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You searched for: Publication Year 1997 Remove constraint Publication Year: 1997 Subject Nicotiana tabacum Remove constraint Subject: Nicotiana tabacum
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1. A 146 bp fragment of the tobacco Lhcb1*2 promoter confers very-low-fluence, low-fluence and high-irradiance responses of phytochrome to a minimal CaMV 35S promoter

Author:
Pablo D. Cerdán; Roberto J. Staneloni; Jorge J. Casal; Rodolfo A. Sánchez
Source:
Plant molecular biology 1997 v.33 no.2 pp. 245-255
ISSN:
0167-4412
Subject:
Cauliflower mosaic virus; Nicotiana tabacum; beta-glucuronidase; far-red light; gene expression; genes; light intensity; phytochrome; promoter regions; red light; seedlings; tobacco; transgenic plants
Abstract:
... The occurrence of very-low-fluence responses (VLFR), low-fluence responses (LFR) and high-irradiance responses (HIR) of phytochrome was investigated for the expression of the gene of β-glucuronidase (gusA) under the control of the tobacco Lhcb1*2 promoter, in etiolated transgenic tobacco seedlings. The activity of β-glucuronidase (GUS) showed biphasic responses to the calculated proportion of Pf ...
DOI:
10.1023/A:1005784407282

2. AC Cheng flue-cured tobacco

Author:
Brandle, J.E.; Ankersmit, J.C.D.; Rogers, W.D.
Source:
Canadian journal of plant science 1997 v.77 no.1 pp. 155-156
ISSN:
0008-4220
Subject:
cultivars; hybrids; somatic hybridization; Nicotiana rustica; Nicotiana tabacum; Canada
DOI:
10.4141/P96-066

3. AC Gayed flue-cured tobacco

Author:
Brandle, J.E.; Rogers, W.D.; Ankersmit, J.C.D.
Source:
Canadian journal of plant science 1997 v.77 no.1 pp. 157-158
ISSN:
0008-4220
Subject:
Thielaviopsis basicola; cultivars; hybrids; Nicotiana debneyi; Nicotiana tabacum; disease resistance; plant pathogenic fungi; genes; Ontario
DOI:
10.4141/P96-067

4. AC Maridel flue-cured tobacco

Author:
Brandle, J.E.; Arsenault, W.; Rogers, W.D.; Ankersmit, J.C.D.
Source:
Canadian journal of plant science 1997 v.77 no.1 pp. 159-160
ISSN:
0008-4220
Subject:
Thielaviopsis basicola; Nicotiana tabacum; plant pathogenic fungi; disease resistance; high-yielding varieties; Prince Edward Island
DOI:
10.4141/P96-077

5. Accumulation of nitrate in the shoot acts as a signal to regulate shoot-root allocation in tobacco

Author:
Scheible, W.R.; Lauerer, M.; Schulze, E.D.; Caboche, M.; Stitt, M.
Source:
plant journal 1997 v.11 no.4 pp. 671-691
ISSN:
0960-7412
Subject:
nitrate reductase; nutrition-genotype interaction; roots; glutamate decarboxylase; shoots; carbohydrate metabolism; nitrogen content; sugars; starch; Nicotiana tabacum; genotype; phosphates; root shoot ratio; ammonium nitrate; leaves; glutamine; nutrient deficiencies; dry matter partitioning; genetic transformation; nutrient availability; mutants; chemical constituents of plants; protein content
Abstract:
... Mutants and transformants of tobacco (Nicotiana tabacum L. cv Gatersleben 1) with decreased expression of nitrate reductase have been used to investigate whether nitrate accumulation in the shoot acts as a signal to alter allocation between shoot and root growth. (a) Transformants with very low (1-3% of wild-type levels) nitrate reductase activity had growth rates, and protein, amino acid and glut ...
DOI:
10.1046/j.1365-313X.1997.11040671.x

6. Active oxygen species production in tobacco cells elicited by cryptogein

Author:
Simon-Plas, F.; Rusterucci, C.; Milat, M.L.; Humbert, C.; Montillet, J.L.; Blein, J.P.
Source:
Plant, cell and environment 1997 v.20 no.12 pp. 1573-1579
ISSN:
0140-7791
Subject:
proteins; extracellular fluids; free radicals; hydrogen peroxide; oxidoreductases; enzyme inhibitors; fusicoccin; pH; Nicotiana tabacum; alkalinization; plasma membrane; enzyme activity; oxygen; cell suspension culture; adenosinetriphosphatase; defense mechanisms
Abstract:
... We analyse the relationship between active oxygen species (AOS) production and pH changes induced in tobacco cells by cryptogein, a fungal proteinaceous elicitor of defence mechanisms in plants. When tobacco cells were treated with cryptogein, an intracellular acidification, an alkalinization of the extracellular medium and a transient burst of AOS (H2O2) were observed. Treatment of elicited cells ...
DOI:
10.1046/j.1365-3040.1997.d01-45.x

7. Aetiology of tobacco leaf curl in southern Africa

Author:
Paximadis, M.; Rey, M.E.C.
Source:
Annals of applied biology 1997 v.131 no.3 pp. 449-457
ISSN:
0003-4746
Subject:
Tobacco leaf curl Japan virus; etiology; leaves; Nicotiana tabacum; RNA; South Africa; Zimbabwe
DOI:
10.1111/j.1744-7348.1997.tb05172.x

8. Alfalfa mosaic virus movement protein induces tubules in plant protoplasts

Author:
Zheng, H.; Wang, G.; Zhang, L.
Source:
Molecular plant-microbe interactions 1997 v.10 no.8 pp. 1010-1014
ISSN:
0894-0282
Subject:
protoplasts; animal proteins; recombinant proteins; plasmid vectors; mutation; amino acid sequences; gene expression; Scyphozoa; viral proteins; Nicotiana tabacum; surfaces; transfection; Alfalfa mosaic virus
Abstract:
... The alfalfa mosaic virus (AIMV) movement protein (MP) was fused with the green fluorescence protein (GFP). The MP-GFP fusion was transiently expressed in protoplasts prepared from Nicotiana tabacum, resulting in the production of long, extending, tubular structures protruding from the protoplast surface. Deletions of MP amino acids 1 to 77, 84 to 142, or 226 to 300 all affected tubule formation. H ...
DOI:
10.1094/MPMI.1997.10.8.1010

9. Alternative processing of the maize Ac transcript in Arabidopsis

Author:
Martin, D.J.; Firek, S.; Moreau, E.; Draper, J.
Source:
plant journal 1997 v.11 no.5 pp. 933-945
ISSN:
0960-7412
Subject:
Arabidopsis thaliana; Zea mays; messenger RNA; alternative splicing; chemical reactions; transgenic plants; nucleotidyltransferases; Nicotiana tabacum; Nicotiana plumbaginifolia; introns; transposons
Abstract:
... The successful application of the maize transposable element system Ac/Ds as a genome mutagen in heterologous plant species has recently proved the versatility and power of this technique in plant molecular biology. However, the frequency of Ac/Ds transposition is considerably lower in Arabidopsis thaliana than in most other dicot plant species that have been studied. Since previous research has e ...
DOI:
10.1046/j.1365-313X.1997.11050933.x
PubMed:
9193067

10. Antigens produced in plants by infection with chimeric plant viruses immunize against rabies virus and HIV-1

Author:
Yusibov, V.; Modelska, A.; Steplewski, K.; Agadjanyan, M.; Weiner, D.; Hooper, D.C.; Koprowski, H.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1997 v.94 no.11 pp. 5784-5788
ISSN:
0027-8424
Subject:
coat proteins; recombinant antigens; Tobacco mosaic virus; Rabies lyssavirus; antibody formation; Alfalfa mosaic virus; recombinant proteins; plasmid vectors; gene expression; mice; Nicotiana tabacum; vaccination; vaccine development; viral antigens
Abstract:
... The coat protein (CP) of alfalfa mosaic virus was used as a carrier molecule to express antigenic peptides from rabies virus and HIV. The antigens were separately cloned into the reading frame of alfalfa mosaic virus CP and placed under the control of the subgenomic promoter of tobacco mosaic virus CP in the 30BRz vector. The in vitro transcripts of recombinant virus with sequences encoding the an ...
DOI:
10.1073/pnas.94.11.5784
PubMed:
9159151
PubMed Central:
PMC20857

11. Antimitotic effects of usnic acid on different biological systems

Author:
Cardarelli, M.; Serino, G.; Campanella, L.; Ercole, P.; De Cicco Nardone, F.; Alesiani, O.; Rossiello, F.
Source:
Cellular and molecular life sciences 1997 v.53 no.8 pp. 667-672
ISSN:
1420-682X
Subject:
protoplasts; seed germination; leukemia; humans; cytotoxicity; adenocarcinoma; lichenic acids; mesophyll; antifungal properties; Saccharomyces cerevisiae; cell division; Fusarium fujikuroi; Nicotiana tabacum; cell lines; metabolic inhibitors; lichens
PubMed:
9351470

12. Antisense expression and overexpression of biotin carboxylase in tobacco leaves

Author:
Shintani, D.; Roesler, K.; Shorrosh, B.; Savage, L.; Ohlrogge, J.
Source:
Plant physiology 1997 v.114 no.3 pp. 881-886
ISSN:
0032-0889
Subject:
1-aminocyclopropane-1-carboxylic acid; Nicotiana tabacum; leaves; plastids; fatty acids; biosynthesis; carboxy-lyases; biotin; enzyme activity; protein composition; quantitative analysis; genetic transformation; transgenic plants; antisense DNA; gene expression; genetic variation
Abstract:
... The plastid acetyl-coenzyme A carboxylase (ACCase) catalyzes the first committed step of fatty acid synthesis and in most plants is present as a heteromeric complex of at least four different protein subunits: the biotin carboxylase (BC), the biotin carboxyl carrier protein, and the alpha and beta subunits of the carboxyltransferase. To gain insight into the subunit organization of this heteromeri ...
PubMed:
9232874
PubMed Central:
PMC158375

13. The Arabidopsis MALE STERILITY 2 protein shares similarity with reductases in elongation/condensation complexes

Author:
Aarts, M.G.M.; Hodge, R.; Kalantidis, K.; Florack, D.; Wilson, Z.A.; Mulligan, B.J.; Stiekema, W.J.; Scott, R.; Pereira, A.
Source:
plant journal 1997 v.12 no.3 pp. 615-623
ISSN:
0960-7412
Subject:
fatty acids; flowering; pollen; promoter regions; plant proteins; cell walls; gene expression; Nicotiana tabacum; cell wall components; Arabidopsis thaliana; fatty alcohols; spores; amino acid sequences; histochemistry; transgenic plants; oxidoreductases; reporter genes; beta-glucuronidase
Abstract:
... The Arabidopsis thaliana MALE STERILITY 2 (MS2) gene product is involved in male gametogenesis. The first abnormalities in pollen development of ms2 mutants are seen at the stage in microsporogenesis when microspores are released from tetrads. Expression of the MS2 gene is observed in tapetum of wild-type flowers at, and shortly after, the release of microspores from tetrads. The MS2 promoter cont ...
DOI:
10.1046/j.1365-313X.1997.00615.x
PubMed:
9351246

14. Association of caffeoyl coenzyme A 3-O-methyltransferase expression with lignifying tissues in several dicot plants

Author:
Ye, Z.H.
Source:
Plant physiology 1997 v.115 no.4 pp. 1341-1350
ISSN:
0032-0889
Subject:
developmental stages; lignification; roots; stems; methylation; immunohistochemistry; gene expression; Nicotiana tabacum; protein composition; plant development; Glycine max; Zinnia violacea; phloem; xylem; lignin; Solanum lycopersicum var. lycopersicum; biosynthesis; caffeic acid; Medicago sativa; Forsythia suspensa; coenzyme A; transferases
Abstract:
... Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT) was previously shown to be associated with lignificationin both in vitro tracheary elements (TEs) and organs of zinnia (Zinnia elegans). However, it is not known whether this is a general pattern in dicot plants. To address this question, polyclonal antibodies against zinnia recombinant CCoAOMT fusion protein were raised and used for immunolocali ...
PubMed:
9414548
PubMed Central:
PMC158599

15. Auxin-cytokinin interactions in wild-type and transgenic tobacco

Author:
Eklof, S.; Astot, C.; Blackwell, J.; Moritz, T.; Olsson, O.; Sandberg, G.
Source:
Plant & cell physiology 1997 v.38 no.3 pp. 225-235
ISSN:
0032-0781
Subject:
biosynthesis; transgenic plants; internodes; metabolites; Nicotiana tabacum; cytokinins; indole acetic acid; genetic variation; leaves
Abstract:
... Cytokinins and auxins are important regulators of plant growth and development, but there is incomplete and conflicting evidence that auxins affect cytokinin metabolism and vice versa. We have investigated these interactions in Nicotiana tabacum L. by separate in planta manipulation of levels of the hormones followed by analysis of the induced changes in the metabolism of the other hormone. Cytoki ...
DOI:
10.1093/oxfordjournals.pcp.a029157

16. Auxin-induced epinasty of tobacco leaf tissues. A nonethylene-mediated response

Author:
Keller, C.P.; Van Volkenburgh, E.
Source:
Plant physiology 1997 v.113 no.2 pp. 603-610
ISSN:
0032-0889
Subject:
Nicotiana tabacum; leaves; cell growth; epinasty; dose response; acetic acid; ethylene; biosynthesis; naphthaleneacetic acid; aminooxyacetic acid; indole acetic acid
Abstract:
... Interveinal strips (10 x 1.5 mm) excised from growing tobacco (Nicotiana tabacum L. cv Xanthi) leaves curled >300 degrees when incubated for 20 h in 5 to 500 micromolar alpha-naphthalene acetic acid or 50 to 500 micromolar indole-3-acetic acid. Epinasty was not induced without auxin or by the auxin analog beta-naphthalene acetic acid, and less substantial epinasty was induced in midrib and vein se ...

17. Boron nutrition of cultured tobacco BY-2 cells. II. Characterization of the boron-polysaccharide complex

Author:
Kobayashi, M.; Ohno, K.; Matoh, T.
Source:
Plant & cell physiology 1997 v.38 no.6 pp. 676-683
ISSN:
0032-0781
Subject:
Nicotiana tabacum; cultured cells; cell growth; cell walls; cell membranes; boron; polysaccharides; quantitative analysis; plant nutrition; rhamnose; galacturonic acid; nutrient requirements; nutrient deficiencies; chemical constituents of plants; binding sites
Abstract:
... In cultured tobacco BY-2 cells, more than 90% of the cellular boron (B) occurs in the cell wall and a negligible amount of B is detected in the membrane fraction. Nearly 80% of the cell wall B binds to rhamnogalacturonan II(RG-II) to form a borate-dimeric RG-II complex. Monomeric RG-II is not detected in the cell wall, but it is detected in the extracellular polysaccharides. The complex is reconst ...
DOI:
10.1093/oxfordjournals.pcp.a029220

18. C-terminal deletion mutant of pokeweed antiviral protein inhibits viral infection but does not depurinate host ribosomes

Author:
Tumer, N.E.; Hwang, D.J.; Bonness, M.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1997 v.94 no.8 pp. 3866-3871
ISSN:
0027-8424
Subject:
genetic transformation; antiviral properties; complementary DNA; transgenic plants; Potato virus X; Saccharomyces cerevisiae; ribosomes; binding proteins; protein synthesis inhibitors; gene expression; Nicotiana tabacum; mutants; disease resistance; Phytolacca americana; N-glycoside hydrolases
Abstract:
... Pokeweed antiviral protein (PAP), a 29-kDa protein isolated from Phytolacca americana, inhibits translation by catalytically removing a specific adenine residue from the large rRNA of the 60S subunit of eukaryotic ribosomes. In addition to its ribosome-inactivating ability, PAP has potent antiviral activity against many plant and animal viruses, including HIV. We recently described the isolation a ...
DOI:
10.1073/pnas.94.8.3866
PubMed:
9108070
PubMed Central:
PMC20533

19. Calcium distribution in fertilized and unfertilized ovules and embryo sacs of Nicotiana tabacum L

Author:
Tian, H.Q.; Russell, S.D.
Source:
Planta 1997 v.202 no.1 pp. 93-105
ISSN:
0032-0935
Subject:
Nicotiana tabacum; ovules; pollination; flowering; plant organs; cytochemistry; cytoplasm; chemical precipitation; embryo (plant); pollen tubes; potassium; antimony; inorganic ions; calcium; translocation (plant physiology); cell wall components
Abstract:
... Potassium antimonate was used to localize Ca2+ in tobacco ovules from 0 to 7 d after anthesis in pollinated and emasculated flowers. Antimonate binds "loosely bound" Ca2+ into calcium antimonate; less-soluble forms are unavailable and free calcium usually escapes. Ovules are immature at anthesis. Abundant calcium precipitates in nucellar cells surrounding the micropylar canal. A difference between ...
DOI:
10.1007/s004250050107

20. Cell-cycle-regulated transcription of A- and B-type plant cyclin genes in synchronous cultures

Author:
Ito, M.; Marie-Claire, C.; Sakabe, M.; Ohno, T.; Hata, S.; Kouchi, H.; Hashimoto, J.; Fukuda, H.; Komamine, A.; Watanabe, A.
Source:
plant journal 1997 v.11 no.5 pp. 983-992
ISSN:
0960-7412
Subject:
messenger RNA; Catharanthus roseus; complementary DNA; cell division; amino acid sequences; gene expression; transcription (genetics); cultured cells; Nicotiana tabacum; reporter genes; promoter regions; plant proteins
Abstract:
... Synchronously dividing cell cultures of Catharanthus roseus were used to isolate cDNAs for two mitotic cyclins, named CYS and CYM. The deduced protein sequence of CYS is similar to that of A-type cyclins, and CYM belongs to the group of B-type cyclins. In a fashion similar to the pattern of expression seen for A-type and B-type cyclins in mammalian cells, CYS is expressed before CYM in C. roseus c ...
DOI:
10.1046/j.1365-313X.1997.11050983.x
PubMed:
9193070

21. Cell-to-cell movement of potato spindle tuber viroid

Author:
Ding, B.; Kwon, M.O.; Hammond, R.; Owens, R.
Source:
plant journal 1997 v.12 no.4 pp. 931-936
ISSN:
0960-7412
Subject:
guard cells; plasmodesmata; complementary DNA; Potato spindle tuber viroid; mesophyll; plasmids; Nicotiana tabacum; nucleotide sequences; symplast; RNA; fluorescent dyes; Solanum lycopersicum var. lycopersicum
Abstract:
... Viroids are non-translatable, autonomously replicating circular RNAs that infect only plants. An important component of the viroid infection process is cell-to-cell movement; however, there is virtually no information available about the pathways and mechanisms of this process. In this study, potato spindle tuber viroid (PSTVd) has been used as a model system to investigate the mechanism of viroid ...
DOI:
10.1046/j.1365-313X.1997.12040931.x
PubMed:
9375403

22. Cell-to-cell trafficking of cucumber mosaic virus movement protein:green fluorescent protein fusion produced by biolistic gene bombardment in tobacco

Author:
Itaya, A.; Hickman, H.; Bao, Y.; Nelsen, R.; Ding, B.
Source:
plant journal 1997 v.12 no.5 pp. 1223-1230
ISSN:
0960-7412
Subject:
Nicotiana tabacum; Cucumber mosaic virus; viral proteins; biolistics; transgenic plants; leaves; reporter genes; plasmodesmata; beta-glucuronidase; animal proteins; recombinant proteins; Scyphozoa; translocation (plant physiology)
Abstract:
... Previous micro-injection studies showed that some recombinant viral movement proteins and plant proteins produced in and purified from Escherichia coli could traffic from cell to cell. However, the relevance of these findings obtained by micro-injecting proteins produced in E. coli to the real functions of these proteins when produced in planta has been questioned. In this study, specific gene con ...
DOI:
10.1046/j.1365-313X.1997.12051223.x

23. Changes in the activity and mRNA of cinnamyl alcohol dehydrogenase during tracheary element differentiation in zinnia

Author:
Sato, Y.; Watanabe, T.; Komamine, A.; Hibino, T.; Shibata, D.; Sugiyama, M.; Fukuda, H.
Source:
Plant physiology 1997 v.113 no.2 pp. 425-430
ISSN:
0032-0889
Subject:
Zinnia violacea; mesophyll; lignin; biosynthesis; tracheids; cell differentiation; cinnamic acid; alcohol dehydrogenase; enzyme activity; messenger RNA; gene expression; complementary DNA; Eucalyptus; Nicotiana tabacum
Abstract:
... Changes in the enzymatic activity of cinnamyl alcohol dehydrogenase (CAD) and in the expression of a gene for CAD during tracheary element (TE) differentiation were investigated in cultures of single cells isolated from the mesophyll of zinnia (Zinnia elegans). In cultures in which TE differentiation was induced (TE-inductive cultures), CAD activity increased from h 36 after the start of culture ( ...
PubMed:
9046592
PubMed Central:
PMC158157

24. Characterization and functional expression of a ubiquitously expressed tomato pectin methylesterase

Author:
Gaffe, J.; Tiznado, M.E.; Handa, A.K.
Source:
Plant physiology 1997 v.114 no.4 pp. 1547-1556
ISSN:
0032-0889
Subject:
Solanum lycopersicum var. lycopersicum; Nicotiana tabacum; roots; leaves; protein composition; pectinesterase; enzyme activity; hypocotyls; epicotyls; cotyledons; developmental stages; protein synthesis; genes; gene expression; amino acid sequences; messenger RNA; transgenic plants; genetic code; cell wall components; transcription (genetics)
Abstract:
... Pectin methylesterase (PME), a ubiquitous enzyme in plants, de-esterifies the methoxylated pectin in the plant cell wall. We have characterized a PME gene (designated as pmeu1) from tomato (Lycopersicon esculentum) with an expression that is higher in younger root, leaf, and fruit tissues than in older tissues. Hypocotyls and epicotyls show higher accumulation of pmeu1 transcripts compared with co ...
PubMed:
9276962
PubMed Central:
PMC158449

25. Characterization of RNA-mediated resistance to tomato spotted wilt virus in transgenic tobacco plants expressing NSM gene sequences

Author:
Marcel Prins; Marjolein Kikkert; Cahya Ismayadi; William de Graauw; Peter de Haan; Rob Goldbach
Source:
Plant molecular biology 1997 v.33 no.2 pp. 235-243
ISSN:
0167-4412
Subject:
messenger RNA; antisense RNA; transgenic plants; gene expression; resistance mechanisms; Nicotiana tabacum; disease resistance; genes; nucleotide sequences; progeny; viral proteins; viruses; Tomato spotted wilt orthotospovirus
Abstract:
... Transgenic Nicotiana tabacum plants expressing RNA sequences of the tomato spotted wilt virus NSM gene, which encodes the putative viral movement protein, were found to be highly resistant to infection with the virus. Expression of untranslatable as well as anti-sense RNA of the NSM gene resulted in resistance levels as high as those in plants expressing translatable RNA sequences. For all three t ...
DOI:
10.1023/A:1005729808191

26. Characterization of a DNA-binding protein that interacts with 5' flanking regions of two fruit-ripening genes

Author:
Coupe, S.A.; Deikman, J.
Source:
plant journal 1997 v.11 no.6 pp. 1207-1218
ISSN:
0960-7412
Subject:
transcription factors; ethylene; messenger RNA; complementary DNA; plasmid vectors; amino acid sequences; gene expression; Nicotiana tabacum; ripening; DNA-binding proteins; genes; binding sites; promoter regions; Solanum lycopersicum var. lycopersicum
Abstract:
... The E4/E8 binding protein (E4/E8BP) interacts with sequences in the 5' flanking regions of two genes, E4 and E8, that are coordinately regulated by ethylene during tomato fruit ripening. The DNA-binding activity of this protein increases during fruit ripening, and it may play a role in regulation of these genes. To begin to understand the function of this protein, a cDNA has been isolated that enc ...
DOI:
10.1046/j.1365-313X.1997.11061207.x
PubMed:
9225464

27. Characterization of a diffusible signal capable of inducing defense gene expression in tobacco

Author:
Chappell, J.; Levine, A.; Tenhaken, R.; Lusso, M.; Lamb, C.
Source:
Plant physiology 1997 v.113 no.2 pp. 621-629
ISSN:
0032-0889
Subject:
Nicotiana tabacum; cell suspension culture; Phytophthora cryptogea; plant pathogenic fungi; pathogenicity; disease resistance; genes; phytoalexins; biosynthesis; lyases; enzyme activity; measurement; gene expression; Glycine max; hydrogen peroxide; pathogenesis-related proteins; sesquiterpenoids
Abstract:
... Treatment of tobacco (Nicotiana tabacum) cell-suspension cultures with cryptogein, an elicitin protein from Phytophthora cryptogea, resulted in the release of a factor(s) that diffused through a 1000-D cutoff dialysis membrane and was capable of inducing sesquiterpene cyclase enzyme activity (a key phytoalexin biosynthetic enzyme in solanaceous plants) when added to fresh cell-suspension cultures. ...

28. Characterization of a tobacco extensin gene and regulation of its gene family in healthy plants and under various stress conditions

Author:
Cathy Hirsinger; Yves Parmentier; Andrée Durr; Jacqueline Fleck; Elisabeth Jamet
Source:
Plant molecular biology 1997 v.33 no.2 pp. 279-289
ISSN:
0167-4412
Subject:
DNA; Nicotiana sylvestris; Nicotiana tabacum; Nicotiana tomentosiformis; cell suspension culture; clones; crosslinking; extensin; gene expression regulation; genomic libraries; genomics; leaves; messenger RNA; molecular cloning; multigene family; plant damage; plant ovary; roots; seed germination; seeds; stems; tobacco
Abstract:
... A genomic clone (Ext 1.4) encoding an extensin was isolated from a Nicotiana tabacum genomic library. The encoded polypeptide showed features characteristic of extensins such as Ser-(Pro)4 repeats and a high content in Tyr and Lys residues. The presence of one Tyr-Leu-Tyr-Lys motif suggests the possibility for one intramolecular isodityrosine cross-link whereas numerous Val-Tyr-Lys motifs may part ...
DOI:
10.1023/A:1005738815383

29. Characterization of antisense transformed plants deficient in the tobacco anionic peroxidase

Author:
Lagrimini, L.M.; Gingas, V.; Finger, F.; Rothstein, S.; Liu, T.T.Y.
Source:
Plant physiology 1997 v.114 no.4 pp. 1187-1196
ISSN:
0032-0889
Subject:
cell growth; chemical constituents of plants; Nicotiana sylvestris; developmental stages; lignification; genes; roots; antisense RNA; phenotype; stems; cell walls; Nicotiana tabacum; genotype; peroxidase; leaves; defense mechanisms; genetic code; morphogenesis; transcription (genetics); xylem; lignin; enzyme activity; isozymes; transgenic plants; stress response; genetic variation; plant morphology
Abstract:
... On the basis of the biological compoundsthat they metabolize, plant peroxidases have long been implicated in plant growth, cell wall biogenesis, lignification, and host defenses. Transgenic tobacco (Nicotiana tabacum L.) plants that underexpress anionic peroxidase were generated using antisense RNA. The antisense RNA was found to be specific for the anionic isoenzyme and highly effective, reducing ...

30. Characterization of regions within the n-terminal 6-kilodalton domain of phytochrome A that modulate its biological activity

Author:
Jordan, E.T.; Marita, J.M.; Clough, R.C.; Vierstra, R.D.
Source:
Plant physiology 1997 v.115 no.2 pp. 693-704
ISSN:
0032-0889
Subject:
Avena sativa; seedlings; photoreceptors; red light; far-red light; phytochrome; apoproteins; plant development; alanine; serine; amino acid sequences; gene transfer; transgenic plants; Nicotiana tabacum; gene expression; genetic variation; light harvesting complex
Abstract:
... Phytochrome A (phyA) is a red/far-red (FR) light photoreceptor responsible for initiating numerous light-mediated plant growth and developmental responses, especially in FR light-enriched environments. We previously showed that the first 70 amino acids of the polypeptide contain at least two regions with potentially opposite functions (E.T. Jordan, J.R. Cherry, J.M. Walker, R.D. Vierstra [1996] Pl ...
PubMed:
9342873
PubMed Central:
PMC158530

31. Characterization of the targeted nuclear accumulation of GFP within the cells of transgenic plants

Author:
Grebenok, R.J.; Lambert, G.M.; Galbraith, D.W.
Source:
plant journal 1997 v.12 no.3 pp. 685-696
ISSN:
0960-7412
Subject:
Nicotiana tabacum; transgenic plants; cytoplasm; active transport; molecular weight; amino acid sequences; plant proteins; cytochemistry; spatial distribution; leaves; beta-glucuronidase; enzyme activity; animal proteins; Scyphozoa; green fluorescent protein; Aequorea victoria
Abstract:
... The soluble proteins of the nucleoplasm are synthesized on cytoplasmic ribosomes. Proteins larger than about 40 kDa are post-translationally targeted to the nucleus via energy-dependent processes, passing through the nuclear pore complex into the nucleoplasm. Targeting involves nuclear localization signals (NLSs) found within the primary sequences of the imported proteins. In higher plants, inform ...
DOI:
10.1046/j.1365-313X.1997.00685.x

32. Cloning and characterization of a cDNA encoding an elicitor of Phytophthora parasitica var. nicotianae that shows cellulose-binding and lectin-like activities

Author:
Mateos, F.V.; Richauer, M.; Esquerre-Tugaye, M.T.
Source:
Molecular plant-microbe interactions 1997 v.10 no.9 pp. 1045-1053
ISSN:
0894-0282
Subject:
Phytophthora nicotianae var. parasitica; complementary DNA; cellulose; lectins; binding proteins; glycoproteins; amino acid sequences; nucleotide sequences; cysteine; hydrolysis; hemagglutination; Nicotiana tabacum; leaves; necrosis; gene expression; defense mechanisms; virulence; disease resistance; cell wall components
Abstract:
... Phytophthora parasitica var. nicotianae produces a 34-kDa glycoprotein elicitor (CBEL) that is localized in the cell wall. A cDNA encoding (he protein moiety of this elicitor was cloned and characterized. The deduced amino acid sequence consisted of two direct repeats of a cysteine-rich domain, joined by a Thr/pro-rich region. Although having no general homology with published sequences, the posit ...
DOI:
10.1094/MPMI.1997.10.9.1045
PubMed:
9390419

33. Composition of photosystem II antenna in light-harvesting complex II antisense tobacco plants at varying irradiances

Author:
Flachman, R.
Source:
Plant physiology 1997 v.113 no.3 pp. 787-794
ISSN:
0032-0889
Subject:
Nicotiana tabacum; thylakoids; photosystem I; photosystem II; light intensity; genes; genetic code; quantitative analysis; chemical constituents of plants; light harvesting complex; transcription (genetics); phenotype
Abstract:
... Plants with genes coding for chlorophyll a/b-binding proteins of light-harvesting complex II (LHCII) in antisense orientation (Lhcb) that are characterized by severely reduced Lhcb transcript levels (below 10% of wild type) do not show a bleached phenotype due to a specific loss of the polypeptide. To produce such a phenotype, a conceptually different antisense approach was tested with a dual-func ...
PubMed:
9085572
PubMed Central:
PMC158197

34. Compromising early salicylic acid accumulation delays the hypersensitive response and increases viral dispersal during lesion establishment in TMV-infected tobacco

Author:
Mur, L.A.J.; Bi, Y.M.; Darby, R.M.; Firek, S.; Draper, J.
Source:
plant journal 1997 v.12 no.5 pp. 1113-1126
ISSN:
0960-7412
Subject:
Nicotiana tabacum; Tobacco mosaic virus; salicylic acid; defense mechanisms; gene expression; oxygenases; transgenic plants; necrosis; virulence; messenger RNA; beta-glucuronidase; reporter genes; pathogenesis; translocation (plant physiology); promoter regions
Abstract:
... To investigate the role of salicylic acid (SA) in the hypersensitive response (HR) its accumulation was compromised during different phases of lesion development by differential expression of a salicylate hydroxylase gene (SH-L). Constitutive suppression of SA accumulation was achieved by expression of a gene fusion between the CaMV35S promoter (35S) and SH-L. Using the H2O2-responsive AoPR1 promo ...
DOI:
10.1046/j.1365-313X.1997.12051113.x
PubMed:
9418052

35. Conditional transgenic expression of the ipt gene indicates a function for cytokinins in paracrine signaling in whole tobacco plants

Author:
Faiss, M.; Zalubilova, J.; Strnad, M.; Schmulling, T.
Source:
plant journal 1997 v.12 no.2 pp. 401-415
ISSN:
0960-7412
Subject:
buds; transgenic plants; meristems; zeatin; dihydrozeatin; transpiration; biochemical pathways; gene expression; glucosides; cytokinins; genes; Nicotiana tabacum; alkyl (aryl) transferases; roots; promoter regions; leaves; shoots
Abstract:
... This study investigated whether an increased production of the plant hormone cytokinin in roots, the main site of its synthesis and putative signaling organ, can influence developmental events, such as growth of axillary shoot meristems or leaf senescence, in the plant shoot. To this end, transgenic tobacco plants (Nicotiana tabacum L.) were generated that conditionally overproduce cytokinins. The ...
DOI:
10.1046/j.1365-313X.1997.12020401.x
PubMed:
9301091

36. Development of a binary vector system for plant transformation based on the supervirulent Agrobacterium tumefaciens strain Chry5

Author:
Torisky, R.S.; Kovacs, L.; Avdiushko, S.; Newman, J.D.; Hunt, A.G.; Collins, G.B.
Source:
Plant cell reports 1997 v.17 no.2 pp. 102-108
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; Glycine max; transgenic plants; Agrobacterium radiobacter; strain differences; virulence; plasmids; chromosome mapping; Nicotiana tabacum; genetic vectors; genetic markers
Abstract:
... This report describes the disarming of Agrobacterium tumefaciens Chry5, a strain highly tumorigenic on soybean. Disarming was achieved by removing an approximately 16.5-kb segment of the 285-kb Ti plasmid pTiChry5, including approximately 4 kb of the oncogenic T-DNA and an extended region right of the T-DNA, and replacing it with a gene for carbenicillin resistance, through homologous recombinatio ...
DOI:
10.1007/s002990050360

37. Development of necrosis and activation of disease resistance in transgenic tobacco plants with severely reduced catalase levels

Author:
Takahashi, H.; Chen, Z.; Du, H.; Liu, Y.; Klessig, D.F.
Source:
plant journal 1997 v.11 no.5 pp. 993-1005
ISSN:
0960-7412
Subject:
Nicotiana tabacum; transgenic plants; catalase; disease resistance; necrosis; enzyme activity; salicylic acid; binding proteins; antisense RNA; protein synthesis; chlorosis; Tobacco mosaic virus; nucleotide sequences; amino acid sequences; gene expression; pathogenesis-related proteins; chemical constituents of plants
Abstract:
... Numerous studies argue that salicylic acid (SA) is an important component of the plant signal transduction pathway(s) leading to disease resistance. The discovery that the SA-binding protein is a catalase, whose activity is blocked by SA, led to the proposal that one of SA's modes of action is to inhibit this H2O2-degrading enzyme and thus elevate H2O2 levels. To test this model, an attempt was ma ...
DOI:
10.1046/j.1365-313X.1997.11050993.x
PubMed:
9193071

38. Developmental changes in DNA methylation of the two tobacco pollen nuclei during maturation

Author:
Oakeley, E.J.; Podesta, A.; Jost, J.P.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1997 v.94 no.21 pp. 11721-11725
ISSN:
0027-8424
Subject:
Nicotiana tabacum; pollen; DNA methylation; gametogenesis; cytosine; monoclonal antibodies
Abstract:
... Changes in DNA methylation during tobacco pollen development have been studied by confocal fluorescence microscopy using a monoclonal anti-5-methylcytosine (anti-m5C) antibody and a polyclonal anti-histone H1 (anti-histone) antibody as an internal standard. The specificity of the anti-m5C antibody was demonstrated by a titration series against both single-stranded DNA and double-stranded DNA subst ...
DOI:
10.1073/pnas.94.21.11721
PubMed:
9326677
PubMed Central:
PMC23612

39. Differential accumulation of salicylic acid and salicylic acid-sensitive catalase in different rice tissues

Author:
Chen, Z.; Iyer, S.; Caplan, A.; Klessig, D.F.; Fan, B.
Source:
Plant physiology 1997 v.114 no.1 pp. 193-201
ISSN:
0032-0889
Subject:
Oryza sativa; Nicotiana tabacum; shoots; roots; cell suspension culture; leaves; salicylic acid; biosynthesis; quantitative analysis; catalase; enzyme activity; genes; gene expression; genetic code; chemical constituents of plants
Abstract:
... We previously proposed that salicylic acid (SA)-sensitive catalases serve as biological targets of SA in plant defense responses. To further examine the role of SA-sensitive catalases, we have analyzed the relationship between SA levels and SA sensitivity of catalases in different rice (Oryza sativa) tissues. We show here that, whereas rice shoots contain extremely high levels of free SA, as previ ...

40. Differential activation of two ACC oxidase gene promoters from melon during plant development and in response to pathogen attack

Author:
Lasserre, E.; Godard, F.; Bouquin, T.; Hernandez, J.A.; Pech, J.C.; Roby, D.; Balague, C.
Source:
MGG 1997 v.256 no.3 pp. 211-222
ISSN:
0026-8925
Subject:
ethylene; recombinant DNA; Ralstonia solanacearum; 2,4-D; promoter regions; gene expression; Nicotiana tabacum; mechanical damage; Pseudomonadaceae; oxygenases; infection; genetic recombination; copper sulfate; defense mechanisms; plant development; Cucumis melo; abiotic stress; histochemistry; transgenic plants; reporter genes; abscisic acid; beta-glucuronidase
DOI:
10.1007/s004380050563
PubMed:
9393445

41. Differential gene expression in response to auxin treatment in the wild type and rac, an adventitious rooting-incompetent mutant of tobacco

Author:
Lund, S.T.; Smith, A.G.; Hackett, W.P.
Source:
Plant physiology 1997 v.114 no.4 pp. 1197-1206
ISSN:
0032-0889
Subject:
cortex; messenger RNA; cell growth; transgenic plants; developmental stages; cell division; adventitious roots; gene expression; phloem; rooting; genes; Nicotiana tabacum; mutants; auxins; genotype; plant cuttings; histochemistry; callus
Abstract:
... Histological analyses of auxin-treated cuttings from the wildtype and the rac mutant of tobacco (Nicotiana tabacum cv Xanthii) previously revealed that some rac phloem parenchyma or inner cortical parenchyma cells form callus in response to exogenous auxin treatment but these cells never undergo the organized divisions associated with adventitious root initiation in the wild type. Here we report t ...

42. Directed overexpression of PHYA locally suppresses stem elongation and leaf senescence responses to far-red radiation

Author:
Rousseaux, M.C.; Ballare, C.L.; Jordan, E.T.; Vierstra, R.D.
Source:
Plant, cell and environment 1997 v.20 no.12 pp. 1551-1558
ISSN:
0140-7791
Subject:
nitrate reductase; genes; promoter regions; shoots; far-red light; gene expression; Nicotiana tabacum; phytochrome; leaves; Arabidopsis thaliana; weight; complementary DNA; morphogenesis; ubiquitin; enzyme activity; canopy; transgenic plants; internodes; plant morphology; chemical constituents of plants; chlorophyll
Abstract:
... Ectopic overexpression of an oat PHYA cDNA in tobacco under the cauliflower mosaic virus 35S promoter results in plants with reduced morphological responses to far-red radiation (FR). We have tested the hypothesis that it is possible to molecularly 'mask' stems and leaves to FR-induced elongation and senescence responses by targeting the overexpression of PHYA with appropriate promoters. Oat PHYA ...
DOI:
10.1046/j.1365-3040.1997.d01-51.x

43. Distribution profiles of dry matter and total nitrogen in leaves and stems of oriental field-grown tobacco plants at different nitrogen levels

Author:
Drossopoulos, J.B.; Bouranis, D.L.; Aivalakis, G.; Tsomokos, A.
Source:
Journal of plant nutrition 1997 v.20 no.6 pp. 695-713
ISSN:
0190-4167
Subject:
weight; nitrogen content; stems; nutrient availability; Nicotiana tabacum; dry matter partitioning; application rate; ammonium nitrate; leaves
Abstract:
... Oriental tobacco plants (Nicotiana tabacum L. cv Myrodata Agrinion) were grown without nitrogen (N) fertilization (N0) and with added ammonium nitrate at a rate of 50 kg. ha-1 (N1) and 100 kg.ha-1 (N2). Non-uniform patterns for leaf FW and DW changes per node showed a decreasing trend from lower to upper nodes during the vegetative stage. From approaching flowering to fruit set, these patterns bec ...
DOI:
10.1080/01904169709365287

44. Do plant phenolics confer resistance to specialist and generalist insect herbivores?

Author:
Bi, J.L.; Felton, G.W.; Murphy, J.B.; Howles, P.A.; Dixon, R.A.; Lamb, C.J.
Source:
Journal of agricultural and food chemistry 1997 v.45 no.11 pp. 4500-4504
ISSN:
0021-8561
Subject:
Manduca sexta; Heliothis virescens; pest resistance; phenolic compounds; phenylalanine ammonia-lyase; Nicotiana tabacum; chemical constituents of plants
Abstract:
... The impact of phenolics on a specialist herbivore, Manduca sexta, and a generalist herbivore, Heliothis virescens, was investigated using transgenic tobacco with differential expression of phenylalanine ammonia-lyase. Foliar phenolics such as chlorogenic acid, rutin, and total flavonoids differentially accumulated in the respective transgenic tobacco lines; the amount of chlorogenic acid ranged fr ...
DOI:
10.1021/jf970555m

45. Dual effect of phytochrome A on hypocotyl growth under continuous red light

Author:
Mazzella, M.A.; Magliano, T.M.A.; Casal, J.J.
Source:
Plant, cell and environment 1997 v.20 no.2 pp. 261-267
ISSN:
0140-7791
Subject:
Arabidopsis thaliana; Avena sativa; Nicotiana tabacum; transgenic plants; phytochrome; red light; hypocotyls; length; seedling growth; photoperiod; light intensity; temporal variation; mutants; far-red light
Abstract:
... The role of phytochrome A in the control of hypocotyl growth under continuous red light (Rc) was investigated using phyA and phyB mutants of Arabidopsis thaliana, which lack phytochrome A (phyA) or phytochrome B (phyB), respectively, and transgenic seedlings of Nicotiana tabacum overexpressing Avena phyA, compared to the corresponding wild type (WT). In WT seedlings of A. thaliana, hypocotyl growt ...
DOI:
10.1046/j.1365-3040.1997.d01-62.x

46. Ectopic expression of a tobacco homeobox gene, NTH15, dramatically alters leaf morphology and hormone levels in transgenic tobacco

Author:
Tamaoki, M.; Kusaba, S.; Kano-Murakami, Y.; Matsuoka, M.
Source:
Plant & cell physiology 1997 v.38 no.8 pp. 917-927
ISSN:
0032-0781
Subject:
chemical constituents of plants; developmental stages; genes; abnormal development; shoots; phenotype; gibberellic acid; gene expression; Nicotiana tabacum; leaves; apical meristems; quantitative analysis; complementary DNA; morphogenesis; amino acid sequences; indole acetic acid; gene transfer; transgenic plants; zeatin; flowers; nucleotide sequences; abscisic acid; plant morphology
Abstract:
... The shoot apical meristem functions to generate the lateral organs of a plant throughout the vegetative and reproductive phases. Homeobox genes play key roles in controlling such developmental programs, but their modes of action have not been well defined. Here we describe isolation and biological functions of a novel tobacco homeobox gene, designated NTH15 (Nicotiana tabacum homeobox 15), from a ...
DOI:
10.1093/oxfordjournals.pcp.a029252
PubMed:
9327591

47. Effectiveness of the bacterial gene codA encoding cytosine deaminase as a negative selectable marker in Agrobacterium-mediated plant transformation

Author:
Schlaman, H.R.M.; Hooykaas, P.J.J.
Source:
plant journal 1997 v.11 no.6 pp. 1377-1385
ISSN:
0960-7412
Subject:
amidine hydrolases; genetic transformation; protoplasts; plant development; callus; kanamycin; transgenic plants; Agrobacterium radiobacter; seedlings; plasmid vectors; explants; flucytosine; Nicotiana tabacum; leaves; culture media; roots; genetic markers; shoots
Abstract:
... The usefulness of the E. coli codA gene encoding cytosine deaminase as a conditional toxic gene was explored during various stages of plant development and in different Agrobacterium-mediated transformation protocols. To this end, several independent tobacco lines transgenic for codA were isolated and these were tested for their sensitivity to 5-fluorocytosine (5-FC) at different developmental sta ...
DOI:
10.1046/j.1365-313X.1997.11061377.x

48. Effects of leaf surface moisture and relative humidity on the efficacy of sugar esters from Nicotiana gossei against the tobacco aphid (Homoptera: Aphididae)

Author:
Xia, Y.; Johnson, A.W.
Source:
Journal of economic entomology 1997 v.90 no.4 pp. 1010-1014
ISSN:
0022-0493
Subject:
relative humidity; sucrose esters; botanical insecticides; Myzus persicae nicotianae; water content; Nicotiana tabacum; toxicity; insect control; spraying; mortality; leaves; South Carolina
Abstract:
... The effects of leaf surface moisture and relative humidity on the toxicity of sugar esters extracted from Nicotiana gossei Domin to the tobacco aphid, Myzus nicotianae Blackman, were examined in the laboratory and the field. The efficacy of sugar esters applied at a concentration of 1 mg/ml against tobacco aphids was >5 times higher under moist conditions (either wet leaf surface or wet filter pap ...
DOI:
10.1093/jee/90.4.1010

49. Efficient in vitro transcription of plant nuclear tRNASer genes in a nuclear extract from tobacco cultured cells

Author:
Yukawa, Y.; Sugita, M.; Sugiura, M.
Source:
plant journal 1997 v.12 no.4 pp. 965-970
ISSN:
0960-7412
Subject:
Nicotiana tabacum; cell suspension culture; genes; plant extracts; transfer RNA; DNA-directed RNA polymerase; transcription (genetics)
Abstract:
... A nuclear extract derived from tobacco cultured BY-2 cells supports RNA polymerase III-dependent transcription of Arabidopsis tRNA(Ser) genes. Primer extension analysis indicated that the transcription starts at 6 bp upstream from the 5' end of tRNA coding region. Procedures for nuclear extraction and in vitro reaction conditions have been optimized for tRNA transcription, which allows direct dete ...
DOI:
10.1046/j.1365-313X.1997.12040965.x
PubMed:
9375407

50. Elevated CO2 effects during leaf ontogeny: a new perspective on acclimation

Author:
Miller, A.; Tsai, C.H.; Hemphill, D.; Endres, M.; Rodermel, S.; Spalding, M.
Source:
Plant physiology 1997 v.115 no.3 pp. 1195-1200
ISSN:
0032-0889
Subject:
ribulose-bisphosphate carboxylase; carbon dioxide; developmental stages; net assimilation rate; acclimation; ontogeny; Nicotiana tabacum; photosynthesis; enzyme activity; leaves
Abstract:
... For many plants growth in elevated CO2 leads to reduced rates of photosynthesis. To examine the role that leaf ontogeny plays in the acclimation response, we monitored photosynthesis and some related parameters at short intervals throughout the ontogenetic development of tobacco (Nicotiana tabacum L.) leaves under ambient (350 microliters L-1)- and high (950 microliters L-1)-CO2 conditions. The pa ...

51. Encapsidation of potyviral RNA in various forms of transgene coat protein is not correlated with resistance in transgenic plants

Author:
Hammond, J.; Dienelt, M.M.
Source:
Molecular plant-microbe interactions 1997 v.10 no.8 pp. 1023-1027
ISSN:
0894-0282
Subject:
plant viruses; Nicotiana glutinosa; coat proteins; phenotype; transgenic plants; Nicotiana benthamiana; recombinant proteins; Nicotiana tabacum; disease resistance; Bean yellow mosaic virus; genes; RNA; virus replication
Abstract:
... Transgenic plants expressing either bean yellow mosaic potyvirus or chimeric potyvirus coat protein (CP) were inoculated with various potyviruses. Antigen-coated plate, indirect enzyme-linked immunosorbent assay and immunnoelectron microscopy of virus purified from transgenic plants showed that progeny virions contained from <1% to as much as 25% transgenic CP. Different levels of transcapsidation ...
DOI:
10.1094/MPMI.1997.10.8.1023
PubMed:
9353947

52. Enhanced tolerance to bacterial pathogens caused by the transgenic expression of barley lipid transfer protein LTP2

Author:
Molina, A.; Garcia-Olmedo, F.
Source:
plant journal 1997 v.12 no.3 pp. 669-675
ISSN:
0960-7412
Subject:
Nicotiana tabacum; Arabidopsis thaliana; Hordeum vulgare; Pseudomonas syringae pv. tabaci; leaves; lipids; active transport; plant proteins; transgenic plants; necrosis; disease resistance; binding proteins
Abstract:
... Purified lipid transfer protein LTP2 from barley applied on tobacco leaves eliminated symptoms caused by infiltration of Pseudomonas syringae pv. tabaci 153. Growth of the pathogen in leaves of transgenic tobacco plants was retarded when compared with non-transformed controls. The percentage of inoculation points that showed necrotic lesions was greatly reduced in transgenic tobacco (17- 38% versu ...
DOI:
10.1046/j.1365-313X.1997.00669.x
PubMed:
9351251

53. Estimation of the anatomical, stomatal and biochemical components of differences in photosynthesis and transpiration of wild-type and transgenic (expressing yeast-derived invertase targeted to the vacuole) tobacco leaves

Author:
Pachepsky, L.B.; Acock, B.; Hoffman-Benning, S.; Willmitzer, L.; Fisahn, J.
Source:
Plant, cell and environment 1997 v.20 no.8 pp. 1070-1078
ISSN:
0140-7791
Subject:
mathematical models; phenotype; surface area; relative humidity; transgenic plants; yeasts; vacuoles; transpiration; light intensity; cells; leaf area; Nicotiana tabacum; beta-fructofuranosidase; diffusion; photosynthesis; stomata
Abstract:
... Photosynthesis and transpiration rates of transgenic (expressing yeast-derived invertase targeted to the vacuole) tobacco (Nicotiana tabacum L.) leaves were, respectively, 50 and 70% of those of a wild type at 20 degrees C, 350 cm3 m-3 CO2 concentration, 450 micromoles (photons) m-2 s-1 of light intensity, and 70% relative air humidity. These differences could be attributed: (a) to changes in leaf ...
DOI:
10.1111/j.1365-3040.1997.tb00683.x

54. Evaluation of Tobacco Accessions for Resistance to Tobaco Cyst Nematode and Wildlife

Author:
A. J. Hayes; C. A. Wilkinson; C. S. Johnson
Source:
Crop science 1997 v.37 no.2 pp. 586-591
ISSN:
0011-183X
Subject:
germplasm; cultivars; Globodera; genetic resistance; Nicotiana tabacum; disease resistance; screening; pest resistance; bacteria; Pseudomonas syringae pv. tabaci
Abstract:
... Control of tobacco cyst nematode (Globodera tabacum solanacearum Miller and Gray) is of major concern to growers of flue-cured tobacco (Nicotiana tabacum L.) in Virginia. A diverse geographic array of accessions including cultivars of several classes of tobacco, flue-cured type tobacco introductions, and Nicotiana species were evaluated during 1993 and 1994 in the greenhouse to identify new source ...
DOI:
10.2135/cropsci1997.0011183X003700020045x

55. Evolutionary aspects of "chloroplast-like" trnN and trnH expression in higher-plant mitochondria

Author:
Fey, J.; Dietrich, A.; Cosset, A.; Desprez, T.; Marechal-Drouard, L.
Source:
Current genetics 1997 v.32 no.5 pp. 358-360
ISSN:
0172-8083
Subject:
Solanum tuberosum; mitochondrial DNA; transfer RNA; asparagine; histidine; genes; nucleotide sequences; chloroplast DNA; chromosome mapping; Phaseolus vulgaris; Ipomoea batatas; Petunia; Nicotiana tabacum
DOI:
10.1007/s002940050288
PubMed:
9371887

56. Expression of a borage desaturase cDNA containing an N-terminal cytochrome b5 domain results in the accumulation of high levels of delta 6-desaturated fatty acids in transgenic tobacco

Author:
Sayanova, O.; Smith, M.A.; Lapinskas, P.; Stobart, A.K.; Dobson, G.; Christie, W.W.; Shewry, P.R.; Napier, J.A.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1997 v.94 no.8 pp. 4211-4216
ISSN:
0027-8424
Subject:
stearoyl-CoA desaturase; open reading frames; gene expression; Nicotiana tabacum; leaves; messenger RNA; seeds; complementary DNA; amino acid sequences; gamma-linolenic acid; genetic transformation; gene transfer; Borago officinalis; transgenic plants; cytochrome b; chemical constituents of plants
Abstract:
... gamma-Linolenic acid (GLA; C18:3 delta(6,9,12) is a component of the seed oils of evening primrose (Oenothera spp.), borage (Borago officinalis L.), and some other plants. It is widely used as a dietary supplement and for treatment of various medical conditions. GLA is synthesized by a delta(6)-fatty acid desaturase using linoleic acid (C18:2 delta(9,12) as a substrate. To enable the production of ...
DOI:
10.1073/pnas.94.8.4211
PubMed:
9108131
PubMed Central:
PMC20606

57. Expression of a luteoviral movement protein in transgenic plants leads to carbohydrate accumulation and reduced photosynthetic capacity in source leaves

Author:
Herbers, K.; Tacke, E.; Hazirezaei, M.; Krause, K.; Melzer, M.; Rohde, W.; Sonnewald, U.
Source:
plant journal 1997 v.12 no.5 pp. 1045-1056
ISSN:
0960-7412
Subject:
gas exchange; vacuoles; light intensity; height; pyrimidine nucleotides; carbon dioxide; glucose; starch; gene expression; Nicotiana tabacum; homeostasis; phosphates; Potato virus Y; fructose; photosynthesis; hexoses; leaves; sucrose; plant characteristics; adenosine triphosphate; translocation (plant physiology); phloem; pathogenesis-related proteins; ribulose-bisphosphate carboxylase; plasmodesmata; transgenic plants; Potato leafroll virus; viral proteins; chemical constituents of plants
Abstract:
... Elucidating the role of viral genes in transgenic plants revealed that the movement protein (MP) from tobacco mosaic virus is responsible for altered carbohydrate allocation in tobacco and potato plants. To study whether this is a general feature of viral MPs, the movement protein MP17 of potato leafroll virus (PLRV), a phloem-restricted luteovirus, was constitutively expressed in tobacco plants. ...
DOI:
10.1046/j.1365-313X.1997.12051045.x
PubMed:
9418046

58. Expression of an aspartate kinase homoserine dehydrogenase gene is subject to specific spatial and temporal regulation in vegetative tissues, flowers, and developing seeds

Author:
Zhu-Shimoni, J.X.; Lev-Yadun, S.; Matthews, B.; Galili, G.
Source:
Plant physiology 1997 v.113 no.3 pp. 695-706
ISSN:
0032-0889
Subject:
chemical reactions; developmental stages; genes; phosphotransferases (kinases); pollen; gene expression; Nicotiana tabacum; leaves; Arabidopsis thaliana; aspartic acid; enzyme activity; histochemistry; gene transfer; homoserine; biosynthesis; transgenic plants; flowers; biochemical pathways; oxidoreductases; plant morphology; seed development
Abstract:
... Although theregulation of amino acid synthesis has been studied extensively at the biochemical level, it is still not known how genes encoding amino acid biosynthesis enzymes are regulated during plant development. In the present report, we have used the beta-glucuronidase (GUS) reporter gene to study the regulation of expression of an Arabidopsis thaliana aspartate kinase-homoserine dehydrogenase ...

59. Expression of an engineered cecropin gene cassette in transgenic tobacco plants confers disease resistance to Pseudomonas syringae pv. tabaci

Author:
Huang, Y.; Nordeen, R.O.; Di, M.; Owens, L.D.; McBeath, J.H.
Source:
Phytopathology 1997 v.87 no.5 pp. 494-499
ISSN:
0031-949X
Subject:
Nicotiana tabacum; Pseudomonas syringae pv. tabaci; pathogenicity; disease resistance; plant breeding; gene transfer; transgenic plants; peptides; plasmids; genetic code; antibacterial properties
Abstract:
... A chimeric gene fusion cassette, consisting of a secretory sequence from barley alpha-amylase joined to a modified cecropin (MB39) coding sequence and placed under control of the promoter and terminator from the potato proteinase inhibitor II (PiII) gene, was introduced into tobacco by Agrobacterium-mediated transformation. Transgenic and control plants reacted differently when inoculated with tob ...
DOI:
10.1094/PHYTO.1997.87.5.494
PubMed:
18945103

60. Expression of heterologous phytochromes A, B or C in transgenic tobacco plants alters vegetative development and flowering time

Author:
Halliday, K.J.; Thomas, B.; Whitelam, G.C.
Source:
plant journal 1997 v.12 no.5 pp. 1079-1090
ISSN:
0960-7412
Subject:
height; genes; red light; far-red light; phenotype; hypocotyls; gene expression; Nicotiana tabacum; phytochrome; introgression; Arabidopsis thaliana; plant development; leaf area; photoperiod; dwarfing; transgenic plants; cotyledons; Avena sativa; photoperiodism
Abstract:
... In this study, oat phytochrome A (phyA), Arabidopsis phytochrome B (phyB) or Arabidopsis phytochrome C (phyC) were expressed in both day-neutral and photo-period-sensitive (short-day) tobacco (Nicotiana tabacum cv. Hicks), Introgression of the Maryland Mammoth (MM) gene into cv Hicks was used to confer short-day photo-periodic sensitivity. Expression of oat phyA led to characteristic hypersensitiv ...
DOI:
10.1046/j.1365-313X.1997.12051079.x
PubMed:
9418049

61. Expression of pokeweed antiviral protein in transgenic plants induced virus resistance in grafted wild-type plants independently of salicylic acid accumulation and pathogenesis-related protein synthesis

Author:
Smironv, S.; Shulaev, V.; Tumer, N.E.
Source:
Plant physiology 1997 v.114 no.3 pp. 1113-1121
ISSN:
0032-0889
Subject:
Nicotiana tabacum; Tobacco mosaic virus; Potato virus X; pathogenicity; disease resistance; plant breeding; gene transfer; transgenic plants; gene expression; genes; protein synthesis; Phytolacca americana; salicylic acid; quantitative analysis; pathogenesis-related proteins; chemical constituents of plants
Abstract:
... Pokeweed antiviral protein (PAP), a 29-kD protein isolated from Phytolacca americana, inhibits translation by catalytically removing a specific adenine residue from the large rRNA of the 60S subunit of eukaryotic ribosomes. Transgenic tobacco (Nicotiana tabacum) plants expressing PAP or a variant (PAP-v) were shown to be resistant to a broad spectrum of plant viruses. Expression of PAP-v in transg ...

62. Expression of the farnesyldiphosphate synthase gene of Saccharomyces cerevisiae in tobacco

Author:
S. Daudonnet; F. Karst; Y. Tourte
Source:
Molecular breeding 1997 v.3 no.2 pp. 137-145
ISSN:
1380-3743
Subject:
Cauliflower mosaic virus; Nicotiana tabacum; Saccharomyces cerevisiae; biosynthesis; carotenoids; cetyltrimethylammonium bromide; dithiothreitol; genes; heterologous gene expression; naphthaleneacetic acid; polyvinylpyrrolidone; promoter regions; tobacco; transgenic plants; yeasts
Abstract:
... The coding region of the farnesyldiphosphate synthase (FDP synthase) gene from Saccharomyces cerevisiae has been inserted into a pBin19 vector, downstream of the cauliflower mosaic virus (CaMV) 35S promoter, in order to allow its expression in the genome of a higher plant, Nicotiana tabacum. We have produced transgenic tobacco in which the expression of the foreign gene leads to functional FDP syn ...
DOI:
10.1023/A:1009685032495

63. Fructan of the inulin neoseries is synthesized in transgenic chicory plants (Cichorium intybus L.) harbouring onion (Allium cepa L.) fructan:fructan 6G-fructosyltransferase

Author:
Vijn, I.; Dijken, A. van.; Sprenger, N.; Dun, K. van.; Weisbeek, P.; Wiemken, A.; Smeekens, S.
Source:
plant journal 1997 v.11 no.3 pp. 387-398
ISSN:
0960-7412
Subject:
hexosyltransferases; carbohydrate metabolism; DNA libraries; kestose; complementary DNA; Allium cepa; transgenic plants; sucrose; plant extracts; amino acid sequences; beta-fructofuranosidase; nucleotide sequences; Nicotiana tabacum; inulin; Cichorium intybus
Abstract:
... Fructan (polyfructosylsucrose) is an important storage carbohydrate in many plant families. fructan:fructan 6G-fructosyltransferase (6G-FFT) is a key enzyme in the formation of the inulin neoseries, a type of fructan accumulated by members of the Liliales. We have cloned the 6G-FFT from onion by screening a cDNA library using barley sucrose fructan 6-fructosyltransferase (6-SFT) as a probe. The de ...
DOI:
10.1046/j.1365-313X.1997.11030387.x
PubMed:
9107030

64. A G-box-binding protein from soybean binds to the E1 auxin-response element in the soybean GH3 promoter and contains a proline-rich repression domain

Author:
Liu, Z.B.; Hagen, G.; Guilfoyle, T.J.
Source:
Plant physiology 1997 v.115 no.2 pp. 397-407
ISSN:
0032-0889
Subject:
protoplasts; gene transfer; Glycine max; leucine; genetic code; transgenic plants; Daucus carota; proline; luciferase; binding proteins; gene expression; plasmids; auxins; Nicotiana tabacum; transcription (genetics); reporter genes; enzyme activity; beta-glucuronidase
Abstract:
... The E1 promoter fragment (-249 to -203) is one of three auxin-response elements (AuxREs) in the soybean (Glycine max L.) GH3 promoter (Z.-B. Liu, T. Ulmasov, X. Shi, G. Hagen, T.J. Guilfoyle [1994] Plant Cell 6: 645-657). Results presented here further characterize and delimit the AuxRE within the E1 fragment. The E1 fragment functioned as an AuxRE in transgenic tobacco (Nicotiana tabacum L.) plan ...

65. Gene fusions of signal sequences with a modified beta-glucuronidase gene results in retention of the beta-glucuronidase protein in the secretory pathway/plasma membrane

Author:
Yan, X.H.; Gonzales, R.A.; Wagner, G.J.
Source:
Plant physiology 1997 v.115 no.3 pp. 915-924
ISSN:
0032-0889
Subject:
Phaseolus vulgaris; Manduca sexta; Nicotiana tabacum; leaves; callus; endoplasmic reticulum; sucrose; biochemical pathways; protein composition; beta-glucuronidase; enzyme activity; reporter genes; transgenic plants; gene expression; cytosol; gene transfer; genetic vectors; genetic transformation; plasma membrane; translocation (plant physiology); plant secretions and exudates
Abstract:
... Signal sequences and endoplasmic reticulum (ER)retention signals are known to play central roles in targeting and translocation in the secretory pathway, but molecular aspects about their involvement are poorly understood. We tested the effectiveness of deduced signal sequences from various genes (hydroxyproline-rich glycoprotein [HRGP] from Phaseolus vulgaris; Serpin from Manduca sexta) to direct ...
PubMed:
9390428
PubMed Central:
PMC158555

66. Gene targeting and instability of Agrobacterium T-DNA loci in the plant genome

Author:
Risseeuw, E.; Franke-van Dijk, M.E.I.; Hooykaas, P.J.J.
Source:
plant journal 1997 v.11 no.4 pp. 717-728
ISSN:
0960-7412
Subject:
genome; flucytosine; micropropagation; Nicotiana plumbaginifolia; callus; drug resistance; DNA; Agrobacterium radiobacter; Nicotiana tabacum; leaves; plant development; enzyme activity; protoplasts; gene transfer; genetic transformation; cytosine deaminase; kanamycin; loci; transgenic plants; genetic stability; homologous recombination; nucleotide sequences; beta-glucuronidase
Abstract:
... To develop a model system for studies of homologous recombination in plants, transgenic Nicotiana tabacum and Nicotiana plumbaginifolia lines were generated harbouring a single target T-DNA containing the negative selective codA gene encoding cytosine deaminase (CD) and the beta-glucuronidase (GUS) gene. Subsequently, the target lines were transformed with a replacement-type T-DNA vector in which ...
DOI:
10.1046/j.1365-313X.1997.11040717.x
PubMed:
9161032

67. Hypoosmotic shock induces increases in cytosolic Ca2+ in tobacco suspension-culture cells

Author:
Takahashi, K.; Isobe, M.; Knight, M.R.; Trewavas, A.J.; Muto, S.
Source:
Plant physiology 1997 v.113 no.2 pp. 587-594
ISSN:
0032-0889
Subject:
Nicotiana tabacum; cell suspension culture; osmotic pressure; cytosol; calcium; luminescence; measurement; transgenic plants; gene expression; stress response
Abstract:
... Hypoosmotic shock treatment increased cytosolic Ca2+ ion concentration ([Ca2+]cyt) in tobacco (Nicotiana tabacum) suspension-culture cells. [Ca2+]cyt measurements were made by genetically transforming these cells to express apoaequorin and by reconstituting the Ca2+-dependent photoprotein, aequorin, in the cytosol by incubation with chemically synthesized coelenterazine. Measurement of Ca2+-depend ...

68. Identification of cDNAs encoding sterol methyl-transferases involved in the second methylation step of plant sterol biosynthesis

Author:
Bouvier-Nave, P.; Husselstein, T.; Desprez, T.; Benveniste, P.
Source:
European journal of biochemistry 1997 v.246 no.2 pp. 518-529
ISSN:
0014-2956
Subject:
Arabidopsis thaliana; Glycine max; Nicotiana tabacum; Ricinus communis; S-adenosylmethionine; amino acid sequences; biosynthesis; catalytic activity; clones; complementary DNA; growth and development; methylation; methyltransferases; microsomes; mutants; nuclear magnetic resonance spectroscopy; phytosterols; plant growth; plant identification; yeasts
Abstract:
... Two methyl transfers are involved in the course of plant sterol biosynthesis and responsible for the formation of 24-alkyl sterols (mainly 24-ethyl sterols) which play major roles in plant growth and development. The first methyl transfer applies to cycloartenol, the second one to 24-methylene lophenol. Five cDNA clones encoding two Arabidopsis thaliana, two Nicotiana tabacum and one Ricinus commu ...
DOI:
10.1111/j.1432-1033.1997.t01-1-00518.x
PubMed:
9208946

69. Identification of calreticulin-like protein as one of the phosphoproteins modulated in response to oligogalacturonides in tobacco cells

Author:
Droillard, M.J.; Guclu, J.; Le Caer, J.P.; Mathieu, Y.; Guern, J.; Lauriere, C.
Source:
Planta 1997 v.202 no.3 pp. 341-348
ISSN:
0032-0935
Subject:
phosphoprotein phosphatase; phosphoproteins; amino acid sequences; Nicotiana tabacum; plasma membrane; phosphorylation; molecular weight; oligosaccharides; galacturonic acid; plant proteins
Abstract:
... Oligogalacturonide-induced modifications of protein phosphorylation in cells of Nicotiana tabacum L. were investigated by in-vitro phosphorylation of plasma-membrane-enriched fractions and electrophoretic analysis on two-dimensional gels. About 100 polypeptides were resolved; among these 40 phosphoproteins were detected and their 33P-labelling quantified. Most of the phosphorylations were inhibite ...
DOI:
10.1007/s004250050136
PubMed:
9232905

70. Identification of the peroxisomal targeting signal for cottonseed catalase

Author:
Mullen, R.T.; Lee, M.S.; Trelease, R.N.
Source:
plant journal 1997 v.12 no.2 pp. 313-322
ISSN:
0960-7412
Subject:
genetic transformation; peroxisomes; biolistics; catalase; Gossypium hirsutum; transgenic plants; cytosol; peptides; Nicotiana tabacum; signal peptide; chloramphenicol acetyltransferase; active transport; nucleotide sequences; immunocytochemistry; glyoxysomes; cell suspension culture
Abstract:
... Catalase is a ubiquitous peroxisomal matrix enzyme, yet the molecular targeting signal(s) for sorting it in plant cells has not been defined. The most common peroxisome targeting signal (PTS) is a C-terminal tripeptide composed of a conserved SKL motif (type 1 PTS). The PTS for cottonseed catalase (Ccat) was elucidated in this study from immunofluorescence microscopic analyses of tobacco BY-2 susp ...
DOI:
10.1046/j.1365-313X.1997.12020313.x
PubMed:
9301084

71. Importin alpha from Arabidopsis thaliana is a nuclear import receptor that recognizes three classes of import signals

Author:
Smith, H.M.S.; Hicks, G.R.; Raikhel, N.V.
Source:
Plant physiology 1997 v.114 no.2 pp. 411-417
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; Nicotiana tabacum; protein composition; protein transport; receptors; binding proteins; protoplasts; plant anatomy; plasma membrane
Abstract:
... Protein import into the nucleus is a two-step process. In vitro import systems from vertebrate cell extracts have shown that several soluble factors are required. One of these factors is the receptor importin alpha, which binds to nuclear localization signals (NLS) in vitro. We previously cloned an importin alpha homolog from Arabidopsis thaliana (At-IMP alpha) and demonstrated that this protein w ...
PubMed:
9193081
PubMed Central:
PMC158320

72. Increased resistance to oxidative stress in transgenic plants by targeting mannitol biosynthesis to chloroplasts

Author:
Shen, B.; Jensen, R.G.; Bohnert, H.J.
Source:
Plant physiology 1997 v.113 no.4 pp. 1177-1183
ISSN:
0032-0889
Subject:
Nicotiana tabacum; oxygen; leaves; chloroplasts; mannitol; biosynthesis; chlorophyll; quantitative analysis; photosynthesis; net assimilation rate; gene transfer; bacteria; genes; oxidoreductases; enzyme activity; wild relatives; transgenic plants; gene expression; chemical constituents of plants; genotype; reactive oxygen species; stress response
Abstract:
... To investigate the potential role of a polyol, mannitol, in oxidative stress protection, a bacterial mannitol-1-phosphate dehydrogenase gene was targeted to chloroplasts by the addition of an amino-terminal transit peptide. Transgenic tobacco (Nicotiana tabacum) lines accumulate mannitol at concentrations ranging from 2.5 to 7 micromoles/g fresh weight. Line BS1-31 accumulated approximately 100 mM ...
PubMed:
9112772
PubMed Central:
PMC158240

73. Increased salt and drought tolerance by D-ononitol production in transgenic Nicotiana tabacum L

Author:
Sheveleva, E.; Chmara, W.; Bohnert, H.J.; Jensen, R.G.
Source:
Plant physiology 1997 v.115 no.3 pp. 1211-1219
ISSN:
0032-0889
Subject:
chemical constituents of plants; drought tolerance; drought; measurement; metabolites; glucose; proline; Nicotiana tabacum; fructose; photosynthesis; quantitative analysis; complementary DNA; sucrose; myo-inositol; net assimilation rate; enzyme activity; salt tolerance; salinity; transgenic plants; stress response; diurnal variation; genetic variation; stomata; transferases; leaf conductance
Abstract:
... A cDNA encoding myo-inositol O-methyltransferase (IMT1) has been transferred into Nicotiana tabacum cultivar SR1. During drought and salt stress, transformants (I5A) accumulated the methylated inositol D-ononitol in amounts exceeding 35 micromoles g-1 fresh weight In I5A, photosynthetic CO2 fixation was inhibited less during salt stress and drought, and the plants recovered faster than wild type. ...

74. Inheritance of resistance to race 0 of Phytophthora parasitica var. nicotianae from the flue-cured tobacco cultivar Coker 371-Gold

Author:
Carlson, S.R.; Wolff, M.F.; Shew, H.D.; Wernsman, E.A.
Source:
Plant disease 1997 v.81 no.11 pp. 1269-1274
ISSN:
0191-2917
Subject:
Nicotiana tabacum; Phytophthora nicotianae var. parasitica; races; disease resistance; genetic resistance; screening; germplasm; fungal diseases of plants; inheritance (genetics); artificial selection; North Carolina
Abstract:
... Black shank, caused by Phytophthora parasitica var. nicotianae, is a widespread and severe disease of tobacco throughout the southeastern United States. Partial resistance derived from the cigar tobacco cultivar Florida 301 has been the primary means of reducing losses to the disease for many years. The recently released tobacco cultivar, Coker 371-Gold (C 371-G), was found to provide an additiona ...
DOI:
10.1094/PDIS.1997.81.11.1269

75. Inhibition of trehalase activity enhances trehalose accumulation in transgenic plants

Author:
Goddijn, O.J.M.; Verwoerd, T.C.; Voogd, E.; Krutwagen, R.W.H.H.; Graaf, P.T.H.M. de.; Poels, J.; Dun, K. van.; Ponstein, A.S.; Dammn, B.; Pen, J.
Source:
Plant physiology 1997 v.113 no.1 pp. 181-190
ISSN:
0032-0889
Subject:
Nicotiana tabacum; Solanum tuberosum; tubers; leaves; trehalose; biosynthesis; quantitative analysis; hexosyltransferases; phosphoric monoester hydrolases; trehalase; enzyme activity; enzyme inhibitors; plant development; genetic engineering; transgenic plants; genes; gene transfer; gene expression; cultivars; genetic variation
Abstract:
... As a first step toward the exploitation of the disaccharide trehalose as a stress-protective and preservative agent in plants, we engineered trehalose biosynthesis in tobacco (Nicotiana tabacum) and potato (Solanum tuberosum) by introducing the otsA and otsB genes from Escherichia coli, which encode trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase, respectively. In leaves of tr ...
PubMed:
9008394
PubMed Central:
PMC158129

76. Integration of T-DNA binary vector 'backbone' sequences into the tobacco genome: evidence for multiple complex patterns of integration

Author:
Kononov, M.E.; Bassuner, B.; Gelvin, S.B.
Source:
plant journal 1997 v.11 no.5 pp. 945-957
ISSN:
0960-7412
Subject:
genetic transformation; transgenic plants; Agrobacterium radiobacter; plasmid vectors; gene expression; Nicotiana tabacum; reporter genes; beta-glucuronidase
Abstract:
... During the process of crown gall tumorigenesis, Agrobacterium tumefaciens transfers part of the tumor-inducing (Ti) plasmid, the T-DNA, to a plant cell where it eventually becomes stably integrated into the plant genome. Directly repeated DNA sequences, called T-DNA borders, define the left and the right ends of the T-DNA. The T-DNA can be physically separated from the remainder of the Ti-plasmid, ...
DOI:
10.1046/j.1365-313X.1997.11050945.x
PubMed:
9193068

77. Interaction of resistant plants and wasp parasitoids of tobacco budworm (Lepidoptera: Noctuidae)

Author:
Johnson, M.T.
Source:
Environmental entomology 1997 v.26 no.2 pp. 207-214
ISSN:
0046-225X
Subject:
parasitism; parasitoids; transgenic plants; biological control agents; Bacillus thuringiensis; Nicotiana tabacum; mortality; toxins; synergism; Heliothis virescens; Campoletis sonorensis; pest resistance; Cardiochiles nigriceps; biological control; North Carolina
Abstract:
... The interactions of 2 species of parasitoid wasps, their host, Heliothis virescens (F.), and transgenic tobacco producing a low concentration of Bacillus thuringiensis Berliner toxin were investigated in field tests. Campoletis sonorensis (Cameron) and toxic plants each decreased survival of larvae during the first 6 d on plants. C. sonorensis and toxic plants acted synergistically in combination, ...
DOI:
10.1093/ee/26.2.207

78. Interactions between the biocontrol agent Pseudomonas fluorescens CHA0 and Thielaviopsis basicola in tobacco roots observed by immunofluorescence microscopy

Author:
Troxler, J.; Berling, C.H.; Moenne-Loccoz, Y.; Keel, C.; Defago, G.
Source:
Plant pathology 1997 v.46 no.1 pp. 62-71
ISSN:
0032-0862
Subject:
Thielaviopsis basicola; Pseudomonas fluorescens; Nicotiana tabacum; fluorescent antibody technique; seedlings; cortex; xylem; mycelium; biological control; disease course; immunohistochemistry; disease control; plant diseases and disorders
DOI:
10.1046/j.1365-3059.1997.d01-205.x

79. Involvement of thylakoid overenergization in tentoxin-induced chlorosis in Nicotiana spp

Author:
Holland, N.; Evron, Y.; Jansen, M.A.K.; Edelman, M.; Pick, U.
Source:
Plant physiology 1997 v.114 no.3 pp. 887-892
ISSN:
0032-0889
Subject:
seedlings; plant pathogenic fungi; Nicotiana plumbaginifolia; dose response; Nicotiana tabacum; Alternaria alternata; chlorosis; oxygen; adenosinetriphosphatase; superoxide dismutase; adenosine triphosphate; pathogenicity; enzyme activity; gramicidin; ascorbic acid; biosynthesis; free radicals; thylakoids; transgenic plants; photophosphorylation; mutants
Abstract:
... The purpose of this work was to clarify the mechanism of tentoxin-induced chlorosis inNicotiana spp. seedlings. We found that chlorosis does not correlate with the inhibition of chloroplast ATP synthesis in vivo, since it occurs at tentoxin concentrations far higher than that required for the inhibition of photophosphorylation measured in the same seedlings. However, tentoxin-induced chlorosis doe ...

80. Isolation and characterization of cDNA clones with enhanced expression in tobacco plants expressing the rice homeobox gene, OSH1

Author:
Tamaoki, M.; Yamamoto, N.; Matsuoka, M.
Source:
Plant & cell physiology 1997 v.38 no.5 pp. 638-642
ISSN:
0032-0781
Subject:
complementary DNA; Oryza sativa; transgenic plants; clones; gene expression; genes; Nicotiana tabacum; nucleotide sequences
Abstract:
... We have used subtractive hybridization to isolate cDNA clones whose expression were up-regulated in transgenic tobacco ectopically expressing the rice homeobox gene, OSH1. Thirty-nine distinct cDNA clones, which we term HRGs (Homeobox Regulated Genes), were identified. Some of them were specifically expressed in transformants, indicating that their expression was possibly regulated by transgene. ...
DOI:
10.1093/oxfordjournals.pcp.a029215
PubMed:
9210336

81. Light-induced transcriptional repression of the pea AS1 gene: identification of cis-elements and transfactors

Author:
Ngai, N.; Tsai, F.Y.; Coruzzi, G.
Source:
plant journal 1997 v.12 no.5 pp. 1021-1034
ISSN:
0960-7412
Subject:
transcription factors; transgenic plants; plasmid vectors; transcription (genetics); chromosome mapping; Nicotiana tabacum; DNA-binding proteins; reporter genes; Pisum sativum; aspartate-ammonia ligase; promoter regions; beta-glucuronidase; light
Abstract:
... Here, we examine the cis-elements and trans-factors affecting the expression of asparagine synthetase (AS) genes whose transcription is negatively regulated by light. The promoters for the AS1 and AS2 genes of pea were isolated, sequenced, and functionally dissected for their ability to confer regulated expression to the GUS reporter gene in transgenic tobacco. Histochemical analysis of transgenic ...
DOI:
10.1046/j.1365-313X.1997.12051021.x

82. Light-regulated expression of the pea plastocyanin gene is mediated by elements within the transcribed region of the gene

Author:
Helliwell, C.A.; Webster, C.I.; Gray, J.C.
Source:
plant journal 1997 v.12 no.3 pp. 499-506
ISSN:
0960-7412
Subject:
messenger RNA; ribosomal RNA; transgenic plants; gene expression; Nicotiana tabacum; reporter genes; Pisum sativum; promoter regions; beta-glucuronidase; light
Abstract:
... Expression of the pea plastocyanin gene (PetE) is regulated by light in both pea and transgenic tobacco plants. However, the PetE promoter with the 5' untranslated leader region does not direct light-regulated expression of the GUS reporter gene in transgenic tobacco. This suggested that sequences downstream of the translation start of the PetE gene are required for light-regulated expression. To ...
DOI:
10.1046/j.1365-313X.1997.00499.x
PubMed:
9351238

83. Lipopeptide phytotoxins produced by Pseudomonas syringae pv. syringae: comparison of the biosurfactant and ion channel-forming activities of syringopeptin and syringomycin

Author:
Hutchison, M.L.; Gross, D.C.
Source:
Molecular plant-microbe interactions 1997 v.10 no.3 pp. 347-354
ISSN:
0894-0282
Subject:
Pseudomonas syringae pv. syringae; bacterial toxins; peptides; cytolysis; protoplasts; membrane permeability; Nicotiana tabacum; surfactants; erythrocytes; inorganic ions; calcium; plasma membrane; ion channels
Abstract:
... The phytopathogenic bacterium Pseudomonas syringae pv. syringae produces two classes of necrosis-inducing lipodepsipeptide toxins commonly referred to as the syringomycins and syringopeptins. Members of the syringomycin class are pore-forming cytotoxins that act by promoting passive transmembrane ion flux. In this study, we test the hypothesis that syringopeptin forms SP22A and SP22B likewise func ...
DOI:
10.1094/MPMI.1997.10.3.347
PubMed:
9100379

84. Local and systemic responses to antioxidants to tobacco mosaic virus infection and to salicylic acid to tobacco. Role in systemic acquired resistance

Author:
Fodor, J.; Gullner, G.; Adam, A.L.; Barna, B.; Komives, T.; Kiraly, Z.
Source:
Plant physiology 1997 v.114 no.4 pp. 1443-1451
ISSN:
0032-0889
Subject:
dehydroascorbic acid; necrosis; salicylic acid; Tobacco mosaic virus; glutathione; dose response; glutathione transferase; Nicotiana tabacum; disease resistance; leaves; quantitative analysis; superoxide dismutase; pathogenicity; enzyme activity; isozymes; antioxidants; ascorbic acid; glutathione-disulfide reductase; catalase; chemical constituents of plants
Abstract:
... Changes in ascorbate and glutathione levels and in activitiesof ascorbate peroxidase, catalase, dehydroascorbate reductase (DHAR), glutathione reductase (GR), glutathione S-transferase (GST), and superoxide dismutase (SOD) were investigated in tobacco mosaic virus (TMV)-inoculated lower leaves and in noninoculated upper leaves of Nicotiana tabacum L. cv Xanthi-nc. In separate experiments the effec ...

85. Localization of a kinesin-like calmodulin-binding protein in dividing cells of Arabidopsis and tobacco

Author:
Bowser, J.; Reddy, A.S.N.
Source:
plant journal 1997 v.12 no.6 pp. 1429-1437
ISSN:
0960-7412
Subject:
Arabidopsis thaliana; calcium; fluorescence; cell division; cultured cells; Nicotiana tabacum; microtubules; chemical constituents of plants; molecular weight; immunocytochemistry; calmodulin; plant proteins
Abstract:
... The cloning and characterization of a novel kinesin-like protein (kinesin-like calmodulin-binding protein, KCBP) from Arabidopsis and other plants has recently been described. Unlike all other known kinesin-like proteins, KCBP interacts with calmodulin in the presence of micro-molar calcium. An antibody specific to KCBP was raised using a calmodulin-binding synthetic peptide that is unique to KCBP ...
DOI:
10.1046/j.1365-313x.1997.12061429.x
PubMed:
9450347

86. Mannitol protects against oxidation by hydroxyl radicals

Author:
Shen, B.; Jensen, R.G.; Bohnert, H.J.
Source:
Plant physiology 1997 v.115 no.2 pp. 527-532
ISSN:
0032-0889
Subject:
drought; mannitol; phosphotransferases (kinases); betaine; metabolites; glutathione; Nicotiana tabacum; oxidation; Calvin cycle; superoxide dismutase; enzyme inhibitors; enzyme activity; ribulose-bisphosphate carboxylase; free radicals; thylakoids; catalase; cooling; stress response; phenylalanine
Abstract:
... Hydroxyl radicals may be responsible for oxidative damage during drought or chilling stress. We have shown that the presence of mannitol in chloroplasts can protect plants against oxidative damage by hydroxyl radicals (B. Shen, R.G. Jensen, H.J. Bohnert [1997] Plant Physiol 113:1177-1183). Here we identify one of the target enzymes that may be protected by mannitol. Isolated thylakoids in the pres ...

87. Metabolization of the artificial secondary metabolite 4-hydroxybenzoate in ubiC-transformed tobacco

Author:
Li, S.M.; Wang, Z.X.; Wemakor, E.; Heide, L.
Source:
Plant & cell physiology 1997 v.38 no.7 pp. 844-850
ISSN:
0032-0781
Subject:
cell wall components; biosynthesis; lyases; pyruvic acid; genetic code; transgenic plants; benzoates; secondary metabolites; gene expression; cultured cells; Nicotiana tabacum; glucosides; pH; genes; transferases
Abstract:
... Transgenic Nicotiana tabacum cell cultures formed a new, artificial secondary metabolite, i.e. 4-hydroxybenzoate (4HB), by expression of the bacterial gene ubiC, which encodes chorismate pyruvate-lyase. 4HB was converted in the cells to two different glucosides, i.e. 4-O-(1-beta-D- glucosyl)benzoic acid (4HBOG) and 4HB 1-beta-D-glucosyl ester (4HBCOOG). The same metabolization was also observed wi ...
DOI:
10.1093/oxfordjournals.pcp.a029243

88. Methyl jasmonate exposure induces insect resistance in cabbage and tobacco

Author:
Avdiushko, S.A.; Brown, G.C.; Dahlaman, D.L.; Hildebrand, D.F.
Source:
Environmental entomology 1997 v.26 no.3 pp. 642-654
ISSN:
0046-225X
Subject:
Trichoplusia ni; Manduca sexta; jasmonic acid; enzyme activity; linoleate 13S-lipoxygenase; Nicotiana tabacum; feeding behavior; induced resistance; biological development; Brassica oleracea; pest resistance; aldehydes
Abstract:
... The effect of methyl jasmonate treatment of 2 plant species (cabbage and tobacco) on the feeding of their corresponding lepidopteran pests was studied. Treatment of cabbage and tobacco ants with methyl jasmonate increased lipoxygenase activity in the leaf tissues. Reduced feeding of lepidopteran larvae was observed in choice test experiments with cabbage loopers and tobacco hornworms. Similar resu ...
DOI:
10.1093/ee/26.3.642

89. Micromanipulation of male and female gametes of Nicotiana tabacum. I. Isolation of gametes

Author:
Tian, H.Q.; Russell, S.D.
Source:
Plant cell reports 1997 v.16 no.8 pp. 555-560
ISSN:
0721-7714
Subject:
protoplasts; ovules; in vitro culture; germ cells; enzymes; Nicotiana tabacum; micropropagation; pollen; culture media
Abstract:
... The isolation of male and female gametes is a precondition for the micromanipulation of flowering plant gametes. To reflect their condition at fertilization, isolated gametes need to he physiologically mature and vigorous. Sperm cells are isolated from pollen tubes grown on cut styles using the "in vivo/in vitro" technique. Embryo sacs are isolated 2 days after anthesis using brief treatments of m ...
DOI:
10.1007/s002990050278

90. Modeling dose-response relationships in biological control: partitioning host responses to the pathogen and biocontrol agent

Author:
Smith, K.P.; Handelsman, J.; Goodman, R.M.
Source:
Phytopathology 1997 v.87 no.7 pp. 720-729
ISSN:
0031-949X
Subject:
Cucumis sativus; Allium sativum; Arabidopsis thaliana; Lolium perenne; Solanum lycopersicum var. lycopersicum; Medicago sativa; Nicotiana tabacum; Zea mays; Pythium; plant pathogenic fungi; pathogenicity; host-parasite relationships; Bacillus cereus; inoculum density; biological control; disease resistance; plant breeding; ecotypes; cultivars; genetic variation; statistical analysis; mathematical models; equations; disease control; plant diseases and disorders
Abstract:
... Breeding plants to improve the effectiveness of biocontrol agents is a promising approach to enhance disease suppression by microorganisms. Differences in biocontrol efficacy among cultivars suggest there is genetic variation for this trait within crop germplasm. The ability to quantify host differences in support of biological control is influenced by variation in host response to the pathogen an ...
DOI:
10.1094/PHYTO.1997.87.7.720
PubMed:
18945094

91. Molecular characterization of a bean alpha-amylase inhibitor that inhibits the alpha-amylase of the Mexican bean weevil Zabrotes subfasciatus

Author:
Grossi de Sa, M.F.; Mirkov, T.E.; Ishimoto, M.; Colucci, G.; Bateman, K.S.; Chrispeels, M.J.
Source:
Planta 1997 v.203 no.3 pp. 295-303
ISSN:
0032-0935
Subject:
pancreas; seeds; complementary DNA; Zabrotes subfasciatus; transgenic plants; animal proteins; Phaseolus vulgaris; enzyme inhibitors; gene expression; amino acid sequences; pH; Nicotiana tabacum; swine; binding sites; enzyme activity; alpha-amylase; plant proteins
Abstract:
... Cultivated varieties of the common bean (Phaseolus vulgaris L.) contain an alpha-amylase inhibitor (alpha AI-1) that inhibits porcine pancreatic alpha-amylase (PPA; EC 3.2.1.1) and the amylases of certain seed weevils, but not that of the Mexican bean weevil Zabrotes subfasciatus. A variant of alpha AI-1, called alpha AI-2, is found in certain arcelin-containing wild accessions of the common bean. ...
DOI:
10.1007/s004250050195
PubMed:
9431678

92. Molecular cloning and further characterization of a portable plant vacuolar sorting receptor

Author:
Paris, N.; Rogers, S.W.; Jiang, L.W.; Kirsch, T.; Beevers, L.; Phillips, T.E.; Rogers, J.C.
Source:
Plant physiology 1997 v.115 no.1 pp. 29-39
ISSN:
0032-0889
Subject:
Oryza sativa; vacuoles; cell membranes; genes; Pisum sativum; proline; binding proteins; gene expression; Nicotiana tabacum; protein composition; Arabidopsis thaliana; Zea mays; complementary DNA; arginine; amino acid sequences; isoleucine; immunocytochemistry; Golgi apparatus; asparagine; cell suspension culture
Abstract:
... BP-80 is a type I integral membrane protein abundant in pea (Pisum sativum) clathrin-coated vesicles (CCVs) that binds with high affinity to vacuole-targeting determinants containing asparagine-proline-isoleucine-arginine. Here we present results from cDNA cloning and studies of its intracellular localization. Its sequence and sequences of homologs from Arabidopsis, rice (Oryza sativa), and maize ...

93. Monoclonal antibodies to barley aleurain and homologs from other plants

Author:
Rogers, S.W.; Burks, M.; Rogers, J.C.
Source:
plant journal 1997 v.11 no.6 pp. 1359-1368
ISSN:
0960-7412
Subject:
Arabidopsis thaliana; cysteine proteinases; acidity; root tips; Petunia hybrida; Hordeum vulgare; vacuoles; isozymes; plant extracts; monoclonal antibodies; amino acid sequences; Nicotiana tabacum; molecular weight; immunocytochemistry; fluorescent antibody technique; plant proteins
Abstract:
... Barley aleurain is contained within a specific type of vacuole characterized by acidic pH and the presence of other hydrolytic enzymes. The aleurain-containing vacuole is distinct from protein storage vacuoles, and anti-aleurain antibodies serve as markers for this organelle in barley cells. Aleurain is a unique type of cysteine protease, and other plant species have genes for homologs whose seque ...
DOI:
10.1046/j.1365-313X.1997.11061359.x
PubMed:
9225474

94. Multi-element and multi-site isotopic analysis of nicotine from tobacco leaves

Author:
Jamin, E.; Naulet, N.; Martin, G.J.
Source:
Plant, cell and environment 1997 v.20 no.5 pp. 589-599
ISSN:
0140-7791
Subject:
carbon; stable isotopes; nicotine; Nicotiana tabacum; leaves; metabolism; provenance; deuterium; chemical composition; pyrrolidine alkaloids; nitrogen; biochemical pathways; niacin; United States; Philippines; India; Thailand; Indonesia; Cuba; Europe; South America; China; Africa
Abstract:
... The carbon, nitrogen and hydrogen stable-isotope contents of nicotine extracted from tobacco leaves were determined by Isotope Ratio Mass Spectrometry (overall 2H, 13C and 15N contents) and by the SNIF-NMR method (site-specific deuterium content). In addition, nicotine was chemically degraded into nicotinic acid so that the intramolecular distribution of carbon and nitrogen isotopes could be studi ...
DOI:
10.1111/j.1365-3040.1997.00099.x

95. Mutation of the tobacco mosaic tobamovirus 126- and 183-kDa proteins: effects on phloem-dependent virus accumulation and synthesis of viral proteins

Author:
Derrick, P.M.; Carter, S.A.; Nelson, R.S.
Source:
Molecular plant-microbe interactions 1997 v.10 no.5 pp. 589-596
ISSN:
0894-0282
Subject:
coat proteins; protoplasts; Tobacco mosaic virus; protein synthesis; phenotype; stems; immunohistochemistry; mutation; amino acid sequences; phloem; Nicotiana tabacum; amino acids; mutants; molecular weight; virulence
Abstract:
... The masked and U1 strains of tobacco mosaic tobamovirus differ in symptom phenotype and in phloem-dependent accumulation in tobacco. The symptom phenotype is determined by eight amino acids in the 126- and 183-kDa proteins that differ between the two strains. In this study, slow phloem-dependent accumulation of the masked strain was shown to be determined by these same eight amino acids, but some ...
DOI:
10.1094/MPMI.1997.10.5.589

96. Patterns of phenolic compounds in leafy galls of tobacco

Author:
Verrecke, D.; Messens, E.; Klarskov, K.; Bruyn, A.; Bruyn, A. de.; Montagu, M. van.; Goethals, K.
Source:
Planta 1997 v.201 no.3 pp. 342-348
ISSN:
0032-0935
Subject:
metabolism; caffeic acid; Rhodococcus fascians; chemical constituents of plants; Agrobacterium radiobacter; cell division; cinnamic acid; Nicotiana tabacum; growth retardation; coumarins; spectral analysis; chlorogenic acid; galls; virulence
Abstract:
... The chemical composition of ethanolic and aqueous extracts from leafy galls produced after infection of Nicotiana tabacum L. plants with Rhodococcus fascians was drastically changed compared to uninfected controls. Chlorogenic acid was abundant both in uninfected and infected plants, but caffeic acid and another cinnamoyl analogue were new in leafy galls. The most pronounced product induced in lea ...
DOI:
10.1007/s004250050076
PubMed:
19343412

97. Phenotypic characterization of a tobacco mutant impaired in auxin polar transport

Author:
Naderi, M.; Caplan, A.; Berger, P.H.
Source:
Plant cell reports 1997 v.17 no.1 pp. 32-38
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; nutrient transport; phenotype; Agrobacterium radiobacter; embryo (plant); developmental stages; cultured cells; Nicotiana tabacum; mutants; abnormal development; endosperm; genetic variation; ultrastructure; plant morphology; indole acetic acid; seed development
Abstract:
... A mutation in tobacco (Nicotiana tabacum L. cv 'Xanthi') called lat (low auxin transport) that changes many morphogenic features throughout the life of the plant has been isolated. Abnormalities were observed in seed development, embryogenesis, cotyledon formation, leaf initiation and development, leaf veination pattern, and flower development. Selfed R2 lat mutant plants set between 60% and 90% f ...
DOI:
10.1007/s002990050347

98. Phenotypic variation in transgenic tobacco expressing mutated geminivirus movement/pathogenicity (BC1) proteins

Author:
Duan, Y.P.; Powell, C.A.; Purcifull, D.E.; Broglio, P.; Hiebert, E.
Source:
Molecular plant-microbe interactions 1997 v.10 no.9 pp. 1065-1074
ISSN:
0894-0282
Subject:
Nicotiana tabacum; transgenic plants; Geminiviridae; viral proteins; pathogenicity; gene expression; growth retardation; nucleotide sequences; amino acid sequences; phenotype; mutation; genetic recombination
Abstract:
... Tobacco plants were transformed with the movement protein (pathogenicity) gene (BC1) from tomato mottle geminivirus (TMoV), using Agrobacterium-mediated transformation. Different transgenic tobacco lines that expressed high levels of the BC1 protein had phenotypes ranging from plants with severe stunting and leaf mottling (resembling geminivirus symptoms) to plants with no visible symptoms. The se ...
DOI:
10.1094/MPMI.1997.10.9.1065
PubMed:
9390421

99. Photoautotrophic tobacco cells adapted to grow at high salinity

Author:
Chang, C.C.; Locy, R.D.; Smeda, R.; Sahi, S.V.; Singh, N.K.
Source:
Plant cell reports 1997 v.16 no.7 pp. 495-502
ISSN:
0721-7714
Subject:
salt tolerance; dose response; quantitative analysis; salinity; glucose; cell culture; sucrose; thylakoids; net assimilation rate; starch; cultured cells; Nicotiana tabacum; sodium chloride; fructose; photosynthesis; protein content; culture media
Abstract:
... Photoautotrophic tobacco (Nicotiana tabacum var. Wisconsin 38) cell cultures were gradually adapted to grow in media containing the normally inhibitory concentration of 20 g l-1 NaCl. Both salt-adapted cultures maintained in 20 g l-1 NaCl (P20) and salt-unadapted (P0) cultures demonstrated similar chloroplast morphology and similar growth characteristics on a dry weight basis, but P20 cells showed ...
DOI:
10.1007/s002990050267

100. Phytochrome-induced intercellular signalling activates cab::luciferase gene expression

Author:
Bischoff, F.; Millar, A.J.; Kay, S.A.; Furuya, M.
Source:
plant journal 1997 v.12 no.4 pp. 839-849
ISSN:
0960-7412
Subject:
Nicotiana tabacum; phytochrome; gene expression; transgenic plants; luciferase; reporter genes; cotyledons; red light; light intensity; lighting; histochemistry; photoreceptors; promoter regions
Abstract:
... The phytochrome-induced expression pattern of the chlorophyll a/b binding protein (cab) gene was studied using a cab2::luciferase reporter transgene in the tobacco cotyledon. The role of developmentally regulated competence, cooperativity among cells and signal propagation was investigated by red-light microbeam irradiation of distinct areas of the cotyledon. Even with a minimal fluence, the respo ...
DOI:
10.1046/j.1365-313X.1997.12040839.x