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... Twenty-four strains of Salmonella enteritidis, isolated from several outbreaks of salmonellosis from different poultry farms in India, were checked for the plasmid profile and detection of virulence gene(s) by PCR. Most of the strains contained only a single plasmid of 55 kb. Additional plasmids of 23.2 kb and 8.7 kb were seen in one of the strains, and another strain carried only two plasmids of ...
... The quail is a valuable farm and laboratory animal. Yet molecular information about this species remains scarce. We present here the first genetic linkage map of the Japanese quail. This comprehensive map is based solely on amplified fragment length polymorphism (AFLP) markers. These markers were developed and genotyped in an F2 progeny from a cross between two lines of quail differing in stress r ...
... The INK4b-ARF-INK4a locus on human chromosome 9p21 (Human Genome Organization designation CDKN2B-CDKN2A), and the corresponding locus on mouse chromosome 4, encodes three distinct products: two members of the INK4 cyclin-dependent kinase inhibitor family and a completely unrelated protein, ARF, whose carboxyl-terminal half is specified by the second exon of INK4a but in an alternative reading fram ...
chickens; restriction fragment length polymorphism; alleles; haplotypes; feed conversion; body weight; energy metabolism; gene interaction; eggs; age at first egg; gluconeogenesis; egg weight; gene frequency; feed intake; phosphoenolpyruvate carboxykinase (GTP); specific gravity; isozymes; animal genetics; quantitative traits
Abstract:
... White Leghorn chickens from a nonselected closed population were typed for two RFLP located in the 3' end of the gene coding for cytosolic phosphoenolpyruvate carboxykinase (PEPCK-C), a major control gene of gluconeogenesis. The two RFLP gave rise to three alleles (or haplotype classes), thus defining six genotypes. Feed efficiency (FE) and residual feed consumption (RFC) varied significantly amon ...
... The amino acid sequence of satyr tragopan lysozyme and its activity was analyzed. Carboxymethylated lysozyme was digested with trypsin and the resulting peptides were sequenced. The established amino acid sequence had three amino acid substitutions at positions 103 (Asn to Ser), 106 (Ser to Asn), and 121 (His to Gln) comparing with Temminck's tragopan lysozyme and five amino acid substitutions at ...
... For Apicomplexa (members) the host cell invasion is realized with the help of the organelles located at the apical tip of parasites. In this research paper the characterization of five chicken monoclonal antibodies (mabs) produced against Eimeria acervulina sporozoites is described. All mabs reacted with molecules belonging to the apical complex of chicken Eimeria sporozoites. On immunofluorescenc ...
chickens; coccidiosis; live vaccines; Eimeria; lesions (animal)
Abstract:
... Male and female floor-reared chickens were immunized with a live, attenuated anticoccidial vaccine (Paracox) and then, 28 days after vaccination, were challenged with virulent strains of Eimeria acervulina, Eimeria brunetti, Eimeria maxima, Eimeria necatrix or Eimeria tenella. The relationship between the post-challenge growth rate and specific gross lesion grades, matched in individual birds, was ...
... Lymphocyte proliferation and interleukin (IL)-2 and IL-6 levels in serum were measured as indicators of cell-mediated immunity after immunization of chickens with a commercial killed Salmonella enteritidis (SE) vaccine or experimental subunit vaccines of crude protein (CP) extract or the outer membrane protein (OMP). Significantly increased proliferative responses to SE flagella, but not lipopolys ...
chickens; serotypes; drug evaluation; live vaccines; infectious bronchitis; strain differences; Infectious bronchitis virus; vaccination; vaccine development
Abstract:
... In 1998, novel strains of infectious bronchitis virus (IBV) were identified in chickens from the southeastern United States and classified as a new serotype designated Georgia 98 (GA98). Because of the widespread nature of the GA98 virus in the southeastern United States and the lack of adequate protection with the DE072 vaccine, we developed a specific vaccine for the GA98 serotype. The GA98/0470 ...
Jossi Hillel; Martien A.M. Groenen; Michèle Tixier-Boichard; Abraham B. Korol; Lior David; Valery M. Kirzhner; Terry Burke; Asili Barre-Dirie; Richard P.M.A. Crooijmans; Kari Elo; Marcus W. Feldman; Paul J. Freidlin; Asko Mäki-Tanila; Marian Oortwijn; Pippa Thomson; Alain Vignal; Klaus Wimmers; Steffen Weigend
... In a project on the biodiversity of chickens funded by the European Commission (EC), eight laboratories collaborated to assess the genetic variation within and between 52 populations from a wide range of chicken types. Twenty-two di-nucleotide microsatellite markers were used to genotype DNA pools of 50 birds from each population. The polymorphism measures for the average, the least polymorphic po ...
Pasteurella multocida; chickens; ferritin; protein content; transmission electron microscopy; virulence; virulent strains
Abstract:
... Capsule thickness of avian Pasteurella multocida type A strains was determined by transmission electron microscopy after labeling with polycationic ferritin and compared with their pathogenicity for chickens. The capsule thickness of P. multocida strains Pm-18 and X-73 was 81.4 and 50.1 nm on average, respectively. These strains were highly virulent for chicken, whereas the less virulent strains P ...
... Two cases of Mycoplasma gallisepticum infection in different avian species in backyard gamebird operations in Slovenia were investigated. In the first case, M gallisepticum was associated with severe respiratory disease with almost 20 per cent mortality in pheasants, whereas the infection was less pathogenic for chickens and turkeys reared at the same site. The M gallisepticum isolates from pheasa ...
... The reverse transcriptase-polymerase chain reaction/restriction fragment length polymorphism (RT-PCR/RFLP) technique was used for identification and characterization of Egyptian field strains of infectious bursal disease viruses (IBDVs) that caused severe outbreaks with (30%-60%) mortality rate. Twenty-four bursal samples collected from 24 field outbreaks in commercially reared chicken flocks expe ...
... We used slot blot hybridization of the hypervariable regions of the S1 subunit of spike peplomer gene to identify and characterize infectious bronchitis virus (IBV) strains. Template DNA was created from six reference strain IBVs of different serotypes and immobilized on a nitrocellulose membrane. We synthesized digoxigenin-labeled probes from reference and unknown field viruses and hybridized the ...
... The prevalence of Toxoplasma gondii in free range chickens is a good indicator of the prevalence of T. gondii oocysts in the environment because chickens feed from the ground. In the present study, prevalence of T. gondii in 40 free range chickens (Gallus domesticus) from a rural area surrounding Paraná, Brazil was assessed. Blood, heart, and brain from each chicken were examined for T. gondii in ...
... An avian pathogenic Escherichia coli (APEC) strain designated SHS4, isolated from a chicken with clinical signs of swollen head syndrome (SHS), adhered to but did not invade Hep-2 and tracheal epithelial cells. The PCR amplified fimA, csgA and tsh gene sequences. It produced Ia, Ib, E1, E3, K, and B colicins, but not colicin V and aerobactin. It harboured two plasmids of 60 and 98 MDa and was resi ...