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You searched for: Publication Year 1997 Remove constraint Publication Year: 1997 Subject beta-glucuronidase Remove constraint Subject: beta-glucuronidase
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1. A 146 bp fragment of the tobacco Lhcb1*2 promoter confers very-low-fluence, low-fluence and high-irradiance responses of phytochrome to a minimal CaMV 35S promoter

Author:
Pablo D. Cerdán; Roberto J. Staneloni; Jorge J. Casal; Rodolfo A. Sánchez
Source:
Plant molecular biology 1997 v.33 no.2 pp. 245-255
ISSN:
0167-4412
Subject:
Cauliflower mosaic virus; Nicotiana tabacum; beta-glucuronidase; far-red light; gene expression; genes; light intensity; phytochrome; promoter regions; red light; seedlings; tobacco; transgenic plants
Abstract:
... The occurrence of very-low-fluence responses (VLFR), low-fluence responses (LFR) and high-irradiance responses (HIR) of phytochrome was investigated for the expression of the gene of β-glucuronidase (gusA) under the control of the tobacco Lhcb1*2 promoter, in etiolated transgenic tobacco seedlings. The activity of β-glucuronidase (GUS) showed biphasic responses to the calculated proportion of Pf ...
DOI:
10.1023/A:1005784407282

2. Agrobacterium tumefaciens-mediated barley transformation

Author:
Tingay, S.; McElroy, D.; Kalla, R.; Fieg, S.; Wang, M.B.; Thornton, S.; Brettell, R.
Source:
plant journal 1997 v.11 no.6 pp. 1369-1376
ISSN:
0960-7412
Subject:
genetic transformation; phosphinothricin acetyltransferase; Hordeum vulgare; transgenic plants; embryo (plant); Agrobacterium radiobacter; plasmid vectors; explants; gene expression; glufosinate; progeny; reporter genes; herbicide resistance; enzyme activity; bilanafos; beta-glucuronidase
Abstract:
... Genetically transformed barley was produced by cocultivating immature embryo explants with Agrobacterium tumefaciens carrying a binary vector coding for chimaeric bacterial genes, bar and gus, and selecting for bialaphos-resistant cultures from which plants were regenerated. Integration of both genes was confirmed by gel blot hybridization analysis of DNA from the transformed plants and their prog ...
DOI:
10.1046/j.1365-313X.1997.11061369.x

3. Agrobacterium-mediated transformation of the terpenoid indole alkaloid-producing plant species Tabernaemontana pandacaqui

Author:
Lopes-Cardoso, M.I.; Meijer, A.H.; Hoge, J.H.C.
Source:
Plant cell reports 1997 v.17 no.2 pp. 150-154
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; terpenoids; Catharanthus roseus; biosynthesis; transgenic plants; Agrobacterium radiobacter; gene expression; indole alkaloids; reporter genes; Tabernaemontana pandacaqui; catalytic activity; beta-glucuronidase
Abstract:
... Plants of the Apocynaceae family produce a wide range of terpenoid indole alkaloids (TIAs) which have important pharmaceutical applications. Studies of the molecular mechanisms controlling TIA biosynthesis may eventually provide possibilities to improve product yield by genetic modification of plants or cell cultures. However, these studies suffer from the lack of transformation/regeneration proto ...
DOI:
10.1007/s002990050369

4. Analysis of SCARECROW expression using a rapid system for assessing transgene expression in Arabidopsis roots

Author:
Malamy, J.E.; Benfey, P.N.
Source:
plant journal 1997 v.12 no.4 pp. 957-963
ISSN:
0960-7412
Subject:
Arabidopsis thaliana; gene expression; roots; transgenic plants; callus; mutants; genetic transformation; reporter genes; beta-glucuronidase; promoter regions; histochemistry
Abstract:
... Although the introduction of foreign genes into Arabidopsis has become routine, the production of transgenic Arabidopsis plants still requires several months. A transgene expression system (TES) has been developed that allows characterization of gene expression patterns and the effects of foreign genes in the Arabidopsis root in 2-4 weeks. The method is based on regeneration of stably transformed ...
DOI:
10.1046/j.1365-313X.1997.12040957.x
PubMed:
9375406

5. The Arabidopsis MALE STERILITY 2 protein shares similarity with reductases in elongation/condensation complexes

Author:
Aarts, M.G.M.; Hodge, R.; Kalantidis, K.; Florack, D.; Wilson, Z.A.; Mulligan, B.J.; Stiekema, W.J.; Scott, R.; Pereira, A.
Source:
plant journal 1997 v.12 no.3 pp. 615-623
ISSN:
0960-7412
Subject:
fatty acids; flowering; pollen; promoter regions; plant proteins; cell walls; gene expression; Nicotiana tabacum; cell wall components; Arabidopsis thaliana; fatty alcohols; spores; amino acid sequences; histochemistry; transgenic plants; oxidoreductases; reporter genes; beta-glucuronidase
Abstract:
... The Arabidopsis thaliana MALE STERILITY 2 (MS2) gene product is involved in male gametogenesis. The first abnormalities in pollen development of ms2 mutants are seen at the stage in microsporogenesis when microspores are released from tetrads. Expression of the MS2 gene is observed in tapetum of wild-type flowers at, and shortly after, the release of microspores from tetrads. The MS2 promoter cont ...
DOI:
10.1046/j.1365-313X.1997.00615.x
PubMed:
9351246

6. AtDMC1, the Arabidopsis homologue of the yeast DMC1 gene: characterization, transposon-induced allelic variation and meiosis-associated expression

Author:
Klimyuk, V.I.; Jones, J.D.G.
Source:
plant journal 1997 v.11 no.1 pp. 1-14
ISSN:
0960-7412
Subject:
alleles; pollen; promoter regions; transposons; gene expression; introns; Arabidopsis thaliana; ovules; messenger RNA; spores; complementary DNA; amino acid sequences; meiosis; exons; histochemistry; flowers; genetic variation; beta-glucuronidase
Abstract:
... Based on homologies between the yeast DMC1 and the lily LIM15 meiosis-specific genes, degenerate PCR primers were designed that amplified the Arabidopsis DMC1 gene (AtDMC1). AtDMC1 genomic DNA (8 kb) was sequenced, and the transcript was characterized by reverse transcriptase-polymerase chain reaction (RT-PCR) and by 5' and 3' RACE (rapid amplification of cDNA ends). The AtDMC1 gene contains 15 ex ...
DOI:
10.1046/j.1365-313X.1997.11010001.x
PubMed:
9025299

7. Cell-specific expression of the promoters of two nonlegume hemoglobin genes in a transgenic legume, Lotus corniculatus

Author:
Andersson, C.R.; Llewellyn, D.J.; Peacock, W.J.; Dennis, E.S.
Source:
Plant physiology 1997 v.113 no.1 pp. 45-57
ISSN:
0032-0889
Subject:
developmental stages; promoter regions; Parasponia; nitrogen fixation; gene expression; Casuarina glauca; Lotus corniculatus; leghemoglobin; root nodules; amino acid sequences; transcription (genetics); Rhizobium; enzyme activity; histochemistry; Trema; gene transfer; symbiosis; transgenic plants; hemoglobin; reporter genes; beta-glucuronidase
Abstract:
... The promoters of the hemoglobin genes from the nitrogen-fixing tree Parasponia andersonii and the related nonnitrogen-fixing Trema tomentosa both confer beta-glucuronidase reporter gene expression to the central zone of the nodules of a transgenic legume, Lotus corniculatus. beta-Glucuronidase expression was high in the uninfected interstitial cells and parenchyma of the surrounding boundary layer ...
PubMed:
9008386
PubMed Central:
PMC158114

8. Cell-to-cell trafficking of cucumber mosaic virus movement protein:green fluorescent protein fusion produced by biolistic gene bombardment in tobacco

Author:
Itaya, A.; Hickman, H.; Bao, Y.; Nelsen, R.; Ding, B.
Source:
plant journal 1997 v.12 no.5 pp. 1223-1230
ISSN:
0960-7412
Subject:
Nicotiana tabacum; Cucumber mosaic virus; viral proteins; biolistics; transgenic plants; leaves; reporter genes; plasmodesmata; beta-glucuronidase; animal proteins; recombinant proteins; Scyphozoa; translocation (plant physiology)
Abstract:
... Previous micro-injection studies showed that some recombinant viral movement proteins and plant proteins produced in and purified from Escherichia coli could traffic from cell to cell. However, the relevance of these findings obtained by micro-injecting proteins produced in E. coli to the real functions of these proteins when produced in planta has been questioned. In this study, specific gene con ...
DOI:
10.1046/j.1365-313X.1997.12051223.x

9. Changes in targeting efficiencies of proteins to plant microbodies caused by amino acid substitutions in the carboxy-terminal tripeptide

Author:
Hayashi, M.; Aoki, M.; Kondo, M.; Nishimura, M.
Source:
Plant & cell physiology 1997 v.38 no.6 pp. 759-768
ISSN:
0032-0781
Subject:
Arabidopsis thaliana; cotyledons; leaves; cytosol; peptides; protein transport; glyoxysomes; peroxisomes; beta-glucuronidase; transgenic plants; binding sites
Abstract:
... It has been demonstrated that the carboxyl terminus of microbody enzymes functions as a targeting signal to microbodies in higher plants. We have examined an ability of 24 carboxy-terminal amino acid sequences to facilitate the transport of a cytosolic passenger protein, beta-glucuronidase, into microbodies in green cotyledonary cells of transgenic Arabidopsis. Immunoelectron microscopic analysis ...
DOI:
10.1093/oxfordjournals.pcp.a029233
PubMed:
9249991

10. Characterization of proteins that interact with the GTP-bound form of the regulatory GTPase Ran in Arabidopsis

Author:
Haizel, T.; Merkle, T.; Pay, A.; Fejes, E.; Nagy, F.
Source:
plant journal 1997 v.11 no.1 pp. 93-103
ISSN:
0960-7412
Subject:
Arabidopsis thaliana; guanosinetriphosphatase; phenotype; complementary DNA; pyrophosphatases; transgenic plants; meristems; guanosine triphosphate; introns; binding proteins; gene expression; amino acid sequences; exons; molecular weight; cell cycle; beta-glucuronidase; plant proteins
Abstract:
... Ran, a small soluble GTP-binding protein, has been shown to be essential for the nuclear translocation of proteins and it is also thought to be involved in regulating cell cycle progression in mammalian and yeast cells. Genes encoding Ran-like proteins have been isolated from different higher plant species. Overexpression of plant Ran cDNAs, similarly to their mammalian/yeast homologues, suppresse ...
DOI:
10.1046/j.1365-313X.1997.11010093.x
PubMed:
9025305

11. Characterization of the targeted nuclear accumulation of GFP within the cells of transgenic plants

Author:
Grebenok, R.J.; Lambert, G.M.; Galbraith, D.W.
Source:
plant journal 1997 v.12 no.3 pp. 685-696
ISSN:
0960-7412
Subject:
Nicotiana tabacum; transgenic plants; cytoplasm; active transport; molecular weight; amino acid sequences; plant proteins; cytochemistry; spatial distribution; leaves; beta-glucuronidase; enzyme activity; animal proteins; Scyphozoa; green fluorescent protein; Aequorea victoria
Abstract:
... The soluble proteins of the nucleoplasm are synthesized on cytoplasmic ribosomes. Proteins larger than about 40 kDa are post-translationally targeted to the nucleus via energy-dependent processes, passing through the nuclear pore complex into the nucleoplasm. Targeting involves nuclear localization signals (NLSs) found within the primary sequences of the imported proteins. In higher plants, inform ...
DOI:
10.1046/j.1365-313X.1997.00685.x

12. Cinnamyl alcohol dehydrogenase: identification of new sites of promoter activity in transgenic poplar

Author:
Hawkins, S.; Samaj, J.; Lauvergeat, V.; Boudet, A.; Grima-Pettenati, J.
Source:
Plant physiology 1997 v.113 no.2 pp. 321-325
ISSN:
0032-0889
Subject:
alcohol dehydrogenase; gene transfer; promoter regions; hybrids; stems; Populus alba; transgenic plants; Eucalyptus gunnii; cinnamic acid; gene expression; genes; Populus tremula; enzyme activity; histochemistry; beta-glucuronidase
Abstract:
... Stem sections from poplar that were stably transformed with a eucalypt cinnamyl alcohol dehydrogenase promoter-beta-glucuronidase construct were prepared by using either a technique routinely used in herbaceous species or a technique designed to take into account the particular anatomy of woody plants. Although both preparation techniques confirmed the pattern of expression previously observed (C. ...

13. Cloning of the pumpkin ascorbate oxidase gene and analysis of a cis-acting region involved in induction by auxin

Author:
Kisu, Y.; Harada, Y.; Goto, M.; Esaka, M.
Source:
Plant & cell physiology 1997 v.38 no.5 pp. 631-637
ISSN:
0032-0781
Subject:
genetic code; beta-glucuronidase; ascorbate oxidase; DNA; Cucurbita; amino acid sequences; gene expression; reporter genes; enzyme activity; exons; promoter regions; introns
Abstract:
... A genomic clone encoding ascorbate oxidase was isolated from pumpkin (Cucurbita sp.). This gene is consisted of four exons and three introns. Analyses of the promoter fusion to beta-glucuronidase reporter gene by transient expression assay in pumpkin fruit tissues suggested the existence of a cis-acting region responsible for auxin regulation. ...
DOI:
10.1093/oxfordjournals.pcp.a029214
PubMed:
9210335

14. Colonization of tomato root by a non‐pathogenic strain of Fusarium oxysporum

Author:
CHANTAL OLIVAIN; CLAUDE ALABOUVETTE
Source:
new phytologist 1997 v.137 no.3 pp. 481-494
ISSN:
0028-646X
Subject:
cortex; cell walls; Fusarium oxysporum; defense mechanisms; hyphae; pathogenicity; reporter genes; roots; beta-glucuronidase; infection; Solanum lycopersicum var. lycopersicum
Abstract:
... A strain of non‐pathogenic Fusarium oxysporum Schlecht. emend, Snyd. & Hans. has been selected for its capacity to reduce the incidence of Fusarium wilt of tomato. Among the possible modes of action of this strain, competition with the pathogen for the colonization of the root surface and tissues has been proposed. In order to study the pattern of root colonization, young Lycopersicon etculentum M ...
DOI:
10.1046/j.1469-8137.1997.00855.x

15. Compromising early salicylic acid accumulation delays the hypersensitive response and increases viral dispersal during lesion establishment in TMV-infected tobacco

Author:
Mur, L.A.J.; Bi, Y.M.; Darby, R.M.; Firek, S.; Draper, J.
Source:
plant journal 1997 v.12 no.5 pp. 1113-1126
ISSN:
0960-7412
Subject:
Nicotiana tabacum; Tobacco mosaic virus; salicylic acid; defense mechanisms; gene expression; oxygenases; transgenic plants; necrosis; virulence; messenger RNA; beta-glucuronidase; reporter genes; pathogenesis; translocation (plant physiology); promoter regions
Abstract:
... To investigate the role of salicylic acid (SA) in the hypersensitive response (HR) its accumulation was compromised during different phases of lesion development by differential expression of a salicylate hydroxylase gene (SH-L). Constitutive suppression of SA accumulation was achieved by expression of a gene fusion between the CaMV35S promoter (35S) and SH-L. Using the H2O2-responsive AoPR1 promo ...
DOI:
10.1046/j.1365-313X.1997.12051113.x
PubMed:
9418052

16. Cutinase A of Botrytis cinerea is expressed, but not essential, during penetration of gerbera and tomato

Author:
Kan, J.A.L. van; Klooster, J.W. van't.; Wagemakers, C.A.M.; Dees, D.C.T.; Vlugt-Bergmans, C.J.B. van der.
Source:
Molecular plant-microbe interactions 1997 v.10 no.1 pp. 30-38
ISSN:
0894-0282
Subject:
genetic transformation; promoter regions; recombinant DNA; phenotype; tubulin; Botrytis cinerea; Gerbera; fungal diseases of plants; DNA probes; flowers; gene expression; pathogenicity; histochemistry; mutants; reporter genes; esterases; beta-glucuronidase; infection
Abstract:
... The plant pathogen Botrytis cinerea can infect undamaged plant tissue directly by penetration of the cuticle. This penetration has been suggested to be enzyme-mediated, and an important role for cutinase in the infection process has been proposed. In this study the expression of the cutinase encoding gene cutA of B. cinerea was analyzed using a cutA promoter-GUS reporter gene fusion. Transformants ...
DOI:
10.1094/MPMI.1997.10.1.30
PubMed:
9002270

17. Development and auxin-induced expression of the Arabidopsis prha homeobox gene

Author:
Plesch, G.; Stormann, K.; Torres, J.T.; Walden, R.; Somssich, I.E.
Source:
plant journal 1997 v.12 no.3 pp. 635-647
ISSN:
0960-7412
Subject:
Arabidopsis thaliana; transcription factors; plant tissues; plant development; messenger RNA; protoplasts; amino acid sequences; gene expression; plant vascular system; DNA-binding proteins; reporter genes; naphthaleneacetic acid; histochemistry; molecular weight; chromosome mapping; promoter regions; beta-glucuronidase
Abstract:
... A single-copy Arabidopsis homeobox gene, prha, which encodes a homeodomain protein with a molecular weight of 90 500 has been characterized. The position of the gene was mapped to the distal part of chromosome 4. Expression of the gene differs in various vegetative and floral plant tissues and is positively influenced by the phytohormone auxin. In Arabidopsis plants, a complex pattern of prha prom ...
DOI:
10.1046/j.1365-313X.1997.00635.x

18. Developmental regulation of pyrroline-5-carboxylate reductase gene expression in Arabidopsis

Author:
Hua, X.J.; Cotte, B. van de.; Montagu, M. van.; Verbruggen, N.
Source:
Plant physiology 1997 v.114 no.4 pp. 1215-1224
ISSN:
0032-0889
Subject:
guard cells; root tips; osmotic pressure; developmental stages; genes; shoot meristems; pollen; promoter regions; proline; gene expression; Arabidopsis thaliana; ovules; plant development; quantitative analysis; seeds; genetic code; transcription (genetics); enzyme activity; histochemistry; carboxylic acids; gene transfer; biosynthesis; transgenic plants; flowers; oxidoreductases; beta-glucuronidase
Abstract:
... At-P5R, a gene encoding the last enzyme of the proline (Pro) biosynthetic pathway in Arabidopsis thaliana, is developmentally regulated. To characterize the cis elements responsible for this developmental regulation, a series of 5' deletions of the At-P5R promoter were transcriptionally fused to a beta-glucuronidase (GUS)-coding region and transformed into Arabidopsis. The complete promoter of At- ...
PubMed:
9276946
PubMed Central:
PMC158414

19. Differential activation of two ACC oxidase gene promoters from melon during plant development and in response to pathogen attack

Author:
Lasserre, E.; Godard, F.; Bouquin, T.; Hernandez, J.A.; Pech, J.C.; Roby, D.; Balague, C.
Source:
MGG 1997 v.256 no.3 pp. 211-222
ISSN:
0026-8925
Subject:
ethylene; recombinant DNA; Ralstonia solanacearum; 2,4-D; promoter regions; gene expression; Nicotiana tabacum; mechanical damage; Pseudomonadaceae; oxygenases; infection; genetic recombination; copper sulfate; defense mechanisms; plant development; Cucumis melo; abiotic stress; histochemistry; transgenic plants; reporter genes; abscisic acid; beta-glucuronidase
DOI:
10.1007/s004380050563
PubMed:
9393445

20. Differential patterns of expression of the Arabidopsis PHYB, PHYD, and PHYE phytochrome genes

Author:
Goosey, L.; Palecanda, L.; Sharrock, R.A.
Source:
Plant physiology 1997 v.115 no.3 pp. 959-969
ISSN:
0032-0889
Subject:
root tips; seedling growth; developmental stages; promoter regions; photoreceptors; phenotype; gene expression; phytochrome; leaves; Arabidopsis thaliana; morphogenesis; amino acid sequences; enzyme activity; apoproteins; transgenic plants; flowers; mutants; reporter genes; genetic variation; plant morphology; beta-glucuronidase; light
Abstract:
... The Arabidopsis thaliana phyB, phyD, and phyE phytochrome apoproteins show higher amino acid sequence similarity to each other than to phyA or phyC, they are the most recently evolved members of this photoreceptor family, and they may interact in regulating photomorphogenesis. The expression patterns of translational fusions of the 5' upstream regions of the PHYB, PHYD, and PHYE genes to the beta- ...
PubMed:
9390432
PubMed Central:
PMC158559

21. Effect of Lentinus edodes on the growth of intestinal lactic acid bacteria

Author:
Eun-Ah Bae; Dong-Hyun Kim; Myung Joo Han
Source:
Archives of pharmacal research 1997 v.20 no.5 pp. 443-447
ISSN:
0253-6269
Subject:
beta-glucosidase; beta-glucuronidase; feces; gel chromatography; humans; intestinal microorganisms; lactic acid bacteria; trehalose; tryptophan 2,3-dioxygenase
Abstract:
... As the growth factor of lactic acid bacteria, LD (trehalose) was isolated from Lentinus edodes by using silica gel column chromatography. LD induced the growth of Bifidobacteria breve and Lactobacillus brevis, which were isolated from human feces. LD selectively induced the growth of lactic acid bacteria among total microflora. When total intestinal microflora were cultured in the medium containin ...
DOI:
10.1007/BF02973937

22. Electroporation as an alternative to microinjection of plasmid DNA into bollworm (Lepidoptera: Noctuidae) embryos

Author:
Hughes, K.J.; Narang, S.K.; Leopold, R.A.; Johnson, O.A.; DeVault, J.D.
Source:
Annals of the Entomological Society of America 1997 v.90 no.1 pp. 107-113
ISSN:
0013-8746
Subject:
Helicoverpa zea; gene transfer; genetic transformation; plasmid vectors; recombinant DNA; beta-glucuronidase; reporter genes; electroporation; direct DNA uptake; gene expression; mortality; biological development; embryo (animal)
DOI:
10.1093/aesa/90.1.107

23. Enzymuria as an index of renal damage in canine leishmaniasis

Author:
Palacio, J. Facultad de Veterinaria, Zaragoza, Spain.; Liste, F.; Gascon, M.
Source:
Veterinary record 1997 v.140 no.18 pp. 477-480
ISSN:
0042-4900
Subject:
dogs; leishmaniasis; kidney diseases; membrane alanyl aminopeptidase; gamma-glutamyltransferase; alkaline phosphatase; beta-N-acetylhexosaminidase; beta-glucuronidase; enzyme activity; urine; creatinine; blood; urea; nitrogen
DOI:
10.1136/vr.140.18.477
PubMed:
9160531

24. Expression and inheritance of foreign genes in transgenic peanut plants generated by Agrobacterium-mediated transformation

Author:
Cheng, M.; Jarret, R.L.; Li, Z.; Demski, J.W.
Source:
Plant cell reports 1997 v.16 no.8 pp. 541-544
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; transgenic plants; Agrobacterium radiobacter; gene expression; neomycin; phosphotransferases (phosphomutases); inheritance (genetics); micropropagation; Arachis hypogaea; enzyme activity; culture media; beta-glucuronidase; genetic markers
Abstract:
... To evaluate and characterize the stability of traits transferred via Agrobacterium transformation, foreign gene expression must be examined in sexually derived progeny. The objective of this study was to analyze three transgenic peanut plants, 1-10, 12-1, and 17-1, for the inheritance and expression of their foreign genes. Segregation ratios for the introduced genes in T2 plants gave either 100% o ...
DOI:
10.1007/s002990050275

25. Expression of ACC oxidase promoter-GUS fusions in tomato and Nicotiana plumbaginifolia regulated by developmental and environmental stimuli

Author:
Blume, B.; Grierson, D.
Source:
plant journal 1997 v.12 no.4 pp. 731-746
ISSN:
0960-7412
Subject:
ethylene; pericarp; seedlings; Nicotiana plumbaginifolia; promoter regions; Passalora fulva; Tobacco mosaic virus; jasmonic acid; gene expression; mechanical damage; petioles; multigene family; oxygenases; infection; messenger RNA; abscission; enzyme activity; histochemistry; Solanum lycopersicum var. lycopersicum; transgenic plants; Erysiphe; flowers; ripening; reporter genes; beta-glucuronidase
Abstract:
... The enzyme ACC oxidase, catalysing the last step in the biosynthesis of the plant hormone ethylene, is encoded by a small multigene family in tomato, comprising three members, LEACO1, LEACO2 and LEACO3. LEACO1 is the major gene expressed during ripening, leaf senescence, and wounding (Barry et al., 1996). To investigate the transcriptional regulation of ACC oxidase gene expression, chimeric fusion ...
DOI:
10.1046/j.1365-313X.1997.12040731.x
PubMed:
9375389

26. Factors affecting Agrobacterium tumefaciens-mediated gusA expression and opine synthesis in Gladiolus

Author:
Kamo, K.
Source:
Plant cell reports 1997 v.16 no.6 pp. 389-392
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; cultivars; alkaloids; biosynthesis; neoplasms; transgenic plants; Agrobacterium radiobacter; gene expression; Gladiolus; histochemistry; genes; etiolation; genetic variation; enzyme activity; roots; beta-glucuronidase; leaves
Abstract:
... Five tumorigenic strains of Agrobacterium tumefaciens were used to inoculate corms, cormels, plants grown in vitro, and seed-derived seedlings of several cultivars of Gladiolus. Tumors formed on 12% of the plant tissues inoculated, and 1% of these tumors synthesized either octopine or nopaline. A. tumefaciens-mediated beta-glucuronidase (GUS) expression showed 0.5% and 3.5% GUS expression for plan ...
Handle:
10113/42284
DOI:
10.1007/BF01146779

27. Factors affecting Agrobacterium-mediated transformation in Citrus and production of sour orange (Citrus aurantium L.) plants expressing the coat protein gene of citrus tristeza virus

Author:
Gutierrez-E, M.A.; Luth, D.; Moore, G.A.
Source:
Plant cell reports 1997 v.16 no.11 pp. 745-753
ISSN:
0721-7714
Subject:
developmental stages; benzyladenine; genes; shoots; dose response; Citrus tristeza virus; Agrobacterium radiobacter; explants; gene expression; coat proteins; Citrus aurantium; Citrus aurantiifolia; enzyme activity; culture media; histochemistry; genetic transformation; gene transfer; kanamycin; transgenic plants; internodes; beta-glucuronidase
Abstract:
... Factors influencing transformation frequencies using the Agrobacterium-mediated protocol developed for Citrus seedling internodal stem segments in this laboratory were evaluated, with particular emphasis on decreasing the numbers of "escape" shoots produced. Although the use of a wild-type "shooty" Agrobacterium strain allowed relatively high frequencies of beta-glucuronidase positive (GUS+) shoot ...
DOI:
10.1007/s002990050313

28. A G-box-binding protein from soybean binds to the E1 auxin-response element in the soybean GH3 promoter and contains a proline-rich repression domain

Author:
Liu, Z.B.; Hagen, G.; Guilfoyle, T.J.
Source:
Plant physiology 1997 v.115 no.2 pp. 397-407
ISSN:
0032-0889
Subject:
protoplasts; gene transfer; Glycine max; leucine; genetic code; transgenic plants; Daucus carota; proline; luciferase; binding proteins; gene expression; plasmids; auxins; Nicotiana tabacum; transcription (genetics); reporter genes; enzyme activity; beta-glucuronidase
Abstract:
... The E1 promoter fragment (-249 to -203) is one of three auxin-response elements (AuxREs) in the soybean (Glycine max L.) GH3 promoter (Z.-B. Liu, T. Ulmasov, X. Shi, G. Hagen, T.J. Guilfoyle [1994] Plant Cell 6: 645-657). Results presented here further characterize and delimit the AuxRE within the E1 fragment. The E1 fragment functioned as an AuxRE in transgenic tobacco (Nicotiana tabacum L.) plan ...

29. Gene fusions of signal sequences with a modified beta-glucuronidase gene results in retention of the beta-glucuronidase protein in the secretory pathway/plasma membrane

Author:
Yan, X.H.; Gonzales, R.A.; Wagner, G.J.
Source:
Plant physiology 1997 v.115 no.3 pp. 915-924
ISSN:
0032-0889
Subject:
Phaseolus vulgaris; Manduca sexta; Nicotiana tabacum; leaves; callus; endoplasmic reticulum; sucrose; biochemical pathways; protein composition; beta-glucuronidase; enzyme activity; reporter genes; transgenic plants; gene expression; cytosol; gene transfer; genetic vectors; genetic transformation; plasma membrane; translocation (plant physiology); plant secretions and exudates
Abstract:
... Signal sequences and endoplasmic reticulum (ER)retention signals are known to play central roles in targeting and translocation in the secretory pathway, but molecular aspects about their involvement are poorly understood. We tested the effectiveness of deduced signal sequences from various genes (hydroxyproline-rich glycoprotein [HRGP] from Phaseolus vulgaris; Serpin from Manduca sexta) to direct ...
PubMed:
9390428
PubMed Central:
PMC158555

30. Gene targeting and instability of Agrobacterium T-DNA loci in the plant genome

Author:
Risseeuw, E.; Franke-van Dijk, M.E.I.; Hooykaas, P.J.J.
Source:
plant journal 1997 v.11 no.4 pp. 717-728
ISSN:
0960-7412
Subject:
genome; flucytosine; micropropagation; Nicotiana plumbaginifolia; callus; drug resistance; DNA; Agrobacterium radiobacter; Nicotiana tabacum; leaves; plant development; enzyme activity; protoplasts; gene transfer; genetic transformation; cytosine deaminase; kanamycin; loci; transgenic plants; genetic stability; homologous recombination; nucleotide sequences; beta-glucuronidase
Abstract:
... To develop a model system for studies of homologous recombination in plants, transgenic Nicotiana tabacum and Nicotiana plumbaginifolia lines were generated harbouring a single target T-DNA containing the negative selective codA gene encoding cytosine deaminase (CD) and the beta-glucuronidase (GUS) gene. Subsequently, the target lines were transformed with a replacement-type T-DNA vector in which ...
DOI:
10.1046/j.1365-313X.1997.11040717.x
PubMed:
9161032

31. Genetic and developmental control of nuclear accumulation of COP1, a repressor of photomorphogenesis in Arabidopsis

Author:
Von Arnim, A.G.; Osterlund, M.T.; Kwok, S.F.; Deng, X.W.
Source:
Plant physiology 1997 v.114 no.3 pp. 779-788
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; seedlings; cell growth; cytokinins; morphogenesis; light; reporter genes; loci; beta-glucuronidase; enzyme activity; gene expression; hypocotyls; mutants; brassinosteroids; genetic variation; mutation
Abstract:
... Using a beta-glucuronidase (GUS) reporter-COP1 fusion transgene, it was shown previously that Arabidopsis COP1 acts within the nucleus as a repressor of seedling photomorphogenic development and that light inactivation of COP1 was accompanied by a reduction of COP1 nuclear abundance (A.G. von Arnim, X.-W. Deng [1994] Cell 79: 1035-1045). Here we report that the GUS-COP1 fusion transgene can comple ...
PubMed:
9232869
PubMed Central:
PMC158364

32. Genetic transformation of the C3-C4 intermediate plant, Flaveria pubescens (Asteraceae)

Author:
Chu, C.C.; Qu, N.; Bassuner, B.; Bauwe, H.
Source:
Plant cell reports 1997 v.16 no.10 pp. 715-718
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; promoter regions; dose response; Agrobacterium radiobacter; benzyladenine; reporter genes; naphthaleneacetic acid; Flaveria pringlei; photosynthesis; culture media; beta-glucuronidase
Abstract:
... The dicot genus Flaveria (Asteraceae), besides species with C3 or C4 photosynthesis, contains taxa with a broad range of different states of transition between the two major photosynthetic types. We have developed a reproducible and efficient Agrobacterium-mediated method for the stable genetic transformation of the C3-C4 intermediate species F. pubescens. Fusion constructs of the reporter gene be ...
DOI:
10.1007/s002990050308

33. Genetic transformation of the actinorhizal tree Allocasuarina verticillata by Agrobacterium tumefaciens

Author:
Franche, C.; Diouf, D.; Le, Q.V.; Bogusz, D.; N'Diaye, A.; Gherbi, H.; Gobe, C.; Duhoux, E.
Source:
plant journal 1997 v.11 no.4 pp. 897-904
ISSN:
0960-7412
Subject:
genetic transformation; callus; nitrogen fixation; kanamycin; transgenic plants; embryo (plant); Agrobacterium radiobacter; root nodules; gene expression; Allocasuarina; reporter genes; Frankia; phosphotransferases (kinases); histochemistry; beta-glucuronidase; genetic markers; drug resistance
Abstract:
... We have developed an efficient transformation system for the tropical actinorhizal tree Allocasuarina verticillata using Agrobacterium tumefaciens mediated gene transfer. Mature zygotic embryos were inoculated with the disarmed strain C58C1 carrying, in the binary vector BIN19, the nptII gene, providing kanamycin resistance as a selectable marker, and the reporter gene beta-glucuronidase containin ...
DOI:
10.1046/j.1365-313X.1997.11040897.x

34. Independent modulation of Arabidopsis thaliana polyubiquitin mRNAs in different organs and in response to environmental changes

Author:
Sun, C.W.; Callis, J.
Source:
plant journal 1997 v.11 no.5 pp. 1017-1027
ISSN:
0960-7412
Subject:
Arabidopsis thaliana; plant tissues; promoter regions; messenger RNA; stems; nitrogen; nutrient deficiencies; flowers; heat stress; transcription (genetics); multigene family; reporter genes; etiolation; ubiquitin; light; roots; beta-glucuronidase; leaves; shoots
Abstract:
... The highly conserved protein ubiquitin is encoded by five polyubiquitin genes in Arabidopsis thaliana ecotype Columbia that have been divided into two subtypes, the UBQ3/UBQ4 subtype and the UBQ10/UBQ11/UBQ14 subtype. Northern analysis using gene-specific oligonucleotides as hybridization probes and enzyme activity measurements from transgenic plants expressing beta-glucuronidase (GUS) under the c ...
DOI:
10.1046/j.1365-313X.1997.11051017.x
PubMed:
9193073

35. Inducers of aflatoxin biosynthesis from colonized maize kernels are generated by an amylase activity from Aspergillus flavus

Author:
Woloshuk, C.P.; Cavaletto, J.R.; Cleveland, T.E.
Source:
Phytopathology 1997 v.87 no.2 pp. 164-169
ISSN:
0031-949X
Subject:
Zea mays; biosynthesis; seeds; quantitative analysis; nonreducing sugars; glucose; maltotriose; starch; aflatoxins; metabolites; plant pathogenic fungi; maltose; reporter genes; glycolysis; culture filtrates; enzyme activity; alpha-amylase; beta-glucuronidase; Aspergillus flavus
Abstract:
... Aflatoxin biosynthesis was induced by compounds in filtrates (EF) obtained from cultures consisting of ground maize kernels colonized by Aspergillus flavus. The inducing activity increased to a maximum at 4 days of incubation and then decreased. Amylase activity was detected in the EF, suggesting that the inducers are products of starch degradation (glucose, maltose, and maltotriose). Analysis of ...
Handle:
10113/23536
DOI:
10.1094/PHYTO.1997.87.2.164
PubMed:
18945137

36. Integration of T-DNA binary vector 'backbone' sequences into the tobacco genome: evidence for multiple complex patterns of integration

Author:
Kononov, M.E.; Bassuner, B.; Gelvin, S.B.
Source:
plant journal 1997 v.11 no.5 pp. 945-957
ISSN:
0960-7412
Subject:
genetic transformation; transgenic plants; Agrobacterium radiobacter; plasmid vectors; gene expression; Nicotiana tabacum; reporter genes; beta-glucuronidase
Abstract:
... During the process of crown gall tumorigenesis, Agrobacterium tumefaciens transfers part of the tumor-inducing (Ti) plasmid, the T-DNA, to a plant cell where it eventually becomes stably integrated into the plant genome. Directly repeated DNA sequences, called T-DNA borders, define the left and the right ends of the T-DNA. The T-DNA can be physically separated from the remainder of the Ti-plasmid, ...
DOI:
10.1046/j.1365-313X.1997.11050945.x
PubMed:
9193068

37. Intracellular distribution and identification of the nuclear localization signals of two plant heat-stress transcription factors

Author:
Lyck, R.; Harmening, U.; Hohfeld, I.; Treater, E.; Scharf, K.D.; Nover, L.
Source:
Planta 1997 v.202 no.1 pp. 117-125
ISSN:
0032-0935
Subject:
protoplasts; messenger RNA; heat stress; gene expression; amino acids; chromosome mapping; DNA-binding proteins; nucleotide sequences; cytoplasm; DNA-binding domains; Nicotiana plumbaginifolia; signal peptide; immunocytochemistry; beta-glucuronidase; Solanum lycopersicum var. lycopersicum
Abstract:
... Similar to heat-stress transcription factors (HSFs) from non-plant sources, HSFA1 and HSFA2 from tomato (Lycopersicon esculentum Mill) contain two conserved clusters of basic amino acid residues (K/R1 and K/R2) which might serve as nuclear localization signal (NLS) motifs. Mutation of either one of them and functional testing of the corresponding proteins in a transient expression assay using toba ...
DOI:
10.1007/s004250050110
PubMed:
9177056

38. Introns act post-transcriptionally to increase expression of the Arabidopsis thaliana tryptophan pathway gene PAT1

Author:
Rose, A.B.; Last, R.L.
Source:
plant journal 1997 v.11 no.3 pp. 455-464
ISSN:
0960-7412
Subject:
recombinant DNA; Arabidopsis thaliana; gene expression; introns; biochemical pathways; tryptophan; transferases; beta-glucuronidase; enzyme activity; plasmids; reporter genes; messenger RNA; transgenic plants; plasmid vectors; promoter regions; transcription (genetics); histochemistry
Abstract:
... The expression of the Arabidopsis thaliana PAT1 gene, which encodes the tryptophan biosynthetic enzyme phosphoribosylanthranilate transferase, was investigated using translational fusions of the PAT1 promoter to the GUS reporter gene. Independent stably transformed A. thaliana lines containing a single copy of a fusion that includes the entire plastid transit peptide and the first two introns of P ...
DOI:
10.1046/j.1365-313X.1997.11030455.x
PubMed:
9107035

39. Larix laricina (tamarack): somatic embryogenesis and genetic transformation

Author:
Klimaszewska, K.; Devantier, Y.; Lachance, D.; Lelu, M.A.; Charest, P.J.
Source:
Canadian journal of forest research = 1997 v.27 no.4 pp. 538-550
ISSN:
0045-5067
Subject:
Larix laricina; somatic embryogenesis; genetic transformation; osmotic pressure; abscisic acid; sucrose; polyethylene glycol; germination; biolistics; genetic vectors; kanamycin; hygromycin B; methotrexate; beta-glucuronidase; transgenic plants; roots; hypocotyls; cotyledons
Abstract:
... Excised, immature zygotic embryos of Larix laricina (Du Roi) K. Koch (tamarack) gave rise to embryogenic cultures on modified Murashige and Skoog (MSG) medium supplemented with growth regulators. Three lines of embryonal masses were maintained for 1 year by biweekly subcultures prior to the maturation experiments. All of these lines showed the ability to produce mature somatic embryos. Both elevat ...
DOI:
10.1139/x96-208

40. Light modulates the spatial patterns of 3-hydroxy-3-methylglutaryl coenzyme A reductase gene expression in Arabidopsis thaliana

Author:
Learned, R.M.; Connolly, E.L.
Source:
plant journal 1997 v.11 no.3 pp. 499-511
ISSN:
0960-7412
Subject:
Arabidopsis thaliana; promoter regions; messenger RNA; recombinant DNA; seedlings; gene expression; histochemistry; reporter genes; hydroxymethylglutaryl-CoA reductases; roots; beta-glucuronidase; light
Abstract:
... Although the coordinated biosynthesis of isoprenoid compounds is thought to be essential to the normal processes of plant growth and development, the mechanisms that regulate the mevalonate pathway in plants are not well understood. As the first committed step in the pathway, the conversion of 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) to mevalonic acid by HMG CoA reductase and the regulation ...
DOI:
10.1046/j.1365-313X.1997.11030499.x
PubMed:
9107038

41. Light-induced transcriptional repression of the pea AS1 gene: identification of cis-elements and transfactors

Author:
Ngai, N.; Tsai, F.Y.; Coruzzi, G.
Source:
plant journal 1997 v.12 no.5 pp. 1021-1034
ISSN:
0960-7412
Subject:
transcription factors; transgenic plants; plasmid vectors; transcription (genetics); chromosome mapping; Nicotiana tabacum; DNA-binding proteins; reporter genes; Pisum sativum; aspartate-ammonia ligase; promoter regions; beta-glucuronidase; light
Abstract:
... Here, we examine the cis-elements and trans-factors affecting the expression of asparagine synthetase (AS) genes whose transcription is negatively regulated by light. The promoters for the AS1 and AS2 genes of pea were isolated, sequenced, and functionally dissected for their ability to confer regulated expression to the GUS reporter gene in transgenic tobacco. Histochemical analysis of transgenic ...
DOI:
10.1046/j.1365-313X.1997.12051021.x

42. Light-regulated expression of the pea plastocyanin gene is mediated by elements within the transcribed region of the gene

Author:
Helliwell, C.A.; Webster, C.I.; Gray, J.C.
Source:
plant journal 1997 v.12 no.3 pp. 499-506
ISSN:
0960-7412
Subject:
messenger RNA; ribosomal RNA; transgenic plants; gene expression; Nicotiana tabacum; reporter genes; Pisum sativum; promoter regions; beta-glucuronidase; light
Abstract:
... Expression of the pea plastocyanin gene (PetE) is regulated by light in both pea and transgenic tobacco plants. However, the PetE promoter with the 5' untranslated leader region does not direct light-regulated expression of the GUS reporter gene in transgenic tobacco. This suggested that sequences downstream of the translation start of the PetE gene are required for light-regulated expression. To ...
DOI:
10.1046/j.1365-313X.1997.00499.x
PubMed:
9351238

43. Light-responsive elements of the tobacco PSI-D gene are located both upstream and within the transcribed region

Author:
Yamamoto, Y.Y.; Kondo, Y.; Kato, A.; Tsuji, H.; Obokata, J.
Source:
plant journal 1997 v.12 no.2 pp. 255-265
ISSN:
0960-7412
Subject:
messenger RNA; Nicotiana sylvestris; transgenic plants; plasmid vectors; seedlings; binding proteins; gene expression; transcription (genetics); reporter genes; photosystem I; start codon; promoter regions; beta-glucuronidase; leaves
Abstract:
... psaDb is a nuclear gene encoding the ferredoxin-binding subunit of photosystem I in Nicotiana sylvestris. The organization of the light-responsive cis-elements of psaDb was studied using transgenic tobacco plants. Three types of psaDb chimeric constructs were created: a 5' upstream fragment of psaDb transcriptionally fused with the beta-glucuronidase (GUS) gene, and a series of its 5' deletion der ...
DOI:
10.1046/j.1365-313X.1997.12020255.x
PubMed:
9301080

44. Localization of expression of KNAT3, a class 2 knotted1-like gene

Author:
Serikawa, K.A.; Martinez-Laborda, A.; Kim, H.S.; Zambryski, P.C.
Source:
plant journal 1997 v.11 no.4 pp. 853-861
ISSN:
0960-7412
Subject:
Arabidopsis thaliana; far-red light; plant tissues; plant development; transcription factors; recombinant DNA; promoter regions; morphogenesis; homeotic genes; photoperiod; gene expression; red light; reporter genes; nucleic acid hybridization; histochemistry; beta-glucuronidase
Abstract:
... KNAT3 is a class 2 kn1-like gene in Arabidopsis thaliana. The RNA expression patterns of KNAT3 were characterized through the use of promoter-GUS fusion analysis and in situ hybridization. KNAT3 is expressed in several tissues and at several times during development. There are three main expression patterns: (1) during early organ development in young leaves, buds and pedicels; (2) at and near the ...
DOI:
10.1046/j.1365-313X.1997.11040853.x
PubMed:
9161040

45. Modulation of carbon and nitrogen metabolism, and of nitrate reductase, in untransformed and transformed Nicotiana plumbaginifolia during CO2 enrichment of plants grown in pots and in hydroponic culture

Author:
Ferrario-Mery, S.; Thibaud, M.C.; Betsche, T.; Valadier, M.H.; Foyer, C.H.
Source:
Planta 1997 v.202 no.4 pp. 510-521
ISSN:
0032-0935
Subject:
Nicotiana plumbaginifolia; transgenic plants; nitrate reductase; beta-glucuronidase; carbon nitrogen ratio; hydroponics; shoots; dry matter accumulation; starch; sucrose; leaves; nitrates; glutamine; enzyme activity; messenger RNA; metabolism; nitrogen; carbon dioxide; container-grown plants; chemical constituents of plants; histochemistry
Abstract:
... Transformed plants of Nicotiana plumbaginifolia Viv. constitutively expressing nitrate reductase (35S-NR) or beta-glucuronidase (35S-GUS) and untransformed controls were grown for two weeks in a CO2-enriched atmosphere. Whereas CO2 enrichment (1000 microliter.l-1) resulted in an increase in the carbon (C) to nitrogen (N) ratio of both the tobacco lines grown in pots with vermiculite, the C/N ratio ...
DOI:
10.1007/s004250050156

46. Optimization of parameters for particle bombardment of embryogenic suspension cultures of cassava (Manihot esculenta Crantz) using computer image analysis

Author:
Schopke, C.; Taylor, N.J.; Carcamo, R.; Beachy, R.N.; Fauquet, C.
Source:
Plant cell reports 1997 v.16 no.8 pp. 526-530
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; image analysis; beta-glucuronidase; computer analysis; plasmids; gene expression; genes; leaves; micropropagation; enzyme activity; cell suspension culture; Manihot esculenta
Abstract:
... Tissue derived from embryogenic suspension cultures of cassava was bombarded with microparticles coated with a plasmid containing the uidA gene, which codes for beta-glucuronidase (GUS). After 3 days, the effect of different bombardment parameters was evaluated by comparing the numbers of blue spots that resulted from histological GUS assays. Counting of blue spots was performed using a system com ...
DOI:
10.1007/s002990050272

47. Potential of biolistic transformation of barley microspores based on viability and transient beta-glucuronidase activity

Author:
Yao, Q.A.; Kasha, K.J.
Source:
Genome = 1997 v.40 no.5 pp. 639-643
ISSN:
0831-2796
Subject:
Hordeum vulgare; genetic transformation; biolistics; cells; pollen; reporter genes; beta-glucuronidase; enzyme activity; viability
Abstract:
... Microspores could be an excellent target for plant transformation, owing to their haploid nature, the availability of a large population of fairly synchronous single cells, and their potential to regenerate into plants through embryogenesis. Therefore, the potential for microspore transformation by biolistic procedures was examined cytologically, based on the viability and beta-glucuronidase (GUS) ...
DOI:
10.1139/g97-084

48. Promoter analysis and expression of a phospholipase D gene from castor bean

Author:
Xu, L.; Zheng, S.; Zheng, L.; Wang, X.
Source:
Plant physiology 1997 v.115 no.2 pp. 387-395
ISSN:
0032-0889
Subject:
cell growth; corolla; seedlings; genes; cell differentiation; pollen; promoter regions; calyx; phosphoric diester hydrolases; gynoecium; hypocotyls; meristems; Ricinus communis; gene expression; Nicotiana tabacum; leaves; messenger RNA; buds; histology; enzyme activity; vascular bundles; gene transfer; transgenic plants; sexual reproduction; beta-glucuronidase
Abstract:
... The expression of a castor bean (Ricinus communis L.) phospholipase D (PLD; EC 3.1.4.4) gene has been studied by examining its promoter activity in transgenic tobacco (Nicotiana tabacum) carrying a PLD promoter-glucuronidase transgene and by monitoring the levels of PLD mRNA in castor bean. Sequence and the 5' truncation analyses revealed that the 5' flanking region from nucleotide -1200 to -730 i ...
PubMed:
9342861
PubMed Central:
PMC158496

49. Protein dephosphorylation mediates salicylic acid-induced expression of PR-1 genes in tobacco

Author:
Conrath, U.; Silva, H.; Klessig, D.F.
Source:
plant journal 1997 v.11 no.4 pp. 747-757
ISSN:
0960-7412
Subject:
Nicotiana tabacum; salicylic acid; gene expression; catalase; ascorbate oxidase; hydrogen peroxide; enzyme inhibitors; serine; threonine; transgenic plants; protein transport; actin; phenylalanine ammonia-lyase; beta-glucuronidase; messenger RNA; phosphoprotein phosphatase; pathogenesis-related proteins; phosphoproteins
Abstract:
... Several lines of evidence suggest that salicylic acid (SA) is an endogenous signal for the activation of several plant defense responses, including the expression of genes encoding pathogenesis-related (PR) proteins such as the acidic PR-1 proteins. During recent years, studies have suggested that interaction of SA with catalase and ascorbate peroxidase leads to two signals in tobacco -- elevated ...
DOI:
10.1046/j.1365-313X.1997.11040747.x

50. Regeneration of transgenic vegetable brassicas (Brassica oleracea and B. campestris) via Ri-mediated transformation

Author:
Christey, M.C.; Sinclair, B.K.; Braun, R.H.; Wyke, L.
Source:
Plant cell reports 1997 v.16 no.9 pp. 587-593
ISSN:
0721-7714
Subject:
Rhizobium rhizogenes; benzyladenine; neomycin; Brassica rapa subsp. oleifera; roots; shoots; phenotype; gene expression; genotype; naphthaleneacetic acid; cultivars; phosphotransferases (phosphomutases); enzyme activity; Brassica oleracea; culture media; genetic transformation; gene transfer; transgenic plants; cultured cells; genetic variation; genetic vectors; beta-glucuronidase
Abstract:
... A procedure for the production of fertile transgenic brassicas via Ri-mediated transformation is reported in this paper. Transgenic hairy root lines were selected for 12 vegetable brassica cultivars and lines representing six varieties: broccoli, Brussels sprouts, cabbage, cauliflower, rapid-cycling (all Brassica oleracea) and Chinese cabbage (B. campestris). Leaf explants or petioles of intact co ...
DOI:
10.1007/BF01275497