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1. Infectious bronchitis virus replication in the chicken embryo related cell line

Author:
Ferreira, Helena L.; Pilz, Daniela; Mesquita, Ligia G.; Cardoso, Tereza
Source:
Avian pathology 2003 v.32 no.4 pp. 413-417
ISSN:
0307-9457
Subject:
Infectious bronchitis virus; RNA; cell fusion; chicken eggs; chickens; cytopathogenicity; electron microscopy; fluorescence; fluorescent antibody technique; kidney cells; reverse transcriptase polymerase chain reaction; titration; virus replication; viruses
Abstract:
... The susceptibility of the chicken embryo related (CER) cell line to infectious bronchitis virus (IBV M41) was characterized after five consecutive passages in CER cells. Virus replication was monitored by cytopathic effect observation, electron microscopy, indirect immunofluorescence, and reverse transcription polymerase chain reaction (RT-PCR). At 96 h post-infection (p.i.), the cytopathic effect ...
DOI:
10.1080/0307945031000121167