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1. Conformational Behavior of Chitosan in the Acetate Salt: An X-Ray Study

Author:
Atsushi Yamamoto; Junpei Kawada; Toshifumi Yui; Kozo Ogawa
Source:
Bioscience, biotechnology, and biochemistry 1997 v.61 no.7 pp. 1230-1232
ISSN:
1347-6947
Subject:
X-radiation; acetates; acetic acid; biotechnology; chitin; chitosan; crabs; solvents
Abstract:
... A well-defined X-ray fiber pattern of chitosan acetate was obtained by immersing a tendon chitosan, prepared from a crab tendon chitin by a solid-state N-deacetylation, in an aqueous acetic acid-isopropanol solution at 110°C. This pattern was very similar to that of chitosan salts with some inorganic acids, such as HF, HCl, and H₂SO₄, in which chitosan chains form an 8/5 helix, indicating that chi ...
DOI:
10.1271/bbb.61.1230

2. Differential induction of lipoxygenase isoforms in wheat upon treatment with rust fungus elicitor, chitin oligosaccharides, chitosan, and methyl jasmonate

Author:
Bohland, C.; Balkenhohl, T.; Loers, G.; Feussner, I.; Grambow, H.J.
Source:
Plant physiology 1997 v.114 no.2 pp. 679-685
ISSN:
0032-0889
Subject:
protein synthesis; dose response; chitosan; seedlings; jasmonic acid; linoleate 13S-lipoxygenase; Puccinia graminis; Triticum aestivum; leaves; plant pathogenic fungi; oligosaccharides; chitin; enzyme activity; isozymes; protein composition
Abstract:
... A glycopeptide elicitor prepared from germ tubes of the rust fungus Puccinia graminis Pers. f. sp. tritici Erikss. & Henn (Pgt),as well as chitin oligosaccharides, chitosan, and methyl jasmonate (MJ) stimulated lipoxygenase (LOX) activity (E.C. 1.13.11.12) in wheat (Triticum aestivum) leaves. Immunoblot analysis using anti-LOX antibodies revealed the induction of 92- and 103-kD LOX species after P ...

3. Elicitor Actions of N-Acetylchitooligosaccharides and Laminarioligosaccharides for Chitinase and l-Phenylalanine Ammonia-lyase Induction in Rice Suspension Culture

Author:
Hiroshi Inui; Yasuhiro Yamaguchi; Shigehiro Hirano
Source:
Bioscience, biotechnology, and biochemistry 1997 v.61 no.6 pp. 975-978
ISSN:
1347-6947
Subject:
biotechnology; chitin; chitinase; chitosan; dose response; elicitors; oligosaccharides; phenylalanine; rice
Abstract:
... When a series of chitin oligosaccharides was added into a rice suspension culture, N-acetylchito- hexaose, N-acetylchitopentaose, and N-acetylchitotetraose caused an increase in extracellular chitinase activity, mainly due to induction of a class III chitinase. In the case of N-acetylchitohexaose, a substantial increase in the chitinase activity was observed at a concentration higher than 0.01 μg/ ...
DOI:
10.1271/bbb.61.975

4. Purification and Characterization of the Chitinase (ChiA) from Enterobacter sp. G-l

Author:
Jae Kweon Park; Kenji Morita; Ikuo Fukumoto; Yukikazu Yamasaki; Tsuyoshi Nakagawa; Makoto Kawamukai; Hideyuki Matsuda
Source:
Bioscience, biotechnology, and biochemistry 1997 v.61 no.4 pp. 684-689
ISSN:
1347-6947
Subject:
Enterobacter; Salvia hispanica; ammonium sulfate; biotechnology; calcium; carboxymethylcellulose; chitin; chitinase; chitosan; culture filtrates; fractionation; gel chromatography; isoelectric point; molecular weight; pH; polyacrylamide gel electrophoresis; temperature
Abstract:
... Entevobacter sp. G-1 which produces chitinolytic and chitosanolytic enzymes, was previously isolated in our laboratory. One major chitinase, designated ChiA, was purified 42.9-fold from a culture filtrate of Entevobacter sp. G-L To purify the chitinase, ammonium sulfate fractionation, DEAE-Sephadex A-50 column chromatography, and gel filtration on Sephadex G-100 column chromatography were used. Th ...
DOI:
10.1271/bbb.61.684