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You searched for: Publication Year 1997 Remove constraint Publication Year: 1997 Subject Brassica napus Remove constraint Subject: Brassica napus Subject term beta-glucuronidase Remove constraint Subject term: beta-glucuronidase
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1. Importance of the chiral centers of jasmonic acid in the responses of plants. Activities and antagonism between natural and synthetic analogs

Author:
Holbrook, L.; Tung, P.; Ward, K.; Reid, D.M.; Abrams, S.; Lamb, N.; Quail, J.W.; Moloney, M.M.
Source:
Plant physiology 1997 v.114 no.2 pp. 419-428
ISSN:
0032-0889
Subject:
Helianthus annuus; Brassica napus; measurement; chemical degradation; dose response; jasmonic acid; gene expression; Triticum aestivum; Nicotiana tabacum; esters; stereochemistry; enzyme activity; chemical structure; seed germination; transgenic plants; cotyledons; proteinase inhibitors; reporter genes; chemical constituents of plants; chlorophyll; beta-glucuronidase
Abstract:
... The importance of the two chiral centers at C-3 and C-7 in the molecular structure of jasmonic acid in plant responses was investigated. We separated methyl jasmonate (MeJA) into (3R)- and (3S)-isomers with a fixed stereochemistry at C-3, but epimerization at C-7 is possible. The four isomers of the nonepimerizable analog 7-methyl MeJA were synthesized. These six esters and their corresponding aci ...

2. Transient expression and oil body targeting of an Arabidopsis oleosin-GUS reporter fusion protein in a range of oilseed embryos

Author:
Abenes, M.; Holbrook, L.; Moloney, M.
Source:
Plant cell reports 1997 v.17 no.1 pp. 1-7
ISSN:
0721-7714
Subject:
Brassica napus; gene transfer; Arabidopsis thaliana; embryo (plant); binding proteins; beta-glucuronidase; enzyme activity; reporter genes; gene expression; developmental stages; histochemistry; transgenic plants
Abstract:
... An Arabidopsis oleosin gene including 860 bp of promoter as well as coding sequence was fused to the reporter, beta-glucuronidase (GUS). In transient expression assays following particle bombardment, the Arabidopsis gene in both transcriptional and translational fusions with GUS was functional in a range of oilseed embryos as determined histochemically and fluorimetrically. Significantly, upon hom ...
DOI:
10.1007/s002990050341