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You searched for: Publication Year 2002 Remove constraint Publication Year: 2002 Subject Western blotting Remove constraint Subject: Western blotting
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1. Altering the surface properties of baculovirus Autographa californica NPV by insertional mutagenesis of the envelope protein gp64

Author:
Alexandra Spenger; Reingard Grabherr; Lars Töllner; Hermann Katinger; Wolfgang Ernst
Source:
European journal of biochemistry 2002 v.269 no.18 pp. 4458-4467
ISSN:
0014-2956
Subject:
Autographa californica; Autographa californica multiple nucleopolyhedrovirus; Human immunodeficiency virus 1; Western blotting; antigens; binding properties; enzyme-linked immunosorbent assay; genetic engineering; glycoproteins; host range; humans; insects; insertional mutagenesis; membrane fusion; molecular biology; mutants; peptides; probability; receptors; viral envelope proteins; viruses
Abstract:
... The envelope protein gp64 of the baculovirus Autographa californica nuclear polyhedrosis virus is essential for viral entry into insect cells, as the glycoprotein both mediates pH‐dependent membrane fusion and binds to host cell receptors. Surface modification of baculovirus particles by genetic engineering of gp64 has been demonstrated by various strategies and thus has become an important and ...
DOI:
10.1046/j.1432-1033.2002.03135.x

2. Assessment of the floral origin of honey by SDS-page immunoblot techniques

Author:
Baroni, M.V.; Chiabrando, G.A.; Costa, C.; Wunderlin, D.A.
Source:
Journal of agricultural and food chemistry 2002 v.50 no.6 pp. 1362-1367
ISSN:
0021-8561
Subject:
cross reaction; Helianthus annuus; Eucalyptus; biomarkers; polyacrylamide gel electrophoresis; Western blotting; pollen; honey
Abstract:
... We report on the development of a novel alternative method for the assessment of floral origin in honey samples based on the study of honey proteins using immunoblot assays. The main goal of our work was to evaluate the use of honey proteins as chemical markers of the floral origin of honey. Considering that honeybee proteins should be common to all types of honey, we decided to verify the usefuln ...
DOI:
10.1021/jf011214i
PubMed:
11879003

3. Brassica napus soluble epoxide hydrolase (BNSEH1): Cloning and characterization of the recombinant enzyme expressed in Pichia pastoris

Author:
Stefan Bellevik; Jiaming Zhang; Johan Meijer
Source:
European journal of biochemistry 2002 v.269 no.21 pp. 5295-5302
ISSN:
0014-2956
Subject:
fatty acids; Komagataella pastoris; polyacrylamide gel electrophoresis; Western blotting; gel chromatography; Brassica napus; antibodies; recombinant proteins; rapid amplification of cDNA ends; amino acids; molecular weight; screening; leaves; Arabidopsis thaliana; complementary DNA; yeasts; seed oils; methyl jasmonate; epoxide hydrolase; cDNA libraries; fatty acid metabolism; hydrolysis; temperature; metabolic engineering; epoxides; pH
Abstract:
... Epoxide hydrolase (EC 3.3.2.3) in plants is involved in the metabolism of epoxy fatty acids and in mediating defence responses. We report the cloning of a full‐length epoxide hydrolase cDNA (BNSEH1) from oilseed rape (Brassica napus) obtained by screening of a cDNA library prepared from methyl jasmonate induced leaf tissue, and the 5′‐RACE technique. The cDNA encodes a soluble protein contai ...
DOI:
10.1046/j.1432-1033.2002.03247.x

4. The Carbonyl Content of Specific Plasma Proteins Is Decreased by Dietary Copper Deficiency in Rats

Author:
Cockell, Kevin A.; Belonje, Bartholomeus
Source:
Journal of nutrition 2002 v.132 no.9 pp. 2514-2518
ISSN:
0022-3166
Subject:
Western blotting; animal models; biomarkers; blood proteins; copper; derivatization; diet; erythrocytes; heart; lipoproteins; liver; oxidation; oxidative stress; polyacrylamide gel electrophoresis; rats; weanlings
Abstract:
... Copper (Cu) deficiency is associated with increased susceptibility of tissue homogenates or lipoproteins to oxidation in vitro. Plasma is easily sampled and contains both lipid and protein components that may be susceptible to oxidation, making it appropriate to investigate plasma oxidation variables as biomarkers of in vivo oxidative stress. Oxidation of plasma proteins may be discernible as an i ...
PubMed:
12221202

5. Cellular Distribution of Anionic Antimicrobial Peptide in Normal Lung and during Acute Pulmonary Inflammation

Author:
A. J. Fales-Williams; K. A. Brogden; E. Huffman; J. M. Gallup; M. R. Ackermann
Source:
Veterinary pathology v.39 no.6 pp. 706-711
ISSN:
1544-2217
Subject:
Mannheimia; Pasteurella; Pseudomonas aeruginosa; Western blotting; adults; animal pathology; antibodies; antimicrobial peptides; bacteria; bronchioles; calves; cows; humans; immunohistochemistry; inflammation; inoculum; lipopolysaccharides; respiratory tract diseases; sheep
Abstract:
... Anionic peptides (APs) are small antimicrobial peptides present in human and ovine lung. In this study APs were also detected in bovine lung, and production of APs in lungs with acute inflammation induced by various stimuli was determined. The distribution and intensity of APs were determined by immunohistochemistry in lungs of 1) neonatal calves (1-3 days of age) inoculated with Mannheimia (Paste ...
DOI:
10.1354/vp.39-6-706

6. Characterisation of antisera to recombinant IgA of the common brushtail possum (Trichosurus vulpecula)

Author:
Rawson, Renee R.; Belov, Katherine; Gidley-Baird, A. Angus; Cooper, Desmond W.
Source:
Veterinary immunology and immunopathology 2002 v.88 no.1-2 pp. 89-95
ISSN:
0165-2427
Subject:
Macropus eugenii; Macropus giganteus; Phascolarctos cinereus; Trichosurus vulpecula; Western blotting; antiserum; blood serum; cross reaction; enzyme-linked immunosorbent assay; immunoglobulin A; immunoglobulin G; mucosal immunity; possums; recombinant proteins
Abstract:
... One of the limiting factors in understanding immune responses in marsupials is the scarcity of marsupial specific immunological reagents. This paper describes the characterisation of an antiserum raised against a recombinant protein of the constant region of the heavy chain of IgA (Cα) of the common brushtail possum (Trichosurus vulpecula). The availability of a marsupial specific anti-IgA provide ...
DOI:
10.1016/S0165-2427(02)00126-5

7. Characterisation of the first Australian isolate of Neospora caninum from cattle

Author:
Miller, C.M.D.; Quinn, H.E.; Windsor, P.A.; Ellis, J.T.
Source:
Australian veterinary journal 2002 v.80 no.10 pp. 620-625
ISSN:
0005-0423
Subject:
Holstein; Neospora caninum; Toxoplasma gondii; Western blotting; antigens; calves; cerebrum; coasts; containers; dairy herds; electron microscopy; encephalitis; histopathology; mice; nucleotide sequences; parasites; pregnancy; pups; sequence analysis; serology; seroprevalence; spinal cord; tissue culture; transplacental transmission; vaccine development; virulence; Australia
Abstract:
... Objective To isolate Neospora caninum from a congenitally infected calf. Procedure A calf was obtained from a N caninum infected dam maintained in a dairy herd of Holstein-Friesian cattle located on the south coast of NSW near Nowra. The calf was euthanased and samples collected for serology and pathology. Samples of brain and spinal cord of the calf were homogenised and injected into immunocompro ...
DOI:
10.1111/j.1751-0813.2002.tb10967.x

8. Characterization of the lectin from females of Phlebotomus duboscqi sand flies

Author:
Petr Volf; Sona Skarupová; Petr Man
Source:
European journal of biochemistry 2002 v.269 no.24 pp. 6294-6301
ISSN:
0014-2956
Subject:
Leishmania; Lutzomyia longipalpis; Phlebotomus duboscqi; Phlebotomus papatasi; Western blotting; antibodies; cDNA libraries; carbohydrate binding; complementary DNA; epithelial cells; females; fluorescent antibody technique; gel chromatography; hemagglutination; immunoaffinity chromatography; immunoglobulins; lectins; matrix-assisted laser desorption-ionization mass spectrometry; midgut; molecular weight; polyacrylamide gel electrophoresis; salivary glands; silver nitrate
Abstract:
... Lectin from females of the important sand fly vector, Phlebotomus duboscqi (Diptera: Psychodidae), was isolated by immunoaffinity chromatography using a minicolumn with immobilized anti‐lectin immunoglobulins. Carbohydrate‐binding specificity of active fractions corresponded to that of midgut and salivary gland lysates. Haemagglutination was inhibited by d‐glucosamine, d‐galactosamine and d‐mannos ...
DOI:
10.1046/j.1432-1033.2002.03349.x

9. Chitinolytic activities in Bacillus thuringiensis and their synergistic effects on larvicidal activity

Author:
Liu, M.; Cai, Q.X.; Liu, H.Z.; Zhang, B.H.; Yan, J.P.; Yuan, Z.M.
Source:
Journal of applied microbiology 2002 v.93 no.3 pp. 374-379
ISSN:
1364-5072
Subject:
Bacillus thuringiensis; Spodoptera exigua; Western blotting; bioassays; biopesticides; chitinase; colorimetry; insecticidal properties; larvae; molecular weight; pH; polyacrylamide gel electrophoresis; serotypes; synergism; toxicity
Abstract:
... Aims: To investigate the distribution of chitinase in Bacillus thuringiensis strains, and the enhancing effects of the chitinase-producing B. thuringiensis strains on insecticidal toxicity of active B. thuringiensis strain against Spodoptera exigua larvae. Methods and Results: The chitinolytic activities of B. thuringiensis strains representing the 70 serotypes were investigated by the whitish opa ...
DOI:
10.1046/j.1365-2672.2002.01693.x
PubMed:
12174034

10. Cloning, expression, purification, and biological activity of five feline type I interferons

Author:
Wonderling, Ramani; Powell, Tim; Baldwin, Susan; Morales,Tony; Snyder, Scott; Keiser, Kathy; Hunter, Shirley; Best, Elaine; McDermott, Martin J.; Milhausen, Michael
Source:
Veterinary immunology and immunopathology 2002 v.89 no.1-2 pp. 13-27
ISSN:
0165-2427
Subject:
sequence analysis; interferon-alpha; antiviral properties; Komagataella pastoris; complementary DNA; cats; cell culture; glycosylation; humans; recombinant proteins; yeasts; Western blotting; amino acid sequences; amino acids; clones; genes; viruses; immune response
Abstract:
... Type I interferons (IFN) are important mediators of the host defense against viral infections in mammals. In humans multiple subtypes of IFN-α exist, most of which possess antiviral activity. Little is known about the type I IFN genes in cats and the role they may play in feline immunological responses to viruses. We have isolated cDNAs encoding five feline IFN-α (feIFN) subtypes that share from 9 ...
DOI:
10.1016/S0165-2427(02)00188-5

11. Comparison of a Putative Second Serotype of Chicken Infectious Anemia Virus with a Prototypical Isolate II. Antigenic and Physicochemical Characteristics

Author:
Spackman, E.; Cloud, S.S.; Rosenberger, J.K.
Source:
Avian diseases 2002 v.46 no.4 pp. 956-963
ISSN:
0005-2086
Subject:
Chicken anemia virus; DNA primers; Southern blotting; Western blotting; bird diseases; broiler breeders; chloroform; enzyme-linked immunosorbent assay; fluorescent antibody technique; neutralization tests; nucleotide sequences; pH; polymerase chain reaction; pullets; serotypes; thermal stability; viruses
Abstract:
... A putative new serotype of chicken infectious anemia virus (CIAV) isolated from 17-wk-old broiler breeder pullets was compared with a known, previously characterized CIAV isolate, the Del-Ros strain. Physicochemical characteristics evaluated included thermal stability, size, pH, and chloroform sensitivity. Physicochemically, CIAV-7 was identical to CIAV. The virus isolates were compared antigenica ...
DOI:
10.1637/0005-2086(2002)046[0956:COAPSS]2.0.CO;2

12. Concerted regulation of free arachidonic acid and hormone‐induced steroid synthesis by acyl‐CoA thioesterases and acyl‐CoA synthetases in adrenal cells

Author:
Paula Maloberti; Rocío Castilla Lozano; Pablo G. Mele; Florencia Cano; Cecilia Colonna; Carlos F. Mendez; Cristina Paz; Ernesto J. Podestá
Source:
European journal of biochemistry 2002 v.269 no.22 pp. 5599-5607
ISSN:
0014-2956
Subject:
Western blotting; acyl coenzyme A; antibodies; arachidonic acid; corticotropin; immunocytochemistry; immunohistochemistry; inhibitory concentration 50; long-chain-fatty-acid-CoA ligase; neoplasm cells; nordihydroguaiaretic acid; steroidogenesis; synergism
Abstract:
... Although the role of arachidonic acid (AA) in the regulation of steroidogenesis is well documented, the mechanism for AA release is not clear. Therefore, the aim of this study was to characterize the role of an acyl‐CoA thioesterase (ARTISt) and an acyl‐CoA synthetase as members of an alternative pathway in the regulation of the intracellular levels of AA in steroidogenesis. Purified recombina ...
DOI:
10.1046/j.1432-1033.2002.03267.x

13. Construction and Immunogenicity Studies of Recombinant Fowl Poxvirus Containing the S1 Gene of Massachusetts 41 Strain of Infectious Bronchitis Virus

Author:
Wang, Xiuqing; Schnitzlein, William M.; Tripathy, Deoki N.; Girshick, Theodore; Khan, Mazhar I.
Source:
Avian diseases 2002 v.46 no.4 pp. 831-838
ISSN:
0005-2086
Subject:
Infectious bronchitis virus; Western blotting; blood serum; chickens; complementary DNA; fluorescent antibody technique; fowl pox; genes; glycoproteins; humoral immunity; immune response; immunization; immunoglobulin G; infectious bronchitis; neutralization; neutralizing antibodies; staining; vaccines; virulence; virulent strains; viruses; Massachusetts
Abstract:
... The spike 1 (S1) surface glycoprotein of infectious bronchitis virus (IBV) is the major inducer of the generation of virus neutralizing antibodies, and the administration of purified S1 has been shown to elicit a protective immune response against virulent virus challenge. On the basis of these observations, recombinant fowl poxvirus (rFPV) containing a cDNA copy of the S1 gene of IBV Mass 41 (rFP ...
DOI:
10.1637/0005-2086(2002)046[0831:CAISOR]2.0.CO;2

14. Cross-sectional study of serum antibodies against Sarcocystis neurona in cats tested for antibodies against Toxoplasma gondii

Author:
Rossano, M.G.; Murphy, A.J.; Vrable, R.A.; Vanzo, N.E.; Lewis, S.K.; Sheline, K.D.; Kaneene, J.B.; Mansfield, L.S.
Source:
Journal of the American Veterinary Medical Association 2002 v.221 no.4 pp. 511-514
ISSN:
0003-1488
Subject:
cats; Sarcocystis; seroprevalence; antibodies; fluorescent antibody technique; Toxoplasma gondii; Western blotting; Michigan
DOI:
10.2460/javma.2002.221.511
PubMed:
12184700

15. Detection of antibodies against Sarcocystis neurona in cerebrospinal fluid from clinically normal neonatal foals

Author:
Cook, A.G.; Maxwell, V.B.; Donaldson, L.L.; Parker, N.A.; Ward, D.L.; Morrow, J.K.
Source:
Journal of the American Veterinary Medical Association 2002 v.220 no.2 pp. 208-211
ISSN:
0003-1488
Subject:
foals; Sarcocystis; cerebrospinal fluid; blood serum; antibody detection; protozoal infections; equine protozoal myeloencephalitis; Western blotting
DOI:
10.2460/javma.2002.220.208
PubMed:
12126132

16. Detection of ligands for selectins in the oligosaccharide fraction of human milk

Author:
Silvia Rudloff; Christina Stefan; Gottfried Pohlentz; Clemens Kunz
Source:
European journal of nutrition 2002 v.41 no.2 pp. 85-92
ISSN:
1436-6207
Subject:
Western blotting; breast milk; cell adhesion; epitopes; ethanolamine; immunoassays; milk; monoclonal antibodies; oligosaccharides; spectroscopy; thin layer chromatography
Abstract:
... Background Human milk contains a large variety of oligosaccharides which show structural similarities with ligands for selectins, a family of cell adhesion molecules which are involved in many cell-cell interactions. Aim of the study Due to their structural similarity with selectin ligands, human milk oligosaccharides were labelled with phosphatidyl ethanolamine to be able to investigate specific ...
DOI:
10.1007/s003940200012
PubMed:
12083318

17. Detection of rice grassy stunt tenuivirus nonstructural proteins p2, p5 and p6 from infected rice plants and from viruliferous brown planthoppers

Author:
Chomchan, P.; Miranda, G.J.; Shirako, Y.
Source:
Archives of virology 2002 v.147 no.12 pp. 2291-2300
ISSN:
0304-8608
Subject:
plant tissues; rice; insect pests; disease vectors; Nilaparvata lugens; plant diseases and disorders; cell walls; Oryza sativa; Rice grassy stunt virus; host plants; Western blotting; cytoplasm; nucleocapsid proteins; detection; leaves; homogenization; Philippines
DOI:
10.1007/s00705-002-0893-4
PubMed:
12491098

18. Development and characterization of monoclonal antibodies to chicken interleukin-15

Author:
Min, Wongi; Lillehoj, Hyun S.; Li, Guangxing; Sohn, Eun J.; Miyamoto, Tadashi
Source:
Veterinary immunology and immunopathology 2002 v.88 no.1-2 pp. 49-56
ISSN:
0165-2427
Subject:
T-lymphocytes; Western blotting; cell growth; chickens; enzyme-linked immunosorbent assay; genes; interleukin-15; interleukin-2; monoclonal antibodies; polypeptides
Abstract:
... The chicken IL-15 gene was recently cloned and shown to encode a polypeptide with T cell growth factor activity similar to IL-2. To further characterize the chemical and biological properties of chicken IL-15, we generated a panel of monoclonal antibodies against bacterially expressed protein and characterized their binding specificities. All antibodies were reactive by ELISA with recombinant IL-1 ...
DOI:
10.1016/S0165-2427(02)00123-X

19. Distribution of various peptides in citrus fruits (grapefruit, lemon, and orange)

Author:
Sass-Kiss, A.; Sass, M.
Source:
Journal of agricultural and food chemistry 2002 v.50 no.7 pp. 2117-2120
ISSN:
0021-8561
Subject:
interspecific variation; lemon juice; polyacrylamide gel electrophoresis; peptides; orange juice; adulterated products; Western blotting; grapefruit juice; detection
Abstract:
... Tissue- and species-specific peptides of the grapefruit have been investigated by SDS-PAGE and Western blot. Five peptides from the juice and one peptide from the peel were isolated by preparative gel electrophoresis. Polyclonal antibodies were developed against them in mice. It can be established that 82, 63, and 46 kDa peptides occurred exclusively in the samples prepared from the grapefruit and ...
DOI:
10.1021/jf0111615
PubMed:
11902965

20. Effects of obesity and weight loss on the expression of proteins involved in fatty acid metabolism in human adipose tissue

Author:
Fisher, R.M.; Hoffstedt, J.; Hotamisligil, G.S.; Thörne, A.; Rydén, M.
Source:
International journal of obesity 2002 v.26 no.10 pp. 1379-1385
ISSN:
0307-0565
Subject:
Western blotting; actin; adipocytes; adipose tissue; bariatric surgery; binding proteins; body mass index; fatty acid metabolism; fatty acids; free fatty acids; gender; gene expression regulation; humans; insulin resistance; lipolysis; models; obesity; weight loss
Abstract:
... OBJECTIVE: Disturbances in adipocyte lipolysis in obesity may contribute to elevated circulating non-esterified fatty acid (NEFA) concentrations and insulin resistance. In experimental models, NEFA metabolism is influenced by adipocyte proteins such as adipocyte and keratinocyte lipid binding proteins (aP2/ALBP and mal1/KLBP) and fatty acid translocase (CD36). We investigated the effect of obesity ...
DOI:
10.1038/si.ijo.0802110
PubMed:
12355335

21. Effects of the chestnut inner shell extract on the expression of adhesion molecules, fibronectin and vitronectin, of skin fibroblasts in culture

Author:
Yeon Sook Chi; Moon Young Heo; Ji Hun Chung; Byoung Kee Jo; Hyun Pyo Kim
Source:
Archives of pharmacal research 2002 v.25 no.4 pp. 469-474
ISSN:
0253-6269
Subject:
Castanea crenata; Western blotting; adhesion; chestnut shells; enzyme-linked immunosorbent assay; ethanol; fibroblasts; fibronectins; fluorescent antibody technique; mice; staining; East Asia
Abstract:
... The inner shell of the chestnut (Castanea crenata S. et Z., Fagaceae) has been used as an anti-wrinkle/skin firming agent in East Asia, and preliminary experiments have found that a 70% ethanol extract from this plant material can prevent cell detachment of skin fibroblasts from culture plates. In order to examine the molecular mechanisms underlying this phenomenon, its effects on the expression o ...
DOI:
10.1007/BF02976604

22. Eicosapentaenoic acid promotes apoptosis in Ramos cells via activation of caspase-3 and -9

Author:
Hilde Heimli; Camilla Giske; Soheil Naderi; Christian A. Drevon; Kristin Hollung
Source:
Lipids 2002 v.37 no.8 pp. 797-802
ISSN:
0024-4201
Subject:
DNA; NAD ADP-ribosyltransferase; Western blotting; apoptosis; caspase-3; caspase-8; cell cycle checkpoints; cell proliferation; death; eicosapentaenoic acid; flow cytometry; leukemia; neoplasm cells; protein synthesis; thymidine; tritium; valine
Abstract:
... Eicosapentaenoic acid (EPA; 20∶5n−3) may reduce the cell number in cultured leukemia/lymphoma cells owing to reduced cell proliferation, induction of cell death, or a combination of these processes. EPA has been shown to promote apoptosis in Ramos cells, and our present study was focused on a possible cell cycle arrest and the pathways by which the apoptotic process is induced. Apoptosis may proce ...
DOI:
10.1007/s11745-002-0963-6

23. Enzymes of dimethylsulfone metabolism and the phylogenetic characterization of the facultative methylotrophs Arthrobacter sulfonivorans sp. nov., Arthrobacter methylotrophus sp. nov., and Hyphomicrobium sulfonivorans sp. nov

Author:
Elena Borodina; Donovan P. Kelly; Peter Schumann; Frederick A. Rainey; Naomi L. Ward-Rainey; Ann P. Wood
Source:
Archives of microbiology 2002 v.177 no.2 pp. 173-183
ISSN:
0302-8933
Subject:
Arthrobacter; Filomicrobium; Hyphomicrobium; Micrococcus; Pedomicrobium; Western blotting; antibodies; cross reaction; dimethyl sulfoxide; enzyme activity; fatty acid composition; menaquinones; metabolism; new species; nucleotide sequences; peptidoglycans; phylogeny; polyacrylamide gel electrophoresis; polypeptides; ribosomal RNA; sequence analysis; sulfite dehydrogenase; sulfur; thiosulfate sulfurtransferase
Abstract:
... Novel methylotrophic Arthrobacter and Hyphomicrobium species are described. Constitutive membrane-associated dimethylsulfone- and dimethylsulfoxide-reductases were found in Arthrobacter methylotrophus strain TGA and Hyphomicrobium sulfonivorans strain S1. Enzyme activities increased during growth with dimethylsulfone or dimethylsulfoxide, respectively, and different ratios of activity with differe ...
DOI:
10.1007/s00203-001-0373-3

24. Expression and processing of megasome cysteine proteinases during Leishmania amazonensis differentiation

Author:
Tânia Ueda-Nakamura; Maria da Conceição Rocha Sampaio; Narcisa L. Cunha-e-Silva; Yara M. Traub-Cseko; Wanderley de Souza
Source:
Parasitology research 2002 v.88 no.4 pp. 332-337
ISSN:
0932-0113
Subject:
Leishmania amazonensis; Leishmania mexicana; Northern blotting; RNA; Western blotting; amastigotes; cysteine proteinases; enzyme activity; host-parasite relationships; lysosomes; morphometry; promastigotes
Abstract:
... Amastigotes of Leishmania species belonging to the Leishmania mexicana complex exhibit large lysosomes, called megasomes, which are rich in cysteine proteinases. Various aspects of the host-parasite interaction, the differentiation process as well as intracellular survival, have been attributed to these proteinases. The in vitro differentiation from promastigote to amastigote forms of Leishmania a ...
DOI:
10.1007/s00436-001-0516-0
PubMed:
11999020

25. Expression of Infectious Laryngotracheitis Virus Glycoproteins in Escherichia coli and Their Application in Enzyme-Linked Immunosorbent Assay

Author:
Chang, Poa-Chun; Chen, Kwan-Ting; Shien, Jui-Hung; Shieh, Happy K.
Source:
Avian diseases 2002 v.46 no.3 pp. 570-580
ISSN:
0005-2086
Subject:
chickens; Gallid alphaherpesvirus 1; protein synthesis; specific pathogen-free animals; antigens; antibodies; flocks; vaccines; Western blotting; enzyme-linked immunosorbent assay; nickel; bird diseases; antiserum; glycoproteins; Escherichia coli
Abstract:
... Three glycoproteins of infectious laryngotracheitis virus (ILTV), gC, gE, and gp60, were expressed in Escherichia coli as fusion proteins with a 6-histidine tag at their amino termini. The proteins expressed, designated as r-gC, r-gp60, and r-gE, all retain their antigenicity, as revealed by Western blot with chicken antiserum against ILTV. However, only r-gp60 and r-gE, but not r-gC, were found t ...
DOI:
10.1637/0005-2086(2002)046[0570:EOILVG]2.0.CO;2

26. Expression of a depolymerase gene in transgenic pears increased only slightly their fire blight resistance

Author:
Malnoy, M.; Chevreau, E.; Brisset, M.N.
Source:
Acta horticulturae 2002 v. no.590 pp. 401-405
ISSN:
0567-7572
Subject:
Agrobacterium radiobacter; Erwinia amylovora; Pyrus communis; Western blotting; acclimation; bacteriophages; clones; diploidy; exopolysaccharides; gene expression; genes; genetic engineering; greenhouses; host plants; mutants; pears; proteins; reverse transcriptase polymerase chain reaction; transcription (genetics); virulent strains
Abstract:
... Fire blight, caused by Erwinia amylovora, is a major disease of pear (Pyrus communis L.). Different strategies to enhance fire blight resistance of pear through genetic engineering are currently studied in our group. Among them, transformation of pear with a depolymerase gene from a bacteriophage has been investigated. This gene encodes an enzyme that degrades the capsular exopolysaccharide (EPS) ...
DOI:
10.17660/ActaHortic.2002.590.62

27. Expression of the Pycnoporus cinnabarinus laccase gene in Aspergillus niger and characterization of the recombinant enzyme

Author:
Eric Record; Peter J. Punt; Mohamed Chamkha; Marc Labat; Cees A. M. J. J. van den Hondel; Marcel Asther
Source:
European journal of biochemistry 2002 v.269 no.2 pp. 602-609
ISSN:
0014-2956
Subject:
Aspergillus niger; Pycnoporus; Western blotting; ammonium sulfate; complementary DNA; culture media; fungi; gene overexpression; genes; glucan 1,4-alpha-glucosidase; glyceraldehyde-3-phosphate dehydrogenase; industrial applications; laccase; molecular weight; promoter regions; recombinant proteins; secretion; signal peptide; ultrafiltration
Abstract:
... Pycnoporus cinnabarinus laccase lac1 gene was overexpressed in Aspergillus niger, a well‐known fungal host producing a large amount of homologous or heterologous enzymes for industrial applications. The corresponding cDNA was placed under the control of the glyceraldehyde‐3‐phosphate dehydrogenase promoter as a strong and constitutive promoter. The laccase signal peptide or the glucoamylase ...
DOI:
10.1046/j.0014-2956.2001.02690.x

28. Expression of the mouse metallothionein mutant ββ-cDNA in the lettuces (Lactuca sativa L.)

Author:
Zuo Xiaofeng; Zhang Yukun; Wu Beibei; Chang Xing; Ru Binggen
Source:
Chinese science bulletin 2002 v.47 no.7 pp. 558-562
ISSN:
1001-6538
Subject:
Lactuca sativa; Southern blotting; Western blotting; genes; herbicides; lettuce; metallothionein; mice; mutants; polymerase chain reaction; sexual reproduction; zinc
Abstract:
... Mouse metallothionein (MT) domain mutant ββ-cDNA gene has been inserted into the plant expression vector pGPTVd35S which has herbicide (PPT)resistance gene bar as a selective marker. The chimeric gene was introduced into the lettuce (Lactuca sativa L. cv Salinas 88) by Agrobacterium-mediated transformation. PCR and Southern blot analysis of some putative transformants indicated that the introduced ...
DOI:
10.1360/02tb9128

29. FIV vaccine development and its importance to veterinary and human medicine: a review: FIV vaccine 2002 update and review

Author:
Uhl, E.W.; Heaton-Jones, T.G.; Pu, R.; Yamamoto, J.K.
Source:
Veterinary immunology and immunopathology 2002 v.90 no.3-4 pp. 113-132
ISSN:
0165-2427
Subject:
Feline immunodeficiency virus; Human immunodeficiency virus; USDA; Western blotting; acquired immunodeficiency syndrome; cats; cross reaction; diagnostic techniques; human diseases; humans; industry; medicine; models; vaccine development; vaccines; veterinary medicine
Abstract:
... Feline immunodeficiency virus (FIV) is a natural infection of domestic cats that results in acquired immunodeficiency syndrome resembling human immunodeficiency virus (HIV) infection in humans. The worldwide prevalence of FIV infection in domestic cats has been reported to range from 1 to 28%. Hence, an effective FIV vaccine will have an important impact on veterinary medicine in addition to being ...
DOI:
10.1016/S0165-2427(02)00227-1
PubMed:
12459160
PubMed Central:
PMC7119750

30. Functional Expression of Chitinase and Chitosanase, and Their Effects on Morphologies in the Yeast Schizosaccharomyces pombe

Author:
Kumiko SHIMONO; Hideyuki MATSUDA; Makoto KAWAMUKAI
Source:
Bioscience, biotechnology, and biochemistry 2002 v.66 no.5 pp. 1143-1147
ISSN:
1347-6947
Subject:
Enterobacter; Mitsuaria chitosanitabida; Schizosaccharomyces pombe; Western blotting; adipocytes; animal viruses; chitinase; chitosanase; gene expression; genes; glutathione transferase; molecular cloning; promoter regions; protein synthesis; secretion; transcription (genetics); yeasts
Abstract:
... The chitinase A (chiA) gene from Enterobacter sp. G-1 and the chitosanase A (choA) gene from Matsuebacter chitosanotabidus 3001 were expressed separately and simultaneously in the yeast Schizosaccharomyces pombe. The chiA gene was placed under the transcriptional control of the nmt1 promoter with the glutathione S transferase (GST), and the choA gene was expressed by the human cytomegalovirus (hCM ...
DOI:
10.1271/bbb.66.1143

31. Functional analysis of a small heat shock/α‐crystallin protein from Artemia franciscana: Oligomerization and thermotolerance

Author:
Julie A. Crack; Marc Mansour; Yu Sun; Thomas H. MacRae
Source:
European journal of biochemistry 2002 v.269 no.3 pp. 933-942
ISSN:
0014-2956
Subject:
Artemia franciscana; Escherichia coli; Western blotting; antibodies; bacteria; complementary DNA; diapause; epitopes; heat; heat tolerance; hypoxia; molecular chaperones; polymerase chain reaction; polypeptides
Abstract:
... Oviparously developing embryos of the brine shrimp, Artemia franciscana, synthesize abundant quantities of a small heat shock/α‐crystallin protein, termed p26. Wild‐type p26 functions as a molecular chaperone in vitro and is thought to help encysted Artemia embryos survive severe physiological stress encountered during diapause and anoxia. Full‐length and truncated p26 cDNA derivatives were ...
DOI:
10.1046/j.0014-2956.2001.02726.x

32. Gene Knock-down in Rainbow Trout Embryos Using Antisense Morpholino Phosphorodiamidate Oligonucleotides

Author:
Surintorn Boonanuntanasarn; Goro Yoshizaki; Yutaka Takeuchi; Tetsuro Morita; Toshio Takeuchi
Source:
Marine biotechnology 2002 v.4 no.3 pp. 256-266
ISSN:
1436-2228
Subject:
genetic traits; Western blotting; genetically modified organisms; dose response; skeletal muscle; gene expression; hatching; actin; embryogenesis; trout; oligonucleotides; messenger RNA; models; toxicity; eggs; green fluorescent protein; translation (genetics); Oncorhynchus mykiss; reverse transcriptase polymerase chain reaction; therapeutics; codons
Abstract:
... : Gene knock-down technology using antisense molecules has many applications for studying gene function, disrupting undesirable genetic traits, as well as providing effective therapy for a number of viral diseases. Encouraged by these applications, we developed a gene knock-down technique to interfere with gene expression using transgenic rainbow trout expressing the green fluorescent protein (GFP ...
DOI:
10.1007/s10126-002-0017-x

33. High Level of Field Resistance of Transgenic Tobaccos Induced by Integrated Potato Virus Y Coat Protein Gene

Author:
R. Józsa; Z. Stasevski; E. Balázs
Source:
Acta phytopathologica et entomologica Hungarica 2002 v.37 no.4 pp. 311-316
ISSN:
0238-1249
Subject:
Agrobacterium; Potato virus Y; Western blotting; breeding lines; coat proteins; cultivars; flora; genes; greenhouses; nucleic acid hybridization; planting; polymerase chain reaction; progeny; tobacco; transgenic plants; virulence
Abstract:
... The coat protein gene (CP) from a highly virulent, necrotic and dominant strain of potato virus Y (PVY) originated from the Hungarian flora has been engineered via Agrobacterium infection into different Hungarian tobacco breeding lines and cultivars. The integration of the CP was confirmed by polymerase chain reaction (PCR) using genomic prepa­ra­tions. The transcription and the expression of the ...
DOI:
10.1556/APhyt.37.2002.4.1

34. High-level production of the non-cariogenic sucrose isomer palatinose in transgenic tobacco plants strongly impairs development

Author:
Frederik Börnke; Mohammad Hajirezaei; Dieter Heineke; Michael Melzer; Karin Herbers; Uwe Sonnewald
Source:
Planta 2002 v.214 no.3 pp. 356-364
ISSN:
0032-0935
Subject:
Erwinia rhapontici; Western blotting; bacteria; carbon; extracellular fluids; flowers; food production; fractionation; genes; isomaltulose; isomers; leaves; octopine; potatoes; protein secretion; protein synthesis; signal peptide; sucrose; tobacco; transgenic plants
Abstract:
... Palatinose (isomaltulose, 6-O-α-D-glucopyranosyl-D-fructose) is a structural isomer of sucrose which is produced from sucrose by some bacterial strains as a reserve material during periods of low carbon availability. The ability to synthesise palatinose is not only advantageous for the bacteria but is also of industrial interest since palatinose is used as a sucrose substitute in food production. ...
DOI:
10.1007/s004250100629
PubMed:
11855640

35. Identification of a receptor-binding domain of Bordetella dermonecrotic toxin

Author:
Matsuzawa, T.; Kashimoto, T.; Katahira, J.; Horiguchi, Y.
Source:
Infection and immunity 2002 v.70 no.7 pp. 3427-3432
ISSN:
0019-9567
Subject:
Bordetella; Western blotting; actin; amino acids; clones; focal adhesions; guanosinetriphosphatase; hybridomas; monoclonal antibodies; mutants; poisoning
Abstract:
... Bordetella dermonecrotic toxin (DNT) stimulates the assembly of actin stress fibers and focal adhesions by deamidating or polyaminating Gln63 of the small GTPase Rho. DNT is an A-B toxin which is composed of an N-terminal receptor-binding (B) domain and a C-terminal enzymatically active (A) domain. In this study, to analyze the functional and structural organization of DNT, we prepared 10 clones o ...
DOI:
10.1128/IAI.70.7.3427-3432.2002
PubMed:
12065482
PubMed Central:
PMC128056

36. Identification of hepatopoietin dimerization, its interacting regions and alternative splicing of its transcription

Author:
Yong Li; Kaihua Wei; Chengrong Lu; Yingxian Li; Ming Li; Guichun Xing; Handong Wei; Qingming Wang; Jizhong Chen; Chutse Wu; Huipeng Chen; Songcheng Yang; Fuchu He
Source:
European journal of biochemistry 2002 v.269 no.16 pp. 3888-3893
ISSN:
0014-2956
Subject:
Western blotting; alternative splicing; amino acids; cDNA libraries; complementary DNA; cytoplasm; dimerization; disulfide bonds; hepatocytes; humans; liver; mass spectrometry; molecular weight; mutants; transcription factors; two hybrid system techniques
Abstract:
... Hepatopoietin (HPO) is a novel human hepatotrophic growth factor. Recently, we demonstrated that the extracellular ligand form of HPO can stimulate proliferation of hepatic cells via its specific receptor, which is on the surface of these cells. Also, the intracellular form of HPO potentiates the transcriptional factor AP‐1. Intriguingly, a variety of HPO complexes with different molecular masse ...
DOI:
10.1046/j.1432-1033.2002.03054.x

37. Identification of monoclonal antibodies that cross-react with cytokines from different animal species

Author:
Pedersen, L.G.; Castelruiz, Y.; Jacobsen, S.; Aasted, B.
Source:
Veterinary immunology and immunopathology 2002 v.88 no.3-4 pp. 111-122
ISSN:
0165-2427
Subject:
Western blotting; cattle; dogs; flow cytometry; goats; granulocyte-macrophage colony-stimulating factor; horses; humans; interferon-gamma; interleukin-2; interleukin-4; interleukin-6; interleukin-8; mink; monoclonal antibodies; mononuclear leukocytes; rabbits; sheep; swine; tumor necrosis factor-alpha
Abstract:
... Eleven monoclonal antibodies specific for ovine, bovine and human cytokines were investigated by flow cytometry for cross-reactivities with cytokines produced by peripheral blood mononuclear cells (PBMCs) from sheep, cattle, goat, swine, horse, dog, mink, rabbit and human. Four antibodies specific for IL-4, IL-8, IFN-γ and TNF-α cross-reacted with cytokines from a majority of the species investiga ...
DOI:
10.1016/S0165-2427(02)00139-3

38. Immunization of cattle with synthetic peptides derived from the Boophilus microplus gut protein (Bm86)

Author:
Patarroyo, J.H.; Portela, R.W.; Castro, R.O. De; Pimentel, J. Couto; Guzman, F.; Patarroyo, M.E.; Vargas, M.I.; Prates, A.A.; Mendes, M.A. Dias
Source:
Veterinary immunology and immunopathology 2002 v.88 no.3-4 pp. 163-172
ISSN:
0165-2427
Subject:
Rhipicephalus microplus; Western blotting; antibodies; cattle; digestive system; females; glycoproteins; immune response; immunization; immunoglobulin G; oviposition; saponins; synthetic peptides; ticks
Abstract:
... Three synthetic peptides (SBm4912, SBm7462 and SBm19733), derived from the Bm86 glycoprotein from Boophilus microplus gut, were constructed and used to immunize cattle from a tick-free area. The immunized animals received three subcutaneous doses of the peptides, with saponin as adjuvant, at 30-day intervals. The immune response was evaluated by IgG elicited against the peptides by the detection o ...
DOI:
10.1016/S0165-2427(02)00154-X

39. Impaired glucose metabolism in the heart of obese Zucker rats after treatment with phorbol ester

Author:
Morabito, D.; Montessuit, C.; Rosenblatt-Velin, N.; Lerch, R.; Vallotton, M.B.; Lang, U.
Source:
International journal of obesity 2002 v.26 no.3 pp. 327-334
ISSN:
0307-0565
Subject:
Western blotting; cytosol; glucose; glycolysis; heart; isozymes; metabolic studies; obesity; oxidation; protein kinase C; rats
Abstract:
... OBJECTIVE: To investigate the influence of obesity on the regulation of myocardial glucose metabolism following protein kinase C (PKC) activation in obese (fa/fa) and lean (Fa/?) Zucker rats. DESIGN: Isolated hearts obtained from 17-week-old lean and obese Zucker rats were perfused with 200 nM phorbol 12-myristate 13-acetate (PMA) for different time periods prior to the evaluation of PKC and GLUT- ...
DOI:
10.1038/sj/ijo/0801881
PubMed:
11896487

40. In vitro proteolysis of myofibrillar and sarcoplasmic proteins of European sea bass (Dicentrarchus Labrax L) by an endogenous m-calpain

Author:
Verrez-Bagnis, Veronique; Ladrat, Christine; Noelle, Joelle; Fleurence, Joel
Source:
Journal of the science of food and agriculture 2002 v.82 no.11 pp. 1256-1262
ISSN:
0022-5142
Subject:
Dicentrarchus labrax; Western blotting; bass; denaturation; desmin; digestion; molecular weight; myofibrillar proteins; myosin heavy chains; polyacrylamide gel electrophoresis; polypeptides; proteolysis; skeletal muscle
Abstract:
... The effects of m-calpain isolated from the skeletal muscle of sea bass on sarcoplasmic and myofibrillar proteins isolated from the same tissue were examined in vitro. Incubation of sarcoplasmic proteins with m-calpain resulted in only a slight decrease (0.7 kDa) in the molecular weight (MW) of a 26.5 kDa protein. Degradation of myofibrils, monitored by quantification of TCA-soluble peptides genera ...
DOI:
10.1002/jsfa.1172

41. In vivo accumulation of Broad bean wilt virus 2 VP37 protein and its ability to bind single-stranded nucleic acid

Author:
Y. J. Qi; X. P. Zhou; X. Z. Huang; G. X. Li
Source:
Archives of virology 2002 v.147 no.5 pp. 917-928
ISSN:
0304-8608
Subject:
Broad bean wilt virus 2; Chenopodium quinoa; Escherichia coli; RNA; Western blotting; crosslinking; gels; leaves; plant viruses; proteins; sequence analysis; single-stranded DNA; sodium chloride; wilting
Abstract:
... The VP37 protein encoded by the RNA2 of Broad bean wilt virus 2 (BBWV2) was overexpressed in Escherichia coli. The protein was purified and a polyclonal antibody specific for the protein was produced. Time course studies by Western blot assays in BBWV2-infected Chenopodium quinoa leaves showed that the VP37 protein was present in cells of the inoculated leaves by 12 h post inoculation and in cells ...
DOI:
10.1007/s00705-001-0782-2
PubMed:
12021864

42. Incorporation of N-acetylneuraminic acid into Haemophilus somnus lipooligosaccharide (LOS): enhancement of resistance to serum and reduction of LOS antibody binding

Author:
Inzana, T.J.; Glindemann, G.; Cox, A.D.; Wakarchuk, W.; Howard, M.D.
Source:
Infection and immunity 2002 v.70 no.9 pp. 4870-4879
ISSN:
0019-9567
Subject:
Haemophilus influenzae; Haemophilus somni; Limax; Neisseria gonorrhoeae; Western blotting; agglutinins; antiserum; blood serum; capillary electrophoresis; culture media; enzyme immunoassays; epitopes; glycosyltransferases; immune system; lectins; lipooligosaccharides; mass spectrometry; meningoencephalitis; molecular weight; monoclonal antibodies; mutants; pneumonia; sialic acid; sialidase; virulence
Abstract:
... Haemophilus somnus isolates from cases of thrombotic meningoencephalitis, pneumonia, and other disease sites are capable of undergoing a high rate of phase variation in the oligosaccharide component of their lipooligosaccharides (LOS). In contrast, the LOS of commensal strains isolated from the normal reproductive tract phase vary little or not at all. In addition, the LOS of H. somnus shares cons ...
DOI:
10.1128/IAI.70.9.4870-4879.2002
PubMed:
12183531
PubMed Central:
PMC128230

43. Inflamatory cytokines enhance the interaction of Mannheimia haemolytica leukotoxin with bovine peripheral blood neutrophils in vitro

Author:
Leite, F.; O'Brien, S.; Sylte, M.J.; Page, T.; Atapattu, D.; Czuprynski, C.J.
Source:
Infection and immunity 2002 v.70 no.8 pp. 4336-4343
ISSN:
0019-9567
Subject:
CD18 antigen; Mannheimia haemolytica; Pasteurella; Western blotting; apoptosis; caspase-3; cattle; cytolysis; cytotoxicity; flow cytometry; interferon-gamma; interleukin-1beta; iodine; leukotoxins; monoclonal antibodies; neutrophils; pathogenesis; pneumonic pasteurellosis; propidium; quantitative polymerase chain reaction; staining; tumor necrosis factor-alpha; virulence
Abstract:
... Mannheimia (Pasteurella) haemolytica A1 produces several virulence factors that play an important role in the pathogenesis of bovine pneumonic pasteurellosis. Foremost among these is a leukotoxin (LKT) that specifically kills ruminant leukocytes. Recent evidence suggests that M. haemolytica LKT binding to bovine leukocytes is mediated by the beta2-integrin CD11a/CD18 (lymphocyte function-associate ...
DOI:
10.1128/IAI.70.8.4336-4343.2002
PubMed:
12117943
PubMed Central:
PMC128205

44. Involvement of 3-hydroxy-3-methylglutaryl coenzyme a reductase in cell division in the pericarp, which determines melon fruit size

Author:
Kobayashi, T.; Kato-Emori, S.; Tomita, K.; Ezura, H.
Source:
Acta horticulturae 2002 v. no.588 pp. 35-38
ISSN:
0567-7572
Subject:
tomatoes; pericarp; yeasts; flow cytometry; cell division; Western blotting; Cucumis melo subsp. melo var. cantalupo; avocados; genes; mutants; fruiting
Abstract:
... The fruit size of melon (Cucumis melo var. reticulatus) is determined by the number of pericarp cells that are defined during early fruit development. It has been reported that the expression and activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) is required for early fruit growth in tomato and avocado. In this study, we cloned a melon HMGR gene and investigated its involvement in ...
DOI:
10.17660/ActaHortic.2002.588.2

45. Kinetic properties of bifunctional 6‐phosphofructo‐2‐kinase/ fructose‐2,6‐bisphosphatase from spinach leaves

Author:
Jonathan E. Markham; Nicholas J. Kruger
Source:
European journal of biochemistry 2002 v.269 no.4 pp. 1267-1277
ISSN:
0014-2956
Subject:
6-phosphofructo-2-kinase; Baculoviridae; Escherichia coli; Spinacia oleracea; Spodoptera frugiperda; Western blotting; adenosine triphosphate; antibodies; complementary DNA; cytosol; esters; fructose; fructose 6-phosphate; fructose-2,6-bisphosphate 2-phosphatase; gel chromatography; leaves; metabolites; molecular weight; phosphates; spinach
Abstract:
... A cDNA encoding 6‐phosphofructo‐2‐kinase/fructose‐2,6‐bisphosphatase was isolated from a Spinacia oleracea leaf library and used to express a recombinant enzyme in Escherichia coli and Spodoptera frugiperda cells. The insoluble protein expressed in E. coli was purified and used to raise antibodies. Western blot analysis of a protein extract from spinach leaf showed a single band of 90. ...
DOI:
10.1046/j.1432-1033.2002.02771.x

46. Legume proteins of the vicilin family are more immunogenic than those of the legumin family in weaned piglets

Author:
Salgado, P.; Freire, J.B.; Ferreira, R.B.; Seabra, M.; Teixeira, A.R.; Toullec, R.; Lalles, J.P.
Source:
Food and agricultural immunology 2002 v.14 no.1 pp. 51-63
ISSN:
0954-0105
Subject:
piglets; soybean meal; peas; faba beans; chickpeas; vicilin; legumin; antigens; piglet feeding; immunoglobulin G; antibody formation; blood plasma; glycinin; beta-conglycinin; enzyme-linked immunosorbent assay; Western blotting
DOI:
10.1080/09540100220137664

47. Mannheimia haemolytica serotype 1 and Pasteurella trehalosi serotype 10 culture supernatants contain fibrinogen-binding proteins

Author:
McNeil, Heather J.; Shewen, Patricia E.; Lo, Reggie Y.C.; Conlon, Jennifer A.; Miller, Michael W.
Source:
Veterinary immunology and immunopathology 2002 v.90 no.1-2 pp. 107-110
ISSN:
0165-2427
Subject:
Mannheimia haemolytica serotype 1; Pasteurella trehalosi; Western blotting; disease control; fibrinogen; pneumonia; serotypes; virulence
Abstract:
... Fibrinogen-binding proteins were found in the culture supernatants of Mannheimia haemolytica serotype 1 (ATCC 43270) and Pasteurella trehalosi serotype 10 (ECO-100). Sheep fibrinogen was biotinylated and shown to bind to proteins in the culture supernatants by modified western blot. Fibrinogen-binding proteins in the culture supernatant may be important virulence factors leading to the characteris ...
DOI:
10.1016/S0165-2427(02)00222-2

48. Molecular characterization of MRG19 of Saccharomyces cerevisiae: Implication in the regulation of galactose and nonfermentable carbon source utilization

Author:
Firdous A. Khanday; Maitreyi Saha; Paike Jayadeva Bhat
Source:
European journal of biochemistry 2002 v.269 no.23 pp. 5840-5850
ISSN:
0014-2956
Subject:
Saccharomyces cerevisiae; Western blotting; beta-galactosidase; carbon; galactose; gene expression; genes; glucose; glycerol; green fluorescent protein; nuclear localization signals; oxygen; phenotype
Abstract:
... We have reported previously that multiple copies of MRG19 suppress GAL genes in a wild‐type but not in a gal80 strain of Saccharomyces cerevisiae. In this report we show that disruption of MRG19 leads to a decrease in GAL induction when S. cerevisiae is induced with 0.02% but not with 2.0% galactose. Disruption of MRG19 in a gal3 background (this strain shows long‐term adaptation phenotype) ...
DOI:
10.1046/j.1432-1033.2002.03303.x

49. Mutational analysis of the ethylene receptor ETR1. Role of the histidine kinase domain in dominant ethylene insensitivity

Author:
Gamble, R.L.; Qu, X.; Schaller, G.E.
Source:
Plant physiology 2002 v.128 no.4 pp. 1428-1438
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; transgenic plants; seedlings; seedling growth; ethylene; hormone receptors; signal peptide; enzyme activity; gene expression; biochemical pathways; Saccharomyces cerevisiae; plant morphology; nucleotide sequences; signal transduction; binding sites; Western blotting
Abstract:
... The ethylene receptor family of Arabidopsis consists of five members, one of these being ETR1. The N-terminal half of ETR1 contains a hydrophobic domain responsible for ethylene binding and membrane localization. The C-terminal half of the polypeptide contains domains with homology to histidine (His) kinases and response regulators, signaling motifs originally identified in bacteria. The role of t ...
DOI:
10.1104/pp.010777
PubMed:
11950991
PubMed Central:
PMC154270

50. Optimisation of cut-off titres in Toxoplasma gondii specific ELISA and IFAT in dog sera using immunoreactivity to SAG-1 antigen as a molecular marker of infection

Author:
Silva, N.M.; Lourenco, E.V.; Silva, D.A.O.; Mineo, J.R.
Source:
Veterinary journal 2002 v.163 no.1 pp. 94-98
ISSN:
1090-0233
Subject:
dogs; Toxoplasma gondii; fluorescent antibody technique; antigens; enzyme-linked immunosorbent assay; Western blotting
PubMed:
11749142