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1997
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dose response
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beta-glucuronidase
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- Author:
- Gutierrez-E, M.A.; Luth, D.; Moore, G.A.
- Source:
- Plant cell reports 1997 v.16 no.11 pp. 745-753
- ISSN:
- 0721-7714
- Subject:
- developmental stages; benzyladenine; genes; shoots; dose response; Citrus tristeza virus; Agrobacterium radiobacter; explants; gene expression; coat proteins; Citrus aurantium; Citrus aurantiifolia; enzyme activity; culture media; histochemistry; genetic transformation; gene transfer; kanamycin; transgenic plants; internodes; beta-glucuronidase
- Abstract:
- ... Factors influencing transformation frequencies using the Agrobacterium-mediated protocol developed for Citrus seedling internodal stem segments in this laboratory were evaluated, with particular emphasis on decreasing the numbers of "escape" shoots produced. Although the use of a wild-type "shooty" Agrobacterium strain allowed relatively high frequencies of beta-glucuronidase positive (GUS+) shoot ...
- DOI:
- 10.1007/s002990050313
- https://doi.org/10.1007/s002990050313
- Author:
- Chu, C.C.; Qu, N.; Bassuner, B.; Bauwe, H.
- Source:
- Plant cell reports 1997 v.16 no.10 pp. 715-718
- ISSN:
- 0721-7714
- Subject:
- genetic transformation; gene transfer; promoter regions; dose response; Agrobacterium radiobacter; benzyladenine; reporter genes; naphthaleneacetic acid; Flaveria pringlei; photosynthesis; culture media; beta-glucuronidase
- Abstract:
- ... The dicot genus Flaveria (Asteraceae), besides species with C3 or C4 photosynthesis, contains taxa with a broad range of different states of transition between the two major photosynthetic types. We have developed a reproducible and efficient Agrobacterium-mediated method for the stable genetic transformation of the C3-C4 intermediate species F. pubescens. Fusion constructs of the reporter gene be ...
- DOI:
- 10.1007/s002990050308
- https://doi.org/10.1007/s002990050308
- Author:
- Stefanov, I.; Frank, J.; Gedamu, L.; Misra, S.
- Source:
- Plant cell reports 1997 v.16 no.5 pp. 291-294
- ISSN:
- 0721-7714
- Subject:
- Nicotiana tabacum; cadmium; dose response; phytotoxicity; plant breeding; chromosome mapping; gene transfer; transgenic plants; chimerism; reporter genes; gene expression; seedlings; callus; beta-glucuronidase; enzyme activity; measurement; glucuronic acid; transcription (genetics); promoter regions
- Abstract:
- ... Transgenic tobacco plants and calli bearing the bacterial uidA A gene under the transcriptional control of rbcS, mas and CaMV35S promoter(s) were exposed to different concentrations of cadmium. The transcriptional activity of the promoters was monitored using p-nitrophenyl beta-D-glucuronide as a substrate for the beta-glucuronidase (uidA) reporter enzyme. The rbcS promoter was repressed by high c ...
- DOI:
- 10.1007/BF01088283
- https://doi.org/10.1007/BF01088283
- Author:
- Pena, L.; Cervera, M.; Juarez, J.; Navarro, A.; Pina, J.A.; Navarro, L.
- Source:
- Plant cell reports 1997 v.16 no.11 pp. 731-737
- ISSN:
- 0721-7714
- Subject:
- genetic transformation; gene transfer; geneticin; kanamycin; dose response; transgenic plants; Citrus aurantiifolia; Agrobacterium radiobacter; explants; gene expression; histochemistry; micropropagation; enzyme activity; culture media; beta-glucuronidase; light
- Abstract:
- ... We have previously developed procedures for the efficient production of sweet orange (Citrus sinensis L. Osbeck) and Carrizo citrange (C. sinensis L. Osbeck x Poncirus trifoliata L. Raf.) transgenic plants using an Agrobacterium tumefaciens-mediated transformation and shoot tip grafting in vitro regeneration system. We now report on the optimization of the cocultivation, regeneration and selection ...
- DOI:
- 10.1007/s002990050311
- https://doi.org/10.1007/s002990050311
- Author:
- Veylder, L. de.; Montagu, M. van.; Inze, D.
- Source:
- Plant & cell physiology 1997 v.38 no.5 pp. 568-577
- ISSN:
- 0032-0781
- Subject:
- herbicide safeners; developmental stages; branched chain amino acids; acetolactate synthase; genes; benzene; roots; dose response; gene expression; Arabidopsis thaliana; apical meristems; amino acid sequences; enzyme inhibitors; herbicide resistance; enzyme activity; histochemistry; transgenic plants; chlorsulfuron; beta-glucuronidase
- Abstract:
- ... The potential use of a new chemical-inducible gene expression system in Arabidopsis thaliana has been examined. The system is based on the maize In2-2 promoter which is activated by benzenesulfonamide herbicide safeners. Plants transformed with the beta-glucuronidase (gus) reporter gene under the control of the In2-2 promoter were grown in the presence of different safeners and the induced GUS act ...
- DOI:
- 10.1093/oxfordjournals.pcp.a029206
- PubMed:
- 9210333
- https://doi.org/10.1093/oxfordjournals.pcp.a029206
- Author:
- Holbrook, L.; Tung, P.; Ward, K.; Reid, D.M.; Abrams, S.; Lamb, N.; Quail, J.W.; Moloney, M.M.
- Source:
- Plant physiology 1997 v.114 no.2 pp. 419-428
- ISSN:
- 0032-0889
- Subject:
- Helianthus annuus; Brassica napus; measurement; chemical degradation; dose response; jasmonic acid; gene expression; Triticum aestivum; Nicotiana tabacum; esters; stereochemistry; enzyme activity; chemical structure; seed germination; transgenic plants; cotyledons; proteinase inhibitors; reporter genes; chemical constituents of plants; chlorophyll; beta-glucuronidase
- Abstract:
- ... The importance of the two chiral centers at C-3 and C-7 in the molecular structure of jasmonic acid in plant responses was investigated. We separated methyl jasmonate (MeJA) into (3R)- and (3S)-isomers with a fixed stereochemistry at C-3, but epimerization at C-7 is possible. The four isomers of the nonepimerizable analog 7-methyl MeJA were synthesized. These six esters and their corresponding aci ...
- Author:
- Mullins, K.V.; Llewellyn, D.J.; Hartney, V.J.; Strauss, S.; Dennis, E.S.
- Source:
- Plant cell reports 1997 v.16 no.11 pp. 787-791
- ISSN:
- 0721-7714
- Subject:
- benzyladenine; dose response; Agrobacterium radiobacter; explants; Eucalyptus marginata; gene expression; clones; naphthaleneacetic acid; Eucalyptus grandis; leaves; culture media; indole acetic acid; Eucalyptus microtheca; genetic transformation; gene transfer; kanamycin; Eucalyptus camaldulensis; transgenic plants; reporter genes; genetic variation; beta-glucuronidase
- Abstract:
- ... Reliable regeneration protocols for Eucalyptus camaldulensis using leaf explants from in vitro-grown plants have been developed. Out of the 24 clones tested 13 were regenerated and of these, 6 showed regeneration from more than 60% of the explants. Identical protocols were also successful in the regeneration of some clones of E. microtheca, E. ochrophloia, E. grandis and E. marginata, but at lower ...
- DOI:
- 10.1007/s002990050321
- https://doi.org/10.1007/s002990050321
- Author:
- Krens, F.A.; Keizer, L.C.P.; Capel, I.E.M.
- Source:
- Plant cell reports 1997 v.17 no.1 pp. 39-43
- ISSN:
- 0721-7714
- Subject:
- benzyladenine; genetic engineering; micropropagation; 2,4-D; Carum carvi; callus; shoots; dose response; hypocotyls; Agrobacterium radiobacter; explants; gene expression; naphthaleneacetic acid; metabolism; enzyme activity; culture media; genetic transformation; gene transfer; transgenic plants; cotyledons; beta-glucuronidase
- Abstract:
- ... Regeneration in caraway was obtained via two different routes. Hypocotyls showed delayed shoot formation after a callus phase and at relatively low frequencies. In contrast, high-frequency, direct regeneration occurred when cotyledonary node explants were used. Transient expression of beta-glucuronidase was monitored after inoculation of both explant types with Agrobacterium tumefaciens AGL0(pMOG4 ...
- DOI:
- 10.1007/s002990050348
- https://doi.org/10.1007/s002990050348