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- Yang, Hui; Chen, Jixiang; Yang, Guanpin; Zhang, Xiao-Hua; Li, Yun
- FEMS microbiology letters 2007 v.267 no.1 pp. 56-63
- Escherichia coli; Vibrio anguillarum; active sites; cytotoxicity; enzyme activity; flounder; genes; metalloproteinases; mutants; pathogenesis; pathogens; proteolysis; site-directed mutagenesis; virulence; zinc
- ... The extracellular zinc metalloprotease, EmpA, is a putative virulence factor involved in pathogenicity of the fish pathogen Vibrio anguillarum. The 611-amino acid precursor of this enzyme is encoded by the empA gene. The residues His³⁴⁶, His³⁵⁰, Glu³⁷⁰, Glu³⁴⁷, His⁴²⁹, Tyr³⁶¹ and Asp⁴¹⁷ are highly conserved and putatively function together at the active site of the enzyme. In this study, empA was ...