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You searched for: Source 1997 v.114 no.2 Remove constraint Source: 1997 v.114 no.2 Subject Nicotiana tabacum Remove constraint Subject: Nicotiana tabacum Subject enzyme activity Remove constraint Subject: enzyme activity Subject transgenic plants Remove constraint Subject: transgenic plants Text availability Citation in PubAg Remove constraint Text availability: Citation in PubAg Text Availability Citation in PubAg Remove constraint Text Availability: Citation in PubAg
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1. Importance of the chiral centers of jasmonic acid in the responses of plants. Activities and antagonism between natural and synthetic analogs

Author:
Holbrook, L.; Tung, P.; Ward, K.; Reid, D.M.; Abrams, S.; Lamb, N.; Quail, J.W.; Moloney, M.M.
Source:
Plant physiology 1997 v.114 no.2 pp. 419-428
ISSN:
0032-0889
Subject:
Helianthus annuus; Brassica napus; measurement; chemical degradation; dose response; jasmonic acid; gene expression; Triticum aestivum; Nicotiana tabacum; esters; stereochemistry; enzyme activity; chemical structure; seed germination; transgenic plants; cotyledons; proteinase inhibitors; reporter genes; chemical constituents of plants; chlorophyll; beta-glucuronidase
Abstract:
... The importance of the two chiral centers at C-3 and C-7 in the molecular structure of jasmonic acid in plant responses was investigated. We separated methyl jasmonate (MeJA) into (3R)- and (3S)-isomers with a fixed stereochemistry at C-3, but epimerization at C-7 is possible. The four isomers of the nonepimerizable analog 7-methyl MeJA were synthesized. These six esters and their corresponding aci ...

2. Overproduction of ascorbate peroxidase in the tobacco chloroplast does not provide protection against ozone

Author:
Torsethaugen, G.; Pitcher, L.H.; Zilinskas, B.A.; Pell, E.J.
Source:
Plant physiology 1997 v.114 no.2 pp. 529-537
ISSN:
0032-0889
Subject:
genetic transformation; gene transfer; biosynthesis; phytotoxicity; gas exchange; transgenic plants; Agrobacterium radiobacter; stress response; ozone; measurement; Nicotiana tabacum; enzyme activity; peroxidase; antioxidants; chloroplasts; ascorbic acid
Abstract:
... Transgenic tobacco (Nicotiana tabacum cv Bel W3) plants were used to test the hypothesis that protection from O3 injury could be conferred by overproduction of ascorbate peroxidase (APX) in the chloroplast. The 10-fold increase in soluble APX activity in the chloroplast was expected to alleviate an implied increase in oxidative potential and prevent damage caused by O3. Three different O3 exposure ...