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You searched for: Publication Year 1997 Remove constraint Publication Year: 1997 Subject Nicotiana tabacum Remove constraint Subject: Nicotiana tabacum
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1. A 146 bp fragment of the tobacco Lhcb1*2 promoter confers very-low-fluence, low-fluence and high-irradiance responses of phytochrome to a minimal CaMV 35S promoter

Author:
Pablo D. Cerdán; Roberto J. Staneloni; Jorge J. Casal; Rodolfo A. Sánchez
Source:
Plant molecular biology 1997 v.33 no.2 pp. 245-255
ISSN:
0167-4412
Subject:
Cauliflower mosaic virus; Nicotiana tabacum; beta-glucuronidase; far-red light; gene expression; genes; light intensity; phytochrome; promoter regions; red light; seedlings; tobacco; transgenic plants
Abstract:
... The occurrence of very-low-fluence responses (VLFR), low-fluence responses (LFR) and high-irradiance responses (HIR) of phytochrome was investigated for the expression of the gene of β-glucuronidase (gusA) under the control of the tobacco Lhcb1*2 promoter, in etiolated transgenic tobacco seedlings. The activity of β-glucuronidase (GUS) showed biphasic responses to the calculated proportion of Pf ...
DOI:
10.1023/A:1005784407282

2. AC Cheng flue-cured tobacco

Author:
Brandle, J.E.; Ankersmit, J.C.D.; Rogers, W.D.
Source:
Canadian journal of plant science 1997 v.77 no.1 pp. 155-156
ISSN:
0008-4220
Subject:
cultivars; hybrids; somatic hybridization; Nicotiana rustica; Nicotiana tabacum; Canada
DOI:
10.4141/P96-066

3. AC Gayed flue-cured tobacco

Author:
Brandle, J.E.; Rogers, W.D.; Ankersmit, J.C.D.
Source:
Canadian journal of plant science 1997 v.77 no.1 pp. 157-158
ISSN:
0008-4220
Subject:
Thielaviopsis basicola; cultivars; hybrids; Nicotiana debneyi; Nicotiana tabacum; disease resistance; plant pathogenic fungi; genes; Ontario
DOI:
10.4141/P96-067

4. AC Maridel flue-cured tobacco

Author:
Brandle, J.E.; Arsenault, W.; Rogers, W.D.; Ankersmit, J.C.D.
Source:
Canadian journal of plant science 1997 v.77 no.1 pp. 159-160
ISSN:
0008-4220
Subject:
Thielaviopsis basicola; Nicotiana tabacum; plant pathogenic fungi; disease resistance; high-yielding varieties; Prince Edward Island
DOI:
10.4141/P96-077

5. Accumulation of nitrate in the shoot acts as a signal to regulate shoot-root allocation in tobacco

Author:
Scheible, W.R.; Lauerer, M.; Schulze, E.D.; Caboche, M.; Stitt, M.
Source:
plant journal 1997 v.11 no.4 pp. 671-691
ISSN:
0960-7412
Subject:
nitrate reductase; nutrition-genotype interaction; roots; glutamate decarboxylase; shoots; carbohydrate metabolism; nitrogen content; sugars; starch; Nicotiana tabacum; genotype; phosphates; root shoot ratio; ammonium nitrate; leaves; glutamine; nutrient deficiencies; dry matter partitioning; genetic transformation; nutrient availability; mutants; chemical constituents of plants; protein content
Abstract:
... Mutants and transformants of tobacco (Nicotiana tabacum L. cv Gatersleben 1) with decreased expression of nitrate reductase have been used to investigate whether nitrate accumulation in the shoot acts as a signal to alter allocation between shoot and root growth. (a) Transformants with very low (1-3% of wild-type levels) nitrate reductase activity had growth rates, and protein, amino acid and glut ...
DOI:
10.1046/j.1365-313X.1997.11040671.x

6. Actin microfilaments are required for the expression of nonhost resistance in higher plants

Author:
Kobayashi, Y.; Yamada, M.; Kobayashi, I.; Kunoh, H.
Source:
Plant & cell physiology 1997 v.38 no.6 pp. 725-733
ISSN:
0032-0781
Subject:
Podosphaera xanthii; Blumeria graminis f. sp. hordei; Glomerella graminicola; developmental stages; Blumeria graminis f. sp. tritici; plant pathogenic fungi; Pisum sativum; cytochalasin B; Hordeum vulgare; host-parasite relationships; Mycosphaerella pinodes; Nicotiana tabacum; Triticum aestivum; disease resistance; cell wall components; actin; Colletotrichum orbiculare; Cucumis sativus; polymerization; pathogenicity; Corynespora; Erysiphe pisi; plant anatomy
Abstract:
... We investigated the role of actin microfilaments in nonhost resistance of higher plants. Here we present several lines of evidence to indicate that microfilaments are indeed involved in blocking fungal penetration of nonhost plants. Erysiphe pisi, a pathogen of pea, normally fails to penetrate into nonhost plants such as barley, wheat, cucumber and tobacco. When tissues of these nonhost plants wer ...
DOI:
10.1093/oxfordjournals.pcp.a029226

7. Active oxygen species production in tobacco cells elicited by cryptogein

Author:
Simon-Plas, F.; Rusterucci, C.; Milat, M.L.; Humbert, C.; Montillet, J.L.; Blein, J.P.
Source:
Plant, cell and environment 1997 v.20 no.12 pp. 1573-1579
ISSN:
0140-7791
Subject:
proteins; extracellular fluids; free radicals; hydrogen peroxide; oxidoreductases; enzyme inhibitors; fusicoccin; pH; Nicotiana tabacum; alkalinization; plasma membrane; enzyme activity; oxygen; cell suspension culture; adenosinetriphosphatase; defense mechanisms
Abstract:
... We analyse the relationship between active oxygen species (AOS) production and pH changes induced in tobacco cells by cryptogein, a fungal proteinaceous elicitor of defence mechanisms in plants. When tobacco cells were treated with cryptogein, an intracellular acidification, an alkalinization of the extracellular medium and a transient burst of AOS (H2O2) were observed. Treatment of elicited cells ...
DOI:
10.1046/j.1365-3040.1997.d01-45.x

8. Aetiology of tobacco leaf curl in southern Africa

Author:
Paximadis, M.; Rey, M.E.C.
Source:
Annals of applied biology 1997 v.131 no.3 pp. 449-457
ISSN:
0003-4746
Subject:
Tobacco leaf curl Japan virus; etiology; leaves; Nicotiana tabacum; RNA; South Africa; Zimbabwe
DOI:
10.1111/j.1744-7348.1997.tb05172.x

9. Airborne signalling by methyl salicylate in plant pathogen resistance. [Erratum: Apr 17, 1997, v. 386 (6626), p. 738.]

Author:
Shulaev, V.; Silverman, P.; Raskin, I.
Source:
Nature 1997 v.385 no.6618 pp. 718-721
ISSN:
0028-0836
Subject:
salicylates; biological production; Nicotiana tabacum; Tobacco mosaic virus; disease resistance; gene expression; defense mechanisms
Abstract:
... Methyl salicylate, a volatile liquid, also known as oil of wintergreen, is made by a number of plants. Here we show that methyl salicylate is a major volatile compound produced by tobacco plants inoculated with tobacco mosaic virus. Methyl salicylate is synthesized from salicylic acid, a non-volatile chemical signal required for the establishment of acquired resistance and local and systemic induc ...
DOI:
10.1038/385718a0

10. Alfalfa mosaic virus movement protein induces tubules in plant protoplasts

Author:
Zheng, H.; Wang, G.; Zhang, L.
Source:
Molecular plant-microbe interactions 1997 v.10 no.8 pp. 1010-1014
ISSN:
0894-0282
Subject:
protoplasts; animal proteins; recombinant proteins; plasmid vectors; mutation; amino acid sequences; gene expression; Scyphozoa; viral proteins; Nicotiana tabacum; surfaces; transfection; Alfalfa mosaic virus
Abstract:
... The alfalfa mosaic virus (AIMV) movement protein (MP) was fused with the green fluorescence protein (GFP). The MP-GFP fusion was transiently expressed in protoplasts prepared from Nicotiana tabacum, resulting in the production of long, extending, tubular structures protruding from the protoplast surface. Deletions of MP amino acids 1 to 77, 84 to 142, or 226 to 300 all affected tubule formation. H ...
DOI:
10.1094/MPMI.1997.10.8.1010

11. Alternative processing of the maize Ac transcript in Arabidopsis

Author:
Martin, D.J.; Firek, S.; Moreau, E.; Draper, J.
Source:
plant journal 1997 v.11 no.5 pp. 933-945
ISSN:
0960-7412
Subject:
Arabidopsis thaliana; Zea mays; messenger RNA; alternative splicing; chemical reactions; transgenic plants; nucleotidyltransferases; Nicotiana tabacum; Nicotiana plumbaginifolia; introns; transposons
Abstract:
... The successful application of the maize transposable element system Ac/Ds as a genome mutagen in heterologous plant species has recently proved the versatility and power of this technique in plant molecular biology. However, the frequency of Ac/Ds transposition is considerably lower in Arabidopsis thaliana than in most other dicot plant species that have been studied. Since previous research has e ...
DOI:
10.1046/j.1365-313X.1997.11050933.x
PubMed:
9193067

12. Antigens produced in plants by infection with chimeric plant viruses immunize against rabies virus and HIV-1

Author:
Yusibov, V.; Modelska, A.; Steplewski, K.; Agadjanyan, M.; Weiner, D.; Hooper, D.C.; Koprowski, H.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1997 v.94 no.11 pp. 5784-5788
ISSN:
0027-8424
Subject:
coat proteins; recombinant antigens; Tobacco mosaic virus; Rabies lyssavirus; antibody formation; Alfalfa mosaic virus; recombinant proteins; plasmid vectors; gene expression; mice; Nicotiana tabacum; vaccination; vaccine development; viral antigens
Abstract:
... The coat protein (CP) of alfalfa mosaic virus was used as a carrier molecule to express antigenic peptides from rabies virus and HIV. The antigens were separately cloned into the reading frame of alfalfa mosaic virus CP and placed under the control of the subgenomic promoter of tobacco mosaic virus CP in the 30BRz vector. The in vitro transcripts of recombinant virus with sequences encoding the an ...
DOI:
10.1073/pnas.94.11.5784
PubMed:
9159151
PubMed Central:
PMC20857

13. Antimitotic effects of usnic acid on different biological systems

Author:
Cardarelli, M.; Serino, G.; Campanella, L.; Ercole, P.; De Cicco Nardone, F.; Alesiani, O.; Rossiello, F.
Source:
Cellular and molecular life sciences 1997 v.53 no.8 pp. 667-672
ISSN:
1420-682X
Subject:
protoplasts; seed germination; leukemia; humans; cytotoxicity; adenocarcinoma; lichenic acids; mesophyll; antifungal properties; Saccharomyces cerevisiae; cell division; Fusarium fujikuroi; Nicotiana tabacum; cell lines; metabolic inhibitors; lichens
PubMed:
9351470

14. Antisense expression and overexpression of biotin carboxylase in tobacco leaves

Author:
Shintani, D.; Roesler, K.; Shorrosh, B.; Savage, L.; Ohlrogge, J.
Source:
Plant physiology 1997 v.114 no.3 pp. 881-886
ISSN:
0032-0889
Subject:
1-aminocyclopropane-1-carboxylic acid; Nicotiana tabacum; leaves; plastids; fatty acids; biosynthesis; carboxy-lyases; biotin; enzyme activity; protein composition; quantitative analysis; genetic transformation; transgenic plants; antisense DNA; gene expression; genetic variation
Abstract:
... The plastid acetyl-coenzyme A carboxylase (ACCase) catalyzes the first committed step of fatty acid synthesis and in most plants is present as a heteromeric complex of at least four different protein subunits: the biotin carboxylase (BC), the biotin carboxyl carrier protein, and the alpha and beta subunits of the carboxyltransferase. To gain insight into the subunit organization of this heteromeri ...
PubMed:
9232874
PubMed Central:
PMC158375

15. The Arabidopsis MALE STERILITY 2 protein shares similarity with reductases in elongation/condensation complexes

Author:
Aarts, M.G.M.; Hodge, R.; Kalantidis, K.; Florack, D.; Wilson, Z.A.; Mulligan, B.J.; Stiekema, W.J.; Scott, R.; Pereira, A.
Source:
plant journal 1997 v.12 no.3 pp. 615-623
ISSN:
0960-7412
Subject:
fatty acids; flowering; pollen; promoter regions; plant proteins; cell walls; gene expression; Nicotiana tabacum; cell wall components; Arabidopsis thaliana; fatty alcohols; spores; amino acid sequences; histochemistry; transgenic plants; oxidoreductases; reporter genes; beta-glucuronidase
Abstract:
... The Arabidopsis thaliana MALE STERILITY 2 (MS2) gene product is involved in male gametogenesis. The first abnormalities in pollen development of ms2 mutants are seen at the stage in microsporogenesis when microspores are released from tetrads. Expression of the MS2 gene is observed in tapetum of wild-type flowers at, and shortly after, the release of microspores from tetrads. The MS2 promoter cont ...
DOI:
10.1046/j.1365-313X.1997.00615.x
PubMed:
9351246

16. Association of caffeoyl coenzyme A 3-O-methyltransferase expression with lignifying tissues in several dicot plants

Author:
Ye, Z.H.
Source:
Plant physiology 1997 v.115 no.4 pp. 1341-1350
ISSN:
0032-0889
Subject:
developmental stages; lignification; roots; stems; methylation; immunohistochemistry; gene expression; Nicotiana tabacum; protein composition; plant development; Glycine max; Zinnia violacea; phloem; xylem; lignin; Solanum lycopersicum var. lycopersicum; biosynthesis; caffeic acid; Medicago sativa; Forsythia suspensa; coenzyme A; transferases
Abstract:
... Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT) was previously shown to be associated with lignificationin both in vitro tracheary elements (TEs) and organs of zinnia (Zinnia elegans). However, it is not known whether this is a general pattern in dicot plants. To address this question, polyclonal antibodies against zinnia recombinant CCoAOMT fusion protein were raised and used for immunolocali ...
PubMed:
9414548
PubMed Central:
PMC158599

17. Auxin-cytokinin interactions in wild-type and transgenic tobacco

Author:
Eklof, S.; Astot, C.; Blackwell, J.; Moritz, T.; Olsson, O.; Sandberg, G.
Source:
Plant & cell physiology 1997 v.38 no.3 pp. 225-235
ISSN:
0032-0781
Subject:
biosynthesis; transgenic plants; internodes; metabolites; Nicotiana tabacum; cytokinins; indole acetic acid; genetic variation; leaves
Abstract:
... Cytokinins and auxins are important regulators of plant growth and development, but there is incomplete and conflicting evidence that auxins affect cytokinin metabolism and vice versa. We have investigated these interactions in Nicotiana tabacum L. by separate in planta manipulation of levels of the hormones followed by analysis of the induced changes in the metabolism of the other hormone. Cytoki ...
DOI:
10.1093/oxfordjournals.pcp.a029157

18. Auxin-induced epinasty of tobacco leaf tissues. A nonethylene-mediated response

Author:
Keller, C.P.; Van Volkenburgh, E.
Source:
Plant physiology 1997 v.113 no.2 pp. 603-610
ISSN:
0032-0889
Subject:
Nicotiana tabacum; leaves; cell growth; epinasty; dose response; acetic acid; ethylene; biosynthesis; naphthaleneacetic acid; aminooxyacetic acid; indole acetic acid
Abstract:
... Interveinal strips (10 x 1.5 mm) excised from growing tobacco (Nicotiana tabacum L. cv Xanthi) leaves curled >300 degrees when incubated for 20 h in 5 to 500 micromolar alpha-naphthalene acetic acid or 50 to 500 micromolar indole-3-acetic acid. Epinasty was not induced without auxin or by the auxin analog beta-naphthalene acetic acid, and less substantial epinasty was induced in midrib and vein se ...

19. Boron nutrition of cultured tobacco BY-2 cells. II. Characterization of the boron-polysaccharide complex

Author:
Kobayashi, M.; Ohno, K.; Matoh, T.
Source:
Plant & cell physiology 1997 v.38 no.6 pp. 676-683
ISSN:
0032-0781
Subject:
Nicotiana tabacum; cultured cells; cell growth; cell walls; cell membranes; boron; polysaccharides; quantitative analysis; plant nutrition; rhamnose; galacturonic acid; nutrient requirements; nutrient deficiencies; chemical constituents of plants; binding sites
Abstract:
... In cultured tobacco BY-2 cells, more than 90% of the cellular boron (B) occurs in the cell wall and a negligible amount of B is detected in the membrane fraction. Nearly 80% of the cell wall B binds to rhamnogalacturonan II(RG-II) to form a borate-dimeric RG-II complex. Monomeric RG-II is not detected in the cell wall, but it is detected in the extracellular polysaccharides. The complex is reconst ...
DOI:
10.1093/oxfordjournals.pcp.a029220

20. The C-terminal HDEL sequence is sufficient for retention of secretory proteins in the endoplasmic reticulum (ER) but promotes vacuolar targeting of proteins that escape the ER

Author:
Gomord, V.; Denmat, L.A.; Fitchette-Laine, A.C.; Satiat-Jeunemaitre, B.; Hawes, C.; Faye, L.
Source:
plant journal 1997 v.11 no.2 pp. 313-325
ISSN:
0960-7412
Subject:
fractionation; lysosomes; transgenic plants; Agrobacterium radiobacter; plant proteins; vacuoles; protein transport; Nicotiana tabacum; signal peptide; cell suspension culture; cytochemistry; binding sites; protein degradation; immunocytochemistry; endoplasmic reticulum; NADH dehydrogenase; Escherichia coli
Abstract:
... Proteins are co-translationally transferred into the endoplasmic reticulum (ER) and then either retained or transported to different intracellular compartments or to the extracellular space. Various molecular signals necessary for retention in the ER or targeting to different compartments have been identified. In particular, the HDEL and KDEL signals used for retention of proteins in yeast and ani ...
DOI:
10.1046/j.1365-313X.1997.11020313.x
PubMed:
9076996

21. C-terminal deletion mutant of pokeweed antiviral protein inhibits viral infection but does not depurinate host ribosomes

Author:
Tumer, N.E.; Hwang, D.J.; Bonness, M.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1997 v.94 no.8 pp. 3866-3871
ISSN:
0027-8424
Subject:
genetic transformation; antiviral properties; complementary DNA; transgenic plants; Potato virus X; Saccharomyces cerevisiae; ribosomes; binding proteins; protein synthesis inhibitors; gene expression; Nicotiana tabacum; mutants; disease resistance; Phytolacca americana; N-glycoside hydrolases
Abstract:
... Pokeweed antiviral protein (PAP), a 29-kDa protein isolated from Phytolacca americana, inhibits translation by catalytically removing a specific adenine residue from the large rRNA of the 60S subunit of eukaryotic ribosomes. In addition to its ribosome-inactivating ability, PAP has potent antiviral activity against many plant and animal viruses, including HIV. We recently described the isolation a ...
DOI:
10.1073/pnas.94.8.3866
PubMed:
9108070
PubMed Central:
PMC20533

22. Calcium distribution in fertilized and unfertilized ovules and embryo sacs of Nicotiana tabacum L

Author:
Tian, H.Q.; Russell, S.D.
Source:
Planta 1997 v.202 no.1 pp. 93-105
ISSN:
0032-0935
Subject:
Nicotiana tabacum; ovules; pollination; flowering; plant organs; cytochemistry; cytoplasm; chemical precipitation; embryo (plant); pollen tubes; potassium; antimony; inorganic ions; calcium; translocation (plant physiology); cell wall components
Abstract:
... Potassium antimonate was used to localize Ca2+ in tobacco ovules from 0 to 7 d after anthesis in pollinated and emasculated flowers. Antimonate binds "loosely bound" Ca2+ into calcium antimonate; less-soluble forms are unavailable and free calcium usually escapes. Ovules are immature at anthesis. Abundant calcium precipitates in nucellar cells surrounding the micropylar canal. A difference between ...
DOI:
10.1007/s004250050107

23. Cell-cycle-regulated transcription of A- and B-type plant cyclin genes in synchronous cultures

Author:
Ito, M.; Marie-Claire, C.; Sakabe, M.; Ohno, T.; Hata, S.; Kouchi, H.; Hashimoto, J.; Fukuda, H.; Komamine, A.; Watanabe, A.
Source:
plant journal 1997 v.11 no.5 pp. 983-992
ISSN:
0960-7412
Subject:
messenger RNA; Catharanthus roseus; complementary DNA; cell division; amino acid sequences; gene expression; transcription (genetics); cultured cells; Nicotiana tabacum; reporter genes; promoter regions; plant proteins
Abstract:
... Synchronously dividing cell cultures of Catharanthus roseus were used to isolate cDNAs for two mitotic cyclins, named CYS and CYM. The deduced protein sequence of CYS is similar to that of A-type cyclins, and CYM belongs to the group of B-type cyclins. In a fashion similar to the pattern of expression seen for A-type and B-type cyclins in mammalian cells, CYS is expressed before CYM in C. roseus c ...
DOI:
10.1046/j.1365-313X.1997.11050983.x
PubMed:
9193070

24. Cell-to-cell movement of potato spindle tuber viroid

Author:
Ding, B.; Kwon, M.O.; Hammond, R.; Owens, R.
Source:
plant journal 1997 v.12 no.4 pp. 931-936
ISSN:
0960-7412
Subject:
guard cells; plasmodesmata; complementary DNA; Potato spindle tuber viroid; mesophyll; plasmids; Nicotiana tabacum; nucleotide sequences; symplast; RNA; fluorescent dyes; Solanum lycopersicum var. lycopersicum
Abstract:
... Viroids are non-translatable, autonomously replicating circular RNAs that infect only plants. An important component of the viroid infection process is cell-to-cell movement; however, there is virtually no information available about the pathways and mechanisms of this process. In this study, potato spindle tuber viroid (PSTVd) has been used as a model system to investigate the mechanism of viroid ...
DOI:
10.1046/j.1365-313X.1997.12040931.x
PubMed:
9375403

25. Cell-to-cell trafficking of cucumber mosaic virus movement protein:green fluorescent protein fusion produced by biolistic gene bombardment in tobacco

Author:
Itaya, A.; Hickman, H.; Bao, Y.; Nelsen, R.; Ding, B.
Source:
plant journal 1997 v.12 no.5 pp. 1223-1230
ISSN:
0960-7412
Subject:
Nicotiana tabacum; Cucumber mosaic virus; viral proteins; biolistics; transgenic plants; leaves; reporter genes; plasmodesmata; beta-glucuronidase; animal proteins; recombinant proteins; Scyphozoa; translocation (plant physiology)
Abstract:
... Previous micro-injection studies showed that some recombinant viral movement proteins and plant proteins produced in and purified from Escherichia coli could traffic from cell to cell. However, the relevance of these findings obtained by micro-injecting proteins produced in E. coli to the real functions of these proteins when produced in planta has been questioned. In this study, specific gene con ...
DOI:
10.1046/j.1365-313X.1997.12051223.x

26. Changes in the activity and mRNA of cinnamyl alcohol dehydrogenase during tracheary element differentiation in zinnia

Author:
Sato, Y.; Watanabe, T.; Komamine, A.; Hibino, T.; Shibata, D.; Sugiyama, M.; Fukuda, H.
Source:
Plant physiology 1997 v.113 no.2 pp. 425-430
ISSN:
0032-0889
Subject:
Zinnia violacea; mesophyll; lignin; biosynthesis; tracheids; cell differentiation; cinnamic acid; alcohol dehydrogenase; enzyme activity; messenger RNA; gene expression; complementary DNA; Eucalyptus; Nicotiana tabacum
Abstract:
... Changes in the enzymatic activity of cinnamyl alcohol dehydrogenase (CAD) and in the expression of a gene for CAD during tracheary element (TE) differentiation were investigated in cultures of single cells isolated from the mesophyll of zinnia (Zinnia elegans). In cultures in which TE differentiation was induced (TE-inductive cultures), CAD activity increased from h 36 after the start of culture ( ...
PubMed:
9046592
PubMed Central:
PMC158157

27. Characterization and functional expression of a ubiquitously expressed tomato pectin methylesterase

Author:
Gaffe, J.; Tiznado, M.E.; Handa, A.K.
Source:
Plant physiology 1997 v.114 no.4 pp. 1547-1556
ISSN:
0032-0889
Subject:
Solanum lycopersicum var. lycopersicum; Nicotiana tabacum; roots; leaves; protein composition; pectinesterase; enzyme activity; hypocotyls; epicotyls; cotyledons; developmental stages; protein synthesis; genes; gene expression; amino acid sequences; messenger RNA; transgenic plants; genetic code; cell wall components; transcription (genetics)
Abstract:
... Pectin methylesterase (PME), a ubiquitous enzyme in plants, de-esterifies the methoxylated pectin in the plant cell wall. We have characterized a PME gene (designated as pmeu1) from tomato (Lycopersicon esculentum) with an expression that is higher in younger root, leaf, and fruit tissues than in older tissues. Hypocotyls and epicotyls show higher accumulation of pmeu1 transcripts compared with co ...
PubMed:
9276962
PubMed Central:
PMC158449

28. Characterization and purification of herbicide naproanilide receptor from tobacco mesophyll callus

Author:
Wang, Y.S.; Hwang, K.L.; Hsieh, Y.N.
Source:
Journal of agricultural and food chemistry 1997 v.45 no.7 pp. 2723-2727
ISSN:
0021-8561
Subject:
receptors; Nicotiana tabacum; plant tissues; anilide herbicides
Abstract:
... Herbicide naproanilide receptor proteins in tobacco mesophyll callus were isolated and characterized. The reaction between naproanilide and its receptor proteins is a specific binding with dissociation constant Kd = 1.8 x 10(-7) M. The optimum conditions for the binding reaction are 0-4 degrees c at pH 5.8-7.8 for 30-45 min. Purification of naproanilide receptor proteins on a chromatograph with Se ...
DOI:
10.1021/jf960590d

29. Characterization of RNA-mediated resistance to tomato spotted wilt virus in transgenic tobacco plants expressing NSM gene sequences

Author:
Marcel Prins; Marjolein Kikkert; Cahya Ismayadi; William de Graauw; Peter de Haan; Rob Goldbach
Source:
Plant molecular biology 1997 v.33 no.2 pp. 235-243
ISSN:
0167-4412
Subject:
messenger RNA; antisense RNA; transgenic plants; gene expression; resistance mechanisms; Nicotiana tabacum; disease resistance; genes; nucleotide sequences; progeny; viral proteins; viruses; Tomato spotted wilt orthotospovirus
Abstract:
... Transgenic Nicotiana tabacum plants expressing RNA sequences of the tomato spotted wilt virus NSM gene, which encodes the putative viral movement protein, were found to be highly resistant to infection with the virus. Expression of untranslatable as well as anti-sense RNA of the NSM gene resulted in resistance levels as high as those in plants expressing translatable RNA sequences. For all three t ...
DOI:
10.1023/A:1005729808191

30. Characterization of a DNA-binding protein that interacts with 5' flanking regions of two fruit-ripening genes

Author:
Coupe, S.A.; Deikman, J.
Source:
plant journal 1997 v.11 no.6 pp. 1207-1218
ISSN:
0960-7412
Subject:
transcription factors; ethylene; messenger RNA; complementary DNA; plasmid vectors; amino acid sequences; gene expression; Nicotiana tabacum; ripening; DNA-binding proteins; genes; binding sites; promoter regions; Solanum lycopersicum var. lycopersicum
Abstract:
... The E4/E8 binding protein (E4/E8BP) interacts with sequences in the 5' flanking regions of two genes, E4 and E8, that are coordinately regulated by ethylene during tomato fruit ripening. The DNA-binding activity of this protein increases during fruit ripening, and it may play a role in regulation of these genes. To begin to understand the function of this protein, a cDNA has been isolated that enc ...
DOI:
10.1046/j.1365-313X.1997.11061207.x
PubMed:
9225464

31. Characterization of a diffusible signal capable of inducing defense gene expression in tobacco

Author:
Chappell, J.; Levine, A.; Tenhaken, R.; Lusso, M.; Lamb, C.
Source:
Plant physiology 1997 v.113 no.2 pp. 621-629
ISSN:
0032-0889
Subject:
Nicotiana tabacum; cell suspension culture; Phytophthora cryptogea; plant pathogenic fungi; pathogenicity; disease resistance; genes; phytoalexins; biosynthesis; lyases; enzyme activity; measurement; gene expression; Glycine max; hydrogen peroxide; pathogenesis-related proteins; sesquiterpenoids
Abstract:
... Treatment of tobacco (Nicotiana tabacum) cell-suspension cultures with cryptogein, an elicitin protein from Phytophthora cryptogea, resulted in the release of a factor(s) that diffused through a 1000-D cutoff dialysis membrane and was capable of inducing sesquiterpene cyclase enzyme activity (a key phytoalexin biosynthetic enzyme in solanaceous plants) when added to fresh cell-suspension cultures. ...

32. Characterization of a tobacco extensin gene and regulation of its gene family in healthy plants and under various stress conditions

Author:
Cathy Hirsinger; Yves Parmentier; Andrée Durr; Jacqueline Fleck; Elisabeth Jamet
Source:
Plant molecular biology 1997 v.33 no.2 pp. 279-289
ISSN:
0167-4412
Subject:
DNA; Nicotiana sylvestris; Nicotiana tabacum; Nicotiana tomentosiformis; cell suspension culture; clones; crosslinking; extensin; gene expression regulation; genomic libraries; genomics; leaves; messenger RNA; molecular cloning; multigene family; plant damage; plant ovary; roots; seed germination; seeds; stems; tobacco
Abstract:
... A genomic clone (Ext 1.4) encoding an extensin was isolated from a Nicotiana tabacum genomic library. The encoded polypeptide showed features characteristic of extensins such as Ser-(Pro)4 repeats and a high content in Tyr and Lys residues. The presence of one Tyr-Leu-Tyr-Lys motif suggests the possibility for one intramolecular isodityrosine cross-link whereas numerous Val-Tyr-Lys motifs may part ...
DOI:
10.1023/A:1005738815383

33. Characterization of antisense transformed plants deficient in the tobacco anionic peroxidase

Author:
Lagrimini, L.M.; Gingas, V.; Finger, F.; Rothstein, S.; Liu, T.T.Y.
Source:
Plant physiology 1997 v.114 no.4 pp. 1187-1196
ISSN:
0032-0889
Subject:
cell growth; chemical constituents of plants; Nicotiana sylvestris; developmental stages; lignification; genes; roots; antisense RNA; phenotype; stems; cell walls; Nicotiana tabacum; genotype; peroxidase; leaves; defense mechanisms; genetic code; morphogenesis; transcription (genetics); xylem; lignin; enzyme activity; isozymes; transgenic plants; stress response; genetic variation; plant morphology
Abstract:
... On the basis of the biological compoundsthat they metabolize, plant peroxidases have long been implicated in plant growth, cell wall biogenesis, lignification, and host defenses. Transgenic tobacco (Nicotiana tabacum L.) plants that underexpress anionic peroxidase were generated using antisense RNA. The antisense RNA was found to be specific for the anionic isoenzyme and highly effective, reducing ...

34. Characterization of regions within the n-terminal 6-kilodalton domain of phytochrome A that modulate its biological activity

Author:
Jordan, E.T.; Marita, J.M.; Clough, R.C.; Vierstra, R.D.
Source:
Plant physiology 1997 v.115 no.2 pp. 693-704
ISSN:
0032-0889
Subject:
Avena sativa; seedlings; photoreceptors; red light; far-red light; phytochrome; apoproteins; plant development; alanine; serine; amino acid sequences; gene transfer; transgenic plants; Nicotiana tabacum; gene expression; genetic variation; light harvesting complex
Abstract:
... Phytochrome A (phyA) is a red/far-red (FR) light photoreceptor responsible for initiating numerous light-mediated plant growth and developmental responses, especially in FR light-enriched environments. We previously showed that the first 70 amino acids of the polypeptide contain at least two regions with potentially opposite functions (E.T. Jordan, J.R. Cherry, J.M. Walker, R.D. Vierstra [1996] Pl ...
PubMed:
9342873
PubMed Central:
PMC158530

35. Characterization of the targeted nuclear accumulation of GFP within the cells of transgenic plants

Author:
Grebenok, R.J.; Lambert, G.M.; Galbraith, D.W.
Source:
plant journal 1997 v.12 no.3 pp. 685-696
ISSN:
0960-7412
Subject:
Nicotiana tabacum; transgenic plants; cytoplasm; active transport; molecular weight; amino acid sequences; plant proteins; cytochemistry; spatial distribution; leaves; beta-glucuronidase; enzyme activity; animal proteins; Scyphozoa; green fluorescent protein; Aequorea victoria
Abstract:
... The soluble proteins of the nucleoplasm are synthesized on cytoplasmic ribosomes. Proteins larger than about 40 kDa are post-translationally targeted to the nucleus via energy-dependent processes, passing through the nuclear pore complex into the nucleoplasm. Targeting involves nuclear localization signals (NLSs) found within the primary sequences of the imported proteins. In higher plants, inform ...
DOI:
10.1046/j.1365-313X.1997.00685.x

36. Clavibacter michiganensis subsp. sepedonicus elicits a hypersensitive response in tobacco and secretes hypersensitive response-inducing protein(s)

Author:
Nissinen, R.; Lai, F.M.; Laine, M.J.; Bauer, P.J.; Reilley, A.A.; Li, X.; De Boer, S.H.; Ishimaru, C.A.; Metzler, M.C.
Source:
Phytopathology 1997 v.87 no.7 pp. 678-684
ISSN:
0031-949X
Subject:
plant pathogenic bacteria; protein synthesis; hypersensitivity; Clavibacter michiganensis subsp. sepedonicus; necrosis; strain differences; Nicotiana tabacum; virulence; Solanum lycopersicum var. lycopersicum
Abstract:
... Strains of Clavibacter michiganenis subsp. sepedonicus, causal agent of bacterial ring rot of potato, showed marked differences in virulence on host plants. When infiltrated into tobacco leaves, virulent strains caused a rapid localized necrotic response (within 24 to 48 h) characteristic of the hypersensitive response (HR), whereas nonpathogenic strains did not. Concentrated cell-free culture sup ...
DOI:
10.1094/PHYTO.1997.87.7.678
PubMed:
18945088

37. Cloning and characterization of a cDNA encoding an elicitor of Phytophthora parasitica var. nicotianae that shows cellulose-binding and lectin-like activities

Author:
Mateos, F.V.; Richauer, M.; Esquerre-Tugaye, M.T.
Source:
Molecular plant-microbe interactions 1997 v.10 no.9 pp. 1045-1053
ISSN:
0894-0282
Subject:
Phytophthora nicotianae var. parasitica; complementary DNA; cellulose; lectins; binding proteins; glycoproteins; amino acid sequences; nucleotide sequences; cysteine; hydrolysis; hemagglutination; Nicotiana tabacum; leaves; necrosis; gene expression; defense mechanisms; virulence; disease resistance; cell wall components
Abstract:
... Phytophthora parasitica var. nicotianae produces a 34-kDa glycoprotein elicitor (CBEL) that is localized in the cell wall. A cDNA encoding (he protein moiety of this elicitor was cloned and characterized. The deduced amino acid sequence consisted of two direct repeats of a cysteine-rich domain, joined by a Thr/pro-rich region. Although having no general homology with published sequences, the posit ...
DOI:
10.1094/MPMI.1997.10.9.1045
PubMed:
9390419

38. Cloning and characterization of a plastidal and a mitochondrial isoform of tobacco protoporphyrinogen IX oxidase

Author:
Lermontova, I.; Kruse, E.; Mock, H.P.; Grimm, B.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1997 v.94 no.16 pp. 8895-8900
ISSN:
0027-8424
Subject:
Nicotiana tabacum; complementary DNA; oxidoreductases; isozymes; mitochondria; chloroplasts; nucleotide sequences; amino acid sequences; immunocytochemistry; gene expression; messenger RNA; plant development; enzyme activity; protein transport; leaves; roots
Abstract:
... Protoporphyrinogen IX oxidase is the last enzyme in the common pathway of heme and chlorophyll synthesis and provides precursor for the mitochondrial and plastidic heme synthesis and the predominant chlorophyll synthesis in plastids. We cloned two different, full-length tobacco cDNA sequences by complementation of the protoporphyrin-IX-accumulating Escherichia coli hemG mutant from heme auxotrophy ...
DOI:
10.1073/pnas.94.16.8895
PubMed:
9238074
PubMed Central:
PMC23187

39. Composition of photosystem II antenna in light-harvesting complex II antisense tobacco plants at varying irradiances

Author:
Flachman, R.
Source:
Plant physiology 1997 v.113 no.3 pp. 787-794
ISSN:
0032-0889
Subject:
Nicotiana tabacum; thylakoids; photosystem I; photosystem II; light intensity; genes; genetic code; quantitative analysis; chemical constituents of plants; light harvesting complex; transcription (genetics); phenotype
Abstract:
... Plants with genes coding for chlorophyll a/b-binding proteins of light-harvesting complex II (LHCII) in antisense orientation (Lhcb) that are characterized by severely reduced Lhcb transcript levels (below 10% of wild type) do not show a bleached phenotype due to a specific loss of the polypeptide. To produce such a phenotype, a conceptually different antisense approach was tested with a dual-func ...
PubMed:
9085572
PubMed Central:
PMC158197

40. Compromising early salicylic acid accumulation delays the hypersensitive response and increases viral dispersal during lesion establishment in TMV-infected tobacco

Author:
Mur, L.A.J.; Bi, Y.M.; Darby, R.M.; Firek, S.; Draper, J.
Source:
plant journal 1997 v.12 no.5 pp. 1113-1126
ISSN:
0960-7412
Subject:
Nicotiana tabacum; Tobacco mosaic virus; salicylic acid; defense mechanisms; gene expression; oxygenases; transgenic plants; necrosis; virulence; messenger RNA; beta-glucuronidase; reporter genes; pathogenesis; translocation (plant physiology); promoter regions
Abstract:
... To investigate the role of salicylic acid (SA) in the hypersensitive response (HR) its accumulation was compromised during different phases of lesion development by differential expression of a salicylate hydroxylase gene (SH-L). Constitutive suppression of SA accumulation was achieved by expression of a gene fusion between the CaMV35S promoter (35S) and SH-L. Using the H2O2-responsive AoPR1 promo ...
DOI:
10.1046/j.1365-313X.1997.12051113.x
PubMed:
9418052

41. Conditional transgenic expression of the ipt gene indicates a function for cytokinins in paracrine signaling in whole tobacco plants

Author:
Faiss, M.; Zalubilova, J.; Strnad, M.; Schmulling, T.
Source:
plant journal 1997 v.12 no.2 pp. 401-415
ISSN:
0960-7412
Subject:
buds; transgenic plants; meristems; zeatin; dihydrozeatin; transpiration; biochemical pathways; gene expression; glucosides; cytokinins; genes; Nicotiana tabacum; alkyl (aryl) transferases; roots; promoter regions; leaves; shoots
Abstract:
... This study investigated whether an increased production of the plant hormone cytokinin in roots, the main site of its synthesis and putative signaling organ, can influence developmental events, such as growth of axillary shoot meristems or leaf senescence, in the plant shoot. To this end, transgenic tobacco plants (Nicotiana tabacum L.) were generated that conditionally overproduce cytokinins. The ...
DOI:
10.1046/j.1365-313X.1997.12020401.x
PubMed:
9301091

42. Detectable beta-glucuronidase activity in transgenic cranberry is affected by endogenous inhibitors and plant development

Author:
Serres, R.; McCown, B.; Zeldin, E.
Source:
Plant cell reports 1997 v.16 no.9 pp. 641-646
ISSN:
0721-7714
Subject:
Vaccinium macrocarpon; Nicotiana tabacum; leaves; genetic transformation; transgenic plants; gene transfer; beta-glucuronidase; enzyme activity; enzyme inhibitors; polyvidone; flavonols; anthocyanidins; plant extracts; histochemistry; reporter genes; gene expression; proanthocyanidins
Abstract:
... Extracts of cranberry, rich in flavonols and proanthocyanins, inactivated beta-glucuronidase (GUS) in assays using either purified bacterial GUS or preparations of transgenic tobacco (Nicotiana tabacum L.) or transgenic cranberry (Vaccinium macrocarpon Ait.) expressing the gusA gene. Histochemical GUS assays produced random and generally unpredictable staining. The addition of polyvinylpolypyrroli ...
DOI:
10.1007/BF01275507

43. Development of a binary vector system for plant transformation based on the supervirulent Agrobacterium tumefaciens strain Chry5

Author:
Torisky, R.S.; Kovacs, L.; Avdiushko, S.; Newman, J.D.; Hunt, A.G.; Collins, G.B.
Source:
Plant cell reports 1997 v.17 no.2 pp. 102-108
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; Glycine max; transgenic plants; Agrobacterium radiobacter; strain differences; virulence; plasmids; chromosome mapping; Nicotiana tabacum; genetic vectors; genetic markers
Abstract:
... This report describes the disarming of Agrobacterium tumefaciens Chry5, a strain highly tumorigenic on soybean. Disarming was achieved by removing an approximately 16.5-kb segment of the 285-kb Ti plasmid pTiChry5, including approximately 4 kb of the oncogenic T-DNA and an extended region right of the T-DNA, and replacing it with a gene for carbenicillin resistance, through homologous recombinatio ...
DOI:
10.1007/s002990050360

44. Development of necrosis and activation of disease resistance in transgenic tobacco plants with severely reduced catalase levels

Author:
Takahashi, H.; Chen, Z.; Du, H.; Liu, Y.; Klessig, D.F.
Source:
plant journal 1997 v.11 no.5 pp. 993-1005
ISSN:
0960-7412
Subject:
Nicotiana tabacum; transgenic plants; catalase; disease resistance; necrosis; enzyme activity; salicylic acid; binding proteins; antisense RNA; protein synthesis; chlorosis; Tobacco mosaic virus; nucleotide sequences; amino acid sequences; gene expression; pathogenesis-related proteins; chemical constituents of plants
Abstract:
... Numerous studies argue that salicylic acid (SA) is an important component of the plant signal transduction pathway(s) leading to disease resistance. The discovery that the SA-binding protein is a catalase, whose activity is blocked by SA, led to the proposal that one of SA's modes of action is to inhibit this H2O2-degrading enzyme and thus elevate H2O2 levels. To test this model, an attempt was ma ...
DOI:
10.1046/j.1365-313X.1997.11050993.x
PubMed:
9193071

45. Developmental and environmental regulation of tissue- and cell-specific expression for a pea protein farnesyltransferase gene in transgenic plants

Author:
Zhou, D.; Qian, D.; Cramer, C.L.; Yang, Z.
Source:
plant journal 1997 v.12 no.4 pp. 921-930
ISSN:
0960-7412
Subject:
root tips; root primordia; promoter regions; hypocotyls; stems; gene expression; Nicotiana tabacum; petioles; cytochemistry; apical meristems; phloem; enzyme activity; histochemistry; seed germination; root systems; transgenic plants; cotyledons; reporter genes; cell cycle; transferases; beta-glucuronidase
Abstract:
... Farnesylation mediates membrane targeting and in viva activities of several key regulatory proteins such as Ras and Ras-related GTPases and protein kinases in yeast and mammals, and is implicated in cell cycle control and abscisic acid (ABA) signaling in plants. In this study, the developmental expression of a pea protein farnesyl- transferase (FTase) gene was examined using transgenic expression ...
DOI:
10.1046/j.1365-313X.1997.12040921.x
PubMed:
9375402

46. Developmental changes in DNA methylation of the two tobacco pollen nuclei during maturation

Author:
Oakeley, E.J.; Podesta, A.; Jost, J.P.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1997 v.94 no.21 pp. 11721-11725
ISSN:
0027-8424
Subject:
Nicotiana tabacum; pollen; DNA methylation; gametogenesis; cytosine; monoclonal antibodies
Abstract:
... Changes in DNA methylation during tobacco pollen development have been studied by confocal fluorescence microscopy using a monoclonal anti-5-methylcytosine (anti-m5C) antibody and a polyclonal anti-histone H1 (anti-histone) antibody as an internal standard. The specificity of the anti-m5C antibody was demonstrated by a titration series against both single-stranded DNA and double-stranded DNA subst ...
DOI:
10.1073/pnas.94.21.11721
PubMed:
9326677
PubMed Central:
PMC23612

47. Differential accumulation of salicylic acid and salicylic acid-sensitive catalase in different rice tissues

Author:
Chen, Z.; Iyer, S.; Caplan, A.; Klessig, D.F.; Fan, B.
Source:
Plant physiology 1997 v.114 no.1 pp. 193-201
ISSN:
0032-0889
Subject:
Oryza sativa; Nicotiana tabacum; shoots; roots; cell suspension culture; leaves; salicylic acid; biosynthesis; quantitative analysis; catalase; enzyme activity; genes; gene expression; genetic code; chemical constituents of plants
Abstract:
... We previously proposed that salicylic acid (SA)-sensitive catalases serve as biological targets of SA in plant defense responses. To further examine the role of SA-sensitive catalases, we have analyzed the relationship between SA levels and SA sensitivity of catalases in different rice (Oryza sativa) tissues. We show here that, whereas rice shoots contain extremely high levels of free SA, as previ ...

48. Differential activation of two ACC oxidase gene promoters from melon during plant development and in response to pathogen attack

Author:
Lasserre, E.; Godard, F.; Bouquin, T.; Hernandez, J.A.; Pech, J.C.; Roby, D.; Balague, C.
Source:
MGG 1997 v.256 no.3 pp. 211-222
ISSN:
0026-8925
Subject:
ethylene; recombinant DNA; Ralstonia solanacearum; 2,4-D; promoter regions; gene expression; Nicotiana tabacum; mechanical damage; Pseudomonadaceae; oxygenases; infection; genetic recombination; copper sulfate; defense mechanisms; plant development; Cucumis melo; abiotic stress; histochemistry; transgenic plants; reporter genes; abscisic acid; beta-glucuronidase
DOI:
10.1007/s004380050563
PubMed:
9393445

49. Differential expression of CuZn- and Fe-superoxide dismutase genes of tobacco during development, oxidative stress, and hormonal treatments

Author:
Kurepa, J.; Herouart, D.; Montagu, M. van.; Inze, D.
Source:
Plant & cell physiology 1997 v.38 no.4 pp. 463-470
ISSN:
0032-0781
Subject:
Nicotiana tabacum; plant development; oxidation; chloroplasts; gene expression; norflurazon; paraquat; copper; zinc; iron; superoxide dismutase; enzyme activity; genes; stress response
Abstract:
... Chloroplasts of Nicotiana tabacum have two superoxide dismutases: a Fe- and a CuZn-containing enzyme, encoded by the nuclear genes sodB and sodCp, respectively. As a first step in studying the physiological function of these two enzymes, we compared the expression of sodB and sodCp in different plant organs, in response to hormonal treatments, and upon treatment with paraquat and Norflurazon. The ...
DOI:
10.1093/oxfordjournals.pcp.a029190
PubMed:
9177032

50. Differential gene expression in response to auxin treatment in the wild type and rac, an adventitious rooting-incompetent mutant of tobacco

Author:
Lund, S.T.; Smith, A.G.; Hackett, W.P.
Source:
Plant physiology 1997 v.114 no.4 pp. 1197-1206
ISSN:
0032-0889
Subject:
cortex; messenger RNA; cell growth; transgenic plants; developmental stages; cell division; adventitious roots; gene expression; phloem; rooting; genes; Nicotiana tabacum; mutants; auxins; genotype; plant cuttings; histochemistry; callus
Abstract:
... Histological analyses of auxin-treated cuttings from the wildtype and the rac mutant of tobacco (Nicotiana tabacum cv Xanthii) previously revealed that some rac phloem parenchyma or inner cortical parenchyma cells form callus in response to exogenous auxin treatment but these cells never undergo the organized divisions associated with adventitious root initiation in the wild type. Here we report t ...

51. Directed overexpression of PHYA locally suppresses stem elongation and leaf senescence responses to far-red radiation

Author:
Rousseaux, M.C.; Ballare, C.L.; Jordan, E.T.; Vierstra, R.D.
Source:
Plant, cell and environment 1997 v.20 no.12 pp. 1551-1558
ISSN:
0140-7791
Subject:
nitrate reductase; genes; promoter regions; shoots; far-red light; gene expression; Nicotiana tabacum; phytochrome; leaves; Arabidopsis thaliana; weight; complementary DNA; morphogenesis; ubiquitin; enzyme activity; canopy; transgenic plants; internodes; plant morphology; chemical constituents of plants; chlorophyll
Abstract:
... Ectopic overexpression of an oat PHYA cDNA in tobacco under the cauliflower mosaic virus 35S promoter results in plants with reduced morphological responses to far-red radiation (FR). We have tested the hypothesis that it is possible to molecularly 'mask' stems and leaves to FR-induced elongation and senescence responses by targeting the overexpression of PHYA with appropriate promoters. Oat PHYA ...
DOI:
10.1046/j.1365-3040.1997.d01-51.x

52. Distribution profiles of dry matter and total nitrogen in leaves and stems of oriental field-grown tobacco plants at different nitrogen levels

Author:
Drossopoulos, J.B.; Bouranis, D.L.; Aivalakis, G.; Tsomokos, A.
Source:
Journal of plant nutrition 1997 v.20 no.6 pp. 695-713
ISSN:
0190-4167
Subject:
weight; nitrogen content; stems; nutrient availability; Nicotiana tabacum; dry matter partitioning; application rate; ammonium nitrate; leaves
Abstract:
... Oriental tobacco plants (Nicotiana tabacum L. cv Myrodata Agrinion) were grown without nitrogen (N) fertilization (N0) and with added ammonium nitrate at a rate of 50 kg. ha-1 (N1) and 100 kg.ha-1 (N2). Non-uniform patterns for leaf FW and DW changes per node showed a decreasing trend from lower to upper nodes during the vegetative stage. From approaching flowering to fruit set, these patterns bec ...
DOI:
10.1080/01904169709365287

53. Diverse amino acid residues function within the type 1 peroxisomal targeting signal. Implications for the role of accessory residues upstream of the type 1 peroxisomal targeting signal

Author:
Mullen, R.T.; Lee, M.S.; Flynn, C.R.; Trelease, R.N.
Source:
Plant physiology 1997 v.115 no.3 pp. 881-889
ISSN:
0032-0889
Subject:
lysine; leucine; peroxisomes; serine; glyoxylate cycle; cytosol; binding proteins; translocation (plant physiology); Nicotiana tabacum; nucleotide sequences; chloramphenicol acetyltransferase; glyoxysomes; cell suspension culture
Abstract:
... The purpose of this study was to determine whether the plant type 1 peroxisomal targeting signal (PTS1) utilizes amino acid residues that do not strictly adhere to the serine-lysine-leucine (SKL) motif (small-basic-hydrophobic residues). Selected residues were appended to the C terminus of chloramphenicol acetyltransferase (CAT) and were tested for their ability to target CAT fusion proteins to gl ...
PubMed:
9390426
PubMed Central:
PMC158551

54. Do plant phenolics confer resistance to specialist and generalist insect herbivores?

Author:
Bi, J.L.; Felton, G.W.; Murphy, J.B.; Howles, P.A.; Dixon, R.A.; Lamb, C.J.
Source:
Journal of agricultural and food chemistry 1997 v.45 no.11 pp. 4500-4504
ISSN:
0021-8561
Subject:
Manduca sexta; Heliothis virescens; pest resistance; phenolic compounds; phenylalanine ammonia-lyase; Nicotiana tabacum; chemical constituents of plants
Abstract:
... The impact of phenolics on a specialist herbivore, Manduca sexta, and a generalist herbivore, Heliothis virescens, was investigated using transgenic tobacco with differential expression of phenylalanine ammonia-lyase. Foliar phenolics such as chlorogenic acid, rutin, and total flavonoids differentially accumulated in the respective transgenic tobacco lines; the amount of chlorogenic acid ranged fr ...
DOI:
10.1021/jf970555m

55. Dual effect of phytochrome A on hypocotyl growth under continuous red light

Author:
Mazzella, M.A.; Magliano, T.M.A.; Casal, J.J.
Source:
Plant, cell and environment 1997 v.20 no.2 pp. 261-267
ISSN:
0140-7791
Subject:
Arabidopsis thaliana; Avena sativa; Nicotiana tabacum; transgenic plants; phytochrome; red light; hypocotyls; length; seedling growth; photoperiod; light intensity; temporal variation; mutants; far-red light
Abstract:
... The role of phytochrome A in the control of hypocotyl growth under continuous red light (Rc) was investigated using phyA and phyB mutants of Arabidopsis thaliana, which lack phytochrome A (phyA) or phytochrome B (phyB), respectively, and transgenic seedlings of Nicotiana tabacum overexpressing Avena phyA, compared to the corresponding wild type (WT). In WT seedlings of A. thaliana, hypocotyl growt ...
DOI:
10.1046/j.1365-3040.1997.d01-62.x

56. Ectopic expression of a tobacco homeobox gene, NTH15, dramatically alters leaf morphology and hormone levels in transgenic tobacco

Author:
Tamaoki, M.; Kusaba, S.; Kano-Murakami, Y.; Matsuoka, M.
Source:
Plant & cell physiology 1997 v.38 no.8 pp. 917-927
ISSN:
0032-0781
Subject:
chemical constituents of plants; developmental stages; genes; abnormal development; shoots; phenotype; gibberellic acid; gene expression; Nicotiana tabacum; leaves; apical meristems; quantitative analysis; complementary DNA; morphogenesis; amino acid sequences; indole acetic acid; gene transfer; transgenic plants; zeatin; flowers; nucleotide sequences; abscisic acid; plant morphology
Abstract:
... The shoot apical meristem functions to generate the lateral organs of a plant throughout the vegetative and reproductive phases. Homeobox genes play key roles in controlling such developmental programs, but their modes of action have not been well defined. Here we describe isolation and biological functions of a novel tobacco homeobox gene, designated NTH15 (Nicotiana tabacum homeobox 15), from a ...
DOI:
10.1093/oxfordjournals.pcp.a029252
PubMed:
9327591

57. Effect of an entomopathogen on adaptation of Heliothis virescens populations to transgenic host plants

Author:
Johnson, M.T.; Gould, F.; Kennedy, G.G.
Source:
Entomologia experimentalis et applicata 1997 v.83 no.2 pp. 121-135
ISSN:
0013-8703
Subject:
transgenic plants; tritrophic interactions; Nomuraea rileyi; Bacillus thuringiensis; Nicotiana tabacum; Heliothis virescens; endotoxins; pest resistance; mortality; artificial selection
DOI:
10.1046/j.1570-7458.1997.00165.x

58. Effect of cadimum treatment on the expression of chimeric genes in transgenic tobacco seedlings and calli

Author:
Stefanov, I.; Frank, J.; Gedamu, L.; Misra, S.
Source:
Plant cell reports 1997 v.16 no.5 pp. 291-294
ISSN:
0721-7714
Subject:
Nicotiana tabacum; cadmium; dose response; phytotoxicity; plant breeding; chromosome mapping; gene transfer; transgenic plants; chimerism; reporter genes; gene expression; seedlings; callus; beta-glucuronidase; enzyme activity; measurement; glucuronic acid; transcription (genetics); promoter regions
Abstract:
... Transgenic tobacco plants and calli bearing the bacterial uidA A gene under the transcriptional control of rbcS, mas and CaMV35S promoter(s) were exposed to different concentrations of cadmium. The transcriptional activity of the promoters was monitored using p-nitrophenyl beta-D-glucuronide as a substrate for the beta-glucuronidase (uidA) reporter enzyme. The rbcS promoter was repressed by high c ...
DOI:
10.1007/BF01088283

59. Effectiveness of the bacterial gene codA encoding cytosine deaminase as a negative selectable marker in Agrobacterium-mediated plant transformation

Author:
Schlaman, H.R.M.; Hooykaas, P.J.J.
Source:
plant journal 1997 v.11 no.6 pp. 1377-1385
ISSN:
0960-7412
Subject:
amidine hydrolases; genetic transformation; protoplasts; plant development; callus; kanamycin; transgenic plants; Agrobacterium radiobacter; seedlings; plasmid vectors; explants; flucytosine; Nicotiana tabacum; leaves; culture media; roots; genetic markers; shoots
Abstract:
... The usefulness of the E. coli codA gene encoding cytosine deaminase as a conditional toxic gene was explored during various stages of plant development and in different Agrobacterium-mediated transformation protocols. To this end, several independent tobacco lines transgenic for codA were isolated and these were tested for their sensitivity to 5-fluorocytosine (5-FC) at different developmental sta ...
DOI:
10.1046/j.1365-313X.1997.11061377.x

60. Effects of leaf surface moisture and relative humidity on the efficacy of sugar esters from Nicotiana gossei against the tobacco aphid (Homoptera: Aphididae)

Author:
Xia, Y.; Johnson, A.W.
Source:
Journal of economic entomology 1997 v.90 no.4 pp. 1010-1014
ISSN:
0022-0493
Subject:
relative humidity; sucrose esters; botanical insecticides; Myzus persicae nicotianae; water content; Nicotiana tabacum; toxicity; insect control; spraying; mortality; leaves; South Carolina
Abstract:
... The effects of leaf surface moisture and relative humidity on the toxicity of sugar esters extracted from Nicotiana gossei Domin to the tobacco aphid, Myzus nicotianae Blackman, were examined in the laboratory and the field. The efficacy of sugar esters applied at a concentration of 1 mg/ml against tobacco aphids was >5 times higher under moist conditions (either wet leaf surface or wet filter pap ...
DOI:
10.1093/jee/90.4.1010

61. Effects of natural enemies on relative fitness of Heliothis virescens genotypes adapted and not adapted to resistant host plants

Author:
Johnson, M.T.; Gould, F.; Kennedy, G.G.
Source:
Entomologia experimentalis et applicata 1997 v.82 no.2 pp. 219-230
ISSN:
0013-8703
Subject:
parasitism; parasites; transgenic plants; spatial distribution; biological control agents; natural enemies; tritrophic interactions; nutrition; Nomuraea rileyi; Bacillus thuringiensis; Nicotiana tabacum; genotype; larvae; Heliothis virescens; Campoletis sonorensis; endotoxins; pest resistance; mortality
DOI:
10.1046/j.1570-7458.1997.00133.x

62. Efficient in vitro transcription of plant nuclear tRNASer genes in a nuclear extract from tobacco cultured cells

Author:
Yukawa, Y.; Sugita, M.; Sugiura, M.
Source:
plant journal 1997 v.12 no.4 pp. 965-970
ISSN:
0960-7412
Subject:
Nicotiana tabacum; cell suspension culture; genes; plant extracts; transfer RNA; DNA-directed RNA polymerase; transcription (genetics)
Abstract:
... A nuclear extract derived from tobacco cultured BY-2 cells supports RNA polymerase III-dependent transcription of Arabidopsis tRNA(Ser) genes. Primer extension analysis indicated that the transcription starts at 6 bp upstream from the 5' end of tRNA coding region. Procedures for nuclear extraction and in vitro reaction conditions have been optimized for tRNA transcription, which allows direct dete ...
DOI:
10.1046/j.1365-313X.1997.12040965.x
PubMed:
9375407

63. Elevated CO2 effects during leaf ontogeny: a new perspective on acclimation

Author:
Miller, A.; Tsai, C.H.; Hemphill, D.; Endres, M.; Rodermel, S.; Spalding, M.
Source:
Plant physiology 1997 v.115 no.3 pp. 1195-1200
ISSN:
0032-0889
Subject:
ribulose-bisphosphate carboxylase; carbon dioxide; developmental stages; net assimilation rate; acclimation; ontogeny; Nicotiana tabacum; photosynthesis; enzyme activity; leaves
Abstract:
... For many plants growth in elevated CO2 leads to reduced rates of photosynthesis. To examine the role that leaf ontogeny plays in the acclimation response, we monitored photosynthesis and some related parameters at short intervals throughout the ontogenetic development of tobacco (Nicotiana tabacum L.) leaves under ambient (350 microliters L-1)- and high (950 microliters L-1)-CO2 conditions. The pa ...

64. Encapsidation of potyviral RNA in various forms of transgene coat protein is not correlated with resistance in transgenic plants

Author:
Hammond, J.; Dienelt, M.M.
Source:
Molecular plant-microbe interactions 1997 v.10 no.8 pp. 1023-1027
ISSN:
0894-0282
Subject:
plant viruses; Nicotiana glutinosa; coat proteins; phenotype; transgenic plants; Nicotiana benthamiana; recombinant proteins; Nicotiana tabacum; disease resistance; Bean yellow mosaic virus; genes; RNA; virus replication
Abstract:
... Transgenic plants expressing either bean yellow mosaic potyvirus or chimeric potyvirus coat protein (CP) were inoculated with various potyviruses. Antigen-coated plate, indirect enzyme-linked immunosorbent assay and immunnoelectron microscopy of virus purified from transgenic plants showed that progeny virions contained from <1% to as much as 25% transgenic CP. Different levels of transcapsidation ...
DOI:
10.1094/MPMI.1997.10.8.1023
PubMed:
9353947

65. Enhanced tolerance to bacterial pathogens caused by the transgenic expression of barley lipid transfer protein LTP2

Author:
Molina, A.; Garcia-Olmedo, F.
Source:
plant journal 1997 v.12 no.3 pp. 669-675
ISSN:
0960-7412
Subject:
Nicotiana tabacum; Arabidopsis thaliana; Hordeum vulgare; Pseudomonas syringae pv. tabaci; leaves; lipids; active transport; plant proteins; transgenic plants; necrosis; disease resistance; binding proteins
Abstract:
... Purified lipid transfer protein LTP2 from barley applied on tobacco leaves eliminated symptoms caused by infiltration of Pseudomonas syringae pv. tabaci 153. Growth of the pathogen in leaves of transgenic tobacco plants was retarded when compared with non-transformed controls. The percentage of inoculation points that showed necrotic lesions was greatly reduced in transgenic tobacco (17- 38% versu ...
DOI:
10.1046/j.1365-313X.1997.00669.x
PubMed:
9351251

66. Enhanced toxicity of sugar esters to the tobacco aphid (Homoptera: Aphididae) using humectants

Author:
Xia, Y.; Johnson, A.W.; Chortyk, O.T.
Source:
Journal of economic entomology 1997 v.90 no.4 pp. 1015-1021
ISSN:
0022-0493
Subject:
Nicotiana glutinosa; sucrose esters; botanical insecticides; Myzus persicae nicotianae; Nicotiana tabacum; sprayers; toxicity; insect control; spraying; mortality; South Carolina
Abstract:
... Eighteen humectants were evaluated in the laboratory and in the field to determine if they enhanced the insecticidal activity of natural and synthetic sugar esters against the tobacco aphid, Myzus nicotianae Blackman. Sugar esters included 3 types of mixtures of sucrose esters and glucose esters extracted from Nicotiana gossei Domin, N. palmeri Gray, and N. glutinosa L., and 4 types of synthetic s ...
DOI:
10.1093/jee/90.4.1015

67. Environmental signals modulate the expression of an indole-3-acetic acid biosynthetic gene in Erwinia herbicola

Author:
Brandl, M.T.; Lindow, S.E.
Source:
Molecular plant-microbe interactions 1997 v.10 no.4 pp. 499-505
ISSN:
0894-0282
Subject:
indolepyruvate decarboxylase; recombinant DNA; biosynthesis; structural genes; Pyrus communis; water potential; cell culture; osmotic pressure; ice nucleation; flowers; Phaseolus vulgaris; gene expression; Nicotiana tabacum; reporter genes; indole acetic acid; Pantoea agglomerans; culture media; leaves
Abstract:
... The ipdC gene of Erwinia herbicola strain 299R encodes an indolepyruvate decarboxylase involved in the biosynthesis of indole-3-acetic acid (IAA). Transcriptional fusions of ipdC to an ice nucleation reporter gene (inaZ) were used to study the expression of ipdC in vitro and in situ on plants. ipdC was expressed only at low levels in liquid media and independently of factors such as richness of th ...
DOI:
10.1094/MPMI.1997.10.4.499

68. Estimation of the anatomical, stomatal and biochemical components of differences in photosynthesis and transpiration of wild-type and transgenic (expressing yeast-derived invertase targeted to the vacuole) tobacco leaves

Author:
Pachepsky, L.B.; Acock, B.; Hoffman-Benning, S.; Willmitzer, L.; Fisahn, J.
Source:
Plant, cell and environment 1997 v.20 no.8 pp. 1070-1078
ISSN:
0140-7791
Subject:
mathematical models; phenotype; surface area; relative humidity; transgenic plants; yeasts; vacuoles; transpiration; light intensity; cells; leaf area; Nicotiana tabacum; beta-fructofuranosidase; diffusion; photosynthesis; stomata
Abstract:
... Photosynthesis and transpiration rates of transgenic (expressing yeast-derived invertase targeted to the vacuole) tobacco (Nicotiana tabacum L.) leaves were, respectively, 50 and 70% of those of a wild type at 20 degrees C, 350 cm3 m-3 CO2 concentration, 450 micromoles (photons) m-2 s-1 of light intensity, and 70% relative air humidity. These differences could be attributed: (a) to changes in leaf ...
DOI:
10.1111/j.1365-3040.1997.tb00683.x

69. Evaluation of Tobacco Accessions for Resistance to Tobaco Cyst Nematode and Wildlife

Author:
A. J. Hayes; C. A. Wilkinson; C. S. Johnson
Source:
Crop science 1997 v.37 no.2 pp. 586-591
ISSN:
0011-183X
Subject:
germplasm; cultivars; Globodera; genetic resistance; Nicotiana tabacum; disease resistance; screening; pest resistance; bacteria; Pseudomonas syringae pv. tabaci
Abstract:
... Control of tobacco cyst nematode (Globodera tabacum solanacearum Miller and Gray) is of major concern to growers of flue-cured tobacco (Nicotiana tabacum L.) in Virginia. A diverse geographic array of accessions including cultivars of several classes of tobacco, flue-cured type tobacco introductions, and Nicotiana species were evaluated during 1993 and 1994 in the greenhouse to identify new source ...
DOI:
10.2135/cropsci1997.0011183X003700020045x

70. Evaluation of five promoters for use in transformation of oil palm (Elaeis guineensis Jacq.)

Author:
Chowdhury, M.K.U.; Parveez, G.K.A.; Saleh, N.M.
Source:
Plant cell reports 1997 v.16 no.5 pp. 277-281
ISSN:
0721-7714
Subject:
Elaeis guineensis; Nicotiana tabacum; genetic transformation; gene transfer; beta-glucuronidase; enzyme activity; reporter genes; gene expression; callus; leaves; histochemistry; promoter regions
Abstract:
... The efficiency of GUS (beta-Glucuronidase) gene expression in embryogenic callus and young leaflets of mature and seedling palm after microprojectile bombardment with five constructs (pEmuGN, pAHC25, pAct1-F4, pGH24 and pBARGUS) was evaluated to identify the most suitable promoter(s) to use in transformation attempts in oil palm. Expression of the GUS gene driven by the Emu, Ubi1, Act1, 35S or Adh ...
DOI:
10.1007/s002990050222

71. Evolutionary aspects of "chloroplast-like" trnN and trnH expression in higher-plant mitochondria

Author:
Fey, J.; Dietrich, A.; Cosset, A.; Desprez, T.; Marechal-Drouard, L.
Source:
Current genetics 1997 v.32 no.5 pp. 358-360
ISSN:
0172-8083
Subject:
Solanum tuberosum; mitochondrial DNA; transfer RNA; asparagine; histidine; genes; nucleotide sequences; chloroplast DNA; chromosome mapping; Phaseolus vulgaris; Ipomoea batatas; Petunia; Nicotiana tabacum
DOI:
10.1007/s002940050288
PubMed:
9371887

72. Existence of three regulatory regions each containing a highly conserved motif in the promoter of plastid-encoded RNA polymerase gene (rpoB)

Author:
Inada, H.; Seki, M.; Morikawa, H.; Nishimura, M.; Iba, K.
Source:
plant journal 1997 v.11 no.4 pp. 883-890
ISSN:
0960-7412
Subject:
chloroplast DNA; promoter regions; DNA-directed RNA polymerase; assays; Nicotiana tabacum; cytochemistry; Arabidopsis thaliana; chloramphenicol; mutagenesis; transcription (genetics); histochemistry; genetic transformation; biolistics; transgenic plants; plasmid vectors; alpha-amanitin; plastids; cultured cells; reporter genes; genomics; beta-glucuronidase
Abstract:
... The plastid gene rpoB encodes a plastid-specific, DNA-dependent RNA polymerase. A study of a barley mutant, albostrians, indicated that rpoB itself is transcribed by a nuclear-encoded RNA polymerase. However, the molecular nature of the nuclear-encoded RNA polymerase and mechanisms of the transcriptional regulation of rpoB expression have not been elucidated. Using an in vivo transient assay syste ...
DOI:
10.1046/j.1365-313X.1997.11040883.x
PubMed:
9161043

73. Expression of a borage desaturase cDNA containing an N-terminal cytochrome b5 domain results in the accumulation of high levels of delta 6-desaturated fatty acids in transgenic tobacco

Author:
Sayanova, O.; Smith, M.A.; Lapinskas, P.; Stobart, A.K.; Dobson, G.; Christie, W.W.; Shewry, P.R.; Napier, J.A.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1997 v.94 no.8 pp. 4211-4216
ISSN:
0027-8424
Subject:
stearoyl-CoA desaturase; open reading frames; gene expression; Nicotiana tabacum; leaves; messenger RNA; seeds; complementary DNA; amino acid sequences; gamma-linolenic acid; genetic transformation; gene transfer; Borago officinalis; transgenic plants; cytochrome b; chemical constituents of plants
Abstract:
... gamma-Linolenic acid (GLA; C18:3 delta(6,9,12) is a component of the seed oils of evening primrose (Oenothera spp.), borage (Borago officinalis L.), and some other plants. It is widely used as a dietary supplement and for treatment of various medical conditions. GLA is synthesized by a delta(6)-fatty acid desaturase using linoleic acid (C18:2 delta(9,12) as a substrate. To enable the production of ...
DOI:
10.1073/pnas.94.8.4211
PubMed:
9108131
PubMed Central:
PMC20606

74. Expression of a luteoviral movement protein in transgenic plants leads to carbohydrate accumulation and reduced photosynthetic capacity in source leaves

Author:
Herbers, K.; Tacke, E.; Hazirezaei, M.; Krause, K.; Melzer, M.; Rohde, W.; Sonnewald, U.
Source:
plant journal 1997 v.12 no.5 pp. 1045-1056
ISSN:
0960-7412
Subject:
gas exchange; vacuoles; light intensity; height; pyrimidine nucleotides; carbon dioxide; glucose; starch; gene expression; Nicotiana tabacum; homeostasis; phosphates; Potato virus Y; fructose; photosynthesis; hexoses; leaves; sucrose; plant characteristics; adenosine triphosphate; translocation (plant physiology); phloem; pathogenesis-related proteins; ribulose-bisphosphate carboxylase; plasmodesmata; transgenic plants; Potato leafroll virus; viral proteins; chemical constituents of plants
Abstract:
... Elucidating the role of viral genes in transgenic plants revealed that the movement protein (MP) from tobacco mosaic virus is responsible for altered carbohydrate allocation in tobacco and potato plants. To study whether this is a general feature of viral MPs, the movement protein MP17 of potato leafroll virus (PLRV), a phloem-restricted luteovirus, was constitutively expressed in tobacco plants. ...
DOI:
10.1046/j.1365-313X.1997.12051045.x
PubMed:
9418046

75. Expression of an aspartate kinase homoserine dehydrogenase gene is subject to specific spatial and temporal regulation in vegetative tissues, flowers, and developing seeds

Author:
Zhu-Shimoni, J.X.; Lev-Yadun, S.; Matthews, B.; Galili, G.
Source:
Plant physiology 1997 v.113 no.3 pp. 695-706
ISSN:
0032-0889
Subject:
chemical reactions; developmental stages; genes; phosphotransferases (kinases); pollen; gene expression; Nicotiana tabacum; leaves; Arabidopsis thaliana; aspartic acid; enzyme activity; histochemistry; gene transfer; homoserine; biosynthesis; transgenic plants; flowers; biochemical pathways; oxidoreductases; plant morphology; seed development
Abstract:
... Although theregulation of amino acid synthesis has been studied extensively at the biochemical level, it is still not known how genes encoding amino acid biosynthesis enzymes are regulated during plant development. In the present report, we have used the beta-glucuronidase (GUS) reporter gene to study the regulation of expression of an Arabidopsis thaliana aspartate kinase-homoserine dehydrogenase ...

76. Expression of an engineered cecropin gene cassette in transgenic tobacco plants confers disease resistance to Pseudomonas syringae pv. tabaci

Author:
Huang, Y.; Nordeen, R.O.; Di, M.; Owens, L.D.; McBeath, J.H.
Source:
Phytopathology 1997 v.87 no.5 pp. 494-499
ISSN:
0031-949X
Subject:
Nicotiana tabacum; Pseudomonas syringae pv. tabaci; pathogenicity; disease resistance; plant breeding; gene transfer; transgenic plants; peptides; plasmids; genetic code; antibacterial properties
Abstract:
... A chimeric gene fusion cassette, consisting of a secretory sequence from barley alpha-amylase joined to a modified cecropin (MB39) coding sequence and placed under control of the promoter and terminator from the potato proteinase inhibitor II (PiII) gene, was introduced into tobacco by Agrobacterium-mediated transformation. Transgenic and control plants reacted differently when inoculated with tob ...
DOI:
10.1094/PHYTO.1997.87.5.494
PubMed:
18945103

77. Expression of heterologous phytochromes A, B or C in transgenic tobacco plants alters vegetative development and flowering time

Author:
Halliday, K.J.; Thomas, B.; Whitelam, G.C.
Source:
plant journal 1997 v.12 no.5 pp. 1079-1090
ISSN:
0960-7412
Subject:
height; genes; red light; far-red light; phenotype; hypocotyls; gene expression; Nicotiana tabacum; phytochrome; introgression; Arabidopsis thaliana; plant development; leaf area; photoperiod; dwarfing; transgenic plants; cotyledons; Avena sativa; photoperiodism
Abstract:
... In this study, oat phytochrome A (phyA), Arabidopsis phytochrome B (phyB) or Arabidopsis phytochrome C (phyC) were expressed in both day-neutral and photo-period-sensitive (short-day) tobacco (Nicotiana tabacum cv. Hicks), Introgression of the Maryland Mammoth (MM) gene into cv Hicks was used to confer short-day photo-periodic sensitivity. Expression of oat phyA led to characteristic hypersensitiv ...
DOI:
10.1046/j.1365-313X.1997.12051079.x
PubMed:
9418049

78. Expression of pokeweed antiviral protein in transgenic plants induced virus resistance in grafted wild-type plants independently of salicylic acid accumulation and pathogenesis-related protein synthesis

Author:
Smironv, S.; Shulaev, V.; Tumer, N.E.
Source:
Plant physiology 1997 v.114 no.3 pp. 1113-1121
ISSN:
0032-0889
Subject:
Nicotiana tabacum; Tobacco mosaic virus; Potato virus X; pathogenicity; disease resistance; plant breeding; gene transfer; transgenic plants; gene expression; genes; protein synthesis; Phytolacca americana; salicylic acid; quantitative analysis; pathogenesis-related proteins; chemical constituents of plants
Abstract:
... Pokeweed antiviral protein (PAP), a 29-kD protein isolated from Phytolacca americana, inhibits translation by catalytically removing a specific adenine residue from the large rRNA of the 60S subunit of eukaryotic ribosomes. Transgenic tobacco (Nicotiana tabacum) plants expressing PAP or a variant (PAP-v) were shown to be resistant to a broad spectrum of plant viruses. Expression of PAP-v in transg ...

79. Expression of the farnesyldiphosphate synthase gene of Saccharomyces cerevisiae in tobacco

Author:
S. Daudonnet; F. Karst; Y. Tourte
Source:
Molecular breeding 1997 v.3 no.2 pp. 137-145
ISSN:
1380-3743
Subject:
Cauliflower mosaic virus; Nicotiana tabacum; Saccharomyces cerevisiae; biosynthesis; carotenoids; cetyltrimethylammonium bromide; dithiothreitol; genes; heterologous gene expression; naphthaleneacetic acid; polyvinylpyrrolidone; promoter regions; tobacco; transgenic plants; yeasts
Abstract:
... The coding region of the farnesyldiphosphate synthase (FDP synthase) gene from Saccharomyces cerevisiae has been inserted into a pBin19 vector, downstream of the cauliflower mosaic virus (CaMV) 35S promoter, in order to allow its expression in the genome of a higher plant, Nicotiana tabacum. We have produced transgenic tobacco in which the expression of the foreign gene leads to functional FDP syn ...
DOI:
10.1023/A:1009685032495

80. Expression of the ribosome-inactivating protein JIP60 from barley in transgenic tobacco leads to an abnormal phenotype and alterations on the level of translation

Author:
Gorschen, E.; Dunaeva, M.; Hause, B.; Reeh, I.; Wasternack, C.; Parthier, B.
Source:
Planta 1997 v.202 no.4 pp. 470-478
ISSN:
0032-0935
Subject:
plant development; messenger RNA; protein synthesis; immunochemistry; phenotype; Hordeum vulgare; transgenic plants; jasmonic acid; meristems; internodes; vacuoles; ribosomes; gene expression; microsomes; protein synthesis inhibitors; Nicotiana tabacum; mesophyll; roots; leaves; plant proteins
Abstract:
... In this paper we report the in-planta activity of the ribosome-inactivating protein JIP60, a 60-kDa jasmonate-induced protein from barley (Hordeum vulgare L.), in transgenic tobacco (Nicotiana tabacum L.) plants. All plants expressing the complete JIP60 cDNA under the control of the cauliflower mosaic virus (CaMV) 35S promoter exhibited conspicuous and similar phenotypic alterations, such as slowe ...
DOI:
10.1007/s004250050151

81. Expression of the yeast trehalose-6-phosphate synthase gene in transgenic tobacco plants: pleiotropic phenotypes include drought tolerance

Author:
Romero, C.; Belles, J.M.; Vaya, J.L.; Serrano, R.; Culianez-Macia, F.A.
Source:
Planta 1997 v.201 no.3 pp. 293-297
ISSN:
0032-0935
Subject:
drought tolerance; genes; Agrobacterium; promoter regions; carbohydrate metabolism; phenotype; pleiotropy; glucose; water stress; gene expression; Nicotiana tabacum; phenotypic variation; shape; leaves; sucrose; ligases; water content; gene transfer; osmosis; transgenic plants; Saccharomyces cerevisiae; trehalose; Cauliflower mosaic virus; chemical constituents of plants
Abstract:
... The yeast trehalose-6-phosphate synthase gene (TPS1) was engineered under the control of the cauliflower mosaic virus regulatory sequences (CaMV35S) for expression in plants. Using Agrobacterium-mediated transfer, the gene was incorporated into the genomic DNA and constitutively expressed in Nicotiana tabacum L. plants. Trehalose was determined in the transformants, by anion-exchange chromatograph ...
DOI:
10.1007/s004250050069
PubMed:
19343407

82. Expression pattern of tobacco cyclin genes

Author:
Setiady, Y.Y.; Sekine, M.; Yamamoto, T.; Kouchi, H.; Shinmyo, A.
Source:
Plant cell reports 1997 v.16 no.6 pp. 368-372
ISSN:
0721-7714
Subject:
apical meristems; buds; complementary DNA; developmental stages; cell division; clones; gene expression; transcription (genetics); genes; Nicotiana tabacum; genetic variation; histochemistry; shoots
Abstract:
... We have previously isolated three cDNA clones (Ntcyc25, Ntcyc27, Ntcyc29) encoding mitotic cyclins from tobacco (Nicotiana tabacum) and in this report we describe the expression patterns of these genes. RNA gel-blot analysis showed that the mitotic cyclin genes were expressed predominantly in actively dividing meristematic tissues such as the shoot apex and young developing leaves. In situ hybridi ...
DOI:
10.1007/s002990050241

83. Finding of UDP-Glucose: Nicotinic Acid-N-glucosyltransferase Activity in Cultured Tobacco Cells and Its Properties

Author:
Hiroshi Taguchi; Kazuyoshi Sasatani; Hiroshi Nishitani; Katsuzumi Okumura
Source:
Bioscience, biotechnology, and biochemistry 1997 v.61 no.4 pp. 720-722
ISSN:
1347-6947
Subject:
Nicotiana tabacum; benzoic acid; biosynthesis; biotechnology; enzyme activity; heavy metals; metabolites; niacin; pH; temperature; tobacco
Abstract:
... We have already identified N-(β-d-glucopyranosyl)nicotinic acid, which was isolated from cultured tobacco cells, as a novel niacin metabolite. The enzyme activity for the biosynthesis of this compound, UDP-glucose: nicotinic acid-N-glucosyltransferase, was found in cell-free extracts from cultured cells of tobacco (Nicotiana tabacum XD-6) and properties were investigated in this experiment. The ge ...
DOI:
10.1271/bbb.61.720

84. Fourier-transform infrared and Raman spectroscopic evidence for the incorporation of cinnamaldehydes into the lignin of transgenic tobacco (Nicotiana tabacum L.) plants with reduced expression of cinnamyl alcohol dehydrogenase

Author:
Stewart, D.; Yahiaoui, N.; McDougall, G.J.; Myton, K.; Marque, C.; Boudet, A.M.; Haigh, J.
Source:
Planta 1997 v.201 no.3 pp. 311-318
ISSN:
0032-0935
Subject:
Nicotiana tabacum; transgenic plants; aldehydes; alcohol oxidoreductases; enzyme activity; antisense DNA; xylem; lignin; infrared spectroscopy; chemical structure; cell walls; biochemical pathways; biosynthesis; chemical constituents of plants; spectral analysis; nondestructive methods
Abstract:
... Xylem from stems of genetically manipulated tobacco plants which had had cinnamyl alcohol dehydrogenase (CAD; EC 1.1.1.195) activity down-regulated to a greater or lesser degree (clones 37 and 49, respectively) by the insertion of antisense CAD cDNA had similar, or slightly higher, lignin contents than xylem from wild-type plants. Fourier-transform infrared (FT-IR) microspectroscopy indicated that ...
DOI:
10.1007/s004250050072
PubMed:
19343409

85. Fructan of the inulin neoseries is synthesized in transgenic chicory plants (Cichorium intybus L.) harbouring onion (Allium cepa L.) fructan:fructan 6G-fructosyltransferase

Author:
Vijn, I.; Dijken, A. van.; Sprenger, N.; Dun, K. van.; Weisbeek, P.; Wiemken, A.; Smeekens, S.
Source:
plant journal 1997 v.11 no.3 pp. 387-398
ISSN:
0960-7412
Subject:
hexosyltransferases; carbohydrate metabolism; DNA libraries; kestose; complementary DNA; Allium cepa; transgenic plants; sucrose; plant extracts; amino acid sequences; beta-fructofuranosidase; nucleotide sequences; Nicotiana tabacum; inulin; Cichorium intybus
Abstract:
... Fructan (polyfructosylsucrose) is an important storage carbohydrate in many plant families. fructan:fructan 6G-fructosyltransferase (6G-FFT) is a key enzyme in the formation of the inulin neoseries, a type of fructan accumulated by members of the Liliales. We have cloned the 6G-FFT from onion by screening a cDNA library using barley sucrose fructan 6-fructosyltransferase (6-SFT) as a probe. The de ...
DOI:
10.1046/j.1365-313X.1997.11030387.x
PubMed:
9107030

86. Fundamental and biotechnological applications of phytochrome transgenes

Author:
Robson, P.R.H.; Smith, H.
Source:
Plant, cell and environment 1997 v.20 no.6 pp. 831-839
ISSN:
0140-7791
Subject:
genetic transformation; stem form; chemical constituents of plants; transgenic plants; seedlings; plant density; gene expression; polyploidy; genes; Nicotiana tabacum; harvest index; genetic engineering; maturity stage; transcription (genetics); phytochrome; photoreceptors; light; crops
Abstract:
... Individual phytochrome genes share regions of high homology which have facilitated the isolation of many phytochrome sequences from both higher and lower plants. Transgenes have been used to study the transcriptional control of phytochrome gene expression, and to create phytochrome-deficient lines in polyploid species. Transgenically produced phytochrome apoproteins assemble with endogenous chromo ...
DOI:
10.1046/j.1365-3040.1997.d01-106.x

87. Fungal and bacterial disease resistance in transgenic plants expressing human lysozyme

Author:
Nakajima, H.; Muranaka, T.; Ishige, F.; Akutsu, K.; Oeda, K.
Source:
Plant cell reports 1997 v.16 no.10 pp. 674-679
ISSN:
0721-7714
Subject:
lysozyme; genetic transformation; gene transfer; Erysiphe cichoracearum; conidia; humans; transgenic plants; Agrobacterium radiobacter; gene expression; Pseudomonas syringae; pathogenicity; Nicotiana tabacum; genes; disease resistance; plant pathogenic fungi
Abstract:
... The human lysozyme gene, which is assembled by the stepwise ligation of chemically synthesized oligo- nucleotides, was introduced into tobacco (Nicotiana tabacum cv 'SR1') by the Agrobacterium-mediated method. The introduced human lysozyme gene was highly expressed under the control of the cauliflower mosaic virus 35S promoter, and the gene product accumulated in the transgenic tobacco plants. The ...
DOI:
10.1007/s002990050300

88. A G-box-binding protein from soybean binds to the E1 auxin-response element in the soybean GH3 promoter and contains a proline-rich repression domain

Author:
Liu, Z.B.; Hagen, G.; Guilfoyle, T.J.
Source:
Plant physiology 1997 v.115 no.2 pp. 397-407
ISSN:
0032-0889
Subject:
protoplasts; gene transfer; Glycine max; leucine; genetic code; transgenic plants; Daucus carota; proline; luciferase; binding proteins; gene expression; plasmids; auxins; Nicotiana tabacum; transcription (genetics); reporter genes; enzyme activity; beta-glucuronidase
Abstract:
... The E1 promoter fragment (-249 to -203) is one of three auxin-response elements (AuxREs) in the soybean (Glycine max L.) GH3 promoter (Z.-B. Liu, T. Ulmasov, X. Shi, G. Hagen, T.J. Guilfoyle [1994] Plant Cell 6: 645-657). Results presented here further characterize and delimit the AuxRE within the E1 fragment. The E1 fragment functioned as an AuxRE in transgenic tobacco (Nicotiana tabacum L.) plan ...

89. Gating of epidermal plasmodesmata is restricted to the leading edge of expanding infection sites of tobacco mosaic virus (TMV)

Author:
Oparka, K.J.; Prior, D.A.M.; Santa Cruz, S.; Padgett, H.S.; Beachy, R.N.
Source:
plant journal 1997 v.12 no.4 pp. 781-789
ISSN:
0960-7412
Subject:
infection; Nicotiana tabacum; recombinant proteins; animal proteins; plasmodesmata; Tobacco mosaic virus; viral proteins; cytochemistry; histochemistry; dextran; pathogenesis; translocation (plant physiology)
Abstract:
... Plasmodesmatal gating in epidermal cells of Nicotiana tabacum was examined in expanding infection sites of tobacco mosaic virus (TMV) expressing a fusion between the viral movement protein and the green fluorescent protein (MP-GFP). The infection sites were circular in profile and within 3 days post-inoculation had developed a brightly fluorescent leading edge, giving them a characteristic 'halo' ...
DOI:
10.1046/j.1365-313X.1997.12040781.x
PubMed:
9375392

90. Geminivirus resistance in transgenic tobacco expressing mutated BC1 protein

Author:
Duan, Y.P.; Powell, C.A.; Webb, S.E.; Purcifull, D.E.; Hiebert, E.
Source:
Molecular plant-microbe interactions 1997 v.10 no.5 pp. 617-623
ISSN:
0894-0282
Subject:
Nicotiana tabacum; antisense DNA; Geminiviridae; transgenic plants; disease resistance; Bemisia tabaci; disease vectors; viral proteins; virus replication; gene expression; immunochemistry; leaves; plasmid vectors; genetic transformation; phenotype; explants
Abstract:
... Tobacco explants were transformed by Agrobacterium-mediated transformation with sense and antisense constructs of the movement protein genes (BC1 and BV1) of tomato mottle geminivirus (TMoV). Transgenic plants were tested for virus resistance either by exposure to viruliferous whiteflies carrying TMoV or cabbage leaf curl geminivirus (CabLCV) for a 72-h inoculation period or by continuous exposure ...
DOI:
10.1094/MPMI.1997.10.5.617

91. Gene fusions of signal sequences with a modified beta-glucuronidase gene results in retention of the beta-glucuronidase protein in the secretory pathway/plasma membrane

Author:
Yan, X.H.; Gonzales, R.A.; Wagner, G.J.
Source:
Plant physiology 1997 v.115 no.3 pp. 915-924
ISSN:
0032-0889
Subject:
Phaseolus vulgaris; Manduca sexta; Nicotiana tabacum; leaves; callus; endoplasmic reticulum; sucrose; biochemical pathways; protein composition; beta-glucuronidase; enzyme activity; reporter genes; transgenic plants; gene expression; cytosol; gene transfer; genetic vectors; genetic transformation; plasma membrane; translocation (plant physiology); plant secretions and exudates
Abstract:
... Signal sequences and endoplasmic reticulum (ER)retention signals are known to play central roles in targeting and translocation in the secretory pathway, but molecular aspects about their involvement are poorly understood. We tested the effectiveness of deduced signal sequences from various genes (hydroxyproline-rich glycoprotein [HRGP] from Phaseolus vulgaris; Serpin from Manduca sexta) to direct ...
PubMed:
9390428
PubMed Central:
PMC158555

92. Gene targeting and instability of Agrobacterium T-DNA loci in the plant genome

Author:
Risseeuw, E.; Franke-van Dijk, M.E.I.; Hooykaas, P.J.J.
Source:
plant journal 1997 v.11 no.4 pp. 717-728
ISSN:
0960-7412
Subject:
genome; flucytosine; micropropagation; Nicotiana plumbaginifolia; callus; drug resistance; DNA; Agrobacterium radiobacter; Nicotiana tabacum; leaves; plant development; enzyme activity; protoplasts; gene transfer; genetic transformation; cytosine deaminase; kanamycin; loci; transgenic plants; genetic stability; homologous recombination; nucleotide sequences; beta-glucuronidase
Abstract:
... To develop a model system for studies of homologous recombination in plants, transgenic Nicotiana tabacum and Nicotiana plumbaginifolia lines were generated harbouring a single target T-DNA containing the negative selective codA gene encoding cytosine deaminase (CD) and the beta-glucuronidase (GUS) gene. Subsequently, the target lines were transformed with a replacement-type T-DNA vector in which ...
DOI:
10.1046/j.1365-313X.1997.11040717.x
PubMed:
9161032

93. Green-fluorescent protein fusions for efficient characterization of nuclear targeting

Author:
Grebenok, R.J.; Pierson, E.; Lambert, G.M.; Gong, F.C.; Afonso, C.L.; Haldeman-Cahill, R.; Carrington, J.C.; Galbraith, D.W.
Source:
plant journal 1997 v.11 no.3 pp. 573-586
ISSN:
0960-7412
Subject:
Nicotiana tabacum; fluorescence; histochemistry; beta-glucuronidase; reporter genes; cytochemistry; gene expression; protein transport; oligopeptides; animal proteins; recombinant proteins; Scyphozoa; Aequorea victoria
Abstract:
... The green-fluorescent protein (GFP) from Aequorea victoria has been shown to be a convenient and flexible reporter molecule within a variety of eukaryotic systems, including higher plants. It is particularly suited for applications in vivo, since the mechanism of fluorophore formation involves an intramolecular autoxidation and does not require exogenous co-factors. Unlike standard histochemical p ...
DOI:
10.1046/j.1365-313X.1997.11030573.x
PubMed:
9107043

94. Heat stress affects the organization of microtubules and cell division in Nicotiana tabacum cells

Author:
Smertenko, A.; Draber, P.; Viklicky, V.; Opatrny, Z.
Source:
Plant, cell and environment 1997 v.20 no.12 pp. 1534-1542
ISSN:
0140-7791
Subject:
heat shock proteins; mitosis; microbodies; cycloheximide; heat stress; Nicotiana tabacum; microtubules; cell suspension culture; telophase
Abstract:
... To investigate the effects of heat stress on the plant cytoskeleton, the structure of microtubule arrays in N. tabacum suspension cells incubated at 38 or 42 degrees C was analysed. Whilst incubation at 42 degrees C resulted in the disruption of the majority of cellular microtubules after 30 min, in cells exposed to 38 degrees C all the microtubule arrays were preserved even after 12 h of incubati ...
DOI:
10.1046/j.1365-3040.1997.d01-44.x

95. Heat treatment results in a loss of transgene-encoded activities in several tobacco lines

Author:
Neumann, K.; Droge-Laser, W.; Kohne, S.; Broer, I.
Source:
Plant physiology 1997 v.115 no.3 pp. 939-947
ISSN:
0032-0889
Subject:
seedlings; luciferase; neomycin; heritability; heat treatment; phenotype; luminescence; Agrobacterium radiobacter; nopaline; heat stress; gene expression; Nicotiana tabacum; genetic code; ligases; phosphotransferases (phosphomutases); temperature; enzyme activity; line differences; genetic transformation; gene transfer; protein synthesis; transgenic plants; stress response; reporter genes; RNA; plant morphology
Abstract:
... Heat treatment (37 degrees C) of transgenic tobacco (Nicotiana tabacum) plants led to a reversible reduction or complete loss of transgene-encoded activities in about 40% of 10 independent transformants carrying the luciferase-coding region fused to the 355 cauliflower mosaic virus or the soybean small subunit promoter and the nopaline synthase promoter driving the neomycin phosphotransferase gene ...
PubMed:
9390430
PubMed Central:
PMC158557

96. High-affinity binding site for ethylene-inducing xylanase elicitor on Nicotiana tabacum membranes

Author:
Hanania, U.; Avni, A.
Source:
plant journal 1997 v.12 no.1 pp. 113-120
ISSN:
0960-7412
Subject:
protoplasts; glycosidases; cultivars; Solanum lycopersicum var. lycopersicum; Trichoderma viride; microsomes; hypersensitive response; Nicotiana tabacum; cell membranes; binding sites; cytochemistry; molecular weight; cell suspension culture; fluorescent dyes; defense mechanisms
Abstract:
... Challenge of Nicotiana tabacum cv Xanthi with the ethylene-inducing xylanase (EIX) from Trichoderma viride causes rapid induction of plant defense responses leading to hypersensitive necrosis. This phenomenon is cultivar-specific; no response is detected when N. tabacum cv Hicks is similarly treated. The responsiveness is determined in tobacco and tomato by a single dominant gene. EIX was labeled ...
DOI:
10.1046/j.1365-313X.1997.12010113.x

97. Hrp pilus: an hrp-dependent bacterial surface appendage produced by Pseudomonas syringae pv. tomato DC3000

Author:
Roine, E.; Wei, W.; Yuan, J.; Nurmiaho-Lassila, E.L.; Kalkkinen, N.; Romantschuk, M.; He, S.Y.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1997 v.94 no.7 pp. 3459-3464
ISSN:
0027-8424
Subject:
Arabidopsis thaliana; protein synthesis; structural genes; flagellum; Pseudomonas syringae pv. tomato; bacterial proteins; mutants; Nicotiana tabacum; pathogenesis; Solanum lycopersicum var. lycopersicum
Abstract:
... Hypersensitive response and pathogenicity (hrp) genes control the ability of major groups of plant pathogenic bacteria to elicit the hypersensitive response (HR) in resistant plants and to cause disease in susceptible plants. A number of Hrp proteins share significant similarities with components of the type III secretion apparatus and flagellar assembly apparatus in animal pathogenic bacteria. He ...
DOI:
10.1073/pnas.94.7.3459
PubMed:
9096416
PubMed Central:
PMC20392

98. Human haemoglobin from transgenic tobacco

Author:
Dieryck, W.; Pagnier, J.; Poyart, C.; Marden, M.C.; Gruber, V.; Bournat, P.; Baudino, S.; Merot, B.
Source:
Nature 1997 v.386 no.6620 pp. 29-30
ISSN:
0028-0836
Subject:
hemoglobin; biological production; plasmid vectors; transgenic plants; Nicotiana tabacum
DOI:
10.1038/386029b0
PubMed:
9052777

99. Hypoosmotic shock induces increases in cytosolic Ca2+ in tobacco suspension-culture cells

Author:
Takahashi, K.; Isobe, M.; Knight, M.R.; Trewavas, A.J.; Muto, S.
Source:
Plant physiology 1997 v.113 no.2 pp. 587-594
ISSN:
0032-0889
Subject:
Nicotiana tabacum; cell suspension culture; osmotic pressure; cytosol; calcium; luminescence; measurement; transgenic plants; gene expression; stress response
Abstract:
... Hypoosmotic shock treatment increased cytosolic Ca2+ ion concentration ([Ca2+]cyt) in tobacco (Nicotiana tabacum) suspension-culture cells. [Ca2+]cyt measurements were made by genetically transforming these cells to express apoaequorin and by reconstituting the Ca2+-dependent photoprotein, aequorin, in the cytosol by incubation with chemically synthesized coelenterazine. Measurement of Ca2+-depend ...

100. The IBP genes of maize are expressed in non-meristematic, elongating cells of the seedling and in abortive floral organs

Author:
Klinge, B.; Lange, T.; Werr, W.
Source:
MGG 1997 v.255 no.3 pp. 248-257
ISSN:
0026-8925
Subject:
transcription factors; inflorescences; Zea mays; messenger RNA; gene transfer; dwarfing; seedlings; transgenic plants; embryo (plant); transcription (genetics); gene expression; endosperm; Nicotiana tabacum; abnormal development; roots; shoots
DOI:
10.1007/s004380050495
PubMed:
9268015