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You searched for: Publication Year 1997 Remove constraint Publication Year: 1997 Subject term Brassica napus Remove constraint Subject term: Brassica napus Subject term Nicotiana tabacum Remove constraint Subject term: Nicotiana tabacum
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1. Importance of the chiral centers of jasmonic acid in the responses of plants. Activities and antagonism between natural and synthetic analogs

Author:
Holbrook, L.; Tung, P.; Ward, K.; Reid, D.M.; Abrams, S.; Lamb, N.; Quail, J.W.; Moloney, M.M.
Source:
Plant physiology 1997 v.114 no.2 pp. 419-428
ISSN:
0032-0889
Subject:
Helianthus annuus; Brassica napus; measurement; chemical degradation; dose response; jasmonic acid; gene expression; Triticum aestivum; Nicotiana tabacum; esters; stereochemistry; enzyme activity; chemical structure; seed germination; transgenic plants; cotyledons; proteinase inhibitors; reporter genes; chemical constituents of plants; chlorophyll; beta-glucuronidase
Abstract:
... The importance of the two chiral centers at C-3 and C-7 in the molecular structure of jasmonic acid in plant responses was investigated. We separated methyl jasmonate (MeJA) into (3R)- and (3S)-isomers with a fixed stereochemistry at C-3, but epimerization at C-7 is possible. The four isomers of the nonepimerizable analog 7-methyl MeJA were synthesized. These six esters and their corresponding aci ...

2. Proline- and hydroxyproline-rich gene products in the sexual tissues of flowers

Author:
Sommer-Knudsen, J.; Clarke, A.E.; Bacic, A.
Source:
Sexual plant reproduction 1997 v.10 no.5 pp. 253-260
ISSN:
0934-0882
Subject:
Helianthus annuus; Oryza sativa; Solanum tuberosum; hydroxyproline; genes; Brassica napus; extensin; proline; clones; gene expression; Nicotiana tabacum; protein composition; Arabidopsis thaliana; Zea mays; genetic code; Nicotiana alata; flowers; sexual reproduction; binding sites; Antirrhinum
DOI:
10.1007/s004970050095

3. Screening of transgenic plants by multiplex PCR

Author:
Marie Mannerlöf; Paul Tenning
Source:
Plant molecular biology reporter 1997 v.15 no.1 pp. 38-45
ISSN:
0735-9640
Subject:
genetic transformation; Zea mays; Helianthus annuus; Petunia hybrida; Capsicum annuum; Cucumis melo; Lactuca sativa; shoots; Solanum tuberosum; transgenic plants; polymerase chain reaction; rapid methods; Cucurbita pepo; Nicotiana tabacum; Brassica napus; Beta vulgaris; Solanum lycopersicum var. lycopersicum
Abstract:
... A protocol is described, for the rapid screening of a large number of putative transgenic shoots. Genomic DNA is isolated and screened by PCR. To validate the purity of the DNA, PCR amplification is done with primers homologous to an endogenous gene. Multiplex PCR is used to screen for the transgenic shoots with two sets of primers, one set against the endogenous gene (internal control) and the ot ...
DOI:
10.1007/BF02772111

4. cDNA clones encoding 1,3-beta-glucanase and a fimbrin-like cytoskeletal protein are induced by Al toxicity in wheat roots

Author:
Cruz-Ortega, R.; Cushman, J.C.; Ownby, J.D.
Source:
Plant physiology 1997 v.114 no.4 pp. 1453-1460
ISSN:
0032-0889
Subject:
Triticum aestivum; Brassica napus; Arabidopsis thaliana; Hordeum vulgare; Nicotiana tabacum; Pisum sativum; roots; aluminum; phytotoxicity; protein synthesis; actin; molecular weight; isoelectric point; beta-glucanase; cytoskeleton; protein composition; genetic code; complementary DNA; genes; gene expression; clones; cultivars; genetic variation; amino acid sequences; messenger RNA; stress response
Abstract:
... A cDNA library made from mRNA of Al-treated roots of an Al-sensitive wheat (Triticum aestivum cv Victory) cultivar was screened with a degenerate oligonucleotide probe derived from the partial amino acid sequence of the Al-induced protein TAl-18. Of seven clones that initially hybridized with the probe, one encoded a novel 1,3-beta-glucanase having a calculated molecular weight of 46.3 and an isoe ...
PubMed:
9276954
PubMed Central:
PMC158438