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... Soil deficiencies of zinc (Zn) and boron (B) limit crop production in Nepal. Improving the micronutrient status of plants would increase yield and increase micronutrient content of the seeds, leading to better nutrition of the progeny crop and to improved human micronutrient nutrition. The primary micronutrient problem in grain legumes is B deficiency, while in rice (Oryza sativa), Zn deficiency i ...
... An in vivo shikimate accumulation assay with excised leaf tissue was developed to provide a fast and reliable method for identifying glyphosate-resistant plants. The assay is based on glyphosate-induced accumulation of shikimate. There was a linear accumulation of shikimate in excised leaf discs of soybean and canola treated with 250 μμM glyphosate for 48 h. The IC₅₀ for the accumulation of shikim ...
... A rapid sensitive microplate assay for the determination of peroxidase activity in wheat, flour, or individual wheat kernels has been developed using a commercially prepared 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) solution. The assay derives a six-point calibration curve based on commercially available horseradish peroxidase (r2 > 0.990), while simultaneously a ...
... Previous studies on rare earth element (REE) bioaccumulation have focused on their accumulation rate and fractionation, but the processes involved remain unclear. In this study, the accumulation and fractionation of REEs in wheat (Triticum aestivum L.) were investigated using solution culture with exogenous mixed REEs. A decrease in REE contents was observed from the roots to the tops of wheat. Si ...
... Glyphosate is used extensively throughout the world as a burndown and in-crop herbicide. A spectrophotometric assay was evaluated as an inexpensive and rapid procedure to measure shikimate accumulation in conventional corn and soybean following glyphosate application. Shikimate levels peaked between 4 and 7 days after application in both corn and soybean. It is useful to understand the behavior of ...
... Cyclic hydroxamic acids are secondary metabolites found in the family Poaceae and have been implicated in the allelopathy of rye (Secale cereale). The toxicity of these compounds against plant-parasitic nematodes is unknown. DIBOA (2,4-dihydroxy-(2H)-1,4-benzoxazin-3(4H)- one), DIMBOA (2,4-hydroxy-7-methoxy-(2H)-1,4-benzoxazin-3(4H)- one), and their degradation products BOA (benzoxazolin-2(3H)-one ...
carbon dioxide; biosynthesis; Datura stramonium; nicotine; scopolamine; air temperature; elevated atmospheric gases; global warming; Nicotiana tabacum; atropine; chemical constituents of plants; drug crops
Abstract:
... The influence of recent and projected changes in atmospheric carbon dioxide concentration [CO2] with and without concurrent increases in air temperature was determined with respect to growth characteristics and production of secondary compounds (alkaloids) in tobacco (Nicotiana tabacum L.) and jimson weed (Datura stramonium L.) over a ca. 50-day period. Rising [CO2] above that present at the begin ...
... Seedlings of two cultivars of sorghum (Sorghum bicolor) differing in aluminum (Al) tolerance grown in a pH 4.0 Clark's nutrient solution were exposed to Al at concentrations of 0 and 185 micromolar for 7 days. The organic acid concentrations in the roots and leaves and in the external solution were determined, as well as the activities of the enzymes related to the biosynthesis and degradation of ...
Citrus reshni; mandarins; seedlings; leaves; aluminum; phytotoxicity; stress tolerance; photosynthesis; net assimilation rate; adenosine diphosphate; glucose; phosphorylase; ribulose-bisphosphate carboxylase; sucrose-phosphate synthase; enzyme activity; chemical constituents of plants; chlorophyll; quantitative analysis; protein content
Abstract:
... 'Cleopatra' tangerine (Citrus reshni Hort. ex Tanaka) seedlings were irrigated daily for 8 weeks with 1/4 strength Hoagland's nutrient solution containing 0 (control) or 2 mM aluminum (Al). Leaves from Al-treated plants had decreased CO2 assimilation and stomatal conductance, but increased intercellular CO2 concentrations compared with control leaves. On a leaf area basis, 2 mM Al increased activi ...
calcium chloride; plant growth; application timing; salinity; proline; cations; ion transport; crop yield; leaves; sodium chloride; potassium; Senna alexandrina; chemical constituents of plants; sodium; dry matter partitioning; roots; salt stress; shoots
Abstract:
... The effect of NaCl and CaCl2 on growth parameters, ionic relations, and proline level in senna (Cassia angustifolia) plant was investigated in a pot culture experiment using NaCl (80 mM and 160 mM), CaCl2 (5 mM and 10 mM), and the combined salt of NaCl + CaCl2 (80 mM + 10 mM and 160 mM + 10 mM). These treatments were given to the growing plant separately at pre-flowering (A1), flowering (A2) and p ...
anthraquinones; Morinda citrifolia; medicinal plants; spectral analysis; chemical constituents of plants; NADH or NADPH oxidoreductases; chemical structure
ultraviolet-visible spectroscopy; chemical analysis; plant extracts; high performance liquid chromatography; Lepidium meyenii; secondary metabolites; spectral analysis; chemical constituents of plants; mass spectrometry; chemical structure; Peru
Abstract:
... The macamides are a distinct class of secondary metabolites that have so far been found only in Lepidium meyenii Walp. (Maca). Using HPLC-UV-MS/MS, the main macamides have been identified as n-benzylhexadecanamide, n-benzyl-(9Z)-octadecenamide, n-benzyl-(9Z, 12Z)-octadecadienamide, n-benzyl-(9Z, 12Z, 15Z)-octadecatrienamide and n-benzyloctadecanamide. The identities of n-benzyl-(9Z)-octadecenamide ...
Nicotiana tabacum; tobacco; leaves; chemical constituents of plants; imidacloprid; quantitative analysis; insecticide residues; high performance liquid chromatography
Abstract:
... A practical method for analysis of residues of imidacloprid in baked tobacco leaves has been developed using HPLC with liquid-liquid partition clean-up. Imidacloprid was extracted with ethyl acetate under ultrasound, and cleaned up by liquid-liquid partition with 50 g litre(-1) aqueous sodium chloride followed by dichloromethane. The water-soluble and fat-soluble components in tobacco, which inter ...
parasitoids; plant-insect relations; chemical constituents of plants; volatile compounds; induced resistance; electrophysiology; host plants; insect control
Abstract:
... Many parasitic wasps are attracted to volatiles that are released by plants when attacked by potential hosts. The attractiveness of these semiochemicals from damaged plants has been demonstrated in many tritrophic systems, but the physiological mechanisms underlying the insect responses are poorly understood. We recorded the antennal perception by three parasitoids (Cotesia marginiventris, Micropl ...
Tilia cordata; bioassays; bark; pelargonic acid; plant extracts; insect control; antifeedants; insect pests; Hylobius abietis; plant-insect relations; chemical constituents of plants; chemical composition
Abstract:
... Linden (Tilia cordata) bark was shown to contain an antifeedant effective against the large pine weevil, Hylobius abietis. Soxhlet extraction of inner and outer bark resulted in an extract that showed antifeedant activity in a microfeeding assay. The extract was fractionated by chromatography on silica gel using gradient elution with solvents of increasing polarity. The content of the fractions ob ...
plant pathogenic bacteria; antibacterial properties; Micrococcus luteus; stems; Salmonella Enteritidis; Botrytis cinerea; disease control; plant pathogenic fungi; Bacillus subtilis; chemical constituents of plants; bark; Khaya ivorensis; limonoids; antifungal properties; Republic of the Congo
Abstract:
... Chemical investigation of the diethyl ether extract of the stem bark of Khaya ivorensis A Chev (Meliaceae) afforded ten limonoids of angolensates, ring D-opened limonoids and mexicanolides. The structures of the limonoids isolated were determined by comparison of their 1H and 13C NMR data with those reported in the literature. These compounds were evaluated for their antifungal activity against th ...
mosses and liverworts; phenolic compounds; esters; chemical constituents of plants; antifungal properties; Cladosporium herbarum; plant pathogenic fungi; antifeedants; antifeeding activity; Spodoptera littoralis; Chile
Abstract:
... A chemical study of a dichloromethane extract of Balantiopsis cancellata led to the isolation of four known 2-phenylethanol esters (1-4) and a phenylethanediol benzoate (5). Antifeedant activity toward Spodoptera littoralis (disk-choice bioassay) and growth inhibition of the phytopathogen Cladosporium herbarum in TLC-bioautography assays were determined. The results show that the antifeedant and a ...
Triticum aestivum; wheat; fungal diseases of plants; Thanatephorus cucumeris; Glomerella cingulata; plant pathogenic fungi; disease control; fungicides; plant extracts; roots; chemical constituents of plants; Celastrus; antifungal properties; China
Abstract:
... Pristimerin and celastrol isolated from the roots of Celastrus hypoleucus (Oliv) Warb f argutior Loes exhibited inhibitory effects against diverse phytopathogenic fungi. Pristimerin and celastrol were found to inhibit the mycelial growth of Rhizoctonia solani Kuhn and Glomerella cingulata (Stonem) Spauld & Schrenk in vitro by 83.6 and 62.6%, respectively, at 10 microgram ml(-1). Pristimerin showed ...
anthocyanins; functional foods; free radicals; chlorogenic acid; Solanum tuberosum; potatoes; carotenoids; chemical constituents of plants; cooking; antioxidants; phenolic compounds
Abstract:
... The content of compounds in potato that may act as antioxidants in the human diet is not widely appreciated. Carotenoids are present in the flesh of all potatoes. The contents mentioned in the literature range from 50 to 100 micrograms per 100 g fresh weight (FW) in white-fleshed varieties to 2000 micrograms per 100 g FW in deeply yellow to orange-fleshed cultivars. The carotenoids in potato are p ...
chemical composition; volatile compounds; forest trees; wood; forest management; electronic nose; polymers; chemical constituents of plants; plant taxonomy; chemotaxonomy; forest ecology; adsorption
Abstract:
... An electronic aroma detection (EAD) technology known as conductive polymer analysis (CPA) was evaluated as a means of identifying and discriminating woody samples of angiosperms and gymnosperms using an analytical instrument (electronic nose) that characterizes the aroma profiles of volatiles released from excised wood into sampled headspace. The instrument measures electrical-resistance changes g ...
Glycine max; soybeans; pest resistance; insect pests; chemical constituents of plants; plant-insect relations; flavonoids; insect physiology
Abstract:
... The identification and quantification of flavonoids (rutin and genistin) present in extracts of soybean genotypes, and their effects on the biology and physiology of Anticarsia gemmatalis Hubner (Lep.: Noctuidae) were studied. Analysis of covariance and bicoordinate utilization plots were used to remove the effect of feeding time from pupal weight and consumption as well as to separate pre- and po ...
2,2-diphenyl-1-picrylhydrazyl; antioxidants; chemical constituents of plants; plant tissues; polyphenols; red wines
Abstract:
... Polyphenolic phytochemicals present in certain plant tissues exhibit multifunctional properties, in terms of expressing in vitroantioxidant activity. In the case of wines, which can be considered as very complex mixtures of polyphenols, a trend has been revealed by previous studies regarding the correlation of values from antioxidant tests based on different chemical backgrounds. In this respect, ...
... Biculture legume–cereal cover cropping may enhance above- and belowground biomass yields and C and N contents. The increase in C and N supply to the soil has the potential to improve soil quality and crop productivity compared with monoculture cover crop species. We examined above- and belowground (0- to 120-cm soil depth) biomass yields and C and N contents of a legume [hairy vetch (Vicia villosa ...
soil insects; insect pests; insect control; soil fumigation; plant extracts; Brassica juncea; Brassica napus var. napus; shoots; seeds; roots; chemical constituents of plants; isothiocyanates; glucosinolates; application rate; mechanism of action; soil temperature
Abstract:
... Four pure isothiocyanates (methyl, 2-propenyl, benzyl and 2-phenylethyl isothiocyanate), hydrolysing tissue of two brassicas rich in either 2-propenyl or 2-phenylethyl isothiocyanate, and the methyl isothiocyanate-generating pesticide metam-sodium were tested in vapour exposure tests for biological activity against a model soil insect both in vitro and in the presence of three contrasting soils an ...
... BACKGROUND AND AIMS: Both nutrient availability and defoliation affect the carbon-nutrient balance in plants, which in turn influences biomass allocation (e.g. shoot-to-root ratio) and leaf chemical composition (concentration of nitrogen and secondary compounds). In this study it is questioned whether defoliation alters biomass allocation and chemical defence in a similar fashion to the response t ...
Olea europaea; olives; leaves; trichomes; boron; metabolism; nutrient deficiencies; stress tolerance; chemical constituents of plants; phenolic compounds; flavonoids; quantitative analysis; field experimentation; growth chambers
Abstract:
... Boron deficiency is the most frequent micronutrient disorder in olive (Olea spp.) orchards. We tested the hypothesis that plant boron status affects phenolic metabolism, which, in turn, influences several ecophysiological traits of olive (Olea europaea L.) trees, by studying the effects of boron deficiency on leaf phenolic compounds of olive in a growth chamber experiment (CE) and a field experime ...
Olea europaea; olives; leaves; phloem; boron; nutrient deficiencies; stress tolerance; mannitol; biosynthesis; photosynthesis; net assimilation rate; nutrient transport; source-sink relationships; tree growth; chemical constituents of plants; sucrose; glucose; myo-inositol; fructose; quantitative analysis; Greece
Abstract:
... For plant species in which a considerable portion of the photoassimilates are translocated in the phloem as sugar alcohols, boron is freely translocated from mature organs to growing tissues. However, the effects of decreased plant boron status on boron remobilization are poorly understood. We conducted a growth chamber experiment (CE) and a field experiment (FE) to study the effects of low boron ...
germplasm; plant breeding; chemical constituents of plants; guayule; Parthenium argentatum; artificial selection; apomixis; new crops; plant genetic resources; latex; genotype; genetic variation; commercialization; plant morphology; domestication; yield components; genetic markers
Abstract:
... Breeding a new domestic crop, such as guayule, is not appreciably different from enhancement and breeding of conventional crops. In both instances, plant breeders take the available germplasm and search for genetic variability in the desired traits. The major differences are that in new crops plant breeders are often working with an unfamiliar species that is not yet fully domesticated and the ava ...