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... A cDNA encoding the oleate 12-hydroxylase from castor bean (Ricinus communis L.) has previously been shown to direct the synthesis of small amounts of ricinoleic acid (12-hydroxyoctadec-cis-9-enoic acid) in seeds of transgenic tobacco plants. Expression of the cDNA under control of the Brassica napus napin promoter in transgenic Arabidopsis thaliana plants resulted in the accumulation of up to 17% ...
Carthamus tinctorius; seeds; chemical reactions; chemical constituents of plants; unsaturated fatty acids; plant fats and oils; biochemical pathways; structure-activity relationships; Brassica napus; acetate-CoA ligase; saturated fatty acids; enzyme activity
Abstract:
... The substrate specificities and selectivities of acyl-CoA synthetases from maturing oilseeds were investigated to reveal fatty acid structures that the enzymes recognize. The synthetases from rapeseed (Brassica napus) and castor bean (Ricinus communis) activated palmitic acid 16:0 most rapidly among the saturated fatty acids tested. Native unsaturated fatty acids, oleic 18:1 cis-9, linoleic 18:2 c ...
... In northern Idaho, winter rapeseed (Brassica napus L.) usually is seeded on summer fallow in early to mid-August at 4.5 to 13.5 kg seed ha⁻¹. A 2-yr study was conducted at Moscow, ID, to quantify crop performance as influenced by date of seeding and seeding rate. In the 1991–1992 and 1992–1993 growing seasons, high-erucic-acid winter rapeseed cultivars, ‘Dwarf Essex’ and ‘Bridger’, were seeded on ...
ribulose-bisphosphate carboxylase; cultivars; carbon dioxide; chlorophyll; carboxylation; fluorescence; enzyme activity; electron transfer; photosystem II; Brassica napus; irradiation; chemical constituents of plants; ultraviolet radiation; stomata
Abstract:
... Apex and Bristol cultivars of oilseed rape (Brassica napus) were irradiated with 0.63 W m-2 of UV-B over 5 d. Analyses of the response of net leaf carbon assimilation to intercellular CO2 concentration were used to examine the potential limitations imposed by stomata, carboxylation velocity and capacity for regeneration of ribulose 1,5-bisphosphate on leaf photosynthesis. Simultaneous measurements ...
... Improved microscale methods for analyzing individual carbon atoms of long- and very-long-chain fatty acids are described. These methods allow quantitation of radiolabel in at least 50% of individual carbon atoms from the carboxyl terminal of such fatty acids. These methods have been used to study metabolic pathways leading to long- and very-long-chain fatty acids of seeds. The approach begins with ...
... Spring canola (Brassica napus L. and B. rapa L.) is sometimes planted when soil temperatures are below the optimum, causing farmers to have stand losses because of seed rotting in cold soil. Knowledge of the growing-degree hours (GDH) required for emergence of canola from different planting depths could help producers decide when and how deep to plant this crop. Our objectives were to quantify the ...
... Two different cytoplasmic male-sterility (CMS) systems, nap and pol, are found in the oilseed rape (canola) species Brassica napus. Physical mapping studies have previously shown that organizational differences between the sterile pol and fertile cam mitochondrial genomes are restricted to a relatively small region immediately upstream of the atp6 gene. An approximately 4.5-kb pol mtDNA segment co ...
... Little biochemical information is available on carbohydrate metabolism in developing canola (Brassica napus L.) silique (pod) wall and seed tissues. This research examines the carbohydrate contents and sucrose (Suc) metabolic enzyme activities in different aged silique wall and seed tissues during oil filling. The silique wall partitioned photosynthate into Suc over starch and predominantly accumu ...
... The cleavage of pheophorbide (Pheide) a into primary fluorescent chlorophyll (Chl) catabolites (pFCCs) in senescent chloroplasts was investigated. Chloroplast preparations isolated fromsenescent canola (Brassica napus) cotyledons exhibited light-dependent production of pFCC when assay mixtures were supplemented with ferredoxin (Fd). pFCC production in detergent-solubilized membranes was dependent ...
... Using fluorescence in situ hybridization, we located ribosomal DNA loci on prometaphase chromosomes of the diploid species Brassica rapa and Brassica oleracea and their amphidiploid Brassica napus. Based on comparisons of chromosome morphology and hybridization patterns, we characterized the individual B. napus rDNA loci according to their presumed origins in the Brassica A and C genomes. As repor ...
... The patterns of chromosome pairing and recombination in two contrasting Brassica napus F1 hybrids were deduced. One hybrid was from a winter oilseed rape (WOSR) X spring oilseed rape cross, the other from a resynthesized B. napus X WOSR cross. Segregation at 211 equivalent loci assayed in the population derived from each hybrid produced two collinear genetic maps. Alignment of the maps indicated t ...
... The temporal distribution of ATP/citrate lyase (ACL) activity in developing seeds of Brassica napus L. closely paralleled both that of acetyl-CoA carboxylase and the overall rate of lipid biosynthesis. Maximum ACL activities (250 nmol acetyl-CoA formed min⁻¹·g fresh seed) were recorded between 35 to 42 d after pollination and, if the in vitro data could be extrapolated to the situation in vivo, co ...
... The RbpA3 protein of Anabaena variabilis contains one RNA-binding domain with a carboxy-terminal glycine-rich domain. Levels of two transcripts of the rbpA3 gene were differentially regulated by growth temperature. The RbpA3 protein is related to the RbpC protein within the group of cyanobacterial Rbps. ...
... The influence of the anti-fungal agent phosphonate (Phi) on the response of oilseed rape (Brassica napus L. cv. Jet Neuf) cell suspensions to inorganic phosphate (Pi) starvation was examined. Subculture of the cells for 7 d in the absence of Pi increased acid phosphatase (APase; EC 3.1.3.2) and pyrophosphate (PPi)-dependent phosphofructokinase (PFP; EC 2.7.1.90) activities by 4.5- and 2.8-fold, re ...