replace serum as the macromolecular component of the vitrification solution, without compromising efficacy. The first experiment compared two containers, open pulled straws (OPS) versus cryoloops, and two vitrifica ...
DOI:
10.1016/j.theriogenology.2006.09.044

64. Effect of moisture content of tomato pollen stored cryogenically on in vitro germination, fecundity and respiration during pollen tube growth

Author:
C. Karipidis; C. Lympios; H. C. Passam; D. Savvas
Source:
Journal of horticultural science & biotechnology 2007 v.82 no.1 pp. 29-34
ISSN:
2380-4084
Subject:
Solanum lycopersicum var. lycopersicum; air; cryopreservation; fecundity; fruits; oxygen; oxygen consumption; pollen; pollen tubes; pollination; rehydration; relative humidity; seeds; thawing; tomatoes; viability; water content
Abstract:
... The moisture content (MC) of tomato pollen was conditioned to a range between 3.8% and 53% of fresh weight by equilibration in air with a relative humidity (RH) ranging from 0 – 100%. After storage in liquid N (–196°C) for 5 d, followed by thawing and rehydration, germination was measured in vitro on a semi-solid substrate. Pollen with a MC of 6.8% or 9.3%, which was achieved by exposure to 50% or ...
DOI:
10.1080/14620316.2007.11512195

65. Effect of post-thaw dilution with autologous prostatic fluid on dog semen motility and sperm acrosome status

Author:
Rota, A.; Milani, C.; Romagnoli, S.
Source:
Theriogenology 2007 v.67 no.3 pp. 520-525
ISSN:
0093-691X
Subject:
semen extenders; semen; longevity; cryopreservation; dogs; sperm motility; body fluids; prostate gland; acrosome
Abstract:
... The aim of this study was to evaluate the effect of post-thaw dilution with autologous prostatic fluid on motility parameters, longevity and acrosome status of frozen-thawed dog spermatozoa. After semen collection, seminal plasma was separated by centrifugation and stored frozen until use. Sperm pellets were diluted in two steps with an egg yolk-Tris extender to a final concentration of 5% glycero ...
DOI:
10.1016/j.theriogenology.2006.09.001
PubMed:
17010416

66. Effect of the luteinizing hormone on embryo production in superovulated rabbit does

Author:
Salvetti, P.; Theau-Clément, M.; Beckers, J.F.; Hurtaud, J.; Guérin, P.; Neto, V.; Falières, J.; Joly, T.
Source:
Theriogenology 2007 v.67 no.6 pp. 1185-1193
ISSN:
0093-691X
Subject:
females; rabbits; superovulation; luteinizing hormone; follicle-stimulating hormone; corpus luteum; embryo (animal); viability; embryo culture; embryogenesis; cryopreservation
Abstract:
... For most domestic animals, the responses to superovulation treatments are not controlled as a consequence of the lack of knowledge on exogenous gonadotrophins effects on the ovarian function. The role of luteinizing hormone (LH) on the number and quality of embryos produced was evaluated on rabbit does superovulated with porcine FSH (pFSH). Parameters of embryos recovery, in vitro and in vivo embr ...
DOI:
10.1016/j.theriogenology.2007.01.013

67. Effect of vitrification solutions and cooling upon in vitro matured prepubertal ovine oocytes

Author:
Succi, S.; Leoni, G.G.; Berlinguer, F.; Madeddu, M.; Bebbere, D.; Mossa, F.; Bogliolo, l.; Ledda, S.; Naitana, S.
Source:
Theriogenology 2007 v.68 no.1 pp. 107-114
ISSN:
0093-691X
Subject:
cryopreservation; sheep; chromosome morphology; cryoprotectants; maturation-promoting factor; microtubules; chromatin; embryogenesis; oocytes; in vitro fertilization
Abstract:
... The vitrification procedure effects on molecular and cytoskeletal components and on developmental ability of in vitro matured prepubertal ovine oocytes were evaluated. MII oocytes were divided into three groups: (1) vitrified in cryoloops (VTR); (2) exposed to vitrification solutions and rehydrated without being plunged into liquid nitrogen (EXP); (3) without further treatment as a control (CTR). ...
DOI:
10.1016/j.theriogenology.2007.04.035

68. Effective use of encapsulation-dehydration technique in cryopreserving somatic embryos of butterfly pea (Clitoria ternatea L.)

Author:
Nair, D.S.; Reghunath, B.R.
Source:
Journal of herbs, spices & medicinal plants 2007 v.13 no.3 pp. 83-96
ISSN:
1049-6475
Subject:
Clitoria ternatea; ornamental plants; medicinal plants; nursery crops; specialty crops; cryopreservation; embryo (plant); somatic embryogenesis; plant genetic resources; germplasm; callus; culture media; micropropagation; 2,4-D; benzyladenine; germination; gibberellic acid; microencapsulation; mortality; viability; regrowth
DOI:
10.1300/J044v13n03_07

69. Effects of Cryopreservation on Bull Spermatozoa Distribution in Morphometrically Distinct Subpopulations

Author:
Rubio-Guillén, J.; González, D.; Garde, JJ; Esteso, MC; Fernández-Santos, MR; Rodríguez-Gíl, JE; Madrid-Bury, N.; Quintero-Moreno, A.
Source:
Reproduction in domestic animals 2007 v.42 no.4 pp. 354-357
ISSN:
0936-6768
Subject:
bulls; cryopreservation; freezing; glycerol; morphometry; nitrogen; semen; spermatozoa; thawing; vapors
Abstract:
... Assisted sperm morphometry analysis (ASMA) was used in this study to determine the effects of cryopreservation on bull spermatozoa distribution in morphometrically distinct subpopulations. Ejaculates were collected from five bulls and were divided. One portion was diluted at 30°C in a skim milk-egg yolk medium, containing glycerol. A microscope slide was prepared from single extended sperm samples ...
DOI:
10.1111/j.1439-0531.2006.00788.x
PubMed:
17635770

70. Effects of cryopreservation on semen quality and the expression of sperm membrane hexose transporters in the spermatozoa of Iberian pigs

Author:
Sancho, S.; Casas, I.; Ekwall, H.; Saravia, F.; Rodriguez-Martinez, H.; Rodriguez-Gil, J.E.; Flores, E.; Pinart, E.; Briz, M.; Garcia-Gil, N.; Bassols, J.; Pruneda, A.; Bussalleu, E.; Yeste, M.; Bonet, S.
Source:
Reproduction 2007 v.134 no.1 pp. 111-121
ISSN:
1470-1626
Subject:
Iberian (swine breed); Western blotting; boars; cooling; cryopreservation; freeze-thaw cycles; freezing; glucose transporters; glycerol; plasma membrane; polyacrylamide gel electrophoresis; polyclonal antibodies; rabbits; receptors; scanning electron microscopy; semen; sperm motility; spermatozoa; thawing
Abstract:
... This study evaluated the effects of cooling, freezing and thawing on the plasma membrane integrity, kinetics and expression of two sugar transporters glucose transporter-3 and -5 (GLUT-3 and GLUT-5) in spermatozoa from Iberian boars. Semen samples were collected twice weekly from eight young, fertile Iberian boars of the 'Entrepelado' and 'Lampiño' breeds. The samples were suspended in a commercia ...
DOI:
10.1530/REP-07-0118
PubMed:
17641093

71. Effects of cryopreservation on sperm quality, nuclear DNA integrity, in vitro fertilization, and in vitro embryo development in the mouse

Author:
Yildiz, Cengiz; Ottaviani, Palma; Law, Napoleon; Ayearst, Renise; Liu, Ling; McKerlie, Colin
Source:
Reproduction 2007 v.133 no.3 pp. 585-595
ISSN:
1470-1626
Subject:
DNA; DNA fragmentation; animal models; blastocyst; chromatin; cryopreservation; cryoprotectants; embryogenesis; ethylene glycol; freezing; fructose; glucose; glycerol; human diseases; in vitro fertilization; mice; nuclear genome; plasma membrane; propylene glycol; protective effect; pyruvic acid; raffinose; research programs; skim milk; sodium; spermatozoa; thawing
Abstract:
... Efficient freezing, archiving, and thawing of sperm are essential techniques to support large scale research programs using mouse models of human disease. The purpose of this study was to investigate the effects of variable combinations and concentrations of cryoprotectants on sperm-assessment parameters of frozen-thawed mouse sperm in order to optimize cryopreservation protocols. Sperm was frozen ...
DOI:
10.1530/REP-06-0256
PubMed:
17379653

72. Effects of platelet-activating factor and platelet-activating factor: acetylhydrolase on in vitro post-thaw boar sperm parameters

Author:
Bathgate, R.; Maxwell, W.M.C.; Evans, G.
Source:
Theriogenology 2007 v.67 no.4 pp. 886-892
ISSN:
0093-691X
Subject:
semen extenders; semen; boars; cryopreservation; platelet-activating factor; sperm motility; cryoprotectants; hydrolases; acrosome; additives
Abstract:
... Cryopreservation of boar sperm compromises fertility after thawing by reducing sperm longevity and inducing acrosome reaction-like changes. In an attempt to improve the post-thaw motility and acrosome integrity of boar sperm, semen was frozen using a modified Westendorf method in which the medium was supplemented with either platelet-activating factor (PAF) or a recombinant platelet-activating fac ...
DOI:
10.1016/j.theriogenology.2006.09.043

73. Effects of sperm concentration at semen collection and storage period of frozen semen on dairy cow conception

Author:
Haugan, T.; Gröhn, Y.T.; Kommisrud, E.; Ropstad, E.; Reksen, O.
Source:
Animal reproduction science 2007 v.97 no.1-2 pp. 1-11
ISSN:
0378-4320
Subject:
dairy cows; viability; semen extenders; frozen storage; cryopreservation; conception; storage time; female fertility; probability analysis; male fertility; reproductive efficiency; dairy bulls; seminal plasma; spermatozoa; artificial insemination
DOI:
10.1016/j.anireprosci.2005.12.010

74. Efficient genome-wide mutagenesis of zebrafish genes by retroviral insertions

Author:
Wang, Dongmei; Jao, Li-En; Zheng, Naizhong; Dolan, Kyle; Ivey, Jessica; Zonies, Seth; Wu, Xiaolin; Wu, Kangmai; Yang, Hongbo; Meng, Qingchao; Zhu, Zuoyan; Zhang, Bo; Lin, Shuo; Burgess, Shawn M.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2007 v.104 no.30 pp. 12428-12433
ISSN:
0027-8424
Subject:
messenger RNA; probability; cryopreservation; mutagenesis; inbreeding; mutagenicity; spermatozoa; Murine leukemia virus; fish; progeny; Danio rerio; exons; introns
Abstract:
... Using a combination of techniques we developed, we infected zebrafish embryos using pseudotyped retroviruses and mapped the genomic locations of the proviral integrations in the F₁ offspring of the infected fish. From F₁ fish, we obtained 2,045 sequences representing 933 unique retroviral integrations. A total of 599 were mappable to the current genomic assembly (Zv6), and 233 of the integrations ...
DOI:
10.1073/pnas.0705502104
PubMed:
17640903
PubMed Central:
PMC1924792

75. Ejaculate and type of freezing extender affect rates of fertilization of horse oocytes in vitro

Author:
Roasa, L.M.; Choi, Y.H.; Love, C.C.; Romo, S.; Varner, D.D; Hinrichs, K.
Source:
Theriogenology 2007 v.68 no.4 pp. 560-566
ISSN:
0093-691X
Subject:
horses; ejaculation; semen; spermatozoa; cryopreservation; chemical composition; semen extenders; bovine serum albumin; storage time; oocytes; in vitro fertilization
Abstract:
... In vitro fertilization (IVF) was performed on in vitro-matured equine oocytes in three experiments. Frozen-thawed sperm were prepared using swim-up separation and heparin treatment. In Experiment 1, fertilization was achieved with sperm from only one frozen ejaculate of four obtained from the same stallion. Within this ejaculate, fertilization rates were higher with fresh media, as compared to med ...
DOI:
10.1016/j.theriogenology.2007.04.057

76. Encapsulation of moss buds: an efficient method for the in vitro conservation and regeneration of the endangered moss Splachnum ampullaceum

Author:
Mallón, Rubén; Barros, Paula; Luzardo, Asteria; González, María Luz
Source:
Plant cell, tissue, and organ culture 2007 v.88 no.1 pp. 41-49
ISSN:
0167-6857
Subject:
temporal variation; in vitro regeneration; plant development; buds; in vitro culture; gametophytes; long term experiments; cryopreservation; storage time; encapsulation; regrowth; Bryopsida; germplasm conservation; mortality; cold storage
Abstract:
... In vitro culture techniques are usually employed for ex situ conservation of endangered plant species. However, encapsulation to preserve threatened bryophytes is scarcely used, and only as a pretreatment prior to cryopreservation. In our study, two different methods of germplasm conservation, involving calcium-alginate encapsulation of moss material, were assessed. The plant material used was gam ...
DOI:
10.1007/s11240-006-9176-1

77. Evaluation of a cushioned method for centrifugation and processing for freezing boar semen

Author:
Matás, C.; Decuadro, G.; Martínez-Miró, S.; Gadea, J.
Source:
Theriogenology 2007 v.67 no.5 pp. 1087-1091
ISSN:
0093-691X
Subject:
boars; semen; cryopreservation; centrifugation; methodology; benzoic acids; freezing; spermatozoa; viability; sperm motility
Abstract:
... The purpose of this investigation was to evaluate the use of an iodixanol cushion during centrifugation on sperm recovery and yield after centrifugation (sperm recovery, sperm motility, viability, membrane lipid disorder, acrosome reaction and ROS generation); and to investigate how this procedure affects sperm function after freezing-thawing (sperm motility, membrane lipid disorder, acrosomal sta ...
DOI:
10.1016/j.theriogenology.2006.11.010

78. Experimental study on the freezing characteristics of four kinds of vegetables

Author:
Haiying, W.; Shaozhi, Z.; Guangming, C.
Source:
Lebensmittel-Wissenschaft + Technologie 2007 v.40 no.6 pp. 1112-1116
ISSN:
0023-6438
Subject:
mushrooms; cauliflower; navy beans; peas; pods; food preservation; freezing; pretreatment; glass transition temperature; freezing point; ice nucleation; differential scanning calorimetry; frozen vegetables; cryopreservation
DOI:
10.1016/j.lwt.2006.06.001

79. Expression of the GM-CSF receptor in ovine spermatozoa: GM-CSF effect on sperm viability and motility of sperm subpopulations after the freezing-thawing process

Author:
Rodríguez-Gil, J.E.; Silvers, G.; Flores, E.; Palomo, M.J.; Ramírez, A.; Montserrat Rivera, M.; Castro, M.; Brito, M.; Bücher, D.; Correa, J.
Source:
Theriogenology 2007 v.67 no.8 pp. 1359-1370
ISSN:
0093-691X
Subject:
freezing; viability; cryopreservation; granulocyte-macrophage colony-stimulating factor; sperm motility; gene expression; thawing; spermatozoa; receptors; rams; protective effect
Abstract:
... The granulocyte-macrophage colony stimulating factor (GM-CSF) is a pleiotropic cytokine capable of stimulating proliferation, maturation and function of haematopoietic cells. Receptors for this cytokine are composed of two subunits, alpha and beta, and are expressed in myeloid progenitors and mature mononuclear phagocytes, monocytes, eosinophils and neutrophils, as well as in other non-haematopoie ...
DOI:
10.1016/j.theriogenology.2007.02.008
PubMed:
17408732

80. Extender osmolality and sugar supplementation exert a complex effect on the cryopreservation of Iberian red deer (Cervus elaphus hispanicus) epididymal spermatozoa

Author:
Fernández-Santos, M.R.; Martínez-Pastor, F.; García-Macías, V.; Esteso, M.C.; Soler, A.J.; Paz, P. de; Anel, L.; Garde, J.J.
Source:
Theriogenology 2007 v.67 no.4 pp. 738-753
ISSN:
0093-691X
Subject:
Cervus elaphus; deer; epididymis; spermatozoa; cryopreservation; semen extenders; osmotic pressure; additives; sugars; cryoprotectants; viability; sperm motility; mathematical models
Abstract:
... We have carried out two experiments to study the cryobiology of red deer epididymal spermatozoa and to improve freezing extenders: (1) effect of extender (Tris-citrate-fructose) osmolality (300-600 mOsm/kg), and (2) effect of sugar (0.4 M) supplementation to the extender (no sugar, glucose, fructose, mannose, sucrose, maltose, threalose and raffinose). Sperm quality was assessed pre-freezing, post ...
DOI:
10.1016/j.theriogenology.2006.10.005

81. Field performance characterization of strawberry (Fragaria x ananassa Duch.) plants derived from cryopreserved apices

Author:
Medina, J.J.; Clavero-Ramírez, I.; González-Benito, M.E.; Gálvez-Farfán, J.; López-Aranda, J.M.; Soria, C.
Source:
Scientia horticulturae 2007 v.113 no.1 pp. 28-32
ISSN:
0304-4238
Subject:
Fragaria ananassa; strawberries; cryopreservation; shoot meristems; apical meristems; in vitro regeneration; crop yield; field experimentation; agronomic traits; fruit quality; fruit set; micropropagation; phenotype; Spain
DOI:
10.1016/j.scienta.2007.01.030

82. Flow cytometry and ultrastructure of cryopreserved red seabream (Pagrus major) sperm

Author:
Liu, Q.H.; Li, J.; Zhang, S.C.; Xiao, Z.Z.; Ding, F.H.; Yu, D.D.; Xu, X.Z.
Source:
Theriogenology 2007 v.67 no.6 pp. 1168-1174
ISSN:
0093-691X
Subject:
fertilization (reproduction); Pagrus major; spermatozoa; cryopreservation; flow cytometry; ultrastructure; sperm motility; plasma membrane; mitochondria; hatching; bream; marine fish
Abstract:
... The objectives were to assess motility, fertilizing capacity, structural integrity, and mitochondrial function in fresh versus frozen-thawed (15% DMSO was used as a cryoprotectant) sperm from red seabream (Pagrus major). Mean (±S.D.) rates of motility, fertilization and hatching of frozen-thawed sperm were 81.0 ± 5.4, 92.8 ± 1.9, and 91.8 ± 5.2%, respectively; for fresh sperm, they were 87.5 ± 7.7 ...
DOI:
10.1016/j.theriogenology.2006.12.013

83. Formation of Specialized Propagules Resistant to Desiccation and Cryopreservation in the Threatened Moss Ditrichum plumbicola (Ditrichales, Bryopsida)

Author:
Rowntree, J.K.; Duckett, J.G.; Mortimer, C.L.; Ramsay, M.M.; Pressel, S.
Source:
Annals of botany 2007 v.100 no.3 pp. 483-496
ISSN:
0305-7364
Subject:
Bryopsida; abscisic acid; asexual reproduction; cryopreservation; droplets; encapsulation; environmental factors; freezing; light microscopy; lipids; mosses and liverworts; nitrogen; sodium alginate; sucrose; vacuoles
Abstract:
... BACKGROUND AND AIMS: Successful cryopreservation of bryophytes is linked to intrinsic desiccation tolerance and survival can be enhanced by pre-treatment with abscisic acid (ABA) and sucrose. The pioneer moss Ditrichum plumbicola is naturally subjected to desiccation in the field but showed unexpectedly low survival of cryopreservation, as well as a poor response to pre-treatment. The effects of t ...
DOI:
10.1093/aob/mcm141
PubMed:
17666410
PubMed Central:
PMC2533608

84. Full-term delivery of river buffalo calves (2n = 50) from in vitro-derived vitrified embryos by swamp buffalo recipients (2n = 48)

Author:
Hufana-Duran, D.; Pedro, P.B.; Venturina, H.V.; Duran, P.G.; Cruz, L.C.
Source:
Livestock science 2007 v.107 no.2-3 pp. 213-219
ISSN:
1878-0490
Subject:
breed differences; semen; calving rate; buffaloes; cryopreservation; chromosome number; fetal death; genetic improvement; dystocia; embryo transfer; in vitro fertilization; Philippines
DOI:
10.1016/j.livsci.2006.09.022

85. Gene expression and development of mouse zygotes following droplet vitrification

Author:
Dhali, A.; Anchamparuthy, V.M.; Butler, S.P.; Pearson, R.E.; Mullarky, I.K.; Gwazdauskas, F.C.
Source:
Theriogenology 2007 v.68 no.9 pp. 1292-1298
ISSN:
0093-691X
Subject:
cryopreservation; rapid methods; mice; zygote; blastocyst; gene expression; transcription (genetics); apoptosis; cell cleavage
Abstract:
... The concept of ultra-rapid vitrification has emerged in recent years; the accelerated cooling rate reduced injury attributed to cryopreservation and improved post-freezing developmental competence of vitrified oocytes and embryos. The objectives of the present study were to develop a simple and effective ultra-rapid vitrification method (droplet vitrification) and evaluate its effects on post-thaw ...
DOI:
10.1016/j.theriogenology.2007.08.030
PubMed:
17915304

86. Heparin and quercitin generate differential metabolic pathways that involve aminotransferases and LDH-X dehydrogenase in cryopreserved bovine spermatozoa

Author:
Cordoba, M.; Pintos, L.N.; Beconi, M.T.
Source:
Theriogenology 2007 v.67 no.3 pp. 648-654
ISSN:
0093-691X
Subject:
quercitrin; aspartate transaminase; bulls; cryopreservation; malate dehydrogenase; alanine transaminase; biochemical pathways; heparin; spermatozoa; enzyme activity; amino acid metabolism; lactate dehydrogenase
Abstract:
... Heparin and quercetin induce capacitation in spermatozoa through membrane receptor binding and inhibition of Ca-ATPase of the plasma membrane, respectively. Although capacitation is energy intensive, ammonia from amino acid metabolism can inhibit respiration and Krebs cycle activity. The objective was to determine activities of key enzymes in bull spermatozoa that contribute to the redox state and ...
DOI:
10.1016/j.theriogenology.2006.09.041
PubMed:
17084443

87. Highly efficient vitrification for cryopreservation of human oocytes and embryos: The Cryotop method

Author:
Kuwayama, M.
Source:
Theriogenology 2007 v.67 no.1 pp. 73-80
ISSN:
0093-691X
Subject:
human reproduction; humans; cryopreservation; laboratory techniques; biotechnology; blastocyst; assisted reproductive technologies; oocytes
Abstract:
... Vitrification is frequently referred to as a novel technology of cryopreservation in embryology, although some young embryologists were born after its first successful application. Unfortunately, in spite of the accumulated evidence regarding its enormous potential value, most domestic animal and human laboratories use exclusively the traditional slow-rate freezing with its compromised efficiency ...
DOI:
10.1016/j.theriogenology.2006.09.014

88. Immature bovine oocyte cryopreservation: Comparison of different associations with ethylene glycol, glycerol and dimethylsulfoxide

Author:
Yamada, C.; Caetano, H.V.A.; Simões, R.; Nicacio, A.C.; Feitosa, W.B.; Assumpção, M.E.O.D.; Visintin, J.A.
Source:
Animal reproduction science 2007 v.99 no.3-4 pp. 384-388
ISSN:
0378-4320
Subject:
cryopreservation; morphogenesis; dimethyl sulfoxide; glycerol; adverse effects; cryoprotectants; ethylene glycol; cows; toxicity; animal breeding; germplasm conservation; oocytes; chemical treatment
DOI:
10.1016/j.anireprosci.2006.07.001

89. Impaired Expression of Perforin and Granulysin in CD8⁺ T Cells at the Site of Infection in Human Chronic Pulmonary Tuberculosis

Author:
Andersson, Jan; Samarina, Arina; Fink, Joshua; Rahman, Sayma; Grundström, Susanna
Source:
Infection and immunity 2007 v.75 no.11 pp. 5210-5222
ISSN:
0019-9567
Subject:
immunity; microscopy; human diseases; messenger RNA; image analysis; nitric oxide synthase; quantitative polymerase chain reaction; cryopreservation; macrophages; inflammation; tuberculosis; patients; lungs; CD8-positive T-lymphocytes
Abstract:
... Protective immunity in tuberculosis is dependent on the coordinated release of cytolytic effector molecules from effector T cells and the subsequent granule-associated killing of infected target cells. In this study, we investigated the expression of cytolytic (perforin and granzyme A) and antimicrobial (granulysin) molecules at the single-cell level in cryopreserved lung tissue from patients with ...
DOI:
10.1128/IAI.00624-07
PubMed:
17664265
PubMed Central:
PMC2168267

90. Importation of in vitro-produced Bubalus bubalis embryos from Italy into the United States: A case report

Author:
BonDurant, R.H.; Drost, M.; Zambrano-Varon, J.; Campanile, G.; Gasparrini, B.; Zicarelli, L.
Source:
Theriogenology 2007 v.68 no.3 pp. 454-460
ISSN:
0093-691X
Subject:
buffaloes; embryo (animal); imports; embryo culture; cryopreservation; embryo transfer; import policies; trade regulations; Animal and Plant Health Inspection Service; Italy; United States
Abstract:
... On December 19, 2005, 14 in vitro-fertilized water buffalo (Bubalus bubalis) embryos, which had been cryopreserved by vitrification, were thawed and transferred into B. bubalis recipients in California. The embryos had been produced in Italy, following transvaginal oocyte pickup (TVOPU), with subsequent in vitro maturation, insemination, and culture. This case study relates our experience in meeti ...
DOI:
10.1016/j.theriogenology.2007.05.049
PubMed:
17582487

91. Improved Culture of Histomonas Meleagridis in a Modification of Dwyer Medium

Author:
Heijden, H.M.F.J. van der; Landman, W.J.M.
Source:
Avian diseases 2007 v.51 no.4 pp. 986-988
ISSN:
0005-2086
Subject:
chickens; viability; parasites; rice; animal tissue extracts; blood serum; cryopreservation; turkeys; Histomonas meleagridis; histomoniasis; embryo (animal); cryoprotectants; in vitro studies; yields; microbial resuscitation; powders; culture media; horses
Abstract:
... Dwyer medium is the most frequently employed culture medium for Histomonas meleagridis. Both for subculturing and for resuscitation of H. meleagridis from storage in liquid nitrogen, modified Dwyer medium with an increased rice powder concentration (0.8%) and no chicken embryo extract proved superior to Dwyer standard medium, with threefold (106.3vs. 105.8 histomonads/ml) to 10-fold (106.7vs. 105. ...
DOI:
10.1637/8018-051007-REVIEWR.1
PubMed:
18251414

92. Improvement of freezing and oxidative stress tolerance in Saccharomyces cerevisiae by taurine

Author:
Honjoh, K.; Machida, T.; Nishi, K.; Matsuura, K.; Soli, K.W.; Sakai, T.; Ishikawa, H.; Matsumoto, K.; Miyamoto, T.; Iio, M.
Source:
Food science and technology research 2007 v.13 no.2 pp. 145-154
ISSN:
1344-6606
Subject:
taurine; Saccharomyces cerevisiae; bakers yeast; food preservation; cryopreservation; cryoprotectants; sodium chloride; proline; freezing; cold tolerance; oxidative stress
DOI:
10.3136/fstr.13.145

93. Improving genetic transformation of European chestnut and cryopreservation of transgenic lines

Author:
Corredoira, E.; San-José, M. C.; Vieitez, A. M.; Ballester, A.
Source:
Plant cell, tissue, and organ culture 2007 v.91 no.3 pp. 281-288
ISSN:
0167-6857
Subject:
genetic transformation; gene transfer; forest trees; transgenes; cryopreservation; transgenic plants; Agrobacterium radiobacter; explants; gene expression; Castanea sativa; beta-glucuronidase; somatic embryos
Abstract:
... The aim of the present work was to study the effect of the developmental stage of the somatic embryos and of the genotype on the genetic transformation of embryogenic lines of European chestnut (Castanea sativa Mill.) and the cryopreservation of the embryogenic lines that are generated. As an initial source of explants in the transformation experiments, it was found that the use of somatic embryos ...
DOI:
10.1007/s11240-007-9295-3

94. In vitro germination and seedling development of cryopreserved Dendrobium hybrid mature seeds

Author:
Vendrame, W.A.; Carvalho, V.S.; Dias, J.M.M.
Source:
Scientia horticulturae 2007 v.114 no.3 pp. 188-193
ISSN:
0304-4238
Subject:
Dendrobium; seeds; seed germination; in vitro culture; cryopreservation; seedling growth; seedlings; viability; cultivars; genetic variation; roots; rooting; shoots; plant nutrition; acclimation; plant diseases and disorders
DOI:
10.1016/j.scienta.2007.06.006

95. In vitro production of bovine embryos in medium supplemented with a serum replacer: Effects on blastocyst development, cryotolerance and survival to term

Author:
Moore, K.; Rodríguez-Sallaberry, C.J.; Kramer, J.M.; Johnson, S.; Wroclawska, E.; Goicoa, S.; Niasari-Naslaji, A.
Source:
Theriogenology 2007 v.68 no.9 pp. 1316-1325
ISSN:
0093-691X
Subject:
viability; pregnancy rate; calving rate; blood serum; cryopreservation; cattle; culture media; blastocyst; embryogenesis; embryo culture; oocytes; embryonic mortality; in vitro fertilization
Abstract:
... In this study, we evaluated a serum replacer (SR; Knockout SR, Invitrogen) in our in vitro culture systems. We hypothesized that SR would benefit bovine embryo development, since SR supported survival of embryonic stem cells (which originate from embryos). Experiment 1 compared oocyte maturation with SR versus fetal bovine serum (FBS). Following fertilization, blastocyst development was lower for ...
DOI:
10.1016/j.theriogenology.2007.08.034
PubMed:
17931700

96. In-straw cryoprotectant dilution of IVP bovine blastocysts vitrified in hand-pulled glass micropipettes

Author:
Vieira, A.D.; Forell, F.; Feltrin, C.; Rodrigues, J.L.
Source:
Animal reproduction science 2007 v.99 no.3-4 pp. 377-383
ISSN:
0378-4320
Subject:
in vivo studies; viability; pregnancy rate; cryopreservation; embryo implantation; cryoprotectants; ethylene glycol; blastocyst; cows; in vitro studies; embryo transfer; animal breeding; chemical treatment; in vitro fertilization
DOI:
10.1016/j.anireprosci.2006.06.010

97. Incorporation of taurine and hypotaurine did not improve the efficiency of the Uppsala Equex II extender for dog semen freezing

Author:
Martins-Bessa, A.; Rocha, A.; Mayenco-Aguirre, A.
Source:
Theriogenology 2007 v.68 no.8 pp. 1088-1096
ISSN:
0093-691X
Subject:
chemical concentration; semen extenders; semen; viability; taurine; cryopreservation; dogs; amino acid derivatives; sperm motility; mitochondria; acrosome; additives
Abstract:
... The working hypothesis of the present study was that supplementation of the Uppsala Equex II (UE) extender with the amino acid (AA), taurine (T) and hypotaurine (H) would improve dog sperm post-thaw quality, as previously seen for ram and bull semen, respectively. Five pools from 15 ejaculates of 15 dogs were used. Each AA was added to the UE extender at a concentration of 25, 50 and 75 mM. Amino ...
DOI:
10.1016/j.theriogenology.2007.07.015
PubMed:
17875316

98. Induction and cryopreservation of embryogenic cultures from nucelli and immature cotyledon cuts of mango (Mangifera indica L. var Zihua)

Author:
Wu, Yong-Jie; Huang, Xue-Lin; Chen, Qi-Zhu; Li, Xiao-Ju; Engelmann, Florent
Source:
Plant cell reports 2007 v.26 no.2 pp. 161-168
ISSN:
0721-7714
Subject:
Mangifera indica; cotyledons; cryopreservation; cultivars; explants; genetic improvement; germplasm conservation; mangoes; nitrogen; somatic embryogenesis; vitrification
Abstract:
... In this paper, we described the direct somatic embryogenesis from both immature cotyledon cuts and nucelli in the same mango cultivar (Mangifera indica L. var Zihua), studied the effect of growth conditions of embryogenic cultures (EMs) on cryopreservation and compared the cryopreservation response of EMs induced from these two different explants. Histological studies demonstrated that EMs derived ...
DOI:
10.1007/s00299-006-0232-4
PubMed:
16972094

99. Influence of Pre-freeze Treatment and Cryo-storageTemperature on the Post-thaw Stability of Canine Red Blood Cells Cryopreserved in the Presence of Hydroxyethyl Starch

Author:
Kim, H.; Itamoto, K.; Tanaka, S.; Nakaichi, M.; Sumida, S.; Taura, Y.
Source:
Veterinary research communications 2007 v.31 no.5 pp. 539-543
ISSN:
0165-7380
Subject:
osmosis; frozen storage; morphometry; cryopreservation; nitrogen; dogs; starch; storage temperature; erythrocytes; cryoprotectants; freeze-thaw cycles; pretreatment; veterinary medicine; thawing; dimensional stability
DOI:
10.1007/s11259-007-3567-6
PubMed:
17265096

100. Influence of cryopreservation on mitochondrial functions in equine spermatozoa

Author:
Schober, D.; Aurich, C.; Nohl, H.; Gille, L.
Source:
Theriogenology 2007 v.68 no.5 pp. 745-754
ISSN:
0093-691X
Subject:
semen; cryopreservation; energy metabolism; oxygen consumption; sperm motility; cell respiration; cytochrome-c oxidase; mitochondria; oxidation; spermatozoa; stallions; membrane potential
Abstract:
... Cryopreservation of spermatozoa is of essential importance for artificial insemination and breeding programs in horses. Besides other factors, spermatozoal motility depends on mitochondrial energy metabolism. Based on changes of single mitochondrial functions it has been suggested that mitochondrial damage during cryopreservation could be a major reason for diminished post thaw semen quality. Howe ...
DOI:
10.1016/j.theriogenology.2007.06.004