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- Haggman, H.M.; Aronen, T.S.; Nikkanen, T.O.
- Canadian journal of forest research = 1997 v.27 no.6 pp. 928-935
- Pinus sylvestris; Picea abies; transgenic plants; pollen; beta-glucuronidase; gene expression; Helianthus annuus; biolistics; pollination; genetic transformation; promoter regions
- ... The aim of the present work was to genetically transform Scots pine (Pinus sylvestris L.) and Norway spruce (Picea abies (L.) Karst.) pollen in order to study the possibilities of obtaining transgenic progenies. The effects of different pollen origins and regulatory sequences on the beta-glucuronidase (GUS) expression in pollen were significant. The highest GUS expression in pine (55 +/- 7.7% of g ...
- Holbrook, L.; Tung, P.; Ward, K.; Reid, D.M.; Abrams, S.; Lamb, N.; Quail, J.W.; Moloney, M.M.
- Plant physiology 1997 v.114 no.2 pp. 419-428
- Helianthus annuus; Brassica napus; measurement; chemical degradation; dose response; jasmonic acid; gene expression; Triticum aestivum; Nicotiana tabacum; esters; stereochemistry; enzyme activity; chemical structure; seed germination; transgenic plants; cotyledons; proteinase inhibitors; reporter genes; chemical constituents of plants; chlorophyll; beta-glucuronidase
- ... The importance of the two chiral centers at C-3 and C-7 in the molecular structure of jasmonic acid in plant responses was investigated. We separated methyl jasmonate (MeJA) into (3R)- and (3S)-isomers with a fixed stereochemistry at C-3, but epimerization at C-7 is possible. The four isomers of the nonepimerizable analog 7-methyl MeJA were synthesized. These six esters and their corresponding aci ...